Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_21295 and IAI47_21300 are separated by 103 nucleotides IAI47_21300 and IAI47_21305 overlap by 4 nucleotides IAI47_21305 and IAI47_21310 are separated by 111 nucleotides IAI47_21310 and IAI47_21315 are separated by 0 nucleotides
IAI47_21295: IAI47_21295 - DJ-1/PfpI family protein, at 514,830 to 515,549
_21295
IAI47_21300: IAI47_21300 - AzlD domain-containing protein, at 515,653 to 515,976
_21300
IAI47_21305: IAI47_21305 - AzlC family ABC transporter permease, at 515,973 to 516,641
_21305
IAI47_21310: IAI47_21310 - helix-turn-helix domain-containing protein, at 516,753 to 517,301
_21310
IAI47_21315: IAI47_21315 - hypothetical protein, at 517,302 to 517,640
_21315
Position (kb) 515
516
517 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 515.002 kb on + strand, within IAI47_21295 at 515.002 kb on + strand, within IAI47_21295 at 515.002 kb on + strand, within IAI47_21295 at 515.003 kb on - strand, within IAI47_21295 at 515.013 kb on + strand, within IAI47_21295 at 515.014 kb on - strand, within IAI47_21295 at 515.014 kb on - strand, within IAI47_21295 at 515.204 kb on + strand, within IAI47_21295 at 515.204 kb on + strand, within IAI47_21295 at 515.205 kb on - strand, within IAI47_21295 at 515.205 kb on - strand, within IAI47_21295 at 515.241 kb on - strand, within IAI47_21295 at 515.252 kb on + strand, within IAI47_21295 at 515.252 kb on + strand, within IAI47_21295 at 515.252 kb on + strand, within IAI47_21295 at 515.252 kb on + strand, within IAI47_21295 at 515.252 kb on + strand, within IAI47_21295 at 515.252 kb on + strand, within IAI47_21295 at 515.252 kb on + strand, within IAI47_21295 at 515.253 kb on - strand, within IAI47_21295 at 515.253 kb on - strand, within IAI47_21295 at 515.253 kb on - strand, within IAI47_21295 at 515.253 kb on - strand, within IAI47_21295 at 515.253 kb on - strand, within IAI47_21295 at 515.253 kb on - strand, within IAI47_21295 at 515.303 kb on + strand, within IAI47_21295 at 515.304 kb on - strand, within IAI47_21295 at 515.369 kb on + strand, within IAI47_21295 at 515.369 kb on + strand, within IAI47_21295 at 515.369 kb on + strand, within IAI47_21295 at 515.370 kb on - strand, within IAI47_21295 at 515.370 kb on - strand, within IAI47_21295 at 515.370 kb on - strand, within IAI47_21295 at 515.370 kb on - strand, within IAI47_21295 at 515.370 kb on - strand, within IAI47_21295 at 515.370 kb on - strand, within IAI47_21295 at 515.370 kb on - strand, within IAI47_21295 at 515.370 kb on - strand, within IAI47_21295 at 515.392 kb on - strand, within IAI47_21295 at 515.520 kb on + strand at 515.521 kb on - strand at 515.521 kb on - strand at 515.521 kb on - strand at 515.549 kb on + strand at 515.549 kb on + strand at 515.549 kb on + strand at 515.549 