Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_14725 and IAI47_14730 are separated by 232 nucleotides IAI47_14730 and IAI47_14735 overlap by 1 nucleotides IAI47_14735 and IAI47_14740 are separated by 19 nucleotides
IAI47_14725: IAI47_14725 - tRNA 4-thiouridine(8) synthase ThiI, at 3,106,740 to 3,108,188
_14725
IAI47_14730: IAI47_14730 - exodeoxyribonuclease VII small subunit, at 3,108,421 to 3,108,663
_14730
IAI47_14735: IAI47_14735 - (2E,6E)-farnesyl diphosphate synthase, at 3,108,663 to 3,109,562
_14735
IAI47_14740: IAI47_14740 - 1-deoxy-D-xylulose-5-phosphate synthase, at 3,109,582 to 3,111,447
_14740
Position (kb)
3108
3109 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3107.470 kb on - strand, within IAI47_14725 at 3107.470 kb on - strand, within IAI47_14725 at 3107.470 kb on - strand, within IAI47_14725 at 3107.479 kb on - strand, within IAI47_14725 at 3107.512 kb on + strand, within IAI47_14725 at 3107.512 kb on + strand, within IAI47_14725 at 3107.512 kb on + strand, within IAI47_14725 at 3107.512 kb on + strand, within IAI47_14725 at 3107.513 kb on - strand, within IAI47_14725 at 3107.513 kb on - strand, within IAI47_14725 at 3107.513 kb on - strand, within IAI47_14725 at 3107.513 kb on - strand, within IAI47_14725 at 3107.561 kb on + strand, within IAI47_14725 at 3107.572 kb on + strand, within IAI47_14725 at 3107.572 kb on + strand, within IAI47_14725 at 3107.603 kb on + strand, within IAI47_14725 at 3107.603 kb on + strand, within IAI47_14725 at 3107.603 kb on + strand, within IAI47_14725 at 3107.603 kb on + strand, within IAI47_14725 at 3107.603 kb on + strand, within IAI47_14725 at 3107.604 kb on - strand, within IAI47_14725 at 3107.604 kb on - strand, within IAI47_14725 at 3107.604 kb on - strand, within IAI47_14725 at 3107.604 kb on - strand, within IAI47_14725 at 3107.605 kb on + strand, within IAI47_14725 at 3107.605 kb on + strand, within IAI47_14725 at 3107.605 kb on + strand, within IAI47_14725 at 3107.605 kb on + strand, within IAI47_14725 at 3107.605 kb on + strand, within IAI47_14725 at 3107.605 kb on + strand, within IAI47_14725 at 3107.605 kb on + strand, within IAI47_14725 at 3107.605 kb on + strand, within IAI47_14725 at 3107.606 kb on - strand, within IAI47_14725 at 3107.606 kb on - strand, within IAI47_14725 at 3107.606 kb on - strand, within IAI47_14725 at 3107.606 kb on - strand, within IAI47_14725 at 3107.606 kb on - strand, within IAI47_14725 at 3107.606 kb on - strand, within IAI47_14725 at 3107.692 kb on + strand, within IAI47_14725 at 3107.693 kb on - strand, within IAI47_14725 at 3107.760 kb on - strand, within IAI47_14725 at 3107.784 kb on - strand, within IAI47_14725 at 3107.805 kb on + strand, within IAI47_14725 at 3107.805 kb on + strand, within IAI47_14725 at 3107.805 kb on + strand, within IAI47_14725 at 3107.805 kb on + strand, within