Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_17015 and IAI47_17020 overlap by 1 nucleotides IAI47_17020 and IAI47_17025 are separated by 65 nucleotides IAI47_17025 and IAI47_17030 are separated by 17 nucleotides
IAI47_17015: IAI47_17015 - tRNA pseudouridine(55) synthase TruB, at 3,609,837 to 3,610,781
_17015
IAI47_17020: IAI47_17020 - 30S ribosome-binding factor RbfA, at 3,610,781 to 3,611,188
_17020
IAI47_17025: IAI47_17025 - translation initiation factor IF-2, at 3,611,254 to 3,613,947
_17025
IAI47_17030: IAI47_17030 - transcription termination/antitermination protein NusA, at 3,613,965 to 3,615,452
_17030
Position (kb)
3611
3612
3613
3614 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3610.286 kb on + strand, within IAI47_17015 at 3610.302 kb on - strand, within IAI47_17015 at 3610.302 kb on - strand, within IAI47_17015 at 3610.315 kb on + strand, within IAI47_17015 at 3610.315 kb on + strand, within IAI47_17015 at 3610.315 kb on + strand, within IAI47_17015 at 3610.315 kb on + strand, within IAI47_17015 at 3610.316 kb on - strand, within IAI47_17015 at 3610.316 kb on - strand, within IAI47_17015 at 3610.366 kb on + strand, within IAI47_17015 at 3610.366 kb on + strand, within IAI47_17015 at 3610.366 kb on + strand, within IAI47_17015 at 3610.366 kb on + strand, within IAI47_17015 at 3610.366 kb on + strand, within IAI47_17015 at 3610.367 kb on + strand, within IAI47_17015 at 3610.367 kb on - strand, within IAI47_17015 at 3610.372 kb on + strand, within IAI47_17015 at 3610.372 kb on + strand, within IAI47_17015 at 3610.372 kb on + strand, within IAI47_17015 at 3610.373 kb on - strand, within IAI47_17015 at 3610.373 kb on - strand, within IAI47_17015 at 3610.373 kb on - strand, within IAI47_17015 at 3610.373 kb on - strand, within IAI47_17015 at 3610.555 kb on + strand, within IAI47_17015 at 3610.556 kb on - strand, within IAI47_17015 at 3610.556 kb on - strand, within IAI47_17015 at 3610.579 kb on + strand, within IAI47_17015 at 3610.579 kb on + strand, within IAI47_17015 at 3610.580 kb on - strand, within IAI47_17015 at 3610.580 kb on - strand, within IAI47_17015 at 3610.667 kb on + strand, within IAI47_17015 at 3610.679 kb on + strand, within IAI47_17015 at 3610.680 kb on - strand, within IAI47_17015 at 3610.686 kb on + strand, within IAI47_17015 at 3610.686 kb on + strand, within IAI47_17015 at 3610.686 kb on + strand, within IAI47_17015 at 3610.686 kb on + strand, within IAI47_17015 at 3610.687 kb on - strand at 3610.687 kb on - strand at 3610.687 kb on - strand at 3610.687 kb on - strand at 3610.687 kb on - strand at 3610.727 kb on + strand at 3610.727 kb on + strand at 3610.727 kb on + strand at 3610.727 kb on + strand at 3610.728 kb on - strand at 3610.728 kb on - strand at 3610.728 kb on - strand at 3610.782 kb on + strand at 3610.783 kb on - strand at 3610.783 kb on - strand at 3610.783 kb on - strand at 3610.865 kb on - strand, within