Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_21270 and IAI47_21275 are separated by 9 nucleotides IAI47_21275 and IAI47_21280 are separated by 6 nucleotides IAI47_21280 and IAI47_21285 are separated by 21 nucleotides
IAI47_21270: IAI47_21270 - ABC transporter permease subunit, at 510,477 to 511,163
_21270
IAI47_21275: IAI47_21275 - ABC transporter permease subunit, at 511,173 to 511,898
_21275
IAI47_21280: IAI47_21280 - amino acid ABC transporter substrate-binding protein, at 511,905 to 512,753
_21280
IAI47_21285: IAI47_21285 - amidohydrolase, at 512,775 to 513,962
_21285
Position (kb)
511
512
513 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 510.918 kb on - strand, within IAI47_21270 at 510.918 kb on - strand, within IAI47_21270 at 510.918 kb on - strand, within IAI47_21270 at 510.952 kb on + strand, within IAI47_21270 at 510.952 kb on + strand, within IAI47_21270 at 510.952 kb on + strand, within IAI47_21270 at 510.953 kb on - strand, within IAI47_21270 at 510.959 kb on + strand, within IAI47_21270 at 510.959 kb on + strand, within IAI47_21270 at 510.984 kb on - strand, within IAI47_21270 at 511.004 kb on - strand, within IAI47_21270 at 511.049 kb on + strand, within IAI47_21270 at 511.049 kb on + strand, within IAI47_21270 at 511.100 kb on + strand at 511.100 kb on + strand at 511.100 kb on + strand at 511.100 kb on + strand at 511.101 kb on - strand at 511.146 kb on + strand at 511.146 kb on + strand at 511.146 kb on + strand at 511.147 kb on - strand at 511.174 kb on + strand at 511.175 kb on - strand at 511.250 kb on + strand, within IAI47_21275 at 511.258 kb on + strand, within IAI47_21275 at 511.259 kb on - strand, within IAI47_21275 at 511.259 kb on - strand, within IAI47_21275 at 511.279 kb on + strand, within IAI47_21275 at 511.280 kb on - strand, within IAI47_21275 at 511.280 kb on - strand, within IAI47_21275 at 511.376 kb on + strand, within IAI47_21275 at 511.436 kb on + strand, within IAI47_21275 at 511.436 kb on + strand, within IAI47_21275 at 511.436 kb on + strand, within IAI47_21275 at 511.437 kb on - strand, within IAI47_21275 at 511.437 kb on - strand, within IAI47_21275 at 511.437 kb on - strand, within IAI47_21275 at 511.510 kb on + strand, within IAI47_21275 at 511.511 kb on - strand, within IAI47_21275 at 511.541 kb on - strand, within IAI47_21275 at 511.563 kb on - strand, within IAI47_21275 at 511.657 kb on + strand, within IAI47_21275 at 511.666 kb on + strand, within IAI47_21275 at 511.667 kb on - strand, within IAI47_21275 at 511.708 kb on + strand, within IAI47_21275 at 511.709 kb on - strand, within IAI47_21275 at 511.709 kb on - strand, within IAI47_21275 at 511.765 kb on - strand, within IAI47_21275 at 511.806 kb on + strand, within IAI47_21275 at 511.817 kb on + strand, within IAI47_21275 at 511.817 kb on + strand, within IAI47_21275 at 511.818 kb on - strand, within IAI47_21275 at 511.873 kb on - strand at 511.873 kb on - strand at 511.886 kb on + strand at 511.886 kb on + strand at 