Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20010 and IAI47_20015 are separated by 417 nucleotides IAI47_20015 and IAI47_20020 are separated by 19 nucleotides
IAI47_20010: IAI47_20010 - GntR family transcriptional regulator, at 234,187 to 234,921
_20010
IAI47_20015: IAI47_20015 - MsnO8 family LLM class oxidoreductase, at 235,339 to 236,337
_20015
IAI47_20020: IAI47_20020 - amidohydrolase, at 236,357 to 237,508
_20020
Position (kb) 235
236
237 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 234.345 kb on + strand, within IAI47_20010 at 234.345 kb on + strand, within IAI47_20010 at 234.345 kb on + strand, within IAI47_20010 at 234.345 kb on + strand, within IAI47_20010 at 234.345 kb on + strand, within IAI47_20010 at 234.345 kb on + strand, within IAI47_20010 at 234.345 kb on + strand, within IAI47_20010 at 234.346 kb on - strand, within IAI47_20010 at 234.346 kb on - strand, within IAI47_20010 at 234.346 kb on - strand at 234.346 kb on - strand, within IAI47_20010 at 234.346 kb on - strand, within IAI47_20010 at 234.353 kb on + strand, within IAI47_20010 at 234.368 kb on + strand, within IAI47_20010 at 234.368 kb on + strand, within IAI47_20010 at 234.368 kb on + strand, within IAI47_20010 at 234.369 kb on - strand, within IAI47_20010 at 234.369 kb on - strand, within IAI47_20010 at 234.371 kb on - strand, within IAI47_20010 at 234.378 kb on - strand, within IAI47_20010 at 234.413 kb on + strand, within IAI47_20010 at 234.413 kb on + strand, within IAI47_20010 at 234.414 kb on - strand, within IAI47_20010 at 234.414 kb on - strand, within IAI47_20010 at 234.417 kb on + strand, within IAI47_20010 at 234.418 kb on - strand, within IAI47_20010 at 234.511 kb on - strand, within IAI47_20010 at 234.627 kb on + strand, within IAI47_20010 at 234.628 kb on - strand, within IAI47_20010 at 234.628 kb on - strand, within IAI47_20010 at 234.676 kb on + strand, within IAI47_20010 at 234.688 kb on + strand, within IAI47_20010 at 234.688 kb on + strand, within IAI47_20010 at 234.688 kb on + strand, within IAI47_20010 at 234.688 kb on + strand, within IAI47_20010 at 234.688 kb on + strand, within IAI47_20010 at 234.688 kb on + strand, within IAI47_20010 at 234.688 kb on + strand, within IAI47_20010 at 234.689 kb on - strand, within IAI47_20010 at 234.689 kb on - strand, within IAI47_20010 at 234.689 kb on - strand, within IAI47_20010 at 234.694 kb on + strand, within IAI47_20010 at 234.694 kb on + strand, within IAI47_20010 at 234.694 kb on + strand, within IAI47_20010 at 234.695 kb on - strand, within IAI47_20010 at 234.695 kb on - strand, within IAI47_20010 at 234.696 kb on + strand, within IAI47_20010 at 234.696 kb on + strand, within IAI47_20010 at 234.697 kb on - strand, within IAI47_20010 at 234.715 kb on - strand, within IAI47_20010 at 234.715 kb