Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_01775 and IAI47_01780 are separated by 35 nucleotides IAI47_01780 and IAI47_01785 are separated by 58 nucleotides IAI47_01785 and IAI47_01790 are separated by 34 nucleotides
IAI47_01775: IAI47_01775 - 4-alpha-glucanotransferase, at 388,614 to 390,695
_01775
IAI47_01780: IAI47_01780 - Fe-S biogenesis protein NfuA, at 390,731 to 391,306
_01780
IAI47_01785: IAI47_01785 - DNA utilization protein GntX, at 391,365 to 392,045
_01785
IAI47_01790: IAI47_01790 - pimeloyl-ACP methyl ester esterase BioH, at 392,080 to 392,856
_01790
Position (kb) 390
391
392 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 389.749 kb on + strand, within IAI47_01775 at 389.749 kb on + strand, within IAI47_01775 at 389.749 kb on + strand, within IAI47_01775 at 389.750 kb on - strand, within IAI47_01775 at 389.750 kb on - strand, within IAI47_01775 at 389.791 kb on - strand, within IAI47_01775 at 389.799 kb on - strand, within IAI47_01775 at 389.896 kb on - strand, within IAI47_01775 at 389.952 kb on - strand, within IAI47_01775 at 389.971 kb on + strand, within IAI47_01775 at 389.971 kb on + strand, within IAI47_01775 at 389.972 kb on - strand, within IAI47_01775 at 390.011 kb on + strand, within IAI47_01775 at 390.012 kb on - strand, within IAI47_01775 at 390.021 kb on + strand, within IAI47_01775 at 390.021 kb on + strand, within IAI47_01775 at 390.021 kb on + strand, within IAI47_01775 at 390.021 kb on + strand, within IAI47_01775 at 390.021 kb on + strand, within IAI47_01775 at 390.022 kb on - strand, within IAI47_01775 at 390.022 kb on - strand, within IAI47_01775 at 390.022 kb on - strand, within IAI47_01775 at 390.022 kb on - strand, within IAI47_01775 at 390.129 kb on - strand, within IAI47_01775 at 390.220 kb on - strand, within IAI47_01775 at 390.220 kb on - strand, within IAI47_01775 at 390.220 kb on - strand, within IAI47_01775 at 390.233 kb on + strand, within IAI47_01775 at 390.233 kb on + strand, within IAI47_01775 at 390.234 kb on - strand, within IAI47_01775 at 390.234 kb on - strand, within IAI47_01775 at 390.248 kb on + strand, within IAI47_01775 at 390.249 kb on - strand, within IAI47_01775 at 390.409 kb on + strand, within IAI47_01775 at 390.536 kb on + strand at 390.536 kb on + strand at 390.568 kb on + strand at 390.568 kb on + strand at 390.569 kb on - strand at 390.569 kb on - strand at 390.569 kb on - strand at 390.576 kb on + strand at 390.576 kb on + strand at 390.581 kb on + strand at 390.581 kb on + strand at 390.582 kb on - strand at 390.582 kb on - strand at 390.582 kb on - strand at 390.582 kb on - strand at 390.641 kb on + strand at 390.641 kb on + strand at 390.641 kb on + strand at 390.642 kb on - strand at 390.642 kb on - strand at 390.642 kb on - strand at 390.693 kb on + strand at 