Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20965 and IAI47_20970 are separated by 105 nucleotides IAI47_20970 and IAI47_20975 are separated by 21 nucleotides IAI47_20975 and IAI47_20980 are separated by 213 nucleotides
IAI47_20965: IAI47_20965 - glutathione transferase GstA, at 441,556 to 442,161
_20965
IAI47_20970: IAI47_20970 - alpha/beta hydrolase, at 442,267 to 442,977
_20970
IAI47_20975: IAI47_20975 - cupin, at 442,999 to 443,310
_20975
IAI47_20980: IAI47_20980 - hypothetical protein, at 443,524 to 444,198
_20980
Position (kb) 442
443
444 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 442.024 kb on + strand, within IAI47_20965 at 442.025 kb on - strand, within IAI47_20965 at 442.025 kb on - strand, within IAI47_20965 at 442.025 kb on - strand, within IAI47_20965 at 442.025 kb on - strand, within IAI47_20965 at 442.025 kb on - strand, within IAI47_20965 at 442.030 kb on + strand, within IAI47_20965 at 442.030 kb on + strand, within IAI47_20965 at 442.030 kb on + strand, within IAI47_20965 at 442.030 kb on + strand, within IAI47_20965 at 442.030 kb on + strand, within IAI47_20965 at 442.031 kb on - strand, within IAI47_20965 at 442.031 kb on - strand, within IAI47_20965 at 442.031 kb on - strand, within IAI47_20965 at 442.139 kb on + strand at 442.140 kb on - strand at 442.159 kb on + strand at 442.227 kb on + strand at 442.227 kb on + strand at 442.227 kb on + strand at 442.227 kb on + strand at 442.228 kb on - strand at 442.228 kb on - strand at 442.228 kb on - strand at 442.267 kb on - strand at 442.334 kb on + strand at 442.334 kb on + strand at 442.334 kb on + strand at 442.335 kb on - strand at 442.335 kb on - strand at 442.337 kb on + strand at 442.437 kb on + strand, within IAI47_20970 at 442.437 kb on + strand, within IAI47_20970 at 442.438 kb on - strand, within IAI47_20970 at 442.438 kb on - strand, within IAI47_20970 at 442.438 kb on - strand, within IAI47_20970 at 442.463 kb on - strand, within IAI47_20970 at 442.552 kb on + strand, within IAI47_20970 at 442.553 kb on - strand, within IAI47_20970 at 442.553 kb on - strand, within IAI47_20970 at 442.670 kb on + strand, within IAI47_20970 at 442.671 kb on - strand, within IAI47_20970 at 442.688 kb on + strand, within IAI47_20970 at 442.710 kb on + strand, within IAI47_20970 at 442.803 kb on + strand, within IAI47_20970 at 442.803 kb on + strand, within IAI47_20970 at 442.803 kb on + strand, within IAI47_20970 at 442.803 kb on + strand, within IAI47_20970 at 442.803 kb on + strand, within IAI47_20970 at 442.804 kb on - strand, within IAI47_20970 at 442.804 kb on - strand, within IAI47_20970 at 442.827 kb on - strand, within IAI47_20970 at 442.827 kb on - strand, within IAI47_20970 at 442.846 kb on - strand, within IAI47_20970 at 442.869 kb on + strand, within IAI47_20970 at 442.869 kb on + strand, within IAI47_20970 at 442.869 kb on + strand, within IAI47_20970 at 442.870 kb on - strand, within IAI47_20970 at 442.870 kb on - strand, within IAI47_20970 at 442.870 kb on - strand, within IAI47_20970 at 442.892 kb on + strand, within IAI47_20970 at 442.892 kb on + strand, within IAI47_20970 at 442.892 kb on + strand, within IAI47_20970 at 442.892 kb on + strand, within IAI47_20970 at 442.892 kb on + strand, within