IAI47_17020 at 3610.865 kb on - strand, within IAI47_17020 at 3610.865 kb on - strand, within IAI47_17020 at 3610.865 kb on - strand, within IAI47_17020 at 3610.865 kb on - strand, within IAI47_17020 at 3610.865 kb on - strand, within IAI47_17020 at 3610.881 kb on - strand, within IAI47_17020 at 3610.881 kb on - strand, within IAI47_17020 at 3611.206 kb on - strand at 3611.253 kb on - strand at 3611.253 kb on - strand at 3611.253 kb on - strand at 3611.256 kb on - strand at 3613.964 kb on - strand at 3613.964 kb on - strand at 3613.964 kb on - strand at 3613.964 kb on - strand at 3613.964 kb on - strand at 3613.967 kb on - strand at 3613.995 kb on - strand at 3613.995 kb on - strand at 3613.995 kb on - strand at 3613.995 kb on - strand at 3613.995 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 3,610,286 + IAI47_17015 0.48 +0.7 3,610,302 - IAI47_17015 0.49 +0.1 3,610,302 - IAI47_17015 0.49 -0.2 3,610,315 + IAI47_17015 0.51 +0.0 3,610,315 + IAI47_17015 0.51 +0.4 3,610,315 + IAI47_17015 0.51 -0.9 3,610,315 + IAI47_17015 0.51 +0.4 3,610,316 - IAI47_17015 0.51 -1.9 3,610,316 - IAI47_17015 0.51 -0.3 3,610,366 + IAI47_17015 0.56 -0.8 3,610,366 + IAI47_17015 0.56 -1.0 3,610,366 + IAI47_17015 0.56 -0.8 3,610,366 + IAI47_17015 0.56 -1.4 3,610,366 + IAI47_17015 0.56 -0.8 3,610,367 + IAI47_17015 0.56 +2.1 3,610,367 - IAI47_17015 0.56 -0.6 3,610,372 + IAI47_17015 0.57 +0.6 3,610,372 + IAI47_17015 0.57 +0.3 3,610,372 + IAI47_17015 0.57 +1.3 3,610,373 - IAI47_17015 0.57 +2.5 3,610,373 - IAI47_17015 0.57 -0.6 3,610,373 - IAI47_17015 0.57 -2.2 3,610,373 - IAI47_17015 0.57 +0.7 3,610,555 + IAI47_17015 0.76 -0.3 3,610,556 - IAI47_17015 0.76 +1.7 3,610,556 - IAI47_17015 0.76 +0.3 3,610,579 + IAI47_17015 0.79 +0.7 3,610,579 + IAI47_17015 0.79 +0.3 3,610,580 - IAI47_17015 0.79 -0.5 3,610,580 - IAI47_17015 0.79 -0.0 3,610,667 + IAI47_17015 0.88 +1.2 3,610,679 + IAI47_17015 0.89 -0.7 3,610,680 - IAI47_17015 0.89 -0.3 3,610,686 + IAI47_17015 0.90 -0.5 3,610,686 + IAI47_17015 0.90 -1.4 3,610,686 + IAI47_17015 0.90 -0.9 3,610,686 + IAI47_17015 0.90 -0.0 3,610,687 - +0.7 3,610,687 - +1.4 3,610,687 - -0.8 3,610,687 - +1.7 3,610,687 - +1.2 3,610,727 + +0.2 3,610,727 + -0.9 3,610,727 + +2.0 3,610,727 + +0.5 3,610,728 - +0.9 3,610,728 - -1.7 3,610,728 - -1.2 3,610,782 + -0.4 3,610,783 - -0.4 3,610,783 - -0.2 3,610,783 - -0.3 3,610,865 - IAI47_17020 0.21 -2.4 3,610,865 - IAI47_17020 0.21 +1.5 3,610,865 - IAI47_17020 0.21 -0.6 3,610,865 - IAI47_17020 0.21 -0.5 3,610,865 - IAI47_17020 0.21 -1.2 3,610,865 - IAI47_17020 0.21 +0.4 3,610,881 - IAI47_17020 0.25 +0.2 3,610,881 - IAI47_17020 0.25 -0.6 3,611,206 - +0.7 3,611,253 - -3.2 3,611,253 - -1.3 3,611,253 - +0.7 3,611,256 - -0.9 3,613,964 - +0.9 3,613,964 - -0.0 3,613,964 - -2.0 3,613,964 - -1.1 3,613,964 - -1.0 3,613,967 - +1.0 3,613,995 - -2.4 3,613,995 - -2.9 3,613,995 - -1.2 3,613,995 - -1.3 3,613,995 - -0.4
Or see this region's nucleotide sequence