Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_19055 and IAI47_19060 overlap by 4 nucleotides IAI47_19060 and IAI47_19065 are separated by 2 nucleotides IAI47_19065 and IAI47_19070 overlap by 1 nucleotides
IAI47_19055: IAI47_19055 - metal ABC transporter substrate-binding protein, at 39,944 to 40,858
_19055
IAI47_19060: IAI47_19060 - manganese/iron ABC transporter ATP-binding protein, at 40,855 to 41,670
_19060
IAI47_19065: IAI47_19065 - iron/manganese ABC transporter permease subunit SitC, at 41,673 to 42,533
_19065
IAI47_19070: IAI47_19070 - metal ABC transporter permease, at 42,533 to 43,384
_19070
Position (kb) 41
42
43 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 40.728 kb on - strand, within IAI47_19055 at 40.728 kb on - strand, within IAI47_19055 at 40.728 kb on - strand, within IAI47_19055 at 40.730 kb on + strand, within IAI47_19055 at 40.730 kb on + strand, within IAI47_19055 at 40.730 kb on + strand, within IAI47_19055 at 40.730 kb on + strand, within IAI47_19055 at 40.731 kb on - strand, within IAI47_19055 at 40.745 kb on + strand, within IAI47_19055 at 40.745 kb on + strand, within IAI47_19055 at 40.745 kb on + strand, within IAI47_19055 at 40.745 kb on + strand, within IAI47_19055 at 40.745 kb on + strand, within IAI47_19055 at 40.746 kb on - strand, within IAI47_19055 at 40.746 kb on - strand, within IAI47_19055 at 40.787 kb on + strand at 40.788 kb on - strand at 40.788 kb on - strand at 40.788 kb on - strand at 40.788 kb on - strand at 40.788 kb on - strand at 40.801 kb on + strand at 40.801 kb on + strand at 40.801 kb on + strand at 40.802 kb on - strand at 40.834 kb on + strand at 40.835 kb on - strand at 40.894 kb on + strand at 40.894 kb on + strand at 40.894 kb on + strand at 40.895 kb on - strand at 40.895 kb on - strand at 40.895 kb on - strand at 40.900 kb on - strand at 40.964 kb on + strand, within IAI47_19060 at 40.964 kb on + strand, within IAI47_19060 at 40.965 kb on - strand, within IAI47_19060 at 40.984 kb on - strand, within IAI47_19060 at 40.999 kb on - strand, within IAI47_19060 at 41.052 kb on + strand, within IAI47_19060 at 41.087 kb on + strand, within IAI47_19060 at 41.087 kb on + strand, within IAI47_19060 at 41.087 kb on + strand, within IAI47_19060 at 41.087 kb on + strand, within IAI47_19060 at 41.087 kb on + strand, within IAI47_19060 at 41.088 kb on - strand, within IAI47_19060 at 41.092 kb on + strand, within IAI47_19060 at 41.092 kb on + strand, within IAI47_19060 at 41.092 kb on + strand, within IAI47_19060 at 41.093 kb on - strand, within IAI47_19060 at 41.093 kb on - strand, within IAI47_19060 at 41.164 kb on + strand, within IAI47_19060 at 41.172 kb on + strand, within IAI47_19060 at 41.172 kb on + strand, within IAI47_19060 at 41.173 kb on - strand, within IAI47_19060 at 41.173 kb on - strand, within IAI47_19060 at 41.173 kb on - strand, within IAI47_19060 at 41.173 kb on - strand, within IAI47_19060 at 41.173 kb on - strand, within IAI47_19060 at 41.184 kb on - strand, within IAI47_19060 at 41.241 kb on + strand, within IAI47_19060 at 41.241 kb on + strand, within IAI47_19060 at 41.242 kb on - strand, within IAI47_19060 at 41.251 