Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_19250 and IAI47_19255 are separated by 21 nucleotides IAI47_19255 and IAI47_19260 are separated by 34 nucleotides
IAI47_19250: IAI47_19250 - sugar ABC transporter ATP-binding protein, at 75,822 to 77,330
_19250
IAI47_19255: IAI47_19255 - substrate-binding domain-containing protein, at 77,352 to 78,332
_19255
IAI47_19260: IAI47_19260 - ABC transporter permease, at 78,367 to 79,392
_19260
Position (kb) 77
78
79 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 76.372 kb on + strand, within IAI47_19250 at 76.379 kb on + strand, within IAI47_19250 at 76.380 kb on - strand, within IAI47_19250 at 76.380 kb on - strand, within IAI47_19250 at 76.392 kb on + strand, within IAI47_19250 at 76.393 kb on - strand, within IAI47_19250 at 76.393 kb on - strand, within IAI47_19250 at 76.515 kb on + strand, within IAI47_19250 at 76.516 kb on - strand, within IAI47_19250 at 76.535 kb on + strand, within IAI47_19250 at 76.535 kb on + strand, within IAI47_19250 at 76.535 kb on + strand, within IAI47_19250 at 76.535 kb on + strand, within IAI47_19250 at 76.536 kb on - strand, within IAI47_19250 at 76.536 kb on - strand, within IAI47_19250 at 76.536 kb on - strand, within IAI47_19250 at 76.539 kb on + strand, within IAI47_19250 at 76.539 kb on + strand, within IAI47_19250 at 76.539 kb on + strand, within IAI47_19250 at 76.540 kb on - strand, within IAI47_19250 at 76.544 kb on + strand, within IAI47_19250 at 76.545 kb on - strand, within IAI47_19250 at 76.604 kb on - strand, within IAI47_19250 at 76.626 kb on - strand, within IAI47_19250 at 76.626 kb on - strand, within IAI47_19250 at 76.662 kb on - strand, within IAI47_19250 at 76.672 kb on + strand, within IAI47_19250 at 76.672 kb on + strand, within IAI47_19250 at 76.673 kb on - strand, within IAI47_19250 at 76.731 kb on + strand, within IAI47_19250 at 76.732 kb on - strand, within IAI47_19250 at 76.732 kb on - strand, within IAI47_19250 at 76.874 kb on + strand, within IAI47_19250 at 76.874 kb on + strand, within IAI47_19250 at 76.875 kb on - strand, within IAI47_19250 at 76.875 kb on - strand, within IAI47_19250 at 76.951 kb on + strand, within IAI47_19250 at 76.952 kb on - strand, within IAI47_19250 at 76.952 kb on - strand, within IAI47_19250 at 77.097 kb on - strand, within IAI47_19250 at 77.190 kb on + strand at 77.204 kb on + strand at 77.205 kb on - strand at 77.210 kb on + strand at 77.210 kb on + strand at 77.210 kb on + strand at 77.210 kb on + strand at 77.211 kb on - strand at 77.211 kb on - strand at 77.211 kb on - strand at 77.211 kb on - strand at 77.211 kb on - strand at 77.211 kb on - strand at 77.211 kb on - strand at 77.211 kb on - strand at 77.211 kb on - strand at 77.301 kb on + strand at 77.307 kb on + strand at 77.308 kb on - strand at 77.323 kb on + strand at 77.323 kb on + strand at 77.323 kb on + strand at 77.324 kb on - strand at 77.324 kb on - strand at 77.357 kb on - strand at 77.384 kb on + strand at 77.384 kb on + strand at 77.384 kb on + strand at 77.385 kb on - strand at 77.537 kb on + strand, within IAI47_19255 at 77.538 kb on - strand, within IAI47_19255 at 77.563 kb on + strand, within IAI47_19255 at 77.564 