Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20280 and IAI47_20285 overlap by 1 nucleotides IAI47_20285 and IAI47_20290 are separated by 43 nucleotides IAI47_20290 and IAI47_20295 are separated by 2 nucleotides IAI47_20295 and IAI47_20300 are separated by 60 nucleotides
IAI47_20280: IAI47_20280 - AAA family ATPase, at 291,187 to 296,727
_20280
IAI47_20285: IAI47_20285 - response regulator, at 296,727 to 297,110
_20285
IAI47_20290: IAI47_20290 - XapX domain-containing protein, at 297,154 to 297,408
_20290
IAI47_20295: IAI47_20295 - DUF1427 family protein, at 297,411 to 297,635
_20295
IAI47_20300: IAI47_20300 - hydrolase, at 297,696 to 298,388
_20300
Position (kb) 297
298 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 296.175 kb on + strand at 296.175 kb on + strand at 296.175 kb on + strand at 296.175 kb on + strand at 296.175 kb on + strand at 296.175 kb on + strand at 296.175 kb on + strand at 296.175 kb on + strand at 296.175 kb on + strand at 296.175 kb on + strand at 296.175 kb on + strand at 296.175 kb on + strand at 296.175 kb on + strand at 296.175 kb on + strand at 296.175 kb on + strand at 296.175 kb on + strand at 296.175 kb on + strand at 296.175 kb on + strand at 296.175 kb on + strand at 296.175 kb on + strand at 296.176 kb on - strand at 296.176 kb on - strand at 296.176 kb on - strand at 296.176 kb on - strand at 296.176 kb on - strand at 296.176 kb on - strand at 296.176 kb on - strand at 296.176 kb on - strand at 296.176 kb on - strand at 296.176 kb on - strand at 296.176 kb on - strand at 296.176 kb on - strand at 296.177 kb on + strand at 296.177 kb on + strand at 296.177 kb on + strand at 296.177 kb on + strand at 296.177 kb on + strand at 296.177 kb on + strand at 296.177 kb on + strand at 296.177 kb on + strand at 296.177 kb on + strand at 296.178 kb on - strand at 296.178 kb on - strand at 296.178 kb on - strand at 296.178 kb on - strand at 296.281 kb on - strand at 296.304 kb on + strand at 296.305 kb on - strand at 296.348 kb on + strand at 296.349 kb on - strand at 296.349 kb on - strand at 296.349 kb on - strand at 296.401 kb on - strand at 296.412 kb on + strand at 296.413 kb on - strand at 296.413 kb on - strand at 296.413 kb on - strand at 296.441 kb on + strand at 296.442 kb on - strand at 296.442 kb on - strand at 296.454 kb on - strand at 296.465 kb on + strand at 296.465 kb on + strand at 296.466 kb on - strand at 296.466 kb on - strand at 296.510 kb on + strand at 296.510 kb on + strand at 296.510 kb on + strand at 296.511 kb on - strand at 296.511 kb on - strand at 296.511 kb on - strand at 296.709 kb on + strand at 296.709 kb on + strand at 296.709 kb on + strand at 296.709 kb on + strand at 296.710 kb on - strand at 296.710 kb on - strand at 296.778 kb on - strand, within IAI47_20285 at 296.778 kb on - strand, within IAI47_20285 at 296.830 kb on + strand, within IAI47_20285 at 296.830 kb on + strand, within IAI47_20285 at 296.830 kb on + strand, within IAI47_20285 at 296.831 kb on - strand, within IAI47_20285 at 296.933 kb on + strand, within IAI47_20285 at 296.948 kb on + strand, within IAI47_20285 at 296.948 kb on + strand, within IAI47_20285 at 296.948 kb on + strand, within IAI47_20285 at 296.948 kb on + strand, within IAI47_20285 at 296.948 kb on + strand, within IAI47_20285 at 296.949 kb on - strand, within IAI47_20285 at 296.949 kb on - strand, within IAI47_20285 at 296.949 kb on - strand, within IAI47_20285 at 296.949 kb on - strand, within IAI47_20285 at 296.949 kb on - strand, within IAI47_20285 at 296.949 kb on - strand, within IAI47_20285 at 296.951 kb on + strand, within IAI47_20285 at 296.951 kb on + strand, within IAI47_20285 at 296.951 kb on + strand, within IAI47_20285 at 296.951 kb on + strand, within IAI47_20285 at 296.951 kb on + strand, within IAI47_20285 at 296.951 kb on + strand, within IAI47_20285 at 296.951 kb on + strand, within IAI47_20285 at 296.951 kb on + strand, within IAI47_20285 at 296.952 kb on - strand, within IAI47_20285 at 296.952 kb on - strand, within IAI47_20285 at 296.952 kb on - strand, within IAI47_20285 at 296.952 kb on - strand, within IAI47_20285 at 296.952 kb on - strand, within IAI47_20285 at 296.952 kb on - strand, within IAI47_20285 at 296.952 kb on - strand, within IAI47_20285 at 296.952 kb on - strand, within IAI47_20285 at 296.952 kb on - strand, within IAI47_20285 at 296.952 kb on - strand, within IAI47_20285 at 296.958 kb on - strand, within IAI47_20285 at 296.991 kb on + strand, within IAI47_20285 at 296.991 kb on + strand, within IAI47_20285 at 296.991 kb on + strand, within IAI47_20285 at 296.991 kb on + strand, within IAI47_20285 at 297.021 kb on + strand, within IAI47_20285 at 297.021 kb on + strand, within IAI47_20285 at 297.022 kb on - strand, within IAI47_20285 at 297.025 kb on + strand, within IAI47_20285 at 297.026 kb on - strand, within IAI47_20285 at 297.092 kb on + strand at 297.092 kb on + strand at 297.092 kb on + strand at 297.093 kb on - strand at 297.093 kb on - strand at 297.093 kb on - strand at 297.093 kb on - strand at 297.093 kb on - strand at 297.116 kb on + strand at 297.116 kb on + strand at 297.116 kb on + strand at 297.117 kb on - strand at 297.155 kb on + strand at 297.155 kb on + strand at 297.155 kb on + strand at 297.156 kb on - strand at 297.156 kb on - strand at 297.156 kb on - strand at 297.165 kb on + strand at 297.165 kb on + strand at 297.165 kb on + strand at 297.166 kb on - strand at 297.166 kb on - strand at 297.258 kb on + strand, within IAI47_20290 at 297.268 kb on + strand, within IAI47_20290 at 297.269 kb on - strand, within IAI47_20290 at 297.271 kb on + strand, within IAI47_20290 at 297.272 kb on - strand, within IAI47_20290 at 297.272 kb on - strand, within IAI47_20290 at 297.272 kb on - strand, within IAI47_20290 at 297.283 kb on + strand, within IAI47_20290 at 297.283 kb on + strand, within IAI47_20290 at 297.284 kb on - strand, within IAI47_20290 at 297.353 kb on + strand, within IAI47_20290 at 297.353 kb on + strand, within IAI47_20290 at 297.353 kb on + strand, within IAI47_20290 at 297.353 kb on + strand, within IAI47_20290 at 297.353 kb on + strand, within IAI47_20290 at 297.353 kb on + strand, within IAI47_20290 at 297.354 kb on - strand, within IAI47_20290 at 297.354 kb on - strand, within IAI47_20290 at 297.354 kb on - strand, within IAI47_20290 at 297.391 kb on - strand at 297.490 kb on - strand, within IAI47_20295 at 297.573 kb on + strand, within IAI47_20295 at 297.573 kb on + strand, within IAI47_20295 at 297.574 kb on - strand, within IAI47_20295 at 297.583 kb on + strand, within IAI47_20295 at 297.583 kb on + strand, within IAI47_20295 at 297.583 kb on + strand, within IAI47_20295 at 297.583 kb on + strand, within IAI47_20295 at 297.584 kb on - strand, within IAI47_20295 at 297.584 kb on - strand, within IAI47_20295 at 297.584 kb on - strand, within IAI47_20295 at 297.584 kb on - strand, within IAI47_20295 at 297.585 kb on + strand, within IAI47_20295 at 297.585 kb on + strand, within IAI47_20295 at 297.585 kb on + strand, within IAI47_20295 at 297.585 kb on + strand, within IAI47_20295 at 297.585 kb on + strand, within IAI47_20295 at 297.585 kb on + strand, within IAI47_20295 at 297.586 kb on - strand, within IAI47_20295 at 297.586 kb on - strand, within IAI47_20295 at 297.586 kb on - strand, within IAI47_20295 at 297.586 kb on - strand, within IAI47_20295 at 297.586 kb on - strand, within IAI47_20295 at 297.586 kb on - strand, within IAI47_20295 at 297.586 kb on - strand, within IAI47_20295 at 297.586 kb on - strand, within IAI47_20295 at 297.586 kb on - strand, within IAI47_20295 at 297.586 kb on - strand, within IAI47_20295 at 297.586 kb on - strand, within IAI47_20295 at 297.596 kb on + strand, within IAI47_20295 at 297.596 kb on + strand, within IAI47_20295 at 297.597 kb on - strand, within IAI47_20295 at 297.625 kb on - strand at 297.625 kb on - strand at 297.698 kb on - strand at 297.703 kb on - strand at 297.761 kb on + strand at 297.761 kb on + strand at 297.761 kb on + strand at 297.761 kb on + strand at 297.762 kb on - strand at 297.762 kb on - strand at 297.762 kb on - strand at 297.762 kb on - strand at 297.762 kb on - strand at 297.762 kb on - strand at 297.762 kb on - strand at 297.762 kb on - strand at 297.775 kb on + strand, within IAI47_20300 at 297.775 kb on + strand, within IAI47_20300 at 297.776 kb on - strand, within IAI47_20300 at 297.781 kb on + strand, within IAI47_20300 at 297.782 kb on - strand, within IAI47_20300 at 297.800 kb on - strand, within IAI47_20300 at 297.841 kb on + strand, within IAI47_20300 at 297.841 kb on + strand, within IAI47_20300 at 297.841 kb on + strand, within IAI47_20300 at 297.841 kb on + strand, within