kb on + strand, within IAI47_19060 at 41.251 kb on + strand, within IAI47_19060 at 41.252 kb on - strand, within IAI47_19060 at 41.252 kb on - strand, within IAI47_19060 at 41.300 kb on + strand, within IAI47_19060 at 41.300 kb on + strand, within IAI47_19060 at 41.300 kb on + strand, within IAI47_19060 at 41.300 kb on + strand, within IAI47_19060 at 41.301 kb on - strand, within IAI47_19060 at 41.448 kb on + strand, within IAI47_19060 at 41.448 kb on + strand, within IAI47_19060 at 41.448 kb on + strand, within IAI47_19060 at 41.448 kb on + strand, within IAI47_19060 at 41.448 kb on + strand, within IAI47_19060 at 41.448 kb on + strand, within IAI47_19060 at 41.449 kb on - strand, within IAI47_19060 at 41.449 kb on - strand, within IAI47_19060 at 41.449 kb on - strand, within IAI47_19060 at 41.449 kb on - strand, within IAI47_19060 at 41.480 kb on - strand, within IAI47_19060 at 41.494 kb on - strand, within IAI47_19060 at 41.535 kb on + strand, within IAI47_19060 at 41.535 kb on + strand, within IAI47_19060 at 41.570 kb on + strand, within IAI47_19060 at 41.571 kb on - strand, within IAI47_19060 at 41.571 kb on - strand, within IAI47_19060 at 41.683 kb on + strand at 41.683 kb on + strand at 41.706 kb on + strand at 41.706 kb on + strand at 41.706 kb on + strand at 41.706 kb on + strand at 41.706 kb on + strand at 41.706 kb on + strand at 41.706 kb on + strand at 41.706 kb on + strand at 41.706 kb on + strand at 41.707 kb on - strand at 41.707 kb on - strand at 41.707 kb on - strand at 41.707 kb on - strand at 41.707 kb on - strand at 41.707 kb on - strand at 41.707 kb on - strand at 41.707 kb on - strand at 41.736 kb on - strand at 41.736 kb on - strand at 41.769 kb on + strand, within IAI47_19065 at 41.770 kb on - strand, within IAI47_19065 at 41.770 kb on - strand, within IAI47_19065 at 41.844 kb on + strand, within IAI47_19065 at 41.844 kb on + strand, within IAI47_19065 at 41.844 kb on + strand, within IAI47_19065 at 41.844 kb on + strand, within IAI47_19065 at 41.844 kb on + strand, within IAI47_19065 at 41.844 kb on + strand, within IAI47_19065 at 41.845 kb on - strand, within IAI47_19065 at 41.845 kb on - strand, within IAI47_19065 at 41.845 kb on - strand, within IAI47_19065 at 41.845 kb on - strand, within IAI47_19065 at 41.845 kb on - strand, within IAI47_19065 at 41.845 kb on - strand, within IAI47_19065 at 41.845 kb on - strand, within IAI47_19065 at 41.845 kb on - strand, within IAI47_19065 at 41.918 kb on + strand, within IAI47_19065 at 41.918 kb on + strand, within IAI47_19065 at 41.919 kb on - strand, within IAI47_19065 at 41.919 kb on - strand, within IAI47_19065 at 41.993 kb on + strand, within IAI47_19065 at 41.993 kb on + strand, within IAI47_19065 at 41.993 kb on + strand, within IAI47_19065 at 41.994 kb on - strand, within IAI47_19065 at 41.994 kb on - strand, within IAI47_19065 at 42.048 kb on - strand, within IAI47_19065 at 42.112 kb on + strand, within IAI47_19065 at 42.112 kb on + strand, within IAI47_19065 at 42.112 kb on + strand, within IAI47_19065 at 42.112 kb on + strand, within IAI47_19065 at 42.112 kb on + strand, within IAI47_19065 at 42.112 kb on + strand, within