Experiment: Survival; Treatment=FW106 (pH3.9) with 1 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_07525 and IAI47_07530 are separated by 509 nucleotides IAI47_07530 and IAI47_07535 are separated by 2 nucleotides
IAI47_07525: IAI47_07525 - conjugal transfer protein TraD, at 1,587,503 to 1,587,814
_07525
IAI47_07530: IAI47_07530 - type III restriction-modification system endonuclease, at 1,588,324 to 1,591,233
_07530
IAI47_07535: IAI47_07535 - site-specific DNA-methyltransferase, at 1,591,236 to 1,593,176
_07535
Position (kb)
1588
1589
1590
1591
1592 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1587.340 kb on - strand at 1587.355 kb on + strand at 1587.499 kb on - strand at 1587.517 kb on + strand at 1587.660 kb on + strand, within IAI47_07525 at 1587.664 kb on + strand, within IAI47_07525 at 1587.665 kb on - strand, within IAI47_07525 at 1587.670 kb on + strand, within IAI47_07525 at 1587.680 kb on - strand, within IAI47_07525 at 1587.718 kb on + strand, within IAI47_07525 at 1587.723 kb on + strand, within IAI47_07525 at 1587.723 kb on + strand, within IAI47_07525 at 1587.740 kb on - strand, within IAI47_07525 at 1587.823 kb on + strand at 1587.841 kb on - strand at 1587.843 kb on - strand at 1587.895 kb on + strand at 1587.988 kb on + strand at 1588.006 kb on - strand at 1588.058 kb on + strand at 1588.119 kb on - strand at 1588.187 kb on + strand at 1588.188 kb on - strand at 1588.189 kb on + strand at 1588.189 kb on + strand at 1588.245 kb on - strand at 1588.248 kb on + strand at 1588.248 kb on + strand at 1588.249 kb on - strand at 1588.432 kb on + strand at 1588.482 kb on + strand at 1588.532 kb on + strand at 1588.533 kb on - strand at 1588.551 kb on - strand at 1588.613 kb on - strand at 1588.664 kb on + strand, within IAI47_07530 at 1588.723 kb on - strand, within IAI47_07530 at 1588.810 kb on - strand, within IAI47_07530 at 1588.858 kb on - strand, within IAI47_07530 at 1588.945 kb on + strand, within IAI47_07530 at 1588.951 kb on - strand, within IAI47_07530 at 1589.179 kb on + strand, within IAI47_07530 at 1589.221 kb on + strand, within IAI47_07530 at 1589.231 kb on - strand, within IAI47_07530 at 1589.379 kb on + strand, within IAI47_07530 at 1589.564 kb on + strand, within IAI47_07530 at 1589.606 kb on + strand, within IAI47_07530 at 1589.606 kb on + strand, within IAI47_07530 at 1589.606 kb on + strand, within IAI47_07530 at 1589.607 kb on - strand, within IAI47_07530 at 1589.638 kb on + strand, within IAI47_07530 at 1589.639 kb on - strand, within IAI47_07530 at 1589.827 kb on - strand, within IAI47_07530 at 1589.869 kb on + strand, within IAI47_07530 at 1589.870 kb on - strand, within IAI47_07530 at 1589.988 kb on + strand, within IAI47_07530 at 1590.037 kb on - strand, within IAI47_07530 at 1590.080 kb on - strand, within IAI47_07530 at 1590.177 kb on + strand, within IAI47_07530 at 1590.250 kb on - strand, within IAI47_07530 at 1590.547 kb on - strand, within IAI47_07530 at 1590.660 kb on + strand, within IAI47_07530 at 1590.692 kb on - strand, within IAI47_07530 at 1590.903 kb on + strand, within IAI47_07530 at 1590.953 kb on + strand at 1590.973 kb on - strand at 1590.998 kb on - strand at 1591.017 kb on - strand at 1591.038 kb on + strand at 1591.091 kb on - strand at 1591.123 kb on - strand at 1591.148 kb on - strand at 1591.228 kb on - strand at 1591.278 kb on - strand at 1591.413 kb on - strand at 1591.465 kb on + strand, within IAI47_07535 at 1591.473 kb on - strand, within IAI47_07535 at 1591.509 kb on - strand, within IAI47_07535 at 1591.516 kb on + strand, within IAI47_07535 at 1591.517 kb on - strand, within IAI47_07535 at 1591.517 kb on - strand, within IAI47_07535 