IAI47_20970 at 442.892 kb on + strand, within IAI47_20970 at 442.892 kb on + strand, within IAI47_20970 at 442.892 kb on + strand, within IAI47_20970 at 442.892 kb on + strand, within IAI47_20970 at 442.892 kb on + strand, within IAI47_20970 at 442.893 kb on - strand, within IAI47_20970 at 442.893 kb on - strand, within IAI47_20970 at 442.893 kb on - strand, within IAI47_20970 at 442.893 kb on - strand, within IAI47_20970 at 442.895 kb on + strand, within IAI47_20970 at 442.895 kb on + strand, within IAI47_20970 at 442.895 kb on + strand, within IAI47_20970 at 442.895 kb on + strand, within IAI47_20970 at 442.895 kb on + strand, within IAI47_20970 at 442.895 kb on + strand, within IAI47_20970 at 442.895 kb on + strand, within IAI47_20970 at 442.895 kb on + strand, within IAI47_20970 at 442.895 kb on + strand, within IAI47_20970 at 442.896 kb on - strand, within IAI47_20970 at 442.896 kb on - strand, within IAI47_20970 at 442.896 kb on - strand, within IAI47_20970 at 442.896 kb on - strand, within IAI47_20970 at 442.896 kb on - strand, within IAI47_20970 at 442.896 kb on - strand, within IAI47_20970 at 442.896 kb on - strand, within IAI47_20970 at 442.899 kb on + strand, within IAI47_20970 at 442.899 kb on + strand, within IAI47_20970 at 442.900 kb on - strand, within IAI47_20970 at 442.900 kb on - strand, within IAI47_20970 at 442.900 kb on - strand, within IAI47_20970 at 442.947 kb on - strand at 442.966 kb on + strand at 442.967 kb on - strand at 443.000 kb on + strand at 443.081 kb on + strand, within IAI47_20975 at 443.089 kb on - strand, within IAI47_20975 at 443.089 kb on - strand, within IAI47_20975 at 443.089 kb on - strand, within IAI47_20975 at 443.116 kb on - strand, within IAI47_20975 at 443.133 kb on + strand, within IAI47_20975 at 443.133 kb on + strand, within IAI47_20975 at 443.133 kb on + strand, within IAI47_20975 at 443.133 kb on + strand, within IAI47_20975 at 443.133 kb on + strand, within IAI47_20975 at 443.134 kb on - strand, within IAI47_20975 at 443.134 kb on - strand, within IAI47_20975 at 443.135 kb on + strand, within IAI47_20975 at 443.135 kb on + strand, within IAI47_20975 at 443.135 kb on + strand, within IAI47_20975 at 443.135 kb on + strand, within IAI47_20975 at 443.136 kb on - strand, within IAI47_20975 at 443.136 kb on - strand, within IAI47_20975 at 443.136 kb on - strand, within IAI47_20975 at 443.136 kb on - strand, within IAI47_20975 at 443.201 kb on + strand, within IAI47_20975 at 443.201 kb on + strand, within IAI47_20975 at 443.201 kb on + strand, within IAI47_20975 at 443.202 kb on - strand, within IAI47_20975 at 443.202 kb on - strand, within IAI47_20975 at 443.238 kb on + strand, within IAI47_20975 at 443.239 kb on - strand, within IAI47_20975 at 443.239 kb on - strand, within IAI47_20975 at 443.239 kb on - strand, within IAI47_20975 at 443.239 kb on - strand, within IAI47_20975 at 443.253 kb on + strand, within IAI47_20975 at 443.253 kb on + strand, within IAI47_20975 at 443.253 kb on + strand, within IAI47_20975 at 443.253 kb on + strand, within IAI47_20975 at 443.254 kb on - strand, within IAI47_20975 at 443.254 kb on - strand, within IAI47_20975 at 443.337 kb on - strand at 443.337 kb on - strand at 443.349 kb on - strand at 443.371 kb on + strand at 443.372 kb on - strand at 443.372 kb on - strand at 443.372 kb on - strand at 443.372 kb on - strand at 443.384 kb on + strand at 443.384 kb on + strand at 443.385 kb on - strand at 443.385 kb on - strand at 443.385 kb on - strand at 443.419 kb on - strand at 443.435 kb on + strand at 443.439 kb on + strand at 443.439 kb on + strand at 443.440 kb on - strand at 443.440 kb on - strand at 443.451 kb on + strand at 443.452 kb on - strand at 443.452 kb on - strand at 443.457 kb on - strand at 443.475 kb on - strand at 443.475 kb on - strand at 443.482 kb on + strand at 443.483 kb on - strand at 443.502 kb on + strand at 443.503 kb on - strand at 443.542 kb on + strand at 443.543 kb on - strand at 443.602 kb on - strand, within IAI47_20980 at 443.602 kb on - strand, within IAI47_20980 at 443.604 kb on + strand, within IAI47_20980 at 443.604 kb on + strand, within IAI47_20980 at 443.604 kb on + strand, within IAI47_20980 at 443.604 kb on + strand, within IAI47_20980 at 443.604 kb on + strand, within IAI47_20980 at 443.605 kb on - strand, within IAI47_20980 at 443.605 kb on - strand, within IAI47_20980 at 443.605 kb on - strand, within IAI47_20980 at 443.605 kb on - strand, within IAI47_20980 at 443.617 kb on + strand, within IAI47_20980 at 443.620 kb on + strand, within IAI47_20980 at 443.620 kb on + strand, within IAI47_20980 at 443.621 kb on - strand, within IAI47_20980 at 443.654 kb on + strand, within IAI47_20980 at 443.655 kb on - strand, within IAI47_20980 at 443.655 kb on - strand, within IAI47_20980 at 443.689 kb on - strand, within IAI47_20980 at 443.760 kb on + strand, within IAI47_20980 at 443.761 kb on - strand, within IAI47_20980 at 443.787 kb on + strand, within IAI47_20980 at 443.868 kb on + strand, within IAI47_20980 at 443.869 kb on - strand, within IAI47_20980 at 443.870 kb on + strand, within IAI47_20980 at 443.932 kb on - strand, within IAI47_20980 at 443.932 kb on - strand, within IAI47_20980 at 443.932 kb on - strand, within IAI47_20980 at 444.023 kb on + strand, within IAI47_20980 at 444.023 kb on + strand, within IAI47_20980 at 444.024 kb on - strand, within IAI47_20980 at 444.075 kb on - strand, within IAI47_20980 at 444.094 kb on + strand, within IAI47_20980 at 444.094 kb on + strand, within IAI47_20980 at 444.095 kb on - strand, within IAI47_20980 at 444.097 kb on - strand, within IAI47_20980 at 444.097 kb on - strand, within IAI47_20980 at 444.133 kb on - strand at 444.143 kb on + strand at 444.163 kb on + strand at 444.163 kb on + strand at 444.163 kb on + strand at 444.163 kb on + strand at 444.164 kb on - strand at 444.164 kb on - strand at 444.164 kb on - strand at 444.164 kb on - strand at 444.164 kb on - strand at 444.164 kb on - strand at 444.164 kb on - strand at 444.164 kb on - strand at 444.165 kb on + strand at 444.165 kb on + strand at 444.165 kb on + strand at 444.165 kb on + strand at 444.166 kb on - strand at 444.166 kb on - strand at 444.166 kb on - strand at 444.176 kb on + strand at 444.176 kb on + strand at 444.176 kb on + strand at 444.176 kb on + strand at 444.176 kb on + strand at 444.177 kb on - strand at 444.177 kb on - strand at 444.177 kb on - strand at 444.177 kb on - strand at 444.177 kb on - strand at 444.177 kb on - strand at 