Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20330 and IAI47_20335 are separated by 68 nucleotides IAI47_20335 and IAI47_20340 are separated by 26 nucleotides
IAI47_20330: IAI47_20330 - LacI family DNA-binding transcriptional regulator, at 304,391 to 305,371
_20330
IAI47_20335: IAI47_20335 - sugar phosphate isomerase/epimerase, at 305,440 to 306,501
_20335
IAI47_20340: IAI47_20340 - MFS transporter, at 306,528 to 307,757
_20340
Position (kb) 305
306
307 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 304.643 kb on - strand, within IAI47_20330 at 304.708 kb on + strand, within IAI47_20330 at 304.737 kb on + strand, within IAI47_20330 at 304.738 kb on - strand, within IAI47_20330 at 304.785 kb on + strand, within IAI47_20330 at 304.809 kb on + strand, within IAI47_20330 at 304.810 kb on - strand, within IAI47_20330 at 304.810 kb on - strand, within IAI47_20330 at 304.815 kb on + strand, within IAI47_20330 at 304.816 kb on - strand, within IAI47_20330 at 304.869 kb on + strand, within IAI47_20330 at 304.869 kb on + strand, within IAI47_20330 at 304.869 kb on + strand, within IAI47_20330 at 304.870 kb on - strand, within IAI47_20330 at 304.930 kb on + strand, within IAI47_20330 at 304.930 kb on + strand, within IAI47_20330 at 304.971 kb on - strand, within IAI47_20330 at 305.089 kb on + strand, within IAI47_20330 at 305.089 kb on + strand, within IAI47_20330 at 305.089 kb on + strand, within IAI47_20330 at 305.089 kb on + strand, within IAI47_20330 at 305.090 kb on - strand, within IAI47_20330 at 305.090 kb on - strand, within IAI47_20330 at 305.090 kb on - strand, within IAI47_20330 at 305.135 kb on - strand, within IAI47_20330 at 305.135 kb on - strand, within IAI47_20330 at 305.170 kb on + strand, within IAI47_20330 at 305.171 kb on - strand, within IAI47_20330 at 305.209 kb on + strand, within IAI47_20330 at 305.242 kb on - strand, within IAI47_20330 at 305.242 kb on - strand, within IAI47_20330 at 305.242 kb on - strand, within IAI47_20330 at 305.242 kb on - strand, within IAI47_20330 at 305.305 kb on + strand at 305.306 kb on - strand at 305.306 kb on - strand at 305.364 kb on - strand at 305.364 kb on - strand at 305.365 kb on + strand at 305.365 kb on + strand at 305.365 kb on + strand at 305.365 kb on + strand at 305.365 kb on + strand at 305.366 kb on - strand at 305.366 kb on - strand at 305.366 kb on - strand at 305.366 kb on - strand at 305.366 kb on - strand at 305.371 kb on + strand at 305.371 kb on + strand at 305.372 kb on - strand at 305.372 kb on - strand at 305.372 kb on - strand at 305.372 kb on - strand at 305.372 kb on - strand at 305.410 kb on + strand at 305.410 kb on + strand at 305.410 kb on + strand at 305.410 kb on + strand at 305.410 kb on + strand at 305.410 kb on + strand at 305.410 kb on + strand at 305.410 kb on + strand at 305.411 kb on - strand at 