Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20640 and IAI47_20645 are separated by 165 nucleotides IAI47_20645 and IAI47_20650 are separated by 219 nucleotides IAI47_20650 and IAI47_20655 are separated by 9 nucleotides
IAI47_20640: IAI47_20640 - hypothetical protein, at 378,590 to 378,817
_20640
IAI47_20645: IAI47_20645 - YtfJ family protein, at 378,983 to 379,513
_20645
IAI47_20650: IAI47_20650 - polyprenyl synthetase family protein, at 379,733 to 380,641
_20650
IAI47_20655: IAI47_20655 - glycosyltransferase family 1 protein, at 380,651 to 381,946
_20655
Position (kb) 379
380
381 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 378.775 kb on + strand, within IAI47_20640 at 378.775 kb on + strand, within IAI47_20640 at 378.775 kb on + strand, within IAI47_20640 at 378.775 kb on + strand, within IAI47_20640 at 378.776 kb on - strand, within IAI47_20640 at 378.776 kb on - strand, within IAI47_20640 at 378.776 kb on - strand, within IAI47_20640 at 378.776 kb on - strand, within IAI47_20640 at 378.776 kb on - strand, within IAI47_20640 at 378.777 kb on + strand, within IAI47_20640 at 378.778 kb on - strand, within IAI47_20640 at 378.794 kb on + strand, within IAI47_20640 at 378.795 kb on - strand at 378.811 kb on + strand at 378.812 kb on - strand at 378.831 kb on + strand at 378.867 kb on + strand at 378.868 kb on - strand at 378.892 kb on + strand at 378.892 kb on + strand at 378.893 kb on - strand at 378.893 kb on - strand at 378.901 kb on + strand at 378.904 kb on + strand at 378.904 kb on + strand at 378.904 kb on + strand at 378.905 kb on - strand at 378.905 kb on - strand at 378.905 kb on - strand at 378.927 kb on + strand at 378.927 kb on + strand at 378.927 kb on + strand at 378.927 kb on + strand at 378.927 kb on + strand at 378.927 kb on + strand at 378.928 kb on - strand at 378.928 kb on - strand at 378.928 kb on - strand at 378.928 kb on - strand at 378.936 kb on + strand at 378.936 kb on + strand at 378.937 kb on - strand at 378.937 kb on - strand at 378.940 kb on + strand at 378.940 kb on + strand at 378.940 kb on + strand at 378.941 kb on - strand at 378.941 kb on - strand at 378.941 kb on - strand at 378.988 kb on - strand at 378.988 kb on - strand at 379.055 kb on + strand, within IAI47_20645 at 379.055 kb on + strand, within IAI47_20645 at 379.055 kb on + strand, within IAI47_20645 at 379.055 kb on + strand, within IAI47_20645 at 379.055 kb on + strand, within IAI47_20645 at 379.056 kb on - strand, within IAI47_20645 at 379.056 kb on - strand, within IAI47_20645 at 379.056 kb on - strand, within IAI47_20645 at 379.103 kb on + strand, within IAI47_20645 at 379.104 kb on - strand, within IAI47_20645 at 379.197 kb on + strand, within IAI47_20645 at 379.197 kb on + strand, within IAI47_20645 at 379.197 kb on + strand, within IAI47_20645 at 379.262 kb on - strand, within IAI47_20645 at 379.297 kb on - strand, within IAI47_20645 at 379.303 kb on + strand, within IAI47_20645 at 379.303 kb on + strand, within IAI47_20645 at 379.303 kb on + strand, within IAI47_20645 at 379.303 kb on + strand, within IAI47_20645 at 379.304 kb on - strand, within IAI47_20645 at 379.304 kb on - strand, within IAI47_20645 at 379.304 kb on - strand, within IAI47_20645 at 379.304 kb on - strand, within IAI47_20645 at 379.304 kb on - strand, within IAI47_20645 at 379.349 kb on - strand, within IAI47_20645 at 379.458 kb on + strand, within IAI47_20645 at 379.458 kb on + strand, within IAI47_20645 at 379.458 kb on + strand, within IAI47_20645 at 379.459 