Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_01010 and IAI47_01015 are separated by 330 nucleotides IAI47_01015 and IAI47_01020 are separated by 150 nucleotides
IAI47_01010: IAI47_01010 - YifB family Mg chelatase-like AAA ATPase, at 213,915 to 215,435
_01010
IAI47_01015: IAI47_01015 - ilv operon leader peptide, at 215,766 to 215,864
_01015
IAI47_01020: IAI47_01020 - acetolactate synthase 2 catalytic subunit, at 216,015 to 217,661
_01020
Position (kb) 215
216 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 214.769 kb on + strand, within IAI47_01010 at 214.769 kb on + strand, within IAI47_01010 at 214.802 kb on + strand, within IAI47_01010 at 214.803 kb on - strand, within IAI47_01010 at 214.803 kb on - strand, within IAI47_01010 at 214.803 kb on - strand, within IAI47_01010 at 214.803 kb on - strand, within IAI47_01010 at 214.803 kb on - strand, within IAI47_01010 at 214.904 kb on - strand, within IAI47_01010 at 214.904 kb on - strand, within IAI47_01010 at 214.931 kb on + strand, within IAI47_01010 at 214.948 kb on - strand, within IAI47_01010 at 214.948 kb on - strand, within IAI47_01010 at 214.948 kb on - strand, within IAI47_01010 at 215.047 kb on - strand, within IAI47_01010 at 215.057 kb on + strand, within IAI47_01010 at 215.057 kb on + strand, within IAI47_01010 at 215.057 kb on + strand, within IAI47_01010 at 215.079 kb on + strand, within IAI47_01010 at 215.080 kb on - strand, within IAI47_01010 at 215.080 kb on - strand, within IAI47_01010 at 215.125 kb on + strand, within IAI47_01010 at 215.125 kb on + strand, within IAI47_01010 at 215.125 kb on + strand, within IAI47_01010 at 215.125 kb on + strand, within IAI47_01010 at 215.125 kb on + strand, within IAI47_01010 at 215.125 kb on + strand, within IAI47_01010 at 215.125 kb on + strand, within IAI47_01010 at 215.125 kb on + strand, within IAI47_01010 at 215.125 kb on + strand, within IAI47_01010 at 215.126 kb on - strand, within IAI47_01010 at 215.126 kb on - strand, within IAI47_01010 at 215.126 kb on - strand, within IAI47_01010 at 215.126 kb on - strand, within IAI47_01010 at 215.138 kb on + strand, within IAI47_01010 at 215.183 kb on + strand, within IAI47_01010 at 215.184 kb on - strand, within IAI47_01010 at 215.184 kb on - strand, within IAI47_01010 at 215.187 kb on + strand, within IAI47_01010 at 215.187 kb on + strand, within IAI47_01010 at 215.187 kb on + strand, within IAI47_01010 at 215.187 kb on + strand, within IAI47_01010 at 215.187 kb on + strand, within IAI47_01010 at 215.187 kb on + strand, within IAI47_01010 at 215.187 kb on + strand, within IAI47_01010 at 215.187 kb on + strand, within IAI47_01010 at 215.187 kb on + strand, within IAI47_01010 at 215.187 kb on + strand, within IAI47_01010 at 215.187 kb on + strand, within IAI47_01010 at 215.188 kb on - strand, within IAI47_01010 at 215.188 kb on - strand, within IAI47_01010 at 215.188 kb on - strand, within IAI47_01010 at 215.188 kb on - strand, within IAI47_01010 at 215.188 kb on - strand, within IAI47_01010 at 215.188 kb on - strand, within IAI47_01010 at 215.188 kb on - strand, within IAI47_01010 at 215.188 kb on - strand, within IAI47_01010 at 215.188 kb on - strand, within IAI47_01010 at 215.188 kb on - strand, within IAI47_01010 at 215.188 kb on - strand, within IAI47_01010 at 215.188 kb on - strand, within IAI47_01010 at 215.188 kb on - strand, within IAI47_01010 at 215.188 kb on - strand, within IAI47_01010 at 215.188 kb on - strand, within IAI47_01010 at 215.188 kb on - strand, within IAI47_01010 at 215.188 kb on - strand, within IAI47_01010 at 215.188 kb on - strand, within IAI47_01010 at 215.188 kb on - strand, within IAI47_01010 at 215.215 kb on - strand, within IAI47_01010 at 215.215 kb on - strand, within IAI47_01010 at 215.276 kb on + strand, within IAI47_01010 at 215.277 kb on - strand, within IAI47_01010 at 215.340 kb on + strand at 215.340 kb on + strand at 215.363 kb on + strand at 215.363 kb on + strand at 215.363 kb on + strand at 215.394 kb on + strand at 215.394 kb on + strand at 215.394 kb on + strand at 215.394 kb on + strand at 215.394 kb on + strand at 215.394 kb on + strand at 215.394 kb on + strand at 215.395 kb on - strand at 215.395 kb on - strand at 215.395 kb on - strand at 215.395 kb on - strand at 215.400 kb on + strand at 215.401 kb on - strand at 215.401 kb on - strand at 215.427 kb on + strand at 215.435 kb on + strand at 215.435 kb on + strand at 215.436 kb on - strand at 215.436 kb on - strand at 215.436 kb on - strand at 215.436 kb on - strand at 215.588 kb on + strand at 215.588 kb on + strand at 215.589 kb on - strand at 215.589 kb on - strand at 215.596 kb on + strand at 215.596 kb on + strand at 215.597 kb on - strand at 215.608 kb on + strand at 215.608 kb on + strand at 215.608 kb on + strand at 215.608 kb on + strand at 215.608 kb on + strand at 215.608 kb on + strand at 215.609 kb on - strand at 215.612 kb on + strand at 215.613 kb on - strand at 215.613 kb on - strand at 215.613 kb on - strand at 215.613 kb on - strand at 215.613 kb on - strand at 215.623 kb on - strand at 215.623 kb on - strand at 215.623 kb on - strand at 215.635 kb on + strand at 215.635 kb on + strand at 215.636 kb on - strand at 215.636 kb on - strand at 215.636 kb on - strand at 215.638 kb on + strand at 215.638 kb on + strand at 215.649 kb on - strand at 215.649 kb on - strand at 215.649 kb on - strand at 215.704 kb on - strand at 215.714 kb on + strand at 215.715 kb on - strand at 215.715 kb on - strand at 215.734 kb on + strand at 215.735 kb on - strand at 215.735 kb on - strand at 215.735 kb on - strand at 215.735 kb on - strand at 215.747 kb on + strand at 215.747 kb on + strand at 215.748 kb on - strand at 215.779 kb on + strand, within IAI47_01015 at 215.779 kb on + strand, within IAI47_01015 at 