390.726 kb on + strand at 390.726 kb on + strand at 390.726 kb on + strand at 390.726 kb on + strand at 390.726 kb on + strand at 390.726 kb on + strand at 390.726 kb on + strand at 390.727 kb on - strand at 390.727 kb on - strand at 390.727 kb on - strand at 390.727 kb on - strand at 390.735 kb on + strand at 390.735 kb on + strand at 390.736 kb on - strand at 390.888 kb on + strand, within IAI47_01780 at 391.093 kb on - strand, within IAI47_01780 at 391.116 kb on + strand, within IAI47_01780 at 391.116 kb on + strand, within IAI47_01780 at 391.117 kb on - strand, within IAI47_01780 at 391.117 kb on - strand, within IAI47_01780 at 391.179 kb on + strand, within IAI47_01780 at 391.179 kb on + strand, within IAI47_01780 at 391.180 kb on - strand, within IAI47_01780 at 391.329 kb on - strand at 391.334 kb on - strand at 391.334 kb on - strand at 391.342 kb on - strand at 391.344 kb on - strand at 391.347 kb on - strand at 391.347 kb on - strand at 391.347 kb on - strand at 391.347 kb on - strand at 391.347 kb on - strand at 391.366 kb on + strand at 391.367 kb on - strand at 391.367 kb on - strand at 391.367 kb on - strand at 391.374 kb on - strand at 391.374 kb on - strand at 391.374 kb on - strand at 391.374 kb on - strand at 391.374 kb on - strand at 391.420 kb on - strand at 391.420 kb on - strand at 391.440 kb on + strand, within IAI47_01785 at 391.440 kb on + strand, within IAI47_01785 at 391.440 kb on + strand, within IAI47_01785 at 391.440 kb on + strand, within IAI47_01785 at 391.441 kb on - strand, within IAI47_01785 at 391.441 kb on - strand, within IAI47_01785 at 391.441 kb on - strand, within IAI47_01785 at 391.441 kb on - strand, within IAI47_01785 at 391.441 kb on - strand, within IAI47_01785 at 391.441 kb on - strand, within IAI47_01785 at 391.445 kb on + strand, within IAI47_01785 at 391.457 kb on + strand, within IAI47_01785 at 391.457 kb on + strand, within IAI47_01785 at 391.457 kb on + strand, within IAI47_01785 at 391.457 kb on + strand, within IAI47_01785 at 391.457 kb on + strand, within IAI47_01785 at 391.457 kb on + strand, within IAI47_01785 at 391.457 kb on + strand, within IAI47_01785 at 391.458 kb on - strand, within IAI47_01785 at 391.458 kb on - strand, within IAI47_01785 at 391.458 kb on - strand, within IAI47_01785 at 391.481 kb on + strand, within IAI47_01785 at 391.524 kb on + strand, within IAI47_01785 at 391.524 kb on + strand, within IAI47_01785 at 391.524 kb on + strand, within IAI47_01785 at 391.524 kb on + strand, within IAI47_01785 at 391.525 kb on - strand, within IAI47_01785 at 391.525 kb on - strand, within IAI47_01785 at 391.530 kb on - strand, within IAI47_01785 at 391.563 kb on + strand, within IAI47_01785 at 391.563 kb on + strand, within IAI47_01785 at 391.563 kb on + strand, within IAI47_01785 at 391.564 