Experiment: McCas_formate_complete phosphite 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03305 and MMJJ_RS03310 are separated by 14 nucleotides MMJJ_RS03310 and MMJJ_RS03315 are separated by 211 nucleotides
MMJJ_RS03305: MMJJ_RS03305 - zincin-like metallopeptidase domain-containing protein, at 627,263 to 629,320
_RS03305
MMJJ_RS03310: MMJJ_RS03310 - hypothetical protein, at 629,335 to 629,553
_RS03310
MMJJ_RS03315: MMJJ_RS03315 - hypothetical protein, at 629,765 to 631,825
_RS03315
Position (kb) 627
628
629
630 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 626.284 kb on - strand at 626.314 kb on - strand at 626.315 kb on + strand at 626.315 kb on + strand at 626.333 kb on - strand at 626.334 kb on + strand at 626.369 kb on - strand at 626.373 kb on - strand at 626.386 kb on - strand at 626.397 kb on + strand at 626.408 kb on - strand at 626.431 kb on - strand at 626.431 kb on - strand at 626.443 kb on - strand at 626.449 kb on - strand at 626.450 kb on + strand at 626.450 kb on + strand at 626.454 kb on + strand at 626.455 kb on - strand at 626.455 kb on - strand at 626.456 kb on + strand at 626.456 kb on + strand at 626.460 kb on - strand at 626.460 kb on - strand at 626.460 kb on - strand at 626.460 kb on - strand at 626.461 kb on + strand at 626.461 kb on + strand at 626.466 kb on + strand at 626.466 kb on + strand at 626.468 kb on - strand at 626.475 kb on + strand at 626.513 kb on - strand at 626.517 kb on - strand at 626.530 kb on - strand at 626.530 kb on - strand at 626.531 kb on + strand at 626.626 kb on - strand at 626.688 kb on + strand at 626.688 kb on + strand at 626.688 kb on + strand at 626.688 kb on + strand at 626.713 kb on - strand at 626.714 kb on + strand at 626.714 kb on + strand at 626.720 kb on - strand at 626.721 kb on + strand at 626.750 kb on - strand at 626.751 kb on + strand at 626.754 kb on + strand at 626.754 kb on + strand at 626.756 kb on - strand at 626.756 kb on - strand at 626.758 kb on - strand at 626.758 kb on - strand at 626.758 kb on - strand at 626.759 kb on + strand at 626.759 kb on + strand at 626.759 kb on + strand at 626.811 kb on - strand at 626.812 kb on + strand at 626.836 kb on - strand at 626.924 kb on + strand at 626.929 kb on + strand at 627.025 kb on + strand at 627.054 kb on + strand at 627.083 kb on - strand at 627.084 kb on + strand at 627.084 kb on + strand at 627.084 kb on + strand at 627.084 kb on + strand at 627.084 kb on + strand at 627.084 kb on + strand at 627.085 kb on - strand at 627.086 kb on + strand at 627.086 kb on + strand at 627.086 kb on + strand at 627.088 kb on + strand at 627.102 kb on + strand at 627.121 kb on - strand at 627.121 kb on - strand at 627.121 kb on - strand at 627.122 kb on + strand at 627.245 kb on + strand at 627.310 kb on - strand at 627.371 kb on - strand at 627.431 