Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_19790 and IAI47_19795 are separated by 186 nucleotides IAI47_19795 and IAI47_19800 are separated by 12 nucleotides
IAI47_19790: IAI47_19790 - sigma-54-dependent Fis family transcriptional regulator, at 182,543 to 184,504
_19790
IAI47_19795: IAI47_19795 - sugar porter family MFS transporter, at 184,691 to 186,139
_19795
IAI47_19800: IAI47_19800 - Gfo/Idh/MocA family oxidoreductase, at 186,152 to 187,165
_19800
Position (kb)
184
185
186
187 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 183.702 kb on - strand, within IAI47_19790 at 183.702 kb on - strand, within IAI47_19790 at 183.721 kb on + strand, within IAI47_19790 at 183.722 kb on - strand, within IAI47_19790 at 183.775 kb on + strand, within IAI47_19790 at 183.775 kb on + strand, within IAI47_19790 at 183.776 kb on - strand, within IAI47_19790 at 183.776 kb on - strand, within IAI47_19790 at 183.776 kb on - strand, within IAI47_19790 at 183.791 kb on - strand, within IAI47_19790 at 183.857 kb on + strand, within IAI47_19790 at 183.857 kb on + strand, within IAI47_19790 at 183.858 kb on - strand, within IAI47_19790 at 183.858 kb on - strand, within IAI47_19790 at 183.956 kb on + strand, within IAI47_19790 at 183.956 kb on + strand, within IAI47_19790 at 183.957 kb on - strand, within IAI47_19790 at 183.957 kb on - strand, within IAI47_19790 at 183.957 kb on - strand, within IAI47_19790 at 183.957 kb on - strand, within IAI47_19790 at 183.957 kb on - strand, within IAI47_19790 at 183.957 kb on - strand, within IAI47_19790 at 183.976 kb on + strand, within IAI47_19790 at 183.976 kb on + strand, within IAI47_19790 at 183.976 kb on + strand, within IAI47_19790 at 183.977 kb on - strand, within IAI47_19790 at 183.980 kb on + strand, within IAI47_19790 at 183.980 kb on + strand, within IAI47_19790 at 183.980 kb on + strand, within IAI47_19790 at 184.050 kb on + strand, within IAI47_19790 at 184.050 kb on + strand, within IAI47_19790 at 184.051 kb on - strand, within IAI47_19790 at 184.051 kb on - strand, within IAI47_19790 at 184.051 kb on - strand, within IAI47_19790 at 184.051 kb on - strand, within IAI47_19790 at 184.090 kb on + strand, within IAI47_19790 at 184.090 kb on + strand, within IAI47_19790 at 184.090 kb on + strand, within IAI47_19790 at 184.090 kb on + strand, within IAI47_19790 at 184.091 kb on - strand, within IAI47_19790 at 184.091 kb on - strand, within IAI47_19790 at 184.101 kb on - strand, within IAI47_19790 at 184.118 kb on - strand, within IAI47_19790 at 184.160 kb on + strand, within IAI47_19790 at 184.160 kb on + strand, within IAI47_19790 at 184.160 kb on + strand, within IAI47_19790 at 184.160 kb on + strand, within IAI47_19790 at 184.161 kb on - strand, within IAI47_19790 at 184.161 kb on - strand, within IAI47_19790 at 184.183 kb on + strand, within IAI47_19790 at 