on - strand, within IAI47_20010 at 234.765 kb on + strand, within IAI47_20010 at 234.766 kb on - strand, within IAI47_20010 at 234.777 kb on + strand, within IAI47_20010 at 234.777 kb on + strand, within IAI47_20010 at 234.777 kb on + strand, within IAI47_20010 at 234.778 kb on - strand, within IAI47_20010 at 234.778 kb on - strand, within IAI47_20010 at 234.782 kb on + strand, within IAI47_20010 at 234.787 kb on - strand, within IAI47_20010 at 234.787 kb on - strand, within IAI47_20010 at 234.832 kb on + strand, within IAI47_20010 at 234.919 kb on + strand at 234.924 kb on - strand at 234.929 kb on - strand at 234.944 kb on + strand at 234.944 kb on + strand at 234.944 kb on + strand at 234.945 kb on - strand at 234.969 kb on + strand at 234.969 kb on + strand at 234.969 kb on + strand at 234.969 kb on + strand at 234.969 kb on + strand at 234.970 kb on - strand at 234.970 kb on - strand at 234.975 kb on + strand at 234.975 kb on + strand at 234.976 kb on - strand at 234.979 kb on + strand at 234.980 kb on - strand at 235.038 kb on - strand at 235.098 kb on + strand at 235.099 kb on - strand at 235.099 kb on - strand at 235.099 kb on - strand at 235.156 kb on + strand at 235.157 kb on - strand at 235.183 kb on + strand at 235.183 kb on + strand at 235.184 kb on - strand at 235.184 kb on - strand at 235.184 kb on - strand at 235.187 kb on - strand at 235.187 kb on - strand at 235.220 kb on + strand at 235.220 kb on + strand at 235.255 kb on - strand at 235.261 kb on + strand at 235.268 kb on + strand at 235.269 kb on + strand at 235.269 kb on + strand at 235.269 kb on + strand at 235.280 kb on - strand at 235.285 kb on + strand at 235.322 kb on + strand at 235.346 kb on - strand at 235.361 kb on + strand at 235.365 kb on + strand at 235.365 kb on + strand at 235.365 kb on + strand at 235.365 kb on + strand at 235.366 kb on - strand at 235.366 kb on - strand at 235.366 kb on - strand at 235.366 kb on - strand at 235.450 kb on + strand, within IAI47_20015 at 235.450 kb on + strand, within IAI47_20015 at 235.517 kb on + strand, within IAI47_20015 at 235.517 kb on + strand, within IAI47_20015 at 235.518 kb on - strand, within IAI47_20015 at 235.518 kb on - strand, within IAI47_20015 at 235.518 kb on - strand, within IAI47_20015 at 235.518 kb on - strand, within IAI47_20015 at 235.548 kb on + strand, within IAI47_20015 at 235.548 kb on + strand, within IAI47_20015 at 235.585 kb on + strand, within IAI47_20015 at 235.585 kb on + strand, within IAI47_20015 at 235.585 kb on + strand, within IAI47_20015 at 235.586 kb on - strand, within IAI47_20015 at 235.596 kb on + strand, within IAI47_20015 at 235.596 kb on + strand, within IAI47_20015 at 235.596 kb on + strand, within IAI47_20015 at 235.596 kb on + strand, within IAI47_20015 at 235.597 kb on - strand, within