at 1591.518 kb on + strand, within IAI47_07535 at 1591.519 kb on - strand, within IAI47_07535 at 1591.519 kb on - strand, within IAI47_07535 at 1591.519 kb on - strand, within IAI47_07535 at 1591.546 kb on + strand, within IAI47_07535 at 1591.584 kb on - strand, within IAI47_07535 at 1591.584 kb on - strand, within IAI47_07535 at 1591.599 kb on - strand, within IAI47_07535 at 1591.599 kb on - strand, within IAI47_07535 at 1591.599 kb on - strand, within IAI47_07535 at 1591.645 kb on - strand, within IAI47_07535 at 1591.823 kb on + strand, within IAI47_07535 at 1592.179 kb on + strand, within IAI47_07535 at 1592.186 kb on - strand, within IAI47_07535 at 1592.227 kb on + strand, within IAI47_07535 at 1592.227 kb on + strand, within IAI47_07535 at 1592.228 kb on - strand, within IAI47_07535
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW106 (pH3.9) with 1 mM Glucose; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 1,587,340 - +0.7 1,587,355 + +0.7 1,587,499 - -0.8 1,587,517 + +0.0 1,587,660 + IAI47_07525 0.50 -0.2 1,587,664 + IAI47_07525 0.52 +0.3 1,587,665 - IAI47_07525 0.52 -0.3 1,587,670 + IAI47_07525 0.54 +0.0 1,587,680 - IAI47_07525 0.57 -0.1 1,587,718 + IAI47_07525 0.69 -1.0 1,587,723 + IAI47_07525 0.71 -0.3 1,587,723 + IAI47_07525 0.71 -0.5 1,587,740 - IAI47_07525 0.76 +0.0 1,587,823 + +0.8 1,587,841 - +0.3 1,587,843 - +1.0 1,587,895 + -0.5 1,587,988 + +2.0 1,588,006 - -1.0 1,588,058 + -1.0 1,588,119 - -1.8 1,588,187 + +0.5 1,588,188 - -1.2 1,588,189 + +0.3 1,588,189 + -0.3 1,588,245 - +1.5 1,588,248 + +0.8 1,588,248 + -0.6 1,588,249 - +2.3 1,588,432 + -1.2 1,588,482 + -0.5 1,588,532 + +1.6 1,588,533 - +0.6 1,588,551 - -0.8 1,588,613 - +0.3 1,588,664 + IAI47_07530 0.12 +0.9 1,588,723 - IAI47_07530 0.14 -0.9 1,588,810 - IAI47_07530 0.17 -0.5 1,588,858 - IAI47_07530 0.18 -1.3 1,588,945 + IAI47_07530 0.21 -0.8 1,588,951 - IAI47_07530 0.22 +0.3 1,589,179 + IAI47_07530 0.29 +1.0 1,589,221 + IAI47_07530 0.31 -0.5 1,589,231 - IAI47_07530 0.31 +0.0 1,589,379 + IAI47_07530 0.36 -0.2 1,589,564 + IAI47_07530 0.43 -1.7 1,589,606 + IAI47_07530 0.44 -1.3 1,589,606 + IAI47_07530 0.44 -0.3 1,589,606 + IAI47_07530 0.44 +0.9 1,589,607 - IAI47_07530 0.44 -1.1 1,589,638 + IAI47_07530 0.45 +3.6 1,589,639 - IAI47_07530 0.45 -1.0 1,589,827 - IAI47_07530 0.52 +4.1 1,589,869 + IAI47_07530 0.53 +0.6 1,589,870 - IAI47_07530 0.53 -1.0 1,589,988 + IAI47_07530 0.57 +0.3 1,590,037 - IAI47_07530 0.59 -0.7 1,590,080 - IAI47_07530 0.60 +0.6 1,590,177 + IAI47_07530 0.64 -1.3 1,590,250 - IAI47_07530 0.66 +1.8 1,590,547 - IAI47_07530 0.76 +1.6 1,590,660 + IAI47_07530 0.80 -1.8 1,590,692 - IAI47_07530 0.81 -1.4 1,590,903 + IAI47_07530 0.89 +0.0 1,590,953 + +0.2 1,590,973 - -0.3 1,590,998 - +0.8 1,591,017 - -0.5 1,591,038 + +0.5 1,591,091 - +1.5 1,591,123 - -2.0 1,591,148 - -0.4 1,591,228 - -1.8 1,591,278 - -1.0 1,591,413 - +2.0 1,591,465 + IAI47_07535 0.12 +2.6 1,591,473 - IAI47_07535 0.12 +0.4 1,591,509 - IAI47_07535 0.14 -1.5 1,591,516 + IAI47_07535 0.14 +0.1 1,591,517 - IAI47_07535 0.14 -0.3 1,591,517 - IAI47_07535 0.14 +4.2 1,591,518 + IAI47_07535 0.15 -1.5 1,591,519 - IAI47_07535 0.15 -1.3 1,591,519 - IAI47_07535 0.15 +0.0 1,591,519 - IAI47_07535 0.15 -0.6 1,591,546 + IAI47_07535 0.16 +0.5 1,591,584 - IAI47_07535 0.18 +2.2 1,591,584 - IAI47_07535 0.18 +0.0 1,591,599 - IAI47_07535 0.19 +4.9 1,591,599 - IAI47_07535 0.19 -0.4 1,591,599 - IAI47_07535 0.19 +2.6 1,591,645 - IAI47_07535 0.21 +0.8 1,591,823 + IAI47_07535 0.30 -0.7 1,592,179 + IAI47_07535 0.49 -0.4 1,592,186 - IAI47_07535 0.49 +2.0 1,592,227 + IAI47_07535 0.51 +0.8 1,592,227 + IAI47_07535 0.51 -1.4 1,592,228 - IAI47_07535 0.51 +0.5
Or see this region's nucleotide sequence