Experiment: Survival; Treatment=FW106 (pH3.9) with 1 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_01490 and IAI47_01495 are separated by 47 nucleotides IAI47_01495 and IAI47_01500 are separated by 49 nucleotides IAI47_01500 and IAI47_01505 are separated by 323 nucleotides
IAI47_01490: IAI47_01490 - Gfo/Idh/MocA family oxidoreductase, at 325,974 to 326,984
_01490
IAI47_01495: IAI47_01495 - myo-inosose-2 dehydratase, at 327,032 to 327,928
_01495
IAI47_01500: IAI47_01500 - DcrB family lipoprotein, at 327,978 to 328,532
_01500
IAI47_01505: IAI47_01505 - 7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter, at 328,856 to 329,521
_01505
Position (kb) 327
328
329 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 326.978 kb on + strand at 326.978 kb on + strand at 326.979 kb on - strand at 326.979 kb on - strand at 326.979 kb on - strand at 326.995 kb on + strand at 326.995 kb on + strand at 326.996 kb on - strand at 327.049 kb on - strand at 327.112 kb on + strand at 327.113 kb on - strand at 327.113 kb on - strand at 327.155 kb on - strand, within IAI47_01495 at 327.179 kb on + strand, within IAI47_01495 at 327.361 kb on + strand, within IAI47_01495 at 327.361 kb on + strand, within IAI47_01495 at 327.361 kb on + strand, within IAI47_01495 at 327.362 kb on - strand, within IAI47_01495 at 327.367 kb on + strand, within IAI47_01495 at 327.367 kb on + strand, within IAI47_01495 at 327.368 kb on - strand, within IAI47_01495 at 327.368 kb on - strand, within IAI47_01495 at 327.432 kb on + strand, within IAI47_01495 at 327.433 kb on - strand, within IAI47_01495 at 327.446 kb on + strand, within IAI47_01495 at 327.547 kb on + strand, within IAI47_01495 at 327.581 kb on + strand, within IAI47_01495 at 327.581 kb on + strand, within IAI47_01495 at 327.581 kb on + strand, within IAI47_01495 at 327.582 kb on - strand, within IAI47_01495 at 327.582 kb on - strand, within IAI47_01495 at 327.582 kb on - strand, within IAI47_01495 at 327.582 kb on - strand, within IAI47_01495 at 327.599 kb on + strand, within IAI47_01495 at 327.599 kb on + strand, within IAI47_01495 at 327.599 kb on + strand, within IAI47_01495 at 327.600 kb on - strand, within IAI47_01495 at 327.600 kb on - strand, within IAI47_01495 at 327.600 kb on - strand, within IAI47_01495 at 327.600 kb on - strand, within IAI47_01495 at 327.600 kb on - strand, within IAI47_01495 at 327.602 kb on + strand, within IAI47_01495 at 327.602 kb on + strand, within IAI47_01495 at 327.648 kb on - strand, within IAI47_01495 at 327.648 kb on - strand, within IAI47_01495 at 327.757 kb on + strand, within IAI47_01495 at 327.800 kb on - strand, within IAI47_01495 at 327.815 kb on + strand, within IAI47_01495 at 327.816 kb on - strand, within IAI47_01495 at 327.821 kb on + strand, within IAI47_01495 at 327.821 kb on + strand, within IAI47_01495 at 327.822 kb on - strand, within