IAI47_20015 at 235.597 kb on - strand, within IAI47_20015 at 235.597 kb on - strand, within IAI47_20015 at 235.597 kb on - strand, within IAI47_20015 at 235.597 kb on - strand, within IAI47_20015 at 235.644 kb on + strand, within IAI47_20015 at 235.644 kb on + strand, within IAI47_20015 at 235.659 kb on - strand, within IAI47_20015 at 235.659 kb on - strand, within IAI47_20015 at 235.680 kb on - strand, within IAI47_20015 at 235.741 kb on - strand, within IAI47_20015 at 235.741 kb on - strand, within IAI47_20015 at 235.761 kb on + strand, within IAI47_20015 at 235.762 kb on - strand, within IAI47_20015 at 235.787 kb on - strand, within IAI47_20015 at 235.806 kb on - strand, within IAI47_20015 at 235.938 kb on - strand, within IAI47_20015 at 235.952 kb on + strand, within IAI47_20015 at 235.952 kb on + strand, within IAI47_20015 at 235.953 kb on - strand, within IAI47_20015 at 235.956 kb on + strand, within IAI47_20015 at 235.957 kb on - strand, within IAI47_20015 at 236.000 kb on + strand, within IAI47_20015 at 236.001 kb on - strand, within IAI47_20015 at 236.001 kb on - strand, within IAI47_20015 at 236.125 kb on + strand, within IAI47_20015 at 236.125 kb on + strand, within IAI47_20015 at 236.125 kb on + strand, within IAI47_20015 at 236.125 kb on + strand, within IAI47_20015 at 236.125 kb on + strand, within IAI47_20015 at 236.125 kb on + strand, within IAI47_20015 at 236.126 kb on - strand, within IAI47_20015 at 236.126 kb on - strand, within IAI47_20015 at 236.152 kb on + strand, within IAI47_20015 at 236.152 kb on + strand, within IAI47_20015 at 236.159 kb on + strand, within IAI47_20015 at 236.160 kb on - strand, within IAI47_20015 at 236.160 kb on - strand, within IAI47_20015 at 236.183 kb on + strand, within IAI47_20015 at 236.183 kb on + strand, within IAI47_20015 at 236.183 kb on + strand, within IAI47_20015 at 236.184 kb on - strand, within IAI47_20015 at 236.232 kb on + strand, within IAI47_20015 at 236.233 kb on - strand, within IAI47_20015 at 236.233 kb on - strand, within IAI47_20015 at 236.313 kb on + strand at 236.401 kb on + strand at 236.401 kb on + strand at 236.401 kb on + strand at 236.401 kb on + strand at 236.401 kb on + strand at 236.401 kb on + strand at 236.402 kb on - strand at 236.402 kb on - strand at 236.402 kb on - strand at 236.402 kb on - strand at 236.402 kb on - strand at 236.402 kb on - strand at 236.700 kb on + strand, within IAI47_20020 at 236.700 kb on + strand, within IAI47_20020 at 236.701 kb on - strand, within IAI47_20020 at 236.701 kb on - strand, within IAI47_20020 at 236.712 kb on + strand, within IAI47_20020 at 236.716 kb on + strand, within IAI47_20020 at 236.716 kb on + strand, within IAI47_20020 at 236.716 kb on + strand, within IAI47_20020 at 236.716 kb on + strand, within IAI47_20020 at 236.716 