444.177 kb on - strand at 444.177 kb on - strand at 444.196 kb on + strand at 444.196 kb on + strand at 444.206 kb on + strand at 444.206 kb on + strand at 444.207 kb on - strand at 444.207 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 442,024 + IAI47_20965 0.77 +1.0 442,025 - IAI47_20965 0.77 -0.6 442,025 - IAI47_20965 0.77 +1.7 442,025 - IAI47_20965 0.77 -1.2 442,025 - IAI47_20965 0.77 +1.8 442,025 - IAI47_20965 0.77 +0.2 442,030 + IAI47_20965 0.78 +0.7 442,030 + IAI47_20965 0.78 -1.9 442,030 + IAI47_20965 0.78 +2.1 442,030 + IAI47_20965 0.78 +0.1 442,030 + IAI47_20965 0.78 +0.8 442,031 - IAI47_20965 0.78 +0.7 442,031 - IAI47_20965 0.78 -0.2 442,031 - IAI47_20965 0.78 -0.6 442,139 + -0.4 442,140 - -0.7 442,159 + +0.3 442,227 + -0.3 442,227 + +0.0 442,227 + -0.2 442,227 + -0.7 442,228 - +0.4 442,228 - +1.1 442,228 - +0.1 442,267 - -1.0 442,334 + +1.3 442,334 + -1.2 442,334 + +0.5 442,335 - +0.1 442,335 - -1.2 442,337 + +1.0 442,437 + IAI47_20970 0.24 -0.4 442,437 + IAI47_20970 0.24 +0.6 442,438 - IAI47_20970 0.24 +0.3 442,438 - IAI47_20970 0.24 -0.7 442,438 - IAI47_20970 0.24 +1.3 442,463 - IAI47_20970 0.28 -1.3 442,552 + IAI47_20970 0.40 -1.3 442,553 - IAI47_20970 0.40 -1.3 442,553 - IAI47_20970 0.40 -1.1 442,670 + IAI47_20970 0.57 -1.9 442,671 - IAI47_20970 0.57 -0.5 442,688 + IAI47_20970 0.59 +2.5 442,710 + IAI47_20970 0.62 -0.9 442,803 + IAI47_20970 0.75 -0.7 442,803 + IAI47_20970 0.75 +1.6 442,803 + IAI47_20970 0.75 -0.8 442,803 + IAI47_20970 0.75 -0.0 442,803 + IAI47_20970 0.75 -0.6 442,804 - IAI47_20970 0.76 -0.9 442,804 - IAI47_20970 0.76 +0.4 442,827 - IAI47_20970 0.79 +0.3 442,827 - IAI47_20970 0.79 -0.3 442,846 - IAI47_20970 0.81 -0.8 442,869 + IAI47_20970 0.85 +0.0 442,869 + IAI47_20970 0.85 +0.1 442,869 + IAI47_20970 0.85 -0.1 442,870 - IAI47_20970 0.85 -0.7 442,870 - IAI47_20970 0.85 -0.6 442,870 - IAI47_20970 0.85 -0.5 442,892 + IAI47_20970 0.88 -0.3 442,892 + IAI47_20970 0.88 -1.3 442,892 + IAI47_20970 0.88 -0.2 442,892 + IAI47_20970 0.88 +0.4 442,892 + IAI47_20970 0.88 -1.0 442,892 + IAI47_20970 0.88 +1.0 442,892 + IAI47_20970 0.88 -0.3 442,892 + IAI47_20970 0.88 -0.1 442,892 + IAI47_20970 0.88 +1.4 442,892 + IAI47_20970 0.88 -0.6 442,893 - IAI47_20970 0.88 -0.9 442,893 - IAI47_20970 0.88 +2.0 442,893 - IAI47_20970 0.88 +1.0 442,893 - IAI47_20970 0.88 +0.2 442,895 + IAI47_20970 0.88 +0.4 442,895 + IAI47_20970 0.88 -0.3 442,895 + IAI47_20970 0.88 -1.3 442,895 + IAI47_20970 0.88 +0.3 442,895 + IAI47_20970 0.88 -1.0 442,895 + IAI47_20970 0.88 +0.3 442,895 + IAI47_20970 0.88 +1.6 442,895 + IAI47_20970 0.88 +0.5 442,895 + IAI47_20970 0.88 -0.3 442,896 - IAI47_20970 0.88 +0.0 442,896 - IAI47_20970 0.88 +0.7 442,896 - IAI47_20970 0.88 +2.1 442,896 - IAI47_20970 0.88 +0.1 442,896 - IAI47_20970 0.88 -0.6 442,896 - IAI47_20970 0.88 +0.4 442,896 - IAI47_20970 0.88 +0.5 442,899 + IAI47_20970 0.89 -0.7 442,899 + IAI47_20970 0.89 +1.8 442,900 - IAI47_20970 0.89 -2.0 442,900 - IAI47_20970 0.89 -0.0 442,900 - IAI47_20970 0.89 +0.0 442,947 - +0.8 442,966 + +0.8 442,967 - -0.5 443,000 + -0.6 443,081 + IAI47_20975 0.26 +0.4 443,089 - IAI47_20975 0.29 -0.9 443,089 - IAI47_20975 0.29 +1.4 443,089 - IAI47_20975 0.29 +0.3 443,116 - IAI47_20975 0.38 +0.1 443,133 + IAI47_20975 0.43 +0.0 