Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_15035 and IAI47_15040 are separated by 87 nucleotides IAI47_15040 and IAI47_15045 are separated by 90 nucleotides
IAI47_15035: IAI47_15035 - cyanate transporter, at 3,170,767 to 3,171,939
_15035
IAI47_15040: IAI47_15040 - LysR family transcriptional regulator, at 3,172,027 to 3,172,887
_15040
IAI47_15045: IAI47_15045 - amidohydrolase family protein, at 3,172,978 to 3,174,366
_15045
Position (kb)
3172
3173 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3171.210 kb on + strand, within IAI47_15035 at 3171.210 kb on + strand, within IAI47_15035 at 3171.210 kb on + strand, within IAI47_15035 at 3171.210 kb on + strand, within IAI47_15035 at 3171.211 kb on - strand, within IAI47_15035 at 3171.257 kb on + strand, within IAI47_15035 at 3171.290 kb on + strand, within IAI47_15035 at 3171.290 kb on + strand, within IAI47_15035 at 3171.290 kb on + strand, within IAI47_15035 at 3171.290 kb on + strand, within IAI47_15035 at 3171.290 kb on + strand, within IAI47_15035 at 3171.291 kb on - strand, within IAI47_15035 at 3171.291 kb on - strand, within IAI47_15035 at 3171.291 kb on - strand, within IAI47_15035 at 3171.402 kb on + strand, within IAI47_15035 at 3171.402 kb on + strand, within IAI47_15035 at 3171.420 kb on + strand, within IAI47_15035 at 3171.421 kb on - strand, within IAI47_15035 at 3171.447 kb on + strand, within IAI47_15035 at 3171.448 kb on - strand, within IAI47_15035 at 3171.448 kb on - strand, within IAI47_15035 at 3171.448 kb on - strand, within IAI47_15035 at 3171.604 kb on + strand, within IAI47_15035 at 3171.604 kb on + strand, within IAI47_15035 at 3171.604 kb on + strand, within IAI47_15035 at 3171.604 kb on + strand, within IAI47_15035 at 3171.604 kb on + strand, within IAI47_15035 at 3171.604 kb on + strand, within IAI47_15035 at 3171.605 kb on - strand, within IAI47_15035 at 3171.605 kb on - strand, within IAI47_15035 at 3171.605 kb on - strand, within IAI47_15035 at 3171.605 kb on - strand, within IAI47_15035 at 3171.605 kb on - strand, within IAI47_15035 at 3171.663 kb on + strand, within IAI47_15035 at 3171.761 kb on - strand, within IAI47_15035 at 3171.799 kb on + strand, within IAI47_15035 at 3171.799 kb on + strand, within IAI47_15035 at 3171.800 kb on - strand, within IAI47_15035 at 3171.800 kb on - strand, within IAI47_15035 at 3171.800 kb on - strand, within IAI47_15035 at 3171.885 kb on + strand at 3171.885 kb on + strand at 3171.886 kb on - strand at 3171.886 kb on - strand at 3171.886 kb on - strand at 3171.886 kb on - strand at 3171.956 kb on + strand at 3171.957 kb on - strand at 3171.985 kb on - strand at 3171.987 kb on + strand at 3172.002 kb on + strand at 3172.002 kb on + strand at 3172.002 kb on + strand at 3172.003 kb on - strand at 3172.047 kb on + strand at 3172.047 kb on + strand at 3172.066 kb on - strand at 3172.066 kb on - strand at 3172.066 kb on - strand at 3172.066 kb on - strand at 3172.066 kb on - strand at 3172.172 kb on + strand, within IAI47_15040 at 3172.172 kb on + strand, within IAI47_15040 at 3172.173 kb on - strand, within IAI47_15040 at 3172.238 kb on + strand, within IAI47_15040 at 3172.244 kb on - strand, within IAI47_15040 at 3172.257 kb on + strand, within IAI47_15040 at 3172.258 kb on - strand, within IAI47_15040 