kb on + strand, within IAI47_20020 at 236.717 kb on - strand, within IAI47_20020 at 236.717 kb on - strand, within IAI47_20020 at 236.717 kb on - strand, within IAI47_20020 at 236.717 kb on - strand, within IAI47_20020 at 236.717 kb on - strand, within IAI47_20020 at 236.764 kb on + strand, within IAI47_20020 at 236.764 kb on + strand, within IAI47_20020 at 236.765 kb on - strand, within IAI47_20020 at 236.778 kb on + strand, within IAI47_20020 at 236.779 kb on - strand, within IAI47_20020 at 236.779 kb on - strand, within IAI47_20020 at 236.790 kb on + strand, within IAI47_20020 at 236.790 kb on + strand, within IAI47_20020 at 236.790 kb on + strand, within IAI47_20020 at 236.790 kb on + strand, within IAI47_20020 at 236.791 kb on - strand, within IAI47_20020 at 236.791 kb on - strand, within IAI47_20020 at 236.864 kb on + strand, within IAI47_20020 at 236.872 kb on + strand, within IAI47_20020 at 236.872 kb on + strand, within IAI47_20020 at 236.884 kb on + strand, within IAI47_20020 at 236.884 kb on + strand, within IAI47_20020 at 236.888 kb on - strand, within IAI47_20020 at 237.028 kb on + strand, within IAI47_20020 at 237.028 kb on + strand, within IAI47_20020 at 237.029 kb on - strand, within IAI47_20020 at 237.031 kb on + strand, within IAI47_20020 at 237.032 kb on - strand, within IAI47_20020 at 237.032 kb on - strand, within IAI47_20020 at 237.032 kb on - strand, within IAI47_20020 at 237.032 kb on - strand, within IAI47_20020 at 237.032 kb on - strand, within IAI47_20020 at 237.066 kb on + strand, within IAI47_20020 at 237.111 kb on + strand, within IAI47_20020 at 237.112 kb on - strand, within IAI47_20020 at 237.112 kb on - strand, within IAI47_20020 at 237.326 kb on + strand, within IAI47_20020 at 237.326 kb on + strand, within IAI47_20020
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 234,345 + IAI47_20010 0.21 +1.6 234,345 + IAI47_20010 0.21 +0.8 234,345 + IAI47_20010 0.21 -0.1 234,345 + IAI47_20010 0.21 +1.5 234,345 + IAI47_20010 0.21 -0.8 234,345 + IAI47_20010 0.21 -0.6 234,345 + IAI47_20010 0.21 +0.9 234,346 - IAI47_20010 0.22 +0.4 234,346 - IAI47_20010 0.22 +0.1 234,346 - +0.2 234,346 - IAI47_20010 0.22 -1.2 234,346 - IAI47_20010 0.22 -0.2 234,353 + IAI47_20010 0.23 +1.4 234,368 + IAI47_20010 0.25 +0.1 234,368 + IAI47_20010 0.25 -0.8 234,368 + IAI47_20010 0.25 -0.0 234,369 - IAI47_20010 0.25 +0.6 234,369 - IAI47_20010 0.25 -1.0 234,371 - IAI47_20010 0.25 -1.1 234,378 - IAI47_20010 0.26 +0.3 234,413 + IAI47_20010 0.31 -2.1 234,413 + IAI47_20010 0.31 +1.6 234,414 - IAI47_20010 0.31 +0.0 234,414 - IAI47_20010 0.31 -0.6 234,417 + IAI47_20010 0.31 -1.0 234,418 - IAI47_20010 0.31 +0.2 234,511 - IAI47_20010 0.44 -1.5 234,627 + IAI47_20010 0.60 +1.5 234,628 - IAI47_20010 0.60 -0.7 234,628 - IAI47_20010 0.60 -0.3 234,676 + IAI47_20010 