Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_00210 and IAI47_00215 are separated by 104 nucleotides IAI47_00215 and IAI47_00220 are separated by 17 nucleotides IAI47_00220 and IAI47_00225 are separated by 44 nucleotides
IAI47_00210: IAI47_00210 - MFS transporter, at 46,064 to 47,305
_00210
IAI47_00215: IAI47_00215 - phosphate signaling complex protein PhoU, at 47,410 to 48,144
_00215
IAI47_00220: IAI47_00220 - phosphate ABC transporter ATP-binding protein PstB, at 48,162 to 48,935
_00220
IAI47_00225: IAI47_00225 - phosphate ABC transporter permease PstA, at 48,980 to 49,870
_00225
Position (kb) 47
48
49 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 46.423 kb on - strand, within IAI47_00210 at 46.560 kb on + strand, within IAI47_00210 at 46.560 kb on + strand, within IAI47_00210 at 46.561 kb on - strand, within IAI47_00210 at 46.561 kb on - strand, within IAI47_00210 at 46.561 kb on - strand, within IAI47_00210 at 46.587 kb on + strand, within IAI47_00210 at 46.587 kb on + strand, within IAI47_00210 at 46.587 kb on + strand, within IAI47_00210 at 46.588 kb on - strand, within IAI47_00210 at 46.588 kb on - strand, within IAI47_00210 at 46.590 kb on + strand, within IAI47_00210 at 46.590 kb on + strand, within IAI47_00210 at 46.619 kb on - strand, within IAI47_00210 at 46.619 kb on - strand, within IAI47_00210 at 46.639 kb on + strand, within IAI47_00210 at 46.639 kb on + strand, within IAI47_00210 at 46.705 kb on + strand, within IAI47_00210 at 46.706 kb on - strand, within IAI47_00210 at 46.719 kb on + strand, within IAI47_00210 at 46.719 kb on + strand, within IAI47_00210 at 46.720 kb on - strand, within IAI47_00210 at 46.806 kb on - strand, within IAI47_00210 at 46.810 kb on + strand, within IAI47_00210 at 46.810 kb on + strand, within IAI47_00210 at 46.811 kb on - strand, within IAI47_00210 at 46.811 kb on - strand, within IAI47_00210 at 46.811 kb on - strand, within IAI47_00210 at 46.811 kb on - strand, within IAI47_00210 at 46.811 kb on - strand, within IAI47_00210 at 46.811 kb on - strand, within IAI47_00210 at 46.811 kb on - strand, within IAI47_00210 at 46.832 kb on - strand, within IAI47_00210 at 46.891 kb on + strand, within IAI47_00210 at 46.891 kb on + strand, within IAI47_00210 at 46.891 kb on + strand, within IAI47_00210 at 46.892 kb on - strand, within IAI47_00210 at 46.892 kb on - strand, within IAI47_00210 at 46.892 kb on - strand, within IAI47_00210 at 46.892 kb on - strand, within IAI47_00210 at 46.931 kb on - strand, within IAI47_00210 at 47.029 kb on + strand, within IAI47_00210 at 47.029 kb on + strand, within IAI47_00210 at 47.029 kb on + strand, within IAI47_00210 at 47.029 kb on + strand, within IAI47_00210 at 47.029 kb on + strand, within IAI47_00210 at 47.030 kb on - strand, within IAI47_00210 at 47.030 kb on - strand, within IAI47_00210 at 47.030 kb on - strand, within IAI47_00210 at 47.030 kb on - strand, within IAI47_00210 at 47.074 kb on - strand, within IAI47_00210 at 47.195 kb on - strand at 47.195 kb on - strand at 47.204 kb on + strand at 47.204 kb on + strand at 47.205 kb on - strand at 47.205 kb on - strand at 47.322 kb on + strand at 47.323 kb on - strand at 47.336 kb on + strand at 47.336 kb on + strand at 47.336 kb on + strand at 47.336 kb on + strand at 47.337 kb on - strand at 47.337 kb on - strand at 47.338 kb on + strand at 47.338 kb on + strand at 47.338 kb on + strand at 47.339 kb on - strand at 47.339 kb on - strand at 47.401 kb on + strand at 47.401 kb on + strand at 47.402 kb on - strand at 47.402 kb on - strand at 47.408 kb on + strand at 47.409 kb on - strand at 47.409 kb on - strand at 47.409 kb on - strand at 47.409 kb on - strand at 47.409 kb on - strand at 47.409 kb on - strand at 47.446 kb on + strand at 47.446 kb on + strand at 47.447 kb on - strand at 48.179 kb on + strand at 48.180 kb on - strand at 48.180 kb on - strand at 48.182 kb on + strand at 48.183 kb on - strand at 48.183 kb on - strand at 48.183 kb on - strand at 48.183 kb on - strand at 48.183 kb on - strand at 48.183 kb on - strand at 48.183 kb on - strand at 48.183 kb on - strand at 48.183 kb on - strand at 48.183 kb on - strand at 48.183 kb on - strand at 48.183 kb on - strand at 48.183 kb on - strand at 48.183 kb on - strand at 48.183 kb on - strand at 48.183 kb on - strand at 48.183 kb on - strand at 48.185 kb on - strand at 48.185 kb on - strand at 48.185 kb on - strand at 48.185 kb on - strand at 48.185 kb on - strand at 48.185 kb on - strand at 48.185 kb on - strand at 48.185 kb on - strand at 48.259 kb on + strand, within IAI47_00220 at 48.259 kb on + strand, within IAI47_00220 at 48.259 kb on + strand, within IAI47_00220 at 48.259 kb on + strand, within IAI47_00220 at 48.259 kb on + strand, within IAI47_00220 at 48.259 kb on + strand, within IAI47_00220 at 48.260 kb on - strand, within IAI47_00220 at 48.260 kb on - strand, within IAI47_00220 at 48.260 kb on - strand, within IAI47_00220 at 48.260 kb on - strand, within IAI47_00220 at 48.260 kb on - strand, within IAI47_00220 at 48.260 kb on - strand, within IAI47_00220 at 48.260 kb on - strand, within IAI47_00220 at 48.328 kb on + strand, within IAI47_00220 at 48.328 kb on + strand, within IAI47_00220 at 48.328 kb on + strand, within IAI47_00220 at 48.329 kb on - strand, within IAI47_00220 at 48.329 kb on - strand, within IAI47_00220 at 48.329 kb on - strand, within IAI47_00220 at 48.329 kb on - strand, within IAI47_00220 at 48.329 kb on - strand, within IAI47_00220 at 48.329 kb on - strand, within IAI47_00220 at 48.329 kb on - strand, within IAI47_00220 at 48.329 kb on - strand, within IAI47_00220 at 48.329 kb on - strand, within IAI47_00220 at 48.362 kb on + strand, within IAI47_00220 at 48.447 kb on - strand, within IAI47_00220 at 48.482 kb on - strand, within IAI47_00220 at 48.547 kb on - strand, within IAI47_00220 at 48.547 kb on - strand, within IAI47_00220 at 48.547 kb on - strand, within IAI47_00220 at 48.547 kb on - strand, within IAI47_00220 at 48.561 kb on - strand, within IAI47_00220 at 48.613 kb on + strand, within IAI47_00220 at 48.613 kb on + strand, within IAI47_00220 at 48.613 kb on + strand, within IAI47_00220 at 48.613 kb on + strand, within IAI47_00220 at 48.613 kb on + strand, within IAI47_00220 at 48.613 kb on + strand, within IAI47_00220 at 48.613 kb on + strand, within IAI47_00220 at 48.613 kb on + strand, within IAI47_00220 at 48.614 kb on - strand, within IAI47_00220 at 48.614 kb on - strand, within IAI47_00220 at 48.614 kb on - strand, within IAI47_00220 at 48.701 kb on + strand, within IAI47_00220 at 48.702 kb on - strand, within IAI47_00220 at 48.757 kb on + strand, within IAI47_00220 at 48.757 kb on + strand, within IAI47_00220 at 48.757 kb on + strand, within IAI47_00220 at 48.757 kb on + strand, within IAI47_00220 at 48.758 kb on - strand, within IAI47_00220 at 48.758 kb on - strand, within IAI47_00220 at 48.758 kb on - strand, within IAI47_00220 at 48.758 kb on - strand, within IAI47_00220 at 48.758 kb on - strand, within IAI47_00220 at 48.758 kb on - strand, within IAI47_00220 at 48.758 kb on - strand, within IAI47_00220 at 48.764 kb on + strand, within IAI47_00220 at 48.765 kb on - strand, within IAI47_00220 at 48.765 kb on - strand, within IAI47_00220 at 48.773 kb on + strand, within IAI47_00220 at 48.773 kb on + strand, within IAI47_00220 at 48.773 kb on + strand, within IAI47_00220 at 48.774 kb on - strand, within IAI47_00220 at 48.774 kb on - strand, within IAI47_00220 at 48.774 kb on - strand, within IAI47_00220 at 48.791 kb on + strand, within IAI47_00220 at 48.792 kb on - strand, within IAI47_00220 at 48.792 kb on - strand, within IAI47_00220 at 48.792 kb on - strand, within IAI47_00220 at 48.792 kb on - strand, within IAI47_00220 at 48.792 kb on - strand, within IAI47_00220 at 48.792 kb on - strand, within IAI47_00220 at 48.792 kb on - strand, within IAI47_00220 at 48.792 kb on - strand, within IAI47_00220 at 48.833 kb on + strand, within IAI47_00220 at 48.833 kb on + strand, within IAI47_00220 at 48.833 kb on + strand, within IAI47_00220 at 48.834 kb on - strand, within IAI47_00220 at 48.880 kb on + strand at 48.880 kb on + strand at 48.880 kb on + strand at 48.880 kb on + strand at 48.881 kb on - strand at 48.881 kb on - strand at 48.881 kb on - strand at 48.881 kb on - strand at 48.893 kb on + strand at 48.894 kb on - strand at 48.908 kb on + strand at 48.909 kb on - strand at 48.929 kb on + strand at 48.935 kb on + strand at 48.935 kb on + strand at 48.935 kb on + strand at 48.935 kb on + strand at 48.935 kb on + strand at 48.935 kb on + strand at 48.936 kb on - strand at 48.936 kb on - strand at 48.936 kb on - strand at 48.936 kb on - strand at 48.936 kb on - strand at 48.936 kb on - strand at 48.949 kb on + strand at 48.949 kb on + strand at 48.949 kb on + strand at 48.949 kb on + strand at 48.949 kb on + strand at 48.949 kb on + strand at 48.950 kb on - strand at 48.950 kb on - strand at 48.950 kb on - strand at 48.951 kb on + strand at 48.951 kb on + strand at 48.951 kb on + strand at 48.951 kb on + strand at 48.951 kb on + strand at 48.952 kb on - strand at 48.981 kb on + strand at 48.991 kb on + strand at 48.991 kb on + strand at 48.992 kb on - strand at 48.992 kb on - strand at 48.992 kb on - strand at 49.045 kb on + strand at 49.108 kb on + strand, within IAI47_00225 at 49.108 kb on + strand, within IAI47_00225
