Experiment: McCas_formate_complete sodium nitrite 0.125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04660 and MMJJ_RS04665 are separated by 15 nucleotides MMJJ_RS04665 and MMJJ_RS04670 are separated by 27 nucleotides MMJJ_RS04670 and MMJJ_RS04675 are separated by 18 nucleotides
MMJJ_RS04660: MMJJ_RS04660 - hypoxanthine/guanine phosphoribosyltransferase, at 870,231 to 870,788
_RS04660
MMJJ_RS04665: MMJJ_RS04665 - fumarylacetoacetate hydrolase family protein, at 870,804 to 871,412
_RS04665
MMJJ_RS04670: MMJJ_RS04670 - DUF2117 domain-containing protein, at 871,440 to 872,555
_RS04670
MMJJ_RS04675: MMJJ_RS04675 - thiamine pyrophosphate-binding protein, at 872,574 to 874,079
_RS04675
Position (kb) 871
872
873 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 870.465 kb on - strand, within MMJJ_RS04660 at 870.466 kb on + strand, within MMJJ_RS04660 at 870.470 kb on - strand, within MMJJ_RS04660 at 870.470 kb on - strand, within MMJJ_RS04660 at 870.470 kb on - strand, within MMJJ_RS04660 at 870.471 kb on + strand, within MMJJ_RS04660 at 870.471 kb on + strand, within MMJJ_RS04660 at 870.471 kb on + strand, within MMJJ_RS04660 at 870.471 kb on + strand, within MMJJ_RS04660 at 870.489 kb on - strand, within MMJJ_RS04660 at 870.490 kb on + strand, within MMJJ_RS04660 at 870.490 kb on + strand, within MMJJ_RS04660 at 870.549 kb on - strand, within MMJJ_RS04660 at 870.549 kb on - strand, within MMJJ_RS04660 at 870.550 kb on + strand, within MMJJ_RS04660 at 870.550 kb on + strand, within MMJJ_RS04660 at 870.553 kb on - strand, within MMJJ_RS04660 at 870.566 kb on - strand, within MMJJ_RS04660 at 870.567 kb on + strand, within MMJJ_RS04660 at 870.599 kb on - strand, within MMJJ_RS04660 at 870.602 kb on + strand, within MMJJ_RS04660 at 870.631 kb on - strand, within MMJJ_RS04660 at 870.660 kb on + strand, within MMJJ_RS04660 at 870.678 kb on + strand, within MMJJ_RS04660 at 870.678 kb on + strand, within MMJJ_RS04660 at 870.733 kb on - strand at 870.741 kb on + strand at 870.741 kb on + strand at 870.746 kb on + strand at 870.746 kb on + strand at 870.746 kb on + strand at 870.781 kb on - strand at 870.781 kb on - strand at 870.782 kb on + strand at 870.830 kb on + strand at 870.847 kb on + strand at 870.911 kb on - strand, within MMJJ_RS04665 at 870.971 kb on + strand, within MMJJ_RS04665 at 871.048 kb on - strand, within MMJJ_RS04665 at 871.048 kb on - strand, within MMJJ_RS04665 at 871.049 kb on + strand, within MMJJ_RS04665 at 871.049 kb on + strand, within MMJJ_RS04665 at 871.058 kb on - strand, within MMJJ_RS04665 at 871.066 kb on - strand, within MMJJ_RS04665 at 