Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_03615 and IAI47_03620 are separated by 87 nucleotides IAI47_03620 and IAI47_03625 are separated by 70 nucleotides IAI47_03625 and IAI47_03630 are separated by 75 nucleotides
IAI47_03615: IAI47_03615 - glutathione synthase, at 752,383 to 753,327
_03615
IAI47_03620: IAI47_03620 - 16S rRNA (uracil(1498)-N(3))-methyltransferase, at 753,415 to 754,146
_03620
IAI47_03625: IAI47_03625 - deoxyribonuclease I, at 754,217 to 754,921
_03625
IAI47_03630: IAI47_03630 - SprT family zinc-dependent metalloprotease, at 754,997 to 755,509
_03630
Position (kb) 753
754
755 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 752.458 kb on + strand at 752.458 kb on + strand at 752.458 kb on + strand at 752.458 kb on + strand at 752.459 kb on - strand at 752.459 kb on - strand at 752.459 kb on - strand at 752.482 kb on - strand, within IAI47_03615 at 752.482 kb on - strand, within IAI47_03615 at 752.482 kb on - strand, within IAI47_03615 at 752.496 kb on + strand, within IAI47_03615 at 752.497 kb on - strand, within IAI47_03615 at 752.587 kb on + strand, within IAI47_03615 at 752.587 kb on + strand, within IAI47_03615 at 752.667 kb on - strand, within IAI47_03615 at 752.667 kb on - strand, within IAI47_03615 at 752.729 kb on + strand, within IAI47_03615 at 752.729 kb on + strand, within IAI47_03615 at 752.729 kb on + strand, within IAI47_03615 at 752.729 kb on + strand, within IAI47_03615 at 752.729 kb on + strand, within IAI47_03615 at 752.730 kb on - strand, within IAI47_03615 at 752.730 kb on - strand, within IAI47_03615 at 752.794 kb on - strand, within IAI47_03615 at 752.794 kb on - strand, within IAI47_03615 at 752.794 kb on - strand, within IAI47_03615 at 752.821 kb on - strand, within IAI47_03615 at 752.835 kb on + strand, within IAI47_03615 at 752.835 kb on + strand, within IAI47_03615 at 752.835 kb on + strand, within IAI47_03615 at 752.836 kb on - strand, within IAI47_03615 at 752.836 kb on - strand, within IAI47_03615 at 752.836 kb on - strand, within IAI47_03615 at 752.859 kb on + strand, within IAI47_03615 at 752.859 kb on + strand, within IAI47_03615 at 752.859 kb on + strand, within IAI47_03615 at 752.859 kb on + strand, within IAI47_03615 at 752.860 kb on - strand, within IAI47_03615 at 752.925 kb on + strand, within IAI47_03615 at 752.948 kb on + strand, within IAI47_03615 at 752.949 kb on - strand, within IAI47_03615 at 752.949 kb on - strand, within IAI47_03615 at 752.949 kb on - strand, within IAI47_03615 at 752.949 kb on - strand, within IAI47_03615 at 752.986 kb on - strand, within IAI47_03615 at 753.029 kb on + strand, within IAI47_03615 at 753.029 kb on + strand, within IAI47_03615 at 753.029 kb on + strand, within IAI47_03615 at 753.030 kb on - strand, within IAI47_03615 at 753.030 kb on - strand, within IAI47_03615 at 753.038 kb on + strand, within IAI47_03615 at 753.038 kb on + strand, within IAI47_03615 at 753.038 kb on + strand, within