Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_21230 and IAI47_21235 are separated by 112 nucleotides IAI47_21235 and IAI47_21240 are separated by 382 nucleotides IAI47_21240 and IAI47_21245 are separated by 240 nucleotides
IAI47_21230: IAI47_21230 - DUF4142 domain-containing protein, at 504,221 to 504,790
_21230
IAI47_21235: IAI47_21235 - hypothetical protein, at 504,903 to 505,061
_21235
IAI47_21240: IAI47_21240 - hypothetical protein, at 505,444 to 505,953
_21240
IAI47_21245: IAI47_21245 - alcohol dehydrogenase catalytic domain-containing protein, at 506,194 to 507,273
_21245
Position (kb) 505
506 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 504.454 kb on + strand, within IAI47_21230 at 504.454 kb on + strand, within IAI47_21230 at 504.454 kb on + strand, within IAI47_21230 at 504.455 kb on - strand, within IAI47_21230 at 504.455 kb on - strand, within IAI47_21230 at 504.458 kb on + strand, within IAI47_21230 at 504.458 kb on + strand, within IAI47_21230 at 504.458 kb on + strand, within IAI47_21230 at 504.459 kb on - strand, within IAI47_21230 at 504.459 kb on - strand, within IAI47_21230 at 504.478 kb on + strand, within IAI47_21230 at 504.544 kb on + strand, within IAI47_21230 at 504.602 kb on - strand, within IAI47_21230 at 504.662 kb on - strand, within IAI47_21230 at 504.662 kb on - strand, within IAI47_21230 at 504.662 kb on - strand, within IAI47_21230 at 504.676 kb on + strand, within IAI47_21230 at 504.676 kb on + strand, within IAI47_21230 at 504.676 kb on + strand, within IAI47_21230 at 504.676 kb on + strand, within IAI47_21230 at 504.677 kb on - strand, within IAI47_21230 at 504.752 kb on + strand at 504.753 kb on - strand at 504.770 kb on - strand at 504.771 kb on + strand at 504.778 kb on + strand at 504.809 kb on - strand at 504.809 kb on - strand at 504.847 kb on + strand at 504.855 kb on + strand at 504.856 kb on - strand at 504.865 kb on - strand at 504.894 kb on + strand at 504.894 kb on + strand at 504.927 kb on + strand, within IAI47_21235 at 504.928 kb on - strand, within IAI47_21235 at 504.944 kb on - strand, within IAI47_21235 at 505.034 kb on + strand, within IAI47_21235 at 505.068 kb on + strand at 505.087 kb on + strand at 505.087 kb on + strand at 505.088 kb on - strand at 505.088 kb on - strand at 505.088 kb on - strand at 505.089 kb on + strand at 505.089 kb on + strand at 505.090 kb on - strand at 505.090 kb on - strand at 505.090 kb on - strand at 505.090 kb on - strand at 505.090 kb on - strand at 505.101 kb on - strand at 505.103 kb on + strand at 505.103 kb on + strand at 505.112 kb on + strand at 505.115 kb on + strand at 505.137 kb on + strand at 505.137 kb on + strand at 505.138 kb on - strand at 505.139 kb on + strand at 505.140 kb on - strand at 505.140 kb on - strand at 505.160 kb on - strand at 505.191 kb on - strand at 505.205 kb on - strand at 505.209 kb on - strand at 