IAI47_14725 at 3107.805 kb on + strand, within IAI47_14725 at 3107.806 kb on - strand, within IAI47_14725 at 3107.806 kb on - strand, within IAI47_14725 at 3107.809 kb on + strand, within IAI47_14725 at 3107.809 kb on + strand, within IAI47_14725 at 3107.809 kb on + strand, within IAI47_14725 at 3107.809 kb on + strand, within IAI47_14725 at 3107.809 kb on + strand, within IAI47_14725 at 3107.810 kb on - strand, within IAI47_14725 at 3107.810 kb on - strand, within IAI47_14725 at 3107.810 kb on - strand, within IAI47_14725 at 3107.837 kb on + strand, within IAI47_14725 at 3107.837 kb on + strand, within IAI47_14725 at 3107.837 kb on + strand, within IAI47_14725 at 3107.837 kb on + strand, within IAI47_14725 at 3107.837 kb on + strand, within IAI47_14725 at 3107.837 kb on + strand, within IAI47_14725 at 3107.837 kb on + strand, within IAI47_14725 at 3107.837 kb on + strand, within IAI47_14725 at 3107.837 kb on + strand, within IAI47_14725 at 3107.837 kb on + strand, within IAI47_14725 at 3107.837 kb on + strand, within IAI47_14725 at 3107.837 kb on + strand, within IAI47_14725 at 3107.838 kb on - strand, within IAI47_14725 at 3107.838 kb on - strand, within IAI47_14725 at 3107.838 kb on - strand, within IAI47_14725 at 3107.838 kb on - strand, within IAI47_14725 at 3107.838 kb on - strand, within IAI47_14725 at 3107.838 kb on - strand, within IAI47_14725 at 3107.855 kb on + strand, within IAI47_14725 at 3107.856 kb on - strand, within IAI47_14725 at 3107.905 kb on - strand, within IAI47_14725 at 3107.931 kb on - strand, within IAI47_14725 at 3107.931 kb on - strand, within IAI47_14725 at 3107.938 kb on + strand, within IAI47_14725 at 3107.938 kb on + strand, within IAI47_14725 at 3107.939 kb on - strand, within IAI47_14725 at 3107.973 kb on - strand, within IAI47_14725 at 3108.002 kb on + strand, within IAI47_14725 at 3108.002 kb on + strand, within IAI47_14725 at 3108.002 kb on + strand, within IAI47_14725 at 3108.002 kb on + strand, within IAI47_14725 at 3108.002 kb on + strand, within IAI47_14725 at 3108.002 kb on + strand, within IAI47_14725 at 3108.003 kb on - strand, within IAI47_14725 at 3108.003 kb on - strand, within IAI47_14725 at 3108.003 kb on - strand, within IAI47_14725 at 3108.003 kb on - strand, within IAI47_14725 at 3108.003 kb on - strand, within IAI47_14725 at 3108.003 kb on - strand, within IAI47_14725 at 3108.101 kb on + strand at 3108.101 kb on + strand at 3108.102 kb on - strand at 3108.102 kb on - strand at 3108.102 kb on - strand at 3108.123 kb on - strand at 3108.168 kb on + strand at 3108.169 kb on - strand at 3108.169 kb on - strand at 3108.213 kb on + strand at 3108.213 kb on + strand at 3108.213 kb on + strand at 3108.213 kb on + strand at 3108.213 kb on + strand at 3108.214 kb on - strand at 3108.214 kb on - strand at 3108.214 kb on - strand at 3108.214 kb on - strand at 