Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_03740 and IAI47_03745 are separated by 170 nucleotides IAI47_03745 and IAI47_03750 are separated by 46 nucleotides IAI47_03750 and IAI47_03755 overlap by 4 nucleotides
IAI47_03740: IAI47_03740 - cell division protein ZapA, at 779,786 to 780,115
_03740
IAI47_03745: IAI47_03745 - YecA family protein, at 780,286 to 780,864
_03745
IAI47_03750: IAI47_03750 - Xaa-Pro aminopeptidase, at 780,911 to 782,233
_03750
IAI47_03755: IAI47_03755 - 2-octaprenyl-6-methoxyphenyl hydroxylase, at 782,230 to 783,408
_03755
Position (kb) 780
781
782
783 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 779.968 kb on + strand, within IAI47_03740 at 779.968 kb on + strand, within IAI47_03740 at 779.968 kb on + strand, within IAI47_03740 at 779.969 kb on - strand, within IAI47_03740 at 779.980 kb on + strand, within IAI47_03740 at 779.980 kb on + strand, within IAI47_03740 at 779.980 kb on + strand, within IAI47_03740 at 779.980 kb on + strand, within IAI47_03740 at 779.980 kb on + strand, within IAI47_03740 at 779.994 kb on - strand, within IAI47_03740 at 780.031 kb on + strand, within IAI47_03740 at 780.031 kb on + strand, within IAI47_03740 at 780.031 kb on + strand, within IAI47_03740 at 780.032 kb on - strand, within IAI47_03740 at 780.093 kb on - strand at 780.098 kb on + strand at 780.134 kb on + strand at 780.134 kb on + strand at 780.154 kb on + strand at 780.154 kb on + strand at 780.154 kb on + strand at 780.155 kb on - strand at 780.184 kb on + strand at 780.184 kb on + strand at 780.184 kb on + strand at 780.184 kb on + strand at 780.184 kb on + strand at 780.184 kb on + strand at 780.184 kb on + strand at 780.185 kb on - strand at 780.185 kb on - strand at 780.185 kb on - strand at 780.185 kb on - strand at 780.185 kb on - strand at 780.185 kb on - strand at 780.185 kb on - strand at 780.185 kb on - strand at 780.185 kb on - strand at 780.185 kb on - strand at 780.185 kb on - strand at 780.185 kb on - strand at 780.185 kb on - strand at 780.185 kb on - strand at 780.185 kb on - strand at 780.186 kb on + strand at 780.186 kb on + strand at 780.186 kb on + strand at 780.186 kb on + strand at 780.186 kb on + strand at 780.187 kb on - strand at 780.187 kb on - strand at 780.187 kb on - strand at 780.187 kb on - strand at 780.188 kb on + strand at 780.188 kb on + strand at 780.188 kb on + strand at 780.188 kb on + strand at 780.188 kb on + strand at 780.189 kb on - strand at 780.189 kb on - strand at 780.189 kb on - strand at 780.189 kb on - strand at 780.189 kb on - strand at 780.189 kb on - strand at 780.189 kb on - strand at 780.189 kb on - strand at 780.201 kb on + strand at 780.201 kb on + strand at 780.202 kb on - strand at 780.202 kb on - strand at 780.202 kb on - strand at 780.206 kb on + strand at 780.206 kb on + strand at 780.207 kb on - strand at 780.207 kb on - strand at 780.207 kb on - strand at 780.238 kb on + strand