Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_04715 and IAI47_04720 are separated by 780 nucleotides IAI47_04720 and IAI47_04725 overlap by 14 nucleotides IAI47_04725 and IAI47_04730 are separated by 200 nucleotides
IAI47_04715: IAI47_04715 - integrase, at 993,638 to 994,327
_04715
IAI47_04720: IAI47_04720 - endonuclease/exonuclease/phosphatase family protein, at 995,108 to 996,028
_04720
IAI47_04725: IAI47_04725 - hypothetical protein, at 996,015 to 996,479
_04725
IAI47_04730: IAI47_04730 - toprim domain-containing protein, at 996,680 to 998,995
_04730
Position (kb) 995
996
997 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 994.119 kb on + strand, within IAI47_04715 at 994.119 kb on + strand, within IAI47_04715 at 994.119 kb on + strand, within IAI47_04715 at 994.119 kb on + strand, within IAI47_04715 at 994.120 kb on - strand, within IAI47_04715 at 994.121 kb on + strand, within IAI47_04715 at 994.121 kb on + strand, within IAI47_04715 at 994.121 kb on + strand, within IAI47_04715 at 994.121 kb on + strand, within IAI47_04715 at 994.122 kb on - strand, within IAI47_04715 at 994.122 kb on - strand, within IAI47_04715 at 994.122 kb on - strand, within IAI47_04715 at 994.122 kb on - strand, within IAI47_04715 at 994.126 kb on + strand, within IAI47_04715 at 994.126 kb on + strand, within IAI47_04715 at 994.126 kb on + strand, within IAI47_04715 at 994.126 kb on + strand, within IAI47_04715 at 994.126 kb on + strand, within IAI47_04715 at 994.126 kb on + strand, within IAI47_04715 at 994.127 kb on - strand, within IAI47_04715 at 994.127 kb on - strand, within IAI47_04715 at 994.132 kb on + strand, within IAI47_04715 at 994.132 kb on + strand, within IAI47_04715 at 994.132 kb on + strand, within IAI47_04715 at 994.132 kb on + strand, within IAI47_04715 at 994.132 kb on + strand, within IAI47_04715 at 994.133 kb on - strand, within IAI47_04715 at 994.133 kb on - strand, within IAI47_04715 at 994.133 kb on - strand, within IAI47_04715 at 994.133 kb on - strand, within IAI47_04715 at 994.133 kb on - strand, within IAI47_04715 at 994.137 kb on - strand, within IAI47_04715 at 994.137 kb on - strand, within IAI47_04715 at 994.163 kb on + strand, within IAI47_04715 at 994.164 kb on - strand, within IAI47_04715 at 994.164 kb on - strand, within IAI47_04715 at 994.164 kb on - strand, within IAI47_04715 at 994.189 kb on + strand, within IAI47_04715 at 994.279 kb on + strand at 994.279 kb on + strand at 994.280 kb on - strand at 994.280 kb on - strand at 994.297 kb on + strand at 994.298 kb on - strand at 994.300 kb on + strand at 994.310 kb on - strand at 994.310 kb on - strand at 994.395 kb on + strand at 994.395 kb on + strand at 994.395 kb on + strand at 994.395 kb on + strand at 994.395 kb on + strand at 994.396 kb on - strand at 994.396 kb on - strand at 994.396 kb on - strand at 994.416 kb on + strand at 994.416 kb on + strand at 994.416 kb on + strand at 994.417 kb on - strand