Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_19270 and IAI47_19275 are separated by 116 nucleotides IAI47_19275 and IAI47_19280 are separated by 93 nucleotides
IAI47_19270: IAI47_19270 - alpha/beta fold hydrolase, at 81,547 to 82,311
_19270
IAI47_19275: IAI47_19275 - DMT family transporter, at 82,428 to 82,962
_19275
IAI47_19280: IAI47_19280 - PLP-dependent aminotransferase family protein, at 83,056 to 84,342
_19280
Position (kb) 82
83 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 81.457 kb on + strand at 81.457 kb on + strand at 81.457 kb on + strand at 81.458 kb on - strand at 81.458 kb on - strand at 81.458 kb on - strand at 81.500 kb on - strand at 81.508 kb on + strand at 81.557 kb on - strand at 81.557 kb on - strand at 81.567 kb on - strand at 81.567 kb on - strand at 81.602 kb on + strand at 81.602 kb on + strand at 81.603 kb on - strand at 81.615 kb on + strand at 81.615 kb on + strand at 81.616 kb on - strand at 81.685 kb on + strand, within IAI47_19270 at 81.685 kb on + strand, within IAI47_19270 at 81.685 kb on + strand, within IAI47_19270 at 81.685 kb on + strand, within IAI47_19270 at 81.685 kb on + strand, within IAI47_19270 at 81.686 kb on - strand, within IAI47_19270 at 81.686 kb on - strand, within IAI47_19270 at 81.686 kb on - strand, within IAI47_19270 at 81.686 kb on - strand, within IAI47_19270 at 81.686 kb on - strand, within IAI47_19270 at 81.686 kb on - strand, within IAI47_19270 at 81.714 kb on + strand, within IAI47_19270 at 81.714 kb on + strand, within IAI47_19270 at 81.714 kb on + strand, within IAI47_19270 at 81.715 kb on - strand, within IAI47_19270 at 81.715 kb on - strand, within IAI47_19270 at 81.848 kb on + strand, within IAI47_19270 at 81.898 kb on + strand, within IAI47_19270 at 81.899 kb on - strand, within IAI47_19270 at 81.899 kb on - strand, within IAI47_19270 at 81.954 kb on + strand, within IAI47_19270 at 82.002 kb on + strand, within IAI47_19270 at 82.003 kb on - strand, within IAI47_19270 at 82.003 kb on - strand, within IAI47_19270 at 82.204 kb on - strand, within IAI47_19270 at 82.204 kb on - strand, within IAI47_19270 at 82.317 kb on - strand at 82.333 kb on + strand at 82.333 kb on + strand at 82.333 kb on + strand at 82.334 kb on - strand at 82.334 kb on - strand at 82.378 kb on + strand at 82.379 kb on - strand at 82.391 kb on - strand at 82.458 kb on - strand at 82.458 kb on - strand at 82.458 kb on - strand at 82.458 kb on - strand at 82.466 kb on + strand at 82.481 kb on + strand at 82.482 kb on - strand, within IAI47_19275 at 82.616 kb on - strand, within IAI47_19275 at 82.623 kb on + strand, within IAI47_19275 at 82.623 kb on + strand, within IAI47_19275 at 82.623 kb on + strand, within IAI47_19275 at 82.674 kb on - strand, within IAI47_19275 at 82.674 kb on - strand, within IAI47_19275 at 82.714 kb on - strand, within IAI47_19275 at 82.714 kb on - strand, within IAI47_19275 at 82.714 kb on - strand, within IAI47_19275 at 82.714 kb on - strand, within IAI47_19275 at 82.785 kb on - strand, within IAI47_19275 at 82.787 kb on + strand, within