305.411 kb on - strand at 305.411 kb on - strand at 305.411 kb on - strand at 305.411 kb on - strand at 305.411 kb on - strand at 305.411 kb on - strand at 305.411 kb on - strand at 305.411 kb on - strand at 305.473 kb on + strand at 305.474 kb on - strand at 305.474 kb on - strand at 305.474 kb on - strand at 305.535 kb on + strand at 305.535 kb on + strand at 305.536 kb on - strand at 305.608 kb on + strand, within IAI47_20335 at 305.609 kb on - strand, within IAI47_20335 at 305.625 kb on - strand, within IAI47_20335 at 305.626 kb on - strand, within IAI47_20335 at 305.630 kb on + strand, within IAI47_20335 at 305.630 kb on + strand, within IAI47_20335 at 305.631 kb on - strand, within IAI47_20335 at 305.631 kb on - strand, within IAI47_20335 at 305.631 kb on - strand, within IAI47_20335 at 305.652 kb on + strand, within IAI47_20335 at 305.653 kb on - strand, within IAI47_20335 at 305.720 kb on + strand, within IAI47_20335 at 305.757 kb on - strand, within IAI47_20335 at 305.757 kb on - strand, within IAI47_20335 at 305.798 kb on + strand, within IAI47_20335 at 305.798 kb on + strand, within IAI47_20335 at 305.799 kb on - strand, within IAI47_20335 at 305.799 kb on - strand, within IAI47_20335 at 305.819 kb on + strand, within IAI47_20335 at 305.819 kb on + strand, within IAI47_20335 at 305.819 kb on + strand, within IAI47_20335 at 305.819 kb on + strand, within IAI47_20335 at 305.819 kb on + strand, within IAI47_20335 at 305.862 kb on + strand, within IAI47_20335 at 305.862 kb on + strand, within IAI47_20335 at 305.883 kb on + strand, within IAI47_20335 at 305.948 kb on + strand, within IAI47_20335 at 305.948 kb on + strand, within IAI47_20335 at 306.060 kb on - strand, within IAI47_20335 at 306.187 kb on + strand, within IAI47_20335 at 306.187 kb on + strand, within IAI47_20335 at 306.187 kb on + strand, within IAI47_20335 at 306.188 kb on - strand, within IAI47_20335 at 306.188 kb on - strand, within IAI47_20335 at 306.208 kb on - strand, within IAI47_20335 at 306.224 kb on + strand, within IAI47_20335 at 306.241 kb on + strand, within IAI47_20335 at 306.265 kb on - strand, within IAI47_20335 at 306.323 kb on + strand, within IAI47_20335 at 306.323 kb on + strand, within IAI47_20335 at 306.323 kb on + strand, within IAI47_20335 at 306.323 kb on + strand, within IAI47_20335 at 306.324 kb on - strand, within IAI47_20335 at 306.324 kb on - strand, within IAI47_20335 at 306.324 kb on - strand, within IAI47_20335 at 306.324 kb on - strand, within IAI47_20335 at 306.324 kb on - strand, within IAI47_20335 at 306.349 kb on - strand, within IAI47_20335 at 306.360 kb on + strand, within IAI47_20335 at 306.396 kb on - strand at 306.438 kb on + strand at 306.489 kb on + strand at 306.490 kb on - strand at 306.505 kb on + strand at 306.505 kb on + strand at 306.505 kb on + strand at 306.506 kb on - strand at 306.506 kb on - strand at 306.506 kb on - strand at 306.506 kb on - strand at 306.529 kb on + strand at 306.530 kb on - strand at 306.530 kb on - strand at 306.530 kb on - strand at 306.530 kb on - strand at 306.538 kb on + strand at 306.538 kb on + strand at 306.539 kb on - strand at 306.539 kb on - strand at 306.560 kb on + strand at 306.560 kb on + strand at 306.560 kb on + strand at 306.560 kb on + strand at 306.561 kb on - strand at 306.649 kb on + strand at 306.649 kb on + strand at 306.649 kb on + strand at 306.650 kb on - strand at 306.694 kb on + strand, within IAI47_20340 at 306.695 kb on - strand, within IAI47_20340 at 306.695 kb on - strand, within IAI47_20340 at 306.695 kb on - strand, within IAI47_20340 at 306.695 kb on - strand, within IAI47_20340 at 306.695 kb on - strand, within IAI47_20340 at 306.695 kb on - strand, within IAI47_20340 at 306.760 kb on + strand, within IAI47_20340 at 306.761 kb on - strand, within IAI47_20340 at 306.761 kb on - strand, within IAI47_20340 at 306.765 kb on - strand, within IAI47_20340 at 306.765 kb on - strand, within IAI47_20340 at 306.765 kb on - strand, within IAI47_20340 at 306.820 kb on + strand, within IAI47_20340 at 306.868 kb on + strand, within IAI47_20340 at 307.039 kb on + strand, within IAI47_20340 at 307.039 kb on + strand, within IAI47_20340 at 307.040 kb on - strand, within IAI47_20340 at 307.040 kb on - strand, within IAI47_20340 at 307.042 kb on + strand, within IAI47_20340 at 307.042 kb on + strand, within IAI47_20340 at 307.042 kb on + strand, within IAI47_20340 at 307.042 kb on + strand, within IAI47_20340 at 307.042 kb on + strand, within IAI47_20340 at 307.043 kb on - strand, within IAI47_20340 at 307.043 kb on - strand, within IAI47_20340 at 307.043 kb on - strand, within IAI47_20340 at 307.043 kb on - strand, within IAI47_20340 at 307.043 kb on - strand, within IAI47_20340 at 307.043 kb on - strand, within IAI47_20340 at 307.178 kb on + strand, within IAI47_20340 at 307.212 kb on + strand, within IAI47_20340 at 307.367 kb on + strand, within IAI47_20340 at 307.367 kb on + strand, within IAI47_20340 at 307.368 kb on - strand, within IAI47_20340 at 307.368 kb on - strand, within IAI47_20340 at 307.368 kb on - strand, within IAI47_20340 at 307.368 kb on - strand, within IAI47_20340 at 307.403 kb on + strand, within IAI47_20340 at 307.403 kb on + strand, within IAI47_20340 at 307.403 kb on + strand, within IAI47_20340 at 307.403 kb on + strand, within IAI47_20340 at 307.404 kb on - strand, within IAI47_20340 at 307.404 kb on - strand, within IAI47_20340 at 307.404 kb on - strand, within IAI47_20340 at 307.404 kb on - strand, within IAI47_20340 at 307.404 kb on - strand, within IAI47_20340 at 307.406 kb on - strand, within IAI47_20340 at 307.413 kb on + strand, within IAI47_20340 at 307.413 kb on + strand, within IAI47_20340 at 307.413 kb on + strand, within IAI47_20340 at 307.414 kb on - strand, within IAI47_20340 at 307.414 kb on - strand, within IAI47_20340