kb on - strand, within IAI47_20645 at 379.533 kb on + strand at 379.533 kb on + strand at 379.533 kb on + strand at 379.534 kb on - strand at 379.538 kb on - strand at 379.538 kb on - strand at 379.581 kb on + strand at 379.594 kb on + strand at 379.595 kb on - strand at 379.600 kb on + strand at 379.600 kb on + strand at 379.600 kb on + strand at 379.601 kb on - strand at 379.601 kb on - strand at 379.601 kb on - strand at 379.606 kb on + strand at 379.606 kb on + strand at 379.607 kb on - strand at 379.715 kb on + strand at 379.715 kb on + strand at 379.716 kb on - strand at 379.716 kb on - strand at 379.720 kb on - strand at 379.720 kb on - strand at 379.720 kb on - strand at 379.824 kb on + strand, within IAI47_20650 at 379.905 kb on + strand, within IAI47_20650 at 379.906 kb on - strand, within IAI47_20650 at 380.075 kb on + strand, within IAI47_20650 at 380.076 kb on - strand, within IAI47_20650 at 380.119 kb on + strand, within IAI47_20650 at 380.202 kb on + strand, within IAI47_20650 at 380.202 kb on + strand, within IAI47_20650 at 380.203 kb on - strand, within IAI47_20650 at 380.206 kb on + strand, within IAI47_20650 at 380.206 kb on + strand, within IAI47_20650 at 380.207 kb on - strand, within IAI47_20650 at 380.207 kb on - strand, within IAI47_20650 at 380.207 kb on - strand, within IAI47_20650 at 380.296 kb on + strand, within IAI47_20650 at 380.296 kb on + strand, within IAI47_20650 at 380.296 kb on + strand, within IAI47_20650 at 380.297 kb on - strand, within IAI47_20650 at 380.297 kb on - strand, within IAI47_20650 at 380.428 kb on + strand, within IAI47_20650 at 380.428 kb on + strand, within IAI47_20650 at 380.428 kb on + strand, within IAI47_20650 at 380.476 kb on + strand, within IAI47_20650 at 380.477 kb on - strand, within IAI47_20650 at 380.582 kb on + strand at 380.639 kb on + strand at 380.640 kb on - strand at 380.640 kb on - strand at 380.661 kb on - strand at 380.661 kb on - strand at 380.684 kb on + strand at 380.684 kb on + strand at 380.685 kb on - strand at 380.686 kb on + strand at 380.713 kb on + strand at 380.714 kb on - strand at 380.714 kb on - strand at 380.724 kb on + strand at 380.746 kb on + strand at 380.746 kb on + strand at 380.747 kb on - strand at 380.747 kb on - strand at 380.747 kb on - strand at 380.923 kb on + strand, within IAI47_20655 at 380.924 kb on - strand, within IAI47_20655 at 380.924 kb on - strand, within IAI47_20655 at 381.009 kb on + strand, within IAI47_20655 at 381.010 kb on - strand, within IAI47_20655 at 381.010 kb on - strand, within IAI47_20655 at 381.107 kb on + strand, within IAI47_20655 at 381.108 kb on - strand, within IAI47_20655 at 381.108 kb on - strand, within IAI47_20655 at 381.220 kb on - strand, within IAI47_20655 at 381.278 kb on - strand, within IAI47_20655 at 381.380 kb on + strand, within IAI47_20655 at 381.381 kb on - strand, within IAI47_20655 at 381.381 kb on - strand, within IAI47_20655 at 381.381 kb on - strand, within IAI47_20655 at 381.454 kb on + strand, within IAI47_20655 at 381.496 kb on - strand, within IAI47_20655