184.183 kb on + strand, within IAI47_19790 at 184.184 kb on - strand, within IAI47_19790 at 184.201 kb on + strand, within IAI47_19790 at 184.201 kb on + strand, within IAI47_19790 at 184.201 kb on + strand, within IAI47_19790 at 184.202 kb on - strand, within IAI47_19790 at 184.203 kb on + strand, within IAI47_19790 at 184.203 kb on + strand, within IAI47_19790 at 184.204 kb on - strand, within IAI47_19790 at 184.204 kb on - strand, within IAI47_19790 at 184.299 kb on + strand, within IAI47_19790 at 184.299 kb on + strand, within IAI47_19790 at 184.300 kb on - strand, within IAI47_19790 at 184.300 kb on - strand, within IAI47_19790 at 184.300 kb on - strand, within IAI47_19790 at 184.303 kb on + strand, within IAI47_19790 at 184.303 kb on + strand, within IAI47_19790 at 184.303 kb on + strand, within IAI47_19790 at 184.304 kb on - strand, within IAI47_19790 at 184.322 kb on - strand at 184.326 kb on + strand at 184.327 kb on - strand at 184.327 kb on - strand at 184.327 kb on - strand at 184.357 kb on + strand at 184.358 kb on - strand at 184.358 kb on - strand at 184.358 kb on - strand at 184.415 kb on + strand at 184.415 kb on + strand at 184.415 kb on + strand at 184.416 kb on - strand at 184.485 kb on - strand at 184.485 kb on - strand at 184.485 kb on - strand at 184.485 kb on - strand at 184.487 kb on + strand at 184.524 kb on - strand at 184.524 kb on - strand at 184.548 kb on - strand at 184.682 kb on + strand at 184.683 kb on - strand at 184.683 kb on - strand at 184.691 kb on - strand at 184.764 kb on - strand at 184.776 kb on + strand at 184.777 kb on - strand at 184.818 kb on + strand at 184.818 kb on + strand at 184.906 kb on + strand, within IAI47_19795 at 184.907 kb on - strand, within IAI47_19795 at 184.926 kb on + strand, within IAI47_19795 at 184.926 kb on + strand, within IAI47_19795 at 184.926 kb on + strand, within IAI47_19795 at 184.926 kb on + strand, within IAI47_19795 at 184.927 kb on - strand, within IAI47_19795 at 185.048 kb on - strand, within IAI47_19795 at 185.057 kb on + strand, within IAI47_19795 at 185.144 kb on + strand, within IAI47_19795 at 185.145 kb on - strand, within IAI47_19795 at 185.145 kb on - strand, within IAI47_19795 at 185.145 kb on - strand, within IAI47_19795 at 185.145 kb on - strand, within IAI47_19795 at 185.145 kb on - strand, within IAI47_19795 at 185.146 kb on + strand, within IAI47_19795 at 185.156 kb on + strand, within IAI47_19795 at 185.244 kb on + strand, within IAI47_19795 at 185.244 kb on + strand, within IAI47_19795 at 185.244 kb on + strand, within IAI47_19795 at 185.244 kb on + strand, within IAI47_19795 at 185.244 kb on + strand, within IAI47_19795 at 185.244 kb on + strand, within IAI47_19795 at 185.368 kb on - strand, within IAI47_19795 at 185.368 kb on - strand, within IAI47_19795 at 185.447 kb on + strand, within IAI47_19795 at 185.447 