kb on + strand at 515.590 kb on + strand at 515.604 kb on + strand at 515.604 kb on + strand at 515.604 kb on + strand at 515.605 kb on - strand at 515.605 kb on - strand at 515.628 kb on + strand at 515.628 kb on + strand at 515.629 kb on - strand at 515.660 kb on - strand at 515.674 kb on + strand at 515.674 kb on + strand at 515.674 kb on + strand at 515.740 kb on + strand, within IAI47_21300 at 515.740 kb on + strand, within IAI47_21300 at 515.740 kb on + strand, within IAI47_21300 at 515.741 kb on - strand, within IAI47_21300 at 515.743 kb on + strand, within IAI47_21300 at 515.854 kb on + strand, within IAI47_21300 at 515.854 kb on + strand, within IAI47_21300 at 515.854 kb on + strand, within IAI47_21300 at 515.855 kb on - strand, within IAI47_21300 at 515.921 kb on + strand, within IAI47_21300 at 515.921 kb on + strand, within IAI47_21300 at 515.921 kb on + strand, within IAI47_21300 at 515.921 kb on + strand, within IAI47_21300 at 515.921 kb on + strand, within IAI47_21300 at 515.921 kb on + strand, within IAI47_21300 at 515.921 kb on + strand, within IAI47_21300 at 515.940 kb on + strand, within IAI47_21300 at 515.940 kb on + strand, within IAI47_21300 at 515.940 kb on + strand, within IAI47_21300 at 515.940 kb on + strand, within IAI47_21300 at 515.940 kb on + strand, within IAI47_21300 at 515.940 kb on + strand, within IAI47_21300 at 515.941 kb on - strand, within IAI47_21300 at 515.941 kb on - strand, within IAI47_21300 at 515.941 kb on - strand, within IAI47_21300 at 516.063 kb on + strand, within IAI47_21305 at 516.064 kb on - strand, within IAI47_21305 at 516.224 kb on + strand, within IAI47_21305 at 516.366 kb on + strand, within IAI47_21305 at 516.550 kb on + strand, within IAI47_21305 at 516.550 kb on + strand, within IAI47_21305 at 516.551 kb on - strand, within IAI47_21305 at 516.551 kb on - strand, within IAI47_21305 at 516.555 kb on + strand, within IAI47_21305 at 516.556 kb on - strand, within IAI47_21305 at 516.556 kb on - strand, within IAI47_21305 at 516.556 kb on - strand, within IAI47_21305 at 516.556 kb on - strand, within IAI47_21305 at 516.599 kb on + strand at 516.600 kb on - strand at 516.600 kb on - strand at 516.603 kb on + strand at 516.604 kb on - strand at 516.665 kb on + strand at 516.674 kb on - strand at 516.675 kb on + strand at 516.675 kb on + strand at 516.764 kb on + strand at 516.773 kb on + strand at 516.773 kb on + strand at 516.774 kb on - strand at 516.774 kb on - strand at 516.774 kb on - strand at 516.774 kb on - strand at 516.774 kb on - strand at 516.787 kb on + strand at 516.787 kb on + strand at 516.788 kb on - strand at 516.830 kb on - strand, within IAI47_21310 at 516.830 kb on - strand, within IAI47_21310 at 516.857 kb on + strand, within IAI47_21310 at 516.858 kb on - strand, within