Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20055 and IAI47_20060 overlap by 4 nucleotides IAI47_20060 and IAI47_20065 are separated by 156 nucleotides IAI47_20065 and IAI47_20070 are separated by 74 nucleotides
IAI47_20055: IAI47_20055 - non-canonical purine NTP pyrophosphatase, at 243,196 to 243,756
_20055
IAI47_20060: IAI47_20060 - SIR2 family protein, at 243,753 to 244,547
_20060
IAI47_20065: IAI47_20065 - YebG family protein, at 244,704 to 245,045
_20065
IAI47_20070: IAI47_20070 - Fic family protein, at 245,120 to 246,253
_20070
Position (kb)
244
245
246 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 243.724 kb on - strand at 243.731 kb on + strand at 243.732 kb on - strand at 243.768 kb on - strand at 243.768 kb on - strand at 243.768 kb on - strand at 243.778 kb on - strand at 243.778 kb on - strand at 243.816 kb on - strand at 243.882 kb on + strand, within IAI47_20060 at 243.882 kb on + strand, within IAI47_20060 at 243.902 kb on + strand, within IAI47_20060 at 243.902 kb on + strand, within IAI47_20060 at 243.902 kb on + strand, within IAI47_20060 at 243.902 kb on + strand, within IAI47_20060 at 243.902 kb on + strand, within IAI47_20060 at 243.902 kb on + strand, within IAI47_20060 at 243.902 kb on + strand, within IAI47_20060 at 243.903 kb on - strand, within IAI47_20060 at 243.903 kb on - strand, within IAI47_20060 at 243.903 kb on - strand, within IAI47_20060 at 243.903 kb on - strand, within IAI47_20060 at 243.906 kb on + strand, within IAI47_20060 at 243.906 kb on + strand, within IAI47_20060 at 243.906 kb on + strand, within IAI47_20060 at 243.907 kb on - strand, within IAI47_20060 at 243.907 kb on - strand, within IAI47_20060 at 243.936 kb on + strand, within IAI47_20060 at 243.936 kb on + strand, within IAI47_20060 at 243.936 kb on + strand, within IAI47_20060 at 243.936 kb on + strand, within IAI47_20060 at 243.936 kb on + strand, within IAI47_20060 at 243.936 kb on + strand, within IAI47_20060 at 243.937 kb on - strand, within IAI47_20060 at 243.937 kb on - strand, within IAI47_20060 at 243.937 kb on - strand, within IAI47_20060 at 243.937 kb on - strand, within IAI47_20060 at 243.937 kb on - strand, within IAI47_20060 at 243.937 kb on - strand, within IAI47_20060 at 244.031 kb on + strand, within IAI47_20060 at 244.032 kb on - strand, within IAI47_20060 at 244.032 kb on - strand, within IAI47_20060 at 244.040 kb on + strand, within IAI47_20060 at 244.040 kb on + strand, within IAI47_20060 at 244.066 kb on - strand, within IAI47_20060 at 244.066 kb on - strand, within IAI47_20060 at 244.073 kb on + strand, within IAI47_20060 at 244.073 kb on + strand, within IAI47_20060 at 244.073 kb on + strand, within IAI47_20060 at 244.074 kb on - strand, within IAI47_20060 at 244.074 kb on - strand at 244.074 kb on - strand, within IAI47_20060 at 244.077 kb on + strand, within IAI47_20060 at 244.077 kb on + strand, within IAI47_20060 at 244.077 kb on + strand, within IAI47_20060 at 244.078 kb on - strand, within IAI47_20060 at 244.078 kb on - strand, within IAI47_20060 at 244.078 kb on - strand, within IAI47_20060 at 244.122 kb on + strand, within IAI47_20060 at 244.122 kb on + strand, within IAI47_20060 at 244.122 kb on + strand, within IAI47_20060 at 244.122 kb on + strand, within IAI47_20060 at 244.123 kb on - strand, within IAI47_20060 at 244.123 kb on - strand, within IAI47_20060 at 244.123 kb on - strand, within IAI47_20060 at 244.123 kb on - strand, within IAI47_20060 at 244.123 kb on - strand, within IAI47_20060 at 244.123 kb on - strand, within IAI47_20060 at 244.123 kb on - strand, within IAI47_20060 at 244.125 kb on + strand, within IAI47_20060 at 244.125 kb on + strand, within IAI47_20060 at 244.125 kb on + strand, within IAI47_20060 at 244.125 kb on + strand, within IAI47_20060 at 244.126 kb on - strand, within IAI47_20060 at 244.127 kb on + strand, within IAI47_20060 at 244.127 kb on + strand, within IAI47_20060 at 244.127 kb on + strand, within IAI47_20060 at 244.128 kb on - strand, within IAI47_20060 at 244.128 kb on - strand, within IAI47_20060 at 244.128 kb on - strand, within IAI47_20060 at 244.128 kb on - strand, within IAI47_20060 at 244.128 kb on - strand, within IAI47_20060 at 244.169 kb on + strand, within IAI47_20060 at 244.170 kb on - strand, within IAI47_20060 at 244.239 kb on + strand, within IAI47_20060 at 244.239 kb on + strand, within IAI47_20060 at 244.277 kb on + strand, within IAI47_20060 at 244.277 kb on + strand, within IAI47_20060 at 244.278 kb on - strand, within IAI47_20060 at 244.278 kb on - strand, within IAI47_20060 at 244.278 kb on - strand, within IAI47_20060 at 244.278 kb on - strand, within IAI47_20060 at 244.416 kb on + strand, within IAI47_20060 at 244.417 kb on - strand, within IAI47_20060 at 244.433 kb on + strand, within IAI47_20060 at 244.434 kb on - strand, within IAI47_20060 at 244.434 kb on - strand, within IAI47_20060 at 244.434 kb on - strand, within IAI47_20060 at 244.546 kb on - strand at 244.546 kb on - strand at 244.546 kb on - strand at 244.636 kb on - strand at 244.636 kb on - strand at 244.636 kb on - strand at 244.636 kb on - strand at 244.636 kb on - strand at 244.668 kb on - strand at 244.729 kb on + strand at 244.766 kb on + strand, within IAI47_20065 at 244.767 kb on - strand, within IAI47_20065 at 244.831 kb on + strand, within IAI47_20065 at 244.831 kb on + strand, within IAI47_20065 at 244.831 kb on + strand, within IAI47_20065 at 244.832 kb on - strand, within IAI47_20065 at 244.832 kb on - strand, within IAI47_20065 at 244.913 kb on + strand, within IAI47_20065 at 244.913 kb on + strand, within IAI47_20065 at 244.915 kb on - strand, within IAI47_20065 at 245.043 kb on + strand at 245.121 kb on + strand at 245.128 kb on + strand at 245.129 kb on - strand at 245.132 kb on + strand at 245.132 kb on + strand at 245.133 kb on - strand at 245.133 kb on - strand at 245.133 kb on - strand at 245.133 kb on - strand at 245.239 kb on + strand, within IAI47_20070 at 245.239 kb on + strand, within IAI47_20070 at 245.240 kb on - strand, within IAI47_20070 at 245.243 kb on + strand, within IAI47_20070 at 245.244 kb on - strand, within IAI47_20070 at 245.276 kb on + strand, within IAI47_20070 at 245.330 kb on + strand, within IAI47_20070 at 245.331 kb on - strand, within IAI47_20070 at 245.331 kb on - strand, within IAI47_20070 at 245.345 kb on + strand, within IAI47_20070 at 245.345 kb on + strand, within IAI47_20070 at 245.345 kb on + strand, within IAI47_20070 at 245.346 kb on - strand, within IAI47_20070 at 245.346 kb on - strand, within IAI47_20070 at 245.367 kb on - strand, within