Experiment: McCas_formate_complete sodium nitrite 0.125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS09020 and MMJJ_RS09025 are separated by 71 nucleotides MMJJ_RS09025 and MMJJ_RS09030 are separated by 21 nucleotides MMJJ_RS09030 and MMJJ_RS09035 are separated by 162 nucleotides
MMJJ_RS09020: MMJJ_RS09020 - hypothetical protein, at 1,664,223 to 1,664,636
_RS09020
MMJJ_RS09025: MMJJ_RS09025 - dTMP kinase, at 1,664,708 to 1,665,307
_RS09025
MMJJ_RS09030: MMJJ_RS09030 - hypothetical protein, at 1,665,329 to 1,668,259
_RS09030
MMJJ_RS09035: MMJJ_RS09035 - OB-fold nucleic acid binding domain-containing protein, at 1,668,422 to 1,670,350
_RS09035
Position (kb)
1665
1666
1667
1668
1669 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 1664.334 kb on - strand, within MMJJ_RS09020 at 1664.338 kb on + strand, within MMJJ_RS09020 at 1664.458 kb on - strand, within MMJJ_RS09020 at 1664.459 kb on + strand, within MMJJ_RS09020 at 1664.459 kb on + strand, within MMJJ_RS09020 at 1664.459 kb on + strand, within MMJJ_RS09020 at 1664.470 kb on + strand, within MMJJ_RS09020 at 1664.482 kb on + strand, within MMJJ_RS09020 at 1664.530 kb on - strand, within MMJJ_RS09020 at 1664.564 kb on - strand, within MMJJ_RS09020 at 1664.610 kb on - strand at 1664.612 kb on - strand at 1664.613 kb on + strand at 1664.619 kb on - strand at 1664.620 kb on + strand at 1664.621 kb on - strand at 1664.622 kb on + strand at 1664.622 kb on + strand at 1664.623 kb on - strand at 1664.623 kb on - strand at 1664.628 kb on - strand at 1664.628 kb on - strand at 1664.628 kb on - strand at 1664.629 kb on + strand at 1664.629 kb on + strand at 1664.629 kb on + strand at 1664.664 kb on + strand at 1664.664 kb on + strand at 1664.664 kb on + strand at 1664.664 kb on + strand at 1664.665 kb on - strand at 1664.665 kb on - strand at 1664.666 kb on + strand at 1664.667 kb on - strand at 1664.667 kb on - strand at 1664.668 kb on + strand at 1664.668 kb on + strand at 1664.668 kb on + strand at 1664.668 kb on + strand at 1664.675 kb on - strand at 1664.675 kb on - strand at 1664.675 kb on - strand at 1664.676 kb on + strand at 1664.679 kb on - strand at 1664.680 kb on + strand at 1664.691 kb on - strand at 1664.700 kb on - strand at 1664.701 kb on + strand at 1664.702 kb on - strand at 1664.703 kb on + strand at 1664.703 kb on + strand at 1664.704 kb on - strand at 1664.704 kb on - strand at 1664.705 kb on + strand at 1664.709 kb on - strand at 1664.714 kb on - strand at 1664.717 kb on + strand at 1664.719 kb on - strand at 1665.157 kb on - strand at 1665.327 kb on + strand at 1665.355 kb on + strand at 1665.389 kb on - strand at 1665.390 kb on + strand at 1665.433 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.481 kb on - strand at 1665.482 kb on + strand at 1665.494 kb on - strand at 1665.497 kb on - strand at 1665.501 kb on + strand at 1665.521 kb on - strand at 1665.587 kb on - strand at 1665.623 kb on - strand, within MMJJ_RS09030 at 1665.644 kb on - strand, within MMJJ_RS09030 at 1665.644 kb on - strand, within MMJJ_RS09030 at 1665.698 kb on - strand, within MMJJ_RS09030 at 1665.929 kb on + strand, within MMJJ_RS09030 at 1665.989 kb on - strand, within MMJJ_RS09030 at 1666.003 kb on - strand at 1666.027 kb on - strand, within MMJJ_RS09030 at 1666.107 kb on - strand, within MMJJ_RS09030 at 1666.112 kb on - strand, within MMJJ_RS09030 at 1666.227 kb on + strand, within MMJJ_RS09030 at 1666.229 kb on - strand, within MMJJ_RS09030 at 1666.298 kb on - strand, within MMJJ_RS09030 at 1666.309 kb on - strand, within MMJJ_RS09030 at 1666.309 kb on - strand, within MMJJ_RS09030 at 1666.380 kb on - strand, within MMJJ_RS09030 at 1666.434 kb on - strand, within MMJJ_RS09030 at 1666.435 kb on + strand, within MMJJ_RS09030 at 1666.461 kb on - strand, within MMJJ_RS09030 at 1666.527 kb on + strand, within MMJJ_RS09030 at 1666.536 kb on - strand, within MMJJ_RS09030 at 1666.536 kb on - strand, within MMJJ_RS09030 at 1666.536 kb on - strand, within MMJJ_RS09030 at 1666.541 kb on - strand, within MMJJ_RS09030 at 1666.541 kb on - strand, within MMJJ_RS09030 at 1666.542 kb on + strand, within MMJJ_RS09030 at 1666.542 kb on + strand, within MMJJ_RS09030 at 1666.543 kb on - strand, within MMJJ_RS09030 at 1666.561 kb on + strand, within MMJJ_RS09030 at 1666.574 kb on - strand, within MMJJ_RS09030 at 1666.578 kb on - strand, within MMJJ_RS09030 at 1666.616 kb on - strand, within MMJJ_RS09030 at 1666.617 kb on + strand, within MMJJ_RS09030 at 1666.694 kb on + strand, within MMJJ_RS09030 at 1666.695 kb on - strand, within MMJJ_RS09030 at 1666.695 kb on - strand, within MMJJ_RS09030 at 1666.781 kb on - strand, within MMJJ_RS09030 at 1666.980 kb on - strand, within MMJJ_RS09030 at 1666.981 kb on + strand, within MMJJ_RS09030 at 1666.981 kb on + strand, within MMJJ_RS09030 at 1667.125 kb on - strand, within MMJJ_RS09030 at 1667.127 kb on - strand, within MMJJ_RS09030 at 1667.127 kb on - strand, within MMJJ_RS09030 at 1667.128 kb on + strand, within MMJJ_RS09030 at 1667.128 kb on + strand, within MMJJ_RS09030 at 1667.133 kb on - strand, within MMJJ_RS09030 at 1667.151 kb on + strand, within MMJJ_RS09030 at 1667.231 kb on - strand, within MMJJ_RS09030 at 1667.232 