Experiment: McCas_formate_complete Spiramycin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04700 and MMJJ_RS04705 are separated by 56 nucleotides MMJJ_RS04705 and MMJJ_RS04710 are separated by 17 nucleotides MMJJ_RS04710 and MMJJ_RS04715 are separated by 271 nucleotides MMJJ_RS04715 and MMJJ_RS04720 are separated by 49 nucleotides
MMJJ_RS04700: MMJJ_RS04700 - deoxyhypusine synthase, at 881,208 to 882,215
_RS04700
MMJJ_RS04705: MMJJ_RS04705 - 3-isopropylmalate dehydratase small subunit, at 882,272 to 882,775
_RS04705
MMJJ_RS04710: MMJJ_RS04710 - threonine synthase, at 882,793 to 884,007
_RS04710
MMJJ_RS04715: MMJJ_RS04715 - hypothetical protein, at 884,279 to 884,623
_RS04715
MMJJ_RS04720: MMJJ_RS04720 - IMP dehydrogenase, at 884,673 to 886,175
_RS04720
Position (kb) 882
883
884
885 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 881.795 kb on + strand, within MMJJ_RS04700 at 881.795 kb on + strand, within MMJJ_RS04700 at 881.797 kb on + strand, within MMJJ_RS04700 at 881.912 kb on - strand, within MMJJ_RS04700 at 881.944 kb on + strand, within MMJJ_RS04700 at 881.955 kb on - strand, within MMJJ_RS04700 at 881.996 kb on + strand, within MMJJ_RS04700 at 882.273 kb on - strand at 882.710 kb on - strand, within MMJJ_RS04705 at 884.062 kb on - strand at 884.063 kb on + strand at 884.089 kb on + strand at 884.089 kb on + strand at 884.091 kb on - strand at 884.151 kb on - strand at 884.157 kb on - strand at 884.177 kb on - strand at 884.177 kb on - strand at 884.182 kb on + strand at 884.182 kb on + strand at 884.184 kb on - strand at 884.184 kb on - strand at 884.188 kb on - strand at 884.188 kb on - strand at 884.188 kb on - strand at 884.188 kb on - strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.190 kb on - strand at 884.218 kb on - strand at 884.218 kb on - strand at 884.218 kb on - strand at 884.218 kb on - strand at 884.218 kb on - strand at 884.222 kb on - strand at 884.222 kb on - strand at 884.222 kb on - strand at 884.222 kb on - strand at 884.239 kb on + strand at 884.280 kb on - strand at 884.287 kb on + strand at 884.300 kb on - strand at 884.300 kb on - strand at 884.300 kb on - strand at 884.300 kb on - strand at 884.303 kb on + strand at 884.325 kb on + strand, within MMJJ_RS04715 at 884.372 kb on - strand, within MMJJ_RS04715 at 884.425 kb on - strand, within MMJJ_RS04715 at 884.425 kb on - strand, within MMJJ_RS04715 at 884.425 kb on - strand, within MMJJ_RS04715 at 884.426 kb on + strand, within MMJJ_RS04715 at 884.426 kb on + strand, within MMJJ_RS04715 at 884.426 kb on + strand, within MMJJ_RS04715 at 884.481 kb on + strand, within MMJJ_RS04715 at 884.497 kb on - strand, within MMJJ_RS04715 at 884.497 kb on - strand, within MMJJ_RS04715 at 884.498 kb on + strand, within MMJJ_RS04715 at 884.498 kb on + strand, within MMJJ_RS04715 at 884.609 kb on + strand at 884.609 kb on + strand at 884.639 kb on + strand at 884.639 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Spiramycin 0.1 mM remove 881,795 + MMJJ_RS04700 0.58 -1.9 881,795 + MMJJ_RS04700 0.58 -1.9 881,797 + MMJJ_RS04700 0.58 -0.9 881,912 - MMJJ_RS04700 0.70 -1.9 881,944 + MMJJ_RS04700 0.73 +1.1 881,955 - MMJJ_RS04700 0.74 -1.7 881,996 + MMJJ_RS04700 0.78 +0.7 882,273 - +1.1 882,710 - MMJJ_RS04705 0.87 -2.1 884,062 - +3.0 884,063 + -0.9 884,089 + +1.6 884,089 + +1.0 884,091 - -1.6 884,151 - +0.1 884,157 - +0.6 884,177 - -0.9 884,177 - -0.7 884,182 + +0.8 884,182 + -1.7 884,184 - -0.6 884,184 - +0.4 884,188 - -1.8 884,188 - +2.5 884,188 - +1.8 884,188 - +0.7 884,189 + -0.3 884,189 + -0.0 884,189 + -1.5 884,189 + -0.0 884,189 + -0.3 884,189 + +0.3 884,189 + +2.1 884,189 + +1.0 884,189 + -2.0 884,189 + -3.9 884,189 + +0.2 884,190 - +1.2 884,218 - +2.2 884,218 - -1.2 884,218 - +0.7 884,218 - -1.3 884,218 - +0.9 884,222 - -0.5 884,222 - -0.9 884,222 - -0.1 884,222 - +1.2 884,239 + -0.4 884,280 - +0.3 884,287 + +2.0 884,300 - +2.3 884,300 - +1.5 884,300 - -1.9 884,300 - +0.1 884,303 + -0.7 884,325 + MMJJ_RS04715 0.13 +1.6 884,372 - MMJJ_RS04715 0.27 -0.6 884,425 - MMJJ_RS04715 0.42 -2.1 884,425 - MMJJ_RS04715 0.42 -1.3 884,425 - MMJJ_RS04715 0.42 +0.5 884,426 + MMJJ_RS04715 0.43 +1.0 884,426 + MMJJ_RS04715 0.43 -1.3 884,426 + MMJJ_RS04715 0.43 +1.5 884,481 + MMJJ_RS04715 0.59 +0.2 884,497 - MMJJ_RS04715 0.63 -0.1 884,497 - MMJJ_RS04715 0.63 -1.3 884,498 + MMJJ_RS04715 0.63 -2.7 884,498 + MMJJ_RS04715 0.63 -0.5 884,609 + -0.3 884,609 + -1.0 884,639 + -1.3 884,639 + -2.3
Or see this region's nucleotide sequence