at 994.417 kb on - strand at 994.560 kb on - strand at 994.587 kb on + strand at 994.587 kb on + strand at 994.587 kb on + strand at 994.587 kb on + strand at 994.588 kb on - strand at 994.588 kb on - strand at 994.588 kb on - strand at 994.603 kb on - strand at 994.639 kb on - strand at 994.656 kb on - strand at 994.667 kb on - strand at 994.678 kb on - strand at 994.682 kb on + strand at 994.702 kb on + strand at 994.702 kb on + strand at 994.703 kb on - strand at 994.765 kb on + strand at 994.765 kb on + strand at 994.765 kb on + strand at 994.765 kb on + strand at 994.765 kb on + strand at 994.765 kb on + strand at 994.765 kb on + strand at 994.766 kb on - strand at 994.766 kb on - strand at 994.766 kb on - strand at 994.768 kb on + strand at 994.768 kb on + strand at 994.768 kb on + strand at 994.768 kb on + strand at 994.768 kb on + strand at 994.768 kb on + strand at 994.768 kb on + strand at 994.769 kb on - strand at 994.769 kb on - strand at 994.769 kb on - strand at 994.769 kb on - strand at 994.769 kb on - strand at 994.825 kb on + strand at 994.825 kb on + strand at 994.831 kb on + strand at 994.831 kb on + strand at 994.831 kb on + strand at 994.832 kb on - strand at 994.842 kb on + strand at 994.842 kb on + strand at 994.842 kb on + strand at 994.842 kb on + strand at 994.842 kb on + strand at 994.843 kb on - strand at 994.845 kb on + strand at 994.845 kb on + strand at 994.845 kb on + strand at 994.846 kb on - strand at 994.846 kb on - strand at 994.846 kb on - strand at 994.846 kb on - strand at 994.855 kb on + strand at 994.855 kb on + strand at 994.855 kb on + strand at 994.855 kb on + strand at 994.855 kb on + strand at 994.855 kb on + strand at 994.855 kb on + strand at 994.856 kb on - strand at 994.856 kb on - strand at 994.856 kb on - strand at 994.856 kb on - strand at 994.856 kb on - strand at 994.861 kb on + strand at 994.862 kb on - strand at 994.896 kb on - strand at 994.896 kb on - strand at 994.936 kb on + strand at 994.936 kb on + strand at 994.936 kb on + strand at 994.936 kb on + strand at 994.936 kb on + strand at 994.936 kb on + strand at 994.937 kb on - strand at 994.937 kb on - strand at 994.937 kb on - strand at 994.937 kb on - strand at 994.937 kb on - strand at 994.949 kb on + strand at 994.949 kb on + strand at 994.950 kb on - strand at 994.975 kb on + strand at 995.024 kb on + strand at 995.025 kb on - strand at 995.033 kb on + strand at 995.033 kb on + strand at 995.034 kb on - strand at 995.034 kb on - strand at 995.062 kb on - strand at 995.062 kb on - strand at 995.082 kb on - strand at 995.082 kb on - strand at 995.085 kb on + strand at 995.085 kb on + strand at 995.086 kb on - strand at 995.127 kb on + strand at 995.127 kb on + strand at 995.131 kb on + strand at 995.131 kb on + strand at 995.132 kb on - strand at 995.132 kb on - strand at 995.132 kb on - strand at 995.133 kb on + strand at 995.133 kb on + strand at 995.133 kb on + strand