215.779 kb on + strand, within IAI47_01015 at 215.779 kb on + strand, within IAI47_01015 at 215.779 kb on + strand, within IAI47_01015 at 215.780 kb on - strand, within IAI47_01015 at 215.780 kb on - strand, within IAI47_01015 at 215.790 kb on - strand, within IAI47_01015 at 215.790 kb on - strand, within IAI47_01015 at 215.819 kb on + strand, within IAI47_01015 at 215.819 kb on + strand, within IAI47_01015 at 215.820 kb on - strand, within IAI47_01015 at 215.822 kb on + strand, within IAI47_01015 at 215.823 kb on - strand, within IAI47_01015 at 215.823 kb on - strand, within IAI47_01015 at 215.823 kb on - strand, within IAI47_01015 at 215.827 kb on + strand, within IAI47_01015 at 215.827 kb on + strand, within IAI47_01015 at 215.935 kb on + strand at 215.935 kb on + strand at 215.971 kb on - strand at 215.975 kb on + strand at 215.975 kb on + strand at 215.975 kb on + strand at 215.976 kb on - strand at 215.976 kb on - strand at 216.034 kb on + strand at 216.034 kb on + strand at 216.034 kb on + strand at 216.034 kb on + strand at 216.034 kb on + strand at 216.035 kb on - strand at 216.035 kb on - strand at 216.062 kb on + strand at 216.062 kb on + strand at 216.062 kb on + strand at 216.063 kb on - strand at 216.063 kb on - strand at 216.063 kb on - strand at 216.063 kb on - strand at 216.065 kb on + strand at 216.065 kb on + strand at 216.066 kb on - strand at 216.066 kb on - strand at 216.070 kb on + strand at 216.070 kb on + strand at 216.070 kb on + strand at 216.070 kb on + strand at 216.071 kb on - strand at 216.071 kb on - strand at 216.071 kb on - strand at 216.071 kb on - strand at 216.105 kb on + strand at 216.105 kb on + strand at 216.105 kb on + strand at 216.105 kb on + strand at 216.106 kb on - strand at 216.106 kb on - strand at 216.106 kb on - strand at 216.106 kb on - strand at 216.117 kb on + strand at 216.117 kb on + strand at 216.117 kb on + strand at 216.117 kb on + strand at 216.117 kb on + strand at 216.118 kb on - strand at 216.118 kb on - strand at 216.118 kb on - strand at 216.118 kb on - strand at 216.118 kb on - strand at 216.118 kb on - strand at 216.118 kb on - strand at 216.139 kb on + strand at 216.139 kb on + strand at 216.139 kb on + strand at 216.139 kb on + strand at 216.139 kb on + strand at 216.139 kb on + strand at 216.139 kb on + strand at 216.139 kb on + strand at 216.139 kb on + strand at 216.139 kb on + strand at 216.139 kb on + strand at 216.139 kb on + strand at 216.139 kb on + strand at 216.139 kb on + strand at 216.139 kb on + strand at 216.140 kb on - strand at 216.140 kb on - strand at 216.140 kb on - strand at 216.140 kb on - strand at 216.140 kb on - strand at 216.140 kb on - strand at 216.140 kb on - strand at 216.140 kb on - strand at 216.140 kb on - strand at 216.140 kb on - strand at 216.141 kb on - strand at 216.183 kb on + strand, within IAI47_01020 at 216.183 kb on + strand, within