at 780.239 kb on - strand at 780.239 kb on - strand at 780.244 kb on + strand at 780.244 kb on + strand at 780.245 kb on - strand at 780.249 kb on + strand at 780.249 kb on + strand at 780.249 kb on + strand at 780.250 kb on - strand at 780.260 kb on + strand at 780.260 kb on + strand at 780.261 kb on - strand at 780.285 kb on + strand at 780.285 kb on + strand at 780.285 kb on + strand at 780.285 kb on + strand at 780.286 kb on - strand at 780.286 kb on - strand at 780.293 kb on + strand at 780.293 kb on + strand at 780.293 kb on + strand at 780.294 kb on - strand at 780.294 kb on - strand at 780.294 kb on - strand at 780.313 kb on + strand at 780.313 kb on + strand at 780.354 kb on + strand, within IAI47_03745 at 780.354 kb on + strand, within IAI47_03745 at 780.354 kb on + strand, within IAI47_03745 at 780.354 kb on + strand, within IAI47_03745 at 780.355 kb on - strand, within IAI47_03745 at 780.383 kb on + strand, within IAI47_03745 at 780.434 kb on + strand, within IAI47_03745 at 780.434 kb on + strand, within IAI47_03745 at 780.516 kb on + strand, within IAI47_03745 at 780.516 kb on + strand, within IAI47_03745 at 780.578 kb on + strand, within IAI47_03745 at 780.578 kb on + strand, within IAI47_03745 at 780.578 kb on + strand, within IAI47_03745 at 780.578 kb on + strand, within IAI47_03745 at 780.579 kb on - strand, within IAI47_03745 at 780.639 kb on - strand, within IAI47_03745 at 780.639 kb on - strand, within IAI47_03745 at 780.648 kb on + strand, within IAI47_03745 at 780.648 kb on + strand, within IAI47_03745 at 780.649 kb on - strand, within IAI47_03745 at 780.649 kb on - strand, within IAI47_03745 at 780.657 kb on + strand, within IAI47_03745 at 780.775 kb on + strand, within IAI47_03745 at 780.775 kb on + strand, within IAI47_03745 at 780.775 kb on + strand, within IAI47_03745 at 780.775 kb on + strand, within IAI47_03745 at 780.775 kb on + strand, within IAI47_03745 at 780.776 kb on - strand, within IAI47_03745 at 780.776 kb on - strand, within IAI47_03745 at 780.776 kb on - strand, within IAI47_03745 at 780.776 kb on - strand, within IAI47_03745 at 780.776 kb on - strand, within IAI47_03745 at 780.807 kb on + strand at 780.807 kb on + strand at 780.807 kb on + strand at 780.807 kb on + strand at 780.858 kb on - strand at 780.858 kb on - strand at 780.858 kb on - strand at 780.858 kb on - strand at 780.858 kb on - strand at 780.862 kb on + strand at 780.862 kb on + strand at 780.862 kb on + strand at 780.862 kb on + strand at 780.863 kb on - strand at 780.863 kb on - strand at 780.895 kb on + strand at 780.929 kb on + strand at 780.929 kb on + strand at 781.028 kb on + strand at 781.072 kb on + strand, within IAI47_03750 at 781.230 kb on + strand, within IAI47_03750 at 781.230 kb on + strand, within IAI47_03750 at 781.289 kb on + strand, within IAI47_03750 at 781.307 kb on + strand, within IAI47_03750 at 781.307 kb on + strand, within IAI47_03750 