at 3172.258 kb on - strand, within IAI47_15040 at 3172.258 kb on - strand, within IAI47_15040 at 3172.258 kb on - strand, within IAI47_15040 at 3172.653 kb on + strand, within IAI47_15040 at 3172.653 kb on + strand, within IAI47_15040 at 3172.653 kb on + strand, within IAI47_15040 at 3172.653 kb on + strand, within IAI47_15040 at 3172.653 kb on + strand, within IAI47_15040 at 3172.653 kb on + strand, within IAI47_15040 at 3172.653 kb on + strand, within IAI47_15040 at 3172.654 kb on - strand, within IAI47_15040 at 3172.654 kb on - strand, within IAI47_15040 at 3172.654 kb on - strand, within IAI47_15040 at 3172.657 kb on + strand, within IAI47_15040 at 3172.657 kb on + strand, within IAI47_15040 at 3172.657 kb on + strand, within IAI47_15040 at 3172.657 kb on + strand, within IAI47_15040 at 3172.658 kb on - strand, within IAI47_15040 at 3172.719 kb on - strand, within IAI47_15040 at 3172.719 kb on - strand, within IAI47_15040 at 3172.789 kb on - strand, within IAI47_15040 at 3172.789 kb on - strand, within IAI47_15040 at 3172.890 kb on + strand at 3172.891 kb on - strand at 3172.897 kb on + strand at 3172.898 kb on - strand at 3172.898 kb on - strand at 3172.898 kb on - strand at 3172.898 kb on - strand at 3172.898 kb on - strand at 3172.914 kb on + strand at 3172.915 kb on - strand at 3172.915 kb on - strand at 3172.947 kb on + strand at 3172.947 kb on + strand at 3172.947 kb on + strand at 3172.948 kb on - strand at 3172.948 kb on - strand at 3172.983 kb on + strand at 3172.983 kb on + strand at 3172.983 kb on + strand at 3173.051 kb on + strand at 3173.051 kb on + strand at 3173.051 kb on + strand at 3173.051 kb on + strand at 3173.149 kb on + strand, within IAI47_15045 at 3173.149 kb on + strand, within IAI47_15045 at 3173.149 kb on + strand, within IAI47_15045 at 3173.149 kb on + strand, within IAI47_15045 at 3173.150 kb on - strand, within IAI47_15045 at 3173.185 kb on - strand, within IAI47_15045 at 3173.227 kb on + strand, within IAI47_15045 at 3173.227 kb on + strand, within IAI47_15045 at 3173.343 kb on + strand, within IAI47_15045 at 3173.343 kb on + strand, within IAI47_15045 at 3173.361 kb on + strand, within IAI47_15045 at 3173.361 kb on + strand, within IAI47_15045 at 3173.362 kb on - strand, within IAI47_15045 at 3173.371 kb on + strand, within IAI47_15045 at 3173.371 kb on + strand, within IAI47_15045 at 3173.371 kb on + strand, within IAI47_15045 at 3173.371 kb on + strand, within IAI47_15045 at 3173.371 kb on + strand, within IAI47_15045 at 3173.372 kb on - strand, within IAI47_15045 at 3173.372 kb on - strand, within IAI47_15045 at 3173.372 kb on - strand, within IAI47_15045 at 3173.424 kb on + strand, within IAI47_15045 at 3173.424 kb on + strand, within IAI47_15045 at 3173.424 kb on + strand, within IAI47_15045 at 3173.424 kb on + strand, within IAI47_15045 at 3173.425 kb on - strand, within IAI47_15045 at 3173.425 kb on - strand, within IAI47_15045 at 3173.425 kb on - strand, within IAI47_15045 at 3173.425 kb on - strand, within IAI47_15045 at 3173.565 kb on + strand, within IAI47_15045 at 3173.565 kb on + strand, within IAI47_15045 at 3173.566 kb on - strand, within IAI47_15045 at 3173.594 kb on + strand, within IAI47_15045 at 3173.699 kb on + strand, within IAI47_15045 at 3173.700 kb on - strand, within