IAI47_20070 at 245.468 kb on - strand, within IAI47_20070 at 245.468 kb on - strand, within IAI47_20070 at 245.468 kb on - strand, within IAI47_20070 at 245.506 kb on - strand, within IAI47_20070 at 245.506 kb on - strand, within IAI47_20070 at 245.528 kb on + strand, within IAI47_20070 at 245.528 kb on + strand, within IAI47_20070 at 245.547 kb on + strand, within IAI47_20070 at 245.548 kb on - strand, within IAI47_20070 at 245.608 kb on + strand, within IAI47_20070 at 245.636 kb on + strand, within IAI47_20070 at 245.637 kb on - strand, within IAI47_20070 at 245.637 kb on - strand, within IAI47_20070 at 245.637 kb on - strand, within IAI47_20070 at 245.676 kb on - strand, within IAI47_20070 at 245.789 kb on + strand, within IAI47_20070 at 245.789 kb on + strand, within IAI47_20070 at 245.789 kb on + strand, within IAI47_20070 at 245.789 kb on + strand, within IAI47_20070 at 245.790 kb on - strand, within IAI47_20070 at 245.790 kb on - strand, within IAI47_20070 at 245.790 kb on - strand, within IAI47_20070 at 245.852 kb on + strand, within IAI47_20070 at 245.853 kb on - strand, within IAI47_20070 at 245.853 kb on - strand, within IAI47_20070 at 245.853 kb on - strand, within IAI47_20070 at 245.853 kb on - strand, within IAI47_20070 at 245.853 kb on - strand, within IAI47_20070 at 245.853 kb on - strand, within IAI47_20070 at 245.898 kb on - strand, within IAI47_20070 at 245.985 kb on + strand, within IAI47_20070
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 243,724 - +3.3 243,731 + -0.9 243,732 - -0.0 243,768 - +0.2 243,768 - -1.4 243,768 - +0.4 243,778 - +0.9 243,778 - -0.9 243,816 - +0.9 243,882 + IAI47_20060 0.16 +1.0 243,882 + IAI47_20060 0.16 -0.1 243,902 + IAI47_20060 0.19 -0.8 243,902 + IAI47_20060 0.19 +0.8 243,902 + IAI47_20060 0.19 +0.1 243,902 + IAI47_20060 0.19 -1.8 243,902 + IAI47_20060 0.19 -0.9 243,902 + IAI47_20060 0.19 +0.2 243,902 + IAI47_20060 0.19 +0.1 243,903 - IAI47_20060 0.19 +1.8 243,903 - IAI47_20060 0.19 +0.2 243,903 - IAI47_20060 0.19 +0.9 243,903 - IAI47_20060 0.19 -0.6 243,906 + IAI47_20060 0.19 +0.1 243,906 + IAI47_20060 0.19 +0.6 243,906 + IAI47_20060 0.19 +0.1 243,907 - IAI47_20060 0.19 +0.7 243,907 - IAI47_20060 0.19 -0.4 243,936 + IAI47_20060 0.23 -0.4 243,936 + IAI47_20060 0.23 -0.5 243,936 + IAI47_20060 0.23 -1.1 243,936 + IAI47_20060 0.23 +1.3 243,936 + IAI47_20060 0.23 -0.3 243,936 + IAI47_20060 0.23 -0.1 243,937 - IAI47_20060 0.23 -1.6 243,937 - IAI47_20060 0.23 +1.3 243,937 - IAI47_20060 0.23 +0.6 243,937 - IAI47_20060 0.23 -1.7 243,937 - IAI47_20060 0.23 -0.4 243,937 - IAI47_20060 0.23 +0.8 244,031 + IAI47_20060 0.35 +0.3 244,032 - IAI47_20060 0.35 +2.4 244,032 - IAI47_20060 0.35 +0.3 244,040 + IAI47_20060 0.36 -0.6 244,040 + IAI47_20060 0.36 +0.6 244,066 - IAI47_20060 0.39 +0.1 244,066 - IAI47_20060 0.39 +0.9 244,073 + IAI47_20060 0.40 +0.2 244,073 + IAI47_20060 0.40 -1.8 244,073 + IAI47_20060 0.40 -1.5 244,074 - IAI47_20060 0.40 -0.4 244,074 - -0.2 244,074 - IAI47_20060 0.40 -0.0 244,077 + IAI47_20060 0.41 -0.6 244,077 + IAI47_20060 0.41 -0.2 244,077 + IAI47_20060 0.41 -1.1 244,078 - IAI47_20060 0.41 +1.1 244,078 - IAI47_20060 0.41 +0.6 244,078 - IAI47_20060 0.41 -0.7 244,122 + IAI47_20060 0.46 -0.1 244,122 + IAI47_20060 0.46 +0.3 244,122 + IAI47_20060 0.46 -1.1 244,122 + IAI47_20060 