Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_19235 and IAI47_19240 are separated by 111 nucleotides IAI47_19240 and IAI47_19245 are separated by 14 nucleotides IAI47_19245 and IAI47_19250 are separated by 272 nucleotides
IAI47_19235: IAI47_19235 - ribulose-phosphate 3-epimerase, at 73,466 to 74,110
_19235
IAI47_19240: IAI47_19240 - SDR family oxidoreductase, at 74,222 to 74,974
_19240
IAI47_19245: IAI47_19245 - SIS domain-containing protein, at 74,989 to 75,549
_19245
IAI47_19250: IAI47_19250 - sugar ABC transporter ATP-binding protein, at 75,822 to 77,330
_19250
Position (kb) 74
75
76 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 73.990 kb on + strand, within IAI47_19235 at 73.990 kb on + strand, within IAI47_19235 at 73.990 kb on + strand, within IAI47_19235 at 73.991 kb on - strand, within IAI47_19235 at 73.991 kb on - strand, within IAI47_19235 at 74.008 kb on + strand, within IAI47_19235 at 74.008 kb on + strand, within IAI47_19235 at 74.008 kb on + strand, within IAI47_19235 at 74.014 kb on + strand, within IAI47_19235 at 74.034 kb on + strand, within IAI47_19235 at 74.034 kb on + strand, within IAI47_19235 at 74.034 kb on + strand, within IAI47_19235 at 74.034 kb on + strand, within IAI47_19235 at 74.035 kb on - strand, within IAI47_19235 at 74.055 kb on + strand at 74.056 kb on - strand at 74.142 kb on + strand at 74.142 kb on + strand at 74.142 kb on + strand at 74.142 kb on + strand at 74.143 kb on - strand at 74.145 kb on + strand at 74.145 kb on + strand at 74.145 kb on + strand at 74.146 kb on - strand at 74.146 kb on - strand at 74.146 kb on - strand at 74.161 kb on + strand at 74.162 kb on - strand at 74.162 kb on - strand at 74.162 kb on - strand at 74.162 kb on - strand at 74.162 kb on - strand at 74.164 kb on + strand at 74.228 kb on - strand at 74.228 kb on - strand at 74.250 kb on + strand at 74.251 kb on - strand at 74.333 kb on + strand, within IAI47_19240 at 74.333 kb on + strand, within IAI47_19240 at 74.334 kb on - strand, within IAI47_19240 at 74.334 kb on - strand, within IAI47_19240 at 74.412 kb on + strand, within IAI47_19240 at 74.413 kb on - strand, within IAI47_19240 at 74.433 kb on + strand, within IAI47_19240 at 74.433 kb on + strand, within IAI47_19240 at 74.433 kb on + strand, within IAI47_19240 at 74.434 kb on - strand, within IAI47_19240 at 74.434 kb on - strand, within IAI47_19240 at 74.434 kb on - strand, within IAI47_19240 at 74.443 kb on + strand, within IAI47_19240 at 74.444 kb on - strand, within IAI47_19240 at 74.446 kb on + strand, within IAI47_19240 at 74.447 kb on - strand, within IAI47_19240 at 74.470 kb on + strand, within IAI47_19240 at 74.512 kb on + strand, within IAI47_19240 at 74.524 kb on + strand, within IAI47_19240 at 74.524 kb on + strand, within IAI47_19240 at 74.527 kb on + strand, within IAI47_19240 at 74.528 kb on - strand, within IAI47_19240 at 74.528 kb on - strand, within IAI47_19240 at 74.545 kb on + strand, within IAI47_19240 at 74.574 kb on + strand, within