443,133 + IAI47_20975 0.43 +0.8 443,133 + IAI47_20975 0.43 -0.5 443,133 + IAI47_20975 0.43 -0.9 443,133 + IAI47_20975 0.43 -0.6 443,134 - IAI47_20975 0.43 -0.6 443,134 - IAI47_20975 0.43 +0.4 443,135 + IAI47_20975 0.44 -1.3 443,135 + IAI47_20975 0.44 -2.0 443,135 + IAI47_20975 0.44 +0.3 443,135 + IAI47_20975 0.44 -0.8 443,136 - IAI47_20975 0.44 -0.4 443,136 - IAI47_20975 0.44 +1.0 443,136 - IAI47_20975 0.44 -0.6 443,136 - IAI47_20975 0.44 -1.1 443,201 + IAI47_20975 0.65 -0.7 443,201 + IAI47_20975 0.65 -1.5 443,201 + IAI47_20975 0.65 -0.7 443,202 - IAI47_20975 0.65 +0.1 443,202 - IAI47_20975 0.65 +0.1 443,238 + IAI47_20975 0.77 -0.2 443,239 - IAI47_20975 0.77 -0.8 443,239 - IAI47_20975 0.77 -0.7 443,239 - IAI47_20975 0.77 +0.1 443,239 - IAI47_20975 0.77 -1.7 443,253 + IAI47_20975 0.81 +2.3 443,253 + IAI47_20975 0.81 -0.4 443,253 + IAI47_20975 0.81 -0.4 443,253 + IAI47_20975 0.81 +1.4 443,254 - IAI47_20975 0.82 +0.2 443,254 - IAI47_20975 0.82 -0.9 443,337 - -1.8 443,337 - +1.0 443,349 - +0.4 443,371 + -0.4 443,372 - +0.2 443,372 - +1.3 443,372 - +0.6 443,372 - -1.1 443,384 + +1.5 443,384 + +0.2 443,385 - +0.9 443,385 - -0.7 443,385 - +1.7 443,419 - -1.6 443,435 + -1.5 443,439 + -2.3 443,439 + -1.9 443,440 - +0.5 443,440 - +1.4 443,451 + +0.2 443,452 - -1.2 443,452 - -0.3 443,457 - +0.1 443,475 - +0.3 443,475 - -0.3 443,482 + -0.2 443,483 - -0.4 443,502 + -0.2 443,503 - -0.4 443,542 + +0.6 443,543 - -0.4 443,602 - IAI47_20980 0.12 +0.0 443,602 - IAI47_20980 0.12 -0.4 443,604 + IAI47_20980 0.12 -0.4 443,604 + IAI47_20980 0.12 +0.0 443,604 + IAI47_20980 0.12 +1.7 443,604 + IAI47_20980 0.12 +2.1 443,604 + IAI47_20980 0.12 -0.3 443,605 - IAI47_20980 0.12 -0.9 443,605 - IAI47_20980 0.12 -0.9 443,605 - IAI47_20980 0.12 +0.7 443,605 - IAI47_20980 0.12 -0.2 443,617 + IAI47_20980 0.14 +1.1 443,620 + IAI47_20980 0.14 +1.6 443,620 + IAI47_20980 0.14 +0.1 443,621 - IAI47_20980 0.14 +0.3 443,654 + IAI47_20980 0.19 -0.3 443,655 - IAI47_20980 0.19 +0.6 443,655 - IAI47_20980 0.19 +2.1 443,689 - IAI47_20980 0.24 +0.1 443,760 + IAI47_20980 0.35 -0.9 443,761 - IAI47_20980 0.35 +0.8 443,787 + IAI47_20980 0.39 -2.9 443,868 + IAI47_20980 0.51 +0.7 443,869 - IAI47_20980 0.51 +1.3 443,870 + IAI47_20980 0.51 +0.3 443,932 - IAI47_20980 0.60 -0.8 443,932 - IAI47_20980 0.60 +1.3 443,932 - IAI47_20980 0.60 +0.9 444,023 + IAI47_20980 0.74 +1.1 444,023 + IAI47_20980 0.74 +1.1 444,024 - IAI47_20980 0.74 +0.4 444,075 - IAI47_20980 0.82 +0.5 444,094 + IAI47_20980 0.84 -1.0 444,094 + IAI47_20980 0.84 -1.1 444,095 - IAI47_20980 0.85 -0.8 444,097 - IAI47_20980 0.85 +1.8 444,097 - IAI47_20980 0.85 +0.7 444,133 - -0.6 444,143 + +0.1 444,163 + +0.3 444,163 + +0.3 444,163 + -0.5 444,163 + +0.9 444,164 - +1.0 444,164 - +0.0 444,164 - -2.1 444,164 - +2.0 444,164 - -0.3 444,164 - +0.4 444,164 - -1.7 444,164 - +0.0 444,165 + +1.4 444,165 + -1.3 444,165 + +0.3 444,165 + +0.7 444,166 - -1.0 444,166 - -0.8 444,166 - -1.8 444,176 + -0.4 444,176 + +0.1 444,176 + -1.4 444,176 + -1.8 444,176 + +0.3 444,177 - +0.8 444,177 - -0.1 444,177 - +1.3 444,177 - +3.0 444,177 - -0.1 444,177 - -1.7 444,177 - -0.7 444,177 - -0.5 444,196 + -0.3 444,196 + -0.3 444,206 + +1.7 444,206 + +0.1 444,207 - -0.4 444,207 - -2.6
Or see this region's nucleotide sequence