Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_01755 and IAI47_01760 are separated by 29 nucleotides IAI47_01760 and IAI47_01765 are separated by 30 nucleotides IAI47_01765 and IAI47_01770 are separated by 160 nucleotides
IAI47_01755: IAI47_01755 - thiosulfate sulfurtransferase GlpE, at 384,072 to 384,398
_01755
IAI47_01760: IAI47_01760 - rhomboid family intramembrane serine protease GlpG, at 384,428 to 385,249
_01760
IAI47_01765: IAI47_01765 - DeoR/GlpR family transcriptional regulator, at 385,280 to 386,038
_01765
IAI47_01770: IAI47_01770 - maltodextrin phosphorylase, at 386,199 to 388,604
_01770
Position (kb) 385
386
387 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 384.297 kb on + strand, within IAI47_01755 at 384.298 kb on - strand, within IAI47_01755 at 384.327 kb on + strand, within IAI47_01755 at 384.328 kb on - strand, within IAI47_01755 at 384.396 kb on + strand at 384.396 kb on + strand at 384.396 kb on + strand at 384.397 kb on - strand at 384.397 kb on - strand at 384.397 kb on - strand at 384.397 kb on - strand at 384.397 kb on - strand at 384.397 kb on - strand at 384.397 kb on - strand at 384.423 kb on + strand at 384.423 kb on + strand at 384.423 kb on + strand at 384.423 kb on + strand at 384.423 kb on + strand at 384.424 kb on - strand at 384.424 kb on - strand at 384.424 kb on - strand at 384.424 kb on - strand at 384.424 kb on - strand at 384.424 kb on - strand at 384.424 kb on - strand at 384.437 kb on - strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.479 kb on + strand at 384.480 kb on - strand at 384.480 kb on - strand at 384.480 kb on - strand at 384.480 kb on - strand at 384.480 kb on - strand at 384.527 kb on + strand, within IAI47_01760 at 384.528 kb on - strand, within IAI47_01760 at 384.528 kb on - strand, within IAI47_01760 at 384.528 kb on - strand, within IAI47_01760 at 384.662 kb on + strand, within IAI47_01760 at 384.663 kb on - strand, within IAI47_01760 at 384.676 kb on + strand, within IAI47_01760 at 384.676 kb on + strand, within IAI47_01760 at 384.676 kb on + strand, within IAI47_01760 at 384.677 kb on - strand, within IAI47_01760 at 384.677 kb on - strand, within IAI47_01760 at 384.748 kb on + strand, within IAI47_01760 at 384.748 kb on + strand, within IAI47_01760 at 384.749 kb on - strand, within IAI47_01760 at 384.749 kb on - strand, within IAI47_01760 at 384.749 kb on - strand, within IAI47_01760 at 384.880 kb on + strand, within IAI47_01760 at 384.881 kb on - strand, within IAI47_01760 at 384.899 kb on + strand, within IAI47_01760 at 384.899 kb on + strand, within IAI47_01760 at 384.899 kb on + strand, within IAI47_01760 at 384.899 kb on + strand, within IAI47_01760 at 384.900 kb on - strand, within IAI47_01760 at 384.900 kb on - strand, within IAI47_01760 at 384.900 kb on - strand, within IAI47_01760 at 384.900 kb on - strand, within IAI47_01760 at 384.948 kb on + strand, within IAI47_01760 at 384.948 kb on + strand, within IAI47_01760 at 384.948 kb on + strand at 384.948 kb on + strand, within IAI47_01760 at 384.949 kb on - strand, within IAI47_01760 at 384.949 kb on - strand, within IAI47_01760 at 384.949 kb on - strand, within IAI47_01760 at 384.953 