IAI47_19240 at 74.578 kb on + strand, within IAI47_19240 at 74.578 kb on + strand, within IAI47_19240 at 74.597 kb on + strand, within IAI47_19240 at 74.597 kb on + strand, within IAI47_19240 at 74.597 kb on + strand, within IAI47_19240 at 74.633 kb on + strand, within IAI47_19240 at 74.634 kb on - strand, within IAI47_19240 at 74.634 kb on - strand, within IAI47_19240 at 74.635 kb on + strand, within IAI47_19240 at 74.635 kb on + strand, within IAI47_19240 at 74.635 kb on + strand, within IAI47_19240 at 74.635 kb on + strand, within IAI47_19240 at 74.635 kb on + strand, within IAI47_19240 at 74.635 kb on + strand, within IAI47_19240 at 74.636 kb on - strand, within IAI47_19240 at 74.682 kb on - strand, within IAI47_19240 at 74.692 kb on + strand, within IAI47_19240 at 74.692 kb on + strand, within IAI47_19240 at 74.693 kb on - strand, within IAI47_19240 at 74.715 kb on + strand, within IAI47_19240 at 74.716 kb on - strand, within IAI47_19240 at 74.806 kb on + strand, within IAI47_19240 at 74.806 kb on + strand, within IAI47_19240 at 74.806 kb on + strand, within IAI47_19240 at 74.806 kb on + strand, within IAI47_19240 at 74.807 kb on - strand, within IAI47_19240 at 74.810 kb on + strand, within IAI47_19240 at 74.811 kb on - strand, within IAI47_19240 at 74.815 kb on + strand, within IAI47_19240 at 74.815 kb on + strand, within IAI47_19240 at 74.815 kb on + strand, within IAI47_19240 at 74.816 kb on - strand, within IAI47_19240 at 74.911 kb on + strand at 74.911 kb on + strand at 74.911 kb on + strand at 74.912 kb on - strand at 74.924 kb on + strand at 74.925 kb on - strand at 74.947 kb on + strand at 74.948 kb on - strand at 74.983 kb on + strand at 75.134 kb on + strand, within IAI47_19245 at 75.264 kb on - strand, within IAI47_19245 at 75.264 kb on - strand, within IAI47_19245 at 75.270 kb on + strand at 75.270 kb on + strand, within IAI47_19245 at 75.271 kb on - strand, within IAI47_19245 at 75.271 kb on - strand, within IAI47_19245 at 75.271 kb on - strand, within IAI47_19245 at 75.271 kb on - strand, within IAI47_19245 at 75.366 kb on - strand, within IAI47_19245 at 75.466 kb on + strand, within IAI47_19245 at 75.466 kb on + strand, within IAI47_19245 at 75.467 kb on - strand, within IAI47_19245 at 75.467 kb on - strand, within IAI47_19245 at 75.467 kb on - strand, within IAI47_19245 at 75.467 kb on - strand, within IAI47_19245 at 75.479 kb on + strand, within IAI47_19245 at 75.479 kb on + strand, within IAI47_19245 at 75.479 kb on + strand, within IAI47_19245 at 75.480 kb on - strand, within IAI47_19245 at 75.480 kb on - strand, within IAI47_19245 at 75.548 kb on - strand at 75.550 kb on + strand at 75.550 kb on + strand at 75.586 kb on + strand at 75.586 kb on + strand at 75.608 kb on + strand at 75.608 kb on + strand at 75.608 kb on + strand at 75.608 kb on + strand at 75.608 kb on + strand at 75.609 kb on - strand at 75.609 kb on - strand at 75.609 kb on - strand at 75.622 kb on + strand at 75.623 kb on - strand at 75.623 kb