kb on + strand, within IAI47_19795 at 185.448 kb on - strand, within IAI47_19795 at 185.448 kb on - strand, within IAI47_19795 at 185.448 kb on - strand, within IAI47_19795 at 185.448 kb on - strand, within IAI47_19795 at 185.448 kb on - strand, within IAI47_19795 at 185.448 kb on - strand, within IAI47_19795 at 185.448 kb on - strand, within IAI47_19795 at 185.448 kb on - strand, within IAI47_19795 at 185.448 kb on - strand, within IAI47_19795 at 185.449 kb on + strand, within IAI47_19795 at 185.449 kb on + strand, within IAI47_19795 at 185.449 kb on + strand, within IAI47_19795 at 185.450 kb on - strand, within IAI47_19795 at 185.476 kb on + strand, within IAI47_19795 at 185.514 kb on - strand, within IAI47_19795 at 185.514 kb on - strand, within IAI47_19795 at 185.532 kb on + strand, within IAI47_19795 at 185.533 kb on - strand, within IAI47_19795 at 185.623 kb on + strand, within IAI47_19795 at 185.624 kb on - strand, within IAI47_19795 at 185.686 kb on + strand, within IAI47_19795 at 185.686 kb on + strand, within IAI47_19795 at 185.686 kb on + strand, within IAI47_19795 at 185.687 kb on - strand, within IAI47_19795 at 185.687 kb on - strand, within IAI47_19795 at 185.711 kb on - strand, within IAI47_19795 at 185.711 kb on - strand, within IAI47_19795 at 185.711 kb on - strand, within IAI47_19795 at 185.766 kb on + strand, within IAI47_19795 at 185.767 kb on - strand, within IAI47_19795 at 185.767 kb on - strand, within IAI47_19795 at 185.821 kb on + strand, within IAI47_19795 at 185.867 kb on + strand, within IAI47_19795 at 185.867 kb on + strand, within IAI47_19795 at 185.867 kb on + strand, within IAI47_19795 at 185.868 kb on - strand, within IAI47_19795 at 185.868 kb on - strand, within IAI47_19795 at 185.876 kb on - strand, within IAI47_19795 at 185.881 kb on + strand, within IAI47_19795 at 185.882 kb on - strand, within IAI47_19795 at 185.961 kb on + strand, within IAI47_19795 at 185.962 kb on - strand, within IAI47_19795 at 185.962 kb on - strand, within IAI47_19795 at 185.962 kb on - strand, within IAI47_19795 at 185.962 kb on - strand, within IAI47_19795 at 186.137 kb on + strand at 186.138 kb on - strand at 186.140 kb on + strand at 186.140 kb on + strand at 186.168 kb on + strand at 186.169 kb on - strand at 186.320 kb on + strand, within IAI47_19800 at 186.320 kb on + strand, within IAI47_19800 at 186.323 kb on - strand, within IAI47_19800 at 186.451 kb on + strand, within IAI47_19800 at 186.452 kb on - strand, within IAI47_19800 at 186.452 kb on - strand, within IAI47_19800 at 186.517 kb on - strand, within IAI47_19800 at 186.529 kb on + strand, within IAI47_19800 at 186.604 kb on + strand, within IAI47_19800 at 186.605 kb on - strand, within IAI47_19800 at 186.702 kb on + strand, within IAI47_19800 at 186.740 kb on + strand, within IAI47_19800 at 186.775 kb on + strand, within