kb on - strand, within IAI47_01760 at 384.953 kb on - strand, within IAI47_01760 at 384.953 kb on - strand, within IAI47_01760 at 384.953 kb on - strand, within IAI47_01760 at 385.034 kb on + strand, within IAI47_01760 at 385.034 kb on + strand, within IAI47_01760 at 385.035 kb on - strand, within IAI47_01760 at 385.050 kb on - strand, within IAI47_01760 at 385.091 kb on + strand, within IAI47_01760 at 385.092 kb on - strand, within IAI47_01760 at 385.092 kb on - strand, within IAI47_01760 at 385.092 kb on - strand, within IAI47_01760 at 385.092 kb on - strand, within IAI47_01760 at 385.092 kb on - strand, within IAI47_01760 at 385.247 kb on + strand at 385.317 kb on + strand at 385.317 kb on + strand at 385.318 kb on - strand at 385.334 kb on + strand at 385.334 kb on + strand at 385.334 kb on + strand at 385.334 kb on + strand at 385.334 kb on + strand at 385.334 kb on + strand at 385.334 kb on + strand at 385.334 kb on + strand at 385.335 kb on - strand at 385.335 kb on - strand at 385.335 kb on - strand at 385.335 kb on - strand at 385.335 kb on - strand at 385.335 kb on - strand at 385.335 kb on - strand at 385.335 kb on - strand at 385.342 kb on + strand at 385.356 kb on + strand, within IAI47_01765 at 385.356 kb on + strand, within IAI47_01765 at 385.356 kb on + strand, within IAI47_01765 at 385.356 kb on + strand, within IAI47_01765 at 385.357 kb on - strand, within IAI47_01765 at 385.357 kb on - strand, within IAI47_01765 at 385.357 kb on - strand, within IAI47_01765 at 385.357 kb on - strand, within IAI47_01765 at 385.357 kb on - strand, within IAI47_01765 at 385.357 kb on - strand, within IAI47_01765 at 385.567 kb on + strand, within IAI47_01765 at 385.567 kb on + strand, within IAI47_01765 at 385.567 kb on + strand, within IAI47_01765 at 385.568 kb on - strand, within IAI47_01765 at 385.568 kb on - strand, within IAI47_01765 at 385.568 kb on - strand, within IAI47_01765 at 385.568 kb on - strand, within IAI47_01765 at 385.568 kb on - strand, within IAI47_01765 at 385.573 kb on + strand, within IAI47_01765 at 385.573 kb on + strand, within IAI47_01765 at 385.573 kb on + strand, within IAI47_01765 at 385.574 kb on - strand, within IAI47_01765 at 385.626 kb on + strand, within IAI47_01765 at 385.627 kb on - strand, within IAI47_01765 at 385.627 kb on - strand, within IAI47_01765 at 385.642 kb on + strand, within IAI47_01765 at 385.643 kb on - strand, within IAI47_01765 at 385.648 kb on + strand, within IAI47_01765 at 385.649 kb on - strand, within IAI47_01765 at 385.649 kb on - strand, within IAI47_01765 at 385.756 kb on + strand, within IAI47_01765 at 385.756 kb on + strand, within IAI47_01765 at 385.756 kb on + strand, within IAI47_01765 at 385.757 kb on - strand, within IAI47_01765 at 385.757 kb on - strand, within IAI47_01765 at 385.757 kb on - strand, within IAI47_01765 at 385.778 kb on + strand, within IAI47_01765 at 385.836 kb on - strand, within IAI47_01765 at 385.867 kb on - strand, within IAI47_01765 at 385.867 kb on - strand, within IAI47_01765 at 385.958 kb on + strand, within IAI47_01765 at 385.958 kb on + strand, within IAI47_01765 at 385.958 kb on + strand, within IAI47_01765 at 385.958 kb on + strand, within IAI47_01765 at 385.958 kb on + strand, within IAI47_01765 at 385.959 kb on - strand, within IAI47_01765 at 385.959 kb on - strand, within IAI47_01765 at 385.959 kb on - strand, within IAI47_01765 at 385.964 kb on + strand at 385.964 kb on + strand at 385.964 kb on + strand at 385.965 kb on - strand at 385.965 kb on - strand at 385.965 kb on - strand at 385.972 kb on + strand at 385.972 kb on + strand at 385.973 kb on - strand at 386.109 kb on + strand at 386.109 kb on + strand at 386.109 kb on + strand at 386.109 kb on + strand at 386.109 kb on + strand at 386.110 kb on - strand at 386.115 kb on - strand at 386.115 kb on - strand at 386.155 kb on - strand at 386.155 kb on - strand at 386.183 kb on - strand at 386.198 kb on + strand at 386.198 kb on + strand at 386.198 kb on + strand at 386.198 kb on + strand at 386.199 kb on - strand at 386.199 kb on - strand at 386.199 kb on - strand at 386.199 kb on - strand at 386.216 kb on + strand at 386.366 kb on + strand at 386.367 kb on - strand at 386.367 kb on - strand at 386.383 kb on + strand at 386.384 kb on - strand at 386.384 kb on - strand at 386.384 kb on - strand at 386.384 kb on - strand at 386.388 kb on + strand at 386.388 kb on + strand at 386.389 kb on - strand at 386.389 kb on - strand at 386.389 kb on - strand at 386.389 kb on - strand at 386.389 kb on - strand at 386.452 kb on - strand, within IAI47_01770 at 386.452 kb on - strand, within IAI47_01770 at 386.494 kb on + strand, within IAI47_01770 at 386.495 kb on - strand, within IAI47_01770 at 386.495 kb on - strand, within IAI47_01770 at 386.565 kb on + strand, within IAI47_01770 at 386.565 kb on + strand, within IAI47_01770 at 386.565 kb on + strand, within IAI47_01770 at 386.565 kb on + strand, within IAI47_01770 at 386.565 kb on + strand, within IAI47_01770 at 386.565 kb on + strand, within IAI47_01770 at 386.565 kb on + strand, within IAI47_01770 at 386.566 kb on - strand, within IAI47_01770 at 386.566 kb on - strand, within IAI47_01770 at 386.566 kb on - strand, within IAI47_01770 at 386.566 kb on - strand, within IAI47_01770 at 386.566 kb on - strand, within IAI47_01770 at 386.566 kb on - strand, within IAI47_01770 at 386.566 kb on - strand, within IAI47_01770 at 386.566 kb on - strand, within IAI47_01770 at 386.567 kb on + strand, within IAI47_01770 at 386.567 kb on + strand, within IAI47_01770 at 386.568 kb on - strand, within IAI47_01770 at 386.568 kb on - strand, within IAI47_01770 at 386.619 kb on + strand, within IAI47_01770 at 386.619 kb on + strand, within IAI47_01770 at 386.619 kb on + strand, within IAI47_01770 at 386.620 kb on - strand, within IAI47_01770 at 386.658 kb on - strand, within IAI47_01770 at 386.698 kb on - strand, within IAI47_01770 at 386.779 kb on + strand, within IAI47_01770 at 386.779 kb on + strand, within IAI47_01770 at 386.779 kb on + strand, within IAI47_01770 at 386.948 kb on + strand, within IAI47_01770 at 386.949 kb on - strand, within IAI47_01770 at 386.979 kb on + strand, within IAI47_01770 at 386.979 kb on + strand, within IAI47_01770 at 386.979 kb on + strand, within IAI47_01770 at 386.980 kb on - strand, within IAI47_01770 at 386.980 kb on - strand, within IAI47_01770 at 386.980 kb on - strand, within IAI47_01770 at 387.031 kb on - strand, within IAI47_01770 at 387.031 kb on - strand, within IAI47_01770
