Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_19240 and IAI47_19245 are separated by 14 nucleotides IAI47_19245 and IAI47_19250 are separated by 272 nucleotides IAI47_19250 and IAI47_19255 are separated by 21 nucleotides
IAI47_19240: IAI47_19240 - SDR family oxidoreductase, at 74,222 to 74,974
_19240
IAI47_19245: IAI47_19245 - SIS domain-containing protein, at 74,989 to 75,549
_19245
IAI47_19250: IAI47_19250 - sugar ABC transporter ATP-binding protein, at 75,822 to 77,330
_19250
IAI47_19255: IAI47_19255 - substrate-binding domain-containing protein, at 77,352 to 78,332
_19255
Position (kb) 75
76
77
78 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 74.911 kb on + strand at 74.911 kb on + strand at 74.911 kb on + strand at 74.912 kb on - strand at 74.924 kb on + strand at 74.925 kb on - strand at 74.947 kb on + strand at 74.948 kb on - strand at 74.983 kb on + strand at 75.134 kb on + strand, within IAI47_19245 at 75.264 kb on - strand, within IAI47_19245 at 75.264 kb on - strand, within IAI47_19245 at 75.270 kb on + strand at 75.270 kb on + strand, within IAI47_19245 at 75.271 kb on - strand, within IAI47_19245 at 75.271 kb on - strand, within IAI47_19245 at 75.271 kb on - strand, within IAI47_19245 at 75.271 kb on - strand, within IAI47_19245 at 75.366 kb on - strand, within IAI47_19245 at 75.466 kb on + strand, within IAI47_19245 at 75.466 kb on + strand, within IAI47_19245 at 75.467 kb on - strand, within IAI47_19245 at 75.467 kb on - strand, within IAI47_19245 at 75.467 kb on - strand, within IAI47_19245 at 75.467 kb on - strand, within IAI47_19245 at 75.479 kb on + strand, within IAI47_19245 at 75.479 kb on + strand, within IAI47_19245 at 75.479 kb on + strand, within IAI47_19245 at 75.480 kb on - strand, within IAI47_19245 at 75.480 kb on - strand, within IAI47_19245 at 75.548 kb on - strand at 75.550 kb on + strand at 75.550 kb on + strand at 75.586 kb on + strand at 75.586 kb on + strand at 75.608 kb on + strand at 75.608 kb on + strand at 75.608 kb on + strand at 75.608 kb on + strand at 75.608 kb on + strand at 75.609 kb on - strand at 75.609 kb on - strand at 75.609 kb on - strand at 75.622 kb on + strand at 75.623 kb on - strand at 75.623 kb on - strand at 75.638 kb on + strand at 75.639 kb on - strand at 75.665 kb on + strand at 75.666 kb on - strand at 75.666 kb on - strand at 75.666 kb on - strand at 75.671 kb on + strand at 75.672 kb on - strand at 75.672 kb on - strand at 75.672 kb on - strand at 75.689 kb on + strand at 75.708 kb on + strand at 75.708 kb on + strand at 75.709 kb on - strand at 75.709 kb on - strand at 75.747 kb on + strand at 75.748 kb on - strand at 75.767 kb on + strand at 75.767 kb on + strand at 75.767 kb on + strand at 75.768 kb on - strand at 75.773 kb on + strand at 