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 304,643 - IAI47_20330 0.26 +0.5 304,708 + IAI47_20330 0.32 +1.5 304,737 + IAI47_20330 0.35 +1.6 304,738 - IAI47_20330 0.35 +1.5 304,785 + IAI47_20330 0.40 -0.1 304,809 + IAI47_20330 0.43 -1.2 304,810 - IAI47_20330 0.43 +0.9 304,810 - IAI47_20330 0.43 +1.2 304,815 + IAI47_20330 0.43 -0.8 304,816 - IAI47_20330 0.43 -0.8 304,869 + IAI47_20330 0.49 -1.3 304,869 + IAI47_20330 0.49 +0.9 304,869 + IAI47_20330 0.49 +0.4 304,870 - IAI47_20330 0.49 -1.4 304,930 + IAI47_20330 0.55 -1.5 304,930 + IAI47_20330 0.55 +2.9 304,971 - IAI47_20330 0.59 +0.8 305,089 + IAI47_20330 0.71 +1.5 305,089 + IAI47_20330 0.71 -0.4 305,089 + IAI47_20330 0.71 +0.6 305,089 + IAI47_20330 0.71 +2.4 305,090 - IAI47_20330 0.71 +0.9 305,090 - IAI47_20330 0.71 +2.4 305,090 - IAI47_20330 0.71 +0.4 305,135 - IAI47_20330 0.76 +0.5 305,135 - IAI47_20330 0.76 -2.0 305,170 + IAI47_20330 0.79 +1.0 305,171 - IAI47_20330 0.80 +2.6 305,209 + IAI47_20330 0.83 +0.0 305,242 - IAI47_20330 0.87 -0.7 305,242 - IAI47_20330 0.87 +3.9 305,242 - IAI47_20330 0.87 -1.8 305,242 - IAI47_20330 0.87 -0.4 305,305 + +0.3 305,306 - +0.4 305,306 - -2.6 305,364 - +1.0 305,364 - -0.6 305,365 + +3.4 305,365 + -1.3 305,365 + -0.4 305,365 + +1.4 305,365 + +1.6 305,366 - +0.1 305,366 - +0.3 305,366 - -1.2 305,366 - -0.4 305,366 - +0.6 305,371 + +1.1 305,371 + +1.2 305,372 - +0.4 305,372 - -0.6 305,372 - +1.3 305,372 - -0.6 305,372 - +2.0 305,410 + -1.9 305,410 + -0.4 305,410 + -0.9 305,410 + -0.6 305,410 + -0.4 305,410 + +0.4 305,410 + +1.0 305,410 + -2.0 305,411 - +1.6 305,411 - +1.0 305,411 - +0.7 305,411 - -0.0 305,411 - +0.4 305,411 - +0.5 305,411 - -1.4 305,411 - +0.0 305,411 - -0.3 305,473 + -2.3 305,474 - +2.8 305,474 - -0.2 305,474 - -0.1 305,535 + -1.0 305,535 + +0.9 305,536 - -0.8 305,608 + IAI47_20335 0.16 +0.1 305,609 - IAI47_20335 0.16 -0.9 305,625 - IAI47_20335 0.17 -0.4 305,626 - IAI47_20335 0.18 -2.5 305,630 + IAI47_20335 0.18 -0.7 305,630 + IAI47_20335 0.18 +0.1 305,631 - IAI47_20335 0.18 -0.3 305,631 - IAI47_20335 0.18 -0.1 305,631 - IAI47_20335 0.18 -1.1 305,652 + IAI47_20335 0.20 +0.6 305,653 - IAI47_20335 0.20 +0.4 305,720 + IAI47_20335 0.26 +1.3 305,757 - IAI47_20335 0.30 +1.5 305,757 - IAI47_20335 0.30 +0.3 305,798 + IAI47_20335 0.34 +1.2 305,798 + IAI47_20335 0.34 -2.3 305,799 - IAI47_20335 0.34 -0.3 305,799 - IAI47_20335 0.34 +0.3 305,819 + IAI47_20335 0.36 +0.1 305,819 + IAI47_20335 0.36 -2.1 305,819 + IAI47_20335 0.36 -0.8 305,819 + IAI47_20335 0.36 -1.0 305,819 + IAI47_20335 0.36 +4.6 305,862 + IAI47_20335 0.40 -0.9 305,862 + IAI47_20335 0.40 -0.6 305,883 + IAI47_20335 0.42 -0.3 305,948 + IAI47_20335 0.48 +2.2 305,948 + IAI47_20335 0.48 -2.2 