IAI47_21310 at 516.858 kb on - strand, within IAI47_21310 at 516.858 kb on - strand, within IAI47_21310 at 516.858 kb on - strand, within IAI47_21310 at 516.887 kb on + strand, within IAI47_21310 at 516.933 kb on - strand, within IAI47_21310 at 516.933 kb on - strand, within IAI47_21310 at 516.982 kb on + strand, within IAI47_21310 at 516.982 kb on + strand, within IAI47_21310 at 516.983 kb on - strand, within IAI47_21310 at 516.983 kb on - strand, within IAI47_21310 at 516.983 kb on - strand, within IAI47_21310 at 516.983 kb on - strand, within IAI47_21310 at 517.026 kb on + strand, within IAI47_21310 at 517.073 kb on + strand, within IAI47_21310 at 517.073 kb on + strand, within IAI47_21310 at 517.073 kb on + strand, within IAI47_21310 at 517.073 kb on + strand, within IAI47_21310 at 517.074 kb on - strand, within IAI47_21310 at 517.074 kb on - strand, within IAI47_21310 at 517.077 kb on + strand, within IAI47_21310 at 517.077 kb on + strand, within IAI47_21310 at 517.078 kb on - strand, within IAI47_21310 at 517.133 kb on + strand, within IAI47_21310 at 517.133 kb on + strand, within IAI47_21310 at 517.145 kb on + strand, within IAI47_21310 at 517.145 kb on + strand, within IAI47_21310 at 517.145 kb on + strand, within IAI47_21310 at 517.146 kb on - strand, within IAI47_21310 at 517.146 kb on - strand, within IAI47_21310 at 517.146 kb on - strand, within IAI47_21310 at 517.146 kb on - strand, within IAI47_21310 at 517.148 kb on + strand, within IAI47_21310 at 517.148 kb on + strand, within IAI47_21310 at 517.148 kb on + strand, within IAI47_21310 at 517.148 kb on + strand, within IAI47_21310 at 517.148 kb on + strand, within IAI47_21310 at 517.148 kb on + strand, within IAI47_21310 at 517.148 kb on + strand, within IAI47_21310 at 517.148 kb on + strand, within IAI47_21310 at 517.149 kb on - strand, within IAI47_21310 at 517.149 kb on - strand, within IAI47_21310 at 517.149 kb on - strand, within IAI47_21310 at 517.149 kb on - strand, within IAI47_21310 at 517.149 kb on - strand, within IAI47_21310 at 517.215 kb on + strand, within IAI47_21310 at 517.215 kb on + strand, within IAI47_21310 at 517.215 kb on + strand, within IAI47_21310 at 517.215 kb on + strand, within IAI47_21310 at 517.215 kb on + strand, within IAI47_21310 at 517.215 kb on + strand, within IAI47_21310 at 517.216 kb on - strand, within IAI47_21310 at 517.216 kb on - strand, within IAI47_21310 at 517.216 kb on - strand, within IAI47_21310 at 517.216 kb on - strand, within IAI47_21310 at 517.216 kb on - strand, within IAI47_21310 at 517.216 kb on - strand, within IAI47_21310 at 517.216 kb on - strand, within IAI47_21310 at 517.220 kb on + strand, within IAI47_21310 at 517.220 kb on + strand, within IAI47_21310 at 517.220 kb on + strand, within IAI47_21310 at 517.220 