on - strand at 75.638 kb on + strand at 75.639 kb on - strand at 75.665 kb on + strand at 75.666 kb on - strand at 75.666 kb on - strand at 75.666 kb on - strand at 75.671 kb on + strand at 75.672 kb on - strand at 75.672 kb on - strand at 75.672 kb on - strand at 75.689 kb on + strand at 75.708 kb on + strand at 75.708 kb on + strand at 75.709 kb on - strand at 75.709 kb on - strand at 75.747 kb on + strand at 75.748 kb on - strand at 75.767 kb on + strand at 75.767 kb on + strand at 75.767 kb on + strand at 75.768 kb on - strand at 75.773 kb on + strand at 75.773 kb on + strand at 75.774 kb on - strand at 75.774 kb on - strand at 75.810 kb on + strand at 75.823 kb on + strand at 75.824 kb on - strand at 75.876 kb on - strand at 75.930 kb on - strand at 75.935 kb on + strand at 76.013 kb on + strand, within IAI47_19250 at 76.013 kb on + strand, within IAI47_19250 at 76.014 kb on - strand, within IAI47_19250 at 76.014 kb on - strand, within IAI47_19250 at 76.025 kb on + strand, within IAI47_19250 at 76.026 kb on - strand, within IAI47_19250 at 76.075 kb on - strand, within IAI47_19250 at 76.083 kb on - strand, within IAI47_19250 at 76.083 kb on - strand, within IAI47_19250 at 76.146 kb on - strand at 76.146 kb on - strand, within IAI47_19250 at 76.167 kb on + strand, within IAI47_19250 at 76.167 kb on + strand, within IAI47_19250 at 76.167 kb on + strand, within IAI47_19250 at 76.167 kb on + strand, within IAI47_19250 at 76.167 kb on + strand, within IAI47_19250 at 76.168 kb on - strand, within IAI47_19250 at 76.168 kb on - strand, within IAI47_19250 at 76.168 kb on - strand, within IAI47_19250 at 76.168 kb on - strand, within IAI47_19250 at 76.173 kb on - strand, within IAI47_19250 at 76.276 kb on + strand, within IAI47_19250 at 76.276 kb on + strand, within IAI47_19250 at 76.276 kb on + strand, within IAI47_19250 at 76.276 kb on + strand, within IAI47_19250 at 76.277 kb on - strand, within IAI47_19250 at 76.277 kb on - strand, within IAI47_19250 at 76.295 kb on + strand, within IAI47_19250 at 76.295 kb on + strand, within IAI47_19250 at 76.295 kb on + strand, within IAI47_19250 at 76.327 kb on + strand, within IAI47_19250 at 76.327 kb on + strand, within IAI47_19250 at 76.327 kb on + strand, within IAI47_19250 at 76.372 kb on + strand, within IAI47_19250 at 76.379 kb on + strand, within IAI47_19250 at 76.380 kb on - strand, within IAI47_19250 at 76.380 kb on - strand, within IAI47_19250 at 76.392 kb on + strand, within IAI47_19250 at 76.393 kb on - strand, within IAI47_19250 at 76.393 kb on - strand, within IAI47_19250 at 76.515 kb on + strand, within IAI47_19250 at 76.516 kb on - strand, within IAI47_19250 at 76.535 kb on + strand, within IAI47_19250 at 76.535 kb on + strand, within IAI47_19250 at 76.535 kb on + strand, within IAI47_19250 at 76.535 kb on + strand, within IAI47_19250 at 76.536 kb on - strand, within IAI47_19250 at 76.536 kb on - strand, within IAI47_19250 at 76.536 kb on - strand, within IAI47_19250 at 76.539 kb on + strand, within IAI47_19250 at 76.539 kb on + strand, within IAI47_19250 at 76.539 kb on + strand, within IAI47_19250 at 76.540 kb on - strand, within IAI47_19250 at 76.544 kb on + strand, within IAI47_19250 at 76.545 kb on - strand, within IAI47_19250