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 384,297 + IAI47_01755 0.69 +0.8 384,298 - IAI47_01755 0.69 -0.1 384,327 + IAI47_01755 0.78 +0.1 384,328 - IAI47_01755 0.78 +1.1 384,396 + +0.1 384,396 + -1.6 384,396 + -0.4 384,397 - +1.2 384,397 - +1.0 384,397 - -1.0 384,397 - +1.8 384,397 - +0.2 384,397 - +1.2 384,397 - +1.1 384,423 + -0.5 384,423 + -0.2 384,423 + -0.4 384,423 + -2.3 384,423 + -1.3 384,424 - +0.2 384,424 - -1.8 384,424 - -1.1 384,424 - -0.7 384,424 - -1.0 384,424 - +0.7 384,424 - -1.8 384,437 - +0.6 384,479 + +0.2 384,479 + +0.3 384,479 + +0.2 384,479 + -2.1 384,479 + +0.6 384,479 + -1.7 384,479 + -0.0 384,479 + -0.5 384,479 + -0.3 384,479 + -0.4 384,479 + +0.4 384,479 + -0.8 384,480 - +1.0 384,480 - -0.6 384,480 - -0.3 384,480 - +1.8 384,480 - -0.7 384,527 + IAI47_01760 0.12 -0.5 384,528 - IAI47_01760 0.12 +1.0 384,528 - IAI47_01760 0.12 +0.5 384,528 - IAI47_01760 0.12 +1.7 384,662 + IAI47_01760 0.28 -1.7 384,663 - IAI47_01760 0.29 -1.6 384,676 + IAI47_01760 0.30 -1.1 384,676 + IAI47_01760 0.30 -0.8 384,676 + IAI47_01760 0.30 -0.0 384,677 - IAI47_01760 0.30 -0.2 384,677 - IAI47_01760 0.30 +1.6 384,748 + IAI47_01760 0.39 +0.5 384,748 + IAI47_01760 0.39 +0.7 384,749 - IAI47_01760 0.39 -1.0 384,749 - IAI47_01760 0.39 -1.2 384,749 - IAI47_01760 0.39 -1.0 384,880 + IAI47_01760 0.55 +0.1 384,881 - IAI47_01760 0.55 -0.5 384,899 + IAI47_01760 0.57 +0.2 384,899 + IAI47_01760 0.57 -1.7 384,899 + IAI47_01760 0.57 +0.9 384,899 + IAI47_01760 0.57 +0.0 384,900 - IAI47_01760 0.57 -0.5 384,900 - IAI47_01760 0.57 -0.2 384,900 - IAI47_01760 0.57 +2.8 384,900 - IAI47_01760 0.57 -1.0 384,948 + IAI47_01760 0.63 -0.2 384,948 + IAI47_01760 0.63 -0.0 384,948 + +0.4 384,948 + IAI47_01760 0.63 -1.5 384,949 - IAI47_01760 0.63 +1.7 384,949 - IAI47_01760 0.63 +1.0 384,949 - IAI47_01760 0.63 +0.6 384,953 - IAI47_01760 0.64 -0.4 384,953 - IAI47_01760 0.64 -0.0 384,953 - IAI47_01760 0.64 -0.3 384,953 - IAI47_01760 0.64 -0.6 385,034 + IAI47_01760 0.74 +0.5 385,034 + IAI47_01760 0.74 -1.6 385,035 - IAI47_01760 0.74 -0.2 385,050 - IAI47_01760 0.76 -1.4 385,091 + IAI47_01760 0.81 -0.8 385,092 - IAI47_01760 0.81 +0.6 385,092 - IAI47_01760 0.81 +1.6 385,092 - IAI47_01760 0.81 -0.1 385,092 - IAI47_01760 0.81 -0.4 385,092 - IAI47_01760 0.81 -1.0 385,247 + +1.0 385,317 + +1.6 385,317 + -1.6 385,318 - -0.1 385,334 + +0.6 385,334 + +1.2 385,334 + +1.0 385,334 + +0.0 385,334 + +0.3 385,334 + -0.0 385,334 + +1.2 385,334 + +0.2 385,335 - -0.7 385,335 - +0.5 385,335 - -0.1 385,335 - -2.2 385,335 - +0.5 385,335 - +0.4 385,335 - -1.1 385,335 - -0.5 385,342 + -0.9 385,356 + IAI47_01765 0.10 -1.1 385,356 + IAI47_01765 0.10 +1.5 385,356 + IAI47_01765 0.10 +0.2 385,356 + IAI47_01765 0.10 -0.9 385,357 - IAI47_01765 0.10 +0.2 385,357 - IAI47_01765 0.10 -0.1 385,357 - IAI47_01765 0.10 -0.8 385,357 - IAI47_01765 0.10 +1.1 385,357 - IAI47_01765 0.10 +0.0 385,357 - IAI47_01765 0.10 +0.6 385,567 + IAI47_01765 0.38 +0.0 385,567 + IAI47_01765 0.38 +0.0 385,567 + IAI47_01765 0.38 -2.2 385,568 - IAI47_01765 