Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_07765 and IAI47_07770 overlap by 8 nucleotides IAI47_07770 and IAI47_07775 overlap by 11 nucleotides IAI47_07775 and IAI47_07780 are separated by 0 nucleotides
IAI47_07765: IAI47_07765 - carbon-phosphorus lyase complex subunit PhnI, at 1,644,870 to 1,645,946
_07765
IAI47_07770: IAI47_07770 - alpha-D-ribose 1-methylphosphonate 5-phosphate C-P-lyase PhnJ, at 1,645,939 to 1,646,787
_07770
IAI47_07775: IAI47_07775 - phosphonate C-P lyase system protein PhnK, at 1,646,777 to 1,647,538
_07775
IAI47_07780: IAI47_07780 - phosphonate C-P lyase system protein PhnL, at 1,647,539 to 1,648,252
_07780
Position (kb)
1645
1646
1647 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1645.030 kb on + strand, within IAI47_07765 at 1645.030 kb on + strand, within IAI47_07765 at 1645.032 kb on + strand, within IAI47_07765 at 1645.032 kb on + strand, within IAI47_07765 at 1645.032 kb on + strand, within IAI47_07765 at 1645.032 kb on + strand, within IAI47_07765 at 1645.032 kb on + strand, within IAI47_07765 at 1645.033 kb on - strand, within IAI47_07765 at 1645.033 kb on - strand, within IAI47_07765 at 1645.033 kb on - strand, within IAI47_07765 at 1645.033 kb on - strand, within IAI47_07765 at 1645.033 kb on - strand, within IAI47_07765 at 1645.061 kb on + strand, within IAI47_07765 at 1645.062 kb on - strand, within IAI47_07765 at 1645.194 kb on + strand, within IAI47_07765 at 1645.194 kb on + strand, within IAI47_07765 at 1645.194 kb on + strand, within IAI47_07765 at 1645.195 kb on - strand, within IAI47_07765 at 1645.195 kb on - strand, within IAI47_07765 at 1645.195 kb on - strand, within IAI47_07765 at 1645.197 kb on - strand, within IAI47_07765 at 1645.197 kb on - strand, within IAI47_07765 at 1645.233 kb on + strand, within IAI47_07765 at 1645.233 kb on + strand, within IAI47_07765 at 1645.233 kb on + strand, within IAI47_07765 at 1645.233 kb on + strand, within IAI47_07765 at 1645.233 kb on + strand, within IAI47_07765 at 1645.233 kb on + strand, within IAI47_07765 at 1645.234 kb on - strand, within IAI47_07765 at 1645.234 kb on - strand, within IAI47_07765 at 1645.234 kb on - strand, within IAI47_07765 at 1645.234 kb on - strand, within IAI47_07765 at 1645.239 kb on + strand, within IAI47_07765 at 1645.239 kb on + strand, within IAI47_07765 at 1645.239 kb on + strand, within IAI47_07765 at 1645.239 kb on + strand, within IAI47_07765 at 1645.239 kb on + strand, within IAI47_07765 at 1645.240 kb on - strand, within IAI47_07765 at 1645.240 kb on - strand, within IAI47_07765 at 1645.240 kb on - strand, within IAI47_07765 at 1645.240 kb on - strand, within IAI47_07765 at 1645.240 kb on - strand, within IAI47_07765 at 1645.404 kb on - strand, within IAI47_07765 at 1645.404 kb on - strand, within IAI47_07765 at 1645.557 kb on + strand, within IAI47_07765 at 1645.924 kb on - strand at 1645.957 kb