Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_19635 and IAI47_19640 are separated by 13 nucleotides IAI47_19640 and IAI47_19645 are separated by 110 nucleotides
IAI47_19635: IAI47_19635 - HAD family hydrolase, at 157,884 to 158,705
_19635
IAI47_19640: IAI47_19640 - helix-turn-helix transcriptional regulator, at 158,719 to 159,123
_19640
IAI47_19645: IAI47_19645 - NAD(P)-dependent alcohol dehydrogenase, at 159,234 to 160,283
_19645
Position (kb) 158
159
160 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 157.788 kb on + strand at 157.789 kb on - strand at 157.789 kb on - strand at 157.789 kb on - strand at 157.819 kb on + strand at 157.826 kb on + strand at 157.827 kb on - strand at 157.827 kb on - strand at 157.827 kb on - strand at 157.827 kb on - strand at 157.827 kb on - strand at 157.868 kb on + strand at 157.868 kb on + strand at 157.868 kb on + strand at 157.868 kb on + strand at 157.869 kb on - strand at 157.869 kb on - strand at 157.869 kb on - strand at 157.893 kb on - strand at 157.893 kb on - strand at 157.893 kb on - strand at 157.934 kb on + strand at 157.934 kb on + strand at 157.935 kb on - strand at 157.937 kb on + strand at 157.937 kb on + strand at 157.937 kb on + strand at 157.937 kb on + strand at 157.937 kb on + strand at 157.937 kb on + strand at 157.937 kb on + strand at 157.937 kb on + strand at 157.937 kb on + strand at 157.938 kb on - strand at 157.938 kb on - strand at 157.938 kb on - strand at 157.938 kb on - strand at 157.938 kb on - strand at 157.938 kb on - strand at 157.938 kb on - strand at 157.944 kb on + strand at 157.944 kb on + strand at 157.944 kb on + strand at 157.944 kb on + strand at 157.944 kb on + strand at 157.945 kb on - strand at 157.971 kb on + strand, within IAI47_19635 at 157.971 kb on + strand, within IAI47_19635 at 157.971 kb on + strand, within IAI47_19635 at 157.972 kb on - strand, within IAI47_19635 at 158.025 kb on + strand, within IAI47_19635 at 158.025 kb on + strand, within IAI47_19635 at 158.026 kb on - strand, within IAI47_19635 at 158.026 kb on - strand, within IAI47_19635 at 158.029 kb on - strand, within IAI47_19635 at 158.057 kb on + strand, within IAI47_19635 at 158.057 kb on + strand, within IAI47_19635 at 158.057 kb on + strand, within IAI47_19635 at 158.057 kb on + strand, within IAI47_19635 at 158.057 kb on + strand, within IAI47_19635 at 158.134 kb on + strand, within IAI47_19635 at 158.134 kb on + strand, within IAI47_19635 at 158.153 kb on + strand, within IAI47_19635 at 158.153 kb on + strand, within IAI47_19635 at 158.154 kb on - strand, within IAI47_19635 at 158.154 kb on - strand, within IAI47_19635 at 158.154 kb on - strand, within IAI47_19635 at 158.154 kb on - strand, within IAI47_19635 at 158.220 kb on + strand, within IAI47_19635 at 158.220 kb on + strand, within IAI47_19635 at 158.220 kb on + strand, within IAI47_19635 at 158.220 kb on + strand, within IAI47_19635 at 158.220 kb on + strand, within