0.46 -2.4 244,123 - IAI47_20060 0.47 -1.1 244,123 - IAI47_20060 0.47 +0.1 244,123 - IAI47_20060 0.47 +1.2 244,123 - IAI47_20060 0.47 +1.5 244,123 - IAI47_20060 0.47 -0.8 244,123 - IAI47_20060 0.47 -0.6 244,123 - IAI47_20060 0.47 +0.1 244,125 + IAI47_20060 0.47 +0.2 244,125 + IAI47_20060 0.47 -0.0 244,125 + IAI47_20060 0.47 +2.6 244,125 + IAI47_20060 0.47 +0.1 244,126 - IAI47_20060 0.47 -2.0 244,127 + IAI47_20060 0.47 -1.6 244,127 + IAI47_20060 0.47 -0.2 244,127 + IAI47_20060 0.47 -1.1 244,128 - IAI47_20060 0.47 -0.4 244,128 - IAI47_20060 0.47 +0.5 244,128 - IAI47_20060 0.47 -2.2 244,128 - IAI47_20060 0.47 +0.6 244,128 - IAI47_20060 0.47 -0.5 244,169 + IAI47_20060 0.52 -1.4 244,170 - IAI47_20060 0.52 -1.6 244,239 + IAI47_20060 0.61 -2.0 244,239 + IAI47_20060 0.61 +1.3 244,277 + IAI47_20060 0.66 +0.9 244,277 + IAI47_20060 0.66 +1.8 244,278 - IAI47_20060 0.66 -0.3 244,278 - IAI47_20060 0.66 +2.4 244,278 - IAI47_20060 0.66 +0.1 244,278 - IAI47_20060 0.66 -0.4 244,416 + IAI47_20060 0.83 -0.4 244,417 - IAI47_20060 0.84 +1.4 244,433 + IAI47_20060 0.86 +0.3 244,434 - IAI47_20060 0.86 -0.1 244,434 - IAI47_20060 0.86 -1.5 244,434 - IAI47_20060 0.86 +1.7 244,546 - +0.1 244,546 - +0.7 244,546 - +0.4 244,636 - +0.4 244,636 - +1.2 244,636 - +3.9 244,636 - -1.3 244,636 - -0.9 244,668 - -0.9 244,729 + +0.9 244,766 + IAI47_20065 0.18 -1.3 244,767 - IAI47_20065 0.18 -0.7 244,831 + IAI47_20065 0.37 -2.0 244,831 + IAI47_20065 0.37 -1.2 244,831 + IAI47_20065 0.37 -0.6 244,832 - IAI47_20065 0.37 +0.7 244,832 - IAI47_20065 0.37 +0.3 244,913 + IAI47_20065 0.61 +0.8 244,913 + IAI47_20065 0.61 -0.8 244,915 - IAI47_20065 0.62 -0.4 245,043 + -0.4 245,121 + +2.3 245,128 + -1.5 245,129 - +0.7 245,132 + +0.3 245,132 + -0.7 245,133 - -0.2 245,133 - -0.1 245,133 - -0.6 245,133 - -1.8 245,239 + IAI47_20070 0.10 -1.4 245,239 + IAI47_20070 0.10 -0.7 245,240 - IAI47_20070 0.11 +0.1 245,243 + IAI47_20070 0.11 +0.6 245,244 - IAI47_20070 0.11 -2.4 245,276 + IAI47_20070 0.14 -0.2 245,330 + IAI47_20070 0.19 +0.7 245,331 - IAI47_20070 0.19 -1.1 245,331 - IAI47_20070 0.19 -0.4 245,345 + IAI47_20070 0.20 +0.6 245,345 + IAI47_20070 0.20 -1.9 245,345 + IAI47_20070 0.20 +1.8 245,346 - IAI47_20070 0.20 -0.6 245,346 - IAI47_20070 0.20 +0.3 245,367 - IAI47_20070 0.22 -0.2 245,468 - IAI47_20070 0.31 +0.3 245,468 - IAI47_20070 0.31 +0.2 245,468 - IAI47_20070 0.31 +0.2 245,506 - IAI47_20070 0.34 +0.4 245,506 - IAI47_20070 0.34 +0.9 245,528 + IAI47_20070 0.36 +0.2 245,528 + IAI47_20070 0.36 +1.4 245,547 + IAI47_20070 0.38 +0.5 245,548 - IAI47_20070 0.38 +1.2 245,608 + IAI47_20070 0.43 +0.8 245,636 + IAI47_20070 0.46 -0.4 245,637 - IAI47_20070 0.46 +0.8 245,637 - IAI47_20070 0.46 -0.8 245,637 - IAI47_20070 0.46 -0.5 245,676 - IAI47_20070 0.49 -0.7 245,789 + IAI47_20070 0.59 +0.4 245,789 + IAI47_20070 0.59 +0.4 245,789 + IAI47_20070 0.59 -0.4 245,789 + IAI47_20070 0.59 -0.3 245,790 - IAI47_20070 0.59 -1.2 245,790 - IAI47_20070 0.59 -0.3 245,790 - IAI47_20070 0.59 +0.4 245,852 + IAI47_20070 0.65 -0.4 245,853 - IAI47_20070 0.65 +0.5 245,853 - IAI47_20070 0.65 -0.3 245,853 - IAI47_20070 0.65 +1.4 245,853 - IAI47_20070 0.65 -1.0 245,853 - IAI47_20070 0.65 -0.9 245,853 - IAI47_20070 0.65 +1.3 245,898 - IAI47_20070 0.69 -0.8 245,985 + IAI47_20070 0.76 +0.6
Or see this region's nucleotide sequence