Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_04470 and IAI47_04475 are separated by 99 nucleotides IAI47_04475 and IAI47_04480 are separated by 139 nucleotides IAI47_04480 and IAI47_04485 are separated by 82 nucleotides
IAI47_04470: IAI47_04470 - CDP-diacylglycerol--serine O-phosphatidyltransferase, at 938,989 to 940,344
_04470
IAI47_04475: IAI47_04475 - bifunctional acetate--CoA ligase family protein/GNAT family N-acetyltransferase, at 940,444 to 943,119
_04475
IAI47_04480: IAI47_04480 - DTW domain-containing protein, at 943,259 to 943,936
_04480
IAI47_04485: IAI47_04485 - thioredoxin TrxC, at 944,019 to 944,438
_04485
Position (kb) 940
941
942
943
944 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 940.412 kb on - strand at 940.456 kb on - strand at 940.456 kb on - strand at 940.503 kb on + strand at 940.504 kb on - strand at 940.536 kb on + strand at 940.537 kb on - strand at 940.537 kb on - strand at 940.537 kb on - strand at 940.554 kb on + strand at 940.554 kb on + strand at 940.555 kb on - strand at 940.585 kb on - strand at 940.620 kb on - strand at 940.620 kb on - strand at 940.708 kb on - strand at 940.708 kb on - strand at 940.723 kb on - strand, within IAI47_04475 at 940.733 kb on - strand, within IAI47_04475 at 940.796 kb on + strand, within IAI47_04475 at 940.799 kb on + strand, within IAI47_04475 at 940.812 kb on + strand, within IAI47_04475 at 940.812 kb on + strand, within IAI47_04475 at 940.813 kb on - strand, within IAI47_04475 at 940.856 kb on + strand, within IAI47_04475 at 940.862 kb on + strand, within IAI47_04475 at 940.863 kb on - strand, within IAI47_04475 at 940.865 kb on + strand, within IAI47_04475 at 940.865 kb on + strand, within IAI47_04475 at 940.865 kb on + strand, within IAI47_04475 at 940.865 kb on + strand, within IAI47_04475 at 940.866 kb on - strand, within IAI47_04475 at 940.866 kb on - strand, within IAI47_04475 at 940.866 kb on - strand, within IAI47_04475 at 940.866 kb on - strand, within IAI47_04475 at 940.866 kb on - strand, within IAI47_04475 at 940.866 kb on - strand, within IAI47_04475 at 940.966 kb on + strand, within IAI47_04475 at 940.966 kb on + strand, within IAI47_04475 at 940.967 kb on - strand, within IAI47_04475 at 940.967 kb on - strand, within IAI47_04475 at 941.064 kb on + strand, within IAI47_04475 at 941.064 kb on + strand, within IAI47_04475 at 941.097 kb on + strand, within IAI47_04475 at 941.097 kb on + strand, within IAI47_04475 at 941.136 kb on + strand, within IAI47_04475 at 941.137 kb on - strand, within IAI47_04475 at 941.137 kb on - strand, within IAI47_04475 at 941.161 kb on + strand, within IAI47_04475 at 941.161 kb on + strand, within IAI47_04475 at 941.162 kb on - strand, within IAI47_04475 at 941.194 kb on - strand, within IAI47_04475 at 941.237 kb on + strand, within IAI47_04475 at 941.237 kb on + strand, within IAI47_04475 at 941.237 kb on + strand, within IAI47_04475 at 941.237 kb on + strand, within IAI47_04475 at 941.237 kb on + strand, within IAI47_04475 