IAI47_20300 at 297.841 kb on + strand, within IAI47_20300 at 297.841 kb on + strand, within IAI47_20300 at 297.841 kb on + strand, within IAI47_20300 at 297.841 kb on + strand, within IAI47_20300 at 297.841 kb on + strand, within IAI47_20300 at 297.842 kb on - strand, within IAI47_20300 at 297.842 kb on - strand, within IAI47_20300 at 297.842 kb on - strand, within IAI47_20300 at 297.842 kb on - strand, within IAI47_20300 at 297.842 kb on - strand, within IAI47_20300 at 297.938 kb on - strand, within IAI47_20300 at 297.953 kb on + strand, within IAI47_20300 at 297.954 kb on - strand, within IAI47_20300 at 297.954 kb on - strand, within IAI47_20300 at 297.954 kb on - strand, within IAI47_20300 at 297.985 kb on + strand, within IAI47_20300 at 297.986 kb on - strand, within IAI47_20300 at 297.986 kb on - strand, within IAI47_20300 at 297.986 kb on - strand, within IAI47_20300 at 297.994 kb on + strand, within IAI47_20300 at 298.145 kb on + strand, within IAI47_20300 at 298.146 kb on - strand, within IAI47_20300 at 298.171 kb on - strand, within IAI47_20300 at 298.181 kb on - strand, within IAI47_20300 at 298.209 kb on + strand, within IAI47_20300 at 298.210 kb on - strand, within IAI47_20300 at 298.232 kb on + strand, within IAI47_20300 at 298.352 kb on + strand at 298.352 kb on + strand at 298.352 kb on + strand at 298.352 kb on + strand at 298.352 kb on + strand at 298.353 kb on - strand at 298.353 kb on - strand at 298.353 kb on - strand at 298.353 kb on - strand at 298.353 kb on - strand at 298.353 kb on - strand at 298.376 kb on + strand at 298.376 kb on + strand at 298.377 kb on - strand at 298.377 kb on - strand at 298.377 kb on - strand at 298.377 kb on - strand at 298.400 kb on + strand at 298.400 kb on + strand at 298.401 kb on - strand at 298.407 kb on + strand at 298.407 kb on + strand at 298.407 kb on + strand at 298.407 kb on + strand at 298.407 kb on + strand at 298.408 kb on - strand at 298.408 kb on - strand at 298.408 kb on - strand at 298.408 kb on - strand at 298.408 kb on - strand at 298.408 kb on - strand at 298.408 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 296,175 + -1.4 296,175 + -0.6 296,175 + -0.0 296,175 + +0.1 296,175 + +1.3 296,175 + +0.7 296,175 + +0.2 296,175 + -0.7 296,175 + -1.3 296,175 + +0.5 296,175 + -0.6 296,175 + +0.4 296,175 + -1.1 296,175 + -0.2 296,175 + +0.5 296,175 + -0.2 296,175 + -1.2 296,175 + +0.4 296,175 + +0.1 296,175 + +0.8 296,176 - -0.9 296,176 - +0.5 296,176 - -0.4 296,176 - +0.5 296,176 - +0.5 296,176 - +0.5 296,176 - +1.9 296,176 - +0.3 296,176 - -0.8 296,176 - -2.0 296,176 - +2.5 296,176 - -1.2 296,177 + -1.9 296,177 + -0.3 296,177 + +3.1 296,177 + +1.9 296,177 + +1.5 296,177 + +0.3 296,177 + -1.2 296,177 + +0.4 296,177 + -0.5 296,178 - +3.7 296,178 - +0.8 296,178 - -0.1 296,178 - -1.1 296,281 - -0.5 296,304 + -0.1 296,305 - +2.3 296,348 + +2.0 296,349 - -0.5 296,349 - -1.1 296,349 - -0.5 296,401 - -2.9 296,412 + +1.6 296,413 - +0.2 296,413 - +1.0 296,413 - -2.4 296,441 + -0.4 296,442 - -1.1 296,442 - -1.1 296,454 - +1.5 296,465 + +1.3 296,465 + -0.4 296,466 - -2.3 296,466 - -0.0 296,510 + +0.5 296,510 + -2.4 296,510 + +0.8 296,511 - -1.6 296,511 - +0.3 296,511 - +0.5 296,709 + -1.7 296,709 + -0.2 296,709 + -0.6 296,709 + -2.1 296,710 - -0.8 296,710 - -0.8 296,778 - IAI47_20285 0.13 +0.7 296,778 - IAI47_20285 0.13 -0.2 296,830 + IAI47_20285 0.27 +0.9 296,830 + IAI47_20285 0.27 +1.1 296,830 + IAI47_20285 0.27 +0.9 296,831 - IAI47_20285 0.27 -0.4 296,933 + IAI47_20285 0.54 -0.4 296,948 + IAI47_20285 0.58 -0.6 296,948 + IAI47_20285 0.58 -1.2 296,948 + IAI47_20285 0.58 -0.2 296,948 + IAI47_20285 0.58 -2.0 296,948 + IAI47_20285 0.58 +2.1 296,949 - IAI47_20285 0.58 +1.5 296,949 - IAI47_20285 0.58 +1.3 296,949 - IAI47_20285 0.58 -0.1 296,949 - IAI47_20285 0.58 -1.5 296,949 - IAI47_20285 0.58 -0.1 296,949 - IAI47_20285 0.58 +2.5 296,951 + IAI47_20285 0.58 -1.1 296,951 + IAI47_20285 0.58 -1.2 296,951 + IAI47_20285 0.58 +0.2 296,951 + IAI47_20285 0.58 -1.5 296,951 + IAI47_20285 0.58 +1.4 296,951 + IAI47_20285 0.58 -0.4 296,951 + IAI47_20285 0.58 -0.6 296,951 + IAI47_20285 0.58 -0.4 296,952 - IAI47_20285 0.59 -0.7 296,952 - IAI47_20285 0.59 +0.2 296,952 - IAI47_20285 0.59 +0.0 296,952 - IAI47_20285 0.59 -0.6 296,952 - IAI47_20285 0.59 -0.6 296,952 - IAI47_20285 0.59 -1.1 296,952 - IAI47_20285 0.59 -1.4 296,952 - IAI47_20285 0.59 -0.9 296,952 - IAI47_20285 0.59 +0.1 296,952 - IAI47_20285 0.59 -1.7 296,958 - IAI47_20285 0.60 -1.2 296,991 + IAI47_20285 0.69 -0.7 296,991 + IAI47_20285 0.69 -0.4 296,991 + IAI47_20285 0.69 -0.2 296,991 + IAI47_20285 0.69 +1.8 297,021 + IAI47_20285 0.77 -1.1 297,021 + IAI47_20285 0.77 -1.1 297,022 - IAI47_20285 0.77 -1.4 297,025 + IAI47_20285 0.78 +0.3 297,026 - IAI47_20285 0.78 +1.5 297,092 + -1.4 297,092 + -1.1 297,092 + -0.5 297,093 - +1.4 297,093 - +2.0 297,093 - -0.9 297,093 - -1.8 297,093 - +1.3 297,116 + -0.5 297,116 + -2.4 297,116 + -1.7 297,117 - -0.6 297,155 + +1.5 297,155 + +1.1 297,155 + +0.8 297,156 - -0.4 297,156 - -0.8 297,156 - +0.3 297,165 + -2.0 297,165 + +0.7 297,165 + +0.1 297,166 - -1.4 297,166 - -0.1 297,258 + IAI47_20290 0.41 +0.5 297,268 + IAI47_20290 0.45 +0.2 297,269 - IAI47_20290 0.45 -1.4 297,271 + IAI47_20290 0.46 -2.1 297,272 - IAI47_20290 0.46 -1.7 297,272 - IAI47_20290 0.46 +0.6 297,272 - IAI47_20290 0.46 +0.9 297,283 + IAI47_20290 0.51 +2.7 297,283 + IAI47_20290 0.51 +2.8 297,284 - IAI47_20290 0.51 +1.2 297,353 + IAI47_20290 0.78 +1.1 297,353 + IAI47_20290 0.78 -1.4 297,353 + IAI47_20290 0.78 +1.3 297,353 + IAI47_20290 0.78 +1.4 297,353 + IAI47_20290 0.78 -0.1 297,353 + IAI47_20290 0.78 -0.5 297,354 - IAI47_20290 0.78 +1.4 297,354 - IAI47_20290 0.78 -0.1 297,354 - IAI47_20290 0.78 +1.6 297,391 - +1.9 297,490 - IAI47_20295 0.35 +0.9 297,573 + IAI47_20295 0.72 +0.2 297,573 + IAI47_20295 0.72 +0.5 297,574 - IAI47_20295 0.72 +0.2 297,583 + IAI47_20295 0.76 -0.5 297,583 + IAI47_20295 0.76 +0.1 297,583 + IAI47_20295 0.76 +0.0 297,583 + IAI47_20295 0.76 -0.5 297,584 - IAI47_20295 0.77 -1.1 297,584 - IAI47_20295 0.77 -1.0 297,584 - IAI47_20295 0.77 -0.2 297,584 - IAI47_20295 0.77 +0.5 297,585 + IAI47_20295 0.77 +1.1 297,585 + IAI47_20295 0.77 -0.7 297,585 + IAI47_20295 0.77 +0.9 297,585 + IAI47_20295 0.77 +0.6 297,585 + IAI47_20295 0.77 +0.3 297,585 + IAI47_20295 0.77 +0.0 297,586 - IAI47_20295 0.78 -1.2 297,586 - IAI47_20295 0.78 +0.3 297,586 - IAI47_20295 0.78 -0.2 297,586 - IAI47_20295 0.78 -1.7 297,586 - IAI47_20295 0.78 +0.5 297,586 - IAI47_20295 0.78 +0.1 297,586 - IAI47_20295 0.78 -0.5 297,586 - IAI47_20295 0.78 +2.9 297,586 - IAI47_20295 0.78 +3.4 297,586 - IAI47_20295 0.78 +1.2 297,586 - IAI47_20295 0.78 +0.1 297,596 + IAI47_20295 0.82 +0.1 297,596 + IAI47_20295 0.82 +0.1 297,597 - IAI47_20295 0.83 +2.5 297,625 - +0.2 297,625 - +0.4 297,698 - +0.1 297,703 - +0.3 297,761 + -0.7 297,761 + -0.4 297,761 + +0.8 297,761 + -0.5 297,762 - -1.4 297,762 - +0.4 297,762 - -0.1 297,762 - +0.3 297,762 - +1.3 297,762 - +1.8 297,762 - -1.0 297,762 - -1.0 297,775 + IAI47_20300 0.11 -0.7 297,775 + IAI47_20300 0.11 -2.0 297,776 - IAI47_20300 0.12 +0.7 297,781 + IAI47_20300 0.12 -1.5 297,782 - IAI47_20300 0.12 -0.0 297,800 - IAI47_20300 0.15 +0.4 297,841 + IAI47_20300 0.21 +0.7 297,841 + IAI47_20300 0.21 +1.0 297,841 + IAI47_20300 0.21 -0.1 297,841 + IAI47_20300 0.21 +0.8 297,841 + IAI47_20300 0.21 -0.2 297,841 + IAI47_20300 0.21 -0.5 297,841 + IAI47_20300 0.21 +1.1 297,841 + IAI47_20300 0.21 -0.3 297,841 + IAI47_20300 0.21 -1.2 297,842 - IAI47_20300 0.21 +0.1 297,842 - IAI47_20300 0.21 -0.8 297,842 - IAI47_20300 0.21 -0.1 297,842 - IAI47_20300 0.21 -1.2 297,842 - IAI47_20300 0.21 +1.4 297,938 - IAI47_20300 0.35 +0.8 297,953 + IAI47_20300 0.37 +0.2 297,954 - IAI47_20300 0.37 -0.4 297,954 - IAI47_20300 0.37 +2.0 297,954 - IAI47_20300 0.37 -0.0 297,985 + IAI47_20300 0.42 -0.6 297,986 - IAI47_20300 0.42 +1.3 297,986 - IAI47_20300 0.42 -0.0 297,986 - IAI47_20300 0.42 -3.1 297,994 + IAI47_20300 0.43 +0.7 298,145 + IAI47_20300 0.65 -1.1 298,146 - IAI47_20300 0.65 +0.5 298,171 - IAI47_20300 0.69 +2.2 298,181 - IAI47_20300 0.70 +1.0 298,209 + IAI47_20300 0.74 +0.5 298,210 - IAI47_20300 0.74 -0.3 298,232 + IAI47_20300 0.77 -2.7 298,352 + +2.0 298,352 + +1.0 298,352 + -1.1 298,352 + -1.0 298,352 + +0.4 298,353 - -0.5 298,353 - -0.1 298,353 - +1.1 298,353 - +0.5 298,353 - +0.2 298,353 - -0.8 298,376 + +1.6 298,376 + +2.1 298,377 - +0.8 298,377 - -1.2 298,377 - +0.4 298,377 - -2.2 298,400 + +1.2 298,400 + -0.2 298,401 - +0.0 298,407 + -0.2 298,407 + +0.7 298,407 + +2.4 298,407 + +0.5 298,407 + -1.1 298,408 - -0.8 298,408 - -0.2 298,408 - -0.7 298,408 - +1.0 298,408 - +0.7 298,408 - +0.3 298,408 - -0.1
Or see this region's nucleotide sequence