on + strand at 1645.959 kb on + strand at 1645.959 kb on + strand at 1645.959 kb on + strand at 1645.960 kb on - strand at 1645.960 kb on - strand at 1645.960 kb on - strand at 1645.960 kb on - strand at 1645.969 kb on + strand at 1645.970 kb on - strand at 1645.970 kb on - strand at 1646.074 kb on + strand, within IAI47_07770 at 1646.086 kb on - strand, within IAI47_07770 at 1646.183 kb on + strand, within IAI47_07770 at 1646.183 kb on + strand, within IAI47_07770 at 1646.183 kb on + strand, within IAI47_07770 at 1646.183 kb on + strand, within IAI47_07770 at 1646.184 kb on - strand, within IAI47_07770 at 1646.303 kb on + strand, within IAI47_07770 at 1646.386 kb on + strand, within IAI47_07770 at 1646.387 kb on - strand, within IAI47_07770 at 1646.387 kb on - strand, within IAI47_07770 at 1646.410 kb on + strand, within IAI47_07770 at 1646.410 kb on + strand, within IAI47_07770 at 1646.411 kb on - strand, within IAI47_07770 at 1646.411 kb on - strand, within IAI47_07770 at 1646.418 kb on + strand, within IAI47_07770 at 1646.419 kb on - strand, within IAI47_07770 at 1646.419 kb on - strand, within IAI47_07770 at 1646.437 kb on - strand, within IAI47_07770 at 1646.449 kb on + strand, within IAI47_07770 at 1646.449 kb on + strand, within IAI47_07770 at 1646.449 kb on + strand, within IAI47_07770 at 1646.449 kb on + strand, within IAI47_07770 at 1646.449 kb on + strand, within IAI47_07770 at 1646.449 kb on + strand, within IAI47_07770 at 1646.450 kb on - strand, within IAI47_07770 at 1646.450 kb on - strand, within IAI47_07770 at 1646.450 kb on - strand, within IAI47_07770 at 1646.450 kb on - strand, within IAI47_07770 at 1646.450 kb on - strand, within IAI47_07770 at 1646.450 kb on - strand, within IAI47_07770 at 1646.450 kb on - strand, within IAI47_07770 at 1646.450 kb on - strand, within IAI47_07770 at 1646.479 kb on + strand, within IAI47_07770 at 1646.479 kb on + strand, within IAI47_07770 at 1646.480 kb on - strand, within IAI47_07770 at 1646.578 kb on + strand, within IAI47_07770 at 1646.579 kb on - strand, within IAI47_07770 at 1646.579 kb on - strand, within IAI47_07770 at 1646.579 kb on - strand, within IAI47_07770 at 1646.593 kb on + strand, within IAI47_07770 at 1646.593 kb on + strand, within IAI47_07770 at 1646.594 kb on - strand, within IAI47_07770 at 1646.595 kb on + strand, within IAI47_07770 at 1646.686 kb on + strand, within IAI47_07770 at 1646.686 kb on + strand, within IAI47_07770 at 1646.686 kb on + strand, within IAI47_07770 at 1646.687 kb on - strand, within IAI47_07770 at 1646.687 kb on - strand, within IAI47_07770 at 1646.687 kb on - strand, within IAI47_07770 at 1646.687 kb on - strand, within IAI47_07770 at 1646.825 kb on + strand at 1646.825 kb on + strand at 1646.825 kb on + strand at 1646.826 kb on - strand at 1646.826 kb on - strand at 1646.826 kb on - strand at 1646.826 kb on - strand at 1646.880 kb