IAI47_01495 at 327.881 kb on + strand at 327.881 kb on + strand at 327.902 kb on + strand at 327.903 kb on - strand at 327.903 kb on - strand at 327.905 kb on - strand at 327.916 kb on + strand at 327.924 kb on + strand at 327.924 kb on + strand at 327.924 kb on + strand at 327.924 kb on + strand at 327.924 kb on + strand at 327.926 kb on + strand at 327.926 kb on + strand at 327.927 kb on - strand at 327.980 kb on - strand at 327.980 kb on - strand at 327.980 kb on - strand at 327.989 kb on + strand at 327.989 kb on + strand at 327.990 kb on - strand at 327.990 kb on - strand at 327.990 kb on - strand at 328.037 kb on + strand, within IAI47_01500 at 328.052 kb on + strand, within IAI47_01500 at 328.053 kb on - strand, within IAI47_01500 at 328.053 kb on - strand, within IAI47_01500 at 328.053 kb on - strand, within IAI47_01500 at 328.085 kb on + strand, within IAI47_01500 at 328.086 kb on - strand, within IAI47_01500 at 328.137 kb on - strand, within IAI47_01500 at 328.212 kb on - strand, within IAI47_01500 at 328.313 kb on - strand, within IAI47_01500 at 328.328 kb on + strand, within IAI47_01500 at 328.328 kb on + strand, within IAI47_01500 at 328.328 kb on + strand, within IAI47_01500 at 328.328 kb on + strand, within IAI47_01500 at 328.346 kb on + strand, within IAI47_01500 at 328.356 kb on - strand, within IAI47_01500 at 328.356 kb on - strand, within IAI47_01500 at 328.482 kb on + strand at 328.491 kb on + strand at 328.491 kb on + strand at 328.497 kb on + strand at 328.497 kb on + strand at 328.498 kb on - strand at 328.509 kb on + strand at 328.509 kb on + strand at 328.509 kb on + strand at 328.509 kb on + strand at 328.509 kb on + strand at 328.510 kb on - strand at 328.510 kb on - strand at 328.510 kb on - strand at 328.510 kb on - strand at 328.510 kb on - strand at 328.510 kb on - strand at 328.510 kb on - strand at 328.513 kb on + strand at 328.513 kb on + strand at 328.513 kb on + strand at 328.513 kb on + strand at 328.513 kb on + strand at 328.513 kb on + strand at 328.514 kb on - strand at 328.514 kb on - strand at 328.514 kb on - strand at 328.514 kb on - strand at 328.514 kb on - strand at 328.546 kb on + strand at 328.547 kb on - strand at 328.547 kb on - strand at 328.565 kb on + strand at 328.565 kb on + strand at 328.566 kb on - strand at 328.566 kb on - strand at 328.568 kb on + strand at 328.568 kb on + strand at 328.568 kb on + strand at 328.568 kb on + strand at 328.568 kb on + strand at 328.568 kb on + strand at 328.569 kb on - strand at 328.569 kb on - strand at 328.569 kb on - strand at 328.569 kb on - strand at 328.569 kb on - strand at 328.569 kb on - strand at 328.569 kb on - strand at 328.569 kb on - strand at 328.569 kb on - strand at 328.569 kb on - strand at 328.618 kb on + strand at 328.641 kb on - strand at 328.641 kb on - strand at 328.641 kb on - strand at 328.641 kb on - strand at 328.650 kb on + strand at 328.670 kb on + strand at 328.671 kb on - strand at 328.718 kb on - strand at 328.718 kb on - strand at 328.768 kb on + strand at 328.768 kb on + strand at 328.782 kb on + strand at 328.782 kb on + strand at 328.783 kb on - strand at 328.783 kb on - strand at 328.783 kb on - strand at 328.783 kb on - strand at 328.783 kb on - strand at 328.790 kb on - strand at 328.790 kb on - strand at 328.790 kb on - strand at 328.790 kb on - strand at 328.792 kb on + strand at 328.793 kb on - strand at 328.836 kb on + strand at 328.837 kb on - strand at 328.837 kb on - strand at 328.837 kb on - strand at 328.837 kb on - strand at 328.846 kb on + strand at 328.847 kb on - strand at 328.922 kb on + strand at 328.923 kb on - strand, within IAI47_01505 at 328.923 kb on - strand, within IAI47_01505 at 328.923 kb on - strand, within IAI47_01505 at 328.923 kb on - strand, within IAI47_01505 at 328.923 kb on - strand, within IAI47_01505 at 328.929 kb on + strand, within IAI47_01505 at 328.957 kb on + strand, within IAI47_01505 at 328.957 kb on + strand, within IAI47_01505 at 328.958 kb on - strand, within IAI47_01505 at 328.958 kb on - strand, within IAI47_01505 at 328.958 kb on - strand, within IAI47_01505 at 328.958 kb on - strand, within IAI47_01505 at 328.958 kb on - strand, within IAI47_01505 at 328.958 kb on - strand, within IAI47_01505 at 328.958 kb on - strand, within IAI47_01505 at 328.974 kb on + strand, within IAI47_01505 at 328.974 kb on + strand, within IAI47_01505 at 328.975 kb on - strand, within IAI47_01505 at 329.012 kb on - strand, within IAI47_01505 at 329.031 kb on + strand, within IAI47_01505 at 329.031 kb on + strand, within IAI47_01505 at 329.031 kb on + strand, within IAI47_01505 at 329.032 kb on - strand, within IAI47_01505 at 329.059 kb on + strand, within IAI47_01505 at 329.059 kb on + strand, within IAI47_01505 at 329.059 kb on + strand, within IAI47_01505 at 329.068 kb on + strand, within IAI47_01505 at 329.068 kb on + strand, within IAI47_01505 at 329.068 kb on + strand, within IAI47_01505 at 329.069 kb on - strand, within IAI47_01505 at 329.099 kb on + strand, within IAI47_01505 at 329.099 kb on + strand, within IAI47_01505 at 329.099 kb on + strand, within IAI47_01505 at 329.100 kb on - strand, within IAI47_01505 at 329.100 kb on - strand, within IAI47_01505 at 329.100 kb on - strand, within IAI47_01505 at 329.135 kb on + strand, within IAI47_01505 at 329.135 kb on + strand, within IAI47_01505 at 329.135 kb on + strand, within IAI47_01505 at 329.136 kb on - strand, within IAI47_01505 at 329.136 kb on - strand, within IAI47_01505 at 329.136 kb on - strand, within IAI47_01505 at 329.136 kb on - strand, within IAI47_01505 at 329.167 kb on - strand, within IAI47_01505 at 329.167 kb on - strand, within IAI47_01505 at 329.250 kb on + strand, within IAI47_01505 at 329.251 kb