IAI47_03615 at 753.039 kb on - strand, within IAI47_03615 at 753.039 kb on - strand, within IAI47_03615 at 753.039 kb on - strand, within IAI47_03615 at 753.219 kb on - strand, within IAI47_03615 at 753.219 kb on - strand, within IAI47_03615 at 753.279 kb on + strand at 753.283 kb on - strand at 753.297 kb on + strand at 753.297 kb on + strand at 753.297 kb on + strand at 753.298 kb on - strand at 753.298 kb on - strand at 753.298 kb on - strand at 753.298 kb on - strand at 753.299 kb on - strand at 753.321 kb on + strand at 753.321 kb on + strand at 753.321 kb on + strand at 753.322 kb on - strand at 753.328 kb on + strand at 753.328 kb on + strand at 753.328 kb on + strand at 753.328 kb on + strand at 753.328 kb on + strand at 753.329 kb on - strand at 753.329 kb on - strand at 753.329 kb on - strand at 753.329 kb on - strand at 753.329 kb on - strand at 753.331 kb on + strand at 753.331 kb on + strand at 753.331 kb on + strand at 753.331 kb on + strand at 753.331 kb on + strand at 753.332 kb on - strand at 753.332 kb on - strand at 753.332 kb on - strand at 753.332 kb on - strand at 753.409 kb on + strand at 753.410 kb on - strand at 753.416 kb on + strand at 753.417 kb on - strand at 753.450 kb on + strand at 753.481 kb on + strand at 753.481 kb on + strand at 753.481 kb on + strand at 753.481 kb on + strand at 753.482 kb on - strand at 753.482 kb on - strand at 753.482 kb on - strand at 753.482 kb on - strand at 753.482 kb on - strand at 753.482 kb on - strand at 753.484 kb on + strand at 753.485 kb on - strand at 753.485 kb on - strand at 753.511 kb on - strand, within IAI47_03620 at 753.646 kb on + strand, within IAI47_03620 at 753.646 kb on + strand, within IAI47_03620 at 753.714 kb on + strand, within IAI47_03620 at 753.714 kb on + strand, within IAI47_03620 at 753.714 kb on + strand, within IAI47_03620 at 753.715 kb on - strand, within IAI47_03620 at 753.715 kb on - strand, within IAI47_03620 at 753.715 kb on - strand, within IAI47_03620 at 753.715 kb on - strand, within IAI47_03620 at 753.715 kb on - strand, within IAI47_03620 at 753.797 kb on - strand, within IAI47_03620 at 753.797 kb on - strand, within IAI47_03620 at 753.825 kb on + strand, within IAI47_03620 at 753.825 kb on + strand, within IAI47_03620 at 753.825 kb on + strand, within IAI47_03620 at 753.825 kb on + strand, within IAI47_03620 at 753.825 kb on + strand, within IAI47_03620 at 753.825 kb on + strand, within IAI47_03620 at 753.825 kb on + strand, within IAI47_03620 at 753.825 kb on + strand, within IAI47_03620 at 753.825 kb on + strand, within IAI47_03620 at 753.825 kb on + strand, within IAI47_03620 at 753.825 kb on + strand, within IAI47_03620 at 753.826 kb on - strand, within IAI47_03620 at 753.826 kb on - strand, within IAI47_03620 at 753.826 kb on - strand, within IAI47_03620 at 753.826 kb on - strand, within IAI47_03620 at 753.826 kb on - strand, within IAI47_03620 at 753.826 kb on - strand, within