Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_04160 and IAI47_04165 are separated by 60 nucleotides IAI47_04165 and IAI47_04170 are separated by 9 nucleotides IAI47_04170 and IAI47_04175 are separated by 364 nucleotides
IAI47_04160: IAI47_04160 - phosphoadenylyl-sulfate reductase, at 875,449 to 876,183
_04160
IAI47_04165: IAI47_04165 - hypothetical protein, at 876,244 to 876,528
_04165
IAI47_04170: IAI47_04170 - aminopeptidase, at 876,538 to 877,551
_04170
IAI47_04175: IAI47_04175 - uroporphyrinogen-III C-methyltransferase, at 877,916 to 879,331
_04175
Position (kb) 876
877
878 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 875.590 kb on + strand, within IAI47_04160 at 875.590 kb on + strand, within IAI47_04160 at 875.591 kb on - strand, within IAI47_04160 at 875.591 kb on - strand, within IAI47_04160 at 875.591 kb on - strand, within IAI47_04160 at 875.591 kb on - strand, within IAI47_04160 at 875.707 kb on + strand, within IAI47_04160 at 875.707 kb on + strand, within IAI47_04160 at 875.707 kb on + strand, within IAI47_04160 at 875.707 kb on + strand, within IAI47_04160 at 875.707 kb on + strand, within IAI47_04160 at 875.708 kb on - strand, within IAI47_04160 at 875.708 kb on - strand, within IAI47_04160 at 875.708 kb on - strand, within IAI47_04160 at 875.715 kb on + strand, within IAI47_04160 at 875.715 kb on + strand, within IAI47_04160 at 875.716 kb on - strand, within IAI47_04160 at 875.716 kb on - strand, within IAI47_04160 at 875.716 kb on - strand, within IAI47_04160 at 875.839 kb on + strand, within IAI47_04160 at 875.839 kb on + strand, within IAI47_04160 at 875.839 kb on + strand, within IAI47_04160 at 875.839 kb on + strand, within IAI47_04160 at 875.839 kb on + strand, within IAI47_04160 at 875.839 kb on + strand, within IAI47_04160 at 875.839 kb on + strand, within IAI47_04160 at 875.840 kb on + strand, within IAI47_04160 at 875.840 kb on - strand, within IAI47_04160 at 875.840 kb on - strand, within IAI47_04160 at 875.842 kb on - strand, within IAI47_04160 at 875.842 kb on - strand, within IAI47_04160 at 875.850 kb on + strand, within IAI47_04160 at 875.963 kb on + strand, within IAI47_04160 at 875.963 kb on + strand, within IAI47_04160 at 875.964 kb on - strand, within IAI47_04160 at 875.964 kb on - strand, within IAI47_04160 at 875.980 kb on - strand, within IAI47_04160 at 875.980 kb on - strand, within IAI47_04160 at 875.980 kb on - strand, within IAI47_04160 at 876.007 kb on - strand, within IAI47_04160 at 876.026 kb on - strand, within IAI47_04160 at 876.045 kb on + strand, within IAI47_04160 at 876.045 kb on + strand, within IAI47_04160 at 876.045 kb on + strand, within IAI47_04160 at 876.045 kb on + strand, within IAI47_04160 at 876.045 kb on + strand, within IAI47_04160 at 876.045 kb on + strand, within IAI47_04160 at 876.045 kb on + strand, within IAI47_04160 at 876.045 kb on + strand, within IAI47_04160 at 876.045 kb on + strand, within IAI47_04160 at 876.045 kb on + strand, within IAI47_04160 at 876.045 kb on + strand, within IAI47_04160 at 876.045 kb on + strand, within IAI47_04160 at 876.045 kb on + strand, within IAI47_04160 at 876.045 kb on + strand, within IAI47_04160 at 876.045 kb on + strand, within IAI47_04160 at 876.045 kb on + strand, within IAI47_04160 at 876.046 kb on - strand, within IAI47_04160 at 876.046 kb on - strand, within IAI47_04160 at 876.046 kb on - strand, within IAI47_04160 at 876.046 kb on - strand, within IAI47_04160 at 876.046 kb on - strand, within IAI47_04160 