kb on + strand, within MMJJ_RS09030 at 1667.275 kb on - strand, within MMJJ_RS09030 at 1667.309 kb on - strand, within MMJJ_RS09030 at 1667.330 kb on - strand, within MMJJ_RS09030 at 1667.330 kb on - strand, within MMJJ_RS09030 at 1667.331 kb on + strand, within MMJJ_RS09030 at 1667.357 kb on - strand, within MMJJ_RS09030 at 1667.360 kb on - strand, within MMJJ_RS09030 at 1667.360 kb on - strand, within MMJJ_RS09030 at 1667.441 kb on - strand, within MMJJ_RS09030 at 1667.474 kb on - strand, within MMJJ_RS09030 at 1667.555 kb on + strand, within MMJJ_RS09030 at 1667.649 kb on - strand, within MMJJ_RS09030 at 1667.649 kb on - strand, within MMJJ_RS09030 at 1667.684 kb on - strand, within MMJJ_RS09030 at 1667.684 kb on - strand, within MMJJ_RS09030 at 1667.684 kb on - strand, within MMJJ_RS09030 at 1667.685 kb on + strand, within MMJJ_RS09030 at 1667.685 kb on + strand, within MMJJ_RS09030 at 1667.685 kb on + strand, within MMJJ_RS09030 at 1667.685 kb on + strand, within MMJJ_RS09030 at 1667.688 kb on - strand, within MMJJ_RS09030 at 1667.691 kb on + strand, within MMJJ_RS09030 at 1667.691 kb on + strand, within MMJJ_RS09030 at 1667.702 kb on - strand, within MMJJ_RS09030 at 1667.702 kb on - strand, within MMJJ_RS09030 at 1667.709 kb on + strand, within MMJJ_RS09030 at 1667.789 kb on + strand, within MMJJ_RS09030 at 1667.818 kb on - strand, within MMJJ_RS09030 at 1667.819 kb on + strand, within MMJJ_RS09030 at 1667.864 kb on - strand, within MMJJ_RS09030 at 1667.865 kb on + strand, within MMJJ_RS09030 at 1667.886 kb on - strand, within MMJJ_RS09030 at 1667.905 kb on - strand, within MMJJ_RS09030 at 1667.924 kb on + strand, within MMJJ_RS09030 at 1667.926 kb on - strand, within MMJJ_RS09030 at 1667.928 kb on - strand, within MMJJ_RS09030 at 1667.929 kb on + strand, within MMJJ_RS09030 at 1667.929 kb on + strand, within MMJJ_RS09030 at 1667.948 kb on - strand, within MMJJ_RS09030 at 1667.960 kb on + strand, within MMJJ_RS09030 at 1667.964 kb on + strand, within MMJJ_RS09030 at 1667.987 kb on - strand at 1668.004 kb on + strand at 1668.045 kb on + strand at 1668.068 kb on + strand at 1668.127 kb on - strand at 1668.131 kb on - strand at 1668.137 kb on + strand at 1668.140 kb on + strand at 1668.140 kb on + strand at 1668.144 kb on + strand at 1668.171 kb on - strand at 1668.195 kb on + strand at 1668.251 kb on + strand at 1668.251 kb on + strand at 1668.271 kb on - strand at 1668.272 kb on + strand at 1668.272 kb on + strand at 1668.285 kb on + strand at 1668.288 kb on - strand at 1668.288 kb on - strand at 1668.288 kb on - strand at 1668.288 kb on - strand