0.67 +1.9 234,688 + IAI47_20010 0.68 +1.6 234,688 + IAI47_20010 0.68 -1.0 234,688 + IAI47_20010 0.68 -0.7 234,688 + IAI47_20010 0.68 +0.4 234,688 + IAI47_20010 0.68 -0.9 234,688 + IAI47_20010 0.68 +1.2 234,688 + IAI47_20010 0.68 -0.3 234,689 - IAI47_20010 0.68 -0.6 234,689 - IAI47_20010 0.68 -0.1 234,689 - IAI47_20010 0.68 +0.3 234,694 + IAI47_20010 0.69 +0.7 234,694 + IAI47_20010 0.69 +1.0 234,694 + IAI47_20010 0.69 -1.5 234,695 - IAI47_20010 0.69 +0.2 234,695 - IAI47_20010 0.69 -2.3 234,696 + IAI47_20010 0.69 +0.6 234,696 + IAI47_20010 0.69 +1.7 234,697 - IAI47_20010 0.69 +0.9 234,715 - IAI47_20010 0.72 +1.3 234,715 - IAI47_20010 0.72 -0.3 234,765 + IAI47_20010 0.79 +0.2 234,766 - IAI47_20010 0.79 -0.4 234,777 + IAI47_20010 0.80 +0.0 234,777 + IAI47_20010 0.80 +0.9 234,777 + IAI47_20010 0.80 -0.1 234,778 - IAI47_20010 0.80 +1.9 234,778 - IAI47_20010 0.80 -0.1 234,782 + IAI47_20010 0.81 +0.6 234,787 - IAI47_20010 0.82 -1.3 234,787 - IAI47_20010 0.82 -0.4 234,832 + IAI47_20010 0.88 -1.0 234,919 + +1.2 234,924 - -2.9 234,929 - -1.5 234,944 + +1.7 234,944 + +0.7 234,944 + -2.3 234,945 - +0.1 234,969 + +0.1 234,969 + -2.4 234,969 + +1.3 234,969 + -0.5 234,969 + -1.4 234,970 - -1.4 234,970 - -1.2 234,975 + +0.3 234,975 + -0.1 234,976 - +0.1 234,979 + -0.4 234,980 - -1.4 235,038 - -0.3 235,098 + -1.0 235,099 - +1.2 235,099 - -0.7 235,099 - -1.9 235,156 + +0.9 235,157 - +1.7 235,183 + +6.5 235,183 + -0.8 235,184 - +1.1 235,184 - +1.2 235,184 - -0.2 235,187 - +0.0 235,187 - +1.4 235,220 + -0.0 235,220 + +1.2 235,255 - -0.4 235,261 + -0.9 235,268 + +0.3 235,269 + +0.7 235,269 + +1.5 235,269 + -0.9 235,280 - -1.5 235,285 + +0.6 235,322 + +0.5 235,346 - +0.4 235,361 + +0.7 235,365 + -0.0 235,365 + +0.7 235,365 + -0.0 235,365 + -0.3 235,366 - +1.7 235,366 - +1.7 235,366 - +0.5 235,366 - +1.3 235,450 + IAI47_20015 0.11 -0.9 235,450 + IAI47_20015 0.11 +0.7 235,517 + IAI47_20015 0.18 -1.1 235,517 + IAI47_20015 0.18 +2.0 235,518 - IAI47_20015 0.18 -0.5 235,518 - IAI47_20015 0.18 +1.3 235,518 - IAI47_20015 0.18 -1.0 235,518 - IAI47_20015 0.18 -0.1 235,548 + IAI47_20015 0.21 +1.6 235,548 + IAI47_20015 0.21 -0.3 235,585 + IAI47_20015 0.25 -1.3 235,585 + IAI47_20015 0.25 +0.1 235,585 + IAI47_20015 0.25 -1.2 235,586 - IAI47_20015 0.25 +1.5 235,596 + IAI47_20015 0.26 -0.3 235,596 + IAI47_20015 0.26 +3.4 235,596 + IAI47_20015 0.26 +0.1 235,596 + IAI47_20015 0.26 +1.5 235,597 - IAI47_20015 0.26 +0.9 235,597 - IAI47_20015 0.26 -0.8 235,597 - IAI47_20015 0.26 +0.3 235,597 - IAI47_20015 0.26 +1.6 235,597 - IAI47_20015 0.26 +1.0 235,644 + IAI47_20015 0.31 +0.3 235,644 + IAI47_20015 0.31 +0.6 235,659 - IAI47_20015 0.32 +0.3 235,659 - IAI47_20015 0.32 -0.7 235,680 - IAI47_20015 0.34 +1.7 235,741 - IAI47_20015 0.40 +1.1 235,741 - IAI47_20015 0.40 -0.8 235,761 + IAI47_20015 0.42 -0.4 235,762 - IAI47_20015 0.42 +0.7 235,787 - IAI47_20015 0.45 +0.0 235,806 - IAI47_20015 0.47 -0.4 235,938 - IAI47_20015 0.60 -1.9 235,952 + IAI47_20015 0.61 -0.8 235,952 + IAI47_20015 0.61 -0.9 235,953 - IAI47_20015 0.61 +0.7 235,956 + IAI47_20015 0.62 -0.8 235,957 - IAI47_20015 0.62 -0.1 236,000 + IAI47_20015 0.66 -0.7 236,001 - IAI47_20015 0.66 +1.3 236,001 - IAI47_20015 0.66 -1.1 236,125 + IAI47_20015 0.79 -1.3 236,125 + IAI47_20015 0.79 -0.8 236,125 + IAI47_20015 0.79 -0.3 236,125 + IAI47_20015 0.79 +0.7 236,125 + IAI47_20015 0.79 +0.1 236,125 + IAI47_20015 0.79 +0.1 236,126 - IAI47_20015 0.79 -1.1 236,126 - IAI47_20015 0.79 +2.3 236,152 + IAI47_20015 0.81 -0.9 236,152 + IAI47_20015 0.81 +2.3 236,159 + IAI47_20015 0.82 -1.2 236,160 - IAI47_20015 0.82 +0.4 236,160 - IAI47_20015 0.82 +0.8 236,183 + IAI47_20015 0.84 +1.3 236,183 + IAI47_20015 0.84 -0.8 236,183 + IAI47_20015 0.84 +0.8 236,184 - IAI47_20015 0.85 -1.3 236,232 + IAI47_20015 0.89 +2.3 236,233 - IAI47_20015 0.89 +0.2 236,233 - IAI47_20015 0.89 +0.1 236,313 + +0.4 236,401 + +1.7 236,401 + +0.5 236,401 + -1.8 236,401 + +0.2 236,401 + +1.3 236,401 + +0.1 236,402 - +0.1 236,402 - +1.1 236,402 - +2.5 236,402 - -1.2 236,402 - -0.3 236,402 - +1.4 236,700 + IAI47_20020 0.30 +1.8 236,700 + IAI47_20020 0.30 -1.2 236,701 - IAI47_20020 0.30 +0.9 236,701 - IAI47_20020 0.30 +2.0 236,712 + IAI47_20020 0.31 -0.9 236,716 + IAI47_20020 0.31 +0.7 236,716 + IAI47_20020 0.31 +0.1 236,716 + IAI47_20020 0.31 -1.0 236,716 + IAI47_20020 0.31 -1.1 236,716 + IAI47_20020 0.31 +1.2 236,717 - IAI47_20020 0.31 +0.8 236,717 - IAI47_20020 0.31 +0.7 236,717 - IAI47_20020 0.31 -1.6 236,717 - IAI47_20020 0.31 -0.7 236,717 - IAI47_20020 0.31 +0.0 236,764 + IAI47_20020 0.35 +0.3 236,764 + IAI47_20020 0.35 +0.5 236,765 - IAI47_20020 0.35 +0.4 236,778 + IAI47_20020 0.37 +2.9 236,779 - IAI47_20020 0.37 +1.8 236,779 - IAI47_20020 0.37 -1.2 236,790 + IAI47_20020 0.38 +0.0 236,790 + IAI47_20020 0.38 -0.2 236,790 + IAI47_20020 0.38 -0.2 236,790 + IAI47_20020 0.38 +0.3 236,791 - IAI47_20020 0.38 +0.0 236,791 - IAI47_20020 0.38 +0.2 236,864 + IAI47_20020 0.44 -1.5 236,872 + IAI47_20020 0.45 -0.3 236,872 + IAI47_20020 0.45 +0.3 236,884 + IAI47_20020 0.46 -0.8 236,884 + IAI47_20020 0.46 +0.3 236,888 - IAI47_20020 0.46 +0.6 237,028 + IAI47_20020 0.58 -0.4 237,028 + IAI47_20020 0.58 +0.4 237,029 - IAI47_20020 0.58 -0.5 237,031 + IAI47_20020 0.59 -1.1 237,032 - IAI47_20020 0.59 +0.7 237,032 - IAI47_20020 0.59 +1.9 237,032 - IAI47_20020 0.59 +0.0 237,032 - IAI47_20020 0.59 +1.1 237,032 - IAI47_20020 0.59 -2.5 237,066 + IAI47_20020 0.62 -0.6 237,111 + IAI47_20020 0.65 -0.5 237,112 - IAI47_20020 0.66 +2.3 237,112 - IAI47_20020 0.66 -0.5 237,326 + IAI47_20020 0.84 +1.7 237,326 + IAI47_20020 0.84 +0.7
Or see this region's nucleotide sequence