871.134 kb on + strand, within MMJJ_RS04665 at 871.159 kb on - strand, within MMJJ_RS04665 at 871.182 kb on + strand, within MMJJ_RS04665 at 871.246 kb on - strand, within MMJJ_RS04665 at 871.247 kb on + strand, within MMJJ_RS04665 at 871.247 kb on + strand, within MMJJ_RS04665 at 871.247 kb on + strand, within MMJJ_RS04665 at 871.310 kb on - strand, within MMJJ_RS04665 at 871.310 kb on - strand, within MMJJ_RS04665 at 871.311 kb on + strand, within MMJJ_RS04665 at 871.358 kb on - strand at 871.579 kb on - strand, within MMJJ_RS04670 at 871.580 kb on + strand, within MMJJ_RS04670 at 871.811 kb on - strand, within MMJJ_RS04670 at 871.923 kb on + strand, within MMJJ_RS04670 at 871.923 kb on + strand, within MMJJ_RS04670 at 871.923 kb on + strand, within MMJJ_RS04670 at 871.982 kb on - strand, within MMJJ_RS04670 at 872.037 kb on + strand, within MMJJ_RS04670 at 872.254 kb on - strand, within MMJJ_RS04670 at 872.255 kb on + strand, within MMJJ_RS04670 at 872.379 kb on + strand, within MMJJ_RS04670 at 872.379 kb on + strand, within MMJJ_RS04670 at 872.440 kb on - strand, within MMJJ_RS04670 at 872.526 kb on - strand at 872.545 kb on - strand at 872.559 kb on - strand at 872.591 kb on - strand at 872.883 kb on - strand, within MMJJ_RS04675 at 872.884 kb on + strand, within MMJJ_RS04675 at 872.884 kb on + strand, within MMJJ_RS04675 at 872.885 kb on - strand, within MMJJ_RS04675 at 872.898 kb on - strand, within MMJJ_RS04675 at 872.944 kb on + strand, within MMJJ_RS04675 at 872.985 kb on - strand, within MMJJ_RS04675 at 872.985 kb on - strand at 872.988 kb on + strand, within MMJJ_RS04675 at 873.047 kb on - strand, within MMJJ_RS04675 at 873.248 kb on - strand, within MMJJ_RS04675 at 873.322 kb on - strand, within MMJJ_RS04675 at 873.384 kb on - strand, within MMJJ_RS04675 at 873.390 kb on + strand, within MMJJ_RS04675 at 873.414 kb on + strand, within MMJJ_RS04675 at 873.422 kb on + strand, within MMJJ_RS04675 at 873.426 kb on + strand, within MMJJ_RS04675 at 873.430 kb on - strand, within MMJJ_RS04675 at 873.440 kb on - strand, within MMJJ_RS04675 at 873.456 kb on + strand, within MMJJ_RS04675 at 873.471 kb on + strand, within MMJJ_RS04675 at 873.481 kb on - strand, within MMJJ_RS04675 at 873.482 kb on + strand, within MMJJ_RS04675