kb on - strand, within IAI47_01785 at 391.564 kb on - strand, within IAI47_01785 at 391.564 kb on - strand, within IAI47_01785 at 391.739 kb on + strand, within IAI47_01785 at 391.739 kb on + strand, within IAI47_01785 at 391.740 kb on - strand, within IAI47_01785 at 391.740 kb on - strand, within IAI47_01785 at 391.788 kb on + strand, within IAI47_01785 at 391.788 kb on + strand, within IAI47_01785 at 391.789 kb on - strand, within IAI47_01785 at 391.812 kb on - strand, within IAI47_01785 at 391.837 kb on + strand, within IAI47_01785 at 391.837 kb on + strand, within IAI47_01785 at 391.837 kb on + strand, within IAI47_01785 at 391.837 kb on + strand, within IAI47_01785 at 391.837 kb on + strand, within IAI47_01785 at 391.838 kb on - strand, within IAI47_01785 at 391.838 kb on - strand, within IAI47_01785 at 391.838 kb on - strand, within IAI47_01785 at 391.838 kb on - strand, within IAI47_01785 at 391.838 kb on - strand, within IAI47_01785 at 391.838 kb on - strand, within IAI47_01785 at 391.838 kb on - strand, within IAI47_01785 at 391.878 kb on - strand, within IAI47_01785 at 391.957 kb on - strand, within IAI47_01785 at 391.971 kb on + strand, within IAI47_01785 at 391.971 kb on + strand, within IAI47_01785 at 391.971 kb on + strand, within IAI47_01785 at 391.971 kb on + strand, within IAI47_01785 at 391.972 kb on - strand, within IAI47_01785 at 391.972 kb on - strand, within IAI47_01785 at 391.972 kb on - strand, within IAI47_01785 at 391.972 kb on - strand, within IAI47_01785 at 392.040 kb on + strand at 392.040 kb on + strand at 392.040 kb on + strand at 392.041 kb on - strand at 392.041 kb on - strand at 392.041 kb on - strand at 392.041 kb on - strand at 392.045 kb on + strand at 392.045 kb on + strand at 392.045 kb on + strand at 392.046 kb on - strand at 392.057 kb on + strand at 392.071 kb on + strand at 392.072 kb on - strand at 392.072 kb on - strand at 392.073 kb on + strand at 392.073 kb on + strand at 392.074 kb on + strand at 392.074 kb on + strand at 392.074 kb on + strand at 392.074 kb on + strand at 392.074 kb on + strand at 392.074 kb on + strand at 392.074 kb on + strand at 392.074 kb on + strand at 392.074 kb on + strand at 392.075 kb on - strand at 392.075 kb on - strand at 392.075 kb on - strand at 392.075 kb on - strand at 392.075 kb on - strand at 392.075 kb on - strand at 392.075 kb on - strand at 392.075 kb on - strand at 392.175 kb on + strand, within IAI47_01790 at 392.175 kb on + strand, within IAI47_01790 at 392.175 kb on + strand, within IAI47_01790 at 392.197 kb on + strand, within IAI47_01790 at 392.197 kb on + strand, within IAI47_01790 at 392.198 kb on - strand, within IAI47_01790 at 392.198 kb on - strand, within IAI47_01790 at 392.282 kb on + strand, within IAI47_01790 at 392.282 kb on + strand, within IAI47_01790 at 392.282 kb on + strand, within IAI47_01790 at 392.283 kb on - strand, within IAI47_01790 at 392.283 kb on - strand, within IAI47_01790 at 392.283 kb on - strand, within IAI47_01790 at 392.283 kb on - strand, within IAI47_01790