IAI47_19065 at 42.113 kb on - strand, within IAI47_19065 at 42.113 kb on - strand, within IAI47_19065 at 42.113 kb on - strand, within IAI47_19065 at 42.113 kb on - strand, within IAI47_19065 at 42.122 kb on + strand, within IAI47_19065 at 42.123 kb on - strand, within IAI47_19065 at 42.123 kb on - strand, within IAI47_19065 at 42.212 kb on + strand, within IAI47_19065 at 42.283 kb on + strand, within IAI47_19065 at 42.284 kb on - strand, within IAI47_19065 at 42.284 kb on - strand, within IAI47_19065 at 42.390 kb on + strand, within IAI47_19065 at 42.390 kb on + strand, within IAI47_19065 at 42.390 kb on + strand, within IAI47_19065 at 42.390 kb on + strand, within IAI47_19065 at 42.391 kb on - strand, within IAI47_19065 at 42.391 kb on - strand, within IAI47_19065 at 42.393 kb on + strand, within IAI47_19065 at 42.394 kb on - strand, within IAI47_19065 at 42.394 kb on - strand, within IAI47_19065 at 42.397 kb on + strand, within IAI47_19065 at 42.397 kb on + strand, within IAI47_19065 at 42.397 kb on + strand, within IAI47_19065 at 42.397 kb on + strand, within IAI47_19065 at 42.397 kb on + strand, within IAI47_19065 at 42.398 kb on - strand, within IAI47_19065 at 42.398 kb on - strand, within IAI47_19065 at 42.398 kb on - strand, within IAI47_19065 at 42.398 kb on - strand, within IAI47_19065 at 42.398 kb on - strand, within IAI47_19065 at 42.398 kb on - strand, within IAI47_19065 at 42.579 kb on + strand at 42.580 kb on - strand at 42.580 kb on - strand at 42.598 kb on - strand at 42.624 kb on + strand, within IAI47_19070 at 42.624 kb on + strand, within IAI47_19070 at 42.625 kb on - strand, within IAI47_19070 at 42.802 kb on + strand, within IAI47_19070 at 42.802 kb on + strand, within IAI47_19070 at 42.802 kb on + strand, within IAI47_19070 at 42.803 kb on - strand, within IAI47_19070 at 42.854 kb on + strand, within IAI47_19070 at 42.854 kb on + strand, within IAI47_19070 at 42.854 kb on + strand, within IAI47_19070 at 42.855 kb on - strand, within IAI47_19070 at 42.855 kb on - strand, within IAI47_19070 at 42.855 kb on - strand, within IAI47_19070 at 42.855 kb on - strand, within IAI47_19070 at 42.855 kb on - strand, within IAI47_19070 at 42.857 kb on - strand, within IAI47_19070 at 42.857 kb on - strand, within IAI47_19070 at 42.916 kb on + strand, within IAI47_19070 at 42.928 kb on + strand, within IAI47_19070 at 42.963 kb on + strand, within IAI47_19070 at 42.963 kb on + strand, within IAI47_19070 at 42.964 kb on - strand, within IAI47_19070 at 43.140 kb on + strand, within IAI47_19070 at 43.140 kb on + strand, within IAI47_19070 at 43.141 kb on - strand, within IAI47_19070 at 43.192 kb on - strand, within IAI47_19070 at 43.238 kb on + strand, within IAI47_19070 at 43.238 kb on + strand, within IAI47_19070 at 43.354 kb on + strand at 43.354 kb on + strand at 43.354 kb on + strand at 43.354 kb on + strand at 43.355 kb on - strand at 43.355 kb on - strand at 43.355 kb on - strand at 43.382 kb on + strand at 43.382 kb on + strand at 43.382 kb on + strand at 43.383 kb on - strand at 43.409 kb on - strand at 43.409 kb on - strand at 43.409 kb on - strand at 43.451 kb on - strand at 43.472 