at 995.133 kb on + strand at 995.133 kb on + strand at 995.133 kb on + strand at 995.134 kb on - strand at 995.134 kb on - strand at 995.134 kb on - strand at 995.147 kb on - strand at 995.194 kb on + strand at 995.194 kb on + strand at 995.195 kb on - strand at 995.201 kb on + strand, within IAI47_04720 at 995.202 kb on - strand, within IAI47_04720 at 995.214 kb on + strand, within IAI47_04720 at 995.217 kb on + strand, within IAI47_04720 at 995.217 kb on + strand, within IAI47_04720 at 995.251 kb on + strand, within IAI47_04720 at 995.251 kb on + strand, within IAI47_04720 at 995.251 kb on + strand, within IAI47_04720 at 995.253 kb on + strand, within IAI47_04720 at 995.253 kb on + strand, within IAI47_04720 at 995.254 kb on - strand, within IAI47_04720 at 995.259 kb on + strand, within IAI47_04720 at 995.260 kb on - strand, within IAI47_04720 at 995.260 kb on - strand, within IAI47_04720 at 995.290 kb on + strand, within IAI47_04720 at 995.317 kb on + strand, within IAI47_04720 at 995.322 kb on + strand, within IAI47_04720 at 995.325 kb on - strand, within IAI47_04720 at 995.334 kb on + strand, within IAI47_04720 at 995.335 kb on - strand, within IAI47_04720 at 995.335 kb on - strand, within IAI47_04720 at 995.362 kb on + strand, within IAI47_04720 at 995.364 kb on + strand, within IAI47_04720 at 995.364 kb on + strand, within IAI47_04720 at 995.364 kb on + strand, within IAI47_04720 at 995.365 kb on - strand, within IAI47_04720 at 995.370 kb on + strand, within IAI47_04720 at 995.437 kb on + strand, within IAI47_04720 at 995.447 kb on + strand, within IAI47_04720 at 995.574 kb on + strand, within IAI47_04720 at 995.574 kb on + strand, within IAI47_04720 at 995.574 kb on + strand, within IAI47_04720 at 995.621 kb on - strand, within IAI47_04720 at 995.700 kb on + strand, within IAI47_04720 at 995.700 kb on + strand, within IAI47_04720 at 995.700 kb on + strand, within IAI47_04720 at 995.700 kb on + strand, within IAI47_04720 at 995.706 kb on + strand, within IAI47_04720 at 995.707 kb on - strand, within IAI47_04720 at 995.707 kb on - strand, within IAI47_04720 at 995.727 kb on + strand, within IAI47_04720 at 995.733 kb on - strand, within IAI47_04720 at 995.777 kb on - strand, within IAI47_04720 at 995.810 kb on + strand, within IAI47_04720 at 995.811 kb on - strand, within IAI47_04720 at 995.811 kb on - strand, within IAI47_04720 at 995.814 kb on - strand, within IAI47_04720 at 995.833 kb on + strand, within IAI47_04720 at 995.880 kb on + strand, within IAI47_04720 at 995.948 kb on + strand at 995.953 kb on - strand at 996.099 kb on - strand, within IAI47_04725 at 996.100 kb on + strand, within IAI47_04725 at 996.166 kb on + strand, within IAI47_04725 at 996.166 kb on + strand, within IAI47_04725 at 996.171 kb on - strand, within IAI47_04725 at 996.214 kb on + strand, within IAI47_04725 at 996.303 kb on + strand, within IAI47_04725 at 996.304 kb on - strand, within IAI47_04725 