IAI47_19275 at 82.787 kb on + strand, within IAI47_19275 at 82.788 kb on - strand, within IAI47_19275 at 82.788 kb on - strand, within IAI47_19275 at 82.788 kb on - strand, within IAI47_19275 at 82.831 kb on + strand, within IAI47_19275 at 82.832 kb on - strand, within IAI47_19275 at 82.913 kb on - strand at 82.913 kb on - strand at 82.920 kb on + strand at 82.921 kb on - strand at 82.958 kb on + strand at 82.958 kb on + strand at 82.958 kb on + strand at 82.958 kb on + strand at 82.958 kb on + strand at 82.958 kb on + strand at 82.958 kb on + strand at 82.959 kb on - strand at 82.959 kb on - strand at 82.959 kb on - strand at 82.959 kb on - strand at 82.962 kb on + strand at 82.962 kb on + strand at 82.962 kb on + strand at 82.962 kb on + strand at 82.962 kb on + strand at 82.962 kb on + strand at 82.962 kb on + strand at 82.963 kb on - strand at 82.963 kb on - strand at 82.963 kb on - strand at 82.963 kb on - strand at 82.963 kb on - strand at 83.005 kb on + strand at 83.010 kb on + strand at 83.011 kb on - strand at 83.014 kb on + strand at 83.015 kb on - strand at 83.015 kb on - strand at 83.015 kb on - strand at 83.015 kb on - strand at 83.015 kb on - strand at 83.015 kb on - strand at 83.015 kb on - strand at 83.098 kb on + strand at 83.099 kb on - strand at 83.101 kb on + strand at 83.101 kb on + strand at 83.101 kb on + strand at 83.101 kb on + strand at 83.101 kb on + strand at 83.102 kb on - strand at 83.102 kb on - strand at 83.102 kb on - strand at 83.102 kb on - strand at 83.102 kb on - strand at 83.102 kb on - strand at 83.198 kb on + strand, within IAI47_19280 at 83.205 kb on + strand, within IAI47_19280 at 83.206 kb on - strand, within IAI47_19280 at 83.206 kb on - strand, within IAI47_19280 at 83.235 kb on + strand, within IAI47_19280 at 83.236 kb on - strand, within IAI47_19280 at 83.236 kb on - strand, within IAI47_19280 at 83.236 kb on - strand, within IAI47_19280 at 83.268 kb on + strand, within IAI47_19280 at 83.275 kb on + strand, within IAI47_19280 at 83.275 kb on + strand, within IAI47_19280 at 83.276 kb on - strand, within IAI47_19280 at 83.344 kb on + strand, within IAI47_19280 at 83.345 kb on - strand, within IAI47_19280 at 83.345 kb on - strand, within IAI47_19280 at 83.432 kb on + strand, within IAI47_19280 at 83.432 kb on + strand, within IAI47_19280 at 83.432 kb on + strand, within IAI47_19280 at 83.432 kb on + strand, within IAI47_19280 at 83.432 kb on + strand, within IAI47_19280 at 83.433 kb on - strand, within IAI47_19280 at 83.433 kb on - strand, within IAI47_19280 at 83.433 kb on - strand, within IAI47_19280 at 83.433 kb on - strand, within IAI47_19280 at 83.433 kb on - strand, within IAI47_19280 at 83.433 kb on - strand, within IAI47_19280 at 83.500 kb on - strand, within IAI47_19280 at 83.500 kb on - strand, within IAI47_19280 at 83.520 kb on + strand, within IAI47_19280 at 83.520 kb on + strand, within IAI47_19280 at 83.537 kb on - strand, within IAI47_19280 at 83.540 kb on + strand, within IAI47_19280 at 83.541 kb on + strand, within IAI47_19280 at 83.541 kb on + strand, within