Experiment: McCas_formate_complete phosphite 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02120 and MMJJ_RS02125 are separated by 118 nucleotides MMJJ_RS02125 and MMJJ_RS02130 are separated by 9 nucleotides MMJJ_RS02130 and MMJJ_RS02135 are separated by 1 nucleotides MMJJ_RS02135 and MMJJ_RS02140 overlap by 4 nucleotides MMJJ_RS02140 and MMJJ_RS02145 are separated by 43 nucleotides
MMJJ_RS02120: MMJJ_RS02120 - DJ-1/PfpI family protein, at 404,820 to 405,335
_RS02120
MMJJ_RS02125: MMJJ_RS02125 - hypothetical protein, at 405,454 to 405,750
_RS02125
MMJJ_RS02130: MMJJ_RS02130 - UPF0146 family protein, at 405,760 to 406,173
_RS02130
MMJJ_RS02135: MMJJ_RS02135 - TatD family hydrolase, at 406,175 to 407,017
_RS02135
MMJJ_RS02140: MMJJ_RS02140 - preflagellin peptidase FlaK, at 407,014 to 407,706
_RS02140
MMJJ_RS02145: MMJJ_RS02145 - class I SAM-dependent methyltransferase, at 407,750 to 408,352
_RS02145
Position (kb) 406
407
408 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 405.325 kb on + strand at 405.361 kb on + strand at 405.361 kb on + strand at 405.364 kb on - strand at 405.364 kb on - strand at 405.365 kb on + strand at 405.365 kb on + strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.370 kb on - strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.371 kb on + strand at 405.372 kb on - strand at 405.372 kb on - strand at 405.373 kb on + strand at 405.373 kb on + strand at 405.374 kb on - strand at 405.375 kb on + strand at 405.375 kb on + strand at 405.375 kb on + strand at 405.375 kb on + strand at 405.379 kb on - strand at 405.385 kb on + strand at 405.398 kb on - strand at 405.398 kb on - strand at 405.398 kb on - strand at 405.398 kb on - strand at 405.426 kb on - strand at 405.427 kb on + strand at 405.442 kb on + strand at 405.513 kb on - strand, within MMJJ_RS02125 at 405.513 kb on - strand, within MMJJ_RS02125 at 405.513 kb on - strand, within MMJJ_RS02125 at 405.514 kb on + strand, within MMJJ_RS02125 at 405.583 kb on - strand, within MMJJ_RS02125 at 405.594 kb on - strand, within MMJJ_RS02125 at 405.749 kb on + strand at 405.779 kb on + strand at 405.779 kb on + strand at 405.782 kb on - strand at 405.818 kb on - strand at 405.903 kb on - strand, within MMJJ_RS02130 at 406.172 kb on + strand at 406.231 kb on + strand at 406.294 kb on - strand, within MMJJ_RS02135 at 406.295 kb on + strand, within MMJJ_RS02135 at 406.304 kb on + strand, within MMJJ_RS02135 at 406.315 kb on - strand, within MMJJ_RS02135 at 406.315 kb on - strand, within MMJJ_RS02135 at 406.316 kb on + strand, within MMJJ_RS02135 at 406.357 kb on + strand, within MMJJ_RS02135 at 406.369 kb on + strand, within MMJJ_RS02135 at 406.476 kb on - strand, within MMJJ_RS02135 at 406.476 kb on - strand, within MMJJ_RS02135 at 406.476 kb on - strand, within MMJJ_RS02135 at 406.477 kb on + strand, within MMJJ_RS02135 at 406.477 kb on + strand, within MMJJ_RS02135 at 406.477 kb on + strand, within MMJJ_RS02135 at 406.477 kb on + strand, within MMJJ_RS02135 at 406.501 kb on - strand, within MMJJ_RS02135 at 406.501 kb on - strand, within MMJJ_RS02135 at 406.510 kb on + strand, within MMJJ_RS02135 