kb on + strand at 43.472 kb on + strand at 43.473 kb on - strand at 43.480 kb on + strand at 43.480 kb on + strand at 43.481 kb on - strand at 43.493 kb on + strand at 43.522 kb on - strand at 43.522 kb on - strand at 43.522 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 40,728 - IAI47_19055 0.86 +0.2 40,728 - IAI47_19055 0.86 -0.2 40,728 - IAI47_19055 0.86 -0.3 40,730 + IAI47_19055 0.86 +0.2 40,730 + IAI47_19055 0.86 -0.9 40,730 + IAI47_19055 0.86 -3.4 40,730 + IAI47_19055 0.86 +0.5 40,731 - IAI47_19055 0.86 -0.6 40,745 + IAI47_19055 0.88 -1.7 40,745 + IAI47_19055 0.88 -2.7 40,745 + IAI47_19055 0.88 -1.6 40,745 + IAI47_19055 0.88 -1.0 40,745 + IAI47_19055 0.88 +1.6 40,746 - IAI47_19055 0.88 -1.4 40,746 - IAI47_19055 0.88 -1.1 40,787 + -0.8 40,788 - -0.7 40,788 - -0.7 40,788 - -0.2 40,788 - +1.5 40,788 - -2.8 40,801 + +1.5 40,801 + +0.4 40,801 + +0.9 40,802 - +1.0 40,834 + +0.3 40,835 - -1.6 40,894 + +0.5 40,894 + +0.8 40,894 + +0.8 40,895 - +0.8 40,895 - +2.1 40,895 - +1.4 40,900 - +0.1 40,964 + IAI47_19060 0.13 -0.5 40,964 + IAI47_19060 0.13 -1.2 40,965 - IAI47_19060 0.13 -0.6 40,984 - IAI47_19060 0.16 -0.7 40,999 - IAI47_19060 0.18 -1.7 41,052 + IAI47_19060 0.24 -0.4 41,087 + IAI47_19060 0.28 +0.3 41,087 + IAI47_19060 0.28 -1.9 41,087 + IAI47_19060 0.28 -0.6 41,087 + IAI47_19060 0.28 +0.8 41,087 + IAI47_19060 0.28 -0.4 41,088 - IAI47_19060 0.29 -0.5 41,092 + IAI47_19060 0.29 +0.1 41,092 + IAI47_19060 0.29 +0.2 41,092 + IAI47_19060 0.29 +0.8 41,093 - IAI47_19060 0.29 -0.6 41,093 - IAI47_19060 0.29 +0.9 41,164 + IAI47_19060 0.38 +1.4 41,172 + IAI47_19060 0.39 -1.3 41,172 + IAI47_19060 0.39 -0.2 41,173 - IAI47_19060 0.39 -0.8 41,173 - IAI47_19060 0.39 -1.5 41,173 - IAI47_19060 0.39 +0.0 41,173 - IAI47_19060 0.39 +2.4 41,173 - IAI47_19060 0.39 -1.6 41,184 - IAI47_19060 0.40 -0.1 41,241 + IAI47_19060 0.47 +0.2 41,241 + IAI47_19060 0.47 -0.7 41,242 - IAI47_19060 0.47 +0.8 41,251 + IAI47_19060 0.49 +2.9 41,251 + IAI47_19060 0.49 -0.4 41,252 - IAI47_19060 0.49 -0.2 41,252 - IAI47_19060 0.49 +1.5 41,300 + IAI47_19060 0.55 +0.4 41,300 + IAI47_19060 0.55 +0.4 41,300 + IAI47_19060 0.55 -0.3 41,300 + IAI47_19060 0.55 -0.2 41,301 - IAI47_19060 0.55 +1.1 41,448 + IAI47_19060 0.73 +2.1 41,448 + IAI47_19060 0.73 -1.1 41,448 + IAI47_19060 0.73 -1.0 41,448 + IAI47_19060 0.73 -1.2 41,448 + IAI47_19060 0.73 +1.7 41,448 + IAI47_19060 0.73 +2.4 41,449 - IAI47_19060 0.73 -1.2 41,449 - IAI47_19060 0.73 +0.1 41,449 - IAI47_19060 0.73 -0.2 41,449 - IAI47_19060 0.73 -1.6 41,480 - IAI47_19060 0.77 -0.1 41,494 - IAI47_19060 0.78 -1.6 41,535 + IAI47_19060 0.83 +0.4 41,535 + IAI47_19060 0.83 +0.4 41,570 + IAI47_19060 0.88 -2.0 41,571 - IAI47_19060 0.88 +0.7 41,571 - IAI47_19060 0.88 -0.2 41,683 + +3.4 41,683 + -0.1 41,706 + +2.0 41,706 + +0.4 41,706 + +1.3 41,706 + -0.3 41,706 + -0.5 41,706 + -1.2 41,706 + +1.7 41,706 + -1.9 41,706 + -0.3 41,707 - -0.6 41,707 - +0.4 41,707 - +0.0 41,707 - +0.5 41,707 - +0.3 41,707 - -0.2 41,707 - -2.1 41,707 - +0.5 41,736 - +0.0 41,736 - -1.1 41,769 + IAI47_19065 0.11 +0.7 41,770 - IAI47_19065 0.11 +2.5 41,770 - IAI47_19065 0.11 -0.3 41,844 + IAI47_19065 0.20 -0.8 41,844 + IAI47_19065 