75.773 kb on + strand at 75.774 kb on - strand at 75.774 kb on - strand at 75.810 kb on + strand at 75.823 kb on + strand at 75.824 kb on - strand at 75.876 kb on - strand at 75.930 kb on - strand at 75.935 kb on + strand at 76.013 kb on + strand, within IAI47_19250 at 76.013 kb on + strand, within IAI47_19250 at 76.014 kb on - strand, within IAI47_19250 at 76.014 kb on - strand, within IAI47_19250 at 76.025 kb on + strand, within IAI47_19250 at 76.026 kb on - strand, within IAI47_19250 at 76.075 kb on - strand, within IAI47_19250 at 76.083 kb on - strand, within IAI47_19250 at 76.083 kb on - strand, within IAI47_19250 at 76.146 kb on - strand at 76.146 kb on - strand, within IAI47_19250 at 76.167 kb on + strand, within IAI47_19250 at 76.167 kb on + strand, within IAI47_19250 at 76.167 kb on + strand, within IAI47_19250 at 76.167 kb on + strand, within IAI47_19250 at 76.167 kb on + strand, within IAI47_19250 at 76.168 kb on - strand, within IAI47_19250 at 76.168 kb on - strand, within IAI47_19250 at 76.168 kb on - strand, within IAI47_19250 at 76.168 kb on - strand, within IAI47_19250 at 76.173 kb on - strand, within IAI47_19250 at 76.276 kb on + strand, within IAI47_19250 at 76.276 kb on + strand, within IAI47_19250 at 76.276 kb on + strand, within IAI47_19250 at 76.276 kb on + strand, within IAI47_19250 at 76.277 kb on - strand, within IAI47_19250 at 76.277 kb on - strand, within IAI47_19250 at 76.295 kb on + strand, within IAI47_19250 at 76.295 kb on + strand, within IAI47_19250 at 76.295 kb on + strand, within IAI47_19250 at 76.327 kb on + strand, within IAI47_19250 at 76.327 kb on + strand, within IAI47_19250 at 76.327 kb on + strand, within IAI47_19250 at 76.372 kb on + strand, within IAI47_19250 at 76.379 kb on + strand, within IAI47_19250 at 76.380 kb on - strand, within IAI47_19250 at 76.380 kb on - strand, within IAI47_19250 at 76.392 kb on + strand, within IAI47_19250 at 76.393 kb on - strand, within IAI47_19250 at 76.393 kb on - strand, within IAI47_19250 at 76.515 kb on + strand, within IAI47_19250 at 76.516 kb on - strand, within IAI47_19250 at 76.535 kb on + strand, within IAI47_19250 at 76.535 kb on + strand, within IAI47_19250 at 76.535 kb on + strand, within IAI47_19250 at 76.535 kb on + strand, within IAI47_19250 at 76.536 kb on - strand, within IAI47_19250 at 76.536 kb on - strand, within IAI47_19250 at 76.536 kb on - strand, within IAI47_19250 at 76.539 kb on + strand, within IAI47_19250 at 76.539 kb on + strand, within IAI47_19250 at 76.539 kb on + strand, within IAI47_19250 at 76.540 kb on - strand, within IAI47_19250 at 76.544 kb on + strand, within IAI47_19250 at 76.545 kb on - strand, within IAI47_19250 at 76.604 kb on - strand, within IAI47_19250 at 76.626 kb on - strand, within IAI47_19250 at 76.626 