511.886 kb on + strand at 511.886 kb on + strand at 511.887 kb on - strand at 511.887 kb on - strand at 511.906 kb on + strand at 511.906 kb on + strand at 511.906 kb on + strand at 511.906 kb on + strand at 512.084 kb on + strand, within IAI47_21280 at 512.197 kb on + strand, within IAI47_21280 at 512.197 kb on + strand, within IAI47_21280 at 512.197 kb on + strand, within IAI47_21280 at 512.197 kb on + strand, within IAI47_21280 at 512.197 kb on + strand, within IAI47_21280 at 512.197 kb on + strand, within IAI47_21280 at 512.197 kb on + strand, within IAI47_21280 at 512.227 kb on + strand, within IAI47_21280 at 512.285 kb on + strand, within IAI47_21280 at 512.419 kb on + strand, within IAI47_21280 at 512.420 kb on - strand, within IAI47_21280 at 512.488 kb on + strand, within IAI47_21280 at 512.488 kb on + strand, within IAI47_21280 at 512.488 kb on + strand, within IAI47_21280 at 512.489 kb on - strand, within IAI47_21280 at 512.489 kb on - strand, within IAI47_21280 at 512.489 kb on - strand, within IAI47_21280 at 512.489 kb on - strand, within IAI47_21280 at 512.492 kb on + strand, within IAI47_21280 at 512.493 kb on - strand, within IAI47_21280 at 512.493 kb on - strand, within IAI47_21280 at 512.545 kb on + strand, within IAI47_21280 at 512.549 kb on + strand, within IAI47_21280 at 512.550 kb on - strand, within IAI47_21280 at 512.550 kb on - strand, within IAI47_21280 at 512.550 kb on - strand, within IAI47_21280 at 512.550 kb on - strand, within IAI47_21280 at 512.593 kb on + strand, within IAI47_21280 at 512.594 kb on - strand, within IAI47_21280 at 512.594 kb on - strand, within IAI47_21280 at 512.594 kb on - strand, within IAI47_21280 at 512.618 kb on + strand, within IAI47_21280 at 512.627 kb on + strand, within IAI47_21280 at 512.638 kb on + strand, within IAI47_21280 at 512.639 kb on - strand, within IAI47_21280 at 512.753 kb on + strand at 512.753 kb on + strand at 512.754 kb on - strand at 512.754 kb on - strand at 512.754 kb on - strand at 512.754 kb on - strand at 512.754 kb on - strand at 512.766 kb on + strand at 512.766 kb on + strand at 512.766 kb on + strand at 512.766 kb on + strand at 512.766 kb on + strand at 512.766 kb on + strand at 512.767 kb on - strand at 512.767 kb on - strand at 512.774 kb on - strand at 512.776 kb on + strand at 512.777 kb on - strand at 512.789 kb on - strand at 512.825 kb on + strand at 512.858 kb on + strand at 512.858 kb on + strand at 512.858 kb on + strand at 512.859 kb on - strand at 512.897 kb on + strand, within IAI47_21285 at 513.043 kb on - strand, within IAI47_21285 at 513.119 kb on + strand, within IAI47_21285 at 513.119 kb on + strand, within IAI47_21285 at 513.120 kb on - strand, within IAI47_21285 at 513.200 kb on + strand, within IAI47_21285 at 513.200 kb on + strand, within IAI47_21285 at 513.201 kb on - strand, within IAI47_21285 at 513.201 kb on - strand, within IAI47_21285 at 513.201 kb on - strand, within IAI47_21285 at 513.296 kb on + strand, within IAI47_21285 at 