at 996.305 kb on + strand, within IAI47_04725 at 996.306 kb on - strand, within IAI47_04725 at 996.306 kb on - strand, within IAI47_04725 at 996.340 kb on + strand, within IAI47_04725 at 996.341 kb on - strand, within IAI47_04725 at 996.413 kb on + strand, within IAI47_04725 at 996.463 kb on + strand at 996.467 kb on + strand at 996.505 kb on + strand at 996.506 kb on - strand at 996.510 kb on + strand at 996.511 kb on - strand at 996.511 kb on - strand at 996.512 kb on + strand at 996.512 kb on + strand at 996.512 kb on + strand at 996.513 kb on - strand at 996.513 kb on - strand at 996.538 kb on - strand at 996.558 kb on + strand at 996.559 kb on - strand at 996.559 kb on - strand at 996.559 kb on - strand at 996.561 kb on + strand at 996.561 kb on + strand at 996.561 kb on + strand at 996.561 kb on + strand at 996.562 kb on - strand at 996.562 kb on - strand at 996.583 kb on + strand at 996.583 kb on + strand at 996.583 kb on + strand at 996.583 kb on + strand at 996.584 kb on - strand at 996.584 kb on - strand at 996.584 kb on - strand at 996.584 kb on - strand at 996.590 kb on + strand at 996.590 kb on + strand at 996.590 kb on + strand at 996.590 kb on + strand at 996.590 kb on + strand at 996.591 kb on - strand at 996.591 kb on - strand at 996.591 kb on - strand at 996.593 kb on + strand at 996.593 kb on + strand at 996.593 kb on + strand at 996.593 kb on + strand at 996.594 kb on - strand at 996.594 kb on - strand at 996.594 kb on - strand at 996.605 kb on + strand at 996.628 kb on + strand at 996.629 kb on - strand at 996.677 kb on - strand at 996.677 kb on - strand at 996.677 kb on - strand at 996.681 kb on + strand at 996.681 kb on + strand at 996.681 kb on + strand at 996.682 kb on - strand at 996.682 kb on - strand at 996.682 kb on - strand at 996.682 kb on - strand at 996.682 kb on - strand at 996.730 kb on + strand at 996.730 kb on + strand at 996.730 kb on + strand at 996.731 kb on - strand at 996.751 kb on + strand at 996.752 kb on - strand at 996.752 kb on - strand at 996.766 kb on + strand at 996.767 kb on - strand at 996.767 kb on - strand at 996.774 kb on - strand at 996.783 kb on + strand at 996.783 kb on + strand at 996.783 kb on + strand at 996.784 kb on - strand at 996.784 kb on - strand at 996.784 kb on - strand at 996.859 kb on + strand at 996.860 kb on - strand at 996.860 kb on - strand at 996.866 kb on + strand at 996.866 kb on + strand at 996.866 kb on + strand at 996.866 kb on + strand at 996.867 kb on - strand at 996.870 kb on + strand at 996.870 kb on + strand at 996.870 kb on + strand at 996.870 kb on + strand at 996.870 kb on + strand at 996.870 kb on + strand at 996.870 kb on + strand at 996.870 kb on + strand at 996.870 kb on + strand at 996.870 kb on + strand at 996.870 kb on + strand at 996.870 kb on + strand at 996.870 kb on + strand at 996.871 kb on - strand at 996.871 kb on - strand at 996.871 kb on - strand at 996.871 kb on - strand at 996.871 