0.20 +0.4 41,844 + IAI47_19065 0.20 -1.0 41,844 + IAI47_19065 0.20 -1.2 41,844 + IAI47_19065 0.20 -1.2 41,844 + IAI47_19065 0.20 -1.5 41,845 - IAI47_19065 0.20 +0.1 41,845 - IAI47_19065 0.20 +0.2 41,845 - IAI47_19065 0.20 -0.9 41,845 - IAI47_19065 0.20 -2.5 41,845 - IAI47_19065 0.20 +0.0 41,845 - IAI47_19065 0.20 +0.4 41,845 - IAI47_19065 0.20 -1.2 41,845 - IAI47_19065 0.20 -1.0 41,918 + IAI47_19065 0.28 +0.4 41,918 + IAI47_19065 0.28 +1.0 41,919 - IAI47_19065 0.29 -0.2 41,919 - IAI47_19065 0.29 -0.5 41,993 + IAI47_19065 0.37 -1.1 41,993 + IAI47_19065 0.37 -1.6 41,993 + IAI47_19065 0.37 -0.5 41,994 - IAI47_19065 0.37 -0.5 41,994 - IAI47_19065 0.37 +2.7 42,048 - IAI47_19065 0.44 +1.4 42,112 + IAI47_19065 0.51 +0.7 42,112 + IAI47_19065 0.51 -2.3 42,112 + IAI47_19065 0.51 -1.3 42,112 + IAI47_19065 0.51 -1.1 42,112 + IAI47_19065 0.51 +1.1 42,112 + IAI47_19065 0.51 +0.3 42,113 - IAI47_19065 0.51 +0.7 42,113 - IAI47_19065 0.51 -0.5 42,113 - IAI47_19065 0.51 -0.5 42,113 - IAI47_19065 0.51 -1.1 42,122 + IAI47_19065 0.52 -1.5 42,123 - IAI47_19065 0.52 +0.4 42,123 - IAI47_19065 0.52 -0.8 42,212 + IAI47_19065 0.63 -0.5 42,283 + IAI47_19065 0.71 -1.2 42,284 - IAI47_19065 0.71 -1.6 42,284 - IAI47_19065 0.71 -0.2 42,390 + IAI47_19065 0.83 +1.4 42,390 + IAI47_19065 0.83 +0.2 42,390 + IAI47_19065 0.83 +1.2 42,390 + IAI47_19065 0.83 -0.3 42,391 - IAI47_19065 0.83 +2.0 42,391 - IAI47_19065 0.83 -0.2 42,393 + IAI47_19065 0.84 -1.6 42,394 - IAI47_19065 0.84 +1.4 42,394 - IAI47_19065 0.84 -0.8 42,397 + IAI47_19065 0.84 -0.2 42,397 + IAI47_19065 0.84 +0.0 42,397 + IAI47_19065 0.84 -2.5 42,397 + IAI47_19065 0.84 -2.3 42,397 + IAI47_19065 0.84 -0.1 42,398 - IAI47_19065 0.84 -0.6 42,398 - IAI47_19065 0.84 +0.2 42,398 - IAI47_19065 0.84 +0.4 42,398 - IAI47_19065 0.84 +0.4 42,398 - IAI47_19065 0.84 -1.3 42,398 - IAI47_19065 0.84 -0.5 42,579 + -0.2 42,580 - -0.4 42,580 - +2.1 42,598 - +0.7 42,624 + IAI47_19070 0.11 +0.4 42,624 + IAI47_19070 0.11 +0.7 42,625 - IAI47_19070 0.11 -0.2 42,802 + IAI47_19070 0.32 +2.7 42,802 + IAI47_19070 0.32 +2.1 42,802 + IAI47_19070 0.32 -1.0 42,803 - IAI47_19070 0.32 -0.7 42,854 + IAI47_19070 0.38 -0.8 42,854 + IAI47_19070 0.38 +1.0 42,854 + IAI47_19070 0.38 +1.9 42,855 - IAI47_19070 0.38 +0.5 42,855 - IAI47_19070 0.38 -1.3 42,855 - IAI47_19070 0.38 -2.3 42,855 - IAI47_19070 0.38 -0.2 42,855 - IAI47_19070 0.38 +0.9 42,857 - IAI47_19070 0.38 -0.4 42,857 - IAI47_19070 0.38 -0.1 42,916 + IAI47_19070 0.45 +0.5 42,928 + IAI47_19070 0.46 +1.2 42,963 + IAI47_19070 0.50 -0.7 42,963 + IAI47_19070 0.50 -1.6 42,964 - IAI47_19070 0.51 -1.4 43,140 + IAI47_19070 0.71 +1.6 43,140 + IAI47_19070 0.71 -0.8 43,141 - IAI47_19070 0.71 -1.6 43,192 - IAI47_19070 0.77 -0.9 43,238 + IAI47_19070 0.83 +0.4 43,238 + IAI47_19070 0.83 -0.5 43,354 + -0.3 43,354 + +0.0 43,354 + +0.8 43,354 + +2.4 43,355 - -1.1 43,355 - -1.5 43,355 - -2.0 43,382 + -0.2 43,382 + +0.9 43,382 + +1.1 43,383 - +1.9 43,409 - -2.0 43,409 - +0.9 43,409 - +0.8 43,451 - -1.6 43,472 + +0.8 43,472 + +0.8 43,473 - +0.4 43,480 + +0.8 43,480 + +0.2 43,481 - -0.1 43,493 + +2.0 43,522 - +0.0 43,522 - +1.8 43,522 - +0.4
Or see this region's nucleotide sequence