Experiment: McCas_formate_complete Phenazine methosulfate 0.0166666666666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03685 and MMJJ_RS03690 overlap by 4 nucleotides MMJJ_RS03690 and MMJJ_RS03695 overlap by 8 nucleotides MMJJ_RS03695 and MMJJ_RS03700 are separated by 289 nucleotides MMJJ_RS03700 and MMJJ_RS03705 are separated by 8 nucleotides
MMJJ_RS03685: MMJJ_RS03685 - indolepyruvate ferredoxin oxidoreductase subunit alpha, at 689,060 to 690,898
_RS03685
MMJJ_RS03690: MMJJ_RS03690 - indolepyruvate oxidoreductase subunit beta, at 690,895 to 691,494
_RS03690
MMJJ_RS03695: MMJJ_RS03695 - phenylacetate--CoA ligase, at 691,487 to 692,731
_RS03695
MMJJ_RS03700: MMJJ_RS03700 - hypothetical protein, at 693,021 to 693,476
_RS03700
MMJJ_RS03705: MMJJ_RS03705 - methanogenesis marker 5 protein, at 693,485 to 693,925
_RS03705
Position (kb) 691
692
693 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 690.544 kb on - strand, within MMJJ_RS03685 at 690.545 kb on + strand, within MMJJ_RS03685 at 690.545 kb on + strand, within MMJJ_RS03685 at 690.631 kb on - strand, within MMJJ_RS03685 at 690.631 kb on - strand, within MMJJ_RS03685 at 690.631 kb on - strand, within MMJJ_RS03685 at 690.653 kb on + strand, within MMJJ_RS03685 at 690.653 kb on + strand, within MMJJ_RS03685 at 690.659 kb on - strand, within MMJJ_RS03685 at 690.661 kb on - strand, within MMJJ_RS03685 at 690.749 kb on + strand at 690.749 kb on + strand at 690.751 kb on + strand at 690.796 kb on - strand at 690.796 kb on - strand at 690.796 kb on - strand at 690.796 kb on - strand at 690.797 kb on + strand at 690.797 kb on + strand at 690.797 kb on + strand at 690.797 kb on + strand at 690.797 kb on + strand at 690.797 kb on + strand at 690.876 kb on - strand at 690.908 kb on - strand at 690.912 kb on + strand at 690.912 kb on + strand at 690.996 kb on - strand, within MMJJ_RS03690 at 691.040 kb on - strand, within MMJJ_RS03690 at 691.107 kb on + strand, within MMJJ_RS03690 at 691.110 kb on + strand, within MMJJ_RS03690 at 691.222 kb on - strand, within MMJJ_RS03690 at 691.223 kb on + strand, within MMJJ_RS03690 at 691.254 kb on - strand, within MMJJ_RS03690 at 691.255 kb on + strand, within MMJJ_RS03690 at 691.258 kb on - strand, within MMJJ_RS03690 at 691.258 kb on - strand, within MMJJ_RS03690 at 691.258 kb on - strand, within MMJJ_RS03690 at 691.259 kb on + strand, within MMJJ_RS03690 at 691.259 kb on + strand, within MMJJ_RS03690 at 691.354 kb on - strand, within MMJJ_RS03690 at 691.379 kb on + strand, within MMJJ_RS03690 at 691.379 kb on + strand, within MMJJ_RS03690 at 691.432 kb on + strand, within MMJJ_RS03690 at 691.492 kb on - strand at 691.495 kb on - strand at 691.511 kb on - strand at 691.590 kb on - strand at 691.591 kb on + strand at 691.656 kb on - strand, within MMJJ_RS03695 at 691.713 kb on - strand, within MMJJ_RS03695 at 691.757 kb on - strand, within MMJJ_RS03695 