at 781.564 kb on + strand, within IAI47_03750 at 781.564 kb on + strand, within IAI47_03750 at 781.592 kb on + strand, within IAI47_03750 at 781.592 kb on + strand, within IAI47_03750 at 781.592 kb on + strand, within IAI47_03750 at 781.592 kb on + strand, within IAI47_03750 at 781.592 kb on + strand, within IAI47_03750 at 781.592 kb on + strand, within IAI47_03750 at 781.592 kb on + strand, within IAI47_03750 at 781.592 kb on + strand, within IAI47_03750 at 781.592 kb on + strand, within IAI47_03750 at 781.592 kb on + strand, within IAI47_03750 at 781.594 kb on + strand, within IAI47_03750 at 781.594 kb on + strand, within IAI47_03750 at 781.594 kb on + strand, within IAI47_03750 at 781.594 kb on + strand, within IAI47_03750 at 781.720 kb on + strand, within IAI47_03750 at 781.775 kb on + strand, within IAI47_03750 at 781.775 kb on + strand, within IAI47_03750 at 781.775 kb on + strand, within IAI47_03750 at 781.775 kb on + strand, within IAI47_03750 at 781.775 kb on + strand, within IAI47_03750 at 781.867 kb on + strand, within IAI47_03750 at 781.867 kb on + strand, within IAI47_03750 at 781.867 kb on + strand, within IAI47_03750 at 781.949 kb on + strand, within IAI47_03750 at 781.949 kb on + strand, within IAI47_03750 at 781.949 kb on + strand, within IAI47_03750 at 781.949 kb on + strand, within IAI47_03750 at 781.949 kb on + strand, within IAI47_03750 at 781.949 kb on + strand, within IAI47_03750 at 781.949 kb on + strand, within IAI47_03750 at 781.949 kb on + strand, within IAI47_03750 at 781.949 kb on + strand, within IAI47_03750 at 781.949 kb on + strand, within IAI47_03750 at 781.949 kb on + strand, within IAI47_03750 at 781.949 kb on + strand, within IAI47_03750 at 781.949 kb on + strand, within IAI47_03750 at 781.949 kb on + strand, within IAI47_03750 at 781.949 kb on + strand, within IAI47_03750 at 781.949 kb on + strand, within IAI47_03750 at 781.951 kb on + strand, within IAI47_03750 at 781.951 kb on + strand, within IAI47_03750 at 781.951 kb on + strand, within IAI47_03750 at 781.951 kb on + strand, within IAI47_03750 at 781.951 kb on + strand, within IAI47_03750 at 781.951 kb on + strand, within IAI47_03750 at 781.951 kb on + strand, within IAI47_03750 at 781.951 kb on + strand, within IAI47_03750 at 781.951 kb on + strand, within IAI47_03750 at 781.951 kb on + strand, within IAI47_03750 at 781.951 kb on + strand, within IAI47_03750 at 781.951 kb on + strand, within IAI47_03750 at 781.995 kb on + strand, within IAI47_03750 at 781.995 kb on + strand, within IAI47_03750 at 781.995 kb on + strand, within IAI47_03750 at 781.995 kb on + strand, within IAI47_03750 at 781.995 kb on + strand, within IAI47_03750 at 781.995 kb on + strand, within IAI47_03750 at 781.995 kb on + strand, within IAI47_03750 at 782.069 kb on + strand, within IAI47_03750 at 782.069 kb on + strand, within IAI47_03750 at 782.069 kb on + strand, within IAI47_03750 at 782.069 kb on + strand, within IAI47_03750 at 782.069 kb on + strand, within IAI47_03750 at 782.069 kb on + strand, within IAI47_03750 at 782.069 kb on + strand, within IAI47_03750 at 782.071 kb on + strand, within IAI47_03750 at 782.091 kb on + strand, within IAI47_03750
