Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_14795 and IAI47_14800 are separated by 25 nucleotides IAI47_14800 and IAI47_14805 overlap by 1 nucleotides IAI47_14805 and IAI47_14810 are separated by 77 nucleotides
IAI47_14795: IAI47_14795 - protein translocase subunit SecD, at 3,118,888 to 3,120,735
_14795
IAI47_14800: IAI47_14800 - preprotein translocase subunit YajC, at 3,120,761 to 3,121,093
_14800
IAI47_14805: IAI47_14805 - tRNA guanosine(34) transglycosylase Tgt, at 3,121,093 to 3,122,241
_14805
IAI47_14810: IAI47_14810 - tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA, at 3,122,319 to 3,123,389
_14810
Position (kb)
3121
3122
3123 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3120.763 kb on - strand at 3120.763 kb on - strand at 3120.763 kb on - strand at 3120.763 kb on - strand at 3121.117 kb on - strand at 3121.117 kb on - strand at 3121.169 kb on - strand at 3121.181 kb on - strand at 3121.181 kb on - strand at 3121.181 kb on - strand at 3121.181 kb on - strand at 3121.230 kb on - strand, within IAI47_14805 at 3121.230 kb on - strand, within IAI47_14805 at 3121.233 kb on - strand, within IAI47_14805 at 3121.271 kb on - strand, within IAI47_14805 at 3121.271 kb on - strand, within IAI47_14805 at 3121.295 kb on + strand, within IAI47_14805 at 3121.295 kb on + strand, within IAI47_14805 at 3121.295 kb on + strand, within IAI47_14805 at 3121.296 kb on - strand, within IAI47_14805 at 3121.296 kb on - strand, within IAI47_14805 at 3121.296 kb on - strand, within IAI47_14805 at 3121.296 kb on - strand, within IAI47_14805 at 3121.296 kb on - strand, within IAI47_14805 at 3121.296 kb on - strand, within IAI47_14805 at 3121.296 kb on - strand, within IAI47_14805 at 3121.296 kb on - strand, within IAI47_14805 at 3121.296 kb on - strand, within IAI47_14805 at 3121.296 kb on - strand, within IAI47_14805 at 3121.302 kb on - strand, within IAI47_14805 at 3121.312 kb on - strand, within IAI47_14805 at 3121.313 kb on + strand, within IAI47_14805 at 3121.314 kb on - strand, within IAI47_14805 at 3121.564 kb on - strand, within IAI47_14805 at 3121.564 kb on - strand, within IAI47_14805 at 3121.634 kb on - strand, within IAI47_14805 at 3121.687 kb on - strand, within IAI47_14805 at 3121.687 kb on - strand, within IAI47_14805 at 3121.707 kb on + strand, within IAI47_14805 at 3121.781 kb on + strand, within IAI47_14805 at 3121.782 kb on - strand, within IAI47_14805 at 3121.782 kb on - strand, within IAI47_14805 at 3121.806 kb on + strand, within IAI47_14805 at 3121.807 kb on - strand, within IAI47_14805 at 3121.807 kb on - strand, within IAI47_14805 at 3121.807 kb on - strand, within IAI47_14805 at 3121.807 kb on - strand, within IAI47_14805 at 3121.807 kb on - strand, within IAI47_14805 at 3121.807 kb on - strand, within IAI47_14805 at 3121.847 kb on + strand, within IAI47_14805 at 3121.905 kb on + strand, within IAI47_14805 at 3122.070 kb on + strand, within IAI47_14805 at 3122.070 kb on + strand, within IAI47_14805 at 3122.070 kb on + strand, within IAI47_14805 at 3122.070 kb on + strand, within IAI47_14805 at 3122.071 kb on - strand, within IAI47_14805 at 3122.071 kb on - strand, within IAI47_14805 at 3122.071 kb on - strand, within IAI47_14805 at 3122.071 kb on - strand, within IAI47_14805 at 3122.071 kb on - strand, within IAI47_14805 at 3122.138 kb on + strand at 3122.138 kb on + strand at 3122.139 kb on - strand at 3122.220 kb on + strand at 3122.220 kb on + strand at 3122.220 kb on + strand at 3122.220 kb on + strand at 3122.221 