505.332 kb on + strand at 505.354 kb on + strand at 505.355 kb on - strand at 505.387 kb on + strand at 505.387 kb on + strand at 505.387 kb on + strand at 505.388 kb on - strand at 505.399 kb on + strand at 505.424 kb on + strand at 505.428 kb on - strand at 505.428 kb on - strand at 505.430 kb on + strand at 505.441 kb on - strand at 505.441 kb on - strand at 505.485 kb on + strand at 505.485 kb on + strand at 505.485 kb on + strand at 505.486 kb on - strand at 505.488 kb on - strand at 505.488 kb on - strand at 505.563 kb on + strand, within IAI47_21240 at 505.563 kb on + strand, within IAI47_21240 at 505.563 kb on + strand, within IAI47_21240 at 505.564 kb on - strand, within IAI47_21240 at 505.566 kb on + strand, within IAI47_21240 at 505.567 kb on - strand, within IAI47_21240 at 505.581 kb on + strand, within IAI47_21240 at 505.581 kb on + strand, within IAI47_21240 at 505.584 kb on + strand, within IAI47_21240 at 505.585 kb on - strand, within IAI47_21240 at 505.656 kb on + strand, within IAI47_21240 at 505.657 kb on - strand, within IAI47_21240 at 505.692 kb on + strand, within IAI47_21240 at 505.693 kb on - strand, within IAI47_21240 at 505.693 kb on - strand, within IAI47_21240 at 505.693 kb on - strand, within IAI47_21240 at 505.693 kb on - strand, within IAI47_21240 at 505.699 kb on + strand, within IAI47_21240 at 505.700 kb on - strand, within IAI47_21240 at 505.713 kb on + strand, within IAI47_21240 at 505.713 kb on + strand, within IAI47_21240 at 505.729 kb on + strand, within IAI47_21240 at 505.767 kb on - strand, within IAI47_21240 at 505.838 kb on + strand, within IAI47_21240 at 505.838 kb on + strand, within IAI47_21240 at 505.839 kb on - strand, within IAI47_21240 at 505.839 kb on - strand, within IAI47_21240 at 505.852 kb on + strand, within IAI47_21240 at 505.853 kb on - strand, within IAI47_21240 at 505.855 kb on + strand, within IAI47_21240 at 505.864 kb on + strand, within IAI47_21240 at 505.865 kb on - strand, within IAI47_21240 at 505.881 kb on - strand, within IAI47_21240 at 505.899 kb on + strand, within IAI47_21240 at 505.899 kb on + strand, within IAI47_21240 at 505.899 kb on + strand, within IAI47_21240 at 505.899 kb on + strand, within IAI47_21240 at 505.900 kb on - strand, within IAI47_21240 at 505.900 kb on - strand, within IAI47_21240 at 505.907 kb on + strand at 505.922 kb on + strand at 505.962 kb on + strand at 505.975 kb on + strand at 505.975 kb on + strand at 505.979 kb on + strand at 505.979 kb on + strand at 505.979 kb on + strand at 505.980 kb on - strand at 505.980 kb on - strand at 505.980 kb on - strand at 505.980 kb on - strand at 506.007 kb on + strand at 506.008 kb on - strand at 506.008 kb on - strand at 506.008 kb on - strand at 506.013 kb on + strand at 506.014 kb on - strand at 506.017 kb on + strand at 506.017 kb on + strand at 506.018 kb on - strand at 506.040 kb on + strand at 