kb on + strand, within IAI47_21310 at 517.220 kb on + strand, within IAI47_21310 at 517.221 kb on - strand, within IAI47_21310 at 517.221 kb on - strand, within IAI47_21310 at 517.221 kb on - strand, within IAI47_21310 at 517.221 kb on - strand, within IAI47_21310 at 517.221 kb on - strand, within IAI47_21310 at 517.221 kb on - strand, within IAI47_21310 at 517.224 kb on + strand, within IAI47_21310 at 517.224 kb on + strand, within IAI47_21310 at 517.225 kb on - strand, within IAI47_21310 at 517.244 kb on + strand, within IAI47_21310 at 517.268 kb on + strand at 517.299 kb on + strand at 517.299 kb on + strand at 517.299 kb on + strand at 517.299 kb on + strand at 517.299 kb on + strand at 517.299 kb on + strand at 517.300 kb on - strand at 517.300 kb on - strand at 517.300 kb on - strand at 517.303 kb on + strand at 517.304 kb on - strand at 517.320 kb on + strand at 517.320 kb on + strand at 517.321 kb on - strand at 517.321 kb on - strand at 517.387 kb on + strand, within IAI47_21315 at 517.388 kb on - strand, within IAI47_21315 at 517.494 kb on + strand, within IAI47_21315 at 517.495 kb on - strand, within IAI47_21315 at 517.495 kb on - strand, within IAI47_21315 at 517.495 kb on - strand, within IAI47_21315 at 517.495 kb on - strand, within IAI47_21315 at 517.495 kb on - strand, within IAI47_21315 at 517.495 kb on - strand, within IAI47_21315 at 517.500 kb on + strand, within IAI47_21315 at 517.500 kb on + strand, within IAI47_21315 at 517.500 kb on + strand, within IAI47_21315 at 517.501 kb on - strand, within IAI47_21315 at 517.501 kb on - strand, within IAI47_21315 at 517.501 kb on - strand, within IAI47_21315 at 517.501 kb on - strand, within IAI47_21315 at 517.501 kb on - strand, within IAI47_21315 at 517.572 kb on + strand, within IAI47_21315 at 517.572 kb on + strand, within IAI47_21315 at 517.573 kb on - strand, within IAI47_21315
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 515,002 + IAI47_21295 0.24 -0.1 515,002 + IAI47_21295 0.24 -0.2 515,002 + IAI47_21295 0.24 -1.2 515,003 - IAI47_21295 0.24 +1.1 515,013 + IAI47_21295 0.25 -2.7 515,014 - IAI47_21295 0.26 +0.5 515,014 - IAI47_21295 0.26 +0.1 515,204 + IAI47_21295 0.52 +0.2 515,204 + IAI47_21295 0.52 -1.9 515,205 - IAI47_21295 0.52 -1.7 515,205 - IAI47_21295 0.52 +0.4 515,241 - IAI47_21295 0.57 +0.8 515,252 + IAI47_21295 0.59 +0.1 515,252 + IAI47_21295 0.59 +0.4 515,252 + IAI47_21295 0.59 -1.2 515,252 + IAI47_21295 0.59 -2.4 515,252 + IAI47_21295 0.59 +0.2 515,252 + IAI47_21295 0.59 +0.4 515,252 + IAI47_21295 0.59 +0.1 515,253 - IAI47_21295 0.59 -2.8 515,253 - IAI47_21295 0.59 -0.8 515,253 - IAI47_21295 0.59 +0.1 515,253 - IAI47_21295 0.59 -2.3 515,253 - IAI47_21295 0.59 -0.4 515,253 - IAI47_21295 0.59 -0.0 515,303 + IAI47_21295 0.66 -0.8 515,304 - IAI47_21295 