at 691.758 kb on + strand, within MMJJ_RS03695 at 691.805 kb on - strand, within MMJJ_RS03695 at 691.806 kb on + strand, within MMJJ_RS03695 at 691.834 kb on - strand at 691.835 kb on + strand, within MMJJ_RS03695 at 691.839 kb on - strand, within MMJJ_RS03695 at 691.891 kb on + strand, within MMJJ_RS03695 at 691.993 kb on - strand, within MMJJ_RS03695 at 691.994 kb on + strand, within MMJJ_RS03695 at 691.994 kb on + strand, within MMJJ_RS03695 at 692.003 kb on - strand at 692.028 kb on - strand, within MMJJ_RS03695 at 692.067 kb on - strand, within MMJJ_RS03695 at 692.074 kb on + strand, within MMJJ_RS03695 at 692.074 kb on + strand, within MMJJ_RS03695 at 692.111 kb on + strand, within MMJJ_RS03695 at 692.144 kb on - strand, within MMJJ_RS03695 at 692.166 kb on - strand, within MMJJ_RS03695 at 692.167 kb on + strand, within MMJJ_RS03695 at 692.168 kb on - strand, within MMJJ_RS03695 at 692.169 kb on + strand, within MMJJ_RS03695 at 692.216 kb on - strand, within MMJJ_RS03695 at 692.232 kb on - strand, within MMJJ_RS03695 at 692.233 kb on + strand, within MMJJ_RS03695 at 692.235 kb on - strand, within MMJJ_RS03695 at 692.257 kb on - strand, within MMJJ_RS03695 at 692.298 kb on - strand, within MMJJ_RS03695 at 692.299 kb on + strand, within MMJJ_RS03695 at 692.299 kb on + strand, within MMJJ_RS03695 at 692.312 kb on + strand, within MMJJ_RS03695 at 692.333 kb on + strand, within MMJJ_RS03695 at 692.334 kb on - strand, within MMJJ_RS03695 at 692.334 kb on - strand, within MMJJ_RS03695 at 692.335 kb on + strand, within MMJJ_RS03695 at 692.340 kb on + strand, within MMJJ_RS03695 at 692.381 kb on + strand, within MMJJ_RS03695 at 692.381 kb on + strand, within MMJJ_RS03695 at 692.434 kb on - strand, within MMJJ_RS03695 at 692.446 kb on + strand, within MMJJ_RS03695 at 692.455 kb on + strand, within MMJJ_RS03695 at 692.458 kb on - strand, within MMJJ_RS03695 at 692.458 kb on - strand, within MMJJ_RS03695 at 692.458 kb on - strand, within MMJJ_RS03695 at 692.458 kb on - strand, within MMJJ_RS03695 at 692.458 kb on - strand, within MMJJ_RS03695 at 692.458 kb on - strand, within MMJJ_RS03695 at 692.459 kb on + strand, within MMJJ_RS03695 at 692.459 kb on + strand, within MMJJ_RS03695 at 692.461 kb on + strand, within MMJJ_RS03695 at 692.461 kb on + strand, within MMJJ_RS03695 at 692.461 kb on + strand, within MMJJ_RS03695 at 692.463 kb on + strand, within MMJJ_RS03695 at 692.484 kb on - strand, within MMJJ_RS03695 at 692.484 kb on - strand, within MMJJ_RS03695 at 692.487 kb on - strand, within MMJJ_RS03695 at 692.488 kb on + strand, within MMJJ_RS03695 at 692.491 kb on - strand, within MMJJ_RS03695 at 692.492 kb on + strand, within MMJJ_RS03695 at 692.492 kb on + strand, within MMJJ_RS03695 at 692.496 kb on - strand, within MMJJ_RS03695 at 692.497 kb on + strand, within MMJJ_RS03695 at 692.506 kb on + strand, within MMJJ_RS03695 at 692.506 kb on + strand, within MMJJ_RS03695 at 692.510 kb on + strand, within MMJJ_RS03695 at 692.525 kb on + strand, within MMJJ_RS03695 at 692.554 kb on - strand, within MMJJ_RS03695 at 692.555 kb on + strand, within MMJJ_RS03695 at 692.555 kb on + strand, within MMJJ_RS03695 at 692.559 kb on - strand, within MMJJ_RS03695 at 692.559 kb on - strand, within MMJJ_RS03695 at 692.560 kb on + strand, within MMJJ_RS03695 at 692.564 kb on + strand, within MMJJ_RS03695 at 692.568 kb on - strand, within MMJJ_RS03695 at 692.569 kb on + strand, within MMJJ_RS03695 at 692.619 kb on - strand at 692.619 kb on - strand at 692.619 kb on - strand at 692.620 kb on + strand at 692.620 kb on + strand at 692.636 kb on - strand at 692.636 kb on - strand at 692.637 kb on + strand at 692.641 kb on + strand at 692.704 kb on - strand at 692.730 kb on + strand at 692.774 kb on - strand at 692.777 kb on - strand at 692.777 kb on - strand at 692.778 kb on + strand at 692.849 kb on + strand at 692.852 kb on - strand at 692.855 kb on - strand at 692.855 kb on - strand at 692.856 kb on + strand at 692.885 kb on + strand at 692.904 kb on - strand at 692.905 kb on + strand at 692.906 kb on - strand at 692.906 kb on - strand at 692.906 kb on - strand at 692.906 kb on - strand at 692.906 kb on - strand at 692.906 kb on - strand at 692.907 kb on + strand at 692.907 kb on + strand at 692.907 kb on + strand at 692.908 kb on - strand at 692.909 kb on + strand at 692.909 kb on + strand at 692.909 kb on + strand at 692.909 kb on + strand at 692.915 kb on - strand at 692.915 kb on - strand at 692.916 kb on + strand at 692.916 kb on + strand at 692.919 kb on - strand at 692.920 kb on + strand at 692.923 kb on - strand at 692.924 kb on + strand at 692.955 kb on + strand at 692.955 kb on + strand at 692.956 kb on - strand at 692.957 kb on + strand at 692.959 kb on + strand at 692.960 kb on - strand at 692.961 kb on + strand at 692.961 kb on + strand at 692.996 kb on + strand at 693.016 kb on - strand at 693.016 kb on - strand at 693.018 kb on - strand at 693.018 kb on - strand at 693.018 kb on - strand at 693.018 kb on - strand at 693.036 kb on - strand at 693.036 kb on - strand at 693.039 kb on - strand at 693.040 kb on + strand at 693.040 kb on + strand at 693.045 kb on - strand at 693.045 kb on - strand at 693.046 kb on + strand at 693.046 kb on + strand at 693.046 kb on + strand at 693.079 kb on + strand, within MMJJ_RS03700 at 693.129 kb on - strand, within MMJJ_RS03700 at 693.177 kb on + strand, within MMJJ_RS03700 at 693.178 kb on - strand, within MMJJ_RS03700 at 693.178 kb on - strand, within MMJJ_RS03700 at 693.179 kb on + strand, within MMJJ_RS03700 at 693.179 kb on + strand, within MMJJ_RS03700 at 693.182 kb on - strand, within MMJJ_RS03700 at 693.182 kb on - strand, within MMJJ_RS03700 at 693.183 kb on + strand, within MMJJ_RS03700 at 693.183 kb on + strand, within MMJJ_RS03700 at 693.183 