506.040 kb on + strand at 506.040 kb on + strand at 506.040 kb on + strand at 506.041 kb on - strand at 506.042 kb on + strand at 506.042 kb on + strand at 506.043 kb on - strand at 506.051 kb on + strand at 506.052 kb on - strand at 506.052 kb on - strand at 506.093 kb on - strand at 506.110 kb on + strand at 506.111 kb on - strand at 506.119 kb on - strand at 506.136 kb on + strand at 506.137 kb on - strand at 506.137 kb on - strand at 506.153 kb on + strand at 506.168 kb on + strand at 506.168 kb on + strand at 506.217 kb on + strand at 506.260 kb on - strand at 506.260 kb on - strand at 506.260 kb on - strand at 506.263 kb on - strand at 506.292 kb on - strand at 506.308 kb on + strand, within IAI47_21245 at 506.339 kb on + strand, within IAI47_21245 at 506.339 kb on + strand, within IAI47_21245 at 506.358 kb on - strand, within IAI47_21245 at 506.358 kb on - strand, within IAI47_21245 at 506.358 kb on - strand, within IAI47_21245 at 506.373 kb on + strand, within IAI47_21245 at 506.373 kb on + strand, within IAI47_21245 at 506.373 kb on + strand, within IAI47_21245 at 506.374 kb on - strand, within IAI47_21245 at 506.374 kb on - strand, within IAI47_21245 at 506.374 kb on - strand, within IAI47_21245 at 506.406 kb on - strand, within IAI47_21245 at 506.468 kb on + strand, within IAI47_21245 at 506.468 kb on + strand, within IAI47_21245 at 506.484 kb on + strand, within IAI47_21245 at 506.484 kb on + strand, within IAI47_21245 at 506.485 kb on - strand, within IAI47_21245 at 506.580 kb on + strand, within IAI47_21245 at 506.580 kb on + strand, within IAI47_21245 at 506.580 kb on + strand, within IAI47_21245 at 506.581 kb on - strand, within IAI47_21245 at 506.685 kb on + strand, within IAI47_21245 at 506.686 kb on - strand, within IAI47_21245 at 506.686 kb on - strand, within IAI47_21245 at 506.755 kb on + strand, within IAI47_21245 at 506.756 kb on - strand, within IAI47_21245 at 506.788 kb on + strand, within IAI47_21245 at 506.788 kb on + strand, within IAI47_21245 at 506.788 kb on + strand, within IAI47_21245 at 506.789 kb on - strand, within IAI47_21245 at 506.798 kb on + strand, within IAI47_21245 at 506.799 kb on - strand, within IAI47_21245 at 506.799 kb on - strand, within IAI47_21245 at 506.799 kb on - strand, within IAI47_21245 at 506.799 kb on - strand, within IAI47_21245 at 506.799 kb on - strand, within IAI47_21245 at 506.814 kb on + strand, within IAI47_21245 at 506.814 kb on + strand, within IAI47_21245 at 506.814 kb on + strand, within IAI47_21245 at 506.814 kb on + strand, within IAI47_21245 at 506.815 kb on - strand, within IAI47_21245 at 506.815 kb on - strand, within IAI47_21245 at 506.815 kb on - strand, within IAI47_21245 at 506.815 kb on - strand, within IAI47_21245 at 506.921 kb on + strand, within IAI47_21245 at 506.921 kb on + strand, within IAI47_21245 at 506.921 kb on + strand, within IAI47_21245 at 506.921 kb on + strand, within IAI47_21245 at 506.921 kb on + strand, within IAI47_21245 at 506.922 kb on - strand, within IAI47_21245 at 506.922 kb on - strand, within IAI47_21245 at 506.922 kb on - strand, within IAI47_21245 at 506.922 kb on - strand, within IAI47_21245