at 876.046 kb on - strand, within IAI47_04160 at 876.046 kb on - strand, within IAI47_04160 at 876.046 kb on - strand, within IAI47_04160 at 876.046 kb on - strand, within IAI47_04160 at 876.046 kb on - strand, within IAI47_04160 at 876.046 kb on - strand, within IAI47_04160 at 876.046 kb on - strand, within IAI47_04160 at 876.046 kb on - strand, within IAI47_04160 at 876.046 kb on - strand, within IAI47_04160 at 876.046 kb on - strand, within IAI47_04160 at 876.046 kb on - strand, within IAI47_04160 at 876.046 kb on - strand, within IAI47_04160 at 876.049 kb on + strand, within IAI47_04160 at 876.049 kb on + strand, within IAI47_04160 at 876.049 kb on + strand, within IAI47_04160 at 876.050 kb on - strand, within IAI47_04160 at 876.050 kb on - strand, within IAI47_04160 at 876.050 kb on - strand, within IAI47_04160 at 876.073 kb on + strand, within IAI47_04160 at 876.074 kb on - strand, within IAI47_04160 at 876.182 kb on - strand at 876.182 kb on - strand at 876.182 kb on - strand at 876.182 kb on - strand at 876.182 kb on - strand at 876.216 kb on - strand at 876.245 kb on + strand at 876.245 kb on + strand at 876.256 kb on + strand at 876.257 kb on - strand at 876.308 kb on + strand, within IAI47_04165 at 876.308 kb on + strand, within IAI47_04165 at 876.308 kb on + strand, within IAI47_04165 at 876.308 kb on + strand, within IAI47_04165 at 876.308 kb on + strand, within IAI47_04165 at 876.308 kb on + strand, within IAI47_04165 at 876.309 kb on - strand, within IAI47_04165 at 876.309 kb on - strand, within IAI47_04165 at 876.309 kb on - strand, within IAI47_04165 at 876.309 kb on - strand, within IAI47_04165 at 876.408 kb on + strand, within IAI47_04165 at 876.408 kb on + strand, within IAI47_04165 at 876.408 kb on + strand, within IAI47_04165 at 876.408 kb on + strand, within IAI47_04165 at 876.409 kb on - strand, within IAI47_04165 at 876.409 kb on - strand, within IAI47_04165 at 876.409 kb on - strand, within IAI47_04165 at 876.424 kb on - strand, within IAI47_04165 at 876.424 kb on - strand, within IAI47_04165 at 876.443 kb on + strand, within IAI47_04165 at 876.463 kb on - strand, within IAI47_04165 at 876.463 kb on - strand, within IAI47_04165 at 876.463 kb on - strand, within IAI47_04165 at 876.506 kb on + strand at 876.506 kb on + strand at 876.506 kb on + strand at 876.507 kb on - strand at 876.507 kb on - strand at 876.507 kb on - strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.524 kb on + strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.525 kb on - strand at 876.611 kb on - strand at 876.638 kb on + strand at 876.639 kb on - strand at 876.639 kb on - strand at 876.649 kb on - strand, within IAI47_04170 at 876.649 kb on - strand, within IAI47_04170 at 876.709 kb on + strand, within IAI47_04170 at 876.710 kb on - strand, within IAI47_04170 at 876.727 kb on - strand, within IAI47_04170 at 876.741 kb on + strand, within IAI47_04170 at 876.751 kb on - strand, within IAI47_04170 at 876.751 kb on - strand, within IAI47_04170 at 876.751 kb on - strand, within IAI47_04170 at 876.856 kb on + strand, within IAI47_04170 at 876.856 kb on + strand, within IAI47_04170 at 876.856 kb on + strand, within IAI47_04170 at 876.856 kb on + strand, within IAI47_04170 at 876.857 kb on - strand, within IAI47_04170 at 876.857 kb on - strand, within IAI47_04170 at 876.920 kb on + strand, within IAI47_04170 at 876.920 kb on + strand, within IAI47_04170 at 876.921 kb on - strand, within IAI47_04170 at 876.937 kb on + strand, within