IAI47_15045 at 3173.700 kb on - strand, within IAI47_15045 at 3173.700 kb on - strand, within IAI47_15045 at 3173.704 kb on + strand, within IAI47_15045 at 3173.704 kb on + strand, within IAI47_15045 at 3173.704 kb on + strand, within IAI47_15045 at 3173.704 kb on + strand, within IAI47_15045 at 3173.704 kb on + strand, within IAI47_15045 at 3173.705 kb on - strand, within IAI47_15045 at 3173.705 kb on - strand, within IAI47_15045 at 3173.705 kb on - strand, within IAI47_15045 at 3173.705 kb on - strand, within IAI47_15045 at 3173.785 kb on + strand, within IAI47_15045 at 3173.785 kb on + strand, within IAI47_15045 at 3173.848 kb on + strand, within IAI47_15045 at 3173.848 kb on + strand, within IAI47_15045 at 3173.848 kb on + strand, within IAI47_15045 at 3173.848 kb on + strand, within IAI47_15045 at 3173.848 kb on + strand, within IAI47_15045 at 3173.848 kb on + strand, within IAI47_15045 at 3173.848 kb on + strand, within IAI47_15045 at 3173.849 kb on - strand, within IAI47_15045 at 3173.849 kb on - strand, within IAI47_15045 at 3173.849 kb on - strand, within IAI47_15045 at 3173.882 kb on + strand, within IAI47_15045 at 3173.882 kb on + strand, within IAI47_15045 at 3173.882 kb on + strand, within IAI47_15045 at 3173.883 kb on - strand, within IAI47_15045
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 3,171,210 + IAI47_15035 0.38 -1.8 3,171,210 + IAI47_15035 0.38 +0.2 3,171,210 + IAI47_15035 0.38 +0.0 3,171,210 + IAI47_15035 0.38 -0.4 3,171,211 - IAI47_15035 0.38 -0.6 3,171,257 + IAI47_15035 0.42 +2.7 3,171,290 + IAI47_15035 0.45 +0.0 3,171,290 + IAI47_15035 0.45 -0.7 3,171,290 + IAI47_15035 0.45 -1.4 3,171,290 + IAI47_15035 0.45 -1.0 3,171,290 + IAI47_15035 0.45 -0.7 3,171,291 - IAI47_15035 0.45 -0.8 3,171,291 - IAI47_15035 0.45 -0.1 3,171,291 - IAI47_15035 0.45 -0.4 3,171,402 + IAI47_15035 0.54 -0.6 3,171,402 + IAI47_15035 0.54 -1.3 3,171,420 + IAI47_15035 0.56 -2.1 3,171,421 - IAI47_15035 0.56 -0.4 3,171,447 + IAI47_15035 0.58 -2.2 3,171,448 - IAI47_15035 0.58 -0.2 3,171,448 - IAI47_15035 0.58 -1.3 3,171,448 - IAI47_15035 0.58 -0.1 3,171,604 + IAI47_15035 0.71 -1.0 3,171,604 + IAI47_15035 0.71 -0.3 3,171,604 + IAI47_15035 0.71 +0.9 3,171,604 + IAI47_15035 0.71 -1.7 3,171,604 + IAI47_15035 0.71 +1.0 3,171,604 + IAI47_15035 0.71 +0.8 3,171,605 - IAI47_15035 0.71 -0.1 3,171,605 - IAI47_15035 0.71 +0.7 3,171,605 - IAI47_15035 0.71 -0.1 3,171,605 - IAI47_15035 0.71 -0.8 3,171,605 - IAI47_15035 0.71 +1.9 3,171,663 + IAI47_15035 0.76 -0.3 3,171,761 - IAI47_15035 0.85 +1.0 3,171,799 + IAI47_15035 0.88 -0.1 3,171,799 + IAI47_15035 0.88 -0.5 3,171,800 - IAI47_15035 0.88 -0.0 3,171,800 - IAI47_15035 0.88 -2.1 3,171,800 - IAI47_15035 0.88 -1.0 3,171,885 + -1.0 3,171,885 + -0.4 3,171,886 - -1.0 3,171,886 - +0.1 3,171,886 - -0.9 3,171,886 - +2.9 3,171,956 + +3.3 3,171,957 - +2.4 3,171,985 - +0.2 3,171,987 + +1.5 3,172,002 + +1.8 3,172,002 + +0.3 3,172,002 + +1.2 3,172,003 - +1.1 3,172,047 + -0.6 3,172,047 + -1.3 3,172,066 - +1.0 3,172,066 - -1.0 3,172,066 - +0.7 3,172,066 - +0.1 3,172,066 - +0.6 3,172,172 + IAI47_15040 0.17 -1.2 3,172,172 + IAI47_15040 0.17 -0.1 3,172,173 - IAI47_15040 0.17 +0.4 3,172,238 + IAI47_15040 0.25 -0.6 3,172,244 - IAI47_15040 0.25 +1.9 3,172,257 + IAI47_15040 0.27 -1.3 3,172,258 - IAI47_15040 0.27 +1.4 