Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_04185 and IAI47_04190 overlap by 1 nucleotides IAI47_04190 and IAI47_04195 are separated by 54 nucleotides IAI47_04195 and IAI47_04200 are separated by 169 nucleotides IAI47_04200 and IAI47_04205 are separated by 3 nucleotides
IAI47_04185: IAI47_04185 - sulfate adenylyltransferase subunit CysN, at 880,261 to 881,688
_04185
IAI47_04190: IAI47_04190 - adenylyl-sulfate kinase, at 881,688 to 882,293
_04190
IAI47_04195: IAI47_04195 - DUF3561 family protein, at 882,348 to 882,668
_04195
IAI47_04200: IAI47_04200 - cell division protein FtsB, at 882,838 to 883,155
_04200
IAI47_04205: IAI47_04205 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 883,159 to 883,875
_04205
Position (kb)
881
882
883 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 880.719 kb on + strand, within IAI47_04185 at 880.719 kb on + strand, within IAI47_04185 at 880.720 kb on - strand, within IAI47_04185 at 880.720 kb on - strand, within IAI47_04185 at 880.720 kb on - strand, within IAI47_04185 at 880.761 kb on + strand, within IAI47_04185 at 880.761 kb on + strand, within IAI47_04185 at 880.762 kb on - strand, within IAI47_04185 at 880.764 kb on + strand, within IAI47_04185 at 880.764 kb on + strand, within IAI47_04185 at 880.764 kb on + strand, within IAI47_04185 at 880.764 kb on + strand, within IAI47_04185 at 880.765 kb on - strand, within IAI47_04185 at 880.765 kb on - strand, within IAI47_04185 at 880.765 kb on - strand, within IAI47_04185 at 880.765 kb on - strand, within IAI47_04185 at 880.765 kb on - strand, within IAI47_04185 at 880.783 kb on + strand, within IAI47_04185 at 880.784 kb on - strand, within IAI47_04185 at 880.784 kb on - strand, within IAI47_04185 at 880.798 kb on + strand, within IAI47_04185 at 880.799 kb on - strand, within IAI47_04185 at 880.799 kb on - strand, within IAI47_04185 at 880.819 kb on + strand, within IAI47_04185 at 880.820 kb on - strand, within IAI47_04185 at 880.820 kb on - strand, within IAI47_04185 at 880.820 kb on - strand, within IAI47_04185 at 880.820 kb on - strand, within IAI47_04185 at 880.820 kb on - strand, within IAI47_04185 at 880.820 kb on - strand, within IAI47_04185 at 880.927 kb on + strand, within IAI47_04185 at 880.928 kb on - strand, within IAI47_04185 at 880.928 kb on - strand, within IAI47_04185 at 880.928 kb on - strand, within IAI47_04185 at 880.928 kb on - strand, within IAI47_04185 at 881.014 kb on + strand, within IAI47_04185 at 881.014 kb on + strand, within IAI47_04185 at 881.014 kb on + strand, within IAI47_04185 at 881.014 kb on + strand, within IAI47_04185 at 881.014 kb on + strand, within IAI47_04185 at 881.014 kb on + strand, within IAI47_04185 at 881.014 kb on + strand, within IAI47_04185 at 881.014 kb on + strand, within IAI47_04185 at 881.014 kb on + strand, within IAI47_04185 at 881.015 kb on - strand, within IAI47_04185 at 881.015 kb on - strand, within IAI47_04185 at 881.015 kb on - strand, within IAI47_04185 at 881.015 