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 389,749 + IAI47_01775 0.55 +1.2 389,749 + IAI47_01775 0.55 -2.3 389,749 + IAI47_01775 0.55 +0.7 389,750 - IAI47_01775 0.55 -2.7 389,750 - IAI47_01775 0.55 +0.7 389,791 - IAI47_01775 0.57 +0.8 389,799 - IAI47_01775 0.57 +0.3 389,896 - IAI47_01775 0.62 -2.7 389,952 - IAI47_01775 0.64 -0.7 389,971 + IAI47_01775 0.65 -0.3 389,971 + IAI47_01775 0.65 -0.1 389,972 - IAI47_01775 0.65 +0.5 390,011 + IAI47_01775 0.67 +2.7 390,012 - IAI47_01775 0.67 -1.6 390,021 + IAI47_01775 0.68 -0.9 390,021 + IAI47_01775 0.68 -1.4 390,021 + IAI47_01775 0.68 +0.4 390,021 + IAI47_01775 0.68 -1.0 390,021 + IAI47_01775 0.68 +1.0 390,022 - IAI47_01775 0.68 +1.6 390,022 - IAI47_01775 0.68 -0.1 390,022 - IAI47_01775 0.68 -1.6 390,022 - IAI47_01775 0.68 -0.0 390,129 - IAI47_01775 0.73 -0.6 390,220 - IAI47_01775 0.77 -0.7 390,220 - IAI47_01775 0.77 +0.6 390,220 - IAI47_01775 0.77 -0.2 390,233 + IAI47_01775 0.78 +1.6 390,233 + IAI47_01775 0.78 +0.1 390,234 - IAI47_01775 0.78 +0.8 390,234 - IAI47_01775 0.78 -0.4 390,248 + IAI47_01775 0.78 +1.0 390,249 - IAI47_01775 0.79 -0.3 390,409 + IAI47_01775 0.86 -2.1 390,536 + +0.4 390,536 + +0.5 390,568 + -0.6 390,568 + +0.6 390,569 - -0.2 390,569 - -0.2 390,569 - -0.9 390,576 + +0.8 390,576 + +0.5 390,581 + -0.1 390,581 + -0.0 390,582 - +0.5 390,582 - -0.7 390,582 - -0.6 390,582 - +1.3 390,641 + +1.0 390,641 + +1.2 390,641 + +1.4 390,642 - +0.4 390,642 - -0.2 390,642 - -2.5 390,693 + -1.8 390,726 + +1.2 390,726 + +1.5 390,726 + +0.9 390,726 + +0.1 390,726 + +0.9 390,726 + +1.4 390,726 + +2.8 390,727 - +0.5 390,727 - -1.3 390,727 - +1.1 390,727 - +0.7 390,735 + -1.4 390,735 + +1.1 390,736 - +0.5 390,888 + IAI47_01780 0.27 +0.7 391,093 - IAI47_01780 0.63 -1.2 391,116 + IAI47_01780 0.67 +2.7 391,116 + IAI47_01780 0.67 -1.1 391,117 - IAI47_01780 0.67 -0.6 391,117 - IAI47_01780 0.67 +0.4 391,179 + IAI47_01780 0.78 +0.1 391,179 + IAI47_01780 0.78 +1.7 391,180 - IAI47_01780 0.78 -1.3 391,329 - -0.2 391,334 - +0.7 391,334 - -0.8 391,342 - +0.7 391,344 - -0.5 391,347 - -0.0 391,347 - -0.1 391,347 - -1.6 391,347 - -0.6 391,347 - +3.2 391,366 + -0.4 391,367 - +2.0 391,367 - +0.2 391,367 - -0.3 391,374 - +0.7 391,374 - -1.1 391,374 - +0.5 391,374 - +1.6 391,374 - -0.9 391,420 - +1.1 391,420 - -1.8 391,440 + IAI47_01785 0.11 +0.5 391,440 + IAI47_01785 0.11 -1.1 391,440 + IAI47_01785 0.11 -0.7 391,440 + IAI47_01785 0.11 -1.1 391,441 - IAI47_01785 0.11 +0.1 391,441 - IAI47_01785 0.11 -0.1 391,441 - IAI47_01785 0.11 -0.8 391,441 - IAI47_01785 0.11 +0.7 391,441 - IAI47_01785 