kb on - strand, within IAI47_19255 at 77.564 kb on - strand, within IAI47_19255 at 77.564 kb on - strand, within IAI47_19255 at 77.564 kb on - strand, within IAI47_19255 at 77.564 kb on - strand, within IAI47_19255 at 77.579 kb on + strand, within IAI47_19255 at 77.580 kb on - strand, within IAI47_19255 at 77.580 kb on - strand, within IAI47_19255 at 77.600 kb on + strand, within IAI47_19255 at 77.600 kb on + strand, within IAI47_19255 at 77.600 kb on + strand, within IAI47_19255 at 77.633 kb on + strand, within IAI47_19255 at 77.633 kb on + strand, within IAI47_19255 at 77.634 kb on - strand, within IAI47_19255 at 77.641 kb on + strand, within IAI47_19255 at 77.642 kb on - strand, within IAI47_19255 at 77.690 kb on + strand, within IAI47_19255 at 77.691 kb on - strand, within IAI47_19255 at 77.738 kb on + strand, within IAI47_19255 at 77.739 kb on - strand, within IAI47_19255 at 77.804 kb on + strand, within IAI47_19255 at 77.804 kb on + strand, within IAI47_19255 at 77.804 kb on + strand, within IAI47_19255 at 77.805 kb on - strand, within IAI47_19255 at 77.805 kb on - strand, within IAI47_19255 at 77.805 kb on - strand, within IAI47_19255 at 77.805 kb on - strand, within IAI47_19255 at 77.805 kb on - strand, within IAI47_19255 at 77.819 kb on + strand, within IAI47_19255 at 77.820 kb on - strand, within IAI47_19255 at 77.850 kb on - strand, within IAI47_19255 at 77.871 kb on + strand, within IAI47_19255 at 77.871 kb on + strand, within IAI47_19255 at 77.872 kb on - strand, within IAI47_19255 at 77.872 kb on - strand, within IAI47_19255 at 77.960 kb on + strand, within IAI47_19255 at 77.960 kb on + strand, within IAI47_19255 at 77.960 kb on + strand, within IAI47_19255 at 77.980 kb on - strand, within IAI47_19255 at 77.993 kb on + strand, within IAI47_19255 at 77.993 kb on + strand, within IAI47_19255 at 77.994 kb on - strand, within IAI47_19255 at 78.127 kb on - strand, within IAI47_19255 at 78.134 kb on + strand, within IAI47_19255 at 78.134 kb on + strand, within IAI47_19255 at 78.134 kb on + strand, within IAI47_19255 at 78.135 kb on - strand, within IAI47_19255 at 78.135 kb on - strand, within IAI47_19255 at 78.135 kb on - strand, within IAI47_19255 at 78.135 kb on - strand, within IAI47_19255 at 78.135 kb on - strand, within IAI47_19255 at 78.181 kb on + strand, within IAI47_19255 at 78.181 kb on + strand, within IAI47_19255 at 78.181 kb on + strand, within IAI47_19255 at 78.182 kb on - strand, within IAI47_19255 at 78.182 kb on - strand, within IAI47_19255 at 78.182 kb on - strand, within IAI47_19255 at 78.182 kb on - strand, within IAI47_19255 at 78.182 kb on - strand, within IAI47_19255 at 78.182 kb on - strand, within IAI47_19255 at 78.184 kb on + strand, within IAI47_19255 at 78.184 kb on + strand, within IAI47_19255 at 78.194 kb on + strand, within IAI47_19255 at 78.194 kb on + strand, within IAI47_19255 at 78.194 kb on + strand, within IAI47_19255 at 78.195 kb on - strand, within IAI47_19255 at 78.200 kb on + strand, within IAI47_19255 at 78.200 kb on + strand, within IAI47_19255 at 78.200 kb on + strand, within IAI47_19255 at 78.313 kb on - strand at 78.354 kb on - strand at 78.354 kb on - strand at 78.368 kb on + strand at 78.368 kb on + strand at 78.368 kb on + strand at 78.368 kb on + strand at 78.368 kb on + strand at 78.368 kb on + strand at 78.368 kb on + strand at 78.368 kb on + strand at 78.369 kb on - strand at 78.369 kb on - strand at 78.369 kb on - strand at 78.369 kb on - strand at 78.400 kb on + strand at 78.411 kb on + strand at 78.412 kb on - strand at 78.447 kb on + strand at 78.492 kb on - strand, within IAI47_19260 at 78.492 kb on - strand, within IAI47_19260 at 78.567 kb on + strand, within IAI47_19260 at 78.567 kb on + strand, within IAI47_19260 at 78.567 kb on + strand, within IAI47_19260 at 78.680 kb on + strand, within IAI47_19260 at 78.680 kb on + strand, within IAI47_19260 at 78.680 kb on + strand, within IAI47_19260 at 78.681 kb on - strand, within IAI47_19260 at 78.681 kb on - strand, within IAI47_19260 at 78.681 kb on - strand, within IAI47_19260 at 78.681 kb on - strand, within IAI47_19260 at 78.681 kb on - strand, within IAI47_19260 at 78.682 kb on + strand, within IAI47_19260 at 78.682 kb on + strand, within IAI47_19260 at 78.723 kb on - strand, within IAI47_19260 at 78.723 kb on - strand, within IAI47_19260 at 78.794 kb on + strand, within IAI47_19260 at 78.794 kb on + strand, within IAI47_19260 at 78.794 kb on + strand, within IAI47_19260 at 78.794 kb on + strand, within IAI47_19260 at 78.794 kb on + strand, within IAI47_19260 at 78.794 kb on + strand, within IAI47_19260 at 78.794 kb on + strand, within IAI47_19260 at 78.794 kb on + strand, within IAI47_19260 at 78.794 kb on + strand, within IAI47_19260 at 78.794 kb on + strand, within IAI47_19260 at 78.795 kb on - strand, within IAI47_19260 at 78.795 kb on - strand, within IAI47_19260 at 78.795 kb on - strand, within IAI47_19260 at 78.795 kb on - strand, within IAI47_19260 at 78.795 kb on - strand, within IAI47_19260 at 78.795 kb on - strand, within IAI47_19260 at 78.795 kb on - strand, within IAI47_19260 at 78.798 kb on + strand, within IAI47_19260 at 78.798 kb on + strand, within IAI47_19260 at 78.798 kb on + strand, within IAI47_19260 at 78.798 kb on + strand, within IAI47_19260 at 78.798 kb on + strand, within IAI47_19260 at 78.798 kb on + strand, within IAI47_19260 at 78.799 kb on - strand, within IAI47_19260 at 78.799 kb on - strand, within IAI47_19260 at 78.799 kb on - strand, within IAI47_19260 at 78.799 kb on - strand, within IAI47_19260 at 78.878 kb on - strand, within IAI47_19260 at 78.904 kb on - strand, within IAI47_19260 at 78.933 kb on + strand, within IAI47_19260 at 78.949 kb on - strand, within IAI47_19260 at 79.000 kb on - strand, within IAI47_19260 at 79.000 kb on - strand, within IAI47_19260 at 79.074 kb on + strand, within IAI47_19260 at 79.074 kb on + strand, within IAI47_19260 at 79.074 kb on + strand, within IAI47_19260 at 79.074 kb on + strand, within IAI47_19260 at 79.075 kb on - strand, within IAI47_19260 at 79.075 kb on - strand, within IAI47_19260 at 79.098 kb on + strand, within IAI47_19260 at 79.186 kb on + strand, within IAI47_19260 at 79.248 kb on + strand, within IAI47_19260 at 79.248 kb on + strand, within IAI47_19260 at 79.249 kb on - strand, within IAI47_19260 at 79.249 kb on - strand, within IAI47_19260 at 79.249 kb on - strand, within IAI47_19260 at 79.249 kb on - strand, within IAI47_19260 at 79.249 kb on - strand, within IAI47_19260
