Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_11800 and IAI47_11805 are separated by 2 nucleotides IAI47_11805 and IAI47_11810 overlap by 1 nucleotides IAI47_11810 and IAI47_11815 are separated by 2 nucleotides
IAI47_11800: IAI47_11800 - ABC transporter permease, at 2,503,356 to 2,504,462
_11800
IAI47_11805: IAI47_11805 - sugar ABC transporter ATP-binding protein, at 2,504,465 to 2,505,979
_11805
IAI47_11810: IAI47_11810 - DUF2291 family protein, at 2,505,979 to 2,506,578
_11810
IAI47_11815: IAI47_11815 - D-ribose ABC transporter substrate-binding protein, at 2,506,581 to 2,507,522
_11815
Position (kb)
2504
2505
2506 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2503.718 kb on + strand, within IAI47_11800 at 2503.718 kb on + strand, within IAI47_11800 at 2503.719 kb on - strand, within IAI47_11800 at 2503.719 kb on - strand, within IAI47_11800 at 2503.719 kb on - strand, within IAI47_11800 at 2503.719 kb on - strand, within IAI47_11800 at 2503.719 kb on - strand, within IAI47_11800 at 2503.721 kb on - strand, within IAI47_11800 at 2503.721 kb on - strand, within IAI47_11800 at 2503.721 kb on - strand, within IAI47_11800 at 2503.721 kb on - strand, within IAI47_11800 at 2503.826 kb on + strand, within IAI47_11800 at 2503.827 kb on - strand, within IAI47_11800 at 2503.828 kb on + strand, within IAI47_11800 at 2503.828 kb on + strand, within IAI47_11800 at 2503.828 kb on + strand, within IAI47_11800 at 2503.828 kb on + strand, within IAI47_11800 at 2503.828 kb on + strand, within IAI47_11800 at 2503.829 kb on - strand, within IAI47_11800 at 2503.829 kb on - strand, within IAI47_11800 at 2503.829 kb on - strand, within IAI47_11800 at 2503.829 kb on - strand, within IAI47_11800 at 2503.829 kb on - strand, within IAI47_11800 at 2503.829 kb on - strand, within IAI47_11800 at 2503.870 kb on + strand, within IAI47_11800 at 2503.870 kb on + strand, within IAI47_11800 at 2503.870 kb on + strand, within IAI47_11800 at 2503.870 kb on + strand, within IAI47_11800 at 2503.871 kb on - strand, within IAI47_11800 at 2503.871 kb on - strand, within IAI47_11800 at 2503.871 kb on - strand, within IAI47_11800 at 2503.917 kb on - strand, within IAI47_11800 at 2503.920 kb on - strand, within IAI47_11800 at 2503.934 kb on + strand, within IAI47_11800 at 2503.934 kb on + strand, within IAI47_11800 at 2503.996 kb on + strand, within IAI47_11800 at 2503.996 kb on + strand, within IAI47_11800 at 2503.996 kb on + strand, within IAI47_11800 at 2503.996 kb on + strand, within IAI47_11800 at 2503.996 kb on + strand, within IAI47_11800 at 2503.996 kb on + strand, within IAI47_11800 at 2503.996 kb on + strand, within IAI47_11800 at 2503.996 kb on + strand, within IAI47_11800 at 2503.997 kb on - strand, within IAI47_11800 at 2503.997 kb on - strand, within IAI47_11800 at 2503.997 kb on - strand, within IAI47_11800 at 2503.997 kb on - strand, within IAI47_11800 at 2503.997 kb on - strand, within IAI47_11800 at 2503.997 kb on - strand, within IAI47_11800 at 2503.997 kb on - strand, within IAI47_11800 at 2503.997 kb on - strand, within IAI47_11800 at 2503.997 kb on - strand, within IAI47_11800 at 2503.997 kb on - strand, within IAI47_11800 at 2504.035 kb on + strand, within IAI47_11800 at 2504.036 kb on - strand, within IAI47_11800 at 2504.036 kb on - strand, within IAI47_11800 at 2504.036 kb on - strand, within IAI47_11800 at 2504.070 kb on - strand, within IAI47_11800 at 2504.231 kb on + strand, within IAI47_11800 at 2504.246 kb on + strand, within IAI47_11800 at 2504.247 kb on - strand, within IAI47_11800 at 2504.247 kb on - strand, within IAI47_11800 at 2504.369 kb on + strand at 2504.370 kb on - strand at 2504.370 kb on - strand at 2504.370 kb on - strand at 2504.411 kb on + strand at 2504.411 kb on + strand at 2504.411 kb on + strand at 2504.411 kb on + strand at 2504.412 kb on - strand at 2504.412 kb on - strand at 2504.412 kb on - strand at 2504.450 kb on + strand at 2504.776 kb on + strand, within IAI47_11805 at 2504.776 kb on + strand, within IAI47_11805 at 2504.776 kb on + strand, within IAI47_11805 at 2504.776 kb on + strand, within IAI47_11805 at 2504.776 kb on + strand, within IAI47_11805 at 2504.813 kb on - strand, within IAI47_11805 at 2504.905 kb on + strand, within IAI47_11805 at 2504.906 kb on - strand, within IAI47_11805 at 2505.004 kb on + strand, within IAI47_11805 at 2505.005 kb on - strand, within IAI47_11805 at 2505.017 kb on - strand, within IAI47_11805 at 2505.017 kb on - strand, within IAI47_11805 at 2505.017 kb on - strand, within IAI47_11805 at 2505.042 kb on + strand, within IAI47_11805 at 2505.043 kb on - strand, within IAI47_11805 at 2505.043 kb on - strand, within IAI47_11805 at 2505.043 kb on - strand, within IAI47_11805 at 2505.043 kb on - strand, within IAI47_11805 at 2505.105 kb on + strand, within IAI47_11805 at 2505.105 kb on + strand, within IAI47_11805 at 2505.105 kb on + strand, within IAI47_11805 at 2505.105 kb on + strand, within IAI47_11805 at 2505.106 kb on - strand, within IAI47_11805 at 2505.106 kb on - strand, within IAI47_11805 at 2505.106 kb on - strand, within IAI47_11805 at 2505.137 kb on - strand, within IAI47_11805 at 2505.160 kb on + strand, within IAI47_11805 at 2505.160 kb on + strand, within IAI47_11805 at 2505.161 kb on - strand, within IAI47_11805 at 2505.161 kb on - strand, within IAI47_11805 at 2505.341 kb on - strand, within IAI47_11805 at 2505.341 kb on - strand, within IAI47_11805 at 2505.385 kb on + strand, within IAI47_11805 at 2505.385 kb on + strand, within IAI47_11805 at 2505.385 kb on + strand, within IAI47_11805 at 2505.386 kb on - strand, within IAI47_11805 at 2505.387 kb on + strand, within IAI47_11805 at 2505.388 kb on - strand, within IAI47_11805 at 2505.388 kb on - strand, within IAI47_11805 at 2505.397 kb on + strand, within IAI47_11805 at 2505.578 kb on - strand, within IAI47_11805 at 2505.578 kb on - strand, within IAI47_11805 at 2505.691 kb on + strand, within IAI47_11805 at 2505.965 kb on - strand at 2505.965 kb on - strand at 2506.142 kb on + strand, within IAI47_11810 at 2506.142 kb on + strand, within IAI47_11810 at 2506.142 kb on + strand, within IAI47_11810 at 2506.143 kb on - strand, within IAI47_11810 at 2506.158 kb on + strand, within IAI47_11810 at 2506.158 kb on + strand, within IAI47_11810 at 2506.240 kb on - strand, within IAI47_11810 at 2506.245 kb on + strand, within IAI47_11810 at 2506.278 kb on + strand, within IAI47_11810 at 2506.278 kb on + strand, within IAI47_11810 at 2506.279 kb on - strand, within IAI47_11810 at 2506.407 kb on + strand, within IAI47_11810 at 2506.407 kb on + strand, within IAI47_11810 at 2506.458 kb on + strand, within IAI47_11810 at 2506.458 kb on + strand, within IAI47_11810 at 2506.458 kb on + strand, within IAI47_11810 at 2506.459 kb on - strand, within IAI47_11810 at 2506.459 kb on - strand, within IAI47_11810 at 2506.460 kb on + strand, within IAI47_11810 at 2506.460 kb on + strand, within IAI47_11810 at 2506.460 kb on + strand, within IAI47_11810 at 2506.461 kb on - strand, within IAI47_11810 at 2506.461 kb on - strand, within IAI47_11810 at 2506.461 kb on - strand, within IAI47_11810 at 2506.461 kb on - strand, within IAI47_11810 at 2506.461 kb on - strand, within IAI47_11810 at 2506.461 kb on - strand, within IAI47_11810 at 2506.500 kb on + strand, within IAI47_11810 at 2506.558 kb on + strand at 2506.559 kb on - strand at 2506.567 kb on + strand at 2506.568 kb on - strand at 2506.568 kb on - strand at 2506.568 kb on - strand at 2506.569 kb on + strand at 2506.569 kb on + strand at 2506.569 kb on + strand at 2506.569 kb on + strand at 2506.569 kb on + strand at 2506.569 kb on + strand at 2506.570 kb on - strand at 2506.570 kb on - strand at 2506.582 kb on + strand at 2506.582 kb on + strand at 2506.583 kb on - strand at 2506.583 kb on - strand at 2506.687 kb on + strand, within IAI47_11815 at 2506.687 kb on + strand, within IAI47_11815 at 2506.688 kb on - strand, within IAI47_11815 at 2506.690 kb on + strand, within IAI47_11815 at 2506.690 kb on + strand, within IAI47_11815 at 2506.691 kb on - strand, within IAI47_11815 at 2506.691 kb on - strand, within IAI47_11815 at 2506.691 kb on - strand, within IAI47_11815 at 2506.754 kb on + strand, within IAI47_11815 at 2506.754 kb on + strand, within IAI47_11815 at 2506.786 kb on + strand, within IAI47_11815