kb on - strand at 627.436 kb on + strand at 627.484 kb on - strand, within MMJJ_RS03305 at 627.485 kb on + strand, within MMJJ_RS03305 at 627.509 kb on + strand, within MMJJ_RS03305 at 627.553 kb on + strand, within MMJJ_RS03305 at 627.561 kb on - strand, within MMJJ_RS03305 at 627.584 kb on - strand, within MMJJ_RS03305 at 627.587 kb on - strand, within MMJJ_RS03305 at 627.588 kb on + strand, within MMJJ_RS03305 at 627.588 kb on + strand, within MMJJ_RS03305 at 627.589 kb on - strand, within MMJJ_RS03305 at 627.589 kb on - strand, within MMJJ_RS03305 at 627.590 kb on + strand, within MMJJ_RS03305 at 627.601 kb on - strand, within MMJJ_RS03305 at 627.601 kb on - strand, within MMJJ_RS03305 at 627.601 kb on - strand, within MMJJ_RS03305 at 627.628 kb on - strand, within MMJJ_RS03305 at 627.651 kb on - strand, within MMJJ_RS03305 at 627.659 kb on + strand, within MMJJ_RS03305 at 627.687 kb on - strand, within MMJJ_RS03305 at 627.740 kb on - strand, within MMJJ_RS03305 at 627.740 kb on - strand, within MMJJ_RS03305 at 627.741 kb on + strand, within MMJJ_RS03305 at 627.763 kb on + strand, within MMJJ_RS03305 at 627.795 kb on - strand, within MMJJ_RS03305 at 627.839 kb on + strand, within MMJJ_RS03305 at 627.998 kb on - strand, within MMJJ_RS03305 at 628.000 kb on - strand, within MMJJ_RS03305 at 628.000 kb on - strand, within MMJJ_RS03305 at 628.000 kb on - strand, within MMJJ_RS03305 at 628.001 kb on + strand, within MMJJ_RS03305 at 628.002 kb on - strand, within MMJJ_RS03305 at 628.002 kb on - strand, within MMJJ_RS03305 at 628.003 kb on + strand, within MMJJ_RS03305 at 628.003 kb on + strand, within MMJJ_RS03305 at 628.003 kb on + strand, within MMJJ_RS03305 at 628.003 kb on + strand, within MMJJ_RS03305 at 628.036 kb on - strand, within MMJJ_RS03305 at 628.037 kb on + strand, within MMJJ_RS03305 at 628.037 kb on + strand, within MMJJ_RS03305 at 628.039 kb on - strand, within MMJJ_RS03305 at 628.040 kb on + strand, within MMJJ_RS03305 at 628.150 kb on - strand, within MMJJ_RS03305 at 628.180 kb on - strand, within MMJJ_RS03305 at 628.263 kb on - strand, within MMJJ_RS03305 at 628.373 kb on + strand, within MMJJ_RS03305 at 628.426 kb on + strand, within MMJJ_RS03305 at 628.430 kb on + strand, within MMJJ_RS03305 at 628.482 kb on - strand, within MMJJ_RS03305 at 628.483 kb on + strand, within MMJJ_RS03305 at 628.506 kb on - strand, within MMJJ_RS03305 at 628.506 kb on - strand, within MMJJ_RS03305 at 628.506 kb on - strand, within MMJJ_RS03305 at 628.507 kb on + strand, within MMJJ_RS03305 at 628.509 kb on - strand, within MMJJ_RS03305 at 628.607 kb on + strand, within MMJJ_RS03305 at 628.614 kb on - strand, within MMJJ_RS03305 at 628.929 kb on - strand, within MMJJ_RS03305 at 628.985 kb on - strand, within MMJJ_RS03305 at 628.986 kb on + strand, within MMJJ_RS03305 at 