Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_14440 and IAI47_14445 are separated by 113 nucleotides IAI47_14445 and IAI47_14450 are separated by 105 nucleotides
IAI47_14440: IAI47_14440 - ferrochelatase, at 3,050,756 to 3,051,715
_14440
IAI47_14445: IAI47_14445 - adenylate kinase, at 3,051,829 to 3,052,473
_14445
IAI47_14450: IAI47_14450 - hypothetical protein, at 3,052,579 to 3,053,463
_14450
Position (kb)
3051
3052
3053 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3050.982 kb on + strand, within IAI47_14440 at 3050.982 kb on + strand, within IAI47_14440 at 3050.982 kb on + strand, within IAI47_14440 at 3050.983 kb on - strand, within IAI47_14440 at 3050.983 kb on - strand, within IAI47_14440 at 3050.983 kb on - strand, within IAI47_14440 at 3051.033 kb on + strand, within IAI47_14440 at 3051.033 kb on + strand, within IAI47_14440 at 3051.033 kb on + strand, within IAI47_14440 at 3051.033 kb on + strand, within IAI47_14440 at 3051.034 kb on - strand, within IAI47_14440 at 3051.034 kb on - strand, within IAI47_14440 at 3051.034 kb on - strand, within IAI47_14440 at 3051.034 kb on - strand, within IAI47_14440 at 3051.061 kb on + strand, within IAI47_14440 at 3051.062 kb on - strand, within IAI47_14440 at 3051.062 kb on - strand, within IAI47_14440 at 3051.147 kb on - strand, within IAI47_14440 at 3051.215 kb on - strand, within IAI47_14440 at 3051.215 kb on - strand, within IAI47_14440 at 3051.216 kb on + strand, within IAI47_14440 at 3051.216 kb on + strand, within IAI47_14440 at 3051.216 kb on + strand, within IAI47_14440 at 3051.217 kb on - strand, within IAI47_14440 at 3051.234 kb on + strand, within IAI47_14440 at 3051.234 kb on + strand, within IAI47_14440 at 3051.270 kb on + strand, within IAI47_14440 at 3051.270 kb on + strand, within IAI47_14440 at 3051.271 kb on - strand, within IAI47_14440 at 3051.271 kb on - strand, within IAI47_14440 at 3051.382 kb on + strand, within IAI47_14440 at 3051.383 kb on - strand, within IAI47_14440 at 3051.383 kb on - strand, within IAI47_14440 at 3051.414 kb on + strand, within IAI47_14440 at 3051.414 kb on + strand, within IAI47_14440 at 3051.414 kb on + strand, within IAI47_14440 at 3051.414 kb on + strand, within IAI47_14440 at 3051.414 kb on + strand, within IAI47_14440 at 3051.414 kb on + strand, within IAI47_14440 at 3051.414 kb on + strand, within IAI47_14440 at 3051.414 kb on + strand, within IAI47_14440 at 3051.415 kb on - strand, within IAI47_14440 at 3051.415 kb on - strand, within IAI47_14440 at 3051.415 kb on - strand, within IAI47_14440 at 3051.415 kb on - strand, within IAI47_14440 at 3051.415 kb on - strand, within IAI47_14440 at 3051.415 kb on - strand, within IAI47_14440 at 3051.415 kb on - strand, within IAI47_14440 at 3051.415 kb on - strand, within IAI47_14440 at 3051.439 kb on + strand, within IAI47_14440 at 3051.439 kb on + strand, within IAI47_14440 at 3051.440 kb on - strand, within IAI47_14440 at 3051.440 kb on - strand, within IAI47_14440 at 3051.440 kb on - strand, within IAI47_14440 at 3051.440 kb on - strand, within IAI47_14440 at 3051.481 kb on - strand, within IAI47_14440 at 3051.481 kb on - strand, within IAI47_14440 at 3051.488 kb on + strand, within IAI47_14440 at 3051.522 kb on + strand, within IAI47_14440 at 3051.522 kb on + strand, within IAI47_14440 at 3051.530 kb on - strand, within IAI47_14440 at 3051.530 kb on - strand, within IAI47_14440 at 3051.636 kb on + strand at 3051.643 kb on + strand at 3051.644 kb on - strand at 3051.644 kb on - strand at 3051.673 kb on + strand at 3051.678 kb on - strand at 3051.678 kb on - strand at 3051.678 kb on - strand at 3051.690 kb on - strand at 3051.703 kb on + strand at 3051.703 kb on + strand at 3051.703 kb on + strand at 3051.703 kb on + strand at 3051.704 kb on - strand at 3051.704 kb on - strand at 3051.771 kb on + strand at 3051.771 kb on + strand at 3051.781 kb on + strand at 3051.791 kb on + strand at 3051.810 kb on - strand at 3051.810 kb on - strand at 3051.810 kb on - strand at 3051.813 kb on - strand at 3051.844 kb on - strand at 3051.844 kb on - strand at 3052.638 kb on + strand at 3052.639 kb on - strand at 3052.689 kb on + strand, within IAI47_14450 at 3052.689 kb on + strand, within IAI47_14450 at 3052.689 kb on + strand, within IAI47_14450 at 3052.690 kb on - strand, within IAI47_14450 at 3052.690 kb on - strand, within IAI47_14450 at 3052.690 kb on - strand, within IAI47_14450 at 3052.690 kb on - strand, within IAI47_14450 at 3052.722 kb on + strand, within IAI47_14450 at 3052.722 kb on + strand, within IAI47_14450 at 3052.722 kb on + strand, within IAI47_14450 at 3052.722 kb on + strand, within IAI47_14450 at 3052.722 kb on + strand, within IAI47_14450 at 3052.723 kb on - strand, within IAI47_14450 at 3052.723 kb on - strand, within IAI47_14450 at 3052.809 kb on + strand, within IAI47_14450 at 3052.809 kb on + strand, within IAI47_14450 at 3052.809 kb on + strand, within IAI47_14450 at 3052.810 kb on - strand, within IAI47_14450 at 3052.898 kb on + strand, within IAI47_14450 at 3052.898 kb on + strand, within IAI47_14450 at 3052.898 kb on + strand, within IAI47_14450 at 3052.899 kb on - strand, within IAI47_14450 at 3052.940 kb on + strand, within IAI47_14450 at 3052.940 kb on + strand, within IAI47_14450 at 3052.940 kb on + strand, within IAI47_14450 at 3052.940 kb on + strand, within IAI47_14450 at 3052.940 kb on + strand, within IAI47_14450 at 3052.950 kb on + strand, within IAI47_14450 at 3052.951 kb on - strand, within IAI47_14450 at 3052.951 kb on - strand, within IAI47_14450 at 3052.951 kb on - strand, within IAI47_14450 at 3052.951 kb on - strand, within IAI47_14450 