IAI47_19800 at 186.781 kb on + strand, within IAI47_19800 at 186.796 kb on + strand, within IAI47_19800 at 186.797 kb on - strand, within IAI47_19800 at 186.812 kb on - strand, within IAI47_19800 at 186.812 kb on - strand, within IAI47_19800 at 186.832 kb on + strand, within IAI47_19800 at 186.838 kb on + strand, within IAI47_19800 at 186.838 kb on + strand, within IAI47_19800 at 186.839 kb on - strand, within IAI47_19800 at 186.839 kb on - strand, within IAI47_19800 at 186.839 kb on - strand, within IAI47_19800 at 186.840 kb on + strand, within IAI47_19800 at 186.840 kb on + strand, within IAI47_19800 at 186.841 kb on - strand, within IAI47_19800 at 186.866 kb on + strand, within IAI47_19800 at 186.866 kb on + strand, within IAI47_19800 at 186.866 kb on + strand, within IAI47_19800 at 186.867 kb on - strand, within IAI47_19800 at 186.867 kb on - strand, within IAI47_19800 at 186.872 kb on + strand, within IAI47_19800 at 186.872 kb on + strand, within IAI47_19800 at 186.950 kb on + strand, within IAI47_19800 at 186.951 kb on - strand, within IAI47_19800 at 186.976 kb on + strand, within IAI47_19800 at 186.977 kb on - strand, within IAI47_19800 at 187.013 kb on + strand, within IAI47_19800 at 187.013 kb on + strand, within IAI47_19800 at 187.013 kb on + strand, within IAI47_19800 at 187.013 kb on + strand, within IAI47_19800 at 187.013 kb on + strand, within IAI47_19800 at 187.013 kb on + strand, within IAI47_19800 at 187.014 kb on - strand, within IAI47_19800 at 187.014 kb on - strand, within IAI47_19800 at 187.014 kb on - strand, within IAI47_19800 at 187.014 kb on - strand, within IAI47_19800 at 187.014 kb on - strand, within IAI47_19800 at 187.014 kb on - strand, within IAI47_19800 at 187.014 kb on - strand, within IAI47_19800 at 187.128 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 183,702 - IAI47_19790 0.59 +0.9 183,702 - IAI47_19790 0.59 -0.6 183,721 + IAI47_19790 0.60 -0.6 183,722 - IAI47_19790 0.60 +1.6 183,775 + IAI47_19790 0.63 -1.0 183,775 + IAI47_19790 0.63 +0.3 183,776 - IAI47_19790 0.63 +0.3 183,776 - IAI47_19790 0.63 -0.4 183,776 - IAI47_19790 0.63 -1.1 183,791 - IAI47_19790 0.64 +2.2 183,857 + IAI47_19790 0.67 -1.1 183,857 + IAI47_19790 0.67 +1.4 183,858 - IAI47_19790 0.67 -2.0 183,858 - IAI47_19790 0.67 +0.6 183,956 + IAI47_19790 0.72 -0.1 183,956 + IAI47_19790 0.72 +0.0 183,957 - IAI47_19790 0.72 +0.0 183,957 - IAI47_19790 0.72 +0.2 183,957 - IAI47_19790 0.72 +1.6 183,957 - IAI47_19790 0.72 -2.4 183,957 - IAI47_19790 0.72 -0.5 183,957 - IAI47_19790 0.72 -0.4 183,976 + IAI47_19790 0.73 +0.0 183,976 + IAI47_19790 0.73 +0.4 183,976 + IAI47_19790 0.73 -1.4 183,977 - IAI47_19790 0.73 +1.2 183,980 + IAI47_19790 0.73 -0.1 183,980 + IAI47_19790 0.73 +0.1 183,980 + IAI47_19790 0.73 +2.0 184,050 + IAI47_19790 0.77 -0.2 184,050 + IAI47_19790 0.77 +0.5 184,051 - IAI47_19790 0.77 +0.8 184,051 - IAI47_19790 0.77 +1.4 184,051 - IAI47_19790 0.77 -2.3 184,051 - IAI47_19790 0.77 +2.0 184,090 + IAI47_19790 0.79 -1.4 184,090 + IAI47_19790 0.79 +0.4 184,090 + IAI47_19790 0.79 +0.9 184,090 + IAI47_19790 0.79 +3.0 184,091 - IAI47_19790 0.79 -0.3 184,091 - IAI47_19790 0.79 -0.4 184,101 - IAI47_19790 0.79 -0.5 184,118 - IAI47_19790 0.80 +0.4 184,160 + IAI47_19790 0.82 -0.2 184,160 + IAI47_19790 0.82 +3.0 184,160 + IAI47_19790 0.82 +0.5 184,160 + IAI47_19790 0.82 -1.1 184,161 - IAI47_19790 0.82 +1.2 184,161 - IAI47_19790 0.82 +0.9 184,183 + IAI47_19790 0.84 +3.2 184,183 + IAI47_19790 0.84 -1.0 184,184 - IAI47_19790 0.84 +0.4 184,201 + IAI47_19790 0.85 +0.1 184,201 + IAI47_19790 0.85 -0.6 184,201 + IAI47_19790 0.85 +0.4 184,202 - IAI47_19790 0.85 -0.1 184,203 + IAI47_19790 0.85 +0.0 184,203 + IAI47_19790 0.85 +1.0 184,204 - IAI47_19790 0.85 -0.0 184,204 - IAI47_19790 0.85 -0.9 184,299 + IAI47_19790 0.90 +1.1 184,299 + IAI47_19790 0.90 +0.1 184,300 - IAI47_19790 0.90 +0.5 184,300 - IAI47_19790 0.90 +1.2 184,300 - IAI47_19790 0.90 -0.3 184,303 + IAI47_19790 0.90 -1.2 184,303 + IAI47_19790 0.90 -2.2 184,303 + IAI47_19790 0.90 +0.6 184,304 - IAI47_19790 0.90 -0.4 184,322 - +0.6 184,326 + +0.4 184,327 - -1.6 184,327 - -0.8 184,327 - -0.3 184,357 + -1.2 184,358 - -1.0 184,358 - -0.6 184,358 - -1.0 184,415 + +0.2 184,415 + -1.5 184,415 + -0.7 184,416 - -0.7 184,485 - -1.1 184,485 - +1.0 184,485 - +0.2 184,485 - +1.6 184,487 + -0.9 184,524 - +0.8 184,524 - +0.8 184,548 - -0.0 184,682 + +0.2 184,683 - +0.4 184,683 - -0.6 184,691 - -1.2 184,764 - -1.2 184,776 + -0.8 184,777 - -1.6 184,818 + +0.6 184,818 + -0.2 184,906 + IAI47_19795 0.15 +1.2 184,907 - IAI47_19795 0.15 -1.0 184,926 + IAI47_19795 0.16 -1.1 184,926 + IAI47_19795 0.16 -0.8 184,926 + IAI47_19795 0.16 +0.0 184,926 + IAI47_19795 0.16 +0.4 184,927 - IAI47_19795 0.16 -0.0 185,048 - IAI47_19795 0.25 -0.1 185,057 + IAI47_19795 0.25 -0.3 185,144 + IAI47_19795 0.31 +1.4 185,145 - IAI47_19795 0.31 -1.9 185,145 - IAI47_19795 0.31 -1.4 185,145 - IAI47_19795 0.31 +0.4 185,145 - IAI47_19795 0.31 -0.9 185,145 - IAI47_19795 0.31 +5.2 185,146 + IAI47_19795 0.31 +1.6 185,156 + IAI47_19795 0.32 -0.9 185,244 + IAI47_19795 0.38 +2.4 185,244 + IAI47_19795 0.38 -0.6 185,244 + IAI47_19795 0.38 -1.4 185,244 + IAI47_19795 0.38 +0.8 185,244 + IAI47_19795 0.38 +0.4 185,244 + IAI47_19795 0.38 -0.2 185,368 - IAI47_19795 0.47 +0.9 185,368 - IAI47_19795 0.47 +0.4 185,447 + IAI47_19795 0.52 +0.0 185,447 + IAI47_19795 0.52 -2.3 185,448 - IAI47_19795 0.52 +0.9 185,448 - IAI47_19795 0.52 +0.3 185,448 - IAI47_19795 0.52 +0.8 185,448 - IAI47_19795 0.52 +1.2 185,448 - IAI47_19795 0.52 +0.9 185,448 - IAI47_19795 0.52 +0.8 185,448 - IAI47_19795 0.52 +0.9 185,448 - IAI47_19795 0.52 -0.6 185,448 - IAI47_19795 0.52 -1.4 185,449 + IAI47_19795 0.52 -2.1 185,449 + IAI47_19795 0.52 -1.5 185,449 + IAI47_19795 0.52 +1.4 185,450 - IAI47_19795 0.52 +0.5 185,476 + IAI47_19795 0.54 +0.0 185,514 - IAI47_19795 0.57 +0.8 185,514 - IAI47_19795 0.57 +0.4 185,532 + IAI47_19795 0.58 +0.7 185,533 - IAI47_19795 0.58 -1.2 185,623 + IAI47_19795 0.64 +0.0 185,624 - IAI47_19795 0.64 +0.4 185,686 + IAI47_19795 0.69 +3.6 185,686 + IAI47_19795 0.69 +1.4 185,686 + IAI47_19795 0.69 +0.9 185,687 - IAI47_19795 0.69 +0.6 185,687 - IAI47_19795 0.69 +1.6 185,711 - IAI47_19795 0.70 -1.4 185,711 - IAI47_19795 0.70 +0.5 185,711 - IAI47_19795 0.70 +1.8 185,766 + IAI47_19795 0.74 +0.8 185,767 - IAI47_19795 0.74 +1.2 185,767 - IAI47_19795 0.74 -0.4 185,821 + IAI47_19795 0.78 -0.2 185,867 + IAI47_19795 0.81 +1.4 185,867 + IAI47_19795 0.81 +3.0 185,867 + IAI47_19795 0.81 -0.1 185,868 - IAI47_19795 0.81 +0.5 185,868 - IAI47_19795 0.81 +1.3 185,876 - IAI47_19795 0.82 +0.5 185,881 + IAI47_19795 0.82 +0.5 185,882 - IAI47_19795 0.82 +0.8 185,961 + IAI47_19795 0.88 -0.5 185,962 - IAI47_19795 0.88 -0.4 185,962 - IAI47_19795 0.88 -0.1 185,962 - IAI47_19795 0.88 -0.1 185,962 - IAI47_19795 0.88 +1.2 186,137 + +0.8 186,138 - -0.2 186,140 + -1.6 186,140 + +1.5 186,168 + -1.8 186,169 - +1.4 186,320 + IAI47_19800 0.17 +1.0 186,320 + IAI47_19800 0.17 -0.9 186,323 - IAI47_19800 0.17 -0.0 186,451 + IAI47_19800 0.29 +1.0 186,452 - IAI47_19800 0.30 +0.1 186,452 - IAI47_19800 0.30 -0.3 186,517 - IAI47_19800 0.36 -0.4 186,529 + IAI47_19800 0.37 -0.5 186,604 + IAI47_19800 0.45 -0.1 186,605 - IAI47_19800 0.45 +0.8 186,702 + IAI47_19800 0.54 +1.9 186,740 + IAI47_19800 0.58 -0.6 186,775 + IAI47_19800 0.61 +0.2 186,781 + IAI47_19800 0.62 -0.0 186,796 + IAI47_19800 0.64 +1.2 186,797 - IAI47_19800 0.64 +0.6 186,812 - IAI47_19800 0.65 -0.2 186,812 - IAI47_19800 0.65 +0.4 186,832 + IAI47_19800 0.67 -0.2 186,838 + IAI47_19800 0.68 -1.6 186,838 + IAI47_19800 0.68 +1.8 186,839 - IAI47_19800 0.68 -0.2 186,839 - IAI47_19800 0.68 +0.6 186,839 - IAI47_19800 0.68 -0.1 186,840 + IAI47_19800 0.68 -0.4 186,840 + IAI47_19800 0.68 +0.6 186,841 - IAI47_19800 0.68 -1.0 186,866 + IAI47_19800 0.70 -0.9 186,866 + IAI47_19800 0.70 +0.5 186,866 + IAI47_19800 0.70 -3.1 186,867 - IAI47_19800 0.71 +0.0 186,867 - IAI47_19800 0.71 -1.7 186,872 + IAI47_19800 0.71 -1.0 186,872 + IAI47_19800 0.71 -1.3 186,950 + IAI47_19800 0.79 +1.1 186,951 - IAI47_19800 0.79 -1.4 186,976 + IAI47_19800 0.81 -1.6 186,977 - IAI47_19800 0.81 +1.1 187,013 + IAI47_19800 0.85 -0.8 187,013 + IAI47_19800 0.85 +1.8 187,013 + IAI47_19800 0.85 -1.8 187,013 + IAI47_19800 0.85 +0.2 187,013 + IAI47_19800 0.85 +1.0 187,013 + IAI47_19800 0.85 -0.4 187,014 - IAI47_19800 0.85 -0.6 187,014 - IAI47_19800 0.85 -0.1 187,014 - IAI47_19800 0.85 -0.5 187,014 - IAI47_19800 0.85 -0.4 187,014 - IAI47_19800 0.85 -2.0 187,014 - IAI47_19800 0.85 +0.1 187,014 - IAI47_19800 0.85 -0.4 187,128 + -1.6
Or see this region's nucleotide sequence