3108.214 kb on - strand at 3108.214 kb on - strand at 3108.236 kb on + strand at 3108.236 kb on + strand at 3108.237 kb on - strand at 3108.237 kb on - strand at 3108.237 kb on - strand at 3108.237 kb on - strand at 3108.237 kb on - strand at 3108.244 kb on - strand at 3108.296 kb on + strand at 3108.296 kb on + strand at 3108.296 kb on + strand at 3108.296 kb on + strand at 3108.296 kb on + strand at 3108.296 kb on + strand at 3108.296 kb on + strand at 3108.297 kb on - strand at 3108.297 kb on - strand at 3108.297 kb on - strand at 3108.297 kb on - strand at 3108.298 kb on + strand at 3108.298 kb on + strand at 3108.298 kb on + strand at 3108.298 kb on + strand at 3108.298 kb on + strand at 3108.298 kb on + strand at 3108.298 kb on + strand at 3108.298 kb on + strand at 3108.298 kb on + strand at 3108.298 kb on + strand at 3108.299 kb on - strand at 3108.299 kb on - strand at 3108.345 kb on + strand at 3108.371 kb on + strand at 3108.371 kb on + strand at 3108.371 kb on + strand at 3108.391 kb on + strand at 3108.420 kb on + strand at 3108.420 kb on + strand at 3108.621 kb on + strand, within IAI47_14730 at 3108.621 kb on + strand, within IAI47_14730 at 3109.560 kb on + strand at 3109.560 kb on + strand at 3109.569 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 3,107,470 - IAI47_14725 0.50 +1.2 3,107,470 - IAI47_14725 0.50 +0.3 3,107,470 - IAI47_14725 0.50 +0.1 3,107,479 - IAI47_14725 0.51 +0.8 3,107,512 + IAI47_14725 0.53 +0.4 3,107,512 + IAI47_14725 0.53 -0.8 3,107,512 + IAI47_14725 0.53 -0.9 3,107,512 + IAI47_14725 0.53 +1.6 3,107,513 - IAI47_14725 0.53 +0.6 3,107,513 - IAI47_14725 0.53 -0.3 3,107,513 - IAI47_14725 0.53 -0.4 3,107,513 - IAI47_14725 0.53 -2.3 3,107,561 + IAI47_14725 0.57 -0.5 3,107,572 + IAI47_14725 0.57 -0.5 3,107,572 + IAI47_14725 0.57 -1.7 3,107,603 + IAI47_14725 0.60 +1.0 3,107,603 + IAI47_14725 0.60 +1.9 3,107,603 + IAI47_14725 0.60 -0.3 3,107,603 + IAI47_14725 0.60 +0.7 3,107,603 + IAI47_14725 0.60 -0.1 3,107,604 - IAI47_14725 0.60 +1.8 3,107,604 - IAI47_14725 0.60 +0.1 3,107,604 - IAI47_14725 0.60 +1.0 3,107,604 - IAI47_14725 0.60 -0.1 3,107,605 + IAI47_14725 0.60 -0.6 3,107,605 + IAI47_14725 0.60 -2.0 3,107,605 + IAI47_14725 0.60 -0.1 3,107,605 + IAI47_14725 0.60 +1.9 3,107,605 + IAI47_14725 0.60 +1.6 3,107,605 + IAI47_14725 0.60 -0.4 3,107,605 + IAI47_14725 0.60 -1.8 3,107,605 + IAI47_14725 0.60 -0.3 3,107,606 - IAI47_14725 0.60 -1.7 3,107,606 - IAI47_14725 0.60 -1.9 3,107,606 - IAI47_14725 0.60 +1.2 3,107,606 - IAI47_14725 0.60 +0.2 3,107,606 - IAI47_14725 0.60 +0.3 3,107,606 - IAI47_14725 0.60 +0.8 3,107,692 + IAI47_14725 0.66 +1.6 3,107,693 - IAI47_14725 0.66 +0.8 3,107,760 - IAI47_14725 0.70 +0.7 3,107,784 - IAI47_14725 0.72 -1.6 3,107,805 + IAI47_14725 0.73 +1.6 3,107,805 + IAI47_14725 0.73 -0.4 3,107,805 + IAI47_14725 0.73 +0.6 3,107,805 + IAI47_14725 0.73 +1.6 3,107,805 + IAI47_14725 0.73 +0.4 3,107,806 - IAI47_14725 0.74 -0.4 3,107,806 - IAI47_14725 0.74 -0.6 3,107,809 + IAI47_14725 0.74 -1.5 3,107,809 + IAI47_14725 0.74 +1.4 3,107,809 + IAI47_14725 0.74 -1.9 3,107,809 + IAI47_14725 0.74 +1.7 3,107,809 + IAI47_14725 0.74 -0.1 3,107,810 - IAI47_14725 0.74 +0.2 3,107,810 - IAI47_14725 0.74 +1.9 3,107,810 - IAI47_14725 0.74 -0.9 3,107,837 + IAI47_14725 0.76 +0.8 3,107,837 + IAI47_14725 0.76 -0.5 3,107,837 + IAI47_14725 0.76 +2.3 3,107,837 + IAI47_14725 0.76 +2.0 3,107,837 + IAI47_14725 0.76 -1.3 3,107,837 + IAI47_14725 0.76 -1.3 3,107,837 + IAI47_14725 0.76 -0.7 3,107,837 + IAI47_14725 0.76 -0.7 3,107,837 + IAI47_14725 0.76 -0.3 3,107,837 + IAI47_14725 0.76 -0.7 3,107,837 + IAI47_14725 0.76 -2.1 3,107,837 + IAI47_14725 0.76 -0.6 3,107,838 - IAI47_14725 0.76 +2.3 3,107,838 - IAI47_14725 0.76 +0.6 3,107,838 - IAI47_14725 0.76 -0.0 3,107,838 - IAI47_14725 0.76 -2.0 3,107,838 - IAI47_14725 0.76 -0.6 3,107,838 - IAI47_14725 0.76 +0.3 3,107,855 + IAI47_14725 0.77 -1.4 3,107,856 - IAI47_14725 0.77 -1.6 3,107,905 - IAI47_14725 0.80 +1.3 3,107,931 - IAI47_14725 0.82 +0.8 3,107,931 - IAI47_14725 0.82 +2.0 3,107,938 + IAI47_14725 0.83 -1.1 3,107,938 + IAI47_14725 0.83 -1.5 3,107,939 - IAI47_14725 0.83 -0.4 3,107,973 - IAI47_14725 0.85 +0.3 3,108,002 + IAI47_14725 0.87 +1.3 3,108,002 + IAI47_14725 0.87 +1.3 3,108,002 + IAI47_14725 0.87 -0.7 3,108,002 + IAI47_14725 0.87 +0.5 3,108,002 + IAI47_14725 0.87 +0.8 3,108,002 + IAI47_14725 0.87 -1.2 3,108,003 - IAI47_14725 0.87 +2.1 3,108,003 - IAI47_14725 0.87 -0.1 3,108,003 - IAI47_14725 0.87 +0.2 3,108,003 - IAI47_14725 0.87 -1.5 3,108,003 - IAI47_14725 0.87 +1.0 3,108,003 - IAI47_14725 0.87 +1.2 3,108,101 + -0.2 3,108,101 + -1.8 3,108,102 - -2.4 3,108,102 - -0.3 3,108,102 - +0.7 3,108,123 - -0.5 3,108,168 + -1.8 3,108,169 - -0.5 3,108,169 - +0.9 3,108,213 + -2.5 3,108,213 + -0.7 3,108,213 + -0.6 3,108,213 + -0.4 3,108,213 + +0.8 3,108,214 - -0.5 3,108,214 - -2.4 3,108,214 - +0.4 3,108,214 - -2.9 3,108,214 - -2.1 3,108,214 - +0.1 3,108,236 + +0.7 3,108,236 + +0.5 3,108,237 - +0.4 3,108,237 - +1.8 3,108,237 - +1.2 3,108,237 - -0.6 3,108,237 - -0.2 3,108,244 - -0.8 3,108,296 + +1.6 3,108,296 + +1.1 3,108,296 + +0.2 3,108,296 + +2.6 3,108,296 + +0.1 3,108,296 + -2.4 3,108,296 + +0.5 3,108,297 - -2.3 3,108,297 - +0.5 3,108,297 - -0.1 3,108,297 - +1.1 3,108,298 + -1.8 3,108,298 + +0.7 3,108,298 + -0.2 3,108,298 + +1.3 3,108,298 + +0.6 3,108,298 + -0.8 3,108,298 + +0.8 3,108,298 + +0.2 3,108,298 + -1.8 3,108,298 + +0.2 3,108,299 - +0.4 3,108,299 - +1.0 3,108,345 + +0.6 3,108,371 + -0.3 3,108,371 + -1.7 3,108,371 + -1.0 3,108,391 + +0.1 3,108,420 + -1.1 3,108,420 + +1.8 3,108,621 + IAI47_14730 0.82 +3.3 3,108,621 + IAI47_14730 0.82 -1.0 3,109,560 + -0.8 3,109,560 + +0.5 3,109,569 + +1.0
Or see this region's nucleotide sequence