kb on - strand, within IAI47_04185 at 881.015 kb on - strand, within IAI47_04185 at 881.015 kb on - strand, within IAI47_04185 at 881.015 kb on - strand, within IAI47_04185 at 881.015 kb on - strand, within IAI47_04185 at 881.015 kb on - strand, within IAI47_04185 at 881.019 kb on + strand, within IAI47_04185 at 881.020 kb on - strand, within IAI47_04185 at 881.028 kb on + strand, within IAI47_04185 at 881.028 kb on + strand, within IAI47_04185 at 881.028 kb on + strand, within IAI47_04185 at 881.047 kb on + strand, within IAI47_04185 at 881.047 kb on + strand, within IAI47_04185 at 881.048 kb on - strand, within IAI47_04185 at 881.114 kb on + strand, within IAI47_04185 at 881.115 kb on - strand, within IAI47_04185 at 881.125 kb on - strand, within IAI47_04185 at 881.125 kb on - strand, within IAI47_04185 at 881.135 kb on + strand, within IAI47_04185 at 881.135 kb on + strand, within IAI47_04185 at 881.135 kb on + strand, within IAI47_04185 at 881.136 kb on - strand, within IAI47_04185 at 881.136 kb on - strand, within IAI47_04185 at 881.136 kb on - strand, within IAI47_04185 at 881.209 kb on + strand, within IAI47_04185 at 881.211 kb on + strand, within IAI47_04185 at 881.211 kb on + strand, within IAI47_04185 at 881.211 kb on + strand, within IAI47_04185 at 881.211 kb on + strand, within IAI47_04185 at 881.212 kb on - strand, within IAI47_04185 at 881.323 kb on + strand, within IAI47_04185 at 881.323 kb on + strand, within IAI47_04185 at 881.324 kb on - strand, within IAI47_04185 at 881.324 kb on - strand, within IAI47_04185 at 881.324 kb on - strand, within IAI47_04185 at 881.324 kb on - strand, within IAI47_04185 at 881.324 kb on - strand, within IAI47_04185 at 881.391 kb on + strand, within IAI47_04185 at 881.391 kb on + strand, within IAI47_04185 at 881.391 kb on + strand, within IAI47_04185 at 881.436 kb on + strand, within IAI47_04185 at 881.453 kb on + strand, within IAI47_04185 at 881.454 kb on - strand, within IAI47_04185 at 881.485 kb on + strand, within IAI47_04185 at 881.485 kb on + strand, within IAI47_04185 at 881.485 kb on + strand, within IAI47_04185 at 881.485 kb on + strand, within IAI47_04185 at 881.486 kb on - strand, within IAI47_04185 at 881.486 kb on - strand, within IAI47_04185 at 881.486 kb on - strand, within IAI47_04185 at 881.486 kb on - strand, within IAI47_04185 at 881.486 kb on - strand, within IAI47_04185 at 881.486 kb on - strand, within IAI47_04185 at 881.520 kb on + strand, within IAI47_04185 at 881.589 kb on + strand at 881.592 kb on + strand at 881.592 kb on + strand at 881.687 kb on - strand at 881.705 kb on + strand at 881.705 kb on + strand at 881.705 kb on + strand at 881.705 kb on + strand at 881.706 kb on - strand at 881.706 kb on - strand at 881.706 kb on - strand at 881.706 kb on - strand at 881.706 kb on - strand at 881.731 kb on + strand at 881.731 kb on + strand at 881.849 kb on + strand, within IAI47_04190 at 881.850 kb on - strand, within IAI47_04190 at 881.861 kb on + strand, within