on - strand, within IAI47_01505 at 329.251 kb on - strand, within IAI47_01505 at 329.251 kb on - strand, within IAI47_01505 at 329.251 kb on - strand, within IAI47_01505 at 329.251 kb on - strand, within IAI47_01505 at 329.251 kb on - strand, within IAI47_01505 at 329.271 kb on + strand, within IAI47_01505 at 329.272 kb on - strand, within IAI47_01505 at 329.272 kb on - strand, within IAI47_01505 at 329.279 kb on - strand, within IAI47_01505 at 329.279 kb on - strand, within IAI47_01505 at 329.308 kb on + strand, within IAI47_01505 at 329.308 kb on + strand, within IAI47_01505 at 329.308 kb on + strand, within IAI47_01505 at 329.309 kb on - strand, within IAI47_01505 at 329.338 kb on + strand, within IAI47_01505 at 329.338 kb on + strand, within IAI47_01505 at 329.339 kb on - strand, within IAI47_01505 at 329.339 kb on - strand, within IAI47_01505 at 329.339 kb on - strand, within IAI47_01505 at 329.368 kb on + strand, within IAI47_01505 at 329.369 kb on - strand, within IAI47_01505 at 329.436 kb on + strand, within IAI47_01505 at 329.436 kb on + strand, within IAI47_01505 at 329.436 kb on + strand, within IAI47_01505 at 329.437 kb on - strand, within IAI47_01505 at 329.437 kb on - strand, within IAI47_01505 at 329.437 kb on - strand, within IAI47_01505 at 329.437 kb on - strand, within IAI47_01505 at 329.440 kb on + strand, within IAI47_01505 at 329.440 kb on + strand, within IAI47_01505 at 329.440 kb on + strand, within IAI47_01505 at 329.440 kb on + strand, within IAI47_01505 at 329.440 kb on + strand, within IAI47_01505 at 329.440 kb on + strand, within IAI47_01505 at 329.441 kb on - strand, within IAI47_01505 at 329.441 kb on - strand, within IAI47_01505 at 329.441 kb on - strand, within IAI47_01505 at 329.441 kb on - strand, within IAI47_01505 at 329.441 kb on - strand, within IAI47_01505 at 329.521 kb on + strand at 329.521 kb on + strand at 329.521 kb on + strand at 329.521 kb on + strand at 329.521 kb on + strand at 329.522 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW106 (pH3.9) with 1 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 326,978 + +1.6 326,978 + -1.0 326,979 - -1.6 326,979 - +1.1 326,979 - -1.4 326,995 + -0.3 326,995 + -0.5 326,996 - +0.8 327,049 - -1.2 327,112 + -0.4 327,113 - -0.5 327,113 - -0.1 327,155 - IAI47_01495 0.14 -1.0 327,179 + IAI47_01495 0.16 -0.6 327,361 + IAI47_01495 0.37 -0.5 327,361 + IAI47_01495 0.37 +0.8 327,361 + IAI47_01495 0.37 +1.4 327,362 - IAI47_01495 0.37 -0.6 327,367 + IAI47_01495 0.37 -1.9 327,367 + IAI47_01495 0.37 -1.6 327,368 - IAI47_01495 0.37 -2.2 327,368 - IAI47_01495 0.37 -0.5 327,432 + IAI47_01495 0.45 -1.7 327,433 - IAI47_01495 0.45 -1.0 327,446 + IAI47_01495 0.46 +1.3 327,547 + IAI47_01495 0.57 +1.0 327,581 + IAI47_01495 0.61 -0.9 327,581 + IAI47_01495 0.61 +0.5 327,581 + IAI47_01495 0.61 -1.2 327,582 - IAI47_01495 0.61 -1.8 327,582 - IAI47_01495 