3,172,258 - IAI47_15040 0.27 +1.1 3,172,258 - IAI47_15040 0.27 -1.1 3,172,258 - IAI47_15040 0.27 +2.0 3,172,653 + IAI47_15040 0.73 +0.9 3,172,653 + IAI47_15040 0.73 +2.6 3,172,653 + IAI47_15040 0.73 +1.0 3,172,653 + IAI47_15040 0.73 -0.1 3,172,653 + IAI47_15040 0.73 +0.3 3,172,653 + IAI47_15040 0.73 +0.3 3,172,653 + IAI47_15040 0.73 +2.0 3,172,654 - IAI47_15040 0.73 +0.1 3,172,654 - IAI47_15040 0.73 -0.0 3,172,654 - IAI47_15040 0.73 +0.7 3,172,657 + IAI47_15040 0.73 +0.4 3,172,657 + IAI47_15040 0.73 -0.1 3,172,657 + IAI47_15040 0.73 +0.4 3,172,657 + IAI47_15040 0.73 -0.4 3,172,658 - IAI47_15040 0.73 +0.3 3,172,719 - IAI47_15040 0.80 -1.0 3,172,719 - IAI47_15040 0.80 +0.3 3,172,789 - IAI47_15040 0.89 +2.0 3,172,789 - IAI47_15040 0.89 +0.9 3,172,890 + -1.6 3,172,891 - +1.7 3,172,897 + -0.7 3,172,898 - +2.1 3,172,898 - -0.6 3,172,898 - +0.0 3,172,898 - -1.7 3,172,898 - -0.4 3,172,914 + -0.8 3,172,915 - -1.6 3,172,915 - +1.4 3,172,947 + -0.6 3,172,947 + +0.0 3,172,947 + +2.1 3,172,948 - -0.1 3,172,948 - -0.8 3,172,983 + -0.3 3,172,983 + +1.3 3,172,983 + -0.1 3,173,051 + +3.4 3,173,051 + -0.4 3,173,051 + +0.2 3,173,051 + +1.8 3,173,149 + IAI47_15045 0.12 -1.3 3,173,149 + IAI47_15045 0.12 +0.6 3,173,149 + IAI47_15045 0.12 +1.2 3,173,149 + IAI47_15045 0.12 -2.0 3,173,150 - IAI47_15045 0.12 -0.6 3,173,185 - IAI47_15045 0.15 +0.3 3,173,227 + IAI47_15045 0.18 +0.8 3,173,227 + IAI47_15045 0.18 +1.0 3,173,343 + IAI47_15045 0.26 +0.9 3,173,343 + IAI47_15045 0.26 +1.9 3,173,361 + IAI47_15045 0.28 -0.3 3,173,361 + IAI47_15045 0.28 -0.0 3,173,362 - IAI47_15045 0.28 +0.4 3,173,371 + IAI47_15045 0.28 -0.1 3,173,371 + IAI47_15045 0.28 -1.0 3,173,371 + IAI47_15045 0.28 +1.5 3,173,371 + IAI47_15045 0.28 +2.0 3,173,371 + IAI47_15045 0.28 -0.5 3,173,372 - IAI47_15045 0.28 +2.5 3,173,372 - IAI47_15045 0.28 +0.5 3,173,372 - IAI47_15045 0.28 +1.7 3,173,424 + IAI47_15045 0.32 -0.0 3,173,424 + IAI47_15045 0.32 +0.1 3,173,424 + IAI47_15045 0.32 -0.2 3,173,424 + IAI47_15045 0.32 -0.7 3,173,425 - IAI47_15045 0.32 -1.5 3,173,425 - IAI47_15045 0.32 +0.1 3,173,425 - IAI47_15045 0.32 -0.1 3,173,425 - IAI47_15045 0.32 -0.1 3,173,565 + IAI47_15045 0.42 +0.0 3,173,565 + IAI47_15045 0.42 -0.7 3,173,566 - IAI47_15045 0.42 -0.0 3,173,594 + IAI47_15045 0.44 -1.5 3,173,699 + IAI47_15045 0.52 -0.9 3,173,700 - IAI47_15045 0.52 -0.5 3,173,700 - IAI47_15045 0.52 -0.1 3,173,700 - IAI47_15045 0.52 -1.5 3,173,704 + IAI47_15045 0.52 +0.2 3,173,704 + IAI47_15045 0.52 +0.0 3,173,704 + IAI47_15045 0.52 +1.0 3,173,704 + IAI47_15045 0.52 +1.2 3,173,704 + IAI47_15045 0.52 -1.9 3,173,705 - IAI47_15045 0.52 +2.1 3,173,705 - IAI47_15045 0.52 -1.1 3,173,705 - IAI47_15045 0.52 -0.1 3,173,705 - IAI47_15045 0.52 +0.3 3,173,785 + IAI47_15045 0.58 +0.0 3,173,785 + IAI47_15045 0.58 -1.0 3,173,848 + IAI47_15045 0.63 -0.8 3,173,848 + IAI47_15045 0.63 -0.5 3,173,848 + IAI47_15045 0.63 +0.3 3,173,848 + IAI47_15045 0.63 -0.6 3,173,848 + IAI47_15045 0.63 -0.3 3,173,848 + IAI47_15045 0.63 -1.3 3,173,848 + IAI47_15045 0.63 -0.2 3,173,849 - IAI47_15045 0.63 +1.9 3,173,849 - IAI47_15045 0.63 +0.4 3,173,849 - IAI47_15045 0.63 -0.1 3,173,882 + IAI47_15045 0.65 -0.6 3,173,882 + IAI47_15045 0.65 +0.6 3,173,882 + IAI47_15045 0.65 +0.4 3,173,883 - IAI47_15045 0.65 +0.3
Or see this region's nucleotide sequence