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 378,775 + IAI47_20640 0.81 -1.1 378,775 + IAI47_20640 0.81 -1.0 378,775 + IAI47_20640 0.81 -0.7 378,775 + IAI47_20640 0.81 -0.9 378,776 - IAI47_20640 0.82 -0.5 378,776 - IAI47_20640 0.82 +1.3 378,776 - IAI47_20640 0.82 -1.9 378,776 - IAI47_20640 0.82 +0.2 378,776 - IAI47_20640 0.82 -2.3 378,777 + IAI47_20640 0.82 +0.3 378,778 - IAI47_20640 0.82 +2.2 378,794 + IAI47_20640 0.89 +1.0 378,795 - +0.8 378,811 + -0.0 378,812 - -1.1 378,831 + +2.3 378,867 + -1.6 378,868 - -1.0 378,892 + +1.6 378,892 + +0.4 378,893 - +0.5 378,893 - -0.3 378,901 + +0.7 378,904 + +0.3 378,904 + +0.7 378,904 + -2.2 378,905 - -1.7 378,905 - -0.3 378,905 - -0.7 378,927 + -0.3 378,927 + +0.2 378,927 + -0.4 378,927 + +1.3 378,927 + +0.0 378,927 + +0.3 378,928 - -0.3 378,928 - -0.1 378,928 - +1.4 378,928 - +1.2 378,936 + -0.2 378,936 + +0.3 378,937 - +0.8 378,937 - -1.3 378,940 + +0.2 378,940 + -0.4 378,940 + +0.7 378,941 - +1.9 378,941 - -1.2 378,941 - -0.0 378,988 - +0.4 378,988 - +0.6 379,055 + IAI47_20645 0.14 +1.2 379,055 + IAI47_20645 0.14 -1.3 379,055 + IAI47_20645 0.14 +1.2 379,055 + IAI47_20645 0.14 -1.3 379,055 + IAI47_20645 0.14 -0.1 379,056 - IAI47_20645 0.14 -0.6 379,056 - IAI47_20645 0.14 +0.7 379,056 - IAI47_20645 0.14 +1.1 379,103 + IAI47_20645 0.23 -2.0 379,104 - IAI47_20645 0.23 +0.2 379,197 + IAI47_20645 0.40 -1.0 379,197 + IAI47_20645 0.40 -1.2 379,197 + IAI47_20645 0.40 +3.3 379,262 - IAI47_20645 0.53 +1.1 379,297 - IAI47_20645 0.59 +1.0 379,303 + IAI47_20645 0.60 +1.3 379,303 + IAI47_20645 0.60 +0.3 379,303 + IAI47_20645 0.60 +1.2 379,303 + IAI47_20645 0.60 +0.8 379,304 - IAI47_20645 0.60 -1.4 379,304 - IAI47_20645 0.60 +0.2 379,304 - IAI47_20645 0.60 -0.4 379,304 - IAI47_20645 0.60 -1.4 379,304 - IAI47_20645 0.60 +1.2 379,349 - IAI47_20645 0.69 -0.1 379,458 + IAI47_20645 0.89 -0.1 379,458 + IAI47_20645 0.89 +0.8 379,458 + IAI47_20645 0.89 +1.9 379,459 - IAI47_20645 0.90 -0.1 379,533 + +1.1 379,533 + +1.6 379,533 + +0.4 379,534 - +1.4 379,538 - -0.9 379,538 - +3.1 379,581 + +0.7 379,594 + -1.7 379,595 - +0.5 379,600 + -0.8 379,600 + +0.1 379,600 + +1.5 379,601 - +0.1 379,601 - +0.2 379,601 - -0.9 379,606 + +1.2 379,606 + -1.0 379,607 - +0.7 379,715 + -0.5 379,715 + -0.0 379,716 - -2.0 379,716 - +1.3 379,720 - -0.6 379,720 - -0.9 379,720 - +1.7 379,824 + IAI47_20650 0.10 +1.3 379,905 + IAI47_20650 0.19 -1.8 379,906 - IAI47_20650 0.19 +0.4 380,075 + IAI47_20650 0.38 -0.4 380,076 - IAI47_20650 0.38 -0.8 380,119 + IAI47_20650 0.42 -1.0 380,202 + IAI47_20650 0.52 +0.4 380,202 + IAI47_20650 0.52 +0.2 380,203 - IAI47_20650 0.52 -0.3 380,206 + IAI47_20650 0.52 +1.5 380,206 + IAI47_20650 0.52 +0.5 380,207 - IAI47_20650 0.52 +0.9 380,207 - IAI47_20650 0.52 +0.3 380,207 - IAI47_20650 0.52 +1.5 380,296 + IAI47_20650 0.62 +0.6 380,296 + IAI47_20650 0.62 -2.5 380,296 + IAI47_20650 0.62 -1.7 380,297 - IAI47_20650 0.62 -0.8 380,297 - IAI47_20650 0.62 -0.9 380,428 + IAI47_20650 0.76 -1.0 380,428 + IAI47_20650 0.76 -2.3 380,428 + IAI47_20650 0.76 -0.7 380,476 + IAI47_20650 0.82 +0.4 380,477 - IAI47_20650 0.82 -0.5 380,582 + +1.2 380,639 + +0.3 380,640 - +0.7 380,640 - +1.1 380,661 - -0.0 380,661 - +1.6 380,684 + -0.0 380,684 + -2.0 380,685 - -1.5 380,686 + -1.1 380,713 + -0.4 380,714 - +1.9 380,714 - -0.0 380,724 + +0.6 380,746 + +1.0 380,746 + -1.1 380,747 - +1.6 380,747 - -0.1 380,747 - -1.2 380,923 + IAI47_20655 0.21 +0.8 380,924 - IAI47_20655 0.21 +2.0 380,924 - IAI47_20655 0.21 -0.8 381,009 + IAI47_20655 0.28 -0.3 381,010 - IAI47_20655 0.28 -0.3 381,010 - IAI47_20655 0.28 +0.4 381,107 + IAI47_20655 0.35 +1.7 381,108 - IAI47_20655 0.35 +2.2 381,108 - IAI47_20655 0.35 -1.7 381,220 - IAI47_20655 0.44 -0.8 381,278 - IAI47_20655 0.48 +1.0 381,380 + IAI47_20655 0.56 -0.2 381,381 - IAI47_20655 0.56 +1.6 381,381 - IAI47_20655 0.56 +0.3 381,381 - IAI47_20655 0.56 +0.6 381,454 + IAI47_20655 0.62 +0.5 381,496 - IAI47_20655 0.65 +0.3
Or see this region's nucleotide sequence