IAI47_03620 at 753.826 kb on - strand, within IAI47_03620 at 753.826 kb on - strand, within IAI47_03620 at 753.826 kb on - strand, within IAI47_03620 at 753.826 kb on - strand, within IAI47_03620 at 753.826 kb on - strand, within IAI47_03620 at 753.831 kb on + strand, within IAI47_03620 at 753.831 kb on + strand, within IAI47_03620 at 753.901 kb on + strand, within IAI47_03620 at 753.913 kb on + strand, within IAI47_03620 at 753.913 kb on + strand, within IAI47_03620 at 753.913 kb on + strand, within IAI47_03620 at 753.913 kb on + strand, within IAI47_03620 at 753.913 kb on + strand, within IAI47_03620 at 753.913 kb on + strand, within IAI47_03620 at 753.913 kb on + strand, within IAI47_03620 at 753.913 kb on + strand, within IAI47_03620 at 753.913 kb on + strand, within IAI47_03620 at 753.913 kb on + strand, within IAI47_03620 at 753.914 kb on - strand, within IAI47_03620 at 753.914 kb on - strand, within IAI47_03620 at 753.914 kb on - strand, within IAI47_03620 at 753.914 kb on - strand, within IAI47_03620 at 753.914 kb on - strand, within IAI47_03620 at 753.914 kb on - strand, within IAI47_03620 at 753.955 kb on + strand, within IAI47_03620 at 753.961 kb on + strand, within IAI47_03620 at 753.962 kb on - strand, within IAI47_03620 at 753.962 kb on - strand, within IAI47_03620 at 754.008 kb on + strand, within IAI47_03620 at 754.009 kb on - strand, within IAI47_03620 at 754.009 kb on - strand, within IAI47_03620 at 754.009 kb on - strand, within IAI47_03620 at 754.009 kb on - strand, within IAI47_03620 at 754.063 kb on + strand, within IAI47_03620 at 754.063 kb on + strand, within IAI47_03620 at 754.063 kb on + strand, within IAI47_03620 at 754.064 kb on - strand, within IAI47_03620 at 754.064 kb on - strand, within IAI47_03620 at 754.064 kb on - strand, within IAI47_03620 at 754.064 kb on - strand, within IAI47_03620 at 754.127 kb on + strand at 754.127 kb on + strand at 754.127 kb on + strand at 754.127 kb on + strand at 754.127 kb on + strand at 754.127 kb on + strand at 754.128 kb on - strand at 754.128 kb on - strand at 754.138 kb on + strand at 754.138 kb on + strand at 754.138 kb on + strand at 754.138 kb on + strand at 754.139 kb on - strand at 754.139 kb on - strand at 754.139 kb on - strand at 754.146 kb on + strand at 754.147 kb on - strand at 754.147 kb on - strand at 754.199 kb on + strand at 754.199 kb on + strand at 754.199 kb on + strand at 754.199 kb on + strand at 754.199 kb on + strand at 754.199 kb on + strand at 754.199 kb on + strand at 754.199 kb on + strand at 754.199 kb on + strand at 754.199 kb on + strand at 754.199 kb on + strand at 754.199 kb on + strand at 754.200 kb on - strand at 754.200 kb on - strand at 754.200 kb on - strand at 754.200 kb on - strand at 754.200 kb on - strand at 754.200 kb on - strand at 754.204 kb on + strand at 754.205 kb on - strand at 754.205 kb on - strand at 754.205 kb on - strand at 754.205 kb on - strand at 754.242 kb on + strand