on + strand, within IAI47_07775 at 1646.880 kb on + strand, within IAI47_07775 at 1646.881 kb on - strand, within IAI47_07775 at 1646.881 kb on - strand, within IAI47_07775 at 1646.912 kb on - strand, within IAI47_07775 at 1646.972 kb on + strand, within IAI47_07775 at 1646.973 kb on - strand, within IAI47_07775 at 1646.991 kb on - strand, within IAI47_07775 at 1647.134 kb on + strand, within IAI47_07775 at 1647.134 kb on + strand, within IAI47_07775 at 1647.134 kb on + strand, within IAI47_07775 at 1647.287 kb on - strand, within IAI47_07775 at 1647.287 kb on - strand, within IAI47_07775 at 1647.334 kb on - strand, within IAI47_07775 at 1647.503 kb on + strand at 1647.503 kb on + strand at 1647.503 kb on + strand at 1647.503 kb on + strand at 1647.504 kb on - strand at 1647.504 kb on - strand at 1647.504 kb on - strand at 1647.536 kb on + strand at 1647.536 kb on + strand at 1647.580 kb on + strand at 1647.581 kb on - strand at 1647.670 kb on - strand, within IAI47_07780 at 1647.670 kb on - strand, within IAI47_07780 at 1647.728 kb on + strand, within IAI47_07780 at 1647.729 kb on - strand, within IAI47_07780 at 1647.729 kb on - strand, within IAI47_07780 at 1647.729 kb on - strand, within IAI47_07780
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 1,645,030 + IAI47_07765 0.15 -0.2 1,645,030 + IAI47_07765 0.15 +0.5 1,645,032 + IAI47_07765 0.15 +1.3 1,645,032 + IAI47_07765 0.15 +0.5 1,645,032 + IAI47_07765 0.15 -1.1 1,645,032 + IAI47_07765 0.15 -1.4 1,645,032 + IAI47_07765 0.15 +0.3 1,645,033 - IAI47_07765 0.15 +0.3 1,645,033 - IAI47_07765 0.15 +0.2 1,645,033 - IAI47_07765 0.15 -1.4 1,645,033 - IAI47_07765 0.15 -1.2 1,645,033 - IAI47_07765 0.15 +0.2 1,645,061 + IAI47_07765 0.18 +1.1 1,645,062 - IAI47_07765 0.18 -0.3 1,645,194 + IAI47_07765 0.30 +0.7 1,645,194 + IAI47_07765 0.30 -1.2 1,645,194 + IAI47_07765 0.30 +1.3 1,645,195 - IAI47_07765 0.30 -1.7 1,645,195 - IAI47_07765 0.30 -0.2 1,645,195 - IAI47_07765 0.30 -0.4 1,645,197 - IAI47_07765 0.30 -1.1 1,645,197 - IAI47_07765 0.30 +0.3 1,645,233 + IAI47_07765 0.34 +0.0 1,645,233 + IAI47_07765 0.34 -0.0 1,645,233 + IAI47_07765 0.34 +0.1 1,645,233 + IAI47_07765 0.34 -2.0 1,645,233 + IAI47_07765 0.34 +1.6 1,645,233 + IAI47_07765 0.34 -0.7 1,645,234 - IAI47_07765 0.34 -0.2 1,645,234 - IAI47_07765 0.34 -1.3 1,645,234 - IAI47_07765 0.34 -0.0 1,645,234 - IAI47_07765 0.34 +1.6 1,645,239 + IAI47_07765 0.34 +0.6 1,645,239 + IAI47_07765 0.34 +0.7 1,645,239 + IAI47_07765 0.34 +1.0 1,645,239 + IAI47_07765 0.34 +2.3 1,645,239 + IAI47_07765 0.34 -0.5 1,645,240 - IAI47_07765 0.34 -0.1 1,645,240 - IAI47_07765 0.34 +0.9 1,645,240 - IAI47_07765 0.34 +0.0 1,645,240 - IAI47_07765 0.34 -1.0 1,645,240 - IAI47_07765 0.34 -0.3 1,645,404 - IAI47_07765 0.50 -1.4 1,645,404 - IAI47_07765 0.50 -0.2 1,645,557 + IAI47_07765 0.64 +0.1 1,645,924 - -0.1 1,645,957 + -0.6 1,645,959 + -1.1 1,645,959 + -0.7 1,645,959 + +0.3 1,645,960 - -0.8 1,645,960 - -0.7 1,645,960 - +1.1 1,645,960 - +0.2 1,645,969 + -1.2 1,645,970 - -0.4 1,645,970 - +0.7 1,646,074 + IAI47_07770 0.16 +1.9 1,646,086 - IAI47_07770 0.17 +1.9 1,646,183 + IAI47_07770 0.29 +0.9 1,646,183 + IAI47_07770 0.29 -0.2 1,646,183 + IAI47_07770 0.29 +0.3 1,646,183 + IAI47_07770 0.29 -1.6 1,646,184 - IAI47_07770 0.29 -0.7 1,646,303 + IAI47_07770 0.43 -2.7 1,646,386 + IAI47_07770 0.53 +0.5 1,646,387 - IAI47_07770 0.53 -2.2 1,646,387 - IAI47_07770 0.53 +1.6 1,646,410 + IAI47_07770 0.55 +3.3 1,646,410 + IAI47_07770 0.55 +0.3 1,646,411 - IAI47_07770 0.56 +2.3 1,646,411 - IAI47_07770 0.56 +0.6 1,646,418 + IAI47_07770 0.56 -0.8 1,646,419 - IAI47_07770 0.57 -0.2 1,646,419 - IAI47_07770 0.57 +0.5 1,646,437 - IAI47_07770 0.59 +1.6 1,646,449 + IAI47_07770 0.60 +1.1 1,646,449 + IAI47_07770 0.60 +0.8 1,646,449 + IAI47_07770 0.60 +1.2 1,646,449 + IAI47_07770 0.60 +0.5 1,646,449 + IAI47_07770 0.60 -0.2 1,646,449 + IAI47_07770 0.60 -0.7 1,646,450 - IAI47_07770 0.60 +1.8 1,646,450 - IAI47_07770 0.60 +1.1 1,646,450 - IAI47_07770 0.60 +0.3 1,646,450 - IAI47_07770 0.60 +1.1 1,646,450 - IAI47_07770 0.60 +0.9 1,646,450 - IAI47_07770 0.60 +1.6 1,646,450 - IAI47_07770 0.60 -0.9 1,646,450 - IAI47_07770 0.60 +1.8 1,646,479 + IAI47_07770 0.64 +2.3 1,646,479 + IAI47_07770 0.64 +0.3 1,646,480 - IAI47_07770 0.64 -0.1 1,646,578 + IAI47_07770 0.75 +0.0 1,646,579 - IAI47_07770 0.75 -1.2 1,646,579 - IAI47_07770 0.75 -1.7 1,646,579 - IAI47_07770 0.75 +0.5 1,646,593 + IAI47_07770 0.77 -0.2 1,646,593 + IAI47_07770 0.77 +1.4 1,646,594 - IAI47_07770 0.77 -1.2 1,646,595 + IAI47_07770 0.77 +1.0 1,646,686 + IAI47_07770 0.88 +0.9 1,646,686 + IAI47_07770 0.88 -0.7 1,646,686 + IAI47_07770 0.88 +0.3 1,646,687 - IAI47_07770 0.88 +0.3 1,646,687 - IAI47_07770 0.88 +0.6 1,646,687 - IAI47_07770 0.88 -1.4 1,646,687 - IAI47_07770 0.88 +1.2 1,646,825 + +2.3 1,646,825 + +0.8 1,646,825 + -0.4 1,646,826 - -2.2 1,646,826 - +1.1 1,646,826 - -0.2 1,646,826 - -1.2 1,646,880 + IAI47_07775 0.14 -1.8 1,646,880 + IAI47_07775 0.14 -0.0 1,646,881 - IAI47_07775 0.14 +0.5 1,646,881 - IAI47_07775 0.14 -1.0 1,646,912 - IAI47_07775 0.18 -0.6 1,646,972 + IAI47_07775 0.26 +0.3 1,646,973 - IAI47_07775 0.26 +1.5 1,646,991 - IAI47_07775 0.28 -1.0 1,647,134 + IAI47_07775 0.47 +1.1 1,647,134 + IAI47_07775 0.47 +0.1 1,647,134 + IAI47_07775 0.47 +0.4 1,647,287 - IAI47_07775 0.67 -0.5 1,647,287 - IAI47_07775 0.67 +0.0 1,647,334 - IAI47_07775 0.73 +0.8 1,647,503 + -1.6 1,647,503 + +1.8 1,647,503 + +0.5 1,647,503 + -1.0 1,647,504 - -0.7 1,647,504 - -1.0 1,647,504 - +2.1 1,647,536 + +1.5 1,647,536 + -0.8 1,647,580 + -1.1 1,647,581 - +0.2 1,647,670 - IAI47_07780 0.18 -0.1 1,647,670 - IAI47_07780 0.18 -2.2 1,647,728 + IAI47_07780 0.26 +2.6 1,647,729 - IAI47_07780 0.27 -1.8 1,647,729 - IAI47_07780 0.27 -0.1 1,647,729 - IAI47_07780 0.27 +0.1
Or see this region's nucleotide sequence