at 3052.972 kb on + strand, within IAI47_14450 at 3052.973 kb on - strand, within IAI47_14450 at 3052.998 kb on + strand, within IAI47_14450 at 3052.999 kb on - strand, within IAI47_14450 at 3053.041 kb on - strand, within IAI47_14450 at 3053.041 kb on - strand, within IAI47_14450 at 3053.045 kb on - strand, within IAI47_14450 at 3053.072 kb on - strand at 3053.164 kb on + strand, within IAI47_14450 at 3053.164 kb on + strand, within IAI47_14450 at 3053.173 kb on + strand, within IAI47_14450 at 3053.173 kb on + strand, within IAI47_14450 at 3053.174 kb on - strand, within IAI47_14450 at 3053.174 kb on - strand, within IAI47_14450 at 3053.196 kb on + strand, within IAI47_14450 at 3053.196 kb on + strand, within IAI47_14450 at 3053.196 kb on + strand, within IAI47_14450 at 3053.196 kb on + strand, within IAI47_14450 at 3053.197 kb on - strand, within IAI47_14450 at 3053.197 kb on - strand, within IAI47_14450 at 3053.197 kb on - strand, within IAI47_14450 at 3053.211 kb on + strand, within IAI47_14450 at 3053.211 kb on + strand, within IAI47_14450 at 3053.211 kb on + strand, within IAI47_14450 at 3053.211 kb on + strand, within IAI47_14450 at 3053.211 kb on + strand, within IAI47_14450 at 3053.211 kb on + strand, within IAI47_14450 at 3053.211 kb on + strand, within IAI47_14450 at 3053.212 kb on - strand, within IAI47_14450 at 3053.212 kb on - strand, within IAI47_14450 at 3053.212 kb on - strand, within IAI47_14450 at 3053.212 kb on - strand, within IAI47_14450 at 3053.212 kb on - strand, within IAI47_14450 at 3053.212 kb on - strand, within IAI47_14450 at 3053.212 kb on - strand, within IAI47_14450 at 3053.224 kb on + strand, within IAI47_14450 at 3053.224 kb on + strand, within IAI47_14450 at 3053.247 kb on + strand, within IAI47_14450 at 3053.248 kb on - strand, within IAI47_14450 at 3053.250 kb on + strand, within IAI47_14450 at 3053.250 kb on + strand, within IAI47_14450 at 3053.250 kb on + strand, within IAI47_14450 at 3053.250 kb on + strand, within IAI47_14450 at 3053.250 kb on + strand, within IAI47_14450 at 3053.250 kb on + strand, within IAI47_14450 at 3053.250 kb on + strand, within IAI47_14450 at 3053.251 kb on - strand, within IAI47_14450 at 3053.251 kb on - strand, within IAI47_14450 at 3053.251 kb on - strand, within IAI47_14450 at 3053.251 kb on - strand, within IAI47_14450 at 3053.251 kb on - strand, within IAI47_14450 at 3053.292 kb on + strand, within IAI47_14450 at 3053.292 kb on + strand, within IAI47_14450 at 3053.292 kb on + strand, within IAI47_14450 at 3053.292 kb on + strand, within IAI47_14450 at 3053.293 kb on - strand, within IAI47_14450 at 3053.341 kb on + strand, within IAI47_14450 at 3053.342 kb on - strand, within IAI47_14450 at 3053.446 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 3,050,982 + IAI47_14440 0.24 -0.6 