kb on - strand at 996.871 kb on - strand at 996.871 kb on - strand at 996.871 kb on - strand at 996.871 kb on - strand at 996.871 kb on - strand at 996.871 kb on - strand at 996.871 kb on - strand at 996.871 kb on - strand at 996.871 kb on - strand at 996.871 kb on - strand at 996.879 kb on + strand at 996.879 kb on + strand at 996.880 kb on - strand at 996.886 kb on - strand at 996.886 kb on - strand at 996.886 kb on - strand at 996.925 kb on + strand, within IAI47_04730 at 996.925 kb on + strand, within IAI47_04730 at 996.960 kb on + strand, within IAI47_04730 at 996.997 kb on + strand, within IAI47_04730
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 994,119 + IAI47_04715 0.70 +0.1 994,119 + IAI47_04715 0.70 +0.1 994,119 + IAI47_04715 0.70 -0.7 994,119 + IAI47_04715 0.70 -1.6 994,120 - IAI47_04715 0.70 +0.1 994,121 + IAI47_04715 0.70 -0.6 994,121 + IAI47_04715 0.70 +1.2 994,121 + IAI47_04715 0.70 -0.1 994,121 + IAI47_04715 0.70 -0.7 994,122 - IAI47_04715 0.70 -2.4 994,122 - IAI47_04715 0.70 -0.1 994,122 - IAI47_04715 0.70 +0.7 994,122 - IAI47_04715 0.70 +0.6 994,126 + IAI47_04715 0.71 -0.4 994,126 + IAI47_04715 0.71 +0.6 994,126 + IAI47_04715 0.71 -0.4 994,126 + IAI47_04715 0.71 +1.4 994,126 + IAI47_04715 0.71 +0.1 994,126 + IAI47_04715 0.71 -0.2 994,127 - IAI47_04715 0.71 -0.5 994,127 - IAI47_04715 0.71 -0.8 994,132 + IAI47_04715 0.72 -2.4 994,132 + IAI47_04715 0.72 -0.7 994,132 + IAI47_04715 0.72 +1.7 994,132 + IAI47_04715 0.72 +0.2 994,132 + IAI47_04715 0.72 -1.2 994,133 - IAI47_04715 0.72 +0.1 994,133 - IAI47_04715 0.72 +0.1 994,133 - IAI47_04715 0.72 +0.7 994,133 - IAI47_04715 0.72 +0.1 994,133 - IAI47_04715 0.72 +0.8 994,137 - IAI47_04715 0.72 -1.0 994,137 - IAI47_04715 0.72 -1.5 994,163 + IAI47_04715 0.76 -1.3 994,164 - IAI47_04715 0.76 +0.0 994,164 - IAI47_04715 0.76 +0.2 994,164 - IAI47_04715 0.76 +2.7 994,189 + IAI47_04715 0.80 +1.7 994,279 + -0.4 994,279 + -1.7 994,280 - -0.6 994,280 - +0.7 994,297 + -0.1 994,298 - +3.4 994,300 + +0.0 994,310 - +0.6 994,310 - -0.9 994,395 + +1.4 994,395 + +0.4 994,395 + +1.8 994,395 + +0.6 994,395 + +0.6 994,396 - +1.7 994,396 - -1.5 994,396 - -2.2 994,416 + -1.1 994,416 + +1.3 994,416 + -1.7 994,417 - +0.3 994,417 - -0.9 994,560 - +1.0 994,587 + +1.2 994,587 + +0.2 994,587 + +0.3 994,587 + +1.4 994,588 - +2.4 994,588 - +0.6 994,588 - -0.7 994,603 - +0.4 994,639 - +0.1 994,656 - +0.2 994,667 - -2.3 994,678 - +2.4 994,682 + +0.7 994,702 + -0.4 994,702 + -0.0 994,703 - -0.0 994,765 + -1.0 994,765 + -1.0 994,765 + +0.4 994,765 + -0.6 994,765 + -1.7 994,765 + +1.3 994,765 + +1.0 994,766 - -1.4 994,766 - +1.9 994,766 - +0.5 994,768 + -0.6 994,768 + +1.4 994,768 + -0.5 994,768 + +1.8 994,768 + -0.2 994,768 + +0.8 994,768 + +0.5 994,769 - +0.2 994,769 - -1.3 994,769 - +0.8 994,769 - -1.7 994,769 - +0.2 994,825 + +1.4 994,825 + -0.2 994,831 + -1.2 994,831 + -0.7 994,831 + -2.6 994,832 - -0.0 994,842 + -0.3 994,842 + -1.5 994,842 + +0.6 