at 941.238 kb on - strand, within IAI47_04475 at 941.238 kb on - strand, within IAI47_04475 at 941.253 kb on + strand, within IAI47_04475 at 941.253 kb on + strand, within IAI47_04475 at 941.253 kb on + strand, within IAI47_04475 at 941.254 kb on - strand, within IAI47_04475 at 941.322 kb on + strand, within IAI47_04475 at 941.322 kb on + strand, within IAI47_04475 at 941.323 kb on - strand, within IAI47_04475 at 941.323 kb on - strand, within IAI47_04475 at 941.323 kb on - strand, within IAI47_04475 at 941.323 kb on - strand, within IAI47_04475 at 941.323 kb on - strand, within IAI47_04475 at 941.418 kb on + strand, within IAI47_04475 at 941.418 kb on + strand, within IAI47_04475 at 941.418 kb on + strand, within IAI47_04475 at 941.418 kb on + strand, within IAI47_04475 at 941.418 kb on + strand, within IAI47_04475 at 941.476 kb on + strand, within IAI47_04475 at 941.476 kb on + strand, within IAI47_04475 at 941.476 kb on + strand, within IAI47_04475 at 941.476 kb on + strand, within IAI47_04475 at 941.477 kb on - strand, within IAI47_04475 at 941.477 kb on - strand, within IAI47_04475 at 941.499 kb on - strand, within IAI47_04475 at 941.506 kb on + strand, within IAI47_04475 at 941.506 kb on + strand, within IAI47_04475 at 941.506 kb on + strand, within IAI47_04475 at 941.506 kb on + strand, within IAI47_04475 at 941.506 kb on + strand, within IAI47_04475 at 941.506 kb on + strand, within IAI47_04475 at 941.506 kb on + strand, within IAI47_04475 at 941.506 kb on + strand, within IAI47_04475 at 941.506 kb on + strand, within IAI47_04475 at 941.507 kb on - strand, within IAI47_04475 at 941.507 kb on - strand, within IAI47_04475 at 941.507 kb on - strand, within IAI47_04475 at 941.507 kb on - strand, within IAI47_04475 at 941.507 kb on - strand, within IAI47_04475 at 941.507 kb on - strand, within IAI47_04475 at 941.507 kb on - strand, within IAI47_04475 at 941.507 kb on - strand, within IAI47_04475 at 941.507 kb on - strand, within IAI47_04475 at 941.526 kb on + strand, within IAI47_04475 at 941.642 kb on + strand, within IAI47_04475 at 941.642 kb on + strand, within IAI47_04475 at 941.642 kb on + strand, within IAI47_04475 at 941.643 kb on - strand, within IAI47_04475 at 941.653 kb on + strand, within IAI47_04475 at 941.654 kb on - strand, within IAI47_04475 at 941.795 kb on + strand, within IAI47_04475 at 941.796 kb on - strand, within IAI47_04475 at 941.811 kb on + strand, within IAI47_04475 at 941.811 kb on + strand, within IAI47_04475 at 941.811 kb on + strand, within IAI47_04475 at 941.811 kb on + strand, within IAI47_04475 at 941.811 kb on + strand, within IAI47_04475 at 941.812 kb on - strand, within IAI47_04475 at 941.812 kb on - strand, within IAI47_04475 at 941.812 kb on - strand, within IAI47_04475 at 941.866 kb on - strand, within IAI47_04475 at 941.866 kb on - strand, within IAI47_04475 at 941.932 kb on - strand, within IAI47_04475 at 941.932 kb on - strand, within IAI47_04475 at 941.950 kb on + strand, within IAI47_04475 at 941.951 