0.61 -0.8 327,582 - IAI47_01495 0.61 -0.9 327,582 - IAI47_01495 0.61 -0.6 327,599 + IAI47_01495 0.63 +1.3 327,599 + IAI47_01495 0.63 -0.0 327,599 + IAI47_01495 0.63 -2.3 327,600 - IAI47_01495 0.63 +1.5 327,600 - IAI47_01495 0.63 +0.3 327,600 - IAI47_01495 0.63 -1.2 327,600 - IAI47_01495 0.63 -1.1 327,600 - IAI47_01495 0.63 -1.3 327,602 + IAI47_01495 0.64 -0.2 327,602 + IAI47_01495 0.64 -1.4 327,648 - IAI47_01495 0.69 -2.4 327,648 - IAI47_01495 0.69 -0.5 327,757 + IAI47_01495 0.81 -1.8 327,800 - IAI47_01495 0.86 -0.6 327,815 + IAI47_01495 0.87 +1.1 327,816 - IAI47_01495 0.87 -1.1 327,821 + IAI47_01495 0.88 -1.4 327,821 + IAI47_01495 0.88 +1.0 327,822 - IAI47_01495 0.88 +1.1 327,881 + -0.7 327,881 + +0.5 327,902 + -0.6 327,903 - +0.0 327,903 - +1.7 327,905 - -0.7 327,916 + +0.9 327,924 + +0.3 327,924 + -0.0 327,924 + -2.5 327,924 + +1.0 327,924 + +0.8 327,926 + -0.6 327,926 + +0.2 327,927 - -1.0 327,980 - +0.2 327,980 - +0.4 327,980 - +0.2 327,989 + -1.2 327,989 + -1.0 327,990 - +0.7 327,990 - +0.6 327,990 - +1.0 328,037 + IAI47_01500 0.11 -1.6 328,052 + IAI47_01500 0.13 -1.4 328,053 - IAI47_01500 0.14 +1.2 328,053 - IAI47_01500 0.14 -1.7 328,053 - IAI47_01500 0.14 +0.1 328,085 + IAI47_01500 0.19 -1.3 328,086 - IAI47_01500 0.19 +1.9 328,137 - IAI47_01500 0.29 -0.1 328,212 - IAI47_01500 0.42 -0.2 328,313 - IAI47_01500 0.60 -0.9 328,328 + IAI47_01500 0.63 -0.8 328,328 + IAI47_01500 0.63 -2.3 328,328 + IAI47_01500 0.63 -0.4 328,328 + IAI47_01500 0.63 -0.7 328,346 + IAI47_01500 0.66 +2.1 328,356 - IAI47_01500 0.68 +0.9 328,356 - IAI47_01500 0.68 +4.8 328,482 + -1.7 328,491 + +1.0 328,491 + +1.0 328,497 + -1.3 328,497 + -1.6 328,498 - -0.4 328,509 + -0.9 328,509 + +1.4 328,509 + -0.8 328,509 + -0.5 328,509 + -0.5 328,510 - -0.2 328,510 - -0.5 328,510 - +1.6 328,510 - +1.9 328,510 - +1.8 328,510 - +1.3 328,510 - -0.0 328,513 + -0.6 328,513 + +1.3 328,513 + -1.5 328,513 + -1.7 328,513 + -0.2 328,513 + -0.5 328,514 - -2.2 328,514 - -0.4 328,514 - +0.1 328,514 - +1.1 328,514 - -1.2 328,546 + +1.3 328,547 - +0.4 328,547 - +0.2 328,565 + +0.2 328,565 + -0.4 328,566 - -0.5 328,566 - -0.8 328,568 + -2.7 328,568 + -1.4 328,568 + -1.3 328,568 + -0.6 328,568 + -0.4 328,568 + -2.5 328,569 - +1.1 328,569 - -0.9 328,569 - -1.7 328,569 - +0.6 328,569 - -1.8 328,569 - +0.2 328,569 - -1.6 328,569 - -0.1 328,569 - +2.5 328,569 - -1.5 328,618 + -0.2 328,641 - -1.4 328,641 - -0.2 328,641 - +2.8 328,641 - -0.7 328,650 + -1.4 328,670 + +0.2 328,671 - -0.8 328,718 - -0.3 328,718 - +0.1 328,768 + +2.1 328,768 + +0.1 328,782 + +3.8 328,782 + -3.0 328,783 - -2.4 328,783 - -0.1 328,783 - -0.8 328,783 - -0.5 328,783 - -0.2 328,790 - +0.9 328,790 - -1.4 328,790 - +0.0 328,790 - +0.4 328,792 + -0.2 328,793 - -1.2 328,836 + -0.7 328,837 - -2.6 328,837 - -0.2 328,837 - +0.5 328,837 - -1.3 328,846 + +1.2 328,847 - -0.2 328,922 + +1.2 328,923 - IAI47_01505 0.10 -0.4 328,923 - IAI47_01505 0.10 -1.6 328,923 - IAI47_01505 0.10 +0.9 328,923 - IAI47_01505 0.10 +0.5 328,923 - IAI47_01505 0.10 +0.7 328,929 + IAI47_01505 0.11 -0.6 328,957 + IAI47_01505 0.15 -0.7 328,957 + IAI47_01505 0.15 +1.0 328,958 - IAI47_01505 0.15 -1.7 328,958 - IAI47_01505 0.15 -0.2 328,958 - IAI47_01505 0.15 -0.9 328,958 - IAI47_01505 0.15 -1.3 328,958 - IAI47_01505 0.15 -2.9 328,958 - IAI47_01505 0.15 +1.3 328,958 - IAI47_01505 0.15 -0.8 328,974 + IAI47_01505 0.18 +1.8 328,974 + IAI47_01505 0.18 +1.4 328,975 - IAI47_01505 0.18 +0.6 329,012 - IAI47_01505 0.23 -0.2 329,031 + IAI47_01505 0.26 +0.4 329,031 + IAI47_01505 0.26 -1.5 329,031 + IAI47_01505 0.26 +0.5 329,032 - IAI47_01505 0.26 +1.8 329,059 + IAI47_01505 0.30 -0.7 329,059 + IAI47_01505 0.30 -0.9 329,059 + IAI47_01505 0.30 +0.3 329,068 + IAI47_01505 0.32 -1.9 329,068 + IAI47_01505 0.32 -1.4 329,068 + IAI47_01505 0.32 -1.7 329,069 - IAI47_01505 0.32 -1.7 329,099 + IAI47_01505 0.36 -1.8 329,099 + IAI47_01505 0.36 +0.5 329,099 + IAI47_01505 0.36 -1.1 329,100 - IAI47_01505 0.37 -0.7 329,100 - IAI47_01505 0.37 -0.1 329,100 - IAI47_01505 0.37 -0.5 329,135 + IAI47_01505 0.42 +1.9 329,135 + IAI47_01505 0.42 -0.5 329,135 + IAI47_01505 0.42 +0.2 329,136 - IAI47_01505 0.42 -1.1 329,136 - IAI47_01505 0.42 +0.1 329,136 - IAI47_01505 0.42 +0.5 329,136 - IAI47_01505 0.42 +2.0 329,167 - IAI47_01505 0.47 -1.8 329,167 - IAI47_01505 0.47 +0.1 329,250 + IAI47_01505 0.59 +0.4 329,251 - IAI47_01505 0.59 -1.2 329,251 - IAI47_01505 0.59 -0.6 329,251 - IAI47_01505 0.59 +1.6 329,251 - IAI47_01505 0.59 -1.4 329,251 - IAI47_01505 0.59 -0.3 329,251 - IAI47_01505 0.59 -1.2 329,271 + IAI47_01505 0.62 -2.5 329,272 - IAI47_01505 0.62 -0.9 329,272 - IAI47_01505 0.62 -1.4 329,279 - IAI47_01505 0.64 +0.2 329,279 - IAI47_01505 0.64 +0.7 329,308 + IAI47_01505 0.68 -0.5 329,308 + IAI47_01505 0.68 -1.1 329,308 + IAI47_01505 0.68 -0.5 329,309 - IAI47_01505 0.68 -0.7 329,338 + IAI47_01505 0.72 +0.4 329,338 + IAI47_01505 0.72 +0.3 329,339 - IAI47_01505 0.73 +2.1 329,339 - IAI47_01505 0.73 +2.2 329,339 - IAI47_01505 0.73 -1.8 329,368 + IAI47_01505 0.77 +0.8 329,369 - IAI47_01505 0.77 -0.6 329,436 + IAI47_01505 0.87 +0.9 329,436 + IAI47_01505 0.87 -1.0 329,436 + IAI47_01505 0.87 +0.4 329,437 - IAI47_01505 0.87 +0.9 329,437 - IAI47_01505 0.87 +2.3 329,437 - IAI47_01505 0.87 +0.0 329,437 - IAI47_01505 0.87 +1.7 329,440 + IAI47_01505 0.88 -1.9 329,440 + IAI47_01505 0.88 +0.7 329,440 + IAI47_01505 0.88 -1.4 329,440 + IAI47_01505 0.88 +1.8 329,440 + IAI47_01505 0.88 -0.5 329,440 + IAI47_01505 0.88 +1.5 329,441 - IAI47_01505 0.88 -1.6 329,441 - IAI47_01505 0.88 +0.4 329,441 - IAI47_01505 0.88 -0.7 329,441 - IAI47_01505 0.88 +0.4 329,441 - IAI47_01505 0.88 +0.2 329,521 + +0.3 329,521 + -1.4 329,521 + -1.3 329,521 + +1.4 329,521 + -2.7 329,522 - -1.8
Or see this region's nucleotide sequence