kb on - strand at 3122.224 kb on - strand at 3122.224 kb on - strand at 3122.271 kb on + strand at 3122.271 kb on + strand at 3122.271 kb on + strand at 3122.272 kb on - strand at 3122.272 kb on - strand at 3122.272 kb on - strand at 3122.272 kb on - strand at 3122.272 kb on - strand at 3122.272 kb on - strand at 3122.272 kb on - strand at 3122.272 kb on - strand at 3122.272 kb on - strand at 3122.272 kb on - strand at 3122.273 kb on + strand at 3122.273 kb on + strand at 3122.274 kb on - strand at 3122.274 kb on - strand at 3122.287 kb on + strand at 3122.287 kb on + strand at 3122.287 kb on + strand at 3122.288 kb on - strand at 3122.288 kb on - strand at 3122.288 kb on - strand at 3122.288 kb on - strand at 3122.290 kb on + strand at 3122.290 kb on + strand at 3122.291 kb on - strand at 3122.291 kb on - strand at 3122.315 kb on + strand at 3122.315 kb on + strand at 3122.315 kb on + strand at 3122.364 kb on - strand at 3122.380 kb on + strand at 3122.381 kb on - strand at 3122.381 kb on - strand at 3122.396 kb on - strand at 3122.419 kb on + strand at 3122.419 kb on + strand at 3122.465 kb on + strand, within IAI47_14810 at 3122.465 kb on + strand, within IAI47_14810 at 3122.465 kb on + strand, within IAI47_14810 at 3122.465 kb on + strand, within IAI47_14810 at 3122.466 kb on - strand, within IAI47_14810 at 3122.521 kb on + strand, within IAI47_14810 at 3122.521 kb on + strand, within IAI47_14810 at 3122.522 kb on - strand, within IAI47_14810 at 3122.522 kb on - strand, within IAI47_14810 at 3122.522 kb on - strand, within IAI47_14810 at 3122.522 kb on - strand, within IAI47_14810 at 3122.527 kb on + strand, within IAI47_14810 at 3122.537 kb on - strand, within IAI47_14810 at 3122.537 kb on - strand, within IAI47_14810 at 3122.646 kb on - strand, within IAI47_14810 at 3122.646 kb on - strand, within IAI47_14810 at 3122.661 kb on - strand, within IAI47_14810 at 3122.705 kb on - strand, within IAI47_14810 at 3122.885 kb on - strand, within IAI47_14810 at 3122.897 kb on - strand, within IAI47_14810 at 3122.934 kb on - strand, within IAI47_14810 at 3122.934 kb on - strand, within IAI47_14810 at 3122.934 kb on - strand, within IAI47_14810 at 3122.935 kb on + strand, within IAI47_14810 at 3122.935 kb on + strand, within IAI47_14810 at 3122.936 kb on - strand, within IAI47_14810 at 3122.936 kb on - strand, within IAI47_14810 at 3122.936 kb on - strand, within IAI47_14810 at 3122.951 kb on + strand, within IAI47_14810 at 3122.952 kb on - strand, within IAI47_14810 at 3122.952 kb on - strand, within IAI47_14810 at 3122.952 kb on - strand, within IAI47_14810 at 3122.972 kb on + strand, within IAI47_14810 at 3123.013 kb on - strand, within IAI47_14810 at 3123.126 kb on - strand, within IAI47_14810 at 3123.126 kb on - strand, within IAI47_14810 at 3123.145 kb on - strand, within IAI47_14810 at 3123.176 kb on + strand, within IAI47_14810 at 3123.177 kb on - strand, within IAI47_14810
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 3,120,763 - -2.2 3,120,763 - +0.9 3,120,763 - -0.0 3,120,763 - -0.8 3,121,117 - -0.2 3,121,117 - -0.6 3,121,169 - +0.0 3,121,181 - -1.3 3,121,181 - +0.8 3,121,181 - -0.0 3,121,181 - -0.0 3,121,230 - IAI47_14805 0.12 -0.3 3,121,230 - IAI47_14805 0.12 -1.4 3,121,233 - IAI47_14805 0.12 -2.3 3,121,271 - IAI47_14805 0.15 +2.4 3,121,271 - IAI47_14805 0.15 -0.8 3,121,295 + IAI47_14805 0.18 -0.7 3,121,295 + IAI47_14805 0.18 -1.1 3,121,295 + IAI47_14805 0.18 +0.4 3,121,296 - IAI47_14805 0.18 -1.4 3,121,296 - IAI47_14805 0.18 +1.4 