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 76,372 + IAI47_19250 0.36 -1.6 76,379 + IAI47_19250 0.37 -0.5 76,380 - IAI47_19250 0.37 -0.2 76,380 - IAI47_19250 0.37 -0.5 76,392 + IAI47_19250 0.38 -1.4 76,393 - IAI47_19250 0.38 +1.5 76,393 - IAI47_19250 0.38 -0.2 76,515 + IAI47_19250 0.46 +0.0 76,516 - IAI47_19250 0.46 +1.5 76,535 + IAI47_19250 0.47 +0.3 76,535 + IAI47_19250 0.47 -0.7 76,535 + IAI47_19250 0.47 -2.7 76,535 + IAI47_19250 0.47 +1.0 76,536 - IAI47_19250 0.47 -1.0 76,536 - IAI47_19250 0.47 -0.4 76,536 - IAI47_19250 0.47 -0.5 76,539 + IAI47_19250 0.48 +0.5 76,539 + IAI47_19250 0.48 -0.8 76,539 + IAI47_19250 0.48 +1.1 76,540 - IAI47_19250 0.48 +0.3 76,544 + IAI47_19250 0.48 +0.2 76,545 - IAI47_19250 0.48 +0.6 76,604 - IAI47_19250 0.52 -1.2 76,626 - IAI47_19250 0.53 +1.4 76,626 - IAI47_19250 0.53 -0.5 76,662 - IAI47_19250 0.56 +0.8 76,672 + IAI47_19250 0.56 +0.4 76,672 + IAI47_19250 0.56 -0.5 76,673 - IAI47_19250 0.56 +3.4 76,731 + IAI47_19250 0.60 +1.3 76,732 - IAI47_19250 0.60 +0.8 76,732 - IAI47_19250 0.60 +1.5 76,874 + IAI47_19250 0.70 +1.2 76,874 + IAI47_19250 0.70 +2.3 76,875 - IAI47_19250 0.70 -0.8 76,875 - IAI47_19250 0.70 +1.9 76,951 + IAI47_19250 0.75 +3.3 76,952 - IAI47_19250 0.75 +1.3 76,952 - IAI47_19250 0.75 +1.7 77,097 - IAI47_19250 0.84 -0.1 77,190 + -0.8 77,204 + -1.7 77,205 - -1.2 77,210 + +0.8 77,210 + +0.8 77,210 + -0.9 77,210 + -0.2 77,211 - -0.6 77,211 - +1.1 77,211 - +0.2 77,211 - +0.9 77,211 - -3.4 77,211 - -0.2 77,211 - +0.4 77,211 - +0.6 77,211 - -0.1 77,301 + +0.3 77,307 + +0.0 77,308 - -0.3 77,323 + -1.9 77,323 + +1.1 77,323 + -1.1 77,324 - +0.7 77,324 - +1.0 77,357 - -1.0 77,384 + +1.5 77,384 + -0.4 77,384 + +0.3 77,385 - +1.0 77,537 + IAI47_19255 0.19 -0.8 77,538 - IAI47_19255 0.19 +1.0 77,563 + IAI47_19255 0.22 +2.0 77,564 - IAI47_19255 0.22 +0.3 77,564 - IAI47_19255 0.22 +0.3 77,564 - IAI47_19255 0.22 -1.2 77,564 - IAI47_19255 0.22 -1.7 77,564 - IAI47_19255 0.22 +0.0 77,579 + IAI47_19255 0.23 -0.7 77,580 - IAI47_19255 0.23 -0.9 77,580 - IAI47_19255 0.23 -1.7 77,600 + IAI47_19255 0.25 +0.5 77,600 + IAI47_19255 0.25 -0.4 77,600 + IAI47_19255 0.25 +0.3 77,633 + IAI47_19255 0.29 -0.7 77,633 + IAI47_19255 0.29 -0.2 77,634 - IAI47_19255 0.29 +0.7 77,641 + IAI47_19255 0.29 +1.4 77,642 - IAI47_19255 0.30 +0.6 77,690 + IAI47_19255 0.34 +0.6 77,691 - IAI47_19255 0.35 +0.1 77,738 + IAI47_19255 0.39 -1.7 77,739 - IAI47_19255 0.39 +0.6 77,804 + IAI47_19255 0.46 -0.4 77,804 + IAI47_19255 0.46 +3.2 77,804 + IAI47_19255 0.46 +1.1 77,805 - IAI47_19255 0.46 -1.0 77,805 - IAI47_19255 0.46 -0.7 77,805 - IAI47_19255 0.46 +2.5 77,805 - IAI47_19255 0.46 -1.1 77,805 - IAI47_19255 0.46 -1.5 77,819 + IAI47_19255 0.48 +0.3 77,820 - IAI47_19255 0.48 +0.3 77,850 - IAI47_19255 0.51 -0.8 77,871 + IAI47_19255 0.53 +0.8 77,871 + IAI47_19255 0.53 -0.2 77,872 - IAI47_19255 0.53 +2.7 77,872 - IAI47_19255 0.53 +1.8 77,960 + IAI47_19255 0.62 +1.3 77,960 + IAI47_19255 0.62 +0.4 77,960 + IAI47_19255 0.62 +0.9 77,980 - IAI47_19255 0.64 -0.4 77,993 + IAI47_19255 0.65 +1.2 77,993 + IAI47_19255 0.65 +0.1 