at 406.510 kb on + strand, within MMJJ_RS02135 at 406.575 kb on - strand, within MMJJ_RS02135 at 406.605 kb on + strand, within MMJJ_RS02135 at 406.628 kb on + strand, within MMJJ_RS02135 at 406.758 kb on - strand, within MMJJ_RS02135 at 406.856 kb on - strand, within MMJJ_RS02135 at 407.039 kb on + strand at 407.039 kb on + strand at 407.065 kb on - strand at 407.066 kb on + strand at 407.066 kb on + strand at 407.069 kb on - strand at 407.069 kb on - strand at 407.069 kb on - strand at 407.069 kb on - strand at 407.070 kb on + strand at 407.070 kb on + strand at 407.070 kb on + strand at 407.070 kb on + strand at 407.074 kb on - strand at 407.075 kb on + strand at 407.080 kb on - strand at 407.091 kb on - strand, within MMJJ_RS02140 at 407.110 kb on + strand, within MMJJ_RS02140 at 407.117 kb on + strand, within MMJJ_RS02140 at 407.135 kb on - strand, within MMJJ_RS02140 at 407.136 kb on + strand, within MMJJ_RS02140 at 407.381 kb on - strand, within MMJJ_RS02140 at 407.382 kb on + strand, within MMJJ_RS02140 at 407.414 kb on - strand, within MMJJ_RS02140 at 407.414 kb on - strand, within MMJJ_RS02140 at 407.414 kb on - strand, within MMJJ_RS02140 at 407.414 kb on - strand, within MMJJ_RS02140 at 407.415 kb on + strand, within MMJJ_RS02140 at 407.415 kb on + strand, within MMJJ_RS02140 at 407.415 kb on + strand, within MMJJ_RS02140 at 407.415 kb on + strand, within MMJJ_RS02140 at 407.415 kb on + strand, within MMJJ_RS02140 at 407.415 kb on + strand, within MMJJ_RS02140 at 407.492 kb on + strand, within MMJJ_RS02140 at 407.502 kb on - strand, within MMJJ_RS02140 at 407.504 kb on - strand, within MMJJ_RS02140 at 407.627 kb on - strand, within MMJJ_RS02140 at 407.628 kb on + strand, within MMJJ_RS02140 at 407.628 kb on + strand, within MMJJ_RS02140 at 407.668 kb on - strand at 407.684 kb on + strand at 407.697 kb on + strand at 407.697 kb on + strand at 407.725 kb on - strand at 407.730 kb on + strand at 407.730 kb on + strand at 407.730 kb on + strand at 407.741 kb on + strand at 407.799 kb on + strand at 407.804 kb on + strand at 407.837 kb on + strand, within MMJJ_RS02145 at 407.838 kb on - strand, within MMJJ_RS02145 at 407.843 kb on + strand, within MMJJ_RS02145 at 407.903 kb on - strand, within MMJJ_RS02145 at 407.940 kb on + strand, within MMJJ_RS02145 at 407.958 kb on + strand, within MMJJ_RS02145 at 407.974 kb on - strand, within MMJJ_RS02145 at 407.979 kb on + strand, within MMJJ_RS02145 at 407.979 kb on + strand, within MMJJ_RS02145 at 408.000 kb on + strand, within MMJJ_RS02145 at 408.012 kb on + strand, within MMJJ_RS02145 at 408.012 kb on + strand, within MMJJ_RS02145
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete phosphite 25 mM remove 405,325 + -3.1 405,361 + +1.0 405,361 + +1.1 405,364 - +0.9 405,364 - -1.7 405,365 + +1.9 405,365 + -4.4 405,370 - -0.5 405,370 - -0.3 405,370 - -0.9 405,370 - -0.5 405,370 - +1.0 405,370 - -2.5 405,370 - -2.0 405,370 - -0.1 405,370 - +0.2 405,370 - -2.2 405,370 - +1.4 405,370 - -0.1 405,370 - +1.0 405,371 + -0.0 405,371 + +2.2 405,371 + +0.7 405,371 + +0.4 405,371 + -1.6 405,371 + -1.7 405,371 + +0.5 405,371 + +1.3 405,371 + +2.1 405,371 + -0.2 405,371 + -0.4 405,371 + -0.9 405,371 + -2.9 405,371 + -0.3 405,371 + +0.6 405,371 + +0.2 405,371 + +3.9 405,371 + -1.2 405,372 - +2.0 405,372 - +1.4 405,373 + -0.2 405,373 + -0.2 405,374 - -1.2 405,375 + -0.1 405,375 + -2.2 405,375 + -0.8 405,375 + +0.7 405,379 - +0.6 405,385 + -1.0 405,398 - +1.1 405,398 - +1.1 405,398 - +0.8 405,398 - +0.5 405,426 - +1.6 405,427 + -0.1 405,442 + +1.8 405,513 - MMJJ_RS02125 0.20 +0.5 405,513 - MMJJ_RS02125 0.20 -0.8 405,513 - MMJJ_RS02125 0.20 -0.2 405,514 + MMJJ_RS02125 0.20 +0.1 405,583 - MMJJ_RS02125 0.43 +1.4 405,594 - MMJJ_RS02125 0.47 -1.4 405,749 + +0.4 405,779 + -2.1 405,779 + -2.4 405,782 - -0.4 405,818 - -0.4 405,903 - MMJJ_RS02130 0.35 +1.6 406,172 + -0.3 406,231 + -1.5 406,294 - MMJJ_RS02135 0.14 -1.6 406,295 + MMJJ_RS02135 0.14 +0.8 406,304 + MMJJ_RS02135 0.15 +0.1 406,315 - MMJJ_RS02135 0.17 -2.4 406,315 - MMJJ_RS02135 0.17 +2.8 406,316 + MMJJ_RS02135 0.17 -1.1 406,357 + MMJJ_RS02135 0.22 -0.5 406,369 + MMJJ_RS02135 0.23 -1.0 406,476 - MMJJ_RS02135 0.36 -2.6 406,476 - MMJJ_RS02135 0.36 -0.5 406,476 - MMJJ_RS02135 0.36 +0.6 406,477 + MMJJ_RS02135 0.36 +0.3 406,477 + MMJJ_RS02135 0.36 -0.2 406,477 + MMJJ_RS02135 0.36 -0.2 406,477 + MMJJ_RS02135 0.36 -0.1 406,501 - MMJJ_RS02135 0.39 +0.4 406,501 - MMJJ_RS02135 0.39 +0.3 406,510 + MMJJ_RS02135 0.40 +2.5 406,510 + MMJJ_RS02135 0.40 +0.7 406,575 - MMJJ_RS02135 0.47 -0.3 406,605 + MMJJ_RS02135 0.51 -0.5 406,628 + MMJJ_RS02135 0.54 -0.6 406,758 - MMJJ_RS02135 0.69 +0.3 406,856 - MMJJ_RS02135 0.81 -2.0 407,039 + -0.7 407,039 + +1.0 407,065 - -3.9 407,066 + -1.5 407,066 + +0.6 407,069 - -1.1 407,069 - +0.6 407,069 - -0.9 407,069 - -2.0 407,070 + -1.0 407,070 + -0.7 407,070 + -0.7 407,070 + -0.0 407,074 - -3.4 407,075 + -0.2 407,080 - +0.8 407,091 - MMJJ_RS02140 0.11 -0.8 407,110 + MMJJ_RS02140 0.14 -0.5 407,117 + MMJJ_RS02140 0.15 -1.0 407,135 - MMJJ_RS02140 0.17 -1.7 407,136 + MMJJ_RS02140 0.18 -1.5 407,381 - MMJJ_RS02140 0.53 -4.4 407,382 + MMJJ_RS02140 0.53 +0.8 407,414 - MMJJ_RS02140 0.58 -1.2 407,414 - MMJJ_RS02140 0.58 -1.9 407,414 - MMJJ_RS02140 0.58 -1.6 407,414 - MMJJ_RS02140 0.58 -0.5 407,415 + MMJJ_RS02140 0.58 +1.6 407,415 + MMJJ_RS02140 0.58 -0.7 407,415 + MMJJ_RS02140 0.58 -0.7 407,415 + MMJJ_RS02140 0.58 -1.2 407,415 + MMJJ_RS02140 0.58 -1.2 407,415 + MMJJ_RS02140 0.58 +0.6 407,492 + MMJJ_RS02140 0.69 -0.9 407,502 - MMJJ_RS02140 0.70 -2.1 407,504 - MMJJ_RS02140 0.71 -0.9 407,627 - MMJJ_RS02140 0.88 +0.6 407,628 + MMJJ_RS02140 0.89 +0.8 407,628 + MMJJ_RS02140 0.89 +1.6 407,668 - +0.6 407,684 + +1.4 407,697 + +1.8 407,697 + -0.1 407,725 - +1.0 407,730 + +1.2 407,730 + -0.6 407,730 + +0.9 407,741 + -2.2 407,799 + -0.9 407,804 + +0.1 407,837 + MMJJ_RS02145 0.14 +2.4 407,838 - MMJJ_RS02145 0.15 +1.0 407,843 + MMJJ_RS02145 0.15 -2.5 407,903 - MMJJ_RS02145 0.25 +1.3 407,940 + MMJJ_RS02145 0.32 +0.9 407,958 + MMJJ_RS02145 0.34 +3.8 407,974 - MMJJ_RS02145 0.37 -0.0 407,979 + MMJJ_RS02145 0.38 -0.1 407,979 + MMJJ_RS02145 0.38 -0.8 408,000 + MMJJ_RS02145 0.41 -2.2 408,012 + MMJJ_RS02145 0.43 -0.3 408,012 + MMJJ_RS02145 0.43 -1.1
Or see this region's nucleotide sequence