IAI47_04170 at 876.945 kb on + strand, within IAI47_04170 at 876.946 kb on - strand, within IAI47_04170 at 877.004 kb on + strand, within IAI47_04170 at 877.004 kb on + strand, within IAI47_04170 at 877.004 kb on + strand, within IAI47_04170 at 877.005 kb on - strand, within IAI47_04170 at 877.005 kb on - strand, within IAI47_04170 at 877.005 kb on - strand, within IAI47_04170 at 877.005 kb on - strand, within IAI47_04170 at 877.005 kb on - strand, within IAI47_04170 at 877.005 kb on - strand, within IAI47_04170 at 877.005 kb on - strand, within IAI47_04170 at 877.005 kb on - strand, within IAI47_04170 at 877.005 kb on - strand, within IAI47_04170 at 877.064 kb on + strand, within IAI47_04170 at 877.064 kb on + strand, within IAI47_04170 at 877.065 kb on - strand, within IAI47_04170 at 877.150 kb on + strand, within IAI47_04170 at 877.151 kb on - strand, within IAI47_04170 at 877.151 kb on - strand, within IAI47_04170 at 877.201 kb on - strand, within IAI47_04170 at 877.278 kb on + strand, within IAI47_04170 at 877.279 kb on - strand, within IAI47_04170 at 877.279 kb on - strand, within IAI47_04170 at 877.347 kb on + strand, within IAI47_04170 at 877.347 kb on + strand, within IAI47_04170 at 877.348 kb on - strand, within IAI47_04170 at 877.349 kb on + strand, within IAI47_04170 at 877.362 kb on + strand, within IAI47_04170 at 877.362 kb on + strand, within IAI47_04170 at 877.363 kb on - strand, within IAI47_04170 at 877.379 kb on + strand, within IAI47_04170 at 877.379 kb on + strand, within IAI47_04170 at 877.379 kb on + strand, within IAI47_04170 at 877.379 kb on + strand, within IAI47_04170 at 877.380 kb on - strand, within IAI47_04170 at 877.380 kb on - strand, within IAI47_04170 at 877.380 kb on - strand, within IAI47_04170 at 877.380 kb on - strand, within IAI47_04170 at 877.380 kb on - strand, within IAI47_04170 at 877.430 kb on + strand, within IAI47_04170 at 877.430 kb on + strand, within IAI47_04170 at 877.431 kb on - strand, within IAI47_04170 at 877.483 kb on + strand at 877.510 kb on - strand at 877.551 kb on + strand at 877.552 kb on - strand at 877.552 kb on - strand at 877.570 kb on + strand at 877.570 kb on + strand at 877.618 kb on + strand at 877.618 kb on + strand at 877.618 kb on + strand at 877.619 kb on - strand at 877.619 kb on - strand at 877.619 kb on - strand at 877.619 kb on - strand at 877.621 kb on + strand at 877.621 kb on + strand at 877.621 kb on + strand at 877.621 kb on + strand at 877.622 kb on - strand at 877.622 kb on - strand at 877.622 kb on - strand at 877.622 kb on - strand at 877.622 kb on - strand at 877.623 kb on + strand at 877.623 kb on + strand at 877.623 kb on + strand at 877.623 kb on + strand at 877.623 kb on + strand at 877.624 kb on - strand at 877.624 kb on - strand at 877.624 kb on - strand at 877.624 kb on - strand at 877.624 kb on - strand at 877.657 kb on - strand at 877.657 kb on - strand at 877.693 kb on + strand at 877.694 kb on - strand at 877.706 kb on + strand at 877.706 kb on + strand at 877.706 kb on + strand at 877.706 kb on + strand at 877.706 kb on + strand at 877.707 kb on - strand at 877.707 kb on - strand at 877.707 kb on - strand at 877.716 kb on + strand at 877.716 kb on + strand at 877.716 kb on + strand at 877.716 kb on + strand at 877.716 kb on + strand at 877.717 kb on - strand at 877.718 kb on - strand at 877.723 kb on - strand at 877.723 kb on - strand at 877.723 kb on - strand at 877.769 kb on - strand at 877.769 kb on - strand at 877.769 kb