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 504,454 + IAI47_21230 0.41 +2.2 504,454 + IAI47_21230 0.41 -1.3 504,454 + IAI47_21230 0.41 -0.6 504,455 - IAI47_21230 0.41 -0.3 504,455 - IAI47_21230 0.41 +0.0 504,458 + IAI47_21230 0.42 +1.7 504,458 + IAI47_21230 0.42 -2.4 504,458 + IAI47_21230 0.42 +1.1 504,459 - IAI47_21230 0.42 +1.4 504,459 - IAI47_21230 0.42 -0.1 504,478 + IAI47_21230 0.45 -0.6 504,544 + IAI47_21230 0.57 +1.0 504,602 - IAI47_21230 0.67 -1.1 504,662 - IAI47_21230 0.77 -0.3 504,662 - IAI47_21230 0.77 -0.2 504,662 - IAI47_21230 0.77 -1.5 504,676 + IAI47_21230 0.80 +0.5 504,676 + IAI47_21230 0.80 +0.9 504,676 + IAI47_21230 0.80 -0.3 504,676 + IAI47_21230 0.80 -0.2 504,677 - IAI47_21230 0.80 +1.0 504,752 + +1.2 504,753 - +1.8 504,770 - +2.8 504,771 + +0.6 504,778 + -0.1 504,809 - +0.6 504,809 - -0.2 504,847 + -0.4 504,855 + +0.2 504,856 - -0.0 504,865 - +2.2 504,894 + +0.5 504,894 + -0.8 504,927 + IAI47_21235 0.15 -0.7 504,928 - IAI47_21235 0.16 +0.1 504,944 - IAI47_21235 0.26 +1.1 505,034 + IAI47_21235 0.82 -1.5 505,068 + +0.5 505,087 + -0.1 505,087 + -0.5 505,088 - +0.1 505,088 - +0.9 505,088 - -1.8 505,089 + -1.5 505,089 + -1.3 505,090 - +2.5 505,090 - -1.2 505,090 - -0.6 505,090 - -2.1 505,090 - +1.0 505,101 - +0.4 505,103 + -0.8 505,103 + -2.3 505,112 + -0.9 505,115 + -0.6 505,137 + -0.2 505,137 + -2.4 505,138 - -0.2 505,139 + -3.2 505,140 - -1.0 505,140 - -0.1 505,160 - -1.5 505,191 - +2.5 505,205 - +0.0 505,209 - +0.8 505,332 + +0.3 505,354 + -1.7 505,355 - -0.9 505,387 + -1.0 505,387 + +1.2 505,387 + +0.2 505,388 - +1.5 505,399 + -0.2 505,424 + -0.4 505,428 - -0.4 505,428 - +0.4 505,430 + +1.0 505,441 - +0.7 505,441 - -2.0 505,485 + +0.5 505,485 + +0.7 505,485 + -0.0 505,486 - -1.1 505,488 - +1.9 505,488 - +0.5 505,563 + IAI47_21240 0.23 +0.1 505,563 + IAI47_21240 0.23 +1.5 505,563 + IAI47_21240 0.23 +0.3 505,564 - IAI47_21240 0.24 +1.2 505,566 + IAI47_21240 0.24 +2.2 505,567 - IAI47_21240 0.24 +1.1 505,581 + IAI47_21240 0.27 +2.2 505,581 + IAI47_21240 0.27 +0.7 505,584 + IAI47_21240 0.27 +1.1 505,585 - IAI47_21240 0.28 +0.7 505,656 + IAI47_21240 0.42 -0.4 505,657 - IAI47_21240 0.42 -0.9 505,692 + IAI47_21240 0.49 -0.8 505,693 - IAI47_21240 0.49 -0.5 505,693 - IAI47_21240 0.49 +1.2 505,693 - IAI47_21240 0.49 -1.5 505,693 - IAI47_21240 0.49 -1.1 505,699 + IAI47_21240 0.50 +0.9 505,700 - IAI47_21240 0.50 -1.3 505,713 + IAI47_21240 0.53 +0.7 505,713 + IAI47_21240 0.53 -0.5 505,729 + IAI47_21240 0.56 +1.2 505,767 - IAI47_21240 0.63 +0.4 505,838 + IAI47_21240 0.77 +0.2 505,838 + IAI47_21240 0.77 -1.7 505,839 - IAI47_21240 0.77 +1.2 505,839 - IAI47_21240 