IAI47_19635 at 158.221 kb on - strand, within IAI47_19635 at 158.221 kb on - strand, within IAI47_19635 at 158.221 kb on - strand, within IAI47_19635 at 158.221 kb on - strand, within IAI47_19635 at 158.271 kb on + strand, within IAI47_19635 at 158.271 kb on + strand, within IAI47_19635 at 158.358 kb on - strand, within IAI47_19635 at 158.408 kb on + strand, within IAI47_19635 at 158.409 kb on - strand, within IAI47_19635 at 158.409 kb on - strand, within IAI47_19635 at 158.409 kb on - strand, within IAI47_19635 at 158.409 kb on - strand, within IAI47_19635 at 158.409 kb on - strand, within IAI47_19635 at 158.466 kb on - strand, within IAI47_19635 at 158.466 kb on - strand, within IAI47_19635 at 158.485 kb on + strand, within IAI47_19635 at 158.486 kb on - strand, within IAI47_19635 at 158.488 kb on + strand, within IAI47_19635 at 158.488 kb on + strand, within IAI47_19635 at 158.489 kb on - strand, within IAI47_19635 at 158.489 kb on - strand, within IAI47_19635 at 158.576 kb on + strand, within IAI47_19635 at 158.576 kb on + strand, within IAI47_19635 at 158.576 kb on + strand, within IAI47_19635 at 158.576 kb on + strand, within IAI47_19635 at 158.576 kb on + strand, within IAI47_19635 at 158.576 kb on + strand, within IAI47_19635 at 158.576 kb on + strand, within IAI47_19635 at 158.577 kb on - strand, within IAI47_19635 at 158.577 kb on - strand, within IAI47_19635 at 158.577 kb on - strand, within IAI47_19635 at 158.577 kb on - strand, within IAI47_19635 at 158.577 kb on - strand, within IAI47_19635 at 158.577 kb on - strand, within IAI47_19635 at 158.577 kb on - strand, within IAI47_19635 at 158.626 kb on - strand at 158.660 kb on - strand at 158.703 kb on + strand at 158.710 kb on + strand at 158.810 kb on + strand, within IAI47_19640 at 158.810 kb on + strand, within IAI47_19640 at 158.810 kb on + strand, within IAI47_19640 at 158.810 kb on + strand, within IAI47_19640 at 158.810 kb on + strand, within IAI47_19640 at 158.810 kb on + strand, within IAI47_19640 at 158.810 kb on + strand, within IAI47_19640 at 158.810 kb on + strand, within IAI47_19640 at 158.810 kb on + strand, within IAI47_19640 at 158.810 kb on + strand, within IAI47_19640 at 158.811 kb on - strand, within IAI47_19640 at 158.811 kb on - strand, within IAI47_19640 at 158.811 kb on - strand, within IAI47_19640 at 158.811 kb on - strand, within IAI47_19640 at 158.811 kb on - strand, within IAI47_19640 at 158.811 kb on - strand, within IAI47_19640 at 158.811 kb on - strand, within IAI47_19640 at 158.811 kb on - strand, within IAI47_19640 at 158.811 kb on - strand, within IAI47_19640 at 158.811 kb on - strand, within IAI47_19640 at 158.811 kb on - strand, within IAI47_19640 at 158.811 kb on - strand, within IAI47_19640 at 158.811 kb on - strand, within IAI47_19640 at 158.811 kb on - strand, within IAI47_19640 at 158.839 kb on - strand, within IAI47_19640 at 158.839 kb on - strand, within IAI47_19640 at 158.853 kb on + strand, within