0.11 +0.1 391,441 - IAI47_01785 0.11 +2.0 391,445 + IAI47_01785 0.12 +0.1 391,457 + IAI47_01785 0.14 +1.0 391,457 + IAI47_01785 0.14 -0.9 391,457 + IAI47_01785 0.14 -1.4 391,457 + IAI47_01785 0.14 +0.4 391,457 + IAI47_01785 0.14 -1.9 391,457 + IAI47_01785 0.14 +0.8 391,457 + IAI47_01785 0.14 -1.6 391,458 - IAI47_01785 0.14 +0.5 391,458 - IAI47_01785 0.14 -0.4 391,458 - IAI47_01785 0.14 -1.3 391,481 + IAI47_01785 0.17 +2.0 391,524 + IAI47_01785 0.23 -0.5 391,524 + IAI47_01785 0.23 -1.0 391,524 + IAI47_01785 0.23 +1.4 391,524 + IAI47_01785 0.23 -1.2 391,525 - IAI47_01785 0.23 +2.0 391,525 - IAI47_01785 0.23 -1.2 391,530 - IAI47_01785 0.24 +1.1 391,563 + IAI47_01785 0.29 -2.3 391,563 + IAI47_01785 0.29 -0.5 391,563 + IAI47_01785 0.29 +0.1 391,564 - IAI47_01785 0.29 -1.4 391,564 - IAI47_01785 0.29 -0.0 391,564 - IAI47_01785 0.29 +1.6 391,739 + IAI47_01785 0.55 -0.3 391,739 + IAI47_01785 0.55 -0.8 391,740 - IAI47_01785 0.55 -0.3 391,740 - IAI47_01785 0.55 +1.1 391,788 + IAI47_01785 0.62 +0.6 391,788 + IAI47_01785 0.62 -2.9 391,789 - IAI47_01785 0.62 -1.4 391,812 - IAI47_01785 0.66 +0.2 391,837 + IAI47_01785 0.69 -2.3 391,837 + IAI47_01785 0.69 +0.5 391,837 + IAI47_01785 0.69 -0.4 391,837 + IAI47_01785 0.69 -2.6 391,837 + IAI47_01785 0.69 -0.8 391,838 - IAI47_01785 0.69 +0.1 391,838 - IAI47_01785 0.69 -0.7 391,838 - IAI47_01785 0.69 -0.3 391,838 - IAI47_01785 0.69 -0.2 391,838 - IAI47_01785 0.69 +1.0 391,838 - IAI47_01785 0.69 -1.9 391,838 - IAI47_01785 0.69 +0.2 391,878 - IAI47_01785 0.75 +0.8 391,957 - IAI47_01785 0.87 -0.0 391,971 + IAI47_01785 0.89 -0.3 391,971 + IAI47_01785 0.89 -0.0 391,971 + IAI47_01785 0.89 -0.1 391,971 + IAI47_01785 0.89 +0.6 391,972 - IAI47_01785 0.89 +0.0 391,972 - IAI47_01785 0.89 +0.1 391,972 - IAI47_01785 0.89 -1.7 391,972 - IAI47_01785 0.89 -1.1 392,040 + -1.3 392,040 + +1.6 392,040 + -0.6 392,041 - -1.1 392,041 - +0.8 392,041 - -2.4 392,041 - +2.3 392,045 + -0.4 392,045 + -1.7 392,045 + -1.8 392,046 - -0.2 392,057 + -0.6 392,071 + +2.1 392,072 - -0.6 392,072 - +0.5 392,073 + -0.0 392,073 + +0.0 392,074 + +2.3 392,074 + +1.2 392,074 + +0.1 392,074 + -3.0 392,074 + -0.7 392,074 + -0.1 392,074 + -0.3 392,074 + +1.1 392,074 + +3.2 392,075 - -1.3 392,075 - -0.2 392,075 - -1.6 392,075 - +1.9 392,075 - -0.3 392,075 - +1.0 392,075 - +1.0 392,075 - +1.6 392,175 + IAI47_01790 0.12 +0.4 392,175 + IAI47_01790 0.12 +1.4 392,175 + IAI47_01790 0.12 +0.9 392,197 + IAI47_01790 0.15 +0.4 392,197 + IAI47_01790 0.15 +1.1 392,198 - IAI47_01790 0.15 +0.7 392,198 - IAI47_01790 0.15 -1.0 392,282 + IAI47_01790 0.26 +1.4 392,282 + IAI47_01790 0.26 +0.6 392,282 + IAI47_01790 0.26 +2.8 392,283 - IAI47_01790 0.26 -0.5 392,283 - IAI47_01790 0.26 -0.5 392,283 - IAI47_01790 0.26 +1.1 392,283 - IAI47_01790 0.26 +1.0
Or see this region's nucleotide sequence