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 779,968 + IAI47_03740 0.55 +0.4 779,968 + IAI47_03740 0.55 +1.3 779,968 + IAI47_03740 0.55 +1.3 779,969 - IAI47_03740 0.55 +2.4 779,980 + IAI47_03740 0.59 +0.6 779,980 + IAI47_03740 0.59 -0.2 779,980 + IAI47_03740 0.59 +0.4 779,980 + IAI47_03740 0.59 -0.3 779,980 + IAI47_03740 0.59 -0.1 779,994 - IAI47_03740 0.63 -0.4 780,031 + IAI47_03740 0.74 +1.0 780,031 + IAI47_03740 0.74 -1.3 780,031 + IAI47_03740 0.74 -1.4 780,032 - IAI47_03740 0.75 -0.9 780,093 - +1.1 780,098 + +0.1 780,134 + -1.4 780,134 + +2.0 780,154 + +0.8 780,154 + -0.9 780,154 + +2.2 780,155 - -0.4 780,184 + -0.6 780,184 + -1.0 780,184 + +1.4 780,184 + -0.3 780,184 + -0.0 780,184 + -1.4 780,184 + -0.6 780,185 - -0.2 780,185 - +1.6 780,185 - +0.7 780,185 - -1.9 780,185 - -1.0 780,185 - -1.7 780,185 - +0.5 780,185 - +0.4 780,185 - +0.4 780,185 - +1.6 780,185 - +1.4 780,185 - -0.2 780,185 - +0.0 780,185 - +1.9 780,185 - +0.7 780,186 + +0.6 780,186 + +0.5 780,186 + -0.7 780,186 + -0.1 780,186 + +0.2 780,187 - +1.7 780,187 - -0.4 780,187 - -0.3 780,187 - -2.3 780,188 + +2.7 780,188 + +3.3 780,188 + -0.4 780,188 + -0.9 780,188 + -0.3 780,189 - -2.4 780,189 - +2.3 780,189 - -0.6 780,189 - +0.4 780,189 - -0.2 780,189 - +0.3 780,189 - +1.3 780,189 - -2.5 780,201 + +0.0 780,201 + -2.2 780,202 - -1.8 780,202 - -0.1 780,202 - -1.9 780,206 + +2.4 780,206 + +2.0 780,207 - -0.9 780,207 - -0.4 780,207 - +0.8 780,238 + -0.8 780,239 - -2.0 780,239 - -0.2 780,244 + -2.7 780,244 + +0.5 780,245 - +0.7 780,249 + -0.5 780,249 + +1.1 780,249 + +0.1 780,250 - -0.1 780,260 + +1.3 780,260 + +0.9 780,261 - -0.4 780,285 + -0.4 780,285 + +0.1 780,285 + -0.0 780,285 + +0.3 780,286 - +0.4 780,286 - +0.7 780,293 + -1.0 780,293 + +0.8 780,293 + +1.5 780,294 - +0.8 780,294 - +0.6 780,294 - -0.8 780,313 + +0.6 780,313 + +0.6 780,354 + IAI47_03745 0.12 +0.4 780,354 + IAI47_03745 0.12 +1.8 780,354 + IAI47_03745 0.12 +0.6 780,354 + IAI47_03745 0.12 +0.4 780,355 - IAI47_03745 0.12 +1.5 780,383 + IAI47_03745 0.17 -0.2 780,434 + IAI47_03745 0.26 -1.7 780,434 + IAI47_03745 0.26 -0.4 780,516 + IAI47_03745 0.40 +2.0 780,516 + IAI47_03745 0.40 +1.9 780,578 + IAI47_03745 0.50 -0.6 780,578 + IAI47_03745 0.50 +0.7 780,578 + IAI47_03745 0.50 +0.8 780,578 + IAI47_03745 0.50 -0.6 780,579 - IAI47_03745 0.51 +0.0 780,639 - IAI47_03745 0.61 +1.6 780,639 - IAI47_03745 0.61 -0.2 780,648 + IAI47_03745 0.63 -0.4 780,648 + IAI47_03745 0.63 +0.6 780,649 - IAI47_03745 0.63 -1.9 780,649 - IAI47_03745 0.63 +4.0 780,657 + IAI47_03745 0.64 +1.2 780,775 + IAI47_03745 0.84 -1.8 780,775 + IAI47_03745 0.84 +0.1 780,775 + IAI47_03745 0.84 -0.7 780,775 + IAI47_03745 0.84 -0.7 780,775 + IAI47_03745 0.84 +0.2 780,776 - IAI47_03745 0.85 -1.3 780,776 - IAI47_03745 0.85 +0.6 780,776 - IAI47_03745 0.85 +1.3 780,776 - IAI47_03745 0.85 +0.8 780,776 - IAI47_03745 0.85 -0.6 780,807 + +0.2 780,807 + +0.8 780,807 + +0.1 780,807 + -0.6 780,858 - -0.0 780,858 - -1.6 780,858 - +0.2 780,858 - +0.2 780,858 - +0.2 780,862 + +1.1 780,862 + +1.7 780,862 + +0.2 780,862 + -0.6 780,863 - -1.3 780,863 - +0.3 780,895 + +0.3 780,929 + -0.5 780,929 + -0.6 781,028 + -1.2 781,072 + IAI47_03750 0.12 -1.0 781,230 + IAI47_03750 0.24 -1.5 781,230 + IAI47_03750 0.24 -1.4 781,289 + IAI47_03750 0.29 -0.0 781,307 + IAI47_03750 0.30 -1.2 781,307 + IAI47_03750 0.30 +0.8 781,564 + IAI47_03750 0.49 -0.2 781,564 + IAI47_03750 0.49 +0.2 781,592 + IAI47_03750 0.51 +0.1 781,592 + IAI47_03750 0.51 -2.2 781,592 + IAI47_03750 0.51 -0.1 781,592 + IAI47_03750 0.51 -0.9 781,592 + IAI47_03750 0.51 -0.7 781,592 + IAI47_03750 0.51 +0.9 781,592 + IAI47_03750 0.51 -1.0 781,592 + IAI47_03750 0.51 +0.9 781,592 + IAI47_03750 0.51 +0.5 781,592 + IAI47_03750 0.51 -0.7 781,594 + IAI47_03750 0.52 +0.1 781,594 + IAI47_03750 0.52 +0.4 781,594 + IAI47_03750 0.52 -0.6 781,594 + IAI47_03750 0.52 -0.6 781,720 + IAI47_03750 0.61 +1.7 781,775 + IAI47_03750 0.65 -0.2 781,775 + IAI47_03750 0.65 -0.0 781,775 + IAI47_03750 0.65 -0.5 781,775 + IAI47_03750 0.65 -1.8 781,775 + IAI47_03750 0.65 +0.7 781,867 + IAI47_03750 0.72 -2.4 781,867 + IAI47_03750 0.72 +1.3 781,867 + IAI47_03750 0.72 -0.0 781,949 + IAI47_03750 0.78 -0.2 781,949 + IAI47_03750 0.78 -0.3 781,949 + IAI47_03750 0.78 -1.1 781,949 + IAI47_03750 0.78 +0.6 781,949 + IAI47_03750 0.78 -0.7 781,949 + IAI47_03750 0.78 +1.4 781,949 + IAI47_03750 0.78 -0.4 781,949 + IAI47_03750 0.78 -0.4 781,949 + IAI47_03750 0.78 +0.8 781,949 + IAI47_03750 0.78 +0.3 781,949 + IAI47_03750 0.78 -0.6 781,949 + IAI47_03750 0.78 -1.0 781,949 + IAI47_03750 0.78 +1.8 781,949 + IAI47_03750 0.78 +0.0 781,949 + IAI47_03750 0.78 -0.4 781,949 + IAI47_03750 0.78 -0.2 781,951 + IAI47_03750 0.79 +0.5 781,951 + IAI47_03750 0.79 +0.8 781,951 + IAI47_03750 0.79 -0.0 781,951 + IAI47_03750 0.79 +1.2 781,951 + IAI47_03750 0.79 +0.7 781,951 + IAI47_03750 0.79 -2.0 781,951 + IAI47_03750 0.79 +1.8 781,951 + IAI47_03750 0.79 -1.5 781,951 + IAI47_03750 0.79 +0.8 781,951 + IAI47_03750 0.79 -0.3 781,951 + IAI47_03750 0.79 -0.3 781,951 + IAI47_03750 0.79 +0.1 781,995 + IAI47_03750 0.82 +0.1 781,995 + IAI47_03750 0.82 -1.1 781,995 + IAI47_03750 0.82 +0.8 781,995 + IAI47_03750 0.82 -2.5 781,995 + IAI47_03750 0.82 +0.8 781,995 + IAI47_03750 0.82 +0.0 781,995 + IAI47_03750 0.82 -2.6 782,069 + IAI47_03750 0.88 -0.4 782,069 + IAI47_03750 0.88 +0.6 782,069 + IAI47_03750 0.88 -0.2 782,069 + IAI47_03750 0.88 -2.9 782,069 + IAI47_03750 0.88 +1.0 782,069 + IAI47_03750 0.88 -0.9 782,069 + IAI47_03750 0.88 +0.3 782,071 + IAI47_03750 0.88 -1.6 782,091 + IAI47_03750 0.89 +1.3
Or see this region's nucleotide sequence