at 754.242 kb on + strand at 754.242 kb on + strand at 754.242 kb on + strand at 754.243 kb on - strand at 754.243 kb on - strand at 754.243 kb on - strand at 754.243 kb on - strand at 754.243 kb on - strand at 754.311 kb on + strand, within IAI47_03625 at 754.311 kb on + strand, within IAI47_03625 at 754.311 kb on + strand, within IAI47_03625 at 754.312 kb on - strand, within IAI47_03625 at 754.368 kb on + strand, within IAI47_03625 at 754.369 kb on - strand, within IAI47_03625 at 754.383 kb on + strand, within IAI47_03625 at 754.383 kb on + strand, within IAI47_03625 at 754.383 kb on + strand, within IAI47_03625 at 754.383 kb on + strand, within IAI47_03625 at 754.384 kb on - strand, within IAI47_03625 at 754.384 kb on - strand, within IAI47_03625 at 754.389 kb on + strand, within IAI47_03625 at 754.389 kb on + strand, within IAI47_03625 at 754.389 kb on + strand, within IAI47_03625 at 754.389 kb on + strand, within IAI47_03625 at 754.389 kb on + strand, within IAI47_03625 at 754.389 kb on + strand, within IAI47_03625 at 754.389 kb on + strand, within IAI47_03625 at 754.389 kb on + strand, within IAI47_03625 at 754.390 kb on - strand, within IAI47_03625 at 754.390 kb on - strand, within IAI47_03625 at 754.390 kb on - strand, within IAI47_03625 at 754.449 kb on + strand, within IAI47_03625 at 754.473 kb on + strand, within IAI47_03625 at 754.504 kb on - strand, within IAI47_03625 at 754.678 kb on + strand, within IAI47_03625 at 754.678 kb on + strand, within IAI47_03625 at 754.678 kb on + strand, within IAI47_03625 at 754.678 kb on + strand, within IAI47_03625 at 754.679 kb on - strand, within IAI47_03625 at 754.679 kb on - strand, within IAI47_03625 at 754.679 kb on - strand, within IAI47_03625 at 754.714 kb on - strand, within IAI47_03625 at 754.765 kb on + strand, within IAI47_03625 at 754.776 kb on + strand, within IAI47_03625 at 754.776 kb on + strand, within IAI47_03625 at 754.837 kb on + strand, within IAI47_03625 at 754.837 kb on + strand, within IAI47_03625 at 754.837 kb on + strand, within IAI47_03625 at 754.838 kb on - strand, within IAI47_03625 at 754.848 kb on + strand, within IAI47_03625 at 754.848 kb on + strand, within IAI47_03625 at 754.848 kb on + strand, within IAI47_03625 at 754.849 kb on - strand, within IAI47_03625 at 754.849 kb on - strand, within IAI47_03625 at 754.849 kb on - strand, within IAI47_03625 at 754.849 kb on - strand, within IAI47_03625 at 754.849 kb on - strand, within IAI47_03625 at 754.849 kb on - strand, within IAI47_03625 at 754.849 kb on - strand, within IAI47_03625 at 754.852 kb on + strand at 754.852 kb on + strand at 754.852 kb on + strand at 754.852 kb on + strand at 754.852 kb on + strand at 754.852 kb on + strand at 754.852 kb on + strand at 754.853 kb on - strand at 754.853 kb on - strand at 754.853 kb on - strand at 754.853 kb on - strand at 754.853 kb on - strand at 754.907 kb on + strand at 754.907 kb on + strand at 754.907 kb on + strand