629.031 kb on - strand, within MMJJ_RS03305 at 629.031 kb on - strand, within MMJJ_RS03305 at 629.031 kb on - strand, within MMJJ_RS03305 at 629.031 kb on - strand, within MMJJ_RS03305 at 629.032 kb on + strand, within MMJJ_RS03305 at 629.041 kb on + strand, within MMJJ_RS03305 at 629.051 kb on - strand, within MMJJ_RS03305 at 629.052 kb on + strand, within MMJJ_RS03305 at 629.058 kb on - strand, within MMJJ_RS03305 at 629.058 kb on - strand, within MMJJ_RS03305 at 629.089 kb on - strand, within MMJJ_RS03305 at 629.111 kb on + strand, within MMJJ_RS03305 at 629.126 kb on - strand at 629.165 kb on - strand at 629.167 kb on - strand at 629.167 kb on - strand at 629.168 kb on + strand at 629.168 kb on + strand at 629.168 kb on + strand at 629.168 kb on + strand at 629.222 kb on + strand at 629.227 kb on - strand at 629.227 kb on - strand at 629.227 kb on - strand at 629.227 kb on - strand at 629.227 kb on - strand at 629.227 kb on - strand at 629.228 kb on + strand at 629.228 kb on + strand at 629.228 kb on + strand at 629.230 kb on + strand at 629.235 kb on - strand at 629.252 kb on - strand at 629.312 kb on - strand at 629.313 kb on + strand at 629.318 kb on - strand at 629.324 kb on + strand at 629.331 kb on - strand at 629.367 kb on + strand, within MMJJ_RS03310 at 629.384 kb on - strand, within MMJJ_RS03310 at 629.385 kb on + strand, within MMJJ_RS03310 at 629.427 kb on - strand, within MMJJ_RS03310 at 629.436 kb on - strand, within MMJJ_RS03310 at 629.436 kb on - strand, within MMJJ_RS03310 at 629.448 kb on + strand, within MMJJ_RS03310 at 629.503 kb on - strand, within MMJJ_RS03310 at 629.503 kb on - strand, within MMJJ_RS03310 at 629.504 kb on + strand, within MMJJ_RS03310 at 629.505 kb on - strand at 629.550 kb on + strand at 629.551 kb on - strand at 629.552 kb on + strand at 629.590 kb on + strand at 629.590 kb on + strand at 629.630 kb on - strand at 629.633 kb on + strand at 629.644 kb on + strand at 629.804 kb on - strand at 629.813 kb on + strand at 629.861 kb on - strand at 629.861 kb on - strand at 629.861 kb on - strand at 629.861 kb on - strand at 629.862 kb on + strand at 629.862 kb on + strand at 629.864 kb on - strand at 629.864 kb on - strand at 629.873 kb on - strand at 629.873 kb on - strand at 629.899 kb on - strand at 629.907 kb on + strand at 629.910 kb on + strand at 629.968 kb on - strand at 629.997 kb on + strand, within MMJJ_RS03315 at 630.021 kb on - strand, within MMJJ_RS03315 at 630.021 kb on - strand, within MMJJ_RS03315 at 630.021 kb on - strand, within MMJJ_RS03315 at 630.025 kb on + strand, within MMJJ_RS03315 at 630.067 kb on + strand, within MMJJ_RS03315 at 630.067 kb on + strand, within MMJJ_RS03315 at 630.067 kb on + strand, within MMJJ_RS03315 at 630.069 kb on - strand, within MMJJ_RS03315 at 630.070 kb on + strand, within