Experiment: McCas_formate_complete Polymyxin B sulfate 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00105 and MMJJ_RS00110 are separated by 21 nucleotides MMJJ_RS00110 and MMJJ_RS00115 are separated by 28 nucleotides MMJJ_RS00115 and MMJJ_RS00120 are separated by 33 nucleotides
MMJJ_RS00105: MMJJ_RS00105 - adenylosuccinate lyase, at 19,351 to 20,700
_RS00105
MMJJ_RS00110: MMJJ_RS00110 - ATP-dependent DNA ligase, at 20,722 to 22,443
_RS00110
MMJJ_RS00115: MMJJ_RS00115 - hypothetical protein, at 22,472 to 22,888
_RS00115
MMJJ_RS00120: MMJJ_RS00120 - histidinol dehydrogenase, at 22,922 to 24,196
_RS00120
Position (kb) 20
21
22
23 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 21.095 kb on + strand at 21.711 kb on - strand, within MMJJ_RS00110 at 21.867 kb on + strand, within MMJJ_RS00110 at 21.868 kb on - strand, within MMJJ_RS00110 at 22.474 kb on + strand at 22.474 kb on + strand at 22.474 kb on + strand at 22.492 kb on - strand at 22.496 kb on - strand at 22.623 kb on + strand, within MMJJ_RS00115 at 22.623 kb on + strand, within MMJJ_RS00115 at 22.626 kb on - strand, within MMJJ_RS00115 at 22.626 kb on - strand, within MMJJ_RS00115 at 22.626 kb on - strand, within MMJJ_RS00115 at 22.627 kb on + strand, within MMJJ_RS00115 at 22.627 kb on + strand, within MMJJ_RS00115 at 22.693 kb on - strand, within MMJJ_RS00115 at 22.714 kb on - strand, within MMJJ_RS00115 at 22.814 kb on + strand, within MMJJ_RS00115 at 22.865 kb on + strand at 22.868 kb on - strand at 22.869 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Polymyxin B sulfate 0.05 mM remove 21,095 + +0.6 21,711 - MMJJ_RS00110 0.57 -2.0 21,867 + MMJJ_RS00110 0.66 -2.0 21,868 - MMJJ_RS00110 0.67 +0.6 22,474 + -2.8 22,474 + -3.0 22,474 + -0.4 22,492 - -4.3 22,496 - -1.0 22,623 + MMJJ_RS00115 0.36 -1.0 22,623 + MMJJ_RS00115 0.36 -4.6 22,626 - MMJJ_RS00115 0.37 -0.7 22,626 - MMJJ_RS00115 0.37 +0.9 22,626 - MMJJ_RS00115 0.37 -0.6 22,627 + MMJJ_RS00115 0.37 -2.9 22,627 + MMJJ_RS00115 0.37 -0.2 22,693 - MMJJ_RS00115 0.53 -4.1 22,714 - MMJJ_RS00115 0.58 -1.2 22,814 + MMJJ_RS00115 0.82 -3.2 22,865 + -2.2 22,868 - -2.8 22,869 + -0.3
Or see this region's nucleotide sequence