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 2,503,718 + IAI47_11800 0.33 -0.8 2,503,718 + IAI47_11800 0.33 -0.5 2,503,719 - IAI47_11800 0.33 -1.4 2,503,719 - IAI47_11800 0.33 -0.5 2,503,719 - IAI47_11800 0.33 +1.2 2,503,719 - IAI47_11800 0.33 -1.9 2,503,719 - IAI47_11800 0.33 -0.9 2,503,721 - IAI47_11800 0.33 +0.0 2,503,721 - IAI47_11800 0.33 -1.4 2,503,721 - IAI47_11800 0.33 -0.9 2,503,721 - IAI47_11800 0.33 -1.2 2,503,826 + IAI47_11800 0.42 +0.8 2,503,827 - IAI47_11800 0.43 +1.6 2,503,828 + IAI47_11800 0.43 +1.9 2,503,828 + IAI47_11800 0.43 +0.0 2,503,828 + IAI47_11800 0.43 -1.0 2,503,828 + IAI47_11800 0.43 -1.1 2,503,828 + IAI47_11800 0.43 +0.3 2,503,829 - IAI47_11800 0.43 -0.0 2,503,829 - IAI47_11800 0.43 +1.0 2,503,829 - IAI47_11800 0.43 +1.6 2,503,829 - IAI47_11800 0.43 -1.6 2,503,829 - IAI47_11800 0.43 +2.6 2,503,829 - IAI47_11800 0.43 +0.5 2,503,870 + IAI47_11800 0.46 +2.0 2,503,870 + IAI47_11800 0.46 -1.4 2,503,870 + IAI47_11800 0.46 +1.2 2,503,870 + IAI47_11800 0.46 +0.6 2,503,871 - IAI47_11800 0.47 +0.7 2,503,871 - IAI47_11800 0.47 +1.2 2,503,871 - IAI47_11800 0.47 -0.4 2,503,917 - IAI47_11800 0.51 +0.5 2,503,920 - IAI47_11800 0.51 -1.3 2,503,934 + IAI47_11800 0.52 -1.1 2,503,934 + IAI47_11800 0.52 +1.2 2,503,996 + IAI47_11800 0.58 +1.1 2,503,996 + IAI47_11800 0.58 +0.9 2,503,996 + IAI47_11800 0.58 -0.5 2,503,996 + IAI47_11800 0.58 +1.4 2,503,996 + IAI47_11800 0.58 -0.1 2,503,996 + IAI47_11800 0.58 +1.0 2,503,996 + IAI47_11800 0.58 +0.0 2,503,996 + IAI47_11800 0.58 -0.2 2,503,997 - IAI47_11800 0.58 -1.6 2,503,997 - IAI47_11800 0.58 +0.9 2,503,997 - IAI47_11800 0.58 +0.4 2,503,997 - IAI47_11800 0.58 -1.5 2,503,997 - IAI47_11800 0.58 -2.3 2,503,997 - IAI47_11800 0.58 -1.1 2,503,997 - IAI47_11800 0.58 +1.0 2,503,997 - IAI47_11800 0.58 +0.4 2,503,997 - IAI47_11800 0.58 +0.6 2,503,997 - IAI47_11800 0.58 -1.0 2,504,035 + IAI47_11800 0.61 -0.3 2,504,036 - IAI47_11800 0.61 +0.8 2,504,036 - IAI47_11800 0.61 -1.3 2,504,036 - IAI47_11800 0.61 +0.7 2,504,070 - IAI47_11800 0.64 -0.5 2,504,231 + IAI47_11800 0.79 -0.4 2,504,246 + IAI47_11800 0.80 +2.2 2,504,247 - IAI47_11800 0.80 +1.4 2,504,247 - IAI47_11800 0.80 -1.1 2,504,369 + +2.6 2,504,370 - -1.7 2,504,370 - -0.5 2,504,370 - -1.3 2,504,411 + +0.4 2,504,411 + +3.2 2,504,411 + -1.1 2,504,411 + -0.8 2,504,412 - +0.6 2,504,412 - -0.7 2,504,412 - +0.6 2,504,450 + +1.5 2,504,776 + IAI47_11805 0.21 +0.7 2,504,776 + IAI47_11805 0.21 -1.9 2,504,776 + IAI47_11805 0.21 -0.3 2,504,776 + IAI47_11805 0.21 -1.1 2,504,776 + IAI47_11805 0.21 +1.4 2,504,813 - IAI47_11805 0.23 +1.0 2,504,905 + IAI47_11805 0.29 +2.5 2,504,906 - IAI47_11805 0.29 -0.1 2,505,004 + IAI47_11805 0.36 -1.1 2,505,005 - IAI47_11805 0.36 -0.1 2,505,017 - IAI47_11805 0.36 +1.5 2,505,017 - IAI47_11805 