IAI47_19640 at 158.853 kb on + strand, within IAI47_19640 at 158.853 kb on + strand, within IAI47_19640 at 158.854 kb on - strand, within IAI47_19640 at 158.854 kb on - strand, within IAI47_19640 at 158.854 kb on - strand, within IAI47_19640 at 158.854 kb on - strand, within IAI47_19640 at 158.854 kb on - strand, within IAI47_19640 at 158.854 kb on - strand, within IAI47_19640 at 158.861 kb on + strand, within IAI47_19640 at 159.015 kb on - strand, within IAI47_19640 at 159.089 kb on + strand at 159.095 kb on + strand at 159.157 kb on - strand at 159.180 kb on + strand at 159.181 kb on - strand at 159.181 kb on - strand at 159.186 kb on + strand at 159.186 kb on + strand at 159.186 kb on + strand at 159.186 kb on + strand at 159.186 kb on + strand at 159.186 kb on + strand at 159.186 kb on + strand at 159.186 kb on + strand at 159.186 kb on + strand at 159.186 kb on + strand at 159.186 kb on + strand at 159.186 kb on + strand at 159.187 kb on - strand at 159.187 kb on - strand at 159.187 kb on - strand at 159.187 kb on - strand at 159.187 kb on - strand at 159.187 kb on - strand at 159.187 kb on - strand at 159.188 kb on + strand at 159.189 kb on - strand at 159.205 kb on + strand at 159.205 kb on + strand at 159.242 kb on + strand at 159.243 kb on - strand at 159.278 kb on - strand at 159.298 kb on + strand at 159.299 kb on - strand at 159.299 kb on - strand at 159.299 kb on - strand at 159.340 kb on - strand, within IAI47_19645 at 159.368 kb on + strand, within IAI47_19645 at 159.368 kb on + strand, within IAI47_19645 at 159.369 kb on - strand, within IAI47_19645 at 159.369 kb on - strand, within IAI47_19645 at 159.427 kb on + strand, within IAI47_19645 at 159.428 kb on - strand, within IAI47_19645 at 159.428 kb on - strand, within IAI47_19645 at 159.444 kb on - strand, within IAI47_19645 at 159.479 kb on + strand, within IAI47_19645 at 159.484 kb on + strand, within IAI47_19645 at 159.484 kb on + strand, within IAI47_19645 at 159.484 kb on + strand, within IAI47_19645 at 159.485 kb on - strand, within IAI47_19645 at 159.485 kb on - strand, within IAI47_19645 at 159.497 kb on + strand, within IAI47_19645 at 159.497 kb on + strand, within IAI47_19645 at 159.498 kb on - strand, within IAI47_19645 at 159.498 kb on - strand, within IAI47_19645 at 159.498 kb on - strand, within IAI47_19645 at 159.498 kb on - strand, within IAI47_19645 at 159.549 kb on + strand, within IAI47_19645 at 159.550 kb on - strand, within IAI47_19645 at 159.550 kb on - strand, within IAI47_19645 at 159.550 kb on - strand, within IAI47_19645 at 159.550 kb on - strand, within IAI47_19645 at 159.576 kb on + strand, within IAI47_19645 at 159.577 kb on - strand, within IAI47_19645 at 159.577 kb on - strand, within IAI47_19645 at 159.577 kb on - strand, within IAI47_19645 at 159.577 kb on - strand, within IAI47_19645 at 159.577 kb on - strand, within IAI47_19645 at 159.594 kb on + strand, within IAI47_19645 at 159.594 kb on + strand, within