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 73,990 + IAI47_19235 0.81 +0.2 73,990 + IAI47_19235 0.81 -0.8 73,990 + IAI47_19235 0.81 -0.5 73,991 - IAI47_19235 0.81 -0.9 73,991 - IAI47_19235 0.81 +0.8 74,008 + IAI47_19235 0.84 -0.7 74,008 + IAI47_19235 0.84 +0.2 74,008 + IAI47_19235 0.84 -1.4 74,014 + IAI47_19235 0.85 -0.0 74,034 + IAI47_19235 0.88 +0.8 74,034 + IAI47_19235 0.88 +0.7 74,034 + IAI47_19235 0.88 +0.4 74,034 + IAI47_19235 0.88 +1.7 74,035 - IAI47_19235 0.88 -0.1 74,055 + +0.5 74,056 - +0.4 74,142 + +0.2 74,142 + +0.2 74,142 + +1.3 74,142 + -0.6 74,143 - -0.2 74,145 + +0.2 74,145 + +2.8 74,145 + -2.5 74,146 - +0.6 74,146 - +0.8 74,146 - +1.0 74,161 + -1.1 74,162 - +0.7 74,162 - +0.9 74,162 - +0.3 74,162 - -1.4 74,162 - -0.9 74,164 + -0.9 74,228 - +1.4 74,228 - -0.4 74,250 + -0.0 74,251 - +0.0 74,333 + IAI47_19240 0.15 +2.3 74,333 + IAI47_19240 0.15 +0.9 74,334 - IAI47_19240 0.15 +0.3 74,334 - IAI47_19240 0.15 +0.9 74,412 + IAI47_19240 0.25 -1.3 74,413 - IAI47_19240 0.25 -0.8 74,433 + IAI47_19240 0.28 +1.0 74,433 + IAI47_19240 0.28 +1.4 74,433 + IAI47_19240 0.28 +1.9 74,434 - IAI47_19240 0.28 +0.0 74,434 - IAI47_19240 0.28 -1.2 74,434 - IAI47_19240 0.28 +0.6 74,443 + IAI47_19240 0.29 -0.7 74,444 - IAI47_19240 0.29 -1.6 74,446 + IAI47_19240 0.30 -1.5 74,447 - IAI47_19240 0.30 -1.8 74,470 + IAI47_19240 0.33 -0.7 74,512 + IAI47_19240 0.39 +2.6 74,524 + IAI47_19240 0.40 -1.3 74,524 + IAI47_19240 0.40 -0.3 74,527 + IAI47_19240 0.41 -0.1 74,528 - IAI47_19240 0.41 +1.6 74,528 - IAI47_19240 0.41 +0.2 74,545 + IAI47_19240 0.43 +1.1 74,574 + IAI47_19240 0.47 -0.9 74,578 + IAI47_19240 0.47 -0.1 74,578 + IAI47_19240 0.47 -1.5 74,597 + IAI47_19240 0.50 -1.0 74,597 + IAI47_19240 0.50 -1.1 74,597 + IAI47_19240 0.50 +0.1 74,633 + IAI47_19240 0.55 +2.3 74,634 - IAI47_19240 0.55 +2.1 74,634 - IAI47_19240 0.55 -0.2 74,635 + IAI47_19240 0.55 -2.6 74,635 + IAI47_19240 0.55 +0.5 74,635 + IAI47_19240 0.55 +0.2 74,635 + IAI47_19240 0.55 +0.3 74,635 + IAI47_19240 0.55 +0.6 74,635 + IAI47_19240 0.55 -0.1 74,636 - IAI47_19240 0.55 +0.5 74,682 - IAI47_19240 0.61 -1.8 74,692 + IAI47_19240 0.62 -0.7 74,692 + IAI47_19240 0.62 +0.2 74,693 - IAI47_19240 0.63 -0.1 74,715 + IAI47_19240 0.65 -1.1 74,716 - IAI47_19240 0.66 +1.9 74,806 + IAI47_19240 0.78 -0.1 74,806 + IAI47_19240 0.78 -1.1 74,806 + IAI47_19240 0.78 +1.3 74,806 + IAI47_19240 0.78 +1.1 74,807 - IAI47_19240 0.78 +0.8 74,810 + IAI47_19240 0.78 +0.1 74,811 - IAI47_19240 0.78 +0.1 74,815 + IAI47_19240 0.79 -0.2 74,815 + IAI47_19240 0.79 +1.1 74,815 + IAI47_19240 0.79 +1.8 74,816 - IAI47_19240 0.79 +1.4 74,911 + +1.1 74,911 + -1.4 74,911 + +2.8 74,912 - -0.8 74,924 + -0.0 74,925 - -1.1 74,947 + +1.6 74,948 - +1.1 74,983 + +0.5 75,134 + IAI47_19245 0.26 +0.8 75,264 - IAI47_19245 0.49 +2.8 75,264 - IAI47_19245 0.49 +1.5 75,270 + -1.9 75,270 + IAI47_19245 0.50 -0.9 75,271 - IAI47_19245 0.50 -0.1 75,271 - IAI47_19245 0.50 +1.9 75,271 - IAI47_19245 0.50 +0.1 75,271 - IAI47_19245 0.50 +4.9 75,366 - IAI47_19245 0.67 +0.6 75,466 + IAI47_19245 0.85 -0.8 75,466 + IAI47_19245 0.85 -0.7 75,467 - IAI47_19245 0.85 +3.2 75,467 - IAI47_19245 0.85 -1.4 75,467 - IAI47_19245 0.85 +0.7 75,467 - IAI47_19245 0.85 -0.3 75,479 + IAI47_19245 0.87 +0.3 75,479 + IAI47_19245 0.87 -0.2 75,479 + IAI47_19245 0.87 +0.6 75,480 - IAI47_19245 0.88 -1.0 75,480 - IAI47_19245 0.88 +0.4 75,548 - -0.6 75,550 + +0.6 75,550 + -0.6 75,586 + -2.4 75,586 + +1.0 75,608 + +0.8 75,608 + +0.5 75,608 + +0.5 75,608 + -0.1 75,608 + +0.2 75,609 - +1.3 75,609 - -2.0 75,609 - -0.5 75,622 + -0.9 75,623 - -1.6 75,623 - -0.9 75,638 + -0.4 75,639 - +1.3 75,665 + +1.1 75,666 - +0.8 75,666 - -0.9 75,666 - +0.0 75,671 + +1.3 75,672 - +0.8 75,672 - +0.5 75,672 - +0.1 75,689 + +0.3 75,708 + +0.4 75,708 + +0.6 75,709 - +0.7 75,709 - -0.7 75,747 + -1.1 75,748 - +0.6 75,767 + +1.9 75,767 + +0.8 75,767 + +0.5 75,768 - +2.6 75,773 + -1.7 75,773 + -0.9 75,774 - -1.5 75,774 - +1.0 75,810 + -0.8 75,823 + +0.1 75,824 - -0.2 75,876 - -0.5 75,930 - -0.5 75,935 + -0.8 76,013 + IAI47_19250 0.13 +0.8 76,013 + IAI47_19250 0.13 +0.0 76,014 - IAI47_19250 0.13 +1.0 76,014 - IAI47_19250 0.13 +0.9 76,025 + IAI47_19250 0.13 +1.5 76,026 - IAI47_19250 0.14 +0.6 76,075 - IAI47_19250 0.17 -0.4 76,083 - IAI47_19250 0.17 +0.9 76,083 - IAI47_19250 0.17 -0.1 76,146 - -0.7 76,146 - IAI47_19250 0.21 +1.1 76,167 + IAI47_19250 0.23 -0.6 76,167 + IAI47_19250 0.23 +0.8 76,167 + IAI47_19250 0.23 -1.1 76,167 + IAI47_19250 0.23 -0.7 76,167 + IAI47_19250 0.23 -0.3 76,168 - IAI47_19250 0.23 +0.5 76,168 - IAI47_19250 0.23 +1.3 76,168 - IAI47_19250 0.23 +0.3 76,168 - IAI47_19250 0.23 -0.2 76,173 - IAI47_19250 0.23 +0.6 76,276 + IAI47_19250 0.30 -0.3 76,276 + IAI47_19250 0.30 +0.3 76,276 + IAI47_19250 0.30 +1.5 76,276 + IAI47_19250 0.30 -0.8 76,277 - IAI47_19250 0.30 +0.5 76,277 - IAI47_19250 0.30 -0.7 76,295 + IAI47_19250 0.31 +1.2 76,295 + IAI47_19250 0.31 +1.3 76,295 + IAI47_19250 0.31 +0.7 76,327 + IAI47_19250 0.33 +0.3 76,327 + IAI47_19250 0.33 +2.6 76,327 + IAI47_19250 0.33 +0.3 76,372 + IAI47_19250 0.36 -1.6 76,379 + IAI47_19250 0.37 -0.5 76,380 - IAI47_19250 0.37 -0.2 76,380 - IAI47_19250 0.37 -0.5 76,392 + IAI47_19250 0.38 -1.4 76,393 - IAI47_19250 0.38 +1.5 76,393 - IAI47_19250 0.38 -0.2 76,515 + IAI47_19250 0.46 +0.0 76,516 - IAI47_19250 0.46 +1.5 76,535 + IAI47_19250 0.47 +0.3 76,535 + IAI47_19250 0.47 -0.7 76,535 + IAI47_19250 0.47 -2.7 76,535 + IAI47_19250 0.47 +1.0 76,536 - IAI47_19250 0.47 -1.0 76,536 - IAI47_19250 0.47 -0.4 76,536 - IAI47_19250 0.47 -0.5 76,539 + IAI47_19250 0.48 +0.5 76,539 + IAI47_19250 0.48 -0.8 76,539 + IAI47_19250 0.48 +1.1 76,540 - IAI47_19250 0.48 +0.3 76,544 + IAI47_19250 0.48 +0.2 76,545 - IAI47_19250 0.48 +0.6
Or see this region's nucleotide sequence