994,842 + -0.1 994,842 + -0.5 994,843 - +0.1 994,845 + -0.3 994,845 + -0.2 994,845 + -0.2 994,846 - -0.2 994,846 - +0.6 994,846 - -0.9 994,846 - -0.4 994,855 + +0.7 994,855 + +0.2 994,855 + +0.7 994,855 + +0.2 994,855 + -0.1 994,855 + +1.1 994,855 + -2.0 994,856 - +1.5 994,856 - -1.5 994,856 - -1.5 994,856 - -0.7 994,856 - +1.4 994,861 + +0.2 994,862 - -0.0 994,896 - +0.1 994,896 - -1.2 994,936 + +0.6 994,936 + +2.1 994,936 + +0.8 994,936 + +0.2 994,936 + -0.4 994,936 + +1.2 994,937 - +0.3 994,937 - -1.6 994,937 - -0.2 994,937 - +2.1 994,937 - -0.4 994,949 + -0.6 994,949 + +1.1 994,950 - -1.8 994,975 + -0.8 995,024 + +0.3 995,025 - -0.4 995,033 + +0.2 995,033 + -0.7 995,034 - -0.7 995,034 - +0.7 995,062 - +2.1 995,062 - +1.8 995,082 - +0.1 995,082 - -1.0 995,085 + -0.4 995,085 + +0.7 995,086 - -0.4 995,127 + +0.1 995,127 + -1.3 995,131 + +0.8 995,131 + -1.2 995,132 - +0.1 995,132 - +0.3 995,132 - -0.5 995,133 + -2.3 995,133 + +1.2 995,133 + +0.1 995,133 + +0.9 995,133 + -0.1 995,133 + -1.3 995,134 - -1.2 995,134 - -0.7 995,134 - -0.7 995,147 - +2.3 995,194 + -1.1 995,194 + +2.4 995,195 - -1.0 995,201 + IAI47_04720 0.10 -1.5 995,202 - IAI47_04720 0.10 -1.4 995,214 + IAI47_04720 0.12 -1.6 995,217 + IAI47_04720 0.12 -0.4 995,217 + IAI47_04720 0.12 +1.3 995,251 + IAI47_04720 0.16 -1.7 995,251 + IAI47_04720 0.16 -0.6 995,251 + IAI47_04720 0.16 -1.0 995,253 + IAI47_04720 0.16 +1.0 995,253 + IAI47_04720 0.16 +1.1 995,254 - IAI47_04720 0.16 +1.2 995,259 + IAI47_04720 0.16 +0.6 995,260 - IAI47_04720 0.17 -0.4 995,260 - IAI47_04720 0.17 -1.1 995,290 + IAI47_04720 0.20 +0.7 995,317 + IAI47_04720 0.23 +0.4 995,322 + IAI47_04720 0.23 -0.3 995,325 - IAI47_04720 0.24 -0.2 995,334 + IAI47_04720 0.25 -2.0 995,335 - IAI47_04720 0.25 -0.4 995,335 - IAI47_04720 0.25 -1.4 995,362 + IAI47_04720 0.28 -0.3 995,364 + IAI47_04720 0.28 +1.1 995,364 + IAI47_04720 0.28 +0.1 995,364 + IAI47_04720 0.28 -0.3 995,365 - IAI47_04720 0.28 +0.1 995,370 + IAI47_04720 0.28 -0.1 995,437 + IAI47_04720 0.36 -0.7 995,447 + IAI47_04720 0.37 +0.1 995,574 + IAI47_04720 0.51 +0.1 995,574 + IAI47_04720 0.51 -0.2 995,574 + IAI47_04720 0.51 +0.3 995,621 - IAI47_04720 0.56 -0.2 995,700 + IAI47_04720 0.64 -1.7 995,700 + IAI47_04720 0.64 -0.7 995,700 + IAI47_04720 0.64 -0.4 995,700 + IAI47_04720 0.64 +0.6 995,706 + IAI47_04720 0.65 -0.2 995,707 - IAI47_04720 0.65 -0.4 995,707 - IAI47_04720 0.65 +0.9 995,727 + IAI47_04720 0.67 -0.7 995,733 - IAI47_04720 0.68 +0.0 995,777 - IAI47_04720 0.73 -1.5 995,810 + IAI47_04720 0.76 +0.6 995,811 - IAI47_04720 0.76 -0.4 995,811 - IAI47_04720 0.76 -2.0 995,814 - IAI47_04720 0.77 +0.1 995,833 + IAI47_04720 0.79 +0.1 995,880 + IAI47_04720 0.84 +0.6 995,948 + -0.5 995,953 - -0.1 996,099 - IAI47_04725 0.18 +0.5 996,100 + IAI47_04725 0.18 +1.3 996,166 + IAI47_04725 0.32 -0.5 996,166 + IAI47_04725 0.32 -0.2 996,171 - 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Or see this region's nucleotide sequence