Experiment: McCas_formate_complete Spiramycin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08680 and MMJJ_RS08685 are separated by 12 nucleotides MMJJ_RS08685 and MMJJ_RS08690 are separated by 80 nucleotides MMJJ_RS08690 and MMJJ_RS08695 are separated by 242 nucleotides
MMJJ_RS08680: MMJJ_RS08680 - phosphate ABC transporter permease PstA, at 1,596,695 to 1,597,561
_RS08680
MMJJ_RS08685: MMJJ_RS08685 - phosphate ABC transporter permease subunit PstC, at 1,597,574 to 1,598,449
_RS08685
MMJJ_RS08690: MMJJ_RS08690 - phosphate ABC transporter substrate-binding protein, at 1,598,530 to 1,599,372
_RS08690
MMJJ_RS08695: MMJJ_RS08695 - phosphoenolpyruvate synthase, at 1,599,615 to 1,601,891
_RS08695
Position (kb)
1598
1599
1600 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1597.555 kb on - strand at 1597.556 kb on + strand at 1597.607 kb on + strand at 1597.607 kb on + strand at 1597.609 kb on + strand at 1597.617 kb on + strand at 1597.620 kb on + strand at 1597.620 kb on + strand at 1597.640 kb on - strand at 1597.650 kb on + strand at 1597.675 kb on + strand, within MMJJ_RS08685 at 1597.708 kb on - strand, within MMJJ_RS08685 at 1597.715 kb on - strand, within MMJJ_RS08685 at 1597.718 kb on + strand, within MMJJ_RS08685 at 1597.721 kb on + strand, within MMJJ_RS08685 at 1597.740 kb on + strand, within MMJJ_RS08685 at 1597.790 kb on - strand, within MMJJ_RS08685 at 1597.835 kb on + strand, within MMJJ_RS08685 at 1597.901 kb on + strand, within MMJJ_RS08685 at 1597.919 kb on + strand, within MMJJ_RS08685 at 1597.919 kb on + strand, within MMJJ_RS08685 at 1597.919 kb on + strand, within MMJJ_RS08685 at 1597.929 kb on + strand, within MMJJ_RS08685 at 1597.972 kb on - strand, within MMJJ_RS08685 at 1597.973 kb on + strand, within MMJJ_RS08685 at 1597.985 kb on - strand, within MMJJ_RS08685 at 1597.985 kb on - strand, within MMJJ_RS08685 at 1597.991 kb on - strand, within MMJJ_RS08685 at 1598.025 kb on - strand, within MMJJ_RS08685 at 1598.058 kb on + strand, within MMJJ_RS08685 at 1598.070 kb on + strand, within MMJJ_RS08685 at 1598.070 kb on + strand, within MMJJ_RS08685 at 1598.077 kb on - strand, within MMJJ_RS08685 at 1598.078 kb on + strand, within MMJJ_RS08685 at 1598.078 kb on + strand, within MMJJ_RS08685 at 1598.091 kb on - strand, within MMJJ_RS08685 at 1598.091 kb on - strand, within MMJJ_RS08685 at 1598.091 kb on - strand, within MMJJ_RS08685 at 1598.096 kb on - strand, within MMJJ_RS08685 at 1598.096 kb on - strand, within MMJJ_RS08685 at 1598.096 kb on - strand, within MMJJ_RS08685 at 1598.135 kb on - strand, within MMJJ_RS08685 at 1598.136 kb on + strand, within MMJJ_RS08685 at 1598.136 kb on + strand, within MMJJ_RS08685 at 1598.138 kb on - strand, within MMJJ_RS08685 at 1598.141 kb on + strand, within MMJJ_RS08685 at 1598.196 kb on + strand, within MMJJ_RS08685 at 1598.211 kb on + strand, within MMJJ_RS08685 at 1598.223 kb on + strand, within MMJJ_RS08685 at 1598.228 kb on - strand, within MMJJ_RS08685 at 1598.331 kb on + strand, within MMJJ_RS08685 at 1598.345 kb on + strand, within MMJJ_RS08685 at 1598.349 kb on + strand, within MMJJ_RS08685 at 1598.374 kb on - strand at 1598.375 kb on + strand at 1598.378 kb on - strand at 1598.379 kb on + strand at 1598.387 kb on - strand at 1598.405 kb on - strand at 1598.408 kb on - strand at 1598.410 kb on - strand at 1598.411 kb on + strand at 1598.411 kb on + strand at 1598.444 kb on + strand at 1598.463 kb on + strand at 1598.463 kb on + strand at 1598.469 kb on + strand at 1598.469 kb on + strand at 1598.519 kb on - strand at 1598.537 kb on + strand at 1598.537 kb on + strand at 1598.546 kb on - strand at 1598.547 kb on + strand at 1598.584 kb on - strand at 1598.599 kb on + strand at 1598.634 kb on - strand, within MMJJ_RS08690 at 1598.638 kb on + strand, within MMJJ_RS08690 at 1598.644 kb on - strand, within MMJJ_RS08690 at 1598.644 kb on - strand, within MMJJ_RS08690 at 1598.644 kb on - strand, within MMJJ_RS08690 at 1598.645 kb on + strand, within MMJJ_RS08690 at 1598.645 kb on + strand, within MMJJ_RS08690 at 1598.645 kb on - strand, within MMJJ_RS08690 at 1598.663 kb on - strand, within MMJJ_RS08690 at 1598.663 kb on - strand, within MMJJ_RS08690 at 1598.664 kb on + strand, within MMJJ_RS08690 at 1598.664 kb on + strand, within MMJJ_RS08690 at 1598.664 kb on + strand, within MMJJ_RS08690 at 1598.664 kb on + strand, within MMJJ_RS08690 at 1598.664 kb on + strand, within MMJJ_RS08690 at 1598.664 kb on + strand, within MMJJ_RS08690 at 1598.664 kb on + strand, within MMJJ_RS08690 at 1598.678 kb on + strand, within MMJJ_RS08690 at 1598.692 kb on + strand, within MMJJ_RS08690 at 1598.699 kb on + strand, within MMJJ_RS08690 at 1598.721 kb on - strand, within MMJJ_RS08690 at 1598.722 kb on + strand, within MMJJ_RS08690 at 1598.738 kb on - strand, within MMJJ_RS08690 at 1598.739 kb on + strand, within MMJJ_RS08690 at 1598.905 kb on + strand, within MMJJ_RS08690 at 1598.905 kb on + strand, within MMJJ_RS08690 at 1599.014 kb on + strand, within MMJJ_RS08690 at 1599.280 kb on + strand, within MMJJ_RS08690 at 1599.292 kb on + strand at 1599.294 kb on - strand at 1599.295 kb on + strand at 1599.295 kb on + strand at 1599.295 kb on + strand at 1599.350 kb on + strand at 1599.350 kb on + strand at 1599.358 kb on - strand at 1599.358 kb on - strand at 1599.358 kb on - strand at 1599.359 kb on + strand at 1599.359 kb on + strand at 1599.359 kb on + strand at 1599.359 kb on + strand at 1599.359 kb on + strand at 1599.361 kb on + strand at 1599.361 kb on + strand at 1599.361 kb on + strand at 1599.392 kb on - strand at 1599.397 kb on - strand at 1599.398 kb on + strand at 1599.399 kb on - strand at 1599.399 kb on - strand at 1599.400 kb on + strand at 1599.474 kb on + strand at 1599.474 kb on + strand at 1599.474 kb on + strand at 1599.486 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Spiramycin 0.1 mM remove 1,597,555 - +0.3 1,597,556 + +1.5 1,597,607 + -0.7 1,597,607 + +0.4 1,597,609 + +2.0 1,597,617 + -2.0 1,597,620 + -1.5 1,597,620 + -1.5 1,597,640 - +0.6 1,597,650 + -0.2 1,597,675 + MMJJ_RS08685 0.12 +1.1 1,597,708 - MMJJ_RS08685 0.15 +1.0 1,597,715 - MMJJ_RS08685 0.16 -0.7 1,597,718 + MMJJ_RS08685 0.16 -1.6 1,597,721 + MMJJ_RS08685 0.17 -0.6 1,597,740 + MMJJ_RS08685 0.19 +0.9 1,597,790 - MMJJ_RS08685 