IAI47_04190 at 881.861 kb on + strand, within IAI47_04190 at 881.862 kb on - strand, within IAI47_04190 at 881.862 kb on - strand, within IAI47_04190 at 881.862 kb on - strand, within IAI47_04190 at 881.862 kb on - strand, within IAI47_04190 at 881.862 kb on - strand, within IAI47_04190 at 881.865 kb on + strand, within IAI47_04190 at 881.865 kb on + strand, within IAI47_04190 at 881.865 kb on + strand, within IAI47_04190 at 881.866 kb on - strand, within IAI47_04190 at 881.866 kb on - strand, within IAI47_04190 at 881.866 kb on - strand, within IAI47_04190 at 882.008 kb on + strand, within IAI47_04190 at 882.127 kb on + strand, within IAI47_04190 at 882.127 kb on + strand, within IAI47_04190 at 882.128 kb on - strand, within IAI47_04190 at 882.129 kb on + strand, within IAI47_04190 at 882.129 kb on + strand, within IAI47_04190 at 882.129 kb on + strand, within IAI47_04190 at 882.130 kb on - strand, within IAI47_04190 at 882.130 kb on - strand, within IAI47_04190 at 882.158 kb on + strand, within IAI47_04190 at 882.159 kb on - strand, within IAI47_04190 at 882.183 kb on + strand, within IAI47_04190 at 882.184 kb on - strand, within IAI47_04190 at 882.233 kb on + strand at 882.233 kb on + strand at 882.234 kb on - strand at 882.256 kb on + strand at 882.256 kb on + strand at 882.256 kb on + strand at 882.278 kb on + strand at 882.278 kb on + strand at 882.347 kb on + strand at 882.347 kb on + strand at 882.347 kb on + strand at 882.347 kb on + strand at 882.347 kb on + strand at 882.348 kb on - strand at 882.348 kb on - strand at 882.348 kb on - strand at 882.348 kb on - strand at 882.348 kb on - strand at 882.348 kb on - strand at 882.377 kb on + strand at 882.377 kb on + strand at 882.378 kb on - strand at 882.436 kb on + strand, within IAI47_04195 at 882.436 kb on + strand, within IAI47_04195 at 882.437 kb on - strand, within IAI47_04195 at 882.444 kb on + strand, within IAI47_04195 at 882.444 kb on + strand, within IAI47_04195 at 882.445 kb on - strand, within IAI47_04195 at 882.507 kb on + strand, within IAI47_04195 at 882.507 kb on + strand, within IAI47_04195 at 882.507 kb on + strand, within IAI47_04195 at 882.507 kb on + strand, within IAI47_04195 at 882.509 kb on + strand, within IAI47_04195 at 882.510 kb on - strand, within IAI47_04195 at 882.569 kb on + strand, within IAI47_04195 at 882.569 kb on + strand, within IAI47_04195 at 882.570 kb on - strand, within IAI47_04195 at 882.608 kb on + strand, within IAI47_04195 at 882.608 kb on + strand, within IAI47_04195 at 882.608 kb on + strand, within IAI47_04195 at 882.608 kb on + strand, within IAI47_04195 at 882.609 kb on - strand, within IAI47_04195 at 882.617 kb on + strand, within IAI47_04195 at 882.617 kb on + strand, within IAI47_04195 at 882.617 kb on + strand, within IAI47_04195 at 882.618 kb on - strand, within IAI47_04195 at 882.618 kb on - strand, within IAI47_04195 at 882.618 kb on - strand, within IAI47_04195 at 882.640 kb on + strand at 882.640 kb on + strand at 882.641 kb on - strand at 882.641 kb on - strand at 882.667 kb on - strand at 882.712 kb on + strand at 882.738 kb on + strand at 883.145 kb on + strand at 883.153 kb on + strand at 883.153 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 880,719 + IAI47_04185 0.32 +0.5 880,719 + IAI47_04185 0.32 +0.8 880,720 - IAI47_04185 0.32 +0.1 880,720 - IAI47_04185 0.32 +1.4 880,720 - IAI47_04185 0.32 -1.6 880,761 + IAI47_04185 0.35 -0.7 880,761 + IAI47_04185 0.35 +0.9 880,762 - IAI47_04185 0.35 +1.0 880,764 + IAI47_04185 0.35 -1.1 880,764 + IAI47_04185 0.35 +2.4 880,764 + IAI47_04185 0.35 +1.1 880,764 + IAI47_04185 0.35 +1.1 880,765 - IAI47_04185 0.35 +1.2 880,765 - IAI47_04185 0.35 -0.5 880,765 - IAI47_04185 0.35 -1.0 880,765 - IAI47_04185 0.35 +1.3 880,765 - IAI47_04185 0.35 -1.4 880,783 + IAI47_04185 0.37 +3.1 880,784 - IAI47_04185 0.37 -1.0 880,784 - IAI47_04185 0.37 -0.4 880,798 + IAI47_04185 0.38 -0.2 880,799 - IAI47_04185 0.38 +0.8 880,799 - IAI47_04185 0.38 +0.4 880,819 + IAI47_04185 0.39 +1.5 880,820 - IAI47_04185 0.39 +0.2 880,820 - IAI47_04185 0.39 -0.6 880,820 - IAI47_04185 0.39 -0.8 880,820 - IAI47_04185 0.39 +1.0 880,820 - IAI47_04185 0.39 -0.9 880,820 - IAI47_04185 0.39 +1.1 880,927 + IAI47_04185 0.47 -0.5 880,928 - IAI47_04185 0.47 -0.5 880,928 - IAI47_04185 0.47 +1.4 880,928 - IAI47_04185 0.47 -1.7 880,928 - IAI47_04185 0.47 +0.5 881,014 + IAI47_04185 0.53 +0.4 881,014 + IAI47_04185 0.53 -0.2 881,014 + IAI47_04185 0.53 -1.9 881,014 + IAI47_04185 0.53 -0.0 881,014 + IAI47_04185 0.53 +1.4 881,014 + IAI47_04185 0.53 -0.6 881,014 + IAI47_04185 0.53 +0.6 881,014 + IAI47_04185 0.53 -0.5 881,014 + IAI47_04185 0.53 +0.3 881,015 - IAI47_04185 0.53 +0.4 881,015 - IAI47_04185 0.53 -0.4 881,015 - IAI47_04185 0.53 +1.1 881,015 - IAI47_04185 0.53 -0.1 881,015 - IAI47_04185 0.53 -0.8 881,015 - IAI47_04185 0.53 +0.6 881,015 - IAI47_04185 0.53 +1.0 881,015 - IAI47_04185 0.53 -0.2 881,015 - IAI47_04185 0.53 -0.2 881,019 + IAI47_04185 0.53 -0.1 881,020 - IAI47_04185 0.53 +0.3 881,028 + IAI47_04185 0.54 -0.1 881,028 + IAI47_04185 0.54 +1.6 881,028 + IAI47_04185 0.54 -1.3 881,047 + IAI47_04185 0.55 -0.8 881,047 + IAI47_04185 0.55 -1.5 881,048 - IAI47_04185 0.55 +1.3 881,114 + IAI47_04185 0.60 -2.6 881,115 - IAI47_04185 0.60 +0.7 881,125 - IAI47_04185 0.61 -0.8 881,125 - IAI47_04185 0.61 +1.6 881,135 + IAI47_04185 0.61 +1.6 881,135 + IAI47_04185 0.61 +1.1 881,135 + IAI47_04185 0.61 +3.4 881,136 - IAI47_04185 0.61 +0.1 881,136 - IAI47_04185 0.61 -0.9 881,136 - IAI47_04185 0.61 -0.8 881,209 + IAI47_04185 0.66 -0.3 881,211 + IAI47_04185 0.67 +0.2 881,211 + IAI47_04185 0.67 +0.3 881,211 + IAI47_04185 0.67 +0.1 881,211 + IAI47_04185 0.67 +1.9 881,212 - IAI47_04185 0.67 -1.2 881,323 + IAI47_04185 0.74 +1.4 881,323 + IAI47_04185 0.74 -1.2 881,324 - IAI47_04185 0.74 +0.4 881,324 - IAI47_04185 0.74 +2.2 881,324 - IAI47_04185 0.74 -0.7 881,324 - IAI47_04185 0.74 -0.4 881,324 - IAI47_04185 0.74 +1.8 881,391 + IAI47_04185 0.79 +0.4 881,391 + IAI47_04185 0.79 +2.0 881,391 + IAI47_04185 0.79 +0.1 881,436 + IAI47_04185 0.82 -1.0 881,453 + IAI47_04185 0.83 -0.8 881,454 - IAI47_04185 0.84 -1.0 881,485 + IAI47_04185 0.86 +0.3 881,485 + IAI47_04185 0.86 +1.0 881,485 + IAI47_04185 0.86 -0.5 881,485 + IAI47_04185 0.86 -1.9 881,486 - IAI47_04185 0.86 -0.2 881,486 - IAI47_04185 0.86 -1.2 881,486 - IAI47_04185 0.86 -0.3 881,486 - IAI47_04185 0.86 +1.1 881,486 - IAI47_04185 0.86 +0.1 881,486 - IAI47_04185 0.86 +0.4 881,520 + IAI47_04185 0.88 +0.7 881,589 + +0.8 881,592 + +0.4 881,592 + +0.7 881,687 - +0.8 881,705 + +0.7 881,705 + -2.5 881,705 + +0.2 881,705 + +0.7 881,706 - -0.1 881,706 - -0.0 881,706 - -1.2 881,706 - +0.1 881,706 - +0.7 881,731 + -0.9 881,731 + -0.0 881,849 + IAI47_04190 0.27 -0.4 881,850 - IAI47_04190 0.27 +0.1 881,861 + IAI47_04190 0.29 +0.3 881,861 + IAI47_04190 0.29 -1.5 881,862 - IAI47_04190 0.29 -0.2 881,862 - IAI47_04190 0.29 +0.7 881,862 - IAI47_04190 0.29 -0.9 881,862 - IAI47_04190 0.29 +0.8 881,862 - IAI47_04190 0.29 -0.2 881,865 + IAI47_04190 0.29 -0.1 881,865 + IAI47_04190 0.29 +0.1 881,865 + IAI47_04190 0.29 -0.7 881,866 - IAI47_04190 0.29 -0.4 881,866 - IAI47_04190 0.29 -2.0 881,866 - IAI47_04190 0.29 -0.5 882,008 + IAI47_04190 0.53 -1.3 882,127 + IAI47_04190 0.72 +1.9 882,127 + IAI47_04190 0.72 -0.8 882,128 - IAI47_04190 0.73 -1.7 882,129 + IAI47_04190 0.73 +2.5 882,129 + IAI47_04190 0.73 -2.2 882,129 + IAI47_04190 0.73 +2.0 882,130 - IAI47_04190 0.73 -0.9 882,130 - IAI47_04190 0.73 +0.4 882,158 + IAI47_04190 0.78 -2.7 882,159 - IAI47_04190 0.78 -0.6 882,183 + IAI47_04190 0.82 +0.1 882,184 - IAI47_04190 0.82 -1.1 882,233 + +0.6 882,233 + +0.2 882,234 - +0.3 882,256 + -1.0 882,256 + -1.6 882,256 + -0.1 882,278 + -2.6 882,278 + +0.6 882,347 + +1.6 882,347 + -0.1 882,347 + -0.8 882,347 + +1.0 882,347 + -2.1 882,348 - -0.2 882,348 - -0.2 882,348 - -0.5 882,348 - +0.3 882,348 - +0.1 882,348 - +2.0 882,377 + -2.7 882,377 + +1.1 882,378 - -0.5 882,436 + IAI47_04195 0.27 -0.2 882,436 + IAI47_04195 0.27 +0.4 882,437 - IAI47_04195 0.28 -0.3 882,444 + IAI47_04195 0.30 +0.6 882,444 + IAI47_04195 0.30 +1.1 882,445 - IAI47_04195 0.30 -0.5 882,507 + IAI47_04195 0.50 -1.4 882,507 + IAI47_04195 0.50 +0.6 882,507 + IAI47_04195 0.50 +1.0 882,507 + IAI47_04195 0.50 +0.0 882,509 + IAI47_04195 0.50 +2.8 882,510 - IAI47_04195 0.50 +3.0 882,569 + IAI47_04195 0.69 -0.5 882,569 + IAI47_04195 0.69 +1.4 882,570 - IAI47_04195 0.69 -2.2 882,608 + IAI47_04195 0.81 -1.2 882,608 + IAI47_04195 0.81 +1.8 882,608 + IAI47_04195 0.81 +0.5 882,608 + IAI47_04195 0.81 +1.2 882,609 - IAI47_04195 0.81 -0.1 882,617 + IAI47_04195 0.84 +1.1 882,617 + IAI47_04195 0.84 -0.2 882,617 + IAI47_04195 0.84 +0.0 882,618 - IAI47_04195 0.84 -1.2 882,618 - IAI47_04195 0.84 +0.2 882,618 - IAI47_04195 0.84 -0.2 882,640 + -1.2 882,640 + +0.4 882,641 - +0.3 882,641 - -0.6 882,667 - -1.2 882,712 + -0.2 882,738 + -0.4 883,145 + -0.5 883,153 + -1.3 883,153 + +0.7
Or see this region's nucleotide sequence