at 1668.289 kb on + strand at 1668.289 kb on + strand at 1668.290 kb on - strand at 1668.290 kb on - strand at 1668.290 kb on - strand at 1668.290 kb on - strand at 1668.290 kb on - strand at 1668.291 kb on + strand at 1668.292 kb on - strand at 1668.292 kb on - strand at 1668.292 kb on - strand at 1668.293 kb on + strand at 1668.293 kb on + strand at 1668.293 kb on + strand at 1668.301 kb on - strand at 1668.302 kb on + strand at 1668.313 kb on + strand at 1668.313 kb on + strand at 1668.314 kb on - strand at 1668.314 kb on - strand at 1668.314 kb on - strand at 1668.314 kb on - strand at 1668.314 kb on - strand at 1668.315 kb on + strand at 1668.315 kb on + strand at 1668.315 kb on + strand at 1668.315 kb on + strand at 1668.315 kb on + strand at 1668.317 kb on - strand at 1668.317 kb on - strand at 1668.317 kb on - strand at 1668.317 kb on - strand at 1668.317 kb on - strand at 1668.317 kb on - strand at 1668.317 kb on - strand at 1668.317 kb on - strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.318 kb on + strand at 1668.319 kb on - strand at 1668.319 kb on - strand at 1668.319 kb on - strand at 1668.319 kb on - strand at 1668.320 kb on + strand at 1668.321 kb on - strand at 1668.321 kb on - strand at 1668.321 kb on - strand at 1668.322 kb on + strand at 1668.353 kb on - strand at 1668.357 kb on + strand at 1668.358 kb on - strand at 1668.369 kb on - strand at 1668.370 kb on + strand at 1668.382 kb on - strand at 1669.084 kb on - strand at 1669.210 kb on + strand, within MMJJ_RS09035
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete sodium nitrite 0.125 mM remove 1,664,334 - MMJJ_RS09020 0.27 +0.4 1,664,338 + MMJJ_RS09020 0.28 +0.3 1,664,458 - MMJJ_RS09020 0.57 +2.2 1,664,459 + MMJJ_RS09020 0.57 +3.2 1,664,459 + MMJJ_RS09020 0.57 +2.5 1,664,459 + MMJJ_RS09020 0.57 -0.6 1,664,470 + MMJJ_RS09020 0.60 +0.5 1,664,482 + MMJJ_RS09020 0.63 -0.2 1,664,530 - MMJJ_RS09020 0.74 -0.1 1,664,564 - MMJJ_RS09020 0.82 -1.0 1,664,610 - +0.7 1,664,612 - +0.6 1,664,613 + -0.4 1,664,619 - -1.1 1,664,620 + +0.7 1,664,621 - -1.5 1,664,622 + -0.9 1,664,622 + -1.3 1,664,623 - +0.1 1,664,623 - -0.8 1,664,628 - -1.0 1,664,628 - +0.7 1,664,628 - -0.7 1,664,629 + -1.5 1,664,629 + +0.8 1,664,629 + +0.3 1,664,664 + +0.4 1,664,664 + -2.9 1,664,664 + -0.4 1,664,664 + -0.4 1,664,665 - +0.8 1,664,665 - -0.4 1,664,666 + -2.8 1,664,667 - -0.9 1,664,667 - -0.4 1,664,668 + -1.1 1,664,668 + +2.1 1,664,668 + +1.9 1,664,668 + -0.1 1,664,675 - +1.7 1,664,675 - -0.6 1,664,675 - -0.5 1,664,676 + -1.5 1,664,679 - -0.7 1,664,680 + -1.1 1,664,691 - -0.4 1,664,700 - -2.1 1,664,701 + -1.3 1,664,702 - -0.2 1,664,703 + +1.6 1,664,703 + +0.7 1,664,704 - +1.1 1,664,704 - -1.5 1,664,705 + -1.3 1,664,709 - -0.1 1,664,714 - -1.2 1,664,717 + -0.8 1,664,719 - -0.6 1,665,157 - +0.4 1,665,327 + -0.6 1,665,355 + -1.2 1,665,389 - -3.5 1,665,390 + -0.6 1,665,433 - +1.7 1,665,481 - -1.2 1,665,481 - -0.9 1,665,481 - -3.1 1,665,481 - -0.0 1,665,481 - -2.0 1,665,481 - -1.1 1,665,481 - -1.6 1,665,481 - -0.8 1,665,482 + +0.4 1,665,494 - -1.0 1,665,497 - -1.6 1,665,501 + +1.8 1,665,521 - +1.4 1,665,587 - +0.4 1,665,623 - MMJJ_RS09030 0.10 -1.2 1,665,644 - MMJJ_RS09030 0.11 -3.2 1,665,644 - MMJJ_RS09030 0.11 -1.2 1,665,698 - MMJJ_RS09030 0.13 -2.6 1,665,929 + MMJJ_RS09030 0.20 -2.6 1,665,989 - MMJJ_RS09030 0.23 -0.2 1,666,003 - +0.4 1,666,027 - MMJJ_RS09030 0.24 -1.3 1,666,107 - MMJJ_RS09030 0.27 -2.2 1,666,112 - MMJJ_RS09030 0.27 -1.2 1,666,227 + MMJJ_RS09030 0.31 -0.6 1,666,229 - MMJJ_RS09030 0.31 -0.4 1,666,298 - MMJJ_RS09030 0.33 -2.7 1,666,309 - MMJJ_RS09030 0.33 -2.6 1,666,309 - MMJJ_RS09030 0.33 -1.6 1,666,380 - MMJJ_RS09030 0.36 -1.2 1,666,434 - MMJJ_RS09030 0.38 -0.6 1,666,435 + MMJJ_RS09030 0.38 -2.9 1,666,461 - MMJJ_RS09030 0.39 -1.6 1,666,527 + MMJJ_RS09030 0.41 -1.6 1,666,536 - MMJJ_RS09030 0.41 +0.4 1,666,536 - MMJJ_RS09030 0.41 +1.0 1,666,536 - MMJJ_RS09030 0.41 -1.6 1,666,541 - MMJJ_RS09030 0.41 -1.2 1,666,541 - MMJJ_RS09030 0.41 -0.0 1,666,542 + MMJJ_RS09030 0.41 +0.4 1,666,542 + MMJJ_RS09030 0.41 -1.9 1,666,543 - MMJJ_RS09030 0.41 -2.2 1,666,561 + MMJJ_RS09030 0.42 -2.2 1,666,574 - MMJJ_RS09030 0.42 -0.2 1,666,578 - MMJJ_RS09030 0.43 -2.1 1,666,616 - MMJJ_RS09030 0.44 +0.4 1,666,617 + MMJJ_RS09030 0.44 -2.9 1,666,694 + MMJJ_RS09030 0.47 -1.2 1,666,695 - MMJJ_RS09030 0.47 -1.2 1,666,695 - MMJJ_RS09030 0.47 -2.2 1,666,781 - MMJJ_RS09030 0.50 -1.8 1,666,980 - MMJJ_RS09030 0.56 -1.3 1,666,981 + MMJJ_RS09030 0.56 -0.6 1,666,981 + MMJJ_RS09030 0.56 -1.6 1,667,125 - MMJJ_RS09030 0.61 -1.8 1,667,127 - MMJJ_RS09030 0.61 -2.2 1,667,127 - MMJJ_RS09030 0.61 -2.6 1,667,128 + MMJJ_RS09030 0.61 -1.6 1,667,128 + MMJJ_RS09030 0.61 -0.6 1,667,133 - MMJJ_RS09030 0.62 -0.6 1,667,151 + MMJJ_RS09030 0.62 -1.2 1,667,231 - MMJJ_RS09030 0.65 -0.6 1,667,232 + MMJJ_RS09030 0.65 -1.6 1,667,275 - MMJJ_RS09030 0.66 -1.2 1,667,309 - MMJJ_RS09030 0.68 -1.9 1,667,330 - MMJJ_RS09030 0.68 -2.7 1,667,330 - MMJJ_RS09030 0.68 -1.9 1,667,331 + MMJJ_RS09030 0.68 -2.2 1,667,357 - MMJJ_RS09030 0.69 -1.9 1,667,360 - MMJJ_RS09030 0.69 -1.9 1,667,360 - MMJJ_RS09030 0.69 -1.2 1,667,441 - MMJJ_RS09030 0.72 -0.8 1,667,474 - MMJJ_RS09030 0.73 -0.6 1,667,555 + MMJJ_RS09030 0.76 -0.8 