kb on - strand, within IAI47_04475 at 941.951 kb on - strand, within IAI47_04475 at 941.951 kb on - strand, within IAI47_04475 at 942.013 kb on - strand, within IAI47_04475 at 942.013 kb on - strand, within IAI47_04475 at 942.013 kb on - strand, within IAI47_04475 at 942.013 kb on - strand, within IAI47_04475 at 942.019 kb on + strand, within IAI47_04475 at 942.019 kb on + strand, within IAI47_04475 at 942.019 kb on + strand, within IAI47_04475 at 942.019 kb on + strand, within IAI47_04475 at 942.019 kb on + strand, within IAI47_04475 at 942.020 kb on - strand, within IAI47_04475 at 942.020 kb on - strand, within IAI47_04475 at 942.020 kb on - strand, within IAI47_04475 at 942.068 kb on + strand, within IAI47_04475 at 942.068 kb on + strand, within IAI47_04475 at 942.095 kb on - strand, within IAI47_04475 at 942.095 kb on - strand, within IAI47_04475 at 942.194 kb on + strand, within IAI47_04475 at 942.194 kb on + strand, within IAI47_04475 at 942.195 kb on - strand, within IAI47_04475 at 942.195 kb on - strand, within IAI47_04475 at 942.273 kb on + strand, within IAI47_04475 at 942.273 kb on + strand, within IAI47_04475 at 942.274 kb on - strand, within IAI47_04475 at 942.274 kb on - strand, within IAI47_04475 at 942.280 kb on + strand, within IAI47_04475 at 942.280 kb on + strand, within IAI47_04475 at 942.281 kb on - strand, within IAI47_04475 at 942.439 kb on - strand, within IAI47_04475 at 942.500 kb on + strand, within IAI47_04475 at 942.500 kb on + strand, within IAI47_04475 at 942.501 kb on - strand, within IAI47_04475 at 942.589 kb on - strand, within IAI47_04475 at 942.609 kb on + strand, within IAI47_04475 at 942.636 kb on + strand, within IAI47_04475 at 942.636 kb on + strand, within IAI47_04475 at 942.636 kb on + strand, within IAI47_04475 at 942.636 kb on + strand, within IAI47_04475 at 942.637 kb on - strand, within IAI47_04475 at 942.637 kb on - strand, within IAI47_04475 at 942.639 kb on + strand, within IAI47_04475 at 942.639 kb on + strand, within IAI47_04475 at 942.639 kb on + strand, within IAI47_04475 at 942.640 kb on - strand, within IAI47_04475 at 942.640 kb on - strand, within IAI47_04475 at 942.640 kb on - strand, within IAI47_04475 at 942.640 kb on - strand, within IAI47_04475 at 942.640 kb on - strand, within IAI47_04475 at 942.663 kb on + strand, within IAI47_04475 at 942.664 kb on - strand, within IAI47_04475 at 942.664 kb on - strand, within IAI47_04475 at 942.763 kb on + strand, within IAI47_04475 at 942.763 kb on + strand, within IAI47_04475 at 942.763 kb on + strand, within IAI47_04475 at 942.763 kb on + strand, within IAI47_04475 at 942.764 kb on - strand, within IAI47_04475 at 942.764 kb on - strand, within IAI47_04475 at 942.764 kb on - strand, within IAI47_04475 at 942.764 kb on - strand, within IAI47_04475 at 942.764 kb on - strand, within IAI47_04475 at 942.774 kb on + strand, within IAI47_04475 at 942.774 kb on + strand, within IAI47_04475 at 942.775 kb on - strand, within IAI47_04475 at 942.775 kb on - strand, within IAI47_04475 at 942.775 kb on - strand, within IAI47_04475 at 942.775 kb on - strand, within IAI47_04475 at 942.775 kb on - strand, within IAI47_04475 at 942.775 kb on - strand, within IAI47_04475 at 942.826 kb on - strand, within IAI47_04475 at 942.876 kb on + strand at 942.876 kb on + strand at 942.877 kb on - strand at 942.877 kb on - strand at 942.891 kb on + strand at 942.922 kb on + strand at 942.923 kb on - strand at 942.923 kb on - strand at 942.923 kb on - strand at 942.966 kb on + strand at 942.966 kb on + strand at 942.966 kb on + strand at 942.966 kb on + strand at 942.967 kb on - strand at 942.967 kb on - strand at 942.968 kb on + strand at 942.969 kb on - strand at 942.969 kb on - strand at 943.029 kb on + strand at 943.029 kb on + strand at 943.029 kb on + strand at 943.029 kb on + strand at 943.029 kb on + strand at 943.029 kb on + strand at 943.029 kb on + strand at 943.030 kb on - strand at 943.030 kb on - strand at 943.030 kb on - strand at 943.034 kb on + strand at 943.034 kb on + strand at 943.083 kb on + strand at 943.090 kb on + strand at 943.090 kb on + strand at 943.090 kb on + strand at 943.090 kb on + strand at 943.091 kb on - strand at 943.154 kb on - strand at 943.261 kb on - strand at 943.296 kb on + strand at 943.297 kb on - strand at 943.328 kb on + strand, within IAI47_04480 at 943.328 kb on + strand, within IAI47_04480 at 943.329 kb on - strand, within IAI47_04480 at 943.329 kb on - strand, within IAI47_04480 at 943.329 kb on - strand, within IAI47_04480 at 943.329 kb on - strand, within IAI47_04480 at 943.329 kb on - strand, within IAI47_04480 at 943.329 kb on - strand, within IAI47_04480 at 943.390 kb on + strand, within IAI47_04480 at 943.390 kb on + strand, within IAI47_04480 at 943.426 kb on + strand, within IAI47_04480 at 943.426 kb on + strand, within IAI47_04480 at 943.426 kb on + strand, within IAI47_04480 at 943.426 kb on + strand, within IAI47_04480 at 943.426 kb on + strand, within IAI47_04480 at 943.426 kb on + strand at 943.426 kb on + strand, within IAI47_04480 at 943.427 kb on - strand, within IAI47_04480 at 943.427 kb on - strand, within IAI47_04480 at 943.427 kb on - strand, within IAI47_04480 at 943.427 kb on - strand, within IAI47_04480 at 943.427 kb on - strand, within IAI47_04480 at 943.427 kb on - strand, within IAI47_04480 at 943.427 kb on - strand, within IAI47_04480 at 943.427 kb on - strand, within IAI47_04480 at 943.427 kb on - strand, within IAI47_04480 at 943.427 kb on - strand, within IAI47_04480 at 943.427 kb on - strand, within IAI47_04480 at 943.427 kb on - strand, within IAI47_04480 at 943.427 kb on - strand, within IAI47_04480 at 943.428 kb on + strand, within IAI47_04480 at 943.429 kb on - strand, within IAI47_04480 at 943.429 kb on - strand, within IAI47_04480 at 943.429 kb on - strand, within IAI47_04480 at 943.534 kb on + strand, within IAI47_04480 at 943.534 kb on + strand, within IAI47_04480 at 943.535 kb on - strand, within IAI47_04480 at 943.535 kb on - strand, within IAI47_04480 at 943.535 kb on - strand, within IAI47_04480 at 943.596 kb on + strand, within IAI47_04480 at 943.626 kb on + strand, within IAI47_04480 at 943.627 kb on - strand, within IAI47_04480 at 943.708 kb on + strand, within IAI47_04480 at 943.708 kb on + strand, within IAI47_04480 at 943.927 kb on + strand at 943.928 kb on - strand at 943.928 kb on - strand at 943.928 kb on - strand at 943.928 kb on - strand at 943.928 kb on - strand at 943.967 kb on + strand at 943.968 kb on - strand at 944.014 kb on + strand at 944.014 kb on + strand at 944.014 kb on + strand at 944.014 kb on + strand at 944.014 kb on + strand at 944.014 kb on + strand at 944.015 kb on - strand at 944.015 kb on - strand at 944.015 kb on - strand at 944.015 kb on - strand at 944.015 kb on - strand at 944.017 kb on + strand at 944.017 kb on + strand at 944.017 kb on + strand at 944.017 kb on + strand at 944.017 kb on + strand at 944.018 kb on - strand at 944.020 kb on + strand at 944.020 kb on + strand at 944.020 kb on + strand at 944.021 kb on - strand at 944.021 kb on - strand at 944.021 kb on - strand at 944.021 kb on - strand at 944.045 kb on + strand at 944.072 kb on + strand, within IAI47_04485 at 944.072 kb on + strand, within IAI47_04485 at 944.072 kb on + strand, within IAI47_04485 at 944.072 kb on + strand, within IAI47_04485 at 944.072 kb on + strand, within IAI47_04485 at 944.072 kb on + strand, within IAI47_04485 at 944.072 kb on + strand, within IAI47_04485 at 944.073 kb on - strand, within IAI47_04485 at 944.100 kb on - strand, within IAI47_04485 at 944.114 kb on + strand, within IAI47_04485 at 944.115 kb on - strand, within IAI47_04485 at 944.115 kb on - strand, within IAI47_04485 at 944.115 kb on - strand, within IAI47_04485 at 944.115 kb on - strand, within IAI47_04485 at 944.115 kb on - strand, within IAI47_04485
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 940,412 - -1.1 940,456 - +0.8 940,456 - +0.4 940,503 + +0.0 940,504 - -0.3 940,536 + -0.8 940,537 - -0.6 940,537 - +1.1 940,537 - -0.8 940,554 + -0.8 940,554 + +1.5 940,555 - +1.4 940,585 - +0.0 940,620 - +1.7 940,620 - +0.7 940,708 - -0.6 940,708 - +0.7 940,723 - IAI47_04475 0.10 +0.4 940,733 - IAI47_04475 0.11 +0.5 940,796 + IAI47_04475 0.13 +0.8 940,799 + IAI47_04475 0.13 +0.1 940,812 + IAI47_04475 0.14 +1.7 940,812 + IAI47_04475 0.14 +1.0 940,813 - IAI47_04475 0.14 -1.3 940,856 + IAI47_04475 0.15 -1.2 940,862 + IAI47_04475 0.16 +2.1 940,863 - IAI47_04475 0.16 +0.3 940,865 + IAI47_04475 0.16 +0.8 940,865 + IAI47_04475 0.16 -1.0 940,865 + IAI47_04475 0.16 +0.3 940,865 + IAI47_04475 0.16 +1.2 940,866 - IAI47_04475 0.16 -1.5 940,866 - IAI47_04475 0.16 +0.4 940,866 - IAI47_04475 0.16 -0.0 940,866 - IAI47_04475 0.16 +1.0 940,866 - IAI47_04475 0.16 +0.9 940,866 - IAI47_04475 0.16 +1.2 940,966 + IAI47_04475 0.20 -1.0 940,966 + IAI47_04475 0.20 -1.0 940,967 - IAI47_04475 0.20 -1.6 940,967 - IAI47_04475 0.20 -0.5 941,064 + IAI47_04475 0.23 -2.0 941,064 + IAI47_04475 0.23 -1.7 941,097 + IAI47_04475 0.24 -1.1 941,097 + IAI47_04475 0.24 +0.3 941,136 + IAI47_04475 0.26 -2.2 941,137 - IAI47_04475 0.26 -0.7 941,137 - IAI47_04475 0.26 +1.7 941,161 + IAI47_04475 0.27 +0.3 941,161 + IAI47_04475 0.27 +0.1 941,162 - IAI47_04475 0.27 +1.2 941,194 - IAI47_04475 0.28 -0.7 941,237 + IAI47_04475 0.30 +0.4 941,237 + IAI47_04475 0.30 -0.1 941,237 + IAI47_04475 0.30 +1.5 941,237 + IAI47_04475 0.30 +3.2 941,237 + IAI47_04475 0.30 +1.6 941,238 - IAI47_04475 0.30 -0.9 941,238 - IAI47_04475 0.30 -1.2 941,253 + IAI47_04475 0.30 +0.1 941,253 + IAI47_04475 0.30 +0.3 941,253 + IAI47_04475 0.30 -0.7 941,254 - IAI47_04475 0.30 -1.8 941,322 + IAI47_04475 0.33 -1.7 941,322 + IAI47_04475 0.33 +0.2 941,323 - IAI47_04475 0.33 +2.1 941,323 - IAI47_04475 0.33 -0.7 941,323 - IAI47_04475 0.33 +0.1 941,323 - IAI47_04475 0.33 -0.5 941,323 - IAI47_04475 0.33 +0.9 941,418 + IAI47_04475 0.36 +1.0 941,418 + IAI47_04475 0.36 +1.1 941,418 + IAI47_04475 0.36 +0.8 941,418 + IAI47_04475 0.36 +1.9 941,418 + IAI47_04475 0.36 -0.8 941,476 + IAI47_04475 0.39 +1.1 941,476 + IAI47_04475 0.39 +0.3 941,476 + IAI47_04475 0.39 -1.0 941,476 + IAI47_04475 0.39 +1.4 941,477 - IAI47_04475 0.39 +0.5 941,477 - IAI47_04475 0.39 +0.3 941,499 - IAI47_04475 0.39 -0.6 941,506 + IAI47_04475 0.40 +0.3 941,506 + IAI47_04475 0.40 +0.1 941,506 + IAI47_04475 0.40 +1.3 941,506 + IAI47_04475 0.40 +0.4 941,506 + IAI47_04475 0.40 +0.2 941,506 + IAI47_04475 0.40 -0.4 941,506 + IAI47_04475 0.40 -2.1 941,506 + IAI47_04475 0.40 -1.0 941,506 + IAI47_04475 0.40 +0.2 941,507 - IAI47_04475 0.40 -0.9 941,507 - IAI47_04475 0.40 +0.8 941,507 - IAI47_04475 0.40 -0.3 941,507 - IAI47_04475 0.40 +0.2 941,507 - IAI47_04475 0.40 -0.8 941,507 - IAI47_04475 0.40 +0.7 941,507 - IAI47_04475 0.40 +1.7 941,507 - IAI47_04475 0.40 +3.1 941,507 - IAI47_04475 0.40 -0.5 941,526 + IAI47_04475 0.40 -0.6 941,642 + IAI47_04475 0.45 +1.2 941,642 + IAI47_04475 0.45 -0.5 941,642 + IAI47_04475 0.45 -0.5 941,643 - IAI47_04475 0.45 +1.4 941,653 + IAI47_04475 0.45 +2.5 941,654 - IAI47_04475 0.45 +0.0 941,795 + IAI47_04475 0.50 +0.8 941,796 - IAI47_04475 0.51 +0.6 941,811 + IAI47_04475 0.51 -1.8 941,811 + IAI47_04475 0.51 +0.1 941,811 + IAI47_04475 0.51 +0.3 941,811 + IAI47_04475 0.51 +0.5 941,811 + IAI47_04475 0.51 -1.1 941,812 - IAI47_04475 0.51 +0.2 941,812 - IAI47_04475 0.51 +1.1 941,812 - IAI47_04475 0.51 +1.2 941,866 - IAI47_04475 0.53 +0.0 941,866 - IAI47_04475 0.53 +1.8 941,932 - IAI47_04475 0.56 +0.8 941,932 - IAI47_04475 0.56 +1.1 941,950 + IAI47_04475 0.56 +2.9 941,951 - IAI47_04475 0.56 -1.9 941,951 - IAI47_04475 0.56 -1.0 941,951 - IAI47_04475 0.56 +2.3 942,013 - IAI47_04475 0.59 +0.8 942,013 - IAI47_04475 0.59 +0.6 942,013 - IAI47_04475 0.59 +0.6 942,013 - IAI47_04475 0.59 -1.0 942,019 + IAI47_04475 0.59 -0.3 942,019 + IAI47_04475 0.59 -2.1 942,019 + IAI47_04475 0.59 +2.2 942,019 + IAI47_04475 0.59 -1.5 942,019 + IAI47_04475 0.59 -0.9 942,020 - 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Or see this region's nucleotide sequence