IAI47_19280 at 83.541 kb on + strand, within IAI47_19280 at 83.541 kb on + strand, within IAI47_19280 at 83.541 kb on + strand, within IAI47_19280 at 83.541 kb on + strand, within IAI47_19280 at 83.541 kb on + strand, within IAI47_19280 at 83.542 kb on - strand, within IAI47_19280 at 83.542 kb on - strand, within IAI47_19280 at 83.542 kb on - strand, within IAI47_19280 at 83.542 kb on - strand, within IAI47_19280 at 83.542 kb on - strand, within IAI47_19280 at 83.542 kb on - strand, within IAI47_19280 at 83.542 kb on - strand, within IAI47_19280 at 83.542 kb on - strand, within IAI47_19280 at 83.542 kb on - strand, within IAI47_19280 at 83.565 kb on + strand, within IAI47_19280 at 83.566 kb on - strand, within IAI47_19280 at 83.566 kb on - strand, within IAI47_19280 at 83.566 kb on - strand, within IAI47_19280 at 83.566 kb on - strand, within IAI47_19280 at 83.581 kb on - strand, within IAI47_19280 at 83.656 kb on - strand, within IAI47_19280 at 83.753 kb on + strand, within IAI47_19280 at 83.753 kb on + strand, within IAI47_19280 at 83.753 kb on + strand, within IAI47_19280 at 83.754 kb on - strand, within IAI47_19280 at 83.754 kb on - strand, within IAI47_19280 at 83.754 kb on - strand, within IAI47_19280 at 83.754 kb on - strand, within IAI47_19280 at 83.754 kb on - strand, within IAI47_19280 at 83.754 kb on - strand, within IAI47_19280 at 83.754 kb on - strand, within IAI47_19280 at 83.754 kb on - strand, within IAI47_19280 at 83.765 kb on + strand, within IAI47_19280 at 83.765 kb on + strand, within IAI47_19280 at 83.765 kb on + strand, within IAI47_19280 at 83.765 kb on + strand, within IAI47_19280 at 83.765 kb on + strand, within IAI47_19280 at 83.765 kb on + strand, within IAI47_19280 at 83.765 kb on + strand, within IAI47_19280 at 83.765 kb on + strand, within IAI47_19280 at 83.765 kb on + strand, within IAI47_19280 at 83.766 kb on - strand, within IAI47_19280 at 83.766 kb on - strand, within IAI47_19280 at 83.766 kb on - strand, within IAI47_19280 at 83.766 kb on - strand, within IAI47_19280 at 83.766 kb on - strand, within IAI47_19280 at 83.766 kb on - strand, within IAI47_19280 at 83.766 kb on - strand, within IAI47_19280 at 83.766 kb on - strand, within IAI47_19280 at 83.766 kb on - strand, within IAI47_19280 at 83.766 kb on - strand, within IAI47_19280 at 83.767 kb on + strand, within IAI47_19280 at 83.767 kb on + strand, within IAI47_19280 at 83.767 kb on + strand, within IAI47_19280 at 83.767 kb on + strand, within IAI47_19280 at 83.768 kb on - strand, within IAI47_19280 at 83.768 kb on - strand, within IAI47_19280 at 83.768 kb on - strand, within IAI47_19280 at 83.768 kb on - strand, within IAI47_19280 at 83.768 kb on - strand, within IAI47_19280 at 83.768 kb on - strand, within IAI47_19280 at 83.768 kb on - strand, within IAI47_19280 at 83.768 kb on - strand, within IAI47_19280 at 83.768 kb on - strand, within IAI47_19280 at 83.790 kb on + strand, within IAI47_19280 at 83.851 kb on + strand, within IAI47_19280 at 83.852 kb on - strand, within IAI47_19280 at 83.852 kb on - strand, within IAI47_19280 at 83.935 kb on - strand, within IAI47_19280 at 83.935 kb on - strand, within IAI47_19280 at 83.960 kb on + strand, within IAI47_19280 at 83.960 kb on + strand, within IAI47_19280