0.25 +1.1 1,597,835 + MMJJ_RS08685 0.30 +1.0 1,597,901 + MMJJ_RS08685 0.37 -1.7 1,597,919 + MMJJ_RS08685 0.39 +1.4 1,597,919 + MMJJ_RS08685 0.39 +0.6 1,597,919 + MMJJ_RS08685 0.39 -0.7 1,597,929 + MMJJ_RS08685 0.41 +0.7 1,597,972 - MMJJ_RS08685 0.45 +0.8 1,597,973 + MMJJ_RS08685 0.46 -1.1 1,597,985 - MMJJ_RS08685 0.47 +0.5 1,597,985 - MMJJ_RS08685 0.47 +0.1 1,597,991 - MMJJ_RS08685 0.48 +1.0 1,598,025 - MMJJ_RS08685 0.51 +1.2 1,598,058 + MMJJ_RS08685 0.55 +1.5 1,598,070 + MMJJ_RS08685 0.57 +1.6 1,598,070 + MMJJ_RS08685 0.57 +0.2 1,598,077 - MMJJ_RS08685 0.57 +1.5 1,598,078 + MMJJ_RS08685 0.58 -0.4 1,598,078 + MMJJ_RS08685 0.58 -1.3 1,598,091 - MMJJ_RS08685 0.59 +0.8 1,598,091 - MMJJ_RS08685 0.59 +0.5 1,598,091 - MMJJ_RS08685 0.59 -1.3 1,598,096 - MMJJ_RS08685 0.60 -0.5 1,598,096 - MMJJ_RS08685 0.60 +1.0 1,598,096 - MMJJ_RS08685 0.60 +1.2 1,598,135 - MMJJ_RS08685 0.64 +2.9 1,598,136 + MMJJ_RS08685 0.64 -3.7 1,598,136 + MMJJ_RS08685 0.64 -2.7 1,598,138 - MMJJ_RS08685 0.64 +1.6 1,598,141 + MMJJ_RS08685 0.65 -1.5 1,598,196 + MMJJ_RS08685 0.71 +0.4 1,598,211 + MMJJ_RS08685 0.73 -0.1 1,598,223 + MMJJ_RS08685 0.74 +2.0 1,598,228 - MMJJ_RS08685 0.75 +0.5 1,598,331 + MMJJ_RS08685 0.86 +1.6 1,598,345 + MMJJ_RS08685 0.88 +0.9 1,598,349 + MMJJ_RS08685 0.88 +1.7 1,598,374 - +0.9 1,598,375 + -3.0 1,598,378 - +0.6 1,598,379 + -1.5 1,598,387 - +2.6 1,598,405 - -0.9 1,598,408 - +0.4 1,598,410 - +0.3 1,598,411 + +1.4 1,598,411 + +0.4 1,598,444 + -1.5 1,598,463 + +1.1 1,598,463 + -0.7 1,598,469 + -1.5 1,598,469 + -0.1 1,598,519 - -1.2 1,598,537 + +1.4 1,598,537 + -0.6 1,598,546 - +1.1 1,598,547 + +0.2 1,598,584 - +2.0 1,598,599 + -0.1 1,598,634 - MMJJ_RS08690 0.12 -1.1 1,598,638 + MMJJ_RS08690 0.13 +1.5 1,598,644 - MMJJ_RS08690 0.14 +0.8 1,598,644 - MMJJ_RS08690 0.14 +0.9 1,598,644 - MMJJ_RS08690 0.14 -1.0 1,598,645 + MMJJ_RS08690 0.14 -0.1 1,598,645 + MMJJ_RS08690 0.14 +1.5 1,598,645 - MMJJ_RS08690 0.14 +0.6 1,598,663 - MMJJ_RS08690 0.16 +0.1 1,598,663 - MMJJ_RS08690 0.16 +0.0 1,598,664 + MMJJ_RS08690 0.16 +0.7 1,598,664 + MMJJ_RS08690 0.16 -0.9 1,598,664 + MMJJ_RS08690 0.16 -0.8 1,598,664 + MMJJ_RS08690 0.16 -1.3 1,598,664 + MMJJ_RS08690 0.16 -0.2 1,598,664 + MMJJ_RS08690 0.16 -0.9 1,598,664 + MMJJ_RS08690 0.16 -2.5 1,598,678 + MMJJ_RS08690 0.18 +2.1 1,598,692 + MMJJ_RS08690 0.19 -1.1 1,598,699 + MMJJ_RS08690 0.20 -3.2 1,598,721 - MMJJ_RS08690 0.23 -2.3 1,598,722 + MMJJ_RS08690 0.23 -1.3 1,598,738 - MMJJ_RS08690 0.25 -1.1 1,598,739 + MMJJ_RS08690 0.25 -1.4 1,598,905 + MMJJ_RS08690 0.44 +1.3 1,598,905 + MMJJ_RS08690 0.44 +0.0 1,599,014 + MMJJ_RS08690 0.57 -0.8 1,599,280 + MMJJ_RS08690 0.89 -1.3 1,599,292 + +1.7 1,599,294 - +1.2 1,599,295 + -0.5 1,599,295 + +0.7 1,599,295 + +1.9 1,599,350 + -2.7 1,599,350 + -3.0 1,599,358 - -0.7 1,599,358 - -1.6 1,599,358 - -0.1 1,599,359 + -0.5 1,599,359 + -0.1 1,599,359 + +0.6 1,599,359 + -0.2 1,599,359 + -1.8 1,599,361 + +0.0 1,599,361 + +1.5 1,599,361 + +0.5 1,599,392 - -0.6 1,599,397 - -0.3 1,599,398 + -2.2 1,599,399 - -0.1 1,599,399 - -1.2 1,599,400 + +0.9 1,599,474 + -0.9 1,599,474 + +0.4 1,599,474 + -3.1 1,599,486 - -1.3
Or see this region's nucleotide sequence