1,667,649 - MMJJ_RS09030 0.79 +1.4 1,667,649 - MMJJ_RS09030 0.79 -0.6 1,667,684 - MMJJ_RS09030 0.80 -0.6 1,667,684 - MMJJ_RS09030 0.80 -1.6 1,667,684 - MMJJ_RS09030 0.80 -1.6 1,667,685 + MMJJ_RS09030 0.80 +1.4 1,667,685 + MMJJ_RS09030 0.80 -2.4 1,667,685 + MMJJ_RS09030 0.80 -1.4 1,667,685 + MMJJ_RS09030 0.80 -1.2 1,667,688 - MMJJ_RS09030 0.80 -2.6 1,667,691 + MMJJ_RS09030 0.81 -1.2 1,667,691 + MMJJ_RS09030 0.81 -0.6 1,667,702 - MMJJ_RS09030 0.81 -0.6 1,667,702 - MMJJ_RS09030 0.81 -1.6 1,667,709 + MMJJ_RS09030 0.81 -1.9 1,667,789 + MMJJ_RS09030 0.84 -2.8 1,667,818 - MMJJ_RS09030 0.85 -1.6 1,667,819 + MMJJ_RS09030 0.85 -2.4 1,667,864 - MMJJ_RS09030 0.86 -1.9 1,667,865 + MMJJ_RS09030 0.87 -1.9 1,667,886 - MMJJ_RS09030 0.87 -2.6 1,667,905 - MMJJ_RS09030 0.88 -1.9 1,667,924 + MMJJ_RS09030 0.89 -1.9 1,667,926 - MMJJ_RS09030 0.89 +0.4 1,667,928 - MMJJ_RS09030 0.89 -0.6 1,667,929 + MMJJ_RS09030 0.89 -2.2 1,667,929 + MMJJ_RS09030 0.89 -0.9 1,667,948 - MMJJ_RS09030 0.89 +0.4 1,667,960 + MMJJ_RS09030 0.90 -0.6 1,667,964 + MMJJ_RS09030 0.90 -1.2 1,667,987 - +0.4 1,668,004 + +0.4 1,668,045 + -1.9 1,668,068 + -0.6 1,668,127 - -2.4 1,668,131 - -1.9 1,668,137 + -1.2 1,668,140 + -1.6 1,668,140 + -1.6 1,668,144 + -1.6 1,668,171 - -0.6 1,668,195 + -1.2 1,668,251 + -1.2 1,668,251 + -1.6 1,668,271 - -1.9 1,668,272 + +2.7 1,668,272 + -3.6 1,668,285 + -2.3 1,668,288 - -0.0 1,668,288 - -1.2 1,668,288 - -1.2 1,668,288 - -1.5 1,668,289 + -1.8 1,668,289 + -0.6 1,668,290 - -2.4 1,668,290 - -2.4 1,668,290 - -4.3 1,668,290 - -0.9 1,668,290 - -1.0 1,668,291 + +0.1 1,668,292 - +0.4 1,668,292 - -0.2 1,668,292 - +0.6 1,668,293 + +0.4 1,668,293 + -2.8 1,668,293 + -1.9 1,668,301 - -2.8 1,668,302 + +0.0 1,668,313 + -0.9 1,668,313 + -2.5 1,668,314 - +1.2 1,668,314 - +0.7 1,668,314 - -1.2 1,668,314 - -0.1 1,668,314 - -2.6 1,668,315 + -0.6 1,668,315 + +0.0 1,668,315 + -1.3 1,668,315 + -1.5 1,668,315 + +1.2 1,668,317 - +0.5 1,668,317 - -0.1 1,668,317 - -1.9 1,668,317 - +2.3 1,668,317 - +2.6 1,668,317 - -0.7 1,668,317 - -2.1 1,668,317 - -2.1 1,668,318 + +3.0 1,668,318 + +1.7 1,668,318 + +0.1 1,668,318 + +0.8 1,668,318 + +0.2 1,668,318 + +3.1 1,668,318 + -2.4 1,668,318 + -0.1 1,668,318 + -0.4 1,668,318 + +2.0 1,668,318 + -0.8 1,668,318 + +0.6 1,668,318 + +1.1 1,668,319 - +0.1 1,668,319 - -0.2 1,668,319 - +0.2 1,668,319 - +0.6 1,668,320 + +0.5 1,668,321 - +1.5 1,668,321 - +0.6 1,668,321 - +2.5 1,668,322 + +1.6 1,668,353 - -0.2 1,668,357 + -1.9 1,668,358 - -0.9 1,668,369 - -0.6 1,668,370 + +7.0 1,668,382 - +0.4 1,669,084 - -0.2 1,669,210 + MMJJ_RS09035 0.41 -2.2
Or see this region's nucleotide sequence