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 81,457 + -0.1 81,457 + -0.6 81,457 + +0.6 81,458 - +0.4 81,458 - +0.6 81,458 - -0.1 81,500 - +0.4 81,508 + +2.2 81,557 - -0.3 81,557 - +1.5 81,567 - +0.2 81,567 - +1.1 81,602 + +0.6 81,602 + +0.2 81,603 - +0.0 81,615 + +2.0 81,615 + +2.6 81,616 - +0.8 81,685 + IAI47_19270 0.18 -1.0 81,685 + IAI47_19270 0.18 +0.2 81,685 + IAI47_19270 0.18 -1.0 81,685 + IAI47_19270 0.18 +0.9 81,685 + IAI47_19270 0.18 -0.7 81,686 - IAI47_19270 0.18 +1.0 81,686 - IAI47_19270 0.18 -2.1 81,686 - IAI47_19270 0.18 -2.4 81,686 - IAI47_19270 0.18 -1.1 81,686 - IAI47_19270 0.18 -1.7 81,686 - IAI47_19270 0.18 -2.5 81,714 + IAI47_19270 0.22 -0.5 81,714 + IAI47_19270 0.22 -0.2 81,714 + IAI47_19270 0.22 +1.5 81,715 - IAI47_19270 0.22 -0.7 81,715 - IAI47_19270 0.22 +0.1 81,848 + IAI47_19270 0.39 -0.9 81,898 + IAI47_19270 0.46 +0.5 81,899 - IAI47_19270 0.46 +0.1 81,899 - IAI47_19270 0.46 -1.8 81,954 + IAI47_19270 0.53 +0.4 82,002 + IAI47_19270 0.59 +0.4 82,003 - IAI47_19270 0.60 -1.5 82,003 - IAI47_19270 0.60 -2.2 82,204 - IAI47_19270 0.86 -0.9 82,204 - IAI47_19270 0.86 +0.1 82,317 - -2.1 82,333 + -0.8 82,333 + -0.4 82,333 + -0.3 82,334 - +0.3 82,334 - +0.8 82,378 + +0.8 82,379 - -1.0 82,391 - +1.6 82,458 - -0.1 82,458 - +0.5 82,458 - +0.2 82,458 - -0.6 82,466 + -0.6 82,481 + +0.4 82,482 - IAI47_19275 0.10 +2.0 82,616 - IAI47_19275 0.35 +0.9 82,623 + IAI47_19275 0.36 +2.5 82,623 + IAI47_19275 0.36 -1.6 82,623 + IAI47_19275 0.36 +1.5 82,674 - IAI47_19275 0.46 +1.7 82,674 - IAI47_19275 0.46 -0.7 82,714 - IAI47_19275 0.53 +1.6 82,714 - IAI47_19275 0.53 -0.7 82,714 - IAI47_19275 0.53 +1.2 82,714 - IAI47_19275 0.53 -0.8 82,785 - IAI47_19275 0.67 +0.6 82,787 + IAI47_19275 0.67 -0.5 82,787 + IAI47_19275 0.67 +0.7 82,788 - IAI47_19275 0.67 -0.8 82,788 - IAI47_19275 0.67 -1.0 82,788 - IAI47_19275 0.67 -2.6 82,831 + IAI47_19275 0.75 -0.9 82,832 - IAI47_19275 0.76 +0.7 82,913 - -0.1 82,913 - +0.1 82,920 + +0.7 82,921 - +0.6 82,958 + -1.4 82,958 + +1.2 82,958 + -1.6 82,958 + -1.8 82,958 + +1.0 82,958 + +0.0 82,958 + +1.3 82,959 - -1.1 82,959 - +1.0 82,959 - +1.0 82,959 - +0.6 82,962 + -0.3 82,962 + +1.2 82,962 + -0.8 82,962 + -0.8 82,962 + -1.0 82,962 + +0.0 82,962 + -0.8 82,963 - +0.8 82,963 - +0.7 82,963 - +2.0 82,963 - +0.6 82,963 - +0.3 83,005 + +1.2 83,010 + +1.0 83,011 - -1.9 83,014 + +0.3 83,015 - -1.3 83,015 - -1.2 83,015 - -0.2 83,015 - -1.7 83,015 - +0.1 83,015 - +0.8 83,015 - +1.6 83,098 + -0.9 83,099 - +0.6 83,101 + -1.2 83,101 + +1.6 83,101 + -0.4 83,101 + -0.8 83,101 + -1.0 83,102 - +2.6 83,102 - -0.3 83,102 - -0.8 83,102 - +3.2 83,102 - +0.5 83,102 - -1.5 83,198 + IAI47_19280 0.11 -1.3 83,205 + IAI47_19280 0.12 -1.1 83,206 - IAI47_19280 0.12 -1.1 83,206 - IAI47_19280 0.12 +0.9 83,235 + IAI47_19280 0.14 +0.7 83,236 - IAI47_19280 0.14 +0.2 83,236 - IAI47_19280 0.14 +0.8 83,236 - IAI47_19280 0.14 +0.1 83,268 + IAI47_19280 0.16 +2.3 83,275 + IAI47_19280 0.17 +0.3 83,275 + IAI47_19280 0.17 +2.0 83,276 - IAI47_19280 0.17 -0.2 83,344 + IAI47_19280 0.22 -1.5 83,345 - IAI47_19280 0.22 -0.1 83,345 - IAI47_19280 0.22 +0.7 83,432 + IAI47_19280 0.29 -0.3 83,432 + IAI47_19280 0.29 +0.5 83,432 + IAI47_19280 0.29 +3.2 83,432 + IAI47_19280 0.29 -0.8 83,432 + IAI47_19280 0.29 +0.3 83,433 - IAI47_19280 0.29 -0.4 83,433 - IAI47_19280 0.29 +0.8 83,433 - IAI47_19280 0.29 -1.0 83,433 - IAI47_19280 0.29 +0.2 83,433 - IAI47_19280 0.29 +0.9 83,433 - IAI47_19280 0.29 -0.1 83,500 - IAI47_19280 0.34 -2.0 83,500 - IAI47_19280 0.34 -1.8 83,520 + IAI47_19280 0.36 +0.2 83,520 + IAI47_19280 0.36 +2.2 83,537 - IAI47_19280 0.37 -0.6 83,540 + IAI47_19280 0.38 -2.0 83,541 + IAI47_19280 0.38 +0.5 83,541 + IAI47_19280 0.38 +1.2 83,541 + IAI47_19280 0.38 +0.8 83,541 + IAI47_19280 0.38 -0.6 83,541 + IAI47_19280 0.38 -1.6 83,541 + IAI47_19280 0.38 -1.5 83,541 + IAI47_19280 0.38 -0.0 83,542 - IAI47_19280 0.38 +2.0 83,542 - IAI47_19280 0.38 -1.6 83,542 - IAI47_19280 0.38 -0.3 83,542 - IAI47_19280 0.38 -0.1 83,542 - IAI47_19280 0.38 +3.2 83,542 - IAI47_19280 0.38 +3.8 83,542 - IAI47_19280 0.38 +2.9 83,542 - IAI47_19280 0.38 -0.1 83,542 - IAI47_19280 0.38 +1.1 83,565 + IAI47_19280 0.40 -0.2 83,566 - IAI47_19280 0.40 -0.5 83,566 - IAI47_19280 0.40 -0.1 83,566 - IAI47_19280 0.40 -0.5 83,566 - IAI47_19280 0.40 -0.6 83,581 - IAI47_19280 0.41 +1.3 83,656 - IAI47_19280 0.47 +0.1 83,753 + IAI47_19280 0.54 +1.2 83,753 + IAI47_19280 0.54 +0.1 83,753 + IAI47_19280 0.54 -0.9 83,754 - IAI47_19280 0.54 +0.2 83,754 - IAI47_19280 0.54 -0.8 83,754 - IAI47_19280 0.54 +0.2 83,754 - IAI47_19280 0.54 -0.6 83,754 - IAI47_19280 0.54 -0.5 83,754 - IAI47_19280 0.54 +1.8 83,754 - IAI47_19280 0.54 -0.7 83,754 - IAI47_19280 0.54 +3.2 83,765 + IAI47_19280 0.55 -2.1 83,765 + IAI47_19280 0.55 +0.6 83,765 + IAI47_19280 0.55 -0.0 83,765 + IAI47_19280 0.55 +0.6 83,765 + IAI47_19280 0.55 +0.5 83,765 + IAI47_19280 0.55 +2.7 83,765 + IAI47_19280 0.55 -1.1 83,765 + IAI47_19280 0.55 +0.6 83,765 + IAI47_19280 0.55 +0.2 83,766 - IAI47_19280 0.55 +2.1 83,766 - IAI47_19280 0.55 -0.4 83,766 - IAI47_19280 0.55 -0.9 83,766 - IAI47_19280 0.55 +1.6 83,766 - IAI47_19280 0.55 -0.6 83,766 - IAI47_19280 0.55 -0.8 83,766 - IAI47_19280 0.55 +1.2 83,766 - IAI47_19280 0.55 +1.2 83,766 - IAI47_19280 0.55 -0.8 83,766 - IAI47_19280 0.55 +0.6 83,767 + IAI47_19280 0.55 +0.5 83,767 + IAI47_19280 0.55 -0.5 83,767 + IAI47_19280 0.55 +0.3 83,767 + IAI47_19280 0.55 -0.6 83,768 - IAI47_19280 0.55 -0.5 83,768 - IAI47_19280 0.55 +0.5 83,768 - IAI47_19280 0.55 -2.3 83,768 - IAI47_19280 0.55 +0.7 83,768 - IAI47_19280 0.55 -0.4 83,768 - IAI47_19280 0.55 +0.9 83,768 - IAI47_19280 0.55 -0.6 83,768 - IAI47_19280 0.55 -1.3 83,768 - IAI47_19280 0.55 -0.5 83,790 + IAI47_19280 0.57 +0.0 83,851 + IAI47_19280 0.62 -0.5 83,852 - IAI47_19280 0.62 +2.2 83,852 - IAI47_19280 0.62 +0.5 83,935 - IAI47_19280 0.68 -0.2 83,935 - IAI47_19280 0.68 -1.8 83,960 + IAI47_19280 0.70 -1.1 83,960 + IAI47_19280 0.70 +0.4
Or see this region's nucleotide sequence