3,050,982 + IAI47_14440 0.24 +0.2 3,050,982 + IAI47_14440 0.24 -0.2 3,050,983 - IAI47_14440 0.24 +0.7 3,050,983 - IAI47_14440 0.24 -0.8 3,050,983 - IAI47_14440 0.24 -1.5 3,051,033 + IAI47_14440 0.29 -1.1 3,051,033 + IAI47_14440 0.29 -0.6 3,051,033 + IAI47_14440 0.29 -0.4 3,051,033 + IAI47_14440 0.29 +0.0 3,051,034 - IAI47_14440 0.29 -1.0 3,051,034 - IAI47_14440 0.29 +0.2 3,051,034 - IAI47_14440 0.29 -0.0 3,051,034 - IAI47_14440 0.29 -0.0 3,051,061 + IAI47_14440 0.32 +0.3 3,051,062 - IAI47_14440 0.32 +2.1 3,051,062 - IAI47_14440 0.32 -0.9 3,051,147 - IAI47_14440 0.41 -0.5 3,051,215 - IAI47_14440 0.48 -1.2 3,051,215 - IAI47_14440 0.48 -0.6 3,051,216 + IAI47_14440 0.48 -1.6 3,051,216 + IAI47_14440 0.48 +0.4 3,051,216 + IAI47_14440 0.48 -1.4 3,051,217 - IAI47_14440 0.48 +0.0 3,051,234 + IAI47_14440 0.50 -0.0 3,051,234 + IAI47_14440 0.50 +1.0 3,051,270 + IAI47_14440 0.54 +1.3 3,051,270 + IAI47_14440 0.54 -1.2 3,051,271 - IAI47_14440 0.54 +0.5 3,051,271 - IAI47_14440 0.54 +0.0 3,051,382 + IAI47_14440 0.65 -1.0 3,051,383 - IAI47_14440 0.65 -0.3 3,051,383 - IAI47_14440 0.65 +0.5 3,051,414 + IAI47_14440 0.69 -0.7 3,051,414 + IAI47_14440 0.69 -1.2 3,051,414 + IAI47_14440 0.69 +1.3 3,051,414 + IAI47_14440 0.69 -1.0 3,051,414 + IAI47_14440 0.69 -0.9 3,051,414 + IAI47_14440 0.69 +0.5 3,051,414 + IAI47_14440 0.69 +0.6 3,051,414 + IAI47_14440 0.69 -1.6 3,051,415 - IAI47_14440 0.69 -0.7 3,051,415 - IAI47_14440 0.69 -0.8 3,051,415 - IAI47_14440 0.69 -1.3 3,051,415 - IAI47_14440 0.69 -0.2 3,051,415 - IAI47_14440 0.69 +1.6 3,051,415 - IAI47_14440 0.69 -0.6 3,051,415 - IAI47_14440 0.69 +0.7 3,051,415 - IAI47_14440 0.69 +1.7 3,051,439 + IAI47_14440 0.71 +0.2 3,051,439 + IAI47_14440 0.71 +1.1 3,051,440 - IAI47_14440 0.71 -0.1 3,051,440 - IAI47_14440 0.71 -1.1 3,051,440 - IAI47_14440 0.71 +1.8 3,051,440 - IAI47_14440 0.71 +0.0 3,051,481 - IAI47_14440 0.76 -1.4 3,051,481 - IAI47_14440 0.76 +0.1 3,051,488 + IAI47_14440 0.76 +2.0 3,051,522 + IAI47_14440 0.80 +0.3 3,051,522 + IAI47_14440 0.80 +1.7 3,051,530 - IAI47_14440 0.81 -0.4 3,051,530 - IAI47_14440 0.81 -1.0 3,051,636 + -1.3 3,051,643 + +1.0 3,051,644 - +0.5 3,051,644 - -2.0 3,051,673 + +0.2 3,051,678 - +0.2 3,051,678 - +0.1 3,051,678 - -0.6 3,051,690 - -0.6 3,051,703 + +0.5 3,051,703 + -0.8 3,051,703 + -1.9 3,051,703 + +1.2 3,051,704 - +1.6 3,051,704 - +0.0 3,051,771 + -1.5 3,051,771 + +2.2 3,051,781 + +0.9 3,051,791 + -0.1 3,051,810 - +0.1 3,051,810 - -0.1 3,051,810 - +1.1 3,051,813 - +1.6 3,051,844 - -1.1 3,051,844 - +0.4 3,052,638 + +0.5 3,052,639 - +0.8 3,052,689 + IAI47_14450 0.12 -0.2 3,052,689 + IAI47_14450 0.12 -0.2 3,052,689 + IAI47_14450 0.12 -0.3 3,052,690 - IAI47_14450 0.13 +2.1 3,052,690 - IAI47_14450 0.13 -1.4 3,052,690 - IAI47_14450 0.13 +0.2 3,052,690 - IAI47_14450 0.13 +1.2 3,052,722 + IAI47_14450 0.16 -0.4 3,052,722 + IAI47_14450 0.16 +0.3 3,052,722 + IAI47_14450 0.16 +0.0 3,052,722 + IAI47_14450 0.16 -2.1 3,052,722 + IAI47_14450 0.16 -1.3 3,052,723 - IAI47_14450 0.16 -0.6 3,052,723 - IAI47_14450 0.16 -1.0 3,052,809 + IAI47_14450 0.26 +1.3 3,052,809 + IAI47_14450 0.26 -0.4 3,052,809 + IAI47_14450 0.26 -1.7 3,052,810 - IAI47_14450 0.26 +0.8 3,052,898 + IAI47_14450 0.36 +0.7 3,052,898 + IAI47_14450 0.36 +1.1 3,052,898 + IAI47_14450 0.36 -0.4 3,052,899 - IAI47_14450 0.36 +1.9 3,052,940 + IAI47_14450 0.41 +0.5 3,052,940 + IAI47_14450 0.41 -0.2 3,052,940 + IAI47_14450 0.41 -1.1 3,052,940 + IAI47_14450 0.41 -0.8 3,052,940 + IAI47_14450 0.41 +0.2 3,052,950 + IAI47_14450 0.42 -1.0 3,052,951 - IAI47_14450 0.42 -0.6 3,052,951 - IAI47_14450 0.42 -2.4 3,052,951 - IAI47_14450 0.42 +0.4 3,052,951 - IAI47_14450 0.42 -1.6 3,052,972 + IAI47_14450 0.44 -0.1 3,052,973 - IAI47_14450 0.45 +0.2 3,052,998 + IAI47_14450 0.47 +1.3 3,052,999 - IAI47_14450 0.47 +1.8 3,053,041 - IAI47_14450 0.52 -0.4 3,053,041 - IAI47_14450 0.52 +1.0 3,053,045 - IAI47_14450 0.53 +1.6 3,053,072 - +2.4 3,053,164 + IAI47_14450 0.66 -0.1 3,053,164 + IAI47_14450 0.66 +0.0 3,053,173 + IAI47_14450 0.67 +2.1 3,053,173 + IAI47_14450 0.67 +0.2 3,053,174 - IAI47_14450 0.67 +2.1 3,053,174 - IAI47_14450 0.67 -0.9 3,053,196 + IAI47_14450 0.70 -1.3 3,053,196 + IAI47_14450 0.70 -1.2 3,053,196 + IAI47_14450 0.70 -0.4 3,053,196 + IAI47_14450 0.70 -0.2 3,053,197 - IAI47_14450 0.70 -0.1 3,053,197 - IAI47_14450 0.70 +0.2 3,053,197 - IAI47_14450 0.70 -0.9 3,053,211 + IAI47_14450 0.71 +0.6 3,053,211 + IAI47_14450 0.71 +0.1 3,053,211 + IAI47_14450 0.71 -1.0 3,053,211 + IAI47_14450 0.71 +2.4 3,053,211 + IAI47_14450 0.71 -1.5 3,053,211 + IAI47_14450 0.71 -0.4 3,053,211 + IAI47_14450 0.71 +1.6 3,053,212 - IAI47_14450 0.72 -1.1 3,053,212 - IAI47_14450 0.72 +0.7 3,053,212 - IAI47_14450 0.72 -1.0 3,053,212 - IAI47_14450 0.72 -0.4 3,053,212 - IAI47_14450 0.72 +0.9 3,053,212 - IAI47_14450 0.72 -0.1 3,053,212 - IAI47_14450 0.72 +1.9 3,053,224 + IAI47_14450 0.73 -0.1 3,053,224 + IAI47_14450 0.73 -0.4 3,053,247 + IAI47_14450 0.75 -2.1 3,053,248 - IAI47_14450 0.76 +0.1 3,053,250 + IAI47_14450 0.76 +2.4 3,053,250 + IAI47_14450 0.76 +3.0 3,053,250 + IAI47_14450 0.76 +0.6 3,053,250 + IAI47_14450 0.76 -0.3 3,053,250 + IAI47_14450 0.76 +0.7 3,053,250 + IAI47_14450 0.76 +1.5 3,053,250 + IAI47_14450 0.76 -1.8 3,053,251 - IAI47_14450 0.76 +0.4 3,053,251 - IAI47_14450 0.76 +0.6 3,053,251 - IAI47_14450 0.76 -0.1 3,053,251 - IAI47_14450 0.76 -1.1 3,053,251 - IAI47_14450 0.76 -2.6 3,053,292 + IAI47_14450 0.81 -0.3 3,053,292 + IAI47_14450 0.81 +0.3 3,053,292 + IAI47_14450 0.81 +0.0 3,053,292 + IAI47_14450 0.81 +0.0 3,053,293 - IAI47_14450 0.81 -0.9 3,053,341 + IAI47_14450 0.86 -0.4 3,053,342 - IAI47_14450 0.86 -1.1 3,053,446 + +0.0
Or see this region's nucleotide sequence