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete sodium nitrite 0.125 mM remove 870,465 - MMJJ_RS04660 0.42 +1.0 870,466 + MMJJ_RS04660 0.42 +1.0 870,470 - MMJJ_RS04660 0.43 -0.9 870,470 - MMJJ_RS04660 0.43 -0.7 870,470 - MMJJ_RS04660 0.43 +3.0 870,471 + MMJJ_RS04660 0.43 +0.2 870,471 + MMJJ_RS04660 0.43 +2.0 870,471 + MMJJ_RS04660 0.43 -0.5 870,471 + MMJJ_RS04660 0.43 +1.6 870,489 - MMJJ_RS04660 0.46 -3.6 870,490 + MMJJ_RS04660 0.46 -0.1 870,490 + MMJJ_RS04660 0.46 +1.0 870,549 - MMJJ_RS04660 0.57 -1.0 870,549 - MMJJ_RS04660 0.57 -0.3 870,550 + MMJJ_RS04660 0.57 -0.4 870,550 + MMJJ_RS04660 0.57 +0.5 870,553 - MMJJ_RS04660 0.58 -0.5 870,566 - MMJJ_RS04660 0.60 -1.2 870,567 + MMJJ_RS04660 0.60 +0.9 870,599 - MMJJ_RS04660 0.66 +0.5 870,602 + MMJJ_RS04660 0.66 +1.3 870,631 - MMJJ_RS04660 0.72 -1.5 870,660 + MMJJ_RS04660 0.77 -0.9 870,678 + MMJJ_RS04660 0.80 -0.4 870,678 + MMJJ_RS04660 0.80 +1.2 870,733 - +0.8 870,741 + +0.5 870,741 + -0.4 870,746 + +1.8 870,746 + -1.2 870,746 + -1.1 870,781 - -0.6 870,781 - -0.5 870,782 + +1.3 870,830 + +0.1 870,847 + +1.2 870,911 - MMJJ_RS04665 0.18 -0.9 870,971 + MMJJ_RS04665 0.27 -0.4 871,048 - MMJJ_RS04665 0.40 -0.8 871,048 - MMJJ_RS04665 0.40 -0.8 871,049 + MMJJ_RS04665 0.40 -0.9 871,049 + MMJJ_RS04665 0.40 +0.9 871,058 - MMJJ_RS04665 0.42 +0.4 871,066 - MMJJ_RS04665 0.43 -2.3 871,134 + MMJJ_RS04665 0.54 +0.3 871,159 - MMJJ_RS04665 0.58 -1.0 871,182 + MMJJ_RS04665 0.62 +0.2 871,246 - MMJJ_RS04665 0.73 -1.7 871,247 + MMJJ_RS04665 0.73 +0.3 871,247 + MMJJ_RS04665 0.73 +0.9 871,247 + MMJJ_RS04665 0.73 +0.7 871,310 - MMJJ_RS04665 0.83 +0.8 871,310 - MMJJ_RS04665 0.83 -0.7 871,311 + MMJJ_RS04665 0.83 +0.8 871,358 - -1.6 871,579 - MMJJ_RS04670 0.12 -1.1 871,580 + MMJJ_RS04670 0.13 -1.5 871,811 - MMJJ_RS04670 0.33 -0.2 871,923 + MMJJ_RS04670 0.43 -2.0 871,923 + MMJJ_RS04670 0.43 -1.1 871,923 + MMJJ_RS04670 0.43 -1.5 871,982 - MMJJ_RS04670 0.49 -0.9 872,037 + MMJJ_RS04670 0.53 -0.5 872,254 - MMJJ_RS04670 0.73 -0.5 872,255 + MMJJ_RS04670 0.73 -1.5 872,379 + MMJJ_RS04670 0.84 -0.5 872,379 + MMJJ_RS04670 0.84 -0.5 872,440 - MMJJ_RS04670 0.90 -0.8 872,526 - -1.1 872,545 - -1.9 872,559 - -0.5 872,591 - +0.5 872,883 - MMJJ_RS04675 0.21 -0.5 872,884 + MMJJ_RS04675 0.21 -1.1 872,884 + MMJJ_RS04675 0.21 +2.5 872,885 - MMJJ_RS04675 0.21 -1.5 872,898 - MMJJ_RS04675 0.22 -1.5 872,944 + MMJJ_RS04675 0.25 -0.5 872,985 - MMJJ_RS04675 0.27 -3.5 872,985 - +0.5 872,988 + MMJJ_RS04675 0.27 +0.5 873,047 - MMJJ_RS04675 0.31 -1.5 873,248 - MMJJ_RS04675 0.45 -2.3 873,322 - MMJJ_RS04675 0.50 +0.5 873,384 - MMJJ_RS04675 0.54 -1.5 873,390 + MMJJ_RS04675 0.54 -1.5 873,414 + MMJJ_RS04675 0.56 -2.1 873,422 + MMJJ_RS04675 0.56 -1.1 873,426 + MMJJ_RS04675 0.57 -1.1 873,430 - MMJJ_RS04675 0.57 -0.8 873,440 - MMJJ_RS04675 0.58 -0.1 873,456 + MMJJ_RS04675 0.59 -1.1 873,471 + MMJJ_RS04675 0.60 -0.1 873,481 - MMJJ_RS04675 0.60 -0.5 873,482 + MMJJ_RS04675 0.60 -1.5
Or see this region's nucleotide sequence