kb on - strand, within IAI47_19250 at 76.662 kb on - strand, within IAI47_19250 at 76.672 kb on + strand, within IAI47_19250 at 76.672 kb on + strand, within IAI47_19250 at 76.673 kb on - strand, within IAI47_19250 at 76.731 kb on + strand, within IAI47_19250 at 76.732 kb on - strand, within IAI47_19250 at 76.732 kb on - strand, within IAI47_19250 at 76.874 kb on + strand, within IAI47_19250 at 76.874 kb on + strand, within IAI47_19250 at 76.875 kb on - strand, within IAI47_19250 at 76.875 kb on - strand, within IAI47_19250 at 76.951 kb on + strand, within IAI47_19250 at 76.952 kb on - strand, within IAI47_19250 at 76.952 kb on - strand, within IAI47_19250 at 77.097 kb on - strand, within IAI47_19250 at 77.190 kb on + strand at 77.204 kb on + strand at 77.205 kb on - strand at 77.210 kb on + strand at 77.210 kb on + strand at 77.210 kb on + strand at 77.210 kb on + strand at 77.211 kb on - strand at 77.211 kb on - strand at 77.211 kb on - strand at 77.211 kb on - strand at 77.211 kb on - strand at 77.211 kb on - strand at 77.211 kb on - strand at 77.211 kb on - strand at 77.211 kb on - strand at 77.301 kb on + strand at 77.307 kb on + strand at 77.308 kb on - strand at 77.323 kb on + strand at 77.323 kb on + strand at 77.323 kb on + strand at 77.324 kb on - strand at 77.324 kb on - strand at 77.357 kb on - strand at 77.384 kb on + strand at 77.384 kb on + strand at 77.384 kb on + strand at 77.385 kb on - strand at 77.537 kb on + strand, within IAI47_19255 at 77.538 kb on - strand, within IAI47_19255 at 77.563 kb on + strand, within IAI47_19255 at 77.564 kb on - strand, within IAI47_19255 at 77.564 kb on - strand, within IAI47_19255 at 77.564 kb on - strand, within IAI47_19255 at 77.564 kb on - strand, within IAI47_19255 at 77.564 kb on - strand, within IAI47_19255 at 77.579 kb on + strand, within IAI47_19255 at 77.580 kb on - strand, within IAI47_19255 at 77.580 kb on - strand, within IAI47_19255 at 77.600 kb on + strand, within IAI47_19255 at 77.600 kb on + strand, within IAI47_19255 at 77.600 kb on + strand, within IAI47_19255 at 77.633 kb on + strand, within IAI47_19255 at 77.633 kb on + strand, within IAI47_19255 at 77.634 kb on - strand, within IAI47_19255 at 77.641 kb on + strand, within IAI47_19255 at 77.642 kb on - strand, within IAI47_19255 at 77.690 kb on + strand, within IAI47_19255 at 77.691 kb on - strand, within IAI47_19255 at 77.738 kb on + strand, within IAI47_19255 at 77.739 kb on - strand, within IAI47_19255 at 77.804 kb on + strand, within IAI47_19255 at 77.804 kb on + strand, within IAI47_19255 at 77.804 kb on + strand, within IAI47_19255 at 77.805 kb on - strand, within IAI47_19255 at 77.805 kb on - strand, within IAI47_19255 at 77.805 kb on - strand, within IAI47_19255 at 77.805 kb on - strand, within