306,060 - IAI47_20335 0.58 +1.1 306,187 + IAI47_20335 0.70 -1.2 306,187 + IAI47_20335 0.70 -0.1 306,187 + IAI47_20335 0.70 -0.8 306,188 - IAI47_20335 0.70 +1.0 306,188 - IAI47_20335 0.70 -1.2 306,208 - IAI47_20335 0.72 +0.1 306,224 + IAI47_20335 0.74 -0.8 306,241 + IAI47_20335 0.75 +1.0 306,265 - IAI47_20335 0.78 +0.7 306,323 + IAI47_20335 0.83 -0.4 306,323 + IAI47_20335 0.83 -0.7 306,323 + IAI47_20335 0.83 -0.2 306,323 + IAI47_20335 0.83 -0.2 306,324 - IAI47_20335 0.83 -0.4 306,324 - IAI47_20335 0.83 -0.3 306,324 - IAI47_20335 0.83 +0.1 306,324 - IAI47_20335 0.83 -0.9 306,324 - IAI47_20335 0.83 -0.8 306,349 - IAI47_20335 0.86 +0.1 306,360 + IAI47_20335 0.87 +1.4 306,396 - +0.6 306,438 + -0.6 306,489 + +1.4 306,490 - -0.6 306,505 + -2.6 306,505 + +0.1 306,505 + -0.3 306,506 - -1.1 306,506 - +1.4 306,506 - -0.9 306,506 - +0.5 306,529 + +2.2 306,530 - +0.4 306,530 - -1.3 306,530 - -1.3 306,530 - -1.5 306,538 + -0.2 306,538 + -1.5 306,539 - +0.9 306,539 - -0.9 306,560 + +0.4 306,560 + -0.9 306,560 + +0.2 306,560 + -1.7 306,561 - +0.6 306,649 + +0.4 306,649 + +1.8 306,649 + -1.5 306,650 - -0.1 306,694 + IAI47_20340 0.13 +0.3 306,695 - IAI47_20340 0.14 +1.5 306,695 - IAI47_20340 0.14 -1.9 306,695 - IAI47_20340 0.14 +2.2 306,695 - IAI47_20340 0.14 +1.2 306,695 - IAI47_20340 0.14 +1.9 306,695 - IAI47_20340 0.14 -1.5 306,760 + IAI47_20340 0.19 +0.5 306,761 - IAI47_20340 0.19 -1.0 306,761 - IAI47_20340 0.19 -0.5 306,765 - IAI47_20340 0.19 +0.4 306,765 - IAI47_20340 0.19 +0.5 306,765 - IAI47_20340 0.19 -1.8 306,820 + IAI47_20340 0.24 +0.9 306,868 + IAI47_20340 0.28 -0.6 307,039 + IAI47_20340 0.42 +0.9 307,039 + IAI47_20340 0.42 +0.4 307,040 - IAI47_20340 0.42 -2.0 307,040 - IAI47_20340 0.42 -1.7 307,042 + IAI47_20340 0.42 -0.7 307,042 + IAI47_20340 0.42 -0.6 307,042 + IAI47_20340 0.42 +0.0 307,042 + IAI47_20340 0.42 -1.5 307,042 + IAI47_20340 0.42 +1.2 307,043 - IAI47_20340 0.42 -1.9 307,043 - IAI47_20340 0.42 +0.4 307,043 - IAI47_20340 0.42 +0.2 307,043 - IAI47_20340 0.42 +1.0 307,043 - IAI47_20340 0.42 +0.3 307,043 - IAI47_20340 0.42 -1.9 307,178 + IAI47_20340 0.53 -0.3 307,212 + IAI47_20340 0.56 -1.5 307,367 + IAI47_20340 0.68 +0.6 307,367 + IAI47_20340 0.68 +0.6 307,368 - IAI47_20340 0.68 -1.7 307,368 - IAI47_20340 0.68 +0.9 307,368 - IAI47_20340 0.68 -0.5 307,368 - IAI47_20340 0.68 +2.0 307,403 + IAI47_20340 0.71 +1.2 307,403 + IAI47_20340 0.71 -0.9 307,403 + IAI47_20340 0.71 -0.9 307,403 + IAI47_20340 0.71 -1.5 307,404 - IAI47_20340 0.71 -0.1 307,404 - IAI47_20340 0.71 -1.7 307,404 - IAI47_20340 0.71 -1.0 307,404 - IAI47_20340 0.71 -2.4 307,404 - IAI47_20340 0.71 -0.5 307,406 - IAI47_20340 0.71 -0.9 307,413 + IAI47_20340 0.72 -0.4 307,413 + IAI47_20340 0.72 -0.2 307,413 + IAI47_20340 0.72 +0.4 307,414 - IAI47_20340 0.72 +0.8 307,414 - IAI47_20340 0.72 +1.2
Or see this region's nucleotide sequence