0.38 -0.3 385,568 - IAI47_01765 0.38 -0.5 385,568 - IAI47_01765 0.38 +0.1 385,568 - IAI47_01765 0.38 +1.0 385,568 - IAI47_01765 0.38 -1.0 385,573 + IAI47_01765 0.39 -0.3 385,573 + IAI47_01765 0.39 -1.2 385,573 + IAI47_01765 0.39 +0.5 385,574 - IAI47_01765 0.39 +0.8 385,626 + IAI47_01765 0.46 -0.2 385,627 - IAI47_01765 0.46 +0.3 385,627 - IAI47_01765 0.46 +0.2 385,642 + IAI47_01765 0.48 +1.8 385,643 - IAI47_01765 0.48 -0.1 385,648 + IAI47_01765 0.48 -1.0 385,649 - IAI47_01765 0.49 -0.9 385,649 - IAI47_01765 0.49 +1.1 385,756 + IAI47_01765 0.63 -0.4 385,756 + IAI47_01765 0.63 +0.5 385,756 + IAI47_01765 0.63 -0.8 385,757 - IAI47_01765 0.63 +0.2 385,757 - IAI47_01765 0.63 +0.5 385,757 - IAI47_01765 0.63 -0.3 385,778 + IAI47_01765 0.66 -0.6 385,836 - IAI47_01765 0.73 +0.2 385,867 - IAI47_01765 0.77 -0.5 385,867 - IAI47_01765 0.77 +0.5 385,958 + IAI47_01765 0.89 -1.5 385,958 + IAI47_01765 0.89 +0.3 385,958 + IAI47_01765 0.89 +0.2 385,958 + IAI47_01765 0.89 -1.0 385,958 + IAI47_01765 0.89 +1.1 385,959 - IAI47_01765 0.89 +0.3 385,959 - IAI47_01765 0.89 -0.5 385,959 - IAI47_01765 0.89 -0.6 385,964 + +0.3 385,964 + -1.6 385,964 + -0.6 385,965 - +1.6 385,965 - +0.2 385,965 - -0.5 385,972 + +0.2 385,972 + +1.2 385,973 - +0.9 386,109 + -0.4 386,109 + -0.8 386,109 + +1.4 386,109 + -2.3 386,109 + -2.7 386,110 - +1.0 386,115 - +1.3 386,115 - +0.2 386,155 - -1.0 386,155 - +2.8 386,183 - +1.4 386,198 + +1.6 386,198 + +0.3 386,198 + +2.5 386,198 + +0.2 386,199 - +1.3 386,199 - +0.7 386,199 - +0.1 386,199 - +0.5 386,216 + -0.7 386,366 + +0.6 386,367 - -0.2 386,367 - +2.4 386,383 + +0.1 386,384 - +0.1 386,384 - +1.8 386,384 - -0.6 386,384 - -0.0 386,388 + +0.5 386,388 + +0.5 386,389 - -2.3 386,389 - +0.3 386,389 - +3.6 386,389 - +0.4 386,389 - -1.6 386,452 - IAI47_01770 0.11 -1.7 386,452 - IAI47_01770 0.11 -0.3 386,494 + IAI47_01770 0.12 -0.9 386,495 - IAI47_01770 0.12 -1.2 386,495 - IAI47_01770 0.12 +0.3 386,565 + IAI47_01770 0.15 +1.7 386,565 + IAI47_01770 0.15 -0.4 386,565 + IAI47_01770 0.15 +0.6 386,565 + IAI47_01770 0.15 +1.1 386,565 + IAI47_01770 0.15 -0.9 386,565 + IAI47_01770 0.15 -0.3 386,565 + IAI47_01770 0.15 -0.2 386,566 - IAI47_01770 0.15 -0.4 386,566 - IAI47_01770 0.15 -0.2 386,566 - IAI47_01770 0.15 +1.2 386,566 - IAI47_01770 0.15 -1.9 386,566 - IAI47_01770 0.15 +0.9 386,566 - IAI47_01770 0.15 +1.8 386,566 - IAI47_01770 0.15 -1.1 386,566 - IAI47_01770 0.15 -0.2 386,567 + IAI47_01770 0.15 -0.3 386,567 + IAI47_01770 0.15 +1.1 386,568 - IAI47_01770 0.15 +0.8 386,568 - IAI47_01770 0.15 -0.9 386,619 + IAI47_01770 0.17 +0.9 386,619 + IAI47_01770 0.17 -0.0 386,619 + IAI47_01770 0.17 +0.1 386,620 - IAI47_01770 0.17 -1.6 386,658 - IAI47_01770 0.19 -0.2 386,698 - IAI47_01770 0.21 +0.8 386,779 + IAI47_01770 0.24 -1.2 386,779 + IAI47_01770 0.24 -0.5 386,779 + IAI47_01770 0.24 +1.0 386,948 + IAI47_01770 0.31 -1.0 386,949 - IAI47_01770 0.31 +0.0 386,979 + IAI47_01770 0.32 +0.6 386,979 + IAI47_01770 0.32 +0.3 386,979 + IAI47_01770 0.32 -1.5 386,980 - IAI47_01770 0.32 -1.9 386,980 - IAI47_01770 0.32 -0.9 386,980 - IAI47_01770 0.32 -0.6 387,031 - IAI47_01770 0.35 +0.2 387,031 - IAI47_01770 0.35 -2.1
Or see this region's nucleotide sequence