77,994 - IAI47_19255 0.65 -0.7 78,127 - IAI47_19255 0.79 +1.1 78,134 + IAI47_19255 0.80 -0.8 78,134 + IAI47_19255 0.80 +0.4 78,134 + IAI47_19255 0.80 -1.0 78,135 - IAI47_19255 0.80 -0.2 78,135 - IAI47_19255 0.80 +0.8 78,135 - IAI47_19255 0.80 -0.2 78,135 - IAI47_19255 0.80 -0.6 78,135 - IAI47_19255 0.80 -1.1 78,181 + IAI47_19255 0.85 +1.6 78,181 + IAI47_19255 0.85 -1.2 78,181 + IAI47_19255 0.85 -0.2 78,182 - IAI47_19255 0.85 -0.8 78,182 - IAI47_19255 0.85 -0.8 78,182 - IAI47_19255 0.85 -0.5 78,182 - IAI47_19255 0.85 +1.3 78,182 - IAI47_19255 0.85 -1.2 78,182 - IAI47_19255 0.85 +0.9 78,184 + IAI47_19255 0.85 +0.4 78,184 + IAI47_19255 0.85 +1.0 78,194 + IAI47_19255 0.86 -2.4 78,194 + IAI47_19255 0.86 +1.1 78,194 + IAI47_19255 0.86 -0.1 78,195 - IAI47_19255 0.86 -0.6 78,200 + IAI47_19255 0.86 +1.5 78,200 + IAI47_19255 0.86 -2.1 78,200 + IAI47_19255 0.86 -0.6 78,313 - +0.8 78,354 - +0.0 78,354 - -0.5 78,368 + +0.3 78,368 + +1.6 78,368 + +1.8 78,368 + -0.8 78,368 + +0.6 78,368 + +2.1 78,368 + -1.8 78,368 + -0.2 78,369 - +2.0 78,369 - +1.5 78,369 - -1.5 78,369 - -1.5 78,400 + -1.1 78,411 + +0.1 78,412 - +0.6 78,447 + +2.0 78,492 - IAI47_19260 0.12 +0.3 78,492 - IAI47_19260 0.12 +0.0 78,567 + IAI47_19260 0.19 +1.0 78,567 + IAI47_19260 0.19 +1.2 78,567 + IAI47_19260 0.19 -1.0 78,680 + IAI47_19260 0.31 -0.7 78,680 + IAI47_19260 0.31 +0.7 78,680 + IAI47_19260 0.31 -1.5 78,681 - IAI47_19260 0.31 -0.7 78,681 - IAI47_19260 0.31 +2.5 78,681 - IAI47_19260 0.31 -2.0 78,681 - IAI47_19260 0.31 +1.0 78,681 - IAI47_19260 0.31 +1.6 78,682 + IAI47_19260 0.31 +0.5 78,682 + IAI47_19260 0.31 -0.7 78,723 - IAI47_19260 0.35 -0.2 78,723 - IAI47_19260 0.35 +1.6 78,794 + IAI47_19260 0.42 +0.8 78,794 + IAI47_19260 0.42 -1.2 78,794 + IAI47_19260 0.42 +0.4 78,794 + IAI47_19260 0.42 +0.9 78,794 + IAI47_19260 0.42 +1.3 78,794 + IAI47_19260 0.42 +0.1 78,794 + IAI47_19260 0.42 -0.3 78,794 + IAI47_19260 0.42 +1.2 78,794 + IAI47_19260 0.42 -0.7 78,794 + IAI47_19260 0.42 +1.2 78,795 - IAI47_19260 0.42 +0.9 78,795 - IAI47_19260 0.42 +0.4 78,795 - IAI47_19260 0.42 -2.1 78,795 - IAI47_19260 0.42 -0.6 78,795 - IAI47_19260 0.42 +2.2 78,795 - IAI47_19260 0.42 +0.2 78,795 - IAI47_19260 0.42 +0.4 78,798 + IAI47_19260 0.42 -1.8 78,798 + IAI47_19260 0.42 -1.0 78,798 + IAI47_19260 0.42 +0.8 78,798 + IAI47_19260 0.42 +2.0 78,798 + IAI47_19260 0.42 -0.5 78,798 + IAI47_19260 0.42 +1.1 78,799 - IAI47_19260 0.42 +1.0 78,799 - IAI47_19260 0.42 +0.1 78,799 - IAI47_19260 0.42 +0.2 78,799 - IAI47_19260 0.42 -0.2 78,878 - IAI47_19260 0.50 -0.1 78,904 - IAI47_19260 0.52 +0.0 78,933 + IAI47_19260 0.55 +0.6 78,949 - IAI47_19260 0.57 +0.4 79,000 - IAI47_19260 0.62 +0.3 79,000 - IAI47_19260 0.62 +0.5 79,074 + IAI47_19260 0.69 -1.3 79,074 + IAI47_19260 0.69 -1.2 79,074 + IAI47_19260 0.69 +0.3 79,074 + IAI47_19260 0.69 +2.3 79,075 - IAI47_19260 0.69 -1.5 79,075 - IAI47_19260 0.69 +1.8 79,098 + IAI47_19260 0.71 -0.2 79,186 + IAI47_19260 0.80 +1.9 79,248 + IAI47_19260 0.86 +0.8 79,248 + IAI47_19260 0.86 -0.1 79,249 - IAI47_19260 0.86 -1.4 79,249 - IAI47_19260 0.86 -1.2 79,249 - IAI47_19260 0.86 +2.1 79,249 - IAI47_19260 0.86 -0.2 79,249 - IAI47_19260 0.86 -1.3
Or see this region's nucleotide sequence