0.66 +1.7 515,369 + IAI47_21295 0.75 +0.3 515,369 + IAI47_21295 0.75 +0.4 515,369 + IAI47_21295 0.75 -0.4 515,370 - IAI47_21295 0.75 -0.7 515,370 - IAI47_21295 0.75 -2.1 515,370 - IAI47_21295 0.75 +1.2 515,370 - IAI47_21295 0.75 -0.4 515,370 - IAI47_21295 0.75 +0.8 515,370 - IAI47_21295 0.75 +0.1 515,370 - IAI47_21295 0.75 -0.4 515,370 - IAI47_21295 0.75 +0.1 515,392 - IAI47_21295 0.78 +0.2 515,520 + +1.0 515,521 - -0.2 515,521 - +0.3 515,521 - -1.9 515,549 + -0.6 515,549 + -2.2 515,549 + +1.3 515,549 + -1.0 515,590 + +0.1 515,604 + +0.3 515,604 + +1.8 515,604 + -0.2 515,605 - -0.1 515,605 - +0.3 515,628 + -0.2 515,628 + -0.9 515,629 - +1.3 515,660 - +0.2 515,674 + -0.8 515,674 + +1.4 515,674 + -2.0 515,740 + IAI47_21300 0.27 +1.1 515,740 + IAI47_21300 0.27 -0.9 515,740 + IAI47_21300 0.27 -1.5 515,741 - IAI47_21300 0.27 -2.6 515,743 + IAI47_21300 0.28 -1.9 515,854 + IAI47_21300 0.62 +0.4 515,854 + IAI47_21300 0.62 -0.1 515,854 + IAI47_21300 0.62 +0.1 515,855 - IAI47_21300 0.62 -1.9 515,921 + IAI47_21300 0.83 -0.6 515,921 + IAI47_21300 0.83 -0.1 515,921 + IAI47_21300 0.83 -0.0 515,921 + IAI47_21300 0.83 +1.1 515,921 + IAI47_21300 0.83 +0.1 515,921 + IAI47_21300 0.83 -0.3 515,921 + IAI47_21300 0.83 -0.9 515,940 + IAI47_21300 0.89 +0.4 515,940 + IAI47_21300 0.89 +1.0 515,940 + IAI47_21300 0.89 +0.2 515,940 + IAI47_21300 0.89 -1.6 515,940 + IAI47_21300 0.89 -0.2 515,940 + IAI47_21300 0.89 +1.4 515,941 - IAI47_21300 0.89 -0.6 515,941 - IAI47_21300 0.89 -0.5 515,941 - IAI47_21300 0.89 -2.7 516,063 + IAI47_21305 0.13 -0.0 516,064 - IAI47_21305 0.14 +1.6 516,224 + IAI47_21305 0.38 +2.0 516,366 + IAI47_21305 0.59 +0.5 516,550 + IAI47_21305 0.86 +1.9 516,550 + IAI47_21305 0.86 +1.4 516,551 - IAI47_21305 0.86 +0.5 516,551 - IAI47_21305 0.86 +3.2 516,555 + IAI47_21305 0.87 +0.8 516,556 - IAI47_21305 0.87 +1.1 516,556 - IAI47_21305 0.87 +1.0 516,556 - IAI47_21305 0.87 -1.2 516,556 - IAI47_21305 0.87 +1.3 516,599 + -1.9 516,600 - +0.4 516,600 - +1.0 516,603 + +0.9 516,604 - +1.1 516,665 + +0.3 516,674 - +1.8 516,675 + +0.7 516,675 + +1.6 516,764 + -1.2 516,773 + -2.3 516,773 + +0.2 516,774 - -0.9 516,774 - -1.6 516,774 - -0.8 516,774 - -1.1 516,774 - -1.8 516,787 + -1.1 516,787 + -0.7 516,788 - -1.4 516,830 - IAI47_21310 0.14 -0.6 516,830 - IAI47_21310 0.14 +0.8 516,857 + IAI47_21310 0.19 +0.7 516,858 - IAI47_21310 0.19 -0.9 516,858 - IAI47_21310 0.19 -0.6 516,858 - IAI47_21310 0.19 -2.5 516,858 - IAI47_21310 0.19 +1.1 516,887 + IAI47_21310 0.24 -2.0 516,933 - IAI47_21310 0.33 -0.3 516,933 - IAI47_21310 0.33 +0.1 516,982 + IAI47_21310 0.42 +0.2 516,982 + IAI47_21310 0.42 -0.4 516,983 - IAI47_21310 0.42 -1.6 516,983 - IAI47_21310 0.42 +0.9 516,983 - IAI47_21310 0.42 -1.0 516,983 - IAI47_21310 0.42 -0.9 517,026 + IAI47_21310 0.50 -0.1 517,073 + IAI47_21310 