3,121,296 - IAI47_14805 0.18 +1.9 3,121,296 - IAI47_14805 0.18 -0.7 3,121,296 - IAI47_14805 0.18 -2.0 3,121,296 - IAI47_14805 0.18 -0.3 3,121,296 - IAI47_14805 0.18 -0.7 3,121,296 - IAI47_14805 0.18 -0.6 3,121,296 - IAI47_14805 0.18 +0.1 3,121,296 - IAI47_14805 0.18 -0.7 3,121,302 - IAI47_14805 0.18 -0.7 3,121,312 - IAI47_14805 0.19 +0.2 3,121,313 + IAI47_14805 0.19 -1.4 3,121,314 - IAI47_14805 0.19 +1.4 3,121,564 - IAI47_14805 0.41 -0.5 3,121,564 - IAI47_14805 0.41 +0.3 3,121,634 - IAI47_14805 0.47 -2.2 3,121,687 - IAI47_14805 0.52 -2.6 3,121,687 - IAI47_14805 0.52 -1.4 3,121,707 + IAI47_14805 0.53 -1.0 3,121,781 + IAI47_14805 0.60 +2.2 3,121,782 - IAI47_14805 0.60 -1.1 3,121,782 - IAI47_14805 0.60 -1.5 3,121,806 + IAI47_14805 0.62 -1.5 3,121,807 - IAI47_14805 0.62 -0.6 3,121,807 - IAI47_14805 0.62 -2.0 3,121,807 - IAI47_14805 0.62 +0.1 3,121,807 - IAI47_14805 0.62 -1.6 3,121,807 - IAI47_14805 0.62 +2.4 3,121,807 - IAI47_14805 0.62 -2.7 3,121,847 + IAI47_14805 0.66 -0.7 3,121,905 + IAI47_14805 0.71 -2.0 3,122,070 + IAI47_14805 0.85 +0.3 3,122,070 + IAI47_14805 0.85 +0.1 3,122,070 + IAI47_14805 0.85 -0.2 3,122,070 + IAI47_14805 0.85 +0.6 3,122,071 - IAI47_14805 0.85 -0.6 3,122,071 - IAI47_14805 0.85 -1.7 3,122,071 - IAI47_14805 0.85 +0.4 3,122,071 - IAI47_14805 0.85 +0.3 3,122,071 - IAI47_14805 0.85 -1.5 3,122,138 + -0.7 3,122,138 + -0.6 3,122,139 - -0.4 3,122,220 + -0.9 3,122,220 + +0.6 3,122,220 + +4.1 3,122,220 + +0.4 3,122,221 - +0.4 3,122,224 - -0.6 3,122,224 - +0.2 3,122,271 + -0.7 3,122,271 + -0.7 3,122,271 + -1.6 3,122,272 - -1.0 3,122,272 - -1.2 3,122,272 - -0.4 3,122,272 - -0.3 3,122,272 - -1.4 3,122,272 - +0.8 3,122,272 - -1.5 3,122,272 - +0.3 3,122,272 - -0.1 3,122,272 - -0.5 3,122,273 + -0.7 3,122,273 + -0.3 3,122,274 - -0.5 3,122,274 - +0.9 3,122,287 + -1.6 3,122,287 + -1.3 3,122,287 + -0.6 3,122,288 - +0.4 3,122,288 - +0.1 3,122,288 - -0.4 3,122,288 - +1.3 3,122,290 + -0.9 3,122,290 + -0.3 3,122,291 - +0.4 3,122,291 - +2.5 3,122,315 + +1.1 3,122,315 + -0.1 3,122,315 + +0.5 3,122,364 - -0.5 3,122,380 + +0.5 3,122,381 - -1.0 3,122,381 - +0.3 3,122,396 - +0.3 3,122,419 + -1.6 3,122,419 + -1.3 3,122,465 + IAI47_14810 0.14 -0.6 3,122,465 + IAI47_14810 0.14 +0.2 3,122,465 + IAI47_14810 0.14 -0.4 3,122,465 + IAI47_14810 0.14 +0.6 3,122,466 - IAI47_14810 0.14 -0.9 3,122,521 + IAI47_14810 0.19 -0.4 3,122,521 + IAI47_14810 0.19 +3.1 3,122,522 - IAI47_14810 0.19 +1.4 3,122,522 - IAI47_14810 0.19 +0.2 3,122,522 - IAI47_14810 0.19 -1.2 3,122,522 - IAI47_14810 0.19 -0.6 3,122,527 + IAI47_14810 0.19 +1.8 3,122,537 - IAI47_14810 0.20 +0.9 3,122,537 - IAI47_14810 0.20 +1.7 3,122,646 - IAI47_14810 0.31 -1.6 3,122,646 - IAI47_14810 0.31 +1.9 3,122,661 - IAI47_14810 0.32 -1.2 3,122,705 - IAI47_14810 0.36 -2.0 3,122,885 - IAI47_14810 0.53 +0.0 3,122,897 - IAI47_14810 0.54 +0.1 3,122,934 - IAI47_14810 0.57 +0.2 3,122,934 - IAI47_14810 0.57 -0.8 3,122,934 - IAI47_14810 0.57 -0.6 3,122,935 + IAI47_14810 0.58 -0.6 3,122,935 + IAI47_14810 0.58 -0.2 3,122,936 - IAI47_14810 0.58 -2.2 3,122,936 - IAI47_14810 0.58 -2.3 3,122,936 - IAI47_14810 0.58 -1.5 3,122,951 + IAI47_14810 0.59 -2.5 3,122,952 - IAI47_14810 0.59 -0.0 3,122,952 - IAI47_14810 0.59 -1.0 3,122,952 - IAI47_14810 0.59 -0.4 3,122,972 + IAI47_14810 0.61 -1.7 3,123,013 - IAI47_14810 0.65 -0.2 3,123,126 - IAI47_14810 0.75 -0.2 3,123,126 - IAI47_14810 0.75 +1.6 3,123,145 - IAI47_14810 0.77 -0.9 3,123,176 + IAI47_14810 0.80 -1.0 3,123,177 - IAI47_14810 0.80 -1.1
Or see this region's nucleotide sequence