on - strand at 877.770 kb on + strand at 877.770 kb on + strand at 877.770 kb on + strand at 877.770 kb on + strand at 877.771 kb on - strand at 877.771 kb on - strand at 877.771 kb on - strand at 877.771 kb on - strand at 877.771 kb on - strand at 877.771 kb on - strand at 877.771 kb on - strand at 877.782 kb on + strand at 877.782 kb on + strand at 877.782 kb on + strand at 877.782 kb on + strand at 877.782 kb on + strand at 877.783 kb on - strand at 877.816 kb on + strand at 877.817 kb on - strand at 877.832 kb on + strand at 877.833 kb on - strand at 877.862 kb on + strand at 877.862 kb on + strand at 877.863 kb on - strand at 877.876 kb on + strand at 877.876 kb on + strand at 877.876 kb on + strand at 877.877 kb on - strand at 877.877 kb on - strand at 877.877 kb on - strand at 877.877 kb on - strand at 877.878 kb on + strand at 877.878 kb on + strand at 877.879 kb on - strand at 877.879 kb on - strand at 877.888 kb on - strand at 877.890 kb on + strand at 877.890 kb on + strand at 877.891 kb on - strand at 877.913 kb on - strand at 877.922 kb on + strand at 877.922 kb on + strand at 877.922 kb on + strand at 877.922 kb on + strand at 877.923 kb on - strand at 877.923 kb on - strand at 877.923 kb on - strand at 877.982 kb on - strand at 877.982 kb on - strand at 877.982 kb on - strand at 877.982 kb on - strand at 878.005 kb on - strand at 878.005 kb on - strand at 878.248 kb on + strand, within IAI47_04175 at 878.248 kb on + strand, within IAI47_04175 at 878.248 kb on + strand, within IAI47_04175 at 878.249 kb on - strand, within IAI47_04175 at 878.249 kb on - strand, within IAI47_04175 at 878.249 kb on - strand, within IAI47_04175 at 878.249 kb on - strand, within IAI47_04175 at 878.249 kb on - strand, within IAI47_04175 at 878.249 kb on - strand, within IAI47_04175 at 878.326 kb on + strand, within IAI47_04175 at 878.326 kb on + strand, within IAI47_04175 at 878.326 kb on + strand, within IAI47_04175 at 878.326 kb on + strand, within IAI47_04175 at 878.326 kb on + strand, within IAI47_04175 at 878.327 kb on - strand, within IAI47_04175 at 878.327 kb on - strand, within IAI47_04175 at 878.327 kb on - strand, within IAI47_04175 at 878.327 kb on - strand, within IAI47_04175 at 878.327 kb on - strand, within IAI47_04175 at 878.327 kb on - strand, within IAI47_04175 at 878.404 kb on + strand, within IAI47_04175 at 878.410 kb on + strand, within IAI47_04175 at 878.410 kb on + strand, within IAI47_04175 at 878.411 kb on - strand, within IAI47_04175 at 878.518 kb on + strand, within IAI47_04175 at 878.519 kb on - strand, within IAI47_04175
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 875,590 + IAI47_04160 0.19 -1.5 875,590 + IAI47_04160 0.19 -0.7 875,591 - IAI47_04160 0.19 +0.5 875,591 - IAI47_04160 0.19 -0.6 875,591 - IAI47_04160 0.19 -0.9 875,591 - IAI47_04160 0.19 +1.9 875,707 + IAI47_04160 0.35 -1.3 875,707 + IAI47_04160 0.35 +0.1 875,707 + IAI47_04160 0.35 -2.0 875,707 + IAI47_04160 0.35 -1.5 875,707 + IAI47_04160 0.35 -0.8 875,708 - IAI47_04160 0.35 -0.0 875,708 - IAI47_04160 0.35 +2.2 875,708 - IAI47_04160 0.35 +0.7 875,715 + IAI47_04160 0.36 -0.7 875,715 + IAI47_04160 0.36 +0.7 875,716 - IAI47_04160 0.36 +2.1 875,716 - IAI47_04160 0.36 -2.4 875,716 - IAI47_04160 0.36 -1.2 875,839 + IAI47_04160 0.53 -0.3 875,839 + IAI47_04160 0.53 +0.6 875,839 + IAI47_04160 0.53 -1.8 875,839 + IAI47_04160 0.53 -0.1 875,839 + IAI47_04160 0.53 +0.5 875,839 + IAI47_04160 0.53 +0.8 875,839 + IAI47_04160 0.53 +0.1 875,840 + IAI47_04160 