IAI47_19645 at 159.595 kb on - strand, within IAI47_19645 at 159.595 kb on - strand, within IAI47_19645 at 159.605 kb on + strand, within IAI47_19645 at 159.606 kb on - strand, within IAI47_19645 at 159.743 kb on + strand, within IAI47_19645 at 159.744 kb on - strand, within IAI47_19645 at 159.744 kb on - strand, within IAI47_19645 at 159.744 kb on - strand, within IAI47_19645 at 159.750 kb on - strand, within IAI47_19645 at 159.750 kb on - strand, within IAI47_19645 at 159.766 kb on + strand, within IAI47_19645 at 159.766 kb on + strand, within IAI47_19645 at 159.766 kb on + strand, within IAI47_19645 at 159.767 kb on - strand, within IAI47_19645 at 159.767 kb on - strand, within IAI47_19645 at 159.845 kb on + strand, within IAI47_19645 at 159.845 kb on + strand, within IAI47_19645 at 159.890 kb on + strand, within IAI47_19645 at 159.891 kb on - strand, within IAI47_19645 at 159.891 kb on - strand, within IAI47_19645 at 159.950 kb on + strand, within IAI47_19645 at 159.950 kb on + strand, within IAI47_19645 at 159.951 kb on - strand, within IAI47_19645 at 159.951 kb on - strand, within IAI47_19645 at 159.994 kb on - strand, within IAI47_19645 at 159.994 kb on - strand, within IAI47_19645 at 160.055 kb on + strand, within IAI47_19645 at 160.055 kb on + strand, within IAI47_19645 at 160.055 kb on + strand, within IAI47_19645 at 160.055 kb on + strand, within IAI47_19645 at 160.056 kb on - strand, within IAI47_19645 at 160.056 kb on - strand, within IAI47_19645 at 160.056 kb on - strand, within IAI47_19645 at 160.056 kb on - strand, within IAI47_19645 at 160.056 kb on - strand, within IAI47_19645 at 160.097 kb on + strand, within IAI47_19645 at 160.097 kb on + strand, within IAI47_19645 at 160.097 kb on + strand, within IAI47_19645 at 160.098 kb on - strand, within IAI47_19645 at 160.098 kb on - strand, within IAI47_19645 at 160.098 kb on - strand, within IAI47_19645
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 157,788 + -0.1 157,789 - +1.0 157,789 - +0.3 157,789 - +1.7 157,819 + -0.0 157,826 + -0.8 157,827 - -1.4 157,827 - +0.3 157,827 - -0.3 157,827 - +1.6 157,827 - -1.3 157,868 + -0.3 157,868 + +1.3 157,868 + +1.6 157,868 + +1.5 157,869 - -0.0 157,869 - +0.3 157,869 - +0.3 157,893 - -0.5 157,893 - +0.4 157,893 - -0.1 157,934 + +1.9 157,934 + -2.5 157,935 - +0.1 157,937 + +1.1 157,937 + -1.1 157,937 + +1.9 157,937 + +0.5 157,937 + -0.1 157,937 + +1.7 157,937 + -0.9 157,937 + +0.2 157,937 + +1.5 157,938 - -0.2 157,938 - -0.7 157,938 - -1.3 157,938 - +0.4 157,938 - -1.0 157,938 - +1.1 157,938 - -0.8 157,944 + -0.8 157,944 + -1.6 157,944 + +0.4 157,944 + +3.1 157,944 + -1.6 157,945 - -0.7 157,971 + IAI47_19635 0.11 -0.2 157,971 + IAI47_19635 0.11 -0.9 157,971 + IAI47_19635 0.11 -0.9 157,972 - IAI47_19635 0.11 -0.7 158,025 + IAI47_19635 0.17 +0.1 158,025 + IAI47_19635 0.17 -0.0 158,026 - IAI47_19635 0.17 +0.8 158,026 - IAI47_19635 0.17 -0.5 158,029 - IAI47_19635 0.18 -1.2 158,057 + IAI47_19635 