0.36 -0.9 2,505,017 - IAI47_11805 0.36 -0.7 2,505,042 + IAI47_11805 0.38 +0.6 2,505,043 - IAI47_11805 0.38 -0.7 2,505,043 - IAI47_11805 0.38 +1.9 2,505,043 - IAI47_11805 0.38 +0.5 2,505,043 - IAI47_11805 0.38 -0.3 2,505,105 + IAI47_11805 0.42 -0.6 2,505,105 + IAI47_11805 0.42 -0.7 2,505,105 + IAI47_11805 0.42 -0.6 2,505,105 + IAI47_11805 0.42 -1.0 2,505,106 - IAI47_11805 0.42 -0.6 2,505,106 - IAI47_11805 0.42 +1.6 2,505,106 - IAI47_11805 0.42 +1.0 2,505,137 - IAI47_11805 0.44 +1.3 2,505,160 + IAI47_11805 0.46 +0.8 2,505,160 + IAI47_11805 0.46 +0.9 2,505,161 - IAI47_11805 0.46 +1.6 2,505,161 - IAI47_11805 0.46 -0.1 2,505,341 - IAI47_11805 0.58 +1.8 2,505,341 - IAI47_11805 0.58 -0.1 2,505,385 + IAI47_11805 0.61 -0.6 2,505,385 + IAI47_11805 0.61 +0.7 2,505,385 + IAI47_11805 0.61 +2.9 2,505,386 - IAI47_11805 0.61 -0.7 2,505,387 + IAI47_11805 0.61 +1.6 2,505,388 - IAI47_11805 0.61 -0.8 2,505,388 - IAI47_11805 0.61 +0.2 2,505,397 + IAI47_11805 0.62 +0.9 2,505,578 - IAI47_11805 0.73 +0.2 2,505,578 - IAI47_11805 0.73 -0.8 2,505,691 + IAI47_11805 0.81 +2.9 2,505,965 - +1.0 2,505,965 - -0.1 2,506,142 + IAI47_11810 0.27 +0.6 2,506,142 + IAI47_11810 0.27 -2.6 2,506,142 + IAI47_11810 0.27 -0.5 2,506,143 - IAI47_11810 0.27 -1.7 2,506,158 + IAI47_11810 0.30 +0.0 2,506,158 + IAI47_11810 0.30 +1.3 2,506,240 - IAI47_11810 0.43 -2.4 2,506,245 + IAI47_11810 0.44 +0.3 2,506,278 + IAI47_11810 0.50 -0.0 2,506,278 + IAI47_11810 0.50 -0.2 2,506,279 - IAI47_11810 0.50 -1.0 2,506,407 + IAI47_11810 0.71 +0.4 2,506,407 + IAI47_11810 0.71 -0.1 2,506,458 + IAI47_11810 0.80 +0.3 2,506,458 + IAI47_11810 0.80 +0.8 2,506,458 + IAI47_11810 0.80 -1.5 2,506,459 - IAI47_11810 0.80 +1.8 2,506,459 - IAI47_11810 0.80 +0.1 2,506,460 + IAI47_11810 0.80 -0.5 2,506,460 + IAI47_11810 0.80 +0.5 2,506,460 + IAI47_11810 0.80 -0.6 2,506,461 - IAI47_11810 0.80 +2.2 2,506,461 - IAI47_11810 0.80 +1.2 2,506,461 - IAI47_11810 0.80 -1.6 2,506,461 - IAI47_11810 0.80 +0.7 2,506,461 - IAI47_11810 0.80 -0.7 2,506,461 - IAI47_11810 0.80 +0.4 2,506,500 + IAI47_11810 0.87 -0.8 2,506,558 + +0.4 2,506,559 - +2.2 2,506,567 + -1.5 2,506,568 - +1.1 2,506,568 - +1.0 2,506,568 - +0.4 2,506,569 + +0.2 2,506,569 + +2.7 2,506,569 + -0.1 2,506,569 + +1.0 2,506,569 + +0.7 2,506,569 + +0.9 2,506,570 - +0.7 2,506,570 - +0.3 2,506,582 + +2.1 2,506,582 + -0.5 2,506,583 - -0.1 2,506,583 - +0.3 2,506,687 + IAI47_11815 0.11 -0.8 2,506,687 + IAI47_11815 0.11 -0.1 2,506,688 - IAI47_11815 0.11 +0.2 2,506,690 + IAI47_11815 0.12 -1.6 2,506,690 + IAI47_11815 0.12 -1.2 2,506,691 - IAI47_11815 0.12 +0.6 2,506,691 - IAI47_11815 0.12 +1.8 2,506,691 - IAI47_11815 0.12 +0.7 2,506,754 + IAI47_11815 0.18 -1.7 2,506,754 + IAI47_11815 0.18 +1.4 2,506,786 + IAI47_11815 0.22 +0.3
Or see this region's nucleotide sequence