IAI47_19255 at 77.805 kb on - strand, within IAI47_19255 at 77.819 kb on + strand, within IAI47_19255 at 77.820 kb on - strand, within IAI47_19255 at 77.850 kb on - strand, within IAI47_19255 at 77.871 kb on + strand, within IAI47_19255 at 77.871 kb on + strand, within IAI47_19255 at 77.872 kb on - strand, within IAI47_19255 at 77.872 kb on - strand, within IAI47_19255 at 77.960 kb on + strand, within IAI47_19255 at 77.960 kb on + strand, within IAI47_19255 at 77.960 kb on + strand, within IAI47_19255 at 77.980 kb on - strand, within IAI47_19255 at 77.993 kb on + strand, within IAI47_19255 at 77.993 kb on + strand, within IAI47_19255 at 77.994 kb on - strand, within IAI47_19255 at 78.127 kb on - strand, within IAI47_19255 at 78.134 kb on + strand, within IAI47_19255 at 78.134 kb on + strand, within IAI47_19255 at 78.134 kb on + strand, within IAI47_19255 at 78.135 kb on - strand, within IAI47_19255 at 78.135 kb on - strand, within IAI47_19255 at 78.135 kb on - strand, within IAI47_19255 at 78.135 kb on - strand, within IAI47_19255 at 78.135 kb on - strand, within IAI47_19255 at 78.181 kb on + strand, within IAI47_19255 at 78.181 kb on + strand, within IAI47_19255 at 78.181 kb on + strand, within IAI47_19255 at 78.182 kb on - strand, within IAI47_19255 at 78.182 kb on - strand, within IAI47_19255 at 78.182 kb on - strand, within IAI47_19255 at 78.182 kb on - strand, within IAI47_19255 at 78.182 kb on - strand, within IAI47_19255 at 78.182 kb on - strand, within IAI47_19255 at 78.184 kb on + strand, within IAI47_19255 at 78.184 kb on + strand, within IAI47_19255 at 78.194 kb on + strand, within IAI47_19255 at 78.194 kb on + strand, within IAI47_19255 at 78.194 kb on + strand, within IAI47_19255 at 78.195 kb on - strand, within IAI47_19255 at 78.200 kb on + strand, within IAI47_19255 at 78.200 kb on + strand, within IAI47_19255 at 78.200 kb on + strand, within IAI47_19255 at 78.313 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 74,911 + +1.1 74,911 + -1.4 74,911 + +2.8 74,912 - -0.8 74,924 + -0.0 74,925 - -1.1 74,947 + +1.6 74,948 - +1.1 74,983 + +0.5 75,134 + IAI47_19245 0.26 +0.8 75,264 - IAI47_19245 0.49 +2.8 75,264 - IAI47_19245 0.49 +1.5 75,270 + -1.9 75,270 + IAI47_19245 0.50 -0.9 75,271 - IAI47_19245 0.50 -0.1 75,271 - IAI47_19245 0.50 +1.9 75,271 - IAI47_19245 0.50 +0.1 75,271 - IAI47_19245 0.50 +4.9 75,366 - IAI47_19245 0.67 +0.6 75,466 + IAI47_19245 0.85 -0.8 75,466 + IAI47_19245 0.85 -0.7 75,467 - IAI47_19245 0.85 +3.2 75,467 - IAI47_19245 0.85 -1.4 75,467 - IAI47_19245 0.85 +0.7 75,467 - IAI47_19245 0.85 -0.3 75,479 + IAI47_19245 0.87 +0.3 75,479 + IAI47_19245 0.87 -0.2 75,479 + IAI47_19245 0.87 +0.6 75,480 - IAI47_19245 0.88 -1.0 75,480 - IAI47_19245 0.88 +0.4 75,548 - -0.6 75,550 + +0.6 75,550 + -0.6 75,586 + -2.4 75,586 + +1.0 75,608 + +0.8 75,608 + +0.5 75,608 + +0.5 75,608 + -0.1 75,608 + +0.2 75,609 - +1.3 75,609 - -2.0 75,609 - -0.5 75,622 + -0.9 75,623 - -1.6 75,623 - -0.9 75,638 + -0.4 75,639 - +1.3 75,665 + +1.1 75,666 - +0.8 75,666 - -0.9 75,666 - +0.0 75,671 + +1.3 75,672 - +0.8 75,672 - +0.5 75,672 - +0.1 75,689 + +0.3 75,708 + +0.4 75,708 + +0.6 75,709 - +0.7 75,709 - -0.7 75,747 + -1.1 75,748 - +0.6 75,767 + +1.9 75,767 + +0.8 75,767 + +0.5 75,768 - +2.6 75,773 + -1.7 75,773 + -0.9 75,774 - -1.5 75,774 - +1.0 75,810 + -0.8 75,823 + +0.1 75,824 - -0.2 75,876 - -0.5 75,930 - -0.5 75,935 + -0.8 76,013 + IAI47_19250 0.13 +0.8 76,013 + IAI47_19250 0.13 +0.0 76,014 - IAI47_19250 0.13 +1.0 76,014 - IAI47_19250 0.13 +0.9 76,025 + IAI47_19250 0.13 +1.5 76,026 - IAI47_19250 0.14 +0.6 76,075 - IAI47_19250 0.17 -0.4 76,083 - IAI47_19250 0.17 +0.9 76,083 - IAI47_19250 0.17 -0.1 76,146 - -0.7 76,146 - IAI47_19250 0.21 +1.1 76,167 + IAI47_19250 0.23 -0.6 76,167 + IAI47_19250 0.23 +0.8 76,167 + IAI47_19250 0.23 -1.1 76,167 + IAI47_19250 0.23 -0.7 76,167 + IAI47_19250 0.23 -0.3 76,168 - IAI47_19250 0.23 +0.5 76,168 - IAI47_19250 0.23 +1.3 76,168 - IAI47_19250 0.23 +0.3 76,168 - IAI47_19250 0.23 -0.2 76,173 - IAI47_19250 0.23 +0.6 76,276 + IAI47_19250 0.30 -0.3 76,276 + IAI47_19250 0.30 +0.3 76,276 + IAI47_19250 0.30 +1.5 76,276 + IAI47_19250 0.30 -0.8 76,277 - IAI47_19250 0.30 +0.5 76,277 - IAI47_19250 0.30 -0.7 76,295 + IAI47_19250 0.31 +1.2 76,295 + IAI47_19250 0.31 +1.3 76,295 + IAI47_19250 0.31 +0.7 76,327 + IAI47_19250 0.33 +0.3 76,327 + IAI47_19250 0.33 +2.6 76,327 + IAI47_19250 0.33 +0.3 76,372 + IAI47_19250 0.36 -1.6 76,379 + IAI47_19250 0.37 -0.5 76,380 - IAI47_19250 0.37 -0.2 76,380 - IAI47_19250 0.37 -0.5 76,392 + IAI47_19250 0.38 -1.4 76,393 - IAI47_19250 0.38 +1.5 76,393 - IAI47_19250 0.38 -0.2 76,515 + IAI47_19250 0.46 +0.0 76,516 - IAI47_19250 0.46 +1.5 76,535 + IAI47_19250 0.47 +0.3 76,535 + IAI47_19250 0.47 -0.7 76,535 + IAI47_19250 0.47 -2.7 76,535 + IAI47_19250 0.47 +1.0 76,536 - IAI47_19250 0.47 -1.0 76,536 - IAI47_19250 0.47 -0.4 76,536 - IAI47_19250 0.47 -0.5 76,539 + IAI47_19250 0.48 +0.5 76,539 + IAI47_19250 0.48 -0.8 76,539 + IAI47_19250 0.48 +1.1 76,540 - IAI47_19250 0.48 +0.3 76,544 + IAI47_19250 0.48 +0.2 76,545 - IAI47_19250 0.48 +0.6 76,604 - IAI47_19250 0.52 -1.2 76,626 - IAI47_19250 0.53 +1.4 76,626 - IAI47_19250 0.53 -0.5 76,662 - IAI47_19250 0.56 +0.8 76,672 + IAI47_19250 0.56 +0.4 76,672 + IAI47_19250 0.56 -0.5 76,673 - IAI47_19250 0.56 +3.4 76,731 + IAI47_19250 0.60 +1.3 76,732 - IAI47_19250 0.60 +0.8 76,732 - IAI47_19250 0.60 +1.5 76,874 + IAI47_19250 0.70 +1.2 76,874 + IAI47_19250 0.70 +2.3 76,875 - IAI47_19250 0.70 -0.8 76,875 - IAI47_19250 0.70 +1.9 76,951 + IAI47_19250 0.75 +3.3 76,952 - IAI47_19250 0.75 +1.3 76,952 - IAI47_19250 0.75 +1.7 77,097 - IAI47_19250 0.84 -0.1 77,190 + -0.8 77,204 + -1.7 77,205 - -1.2 77,210 + +0.8 77,210 + +0.8 77,210 + -0.9 77,210 + -0.2 77,211 - -0.6 77,211 - +1.1 77,211 - +0.2 77,211 - +0.9 77,211 - -3.4 77,211 - -0.2 77,211 - +0.4 77,211 - +0.6 77,211 - -0.1 77,301 + +0.3 77,307 + +0.0 77,308 - -0.3 77,323 + -1.9 77,323 + +1.1 77,323 + -1.1 77,324 - +0.7 77,324 - +1.0 77,357 - -1.0 77,384 + +1.5 77,384 + -0.4 77,384 + +0.3 77,385 - +1.0 77,537 + IAI47_19255 0.19 -0.8 77,538 - IAI47_19255 0.19 +1.0 77,563 + IAI47_19255 0.22 +2.0 77,564 - IAI47_19255 0.22 +0.3 77,564 - IAI47_19255 0.22 +0.3 77,564 - IAI47_19255 0.22 -1.2 77,564 - IAI47_19255 0.22 -1.7 77,564 - IAI47_19255 0.22 +0.0 77,579 + IAI47_19255 0.23 -0.7 77,580 - IAI47_19255 0.23 -0.9 77,580 - IAI47_19255 0.23 -1.7 77,600 + IAI47_19255 0.25 +0.5 77,600 + IAI47_19255 0.25 -0.4 77,600 + IAI47_19255 0.25 +0.3 77,633 + IAI47_19255 0.29 -0.7 77,633 + IAI47_19255 0.29 -0.2 77,634 - IAI47_19255 0.29 +0.7 77,641 + IAI47_19255 0.29 +1.4 77,642 - IAI47_19255 0.30 +0.6 77,690 + IAI47_19255 0.34 +0.6 77,691 - IAI47_19255 0.35 +0.1 77,738 + IAI47_19255 0.39 -1.7 77,739 - IAI47_19255 0.39 +0.6 77,804 + IAI47_19255 0.46 -0.4 77,804 + IAI47_19255 0.46 +3.2 77,804 + IAI47_19255 0.46 +1.1 77,805 - IAI47_19255 0.46 -1.0 77,805 - IAI47_19255 0.46 -0.7 77,805 - IAI47_19255 0.46 +2.5 77,805 - IAI47_19255 0.46 -1.1 77,805 - IAI47_19255 0.46 -1.5 77,819 + IAI47_19255 0.48 +0.3 77,820 - IAI47_19255 0.48 +0.3 77,850 - IAI47_19255 0.51 -0.8 77,871 + IAI47_19255 0.53 +0.8 77,871 + IAI47_19255 0.53 -0.2 77,872 - IAI47_19255 0.53 +2.7 77,872 - IAI47_19255 0.53 +1.8 77,960 + IAI47_19255 0.62 +1.3 77,960 + IAI47_19255 0.62 +0.4 77,960 + IAI47_19255 0.62 +0.9 77,980 - IAI47_19255 0.64 -0.4 77,993 + IAI47_19255 0.65 +1.2 77,993 + IAI47_19255 0.65 +0.1 77,994 - IAI47_19255 0.65 -0.7 78,127 - IAI47_19255 0.79 +1.1 78,134 + IAI47_19255 0.80 -0.8 78,134 + IAI47_19255 0.80 +0.4 78,134 + IAI47_19255 0.80 -1.0 78,135 - IAI47_19255 0.80 -0.2 78,135 - IAI47_19255 0.80 +0.8 78,135 - IAI47_19255 0.80 -0.2 78,135 - IAI47_19255 0.80 -0.6 78,135 - IAI47_19255 0.80 -1.1 78,181 + IAI47_19255 0.85 +1.6 78,181 + IAI47_19255 0.85 -1.2 78,181 + IAI47_19255 0.85 -0.2 78,182 - IAI47_19255 0.85 -0.8 78,182 - IAI47_19255 0.85 -0.8 78,182 - IAI47_19255 0.85 -0.5 78,182 - IAI47_19255 0.85 +1.3 78,182 - IAI47_19255 0.85 -1.2 78,182 - IAI47_19255 0.85 +0.9 78,184 + IAI47_19255 0.85 +0.4 78,184 + IAI47_19255 0.85 +1.0 78,194 + IAI47_19255 0.86 -2.4 78,194 + IAI47_19255 0.86 +1.1 78,194 + IAI47_19255 0.86 -0.1 78,195 - IAI47_19255 0.86 -0.6 78,200 + IAI47_19255 0.86 +1.5 78,200 + IAI47_19255 0.86 -2.1 78,200 + IAI47_19255 0.86 -0.6 78,313 - +0.8
Or see this region's nucleotide sequence