0.21 -0.5 158,057 + IAI47_19635 0.21 -1.3 158,057 + IAI47_19635 0.21 -0.4 158,057 + IAI47_19635 0.21 -0.2 158,057 + IAI47_19635 0.21 +0.3 158,134 + IAI47_19635 0.30 +0.4 158,134 + IAI47_19635 0.30 +1.1 158,153 + IAI47_19635 0.33 +0.5 158,153 + IAI47_19635 0.33 -0.7 158,154 - IAI47_19635 0.33 +5.1 158,154 - IAI47_19635 0.33 +0.8 158,154 - IAI47_19635 0.33 +0.5 158,154 - IAI47_19635 0.33 -0.9 158,220 + IAI47_19635 0.41 +0.2 158,220 + IAI47_19635 0.41 +0.4 158,220 + IAI47_19635 0.41 -1.2 158,220 + IAI47_19635 0.41 -0.5 158,220 + IAI47_19635 0.41 -0.2 158,221 - IAI47_19635 0.41 +1.1 158,221 - IAI47_19635 0.41 -2.0 158,221 - IAI47_19635 0.41 +0.5 158,221 - IAI47_19635 0.41 -2.9 158,271 + IAI47_19635 0.47 -0.1 158,271 + IAI47_19635 0.47 +0.1 158,358 - IAI47_19635 0.58 +0.5 158,408 + IAI47_19635 0.64 -1.3 158,409 - IAI47_19635 0.64 +0.3 158,409 - IAI47_19635 0.64 -1.4 158,409 - IAI47_19635 0.64 -1.7 158,409 - IAI47_19635 0.64 +0.2 158,409 - IAI47_19635 0.64 +1.0 158,466 - IAI47_19635 0.71 +0.4 158,466 - IAI47_19635 0.71 -0.0 158,485 + IAI47_19635 0.73 +0.5 158,486 - IAI47_19635 0.73 +0.4 158,488 + IAI47_19635 0.73 +0.0 158,488 + IAI47_19635 0.73 +0.5 158,489 - IAI47_19635 0.74 -1.6 158,489 - IAI47_19635 0.74 +0.3 158,576 + IAI47_19635 0.84 +2.1 158,576 + IAI47_19635 0.84 +0.3 158,576 + IAI47_19635 0.84 -0.8 158,576 + IAI47_19635 0.84 -0.4 158,576 + IAI47_19635 0.84 +2.3 158,576 + IAI47_19635 0.84 +1.6 158,576 + IAI47_19635 0.84 -0.2 158,577 - IAI47_19635 0.84 +0.3 158,577 - IAI47_19635 0.84 -1.8 158,577 - IAI47_19635 0.84 -0.3 158,577 - IAI47_19635 0.84 +0.8 158,577 - IAI47_19635 0.84 -1.0 158,577 - IAI47_19635 0.84 +0.4 158,577 - IAI47_19635 0.84 -0.1 158,626 - +0.1 158,660 - +1.3 158,703 + -0.2 158,710 + +0.7 158,810 + IAI47_19640 0.22 +0.1 158,810 + IAI47_19640 0.22 -0.7 158,810 + IAI47_19640 0.22 +0.7 158,810 + IAI47_19640 0.22 -0.0 158,810 + IAI47_19640 0.22 -0.3 158,810 + IAI47_19640 0.22 -1.8 158,810 + IAI47_19640 0.22 +0.5 158,810 + IAI47_19640 0.22 -0.0 158,810 + IAI47_19640 0.22 -1.2 158,810 + IAI47_19640 0.22 -1.3 158,811 - IAI47_19640 0.23 -0.5 158,811 - IAI47_19640 0.23 -0.7 158,811 - IAI47_19640 0.23 -0.5 158,811 - IAI47_19640 0.23 +0.3 158,811 - IAI47_19640 0.23 -0.3 158,811 - IAI47_19640 0.23 +0.9 158,811 - IAI47_19640 0.23 +1.1 158,811 - IAI47_19640 0.23 +0.7 158,811 - IAI47_19640 0.23 +0.4 158,811 - IAI47_19640 0.23 -1.7 158,811 - IAI47_19640 0.23 +1.2 158,811 - IAI47_19640 0.23 -1.3 158,811 - IAI47_19640 0.23 -0.2 158,811 - IAI47_19640 0.23 -0.2 158,839 - IAI47_19640 0.30 -1.3 158,839 - IAI47_19640 0.30 -0.7 158,853 + IAI47_19640 0.33 -0.7 158,853 + IAI47_19640 0.33 +0.9 158,853 + IAI47_19640 0.33 +1.1 158,854 - IAI47_19640 0.33 +1.1 158,854 - IAI47_19640 0.33 +0.4 158,854 - IAI47_19640 0.33 +1.3 158,854 - IAI47_19640 0.33 -0.6 158,854 - IAI47_19640 0.33 +0.8 158,854 - IAI47_19640 0.33 -0.1 158,861 + IAI47_19640 0.35 -2.5 159,015 - IAI47_19640 0.73 +1.1 159,089 + +1.0 159,095 + -0.1 