IAI47_01020 at 216.216 kb on - strand, within IAI47_01020 at 216.216 kb on - strand, within IAI47_01020 at 216.377 kb on + strand, within IAI47_01020 at 216.377 kb on + strand, within IAI47_01020 at 216.378 kb on - strand, within IAI47_01020 at 216.378 kb on - strand, within IAI47_01020 at 216.378 kb on - strand, within IAI47_01020 at 216.378 kb on - strand, within IAI47_01020 at 216.378 kb on - strand, within IAI47_01020 at 216.422 kb on + strand, within IAI47_01020 at 216.423 kb on - strand, within IAI47_01020 at 216.538 kb on + strand, within IAI47_01020 at 216.618 kb on + strand, within IAI47_01020 at 216.618 kb on + strand, within IAI47_01020 at 216.618 kb on + strand, within IAI47_01020 at 216.618 kb on + strand, within IAI47_01020 at 216.618 kb on + strand, within IAI47_01020 at 216.619 kb on - strand, within IAI47_01020 at 216.619 kb on - strand, within IAI47_01020 at 216.619 kb on - strand, within IAI47_01020 at 216.619 kb on - strand, within IAI47_01020 at 216.715 kb on + strand, within IAI47_01020 at 216.715 kb on + strand, within IAI47_01020 at 216.715 kb on + strand, within IAI47_01020 at 216.732 kb on + strand, within IAI47_01020 at 216.732 kb on + strand, within IAI47_01020 at 216.732 kb on + strand, within IAI47_01020 at 216.732 kb on + strand, within IAI47_01020 at 216.732 kb on + strand, within IAI47_01020 at 216.732 kb on + strand, within IAI47_01020 at 216.732 kb on + strand, within IAI47_01020 at 216.733 kb on - strand, within IAI47_01020 at 216.733 kb on - strand, within IAI47_01020 at 216.744 kb on + strand, within IAI47_01020 at 216.744 kb on + strand, within IAI47_01020 at 216.745 kb on - strand, within IAI47_01020 at 216.745 kb on - strand, within IAI47_01020 at 216.745 kb on - strand, within IAI47_01020 at 216.745 kb on - strand, within IAI47_01020 at 216.793 kb on + strand, within IAI47_01020 at 216.793 kb on + strand, within IAI47_01020 at 216.860 kb on + strand, within IAI47_01020 at 216.860 kb on + strand, within IAI47_01020
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 214,769 + IAI47_01010 0.56 +0.2 214,769 + IAI47_01010 0.56 +0.4 214,802 + IAI47_01010 0.58 +0.1 214,803 - IAI47_01010 0.58 -0.5 214,803 - IAI47_01010 0.58 -0.4 214,803 - IAI47_01010 0.58 +0.6 214,803 - IAI47_01010 0.58 -0.5 214,803 - IAI47_01010 0.58 -0.3 214,904 - IAI47_01010 0.65 -0.7 214,904 - IAI47_01010 0.65 -1.9 214,931 + IAI47_01010 0.67 +0.4 214,948 - IAI47_01010 0.68 -2.3 214,948 - IAI47_01010 0.68 -2.5 214,948 - IAI47_01010 0.68 +1.4 215,047 - IAI47_01010 0.74 +1.7 215,057 + IAI47_01010 0.75 +0.0 215,057 + IAI47_01010 0.75 +2.8 215,057 + IAI47_01010 0.75 +0.2 215,079 + IAI47_01010 0.77 +2.9 215,080 - IAI47_01010 0.77 +0.9 215,080 - IAI47_01010 0.77 +4.0 215,125 + IAI47_01010 0.80 -0.9 215,125 + IAI47_01010 0.80 -1.1 215,125 + IAI47_01010 0.80 -0.7 215,125 + IAI47_01010 0.80 +1.7 215,125 + IAI47_01010 0.80 +1.2 215,125 + IAI47_01010 0.80 +0.0 215,125 + IAI47_01010 0.80 +0.7 215,125 + IAI47_01010 0.80 +0.7 215,125 + IAI47_01010 0.80 +0.8 215,126 - IAI47_01010 0.80 -0.6 215,126 - IAI47_01010 0.80 -0.6 215,126 - IAI47_01010 0.80 +0.7 215,126 - IAI47_01010 0.80 +0.2 215,138 + IAI47_01010 0.80 -0.5 215,183 + IAI47_01010 0.83 -1.6 215,184 - IAI47_01010 0.83 -0.6 215,184 - IAI47_01010 0.83 +1.8 215,187 + IAI47_01010 0.84 +1.7 215,187 + IAI47_01010 0.84 -0.9 215,187 + IAI47_01010 0.84 +1.1 215,187 + IAI47_01010 0.84 +1.0 215,187 + IAI47_01010 0.84 +0.3 215,187 + IAI47_01010 0.84 +0.8 215,187 + IAI47_01010 0.84 +1.7 215,187 + IAI47_01010 0.84 +0.4 215,187 + IAI47_01010 0.84 +1.1 215,187 + IAI47_01010 0.84 -0.5 215,187 + IAI47_01010 0.84 -0.2 215,188 - IAI47_01010 0.84 -2.1 215,188 - IAI47_01010 0.84 +0.6 215,188 - IAI47_01010 0.84 -2.0 215,188 - IAI47_01010 0.84 -0.5 215,188 - IAI47_01010 0.84 +0.8 215,188 - IAI47_01010 0.84 +2.9 215,188 - IAI47_01010 0.84 -0.7 215,188 - IAI47_01010 0.84 +1.4 215,188 - IAI47_01010 0.84 -1.7 215,188 - IAI47_01010 0.84 -1.4 215,188 - IAI47_01010 0.84 -0.7 215,188 - IAI47_01010 0.84 +1.3 215,188 - IAI47_01010 0.84 +0.4 215,188 - IAI47_01010 0.84 +1.0 215,188 - IAI47_01010 0.84 +0.1 215,188 - IAI47_01010 0.84 -0.9 215,188 - IAI47_01010 0.84 -1.2 215,188 - IAI47_01010 0.84 +0.3 215,188 - IAI47_01010 0.84 -0.2 215,215 - IAI47_01010 0.85 +0.6 215,215 - IAI47_01010 0.85 -0.6 215,276 + IAI47_01010 0.89 -0.2 215,277 - IAI47_01010 0.90 +1.2 215,340 + -0.3 215,340 + -0.1 215,363 + +0.1 215,363 + -1.4 215,363 + +1.8 215,394 + +0.6 215,394 + -0.6 215,394 + -0.4 215,394 + -1.5 215,394 + +1.1 215,394 + +0.1 215,394 + -0.8 215,395 - +0.3 215,395 - -1.0 215,395 - +0.2 215,395 - -0.7 215,400 + -0.2 215,401 - -0.6 215,401 - -0.1 215,427 + +0.1 215,435 + +1.0 215,435 + -1.0 215,436 - -0.1 215,436 - +0.0 215,436 - +0.1 215,436 - -1.9 215,588 + +1.8 215,588 + +0.5 215,589 - -1.0 215,589 - +0.3 215,596 + +1.2 215,596 + -0.1 215,597 - +0.2 215,608 + -0.1 215,608 + +0.1 215,608 + +0.7 215,608 + -0.7 215,608 + +0.2 215,608 + -0.8 215,609 - +0.4 215,612 + -2.0 215,613 - -0.3 215,613 - +0.7 215,613 - +0.5 215,613 - +0.4 215,613 - -0.2 215,623 - +2.6 215,623 - -1.4 215,623 - -1.1 215,635 + +0.8 215,635 + -0.6 215,636 - -1.0 215,636 - -0.5 215,636 - +0.1 215,638 + +0.1 215,638 + +0.6 215,649 - -0.5 215,649 - -0.8 215,649 - +0.6 215,704 - -0.1 215,714 + -0.5 215,715 - +1.1 215,715 - +1.3 215,734 + -2.8 215,735 - +1.1 215,735 - -1.0 215,735 - -1.0 215,735 - +0.9 215,747 + +1.5 215,747 + +1.1 215,748 - -0.1 215,779 + IAI47_01015 0.13 -2.3 215,779 + IAI47_01015 0.13 +0.0 215,779 + IAI47_01015 0.13 -0.2 215,779 + IAI47_01015 0.13 +1.0 215,779 + IAI47_01015 0.13 -1.0 215,780 - IAI47_01015 0.14 -0.5 215,780 - IAI47_01015 0.14 -0.9 215,790 - IAI47_01015 0.24 -0.1 215,790 - IAI47_01015 0.24 -1.3 215,819 + IAI47_01015 0.54 -1.2 215,819 + IAI47_01015 0.54 -1.0 215,820 - IAI47_01015 0.55 -1.5 215,822 + IAI47_01015 0.57 -1.5 215,823 - IAI47_01015 0.58 -0.1 215,823 - IAI47_01015 0.58 -0.2 215,823 - IAI47_01015 0.58 +1.8 215,827 + IAI47_01015 0.62 +0.1 215,827 + IAI47_01015 0.62 +0.9 215,935 + -0.3 215,935 + -1.5 215,971 - +0.1 215,975 + +1.1 215,975 + -0.7 215,975 + -2.5 215,976 - +0.7 215,976 - -0.2 216,034 + +0.6 216,034 + -0.1 216,034 + -1.1 216,034 + +0.5 216,034 + +0.6 216,035 - +0.5 216,035 - +1.1 216,062 + -0.0 216,062 + +1.1 216,062 + +1.2 216,063 - +0.6 216,063 - +2.0 216,063 - +0.2 216,063 - +0.2 216,065 + +0.6 216,065 + +1.3 216,066 - +0.2 216,066 - +1.3 216,070 + -0.9 216,070 + -1.7 216,070 + -0.2 216,070 + -0.7 216,071 - +1.2 216,071 - -1.5 216,071 - +0.8 216,071 - -2.3 216,105 + -0.1 216,105 + +1.5 216,105 + +1.6 216,105 + -0.2 216,106 - -0.8 216,106 - -0.7 216,106 - -0.5 216,106 - -0.4 216,117 + -1.1 216,117 + +0.9 216,117 + -0.9 216,117 + +1.7 216,117 + -1.2 216,118 - -2.1 216,118 - -1.1 216,118 - -1.1 216,118 - +2.0 216,118 - +0.6 216,118 - -0.9 216,118 - -1.3 216,139 + +0.1 216,139 + -0.5 216,139 + +0.3 216,139 + +2.4 216,139 + +0.7 216,139 + +0.9 216,139 + -0.6 216,139 + +0.6 216,139 + -0.2 216,139 + -0.1 216,139 + -0.3 216,139 + +2.3 216,139 + -1.3 216,139 + +1.4 216,139 + -0.2 216,140 - +0.6 216,140 - -0.2 216,140 - +0.1 216,140 - +0.5 216,140 - -0.2 216,140 - +0.6 216,140 - -0.5 216,140 - +0.8 216,140 - -0.5 216,140 - +0.0 216,141 - +2.9 216,183 + IAI47_01020 0.10 +0.6 216,183 + IAI47_01020 0.10 -2.8 216,216 - IAI47_01020 0.12 -1.1 216,216 - IAI47_01020 0.12 -1.8 216,377 + IAI47_01020 0.22 -0.2 216,377 + IAI47_01020 0.22 +2.6 216,378 - IAI47_01020 0.22 +0.9 216,378 - IAI47_01020 0.22 +1.7 216,378 - IAI47_01020 0.22 +1.5 216,378 - IAI47_01020 0.22 -0.7 216,378 - IAI47_01020 0.22 -0.1 216,422 + IAI47_01020 0.25 +0.3 216,423 - IAI47_01020 0.25 -0.0 216,538 + IAI47_01020 0.32 +0.2 216,618 + IAI47_01020 0.37 -0.5 216,618 + IAI47_01020 0.37 -0.4 216,618 + IAI47_01020 0.37 +0.4 216,618 + IAI47_01020 0.37 -0.1 216,618 + IAI47_01020 0.37 -0.4 216,619 - IAI47_01020 0.37 +1.0 216,619 - IAI47_01020 0.37 -0.9 216,619 - IAI47_01020 0.37 +0.3 216,619 - IAI47_01020 0.37 -0.7 216,715 + IAI47_01020 0.43 +0.9 216,715 + IAI47_01020 0.43 +0.9 216,715 + IAI47_01020 0.43 -0.8 216,732 + IAI47_01020 0.44 -0.2 216,732 + IAI47_01020 0.44 +0.7 216,732 + IAI47_01020 0.44 +0.6 216,732 + IAI47_01020 0.44 -0.6 216,732 + IAI47_01020 0.44 -1.2 216,732 + IAI47_01020 0.44 +0.6 216,732 + IAI47_01020 0.44 -1.1 216,733 - IAI47_01020 0.44 +0.1 216,733 - IAI47_01020 0.44 +2.4 216,744 + IAI47_01020 0.44 -0.1 216,744 + IAI47_01020 0.44 +0.6 216,745 - IAI47_01020 0.44 +1.2 216,745 - IAI47_01020 0.44 -3.0 216,745 - IAI47_01020 0.44 -0.4 216,745 - IAI47_01020 0.44 +0.1 216,793 + IAI47_01020 0.47 -0.3 216,793 + IAI47_01020 0.47 -1.3 216,860 + IAI47_01020 0.51 +0.7 216,860 + IAI47_01020 0.51 +0.4
Or see this region's nucleotide sequence