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 46,423 - IAI47_00210 0.29 +0.1 46,560 + IAI47_00210 0.40 +0.1 46,560 + IAI47_00210 0.40 -1.3 46,561 - IAI47_00210 0.40 -1.7 46,561 - IAI47_00210 0.40 -1.4 46,561 - IAI47_00210 0.40 -0.6 46,587 + IAI47_00210 0.42 -1.6 46,587 + IAI47_00210 0.42 -2.0 46,587 + IAI47_00210 0.42 -0.1 46,588 - IAI47_00210 0.42 +0.9 46,588 - IAI47_00210 0.42 +0.6 46,590 + IAI47_00210 0.42 -0.5 46,590 + IAI47_00210 0.42 +1.7 46,619 - IAI47_00210 0.45 +0.2 46,619 - IAI47_00210 0.45 +1.7 46,639 + IAI47_00210 0.46 -0.1 46,639 + IAI47_00210 0.46 -0.9 46,705 + IAI47_00210 0.52 -0.9 46,706 - IAI47_00210 0.52 -3.0 46,719 + IAI47_00210 0.53 -0.7 46,719 + IAI47_00210 0.53 +1.0 46,720 - IAI47_00210 0.53 -2.4 46,806 - IAI47_00210 0.60 -2.1 46,810 + IAI47_00210 0.60 -2.5 46,810 + IAI47_00210 0.60 +0.2 46,811 - IAI47_00210 0.60 +2.3 46,811 - IAI47_00210 0.60 -1.5 46,811 - IAI47_00210 0.60 +0.4 46,811 - IAI47_00210 0.60 -1.4 46,811 - IAI47_00210 0.60 +0.3 46,811 - IAI47_00210 0.60 +1.5 46,811 - IAI47_00210 0.60 -1.3 46,832 - IAI47_00210 0.62 -0.2 46,891 + IAI47_00210 0.67 +0.3 46,891 + IAI47_00210 0.67 -0.4 46,891 + IAI47_00210 0.67 +0.2 46,892 - IAI47_00210 0.67 -0.2 46,892 - IAI47_00210 0.67 -0.4 46,892 - IAI47_00210 0.67 +0.5 46,892 - IAI47_00210 0.67 +0.4 46,931 - IAI47_00210 0.70 -1.6 47,029 + IAI47_00210 0.78 -0.8 47,029 + IAI47_00210 0.78 +0.2 47,029 + IAI47_00210 0.78 -0.4 47,029 + IAI47_00210 0.78 +0.0 47,029 + IAI47_00210 0.78 +0.2 47,030 - IAI47_00210 0.78 -0.1 47,030 - IAI47_00210 0.78 +0.5 47,030 - IAI47_00210 0.78 +0.8 47,030 - IAI47_00210 0.78 +1.3 47,074 - IAI47_00210 0.81 -0.7 47,195 - +0.7 47,195 - -1.0 47,204 + -0.2 47,204 + +0.2 47,205 - +0.5 47,205 - -0.3 47,322 + -0.4 47,323 - -0.6 47,336 + -0.4 47,336 + -1.7 47,336 + -1.7 47,336 + +0.4 47,337 - -1.5 47,337 - -0.6 47,338 + -0.3 47,338 + +1.5 47,338 + -0.7 47,339 - +0.9 47,339 - +0.1 47,401 + -1.1 47,401 + +0.1 47,402 - -0.7 47,402 - +0.5 47,408 + +0.1 47,409 - -2.4 47,409 - -0.6 47,409 - -0.7 47,409 - -1.3 47,409 - +0.1 47,409 - +0.4 47,446 + +1.4 47,446 + +1.7 47,447 - -1.7 48,179 + +1.7 48,180 - +0.8 48,180 - +0.9 48,182 + +0.0 48,183 - -0.3 48,183 - +1.5 48,183 - -1.7 48,183 - -0.1 48,183 - -1.4 48,183 - -0.6 48,183 - -0.7 48,183 - -0.1 48,183 - +1.7 48,183 - +1.3 48,183 - +1.8 48,183 - -0.2 48,183 - -1.1 48,183 - -0.7 48,183 - -0.4 48,183 - +0.5 48,183 - +0.9 48,185 - -0.6 48,185 - -0.6 48,185 - -0.2 48,185 - -1.0 48,185 - -1.1 48,185 - +0.1 48,185 - +1.2 48,185 - +0.1 48,259 + IAI47_00220 0.13 -0.3 48,259 + IAI47_00220 0.13 -0.5 48,259 + IAI47_00220 0.13 -2.5 48,259 + IAI47_00220 0.13 -0.1 48,259 + IAI47_00220 0.13 +0.7 48,259 + IAI47_00220 0.13 -0.1 48,260 - IAI47_00220 0.13 +1.3 48,260 - IAI47_00220 0.13 -0.1 48,260 - IAI47_00220 0.13 -1.6 48,260 - IAI47_00220 0.13 +0.6 48,260 - IAI47_00220 0.13 -0.3 48,260 - IAI47_00220 0.13 -0.2 48,260 - IAI47_00220 0.13 -2.1 48,328 + IAI47_00220 0.21 +0.7 48,328 + IAI47_00220 0.21 +0.5 48,328 + IAI47_00220 0.21 -0.0 48,329 - IAI47_00220 0.22 -0.9 48,329 - IAI47_00220 0.22 -1.0 48,329 - IAI47_00220 0.22 -0.4 48,329 - IAI47_00220 0.22 -0.7 48,329 - IAI47_00220 0.22 -0.3 48,329 - IAI47_00220 0.22 +0.2 48,329 - IAI47_00220 0.22 +1.1 48,329 - IAI47_00220 0.22 -0.8 48,329 - IAI47_00220 0.22 -0.0 48,362 + IAI47_00220 0.26 +1.1 48,447 - IAI47_00220 0.37 -2.3 48,482 - IAI47_00220 0.41 -1.8 48,547 - IAI47_00220 0.50 -0.3 48,547 - IAI47_00220 0.50 -0.7 48,547 - IAI47_00220 0.50 -1.0 48,547 - IAI47_00220 0.50 -2.1 48,561 - IAI47_00220 0.52 -0.2 48,613 + IAI47_00220 0.58 +1.6 48,613 + IAI47_00220 0.58 +0.1 48,613 + IAI47_00220 0.58 +0.4 48,613 + IAI47_00220 0.58 -2.4 48,613 + IAI47_00220 0.58 +0.2 48,613 + IAI47_00220 0.58 -1.8 48,613 + IAI47_00220 0.58 -2.5 48,613 + IAI47_00220 0.58 +0.1 48,614 - IAI47_00220 0.58 -0.7 48,614 - IAI47_00220 0.58 -0.6 48,614 - IAI47_00220 0.58 +0.2 48,701 + IAI47_00220 0.70 +0.4 48,702 - IAI47_00220 0.70 -0.2 48,757 + IAI47_00220 0.77 -0.7 48,757 + IAI47_00220 0.77 +1.0 48,757 + IAI47_00220 0.77 +0.1 48,757 + IAI47_00220 0.77 -0.6 48,758 - IAI47_00220 0.77 +1.1 48,758 - IAI47_00220 0.77 +1.1 48,758 - IAI47_00220 0.77 -0.3 48,758 - IAI47_00220 0.77 +1.2 48,758 - IAI47_00220 0.77 -0.6 48,758 - IAI47_00220 0.77 -0.0 48,758 - IAI47_00220 0.77 -1.3 48,764 + IAI47_00220 0.78 +0.6 48,765 - IAI47_00220 0.78 -0.8 48,765 - IAI47_00220 0.78 +1.4 48,773 + IAI47_00220 0.79 +1.4 48,773 + IAI47_00220 0.79 -0.7 48,773 + IAI47_00220 0.79 +0.2 48,774 - IAI47_00220 0.79 +1.0 48,774 - IAI47_00220 0.79 +0.1 48,774 - IAI47_00220 0.79 -2.3 48,791 + IAI47_00220 0.81 -0.4 48,792 - IAI47_00220 0.81 -1.1 48,792 - IAI47_00220 0.81 -2.4 48,792 - IAI47_00220 0.81 -1.7 48,792 - IAI47_00220 0.81 -1.4 48,792 - IAI47_00220 0.81 -0.3 48,792 - IAI47_00220 0.81 -0.2 48,792 - IAI47_00220 0.81 -2.6 48,792 - IAI47_00220 0.81 +0.1 48,833 + IAI47_00220 0.87 -1.0 48,833 + IAI47_00220 0.87 +0.2 48,833 + IAI47_00220 0.87 +0.5 48,834 - IAI47_00220 0.87 -0.8 48,880 + -0.6 48,880 + +0.0 48,880 + -1.5 48,880 + +0.5 48,881 - -0.2 48,881 - +0.7 48,881 - -2.6 48,881 - -1.9 48,893 + +0.4 48,894 - -0.5 48,908 + -1.3 48,909 - -0.2 48,929 + -0.6 48,935 + +0.8 48,935 + -2.1 48,935 + -0.3 48,935 + +0.6 48,935 + -1.5 48,935 + +0.0 48,936 - -0.2 48,936 - -0.9 48,936 - +0.0 48,936 - +1.4 48,936 - -0.8 48,936 - +0.6 48,949 + -1.6 48,949 + +1.5 48,949 + -0.1 48,949 + -2.0 48,949 + -0.9 48,949 + -1.0 48,950 - -0.5 48,950 - -0.7 48,950 - +1.5 48,951 + +0.6 48,951 + -1.3 48,951 + +0.5 48,951 + +0.4 48,951 + +1.8 48,952 - -0.5 48,981 + +1.5 48,991 + +0.6 48,991 + -0.6 48,992 - -2.3 48,992 - -0.0 48,992 - +0.7 49,045 + -0.4 49,108 + IAI47_00225 0.14 +1.3 49,108 + IAI47_00225 0.14 -0.6
Or see this region's nucleotide sequence