159,157 - +0.5 159,180 + +0.3 159,181 - -2.6 159,181 - +0.6 159,186 + -1.3 159,186 + -0.1 159,186 + -0.5 159,186 + -0.2 159,186 + +1.2 159,186 + -0.7 159,186 + -1.6 159,186 + -0.3 159,186 + +0.5 159,186 + -0.1 159,186 + -0.2 159,186 + -0.2 159,187 - -2.3 159,187 - +0.3 159,187 - +1.1 159,187 - +2.3 159,187 - +0.9 159,187 - +0.8 159,187 - -0.5 159,188 + -2.5 159,189 - +1.1 159,205 + -1.5 159,205 + +0.5 159,242 + -0.5 159,243 - -0.1 159,278 - -0.6 159,298 + -0.7 159,299 - -0.5 159,299 - -2.1 159,299 - +0.6 159,340 - IAI47_19645 0.10 -0.8 159,368 + IAI47_19645 0.13 +1.8 159,368 + IAI47_19645 0.13 +1.3 159,369 - IAI47_19645 0.13 +0.4 159,369 - IAI47_19645 0.13 +0.5 159,427 + IAI47_19645 0.18 -2.6 159,428 - IAI47_19645 0.18 -1.4 159,428 - IAI47_19645 0.18 +0.7 159,444 - IAI47_19645 0.20 +1.4 159,479 + IAI47_19645 0.23 +0.2 159,484 + IAI47_19645 0.24 +1.4 159,484 + IAI47_19645 0.24 -0.7 159,484 + IAI47_19645 0.24 -0.1 159,485 - IAI47_19645 0.24 -0.5 159,485 - IAI47_19645 0.24 +1.0 159,497 + IAI47_19645 0.25 +0.2 159,497 + IAI47_19645 0.25 +0.9 159,498 - IAI47_19645 0.25 +1.1 159,498 - IAI47_19645 0.25 +0.6 159,498 - IAI47_19645 0.25 +0.7 159,498 - IAI47_19645 0.25 +0.8 159,549 + IAI47_19645 0.30 +0.1 159,550 - IAI47_19645 0.30 -1.2 159,550 - IAI47_19645 0.30 +0.3 159,550 - IAI47_19645 0.30 +1.1 159,550 - IAI47_19645 0.30 -0.5 159,576 + IAI47_19645 0.33 +2.1 159,577 - IAI47_19645 0.33 +1.6 159,577 - IAI47_19645 0.33 -0.7 159,577 - IAI47_19645 0.33 -1.4 159,577 - IAI47_19645 0.33 -0.9 159,577 - IAI47_19645 0.33 -1.2 159,594 + IAI47_19645 0.34 +0.8 159,594 + IAI47_19645 0.34 -0.2 159,595 - IAI47_19645 0.34 +1.6 159,595 - IAI47_19645 0.34 -2.1 159,605 + IAI47_19645 0.35 +1.1 159,606 - IAI47_19645 0.35 -1.2 159,743 + IAI47_19645 0.48 +1.7 159,744 - IAI47_19645 0.49 -1.3 159,744 - IAI47_19645 0.49 -2.1 159,744 - IAI47_19645 0.49 -2.1 159,750 - IAI47_19645 0.49 +2.4 159,750 - IAI47_19645 0.49 +0.9 159,766 + IAI47_19645 0.51 -0.2 159,766 + IAI47_19645 0.51 +0.7 159,766 + IAI47_19645 0.51 -0.0 159,767 - IAI47_19645 0.51 +1.0 159,767 - IAI47_19645 0.51 +2.3 159,845 + IAI47_19645 0.58 -1.5 159,845 + IAI47_19645 0.58 -0.2 159,890 + IAI47_19645 0.62 -0.2 159,891 - IAI47_19645 0.63 -0.1 159,891 - IAI47_19645 0.63 -1.2 159,950 + IAI47_19645 0.68 -1.2 159,950 + IAI47_19645 0.68 -2.0 159,951 - IAI47_19645 0.68 +2.0 159,951 - IAI47_19645 0.68 -1.8 159,994 - IAI47_19645 0.72 +0.2 159,994 - IAI47_19645 0.72 -1.2 160,055 + IAI47_19645 0.78 -0.9 160,055 + IAI47_19645 0.78 +3.2 160,055 + IAI47_19645 0.78 +0.9 160,055 + IAI47_19645 0.78 +0.3 160,056 - IAI47_19645 0.78 +1.3 160,056 - IAI47_19645 0.78 +0.5 160,056 - IAI47_19645 0.78 -0.2 160,056 - IAI47_19645 0.78 -0.5 160,056 - IAI47_19645 0.78 -0.7 160,097 + IAI47_19645 0.82 +0.5 160,097 + IAI47_19645 0.82 +0.6 160,097 + IAI47_19645 0.82 +0.7 160,098 - IAI47_19645 0.82 +0.8 160,098 - IAI47_19645 0.82 -2.5 160,098 - IAI47_19645 0.82 -1.9
Or see this region's nucleotide sequence