kb on + strand, within MMJJ_RS03700 at 693.227 kb on - strand, within MMJJ_RS03700 at 693.227 kb on - strand, within MMJJ_RS03700 at 693.228 kb on + strand, within MMJJ_RS03700 at 693.285 kb on - strand, within MMJJ_RS03700 at 693.409 kb on + strand, within MMJJ_RS03700 at 693.458 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Phenazine methosulfate 0.0166666666666667 mM remove 690,544 - MMJJ_RS03685 0.81 +1.4 690,545 + MMJJ_RS03685 0.81 +0.6 690,545 + MMJJ_RS03685 0.81 +0.2 690,631 - MMJJ_RS03685 0.85 -0.4 690,631 - MMJJ_RS03685 0.85 -0.8 690,631 - MMJJ_RS03685 0.85 -0.6 690,653 + MMJJ_RS03685 0.87 -1.3 690,653 + MMJJ_RS03685 0.87 -3.2 690,659 - MMJJ_RS03685 0.87 -2.5 690,661 - MMJJ_RS03685 0.87 -1.7 690,749 + +2.0 690,749 + -0.8 690,751 + -3.0 690,796 - -0.4 690,796 - -0.5 690,796 - +0.5 690,796 - +0.3 690,797 + +0.2 690,797 + -0.0 690,797 + -2.1 690,797 + +1.7 690,797 + +0.2 690,797 + -0.4 690,876 - +0.1 690,908 - +1.2 690,912 + -1.4 690,912 + -3.0 690,996 - MMJJ_RS03690 0.17 -2.1 691,040 - MMJJ_RS03690 0.24 +2.0 691,107 + MMJJ_RS03690 0.35 -3.2 691,110 + MMJJ_RS03690 0.36 -0.4 691,222 - MMJJ_RS03690 0.55 -1.4 691,223 + MMJJ_RS03690 0.55 -0.5 691,254 - MMJJ_RS03690 0.60 -0.1 691,255 + MMJJ_RS03690 0.60 +0.2 691,258 - MMJJ_RS03690 0.60 +0.3 691,258 - MMJJ_RS03690 0.60 +0.6 691,258 - MMJJ_RS03690 0.60 -1.2 691,259 + MMJJ_RS03690 0.61 -1.1 691,259 + MMJJ_RS03690 0.61 -0.4 691,354 - MMJJ_RS03690 0.77 -0.0 691,379 + MMJJ_RS03690 0.81 -1.5 691,379 + MMJJ_RS03690 0.81 -2.0 691,432 + MMJJ_RS03690 0.90 -4.0 691,492 - +1.9 691,495 - -2.5 691,511 - +0.4 691,590 - +1.5 691,591 + -1.4 691,656 - MMJJ_RS03695 0.14 -0.4 691,713 - MMJJ_RS03695 0.18 -1.6 691,757 - MMJJ_RS03695 0.22 +1.0 691,758 + MMJJ_RS03695 0.22 -2.1 691,805 - MMJJ_RS03695 0.26 +0.6 691,806 + MMJJ_RS03695 0.26 -1.7 691,834 - +1.2 691,835 + MMJJ_RS03695 0.28 -1.4 691,839 - MMJJ_RS03695 0.28 +0.4 691,891 + MMJJ_RS03695 0.32 -3.2 691,993 - MMJJ_RS03695 0.41 -0.7 691,994 + MMJJ_RS03695 0.41 +1.0 691,994 + MMJJ_RS03695 0.41 -3.9 692,003 - +0.2 692,028 - MMJJ_RS03695 0.43 +1.8 692,067 - MMJJ_RS03695 0.47 +2.6 692,074 + MMJJ_RS03695 0.47 +0.3 692,074 + MMJJ_RS03695 0.47 +0.5 692,111 + MMJJ_RS03695 0.50 -0.2 692,144 - MMJJ_RS03695 0.53 -2.9 692,166 - MMJJ_RS03695 0.55 -2.9 692,167 + MMJJ_RS03695 0.55 +1.9 692,168 - MMJJ_RS03695 0.55 -1.5 692,169 + MMJJ_RS03695 0.55 +0.4 692,216 - MMJJ_RS03695 0.59 -3.1 692,232 - MMJJ_RS03695 0.60 -3.2 692,233 + MMJJ_RS03695 0.60 -3.8 692,235 - MMJJ_RS03695 0.60 -0.4 692,257 - MMJJ_RS03695 0.62 -1.0 692,298 - MMJJ_RS03695 0.65 -3.6 692,299 + MMJJ_RS03695 0.65 -2.0 692,299 + MMJJ_RS03695 0.65 +0.1 692,312 + MMJJ_RS03695 0.66 -1.4 692,333 + MMJJ_RS03695 0.68 -3.6 692,334 - MMJJ_RS03695 0.68 -0.2 692,334 - MMJJ_RS03695 0.68 -3.5 692,335 + MMJJ_RS03695 0.68 -1.2 692,340 + MMJJ_RS03695 0.69 -2.5 692,381 + MMJJ_RS03695 0.72 -1.4 692,381 + MMJJ_RS03695 0.72 +0.6 692,434 - MMJJ_RS03695 0.76 -0.3 692,446 + MMJJ_RS03695 0.77 +1.8 692,455 + MMJJ_RS03695 0.78 +0.1 692,458 - MMJJ_RS03695 0.78 -2.0 692,458 - MMJJ_RS03695 0.78 -3.1 692,458 - MMJJ_RS03695 0.78 -2.9 692,458 - MMJJ_RS03695 0.78 +1.3 692,458 - MMJJ_RS03695 0.78 -2.4 692,458 - MMJJ_RS03695 0.78 -0.8 692,459 + MMJJ_RS03695 0.78 -0.1 692,459 + MMJJ_RS03695 0.78 -1.1 692,461 + MMJJ_RS03695 0.78 +0.9 692,461 + MMJJ_RS03695 0.78 -0.9 692,461 + MMJJ_RS03695 0.78 +1.1 692,463 + MMJJ_RS03695 0.78 -1.1 692,484 - MMJJ_RS03695 0.80 +0.1 692,484 - MMJJ_RS03695 0.80 +1.2 692,487 - MMJJ_RS03695 0.80 +1.5 692,488 + MMJJ_RS03695 0.80 -0.4 692,491 - MMJJ_RS03695 0.81 +0.9 692,492 + MMJJ_RS03695 0.81 -3.5 692,492 + MMJJ_RS03695 0.81 +0.0 692,496 - MMJJ_RS03695 0.81 -0.5 692,497 + MMJJ_RS03695 0.81 +1.5 692,506 + MMJJ_RS03695 0.82 -1.7 692,506 + MMJJ_RS03695 0.82 -3.0 692,510 + MMJJ_RS03695 0.82 -1.1 692,525 + MMJJ_RS03695 0.83 -2.4 692,554 - MMJJ_RS03695 0.86 +1.2 692,555 + MMJJ_RS03695 0.86 +1.6 692,555 + MMJJ_RS03695 0.86 -1.9 692,559 - MMJJ_RS03695 0.86 +1.4 692,559 - MMJJ_RS03695 0.86 -2.0 692,560 + MMJJ_RS03695 0.86 +0.2 692,564 + MMJJ_RS03695 0.87 -1.5 692,568 - MMJJ_RS03695 0.87 -0.8 692,569 + MMJJ_RS03695 0.87 +3.4 692,619 - -3.3 692,619 - -2.4 692,619 - +2.8 692,620 + -2.6 692,620 + +1.8 692,636 - +2.2 692,636 - -1.4 692,637 + -1.0 692,641 + -0.4 692,704 - -2.6 692,730 + +2.1 692,774 - -1.5 692,777 - -2.4 692,777 - +0.6 692,778 + +2.0 692,849 + -3.8 692,852 - -1.7 692,855 - -0.2 692,855 - +0.6 692,856 + -3.3 692,885 + +1.3 692,904 - +1.0 692,905 + +1.3 692,906 - +0.3 692,906 - -2.6 692,906 - +1.4 692,906 - -1.9 692,906 - -0.9 692,906 - -2.1 692,907 + -0.1 692,907 + +2.0 692,907 + +0.8 692,908 - -1.6 692,909 + -0.9 692,909 + -2.8 692,909 + -2.1 692,909 + +1.2 692,915 - +1.6 692,915 - +1.3 692,916 + -1.1 692,916 + -1.4 692,919 - +0.9 692,920 + +0.9 692,923 - -0.5 692,924 + +2.1 692,955 + +1.7 692,955 + +0.0 692,956 - +1.3 692,957 + +0.3 692,959 + -4.4 692,960 - -0.8 692,961 + +0.3 692,961 + +2.0 692,996 + -0.1 693,016 - -0.1 693,016 - -2.8 693,018 - +1.4 693,018 - -1.1 693,018 - -1.3 693,018 - +2.0 693,036 - +1.9 693,036 - -1.1 693,039 - +1.6 693,040 + +2.4 693,040 + -2.4 693,045 - +0.0 693,045 - -1.9 693,046 + -0.2 693,046 + +1.2 693,046 + +1.0 693,079 + MMJJ_RS03700 0.13 -0.8 693,129 - MMJJ_RS03700 0.24 -2.0 693,177 + MMJJ_RS03700 0.34 +0.5 693,178 - MMJJ_RS03700 0.34 -2.6 693,178 - MMJJ_RS03700 0.34 -3.3 693,179 + MMJJ_RS03700 0.35 -1.7 693,179 + MMJJ_RS03700 0.35 -0.5 693,182 - MMJJ_RS03700 0.35 +2.0 693,182 - MMJJ_RS03700 0.35 -1.5 693,183 + MMJJ_RS03700 0.36 -0.4 693,183 + MMJJ_RS03700 0.36 -0.8 693,183 + MMJJ_RS03700 0.36 -1.8 693,227 - MMJJ_RS03700 0.45 -0.2 693,227 - MMJJ_RS03700 0.45 +3.4 693,228 + MMJJ_RS03700 0.45 +1.1 693,285 - MMJJ_RS03700 0.58 -1.5 693,409 + MMJJ_RS03700 0.85 -1.1 693,458 - -1.5
Or see this region's nucleotide sequence