0.58 -1.5 517,073 + IAI47_21310 0.58 +1.8 517,073 + IAI47_21310 0.58 +3.3 517,073 + IAI47_21310 0.58 +1.6 517,074 - IAI47_21310 0.58 +0.1 517,074 - IAI47_21310 0.58 -0.7 517,077 + IAI47_21310 0.59 +1.6 517,077 + IAI47_21310 0.59 -1.7 517,078 - IAI47_21310 0.59 -0.7 517,133 + IAI47_21310 0.69 -1.1 517,133 + IAI47_21310 0.69 -1.7 517,145 + IAI47_21310 0.71 -0.9 517,145 + IAI47_21310 0.71 -2.0 517,145 + IAI47_21310 0.71 +0.4 517,146 - IAI47_21310 0.72 +0.6 517,146 - IAI47_21310 0.72 +0.4 517,146 - IAI47_21310 0.72 +1.0 517,146 - IAI47_21310 0.72 +0.3 517,148 + IAI47_21310 0.72 -1.0 517,148 + IAI47_21310 0.72 -0.2 517,148 + IAI47_21310 0.72 +1.7 517,148 + IAI47_21310 0.72 -0.5 517,148 + IAI47_21310 0.72 -1.2 517,148 + IAI47_21310 0.72 -1.1 517,148 + IAI47_21310 0.72 +1.5 517,148 + IAI47_21310 0.72 +0.4 517,149 - IAI47_21310 0.72 +1.8 517,149 - IAI47_21310 0.72 -0.7 517,149 - IAI47_21310 0.72 -1.9 517,149 - IAI47_21310 0.72 +2.1 517,149 - IAI47_21310 0.72 +0.7 517,215 + IAI47_21310 0.84 +0.5 517,215 + IAI47_21310 0.84 +0.8 517,215 + IAI47_21310 0.84 +1.3 517,215 + IAI47_21310 0.84 +1.0 517,215 + IAI47_21310 0.84 -1.7 517,215 + IAI47_21310 0.84 -0.6 517,216 - IAI47_21310 0.84 -0.6 517,216 - IAI47_21310 0.84 -0.8 517,216 - IAI47_21310 0.84 +1.1 517,216 - IAI47_21310 0.84 +0.4 517,216 - IAI47_21310 0.84 -0.2 517,216 - IAI47_21310 0.84 +0.7 517,216 - IAI47_21310 0.84 -1.9 517,220 + IAI47_21310 0.85 +0.4 517,220 + IAI47_21310 0.85 +1.6 517,220 + IAI47_21310 0.85 -0.5 517,220 + IAI47_21310 0.85 -0.6 517,220 + IAI47_21310 0.85 -1.4 517,221 - IAI47_21310 0.85 +1.0 517,221 - IAI47_21310 0.85 +0.0 517,221 - IAI47_21310 0.85 -1.4 517,221 - IAI47_21310 0.85 -1.2 517,221 - IAI47_21310 0.85 +0.8 517,221 - IAI47_21310 0.85 -0.1 517,224 + IAI47_21310 0.86 -2.1 517,224 + IAI47_21310 0.86 -1.4 517,225 - IAI47_21310 0.86 +0.2 517,244 + IAI47_21310 0.89 -1.3 517,268 + +2.6 517,299 + -1.9 517,299 + +0.6 517,299 + +0.4 517,299 + +0.3 517,299 + -1.4 517,299 + +0.4 517,300 - +0.3 517,300 - +0.1 517,300 - +0.3 517,303 + +0.4 517,304 - +0.8 517,320 + +0.8 517,320 + +0.3 517,321 - +0.6 517,321 - +1.0 517,387 + IAI47_21315 0.25 +0.1 517,388 - IAI47_21315 0.25 +0.4 517,494 + IAI47_21315 0.57 +0.8 517,495 - IAI47_21315 0.57 +0.1 517,495 - IAI47_21315 0.57 +0.1 517,495 - IAI47_21315 0.57 +0.9 517,495 - IAI47_21315 0.57 +1.2 517,495 - IAI47_21315 0.57 -0.1 517,495 - IAI47_21315 0.57 +0.1 517,500 + IAI47_21315 0.58 -1.4 517,500 + IAI47_21315 0.58 +1.1 517,500 + IAI47_21315 0.58 -1.0 517,501 - IAI47_21315 0.59 -0.7 517,501 - IAI47_21315 0.59 -1.2 517,501 - IAI47_21315 0.59 -0.3 517,501 - IAI47_21315 0.59 +0.8 517,501 - IAI47_21315 0.59 -1.8 517,572 + IAI47_21315 0.80 -1.9 517,572 + IAI47_21315 0.80 +0.8 517,573 - IAI47_21315 0.80 +0.4
Or see this region's nucleotide sequence