513.296 kb on + strand, within IAI47_21285 at 513.296 kb on + strand, within IAI47_21285 at 513.296 kb on + strand, within IAI47_21285 at 513.296 kb on + strand, within IAI47_21285 at 513.316 kb on - strand, within IAI47_21285 at 513.357 kb on + strand, within IAI47_21285 at 513.357 kb on + strand, within IAI47_21285 at 513.366 kb on + strand, within IAI47_21285 at 513.367 kb on - strand, within IAI47_21285 at 513.367 kb on - strand, within IAI47_21285 at 513.449 kb on + strand, within IAI47_21285 at 513.449 kb on + strand, within IAI47_21285 at 513.450 kb on - strand, within IAI47_21285 at 513.450 kb on - strand, within IAI47_21285 at 513.563 kb on + strand, within IAI47_21285 at 513.564 kb on - strand, within IAI47_21285 at 513.564 kb on - strand, within IAI47_21285 at 513.585 kb on + strand, within IAI47_21285 at 513.585 kb on + strand, within IAI47_21285 at 513.585 kb on + strand, within IAI47_21285 at 513.586 kb on - strand, within IAI47_21285 at 513.586 kb on - strand, within IAI47_21285 at 513.586 kb on - strand, within IAI47_21285 at 513.588 kb on + strand, within IAI47_21285 at 513.588 kb on + strand, within IAI47_21285 at 513.588 kb on + strand, within IAI47_21285 at 513.588 kb on + strand, within IAI47_21285 at 513.589 kb on - strand, within IAI47_21285 at 513.624 kb on - strand, within IAI47_21285 at 513.624 kb on - strand, within IAI47_21285
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 510,918 - IAI47_21270 0.64 +0.6 510,918 - IAI47_21270 0.64 -1.0 510,918 - IAI47_21270 0.64 +1.8 510,952 + IAI47_21270 0.69 +0.8 510,952 + IAI47_21270 0.69 +0.5 510,952 + IAI47_21270 0.69 +1.2 510,953 - IAI47_21270 0.69 +0.2 510,959 + IAI47_21270 0.70 -0.2 510,959 + IAI47_21270 0.70 -2.8 510,984 - IAI47_21270 0.74 +0.7 511,004 - IAI47_21270 0.77 +1.4 511,049 + IAI47_21270 0.83 -0.2 511,049 + IAI47_21270 0.83 -0.1 511,100 + -1.3 511,100 + -0.7 511,100 + +0.8 511,100 + +0.6 511,101 - +0.6 511,146 + +2.2 511,146 + +0.7 511,146 + -1.5 511,147 - +3.4 511,174 + +0.7 511,175 - -0.4 511,250 + IAI47_21275 0.11 +1.1 511,258 + IAI47_21275 0.12 -1.4 511,259 - IAI47_21275 0.12 -0.5 511,259 - IAI47_21275 0.12 -0.2 511,279 + IAI47_21275 0.15 +0.8 511,280 - IAI47_21275 0.15 +0.9 511,280 - IAI47_21275 0.15 -0.8 511,376 + IAI47_21275 0.28 -1.4 511,436 + IAI47_21275 0.36 +0.3 511,436 + IAI47_21275 0.36 -0.7 511,436 + IAI47_21275 0.36 -0.7 511,437 - IAI47_21275 0.36 +1.1 511,437 - IAI47_21275 0.36 +0.2 511,437 - IAI47_21275 0.36 -0.6 511,510 + IAI47_21275 0.46 -0.5 511,511 - IAI47_21275 0.47 +0.6 511,541 - IAI47_21275 0.51 -0.3 511,563 - IAI47_21275 0.54 +0.3 511,657 + IAI47_21275 0.67 -1.4 511,666 + IAI47_21275 0.68 -1.8 511,667 - IAI47_21275 0.68 +0.9 511,708 + IAI47_21275 0.74 +0.7 511,709 - IAI47_21275 0.74 -1.9 511,709 - IAI47_21275 0.74 +0.6 511,765 - IAI47_21275 0.82 +0.5 511,806 + IAI47_21275 0.87 -0.8 511,817 + IAI47_21275 0.89 +1.0 511,817 + IAI47_21275 0.89 +0.0 511,818 - IAI47_21275 0.89 -0.8 511,873 - -0.1 511,873 - -1.3 511,886 + -0.2 511,886 + -0.5 511,886 + +1.1 511,886 + +0.6 511,887 - -0.8 511,887 - -0.4 511,906 + +0.4 511,906 + -1.4 511,906 + +1.2 511,906 + -0.9 512,084 + IAI47_21280 0.21 -1.0 512,197 + IAI47_21280 0.34 +1.2 512,197 + IAI47_21280 0.34 -1.2 512,197 + IAI47_21280 0.34 -0.5 512,197 + IAI47_21280 0.34 +0.8 512,197 + IAI47_21280 0.34 -0.2 512,197 + IAI47_21280 0.34 -1.3 512,197 + IAI47_21280 0.34 -0.1 512,227 + IAI47_21280 0.38 -0.1 512,285 + IAI47_21280 0.45 +0.6 512,419 + IAI47_21280 0.61 +0.1 512,420 - IAI47_21280 0.61 +0.6 512,488 + IAI47_21280 0.69 +1.8 512,488 + IAI47_21280 0.69 -0.4 512,488 + IAI47_21280 0.69 +1.7 512,489 - IAI47_21280 0.69 -1.5 512,489 - IAI47_21280 0.69 -0.8 512,489 - IAI47_21280 0.69 +0.4 512,489 - IAI47_21280 0.69 +0.6 512,492 + IAI47_21280 0.69 +0.9 512,493 - IAI47_21280 0.69 +0.3 512,493 - IAI47_21280 0.69 -2.1 512,545 + IAI47_21280 0.75 -1.3 512,549 + IAI47_21280 0.76 -0.3 512,550 - IAI47_21280 0.76 +0.9 512,550 - IAI47_21280 0.76 -0.1 512,550 - IAI47_21280 0.76 +0.0 512,550 - IAI47_21280 0.76 +0.7 512,593 + IAI47_21280 0.81 -1.5 512,594 - IAI47_21280 0.81 -0.4 512,594 - IAI47_21280 0.81 -0.8 512,594 - IAI47_21280 0.81 +0.2 512,618 + IAI47_21280 0.84 -1.3 512,627 + IAI47_21280 0.85 -1.4 512,638 + IAI47_21280 0.86 -0.2 512,639 - IAI47_21280 0.86 +0.7 512,753 + -2.2 512,753 + +0.2 512,754 - -0.2 512,754 - -0.1 512,754 - +2.7 512,754 - +0.3 512,754 - +0.1 512,766 + -0.2 512,766 + -1.5 512,766 + -1.0 512,766 + +0.9 512,766 + -1.4 512,766 + -1.3 512,767 - -1.0 512,767 - -0.0 512,774 - +0.5 512,776 + -1.3 512,777 - -0.4 512,789 - -0.1 512,825 + +4.6 512,858 + -1.2 512,858 + -2.7 512,858 + -1.8 512,859 - -0.2 512,897 + IAI47_21285 0.10 -0.5 513,043 - IAI47_21285 0.23 +0.4 513,119 + IAI47_21285 0.29 -0.2 513,119 + IAI47_21285 0.29 +1.1 513,120 - IAI47_21285 0.29 -3.2 513,200 + IAI47_21285 0.36 +2.4 513,200 + IAI47_21285 0.36 +0.3 513,201 - IAI47_21285 0.36 -0.3 513,201 - IAI47_21285 0.36 -0.3 513,201 - IAI47_21285 0.36 +1.2 513,296 + IAI47_21285 0.44 -0.9 513,296 + IAI47_21285 0.44 +0.6 513,296 + IAI47_21285 0.44 -0.8 513,296 + IAI47_21285 0.44 -0.2 513,296 + IAI47_21285 0.44 -0.2 513,316 - IAI47_21285 0.46 -0.4 513,357 + IAI47_21285 0.49 +0.7 513,357 + IAI47_21285 0.49 -1.4 513,366 + IAI47_21285 0.50 -0.8 513,367 - IAI47_21285 0.50 -1.8 513,367 - IAI47_21285 0.50 -0.7 513,449 + IAI47_21285 0.57 -0.9 513,449 + IAI47_21285 0.57 +0.2 513,450 - IAI47_21285 0.57 +0.1 513,450 - IAI47_21285 0.57 -0.8 513,563 + IAI47_21285 0.66 +1.9 513,564 - IAI47_21285 0.66 -2.9 513,564 - IAI47_21285 0.66 -1.4 513,585 + IAI47_21285 0.68 +1.0 513,585 + IAI47_21285 0.68 +0.3 513,585 + IAI47_21285 0.68 -0.7 513,586 - IAI47_21285 0.68 -0.3 513,586 - IAI47_21285 0.68 +0.2 513,586 - IAI47_21285 0.68 -1.3 513,588 + IAI47_21285 0.68 -2.4 513,588 + IAI47_21285 0.68 -1.7 513,588 + IAI47_21285 0.68 -0.3 513,588 + IAI47_21285 0.68 -1.6 513,589 - IAI47_21285 0.69 +0.3 513,624 - IAI47_21285 0.71 +2.5 513,624 - IAI47_21285 0.71 -2.4
Or see this region's nucleotide sequence