0.77 +1.2 505,852 + IAI47_21240 0.80 +0.2 505,853 - IAI47_21240 0.80 -0.2 505,855 + IAI47_21240 0.81 +0.5 505,864 + IAI47_21240 0.82 -0.6 505,865 - IAI47_21240 0.83 +1.0 505,881 - IAI47_21240 0.86 +2.8 505,899 + IAI47_21240 0.89 +1.1 505,899 + IAI47_21240 0.89 +1.6 505,899 + IAI47_21240 0.89 +0.9 505,899 + IAI47_21240 0.89 +1.9 505,900 - IAI47_21240 0.89 -0.6 505,900 - IAI47_21240 0.89 +1.5 505,907 + -1.8 505,922 + -0.2 505,962 + -0.6 505,975 + +0.2 505,975 + +0.8 505,979 + +2.0 505,979 + +0.9 505,979 + -0.6 505,980 - +0.3 505,980 - -0.3 505,980 - -0.3 505,980 - +0.7 506,007 + -0.1 506,008 - -1.8 506,008 - +0.4 506,008 - +1.6 506,013 + +1.5 506,014 - +0.0 506,017 + -0.2 506,017 + -1.2 506,018 - -1.1 506,040 + -1.4 506,040 + +1.2 506,040 + -0.2 506,040 + -0.9 506,041 - -0.5 506,042 + +0.7 506,042 + -1.1 506,043 - +1.8 506,051 + -0.4 506,052 - +1.1 506,052 - +0.2 506,093 - +0.3 506,110 + +0.6 506,111 - +0.7 506,119 - -2.8 506,136 + -1.3 506,137 - +0.7 506,137 - -1.4 506,153 + -1.0 506,168 + -0.5 506,168 + +0.8 506,217 + +1.3 506,260 - +1.8 506,260 - +1.4 506,260 - +1.4 506,263 - -0.2 506,292 - +3.1 506,308 + IAI47_21245 0.11 +0.5 506,339 + IAI47_21245 0.13 -2.3 506,339 + IAI47_21245 0.13 +1.6 506,358 - IAI47_21245 0.15 -1.1 506,358 - IAI47_21245 0.15 -0.7 506,358 - IAI47_21245 0.15 +0.0 506,373 + IAI47_21245 0.17 +1.1 506,373 + IAI47_21245 0.17 -0.3 506,373 + IAI47_21245 0.17 -0.9 506,374 - IAI47_21245 0.17 -2.0 506,374 - IAI47_21245 0.17 -1.3 506,374 - IAI47_21245 0.17 +0.7 506,406 - IAI47_21245 0.20 -2.2 506,468 + IAI47_21245 0.25 -0.5 506,468 + IAI47_21245 0.25 +1.4 506,484 + IAI47_21245 0.27 +0.2 506,484 + IAI47_21245 0.27 +1.1 506,485 - IAI47_21245 0.27 +0.1 506,580 + IAI47_21245 0.36 -1.9 506,580 + IAI47_21245 0.36 +0.7 506,580 + IAI47_21245 0.36 -0.3 506,581 - IAI47_21245 0.36 +0.5 506,685 + IAI47_21245 0.45 -1.4 506,686 - IAI47_21245 0.46 +2.7 506,686 - IAI47_21245 0.46 -0.2 506,755 + IAI47_21245 0.52 +1.0 506,756 - IAI47_21245 0.52 -0.3 506,788 + IAI47_21245 0.55 +0.9 506,788 + IAI47_21245 0.55 +0.6 506,788 + IAI47_21245 0.55 +0.4 506,789 - IAI47_21245 0.55 -0.3 506,798 + IAI47_21245 0.56 +0.6 506,799 - IAI47_21245 0.56 -1.7 506,799 - IAI47_21245 0.56 -0.4 506,799 - IAI47_21245 0.56 +0.0 506,799 - IAI47_21245 0.56 -1.8 506,799 - IAI47_21245 0.56 +0.1 506,814 + IAI47_21245 0.57 -0.2 506,814 + IAI47_21245 0.57 +1.7 506,814 + IAI47_21245 0.57 +1.3 506,814 + IAI47_21245 0.57 +0.1 506,815 - IAI47_21245 0.57 +1.1 506,815 - IAI47_21245 0.57 -0.8 506,815 - IAI47_21245 0.57 +1.4 506,815 - IAI47_21245 0.57 -0.2 506,921 + IAI47_21245 0.67 +0.4 506,921 + IAI47_21245 0.67 -1.5 506,921 + IAI47_21245 0.67 -0.1 506,921 + IAI47_21245 0.67 +0.8 506,921 + IAI47_21245 0.67 -0.2 506,922 - IAI47_21245 0.67 -1.3 506,922 - IAI47_21245 0.67 +0.1 506,922 - IAI47_21245 0.67 +0.9 506,922 - IAI47_21245 0.67 -0.5
Or see this region's nucleotide sequence