MMJJ_RS03315 at 630.094 kb on - strand, within MMJJ_RS03315 at 630.095 kb on + strand, within MMJJ_RS03315 at 630.110 kb on + strand, within MMJJ_RS03315 at 630.319 kb on - strand, within MMJJ_RS03315 at 630.320 kb on + strand, within MMJJ_RS03315 at 630.320 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete phosphite 25 mM remove 626,284 - +1.1 626,314 - -0.9 626,315 + -0.8 626,315 + -0.1 626,333 - +0.4 626,334 + -3.0 626,369 - +1.0 626,373 - +0.7 626,386 - -1.0 626,397 + +4.2 626,408 - -2.9 626,431 - +0.2 626,431 - -2.4 626,443 - -0.4 626,449 - +4.5 626,450 + +0.4 626,450 + +2.4 626,454 + +0.2 626,455 - +0.2 626,455 - +1.4 626,456 + -3.6 626,456 + -1.3 626,460 - -1.1 626,460 - -2.2 626,460 - +1.6 626,460 - +0.2 626,461 + +0.7 626,461 + -0.3 626,466 + +1.6 626,466 + -0.9 626,468 - +0.5 626,475 + -2.9 626,513 - +1.6 626,517 - -0.7 626,530 - -0.3 626,530 - +3.3 626,531 + +1.5 626,626 - -2.2 626,688 + +0.7 626,688 + -0.9 626,688 + -2.1 626,688 + -1.9 626,713 - -0.1 626,714 + -0.5 626,714 + +0.2 626,720 - -2.2 626,721 + +1.0 626,750 - -0.4 626,751 + +0.5 626,754 + +0.4 626,754 + -0.5 626,756 - +0.8 626,756 - -0.4 626,758 - -0.0 626,758 - -1.3 626,758 - -0.9 626,759 + -0.9 626,759 + -0.4 626,759 + -1.6 626,811 - +0.2 626,812 + +0.3 626,836 - +0.4 626,924 + -1.0 626,929 + -0.5 627,025 + -1.9 627,054 + +0.6 627,083 - +0.1 627,084 + +0.5 627,084 + +0.1 627,084 + +0.2 627,084 + -0.9 627,084 + +0.0 627,084 + -0.9 627,085 - +0.9 627,086 + +2.1 627,086 + -0.3 627,086 + -2.6 627,088 + -2.8 627,102 + +1.1 627,121 - -0.6 627,121 - +0.5 627,121 - +0.3 627,122 + +1.9 627,245 + +0.5 627,310 - +0.6 627,371 - -0.9 627,431 - +0.7 627,436 + -2.1 627,484 - MMJJ_RS03305 0.11 -2.2 627,485 + MMJJ_RS03305 0.11 +0.0 627,509 + MMJJ_RS03305 0.12 +0.2 627,553 + MMJJ_RS03305 0.14 +0.7 627,561 - MMJJ_RS03305 0.14 -0.4 627,584 - MMJJ_RS03305 0.16 -1.3 627,587 - MMJJ_RS03305 0.16 -0.5 627,588 + MMJJ_RS03305 0.16 -0.5 627,588 + MMJJ_RS03305 0.16 -0.3 627,589 - MMJJ_RS03305 0.16 +4.3 627,589 - MMJJ_RS03305 0.16 -1.6 627,590 + MMJJ_RS03305 0.16 -0.6 627,601 - MMJJ_RS03305 0.16 -0.8 627,601 - MMJJ_RS03305 0.16 +2.3 627,601 - MMJJ_RS03305 0.16 +1.0 627,628 - MMJJ_RS03305 0.18 -0.7 627,651 - MMJJ_RS03305 0.19 +0.1 627,659 + MMJJ_RS03305 0.19 -0.3 627,687 - MMJJ_RS03305 0.21 +1.9 627,740 - MMJJ_RS03305 0.23 -2.0 627,740 - MMJJ_RS03305 0.23 -0.2 627,741 + MMJJ_RS03305 0.23 +0.5 627,763 + MMJJ_RS03305 0.24 +0.5 627,795 - MMJJ_RS03305 0.26 -0.4 627,839 + MMJJ_RS03305 0.28 -0.5 627,998 - MMJJ_RS03305 0.36 +0.0 628,000 - MMJJ_RS03305 0.36 +0.8 628,000 - MMJJ_RS03305 0.36 +0.6 628,000 - MMJJ_RS03305 0.36 +0.1 628,001 + MMJJ_RS03305 0.36 +0.4 628,002 - MMJJ_RS03305 0.36 -1.9 628,002 - MMJJ_RS03305 0.36 -0.6 628,003 + MMJJ_RS03305 0.36 +0.5 628,003 + MMJJ_RS03305 0.36 -1.6 628,003 + MMJJ_RS03305 0.36 -1.2 628,003 + MMJJ_RS03305 0.36 +0.2 628,036 - MMJJ_RS03305 0.38 -1.9 628,037 + MMJJ_RS03305 0.38 +1.0 628,037 + MMJJ_RS03305 0.38 -0.3 628,039 - MMJJ_RS03305 0.38 +0.4 628,040 + MMJJ_RS03305 0.38 +0.4 628,150 - MMJJ_RS03305 0.43 -1.3 628,180 - MMJJ_RS03305 0.45 -0.5 628,263 - MMJJ_RS03305 0.49 -1.0 628,373 + MMJJ_RS03305 0.54 +1.0 628,426 + MMJJ_RS03305 0.57 -0.6 628,430 + MMJJ_RS03305 0.57 +0.3 628,482 - MMJJ_RS03305 0.59 -0.5 628,483 + MMJJ_RS03305 0.59 -1.3 628,506 - MMJJ_RS03305 0.60 +0.0 628,506 - MMJJ_RS03305 0.60 +0.9 628,506 - MMJJ_RS03305 0.60 -1.2 628,507 + MMJJ_RS03305 0.60 +0.1 628,509 - MMJJ_RS03305 0.61 -0.0 628,607 + MMJJ_RS03305 0.65 +1.3 628,614 - MMJJ_RS03305 0.66 -1.5 628,929 - MMJJ_RS03305 0.81 -3.0 628,985 - MMJJ_RS03305 0.84 -0.8 628,986 + MMJJ_RS03305 0.84 +0.5 629,031 - MMJJ_RS03305 0.86 +1.2 629,031 - MMJJ_RS03305 0.86 +1.0 629,031 - MMJJ_RS03305 0.86 -0.2 629,031 - MMJJ_RS03305 0.86 +1.3 629,032 + MMJJ_RS03305 0.86 -2.9 629,041 + MMJJ_RS03305 0.86 +0.4 629,051 - MMJJ_RS03305 0.87 -1.4 629,052 + MMJJ_RS03305 0.87 -0.3 629,058 - MMJJ_RS03305 0.87 -1.9 629,058 - MMJJ_RS03305 0.87 +1.6 629,089 - MMJJ_RS03305 0.89 +0.4 629,111 + MMJJ_RS03305 0.90 +0.2 629,126 - -0.4 629,165 - -1.5 629,167 - -2.7 629,167 - -0.2 629,168 + +0.7 629,168 + +0.1 629,168 + -1.9 629,168 + +3.7 629,222 + +1.3 629,227 - +0.6 629,227 - -0.7 629,227 - +0.5 629,227 - -1.3 629,227 - -1.1 629,227 - -2.3 629,228 + +1.6 629,228 + +0.4 629,228 + +0.3 629,230 + +0.7 629,235 - -0.5 629,252 - +0.8 629,312 - -2.9 629,313 + +2.8 629,318 - -0.2 629,324 + +0.0 629,331 - -0.6 629,367 + MMJJ_RS03310 0.15 -0.9 629,384 - MMJJ_RS03310 0.22 -2.5 629,385 + MMJJ_RS03310 0.23 -0.3 629,427 - MMJJ_RS03310 0.42 -0.4 629,436 - MMJJ_RS03310 0.46 -1.3 629,436 - MMJJ_RS03310 0.46 -1.3 629,448 + MMJJ_RS03310 0.52 -0.3 629,503 - MMJJ_RS03310 0.77 -0.9 629,503 - MMJJ_RS03310 0.77 -1.3 629,504 + MMJJ_RS03310 0.77 -1.6 629,505 - -0.3 629,550 + +1.6 629,551 - +3.4 629,552 + +0.6 629,590 + +3.3 629,590 + +1.3 629,630 - +0.7 629,633 + +0.2 629,644 + +0.8 629,804 - -2.1 629,813 + +0.6 629,861 - +2.2 629,861 - -2.2 629,861 - +0.2 629,861 - -0.5 629,862 + +0.6 629,862 + +0.0 629,864 - +2.5 629,864 - +0.6 629,873 - -1.6 629,873 - -4.2 629,899 - -2.3 629,907 + -1.1 629,910 + -0.4 629,968 - -1.2 629,997 + MMJJ_RS03315 0.11 -0.6 630,021 - MMJJ_RS03315 0.12 +1.0 630,021 - MMJJ_RS03315 0.12 -2.6 630,021 - MMJJ_RS03315 0.12 -1.5 630,025 + MMJJ_RS03315 0.13 +1.1 630,067 + MMJJ_RS03315 0.15 +0.3 630,067 + MMJJ_RS03315 0.15 -1.7 630,067 + MMJJ_RS03315 0.15 -0.9 630,069 - MMJJ_RS03315 0.15 +0.4 630,070 + MMJJ_RS03315 0.15 +1.0 630,094 - MMJJ_RS03315 0.16 +0.2 630,095 + MMJJ_RS03315 0.16 +0.6 630,110 + MMJJ_RS03315 0.17 +0.1 630,319 - MMJJ_RS03315 0.27 +0.3 630,320 + MMJJ_RS03315 0.27 +1.7 630,320 + +0.7
Or see this region's nucleotide sequence