0.53 -1.4 875,840 - IAI47_04160 0.53 -0.1 875,840 - IAI47_04160 0.53 -0.2 875,842 - IAI47_04160 0.53 +2.0 875,842 - IAI47_04160 0.53 -0.2 875,850 + IAI47_04160 0.55 -0.1 875,963 + IAI47_04160 0.70 +1.8 875,963 + IAI47_04160 0.70 -0.7 875,964 - IAI47_04160 0.70 +0.8 875,964 - IAI47_04160 0.70 -1.3 875,980 - IAI47_04160 0.72 -0.6 875,980 - IAI47_04160 0.72 -0.2 875,980 - IAI47_04160 0.72 +0.3 876,007 - IAI47_04160 0.76 +1.2 876,026 - IAI47_04160 0.79 -2.8 876,045 + IAI47_04160 0.81 +1.7 876,045 + IAI47_04160 0.81 -0.4 876,045 + IAI47_04160 0.81 -0.7 876,045 + IAI47_04160 0.81 -0.0 876,045 + IAI47_04160 0.81 +0.1 876,045 + IAI47_04160 0.81 +0.2 876,045 + IAI47_04160 0.81 -0.0 876,045 + IAI47_04160 0.81 +0.2 876,045 + IAI47_04160 0.81 +0.5 876,045 + IAI47_04160 0.81 -0.7 876,045 + IAI47_04160 0.81 -0.1 876,045 + IAI47_04160 0.81 +0.5 876,045 + IAI47_04160 0.81 -1.0 876,045 + IAI47_04160 0.81 -0.3 876,045 + IAI47_04160 0.81 +0.8 876,045 + IAI47_04160 0.81 -1.9 876,046 - IAI47_04160 0.81 +0.3 876,046 - IAI47_04160 0.81 +3.2 876,046 - IAI47_04160 0.81 -0.2 876,046 - IAI47_04160 0.81 -1.1 876,046 - IAI47_04160 0.81 +1.7 876,046 - IAI47_04160 0.81 +1.7 876,046 - IAI47_04160 0.81 -0.8 876,046 - IAI47_04160 0.81 +0.0 876,046 - IAI47_04160 0.81 -2.0 876,046 - IAI47_04160 0.81 +0.2 876,046 - IAI47_04160 0.81 -1.4 876,046 - IAI47_04160 0.81 -0.7 876,046 - IAI47_04160 0.81 +0.2 876,046 - IAI47_04160 0.81 -0.3 876,046 - IAI47_04160 0.81 +0.9 876,046 - IAI47_04160 0.81 -3.1 876,046 - IAI47_04160 0.81 -0.4 876,049 + IAI47_04160 0.82 +3.9 876,049 + IAI47_04160 0.82 +0.3 876,049 + IAI47_04160 0.82 +2.4 876,050 - IAI47_04160 0.82 +0.4 876,050 - IAI47_04160 0.82 -0.4 876,050 - IAI47_04160 0.82 +0.9 876,073 + IAI47_04160 0.85 -1.4 876,074 - IAI47_04160 0.85 -2.6 876,182 - +1.9 876,182 - -0.3 876,182 - -1.0 876,182 - +0.7 876,182 - -0.2 876,216 - +0.0 876,245 + -2.3 876,245 + +0.9 876,256 + +0.3 876,257 - -0.9 876,308 + IAI47_04165 0.22 -0.3 876,308 + IAI47_04165 0.22 -0.7 876,308 + IAI47_04165 0.22 +2.3 876,308 + IAI47_04165 0.22 -1.1 876,308 + IAI47_04165 0.22 -2.0 876,308 + IAI47_04165 0.22 +0.0 876,309 - IAI47_04165 0.23 +0.0 876,309 - IAI47_04165 0.23 +2.5 876,309 - IAI47_04165 0.23 +0.2 876,309 - IAI47_04165 0.23 -1.0 876,408 + IAI47_04165 0.58 -1.7 876,408 + IAI47_04165 0.58 -1.4 876,408 + IAI47_04165 0.58 +1.0 876,408 + IAI47_04165 0.58 +0.4 876,409 - IAI47_04165 0.58 +0.6 876,409 - IAI47_04165 0.58 -0.2 876,409 - IAI47_04165 0.58 -1.2 876,424 - IAI47_04165 0.63 +2.4 876,424 - IAI47_04165 0.63 -0.3 876,443 + IAI47_04165 0.70 +0.4 876,463 - IAI47_04165 0.77 +2.5 876,463 - IAI47_04165 0.77 -0.3 876,463 - IAI47_04165 0.77 +0.7 876,506 + -0.6 876,506 + +0.6 876,506 + +3.0 876,507 - +1.3 876,507 - +0.3 876,507 - +0.0 876,524 + +0.1 876,524 + +0.5 876,524 + -1.7 876,524 + +0.3 876,524 + -0.8 876,524 + +0.7 876,524 + -0.3 876,524 + +0.2 876,524 + -2.0 876,524 + +1.9 876,524 + -0.7 876,524 + -1.3 876,524 + -0.4 876,524 + +0.6 876,524 + +0.9 876,524 + +1.0 876,524 + -0.3 876,524 + -0.7 876,524 + +0.3 876,524 + +2.0 876,524 + +2.5 876,524 + +0.9 876,524 + +1.6 876,524 + +1.7 876,524 + +0.3 876,524 + -0.9 876,524 + +0.8 876,524 + -0.3 876,524 + -0.1 876,524 + +0.8 876,524 + +0.5 876,524 + -1.2 876,524 + +0.3 876,524 + +0.8 876,524 + -0.4 876,524 + +0.6 876,524 + +1.0 876,524 + +0.2 876,524 + +0.8 876,524 + -1.6 876,524 + +0.8 876,524 + +0.6 876,524 + +1.4 876,525 - 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Or see this region's nucleotide sequence