at 754.908 kb on - strand at 754.921 kb on + strand at 754.921 kb on + strand at 754.921 kb on + strand at 754.921 kb on + strand at 754.921 kb on + strand at 754.922 kb on - strand at 754.922 kb on - strand at 754.922 kb on - strand at 754.922 kb on - strand at 754.922 kb on - strand at 754.922 kb on - strand at 754.922 kb on - strand at 754.933 kb on + strand at 754.933 kb on + strand at 754.933 kb on + strand at 754.933 kb on + strand at 754.933 kb on + strand at 754.934 kb on - strand at 754.934 kb on - strand at 754.937 kb on + strand at 754.937 kb on + strand at 754.937 kb on + strand at 754.937 kb on + strand at 754.938 kb on - strand at 754.938 kb on - strand at 754.940 kb on + strand at 754.940 kb on + strand at 754.942 kb on - strand at 754.961 kb on - strand at 754.975 kb on + strand at 754.976 kb on - strand at 754.976 kb on - strand at 754.976 kb on - strand at 754.976 kb on - strand at 755.008 kb on + strand at 755.009 kb on - strand at 755.052 kb on + strand, within IAI47_03630 at 755.052 kb on + strand, within IAI47_03630 at 755.053 kb on - strand, within IAI47_03630 at 755.053 kb on - strand, within IAI47_03630 at 755.053 kb on - strand, within IAI47_03630 at 755.053 kb on - strand, within IAI47_03630 at 755.124 kb on + strand, within IAI47_03630
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 752,458 + -1.1 752,458 + -1.7 752,458 + -0.2 752,458 + -1.1 752,459 - +0.9 752,459 - +0.1 752,459 - +0.7 752,482 - IAI47_03615 0.10 +0.0 752,482 - IAI47_03615 0.10 -0.3 752,482 - IAI47_03615 0.10 +0.7 752,496 + IAI47_03615 0.12 -0.3 752,497 - IAI47_03615 0.12 -1.1 752,587 + IAI47_03615 0.22 -1.9 752,587 + IAI47_03615 0.22 -0.8 752,667 - IAI47_03615 0.30 -1.4 752,667 - IAI47_03615 0.30 -2.3 752,729 + IAI47_03615 0.37 +1.0 752,729 + IAI47_03615 0.37 -1.3 752,729 + IAI47_03615 0.37 -0.2 752,729 + IAI47_03615 0.37 -1.1 752,729 + IAI47_03615 0.37 -0.3 752,730 - IAI47_03615 0.37 -0.0 752,730 - IAI47_03615 0.37 -1.0 752,794 - IAI47_03615 0.43 +0.1 752,794 - IAI47_03615 0.43 +0.1 752,794 - IAI47_03615 0.43 +0.7 752,821 - IAI47_03615 0.46 -1.4 752,835 + IAI47_03615 0.48 +0.1 752,835 + IAI47_03615 0.48 -1.3 752,835 + IAI47_03615 0.48 +0.0 752,836 - IAI47_03615 0.48 -0.5 752,836 - IAI47_03615 0.48 -1.9 752,836 - IAI47_03615 0.48 -0.4 752,859 + IAI47_03615 0.50 -1.0 752,859 + IAI47_03615 0.50 -1.7 752,859 + IAI47_03615 0.50 -0.4 752,859 + IAI47_03615 0.50 +0.7 752,860 - IAI47_03615 0.50 -0.6 752,925 + IAI47_03615 0.57 -0.4 752,948 + IAI47_03615 0.60 -2.0 752,949 - IAI47_03615 0.60 -0.1 752,949 - IAI47_03615 0.60 -0.9 752,949 - IAI47_03615 0.60 -2.3 752,949 - IAI47_03615 0.60 +1.6 752,986 - IAI47_03615 0.64 -2.6 753,029 + IAI47_03615 0.68 +1.4 753,029 + IAI47_03615 0.68 -3.2 753,029 + IAI47_03615 0.68 +1.3 753,030 - IAI47_03615 0.68 -1.1 753,030 - IAI47_03615 0.68 +0.3 753,038 + IAI47_03615 0.69 -0.9 753,038 + IAI47_03615 0.69 +1.0 753,038 + IAI47_03615 0.69 +0.4 753,039 - IAI47_03615 0.69 +1.0 753,039 - IAI47_03615 0.69 -1.0 753,039 - IAI47_03615 0.69 +0.3 753,219 - IAI47_03615 0.88 +0.2 753,219 - IAI47_03615 0.88 -0.9 753,279 + -1.5 753,283 - +1.9 753,297 + -1.6 753,297 + +1.2 753,297 + -1.5 753,298 - +1.8 753,298 - -1.8 753,298 - +1.1 753,298 - -1.5 753,299 - -0.8 753,321 + -1.6 753,321 + +0.3 753,321 + -0.1 753,322 - -0.7 753,328 + -0.6 753,328 + +0.0 753,328 + +0.7 753,328 + +1.0 753,328 + -1.3 753,329 - +0.4 753,329 - +0.4 753,329 - +2.7 753,329 - -1.2 753,329 - -0.9 753,331 + -1.9 753,331 + -0.7 753,331 + +0.4 753,331 + -2.4 753,331 + +0.4 753,332 - +0.1 753,332 - +1.2 753,332 - -0.3 753,332 - -0.3 753,409 + -1.1 753,410 - -1.1 753,416 + -0.1 753,417 - +0.0 753,450 + -1.2 753,481 + -0.0 753,481 + +2.3 753,481 + +1.0 753,481 + -0.8 753,482 - -0.5 753,482 - +1.3 753,482 - -0.0 753,482 - +1.1 753,482 - +0.4 753,482 - +1.6 753,484 + +0.6 753,485 - -0.8 753,485 - +1.9 753,511 - IAI47_03620 0.13 +1.7 753,646 + IAI47_03620 0.32 -0.2 753,646 + IAI47_03620 0.32 -0.1 753,714 + IAI47_03620 0.41 -1.3 753,714 + IAI47_03620 0.41 +0.3 753,714 + IAI47_03620 0.41 -0.4 753,715 - IAI47_03620 0.41 -0.3 753,715 - IAI47_03620 0.41 -1.0 753,715 - IAI47_03620 0.41 +2.7 753,715 - IAI47_03620 0.41 -0.7 753,715 - IAI47_03620 0.41 +0.6 753,797 - IAI47_03620 0.52 -1.4 753,797 - IAI47_03620 0.52 +0.7 753,825 + IAI47_03620 0.56 -2.4 753,825 + IAI47_03620 0.56 +0.3 753,825 + IAI47_03620 0.56 +1.4 753,825 + IAI47_03620 0.56 +2.5 753,825 + IAI47_03620 0.56 +0.8 753,825 + IAI47_03620 0.56 -0.3 753,825 + IAI47_03620 0.56 +3.1 753,825 + IAI47_03620 0.56 -0.5 753,825 + IAI47_03620 0.56 -0.2 753,825 + IAI47_03620 0.56 +0.3 753,825 + IAI47_03620 0.56 -1.6 753,826 - IAI47_03620 0.56 +0.7 753,826 - IAI47_03620 0.56 -0.3 753,826 - IAI47_03620 0.56 -0.1 753,826 - IAI47_03620 0.56 +0.1 753,826 - IAI47_03620 0.56 +0.9 753,826 - IAI47_03620 0.56 +0.6 753,826 - IAI47_03620 0.56 +1.0 753,826 - IAI47_03620 0.56 +0.4 753,826 - IAI47_03620 0.56 +1.3 753,826 - IAI47_03620 0.56 -0.6 753,826 - IAI47_03620 0.56 +2.1 753,831 + IAI47_03620 0.57 +0.1 753,831 + IAI47_03620 0.57 +1.4 753,901 + IAI47_03620 0.66 +0.7 753,913 + IAI47_03620 0.68 +1.0 753,913 + IAI47_03620 0.68 -0.4 753,913 + IAI47_03620 0.68 -2.5 753,913 + IAI47_03620 0.68 +1.1 753,913 + IAI47_03620 0.68 -0.3 753,913 + IAI47_03620 0.68 -0.1 753,913 + IAI47_03620 0.68 +0.2 753,913 + IAI47_03620 0.68 -0.1 753,913 + IAI47_03620 0.68 -0.7 753,913 + IAI47_03620 0.68 +2.9 753,914 - IAI47_03620 0.68 +0.6 753,914 - IAI47_03620 0.68 +0.3 753,914 - IAI47_03620 0.68 -1.8 753,914 - IAI47_03620 0.68 +0.2 753,914 - IAI47_03620 0.68 +0.4 753,914 - IAI47_03620 0.68 +3.1 753,955 + IAI47_03620 0.74 +1.2 753,961 + IAI47_03620 0.75 +0.2 753,962 - IAI47_03620 0.75 -0.5 753,962 - IAI47_03620 0.75 +0.6 754,008 + IAI47_03620 0.81 +0.9 754,009 - IAI47_03620 0.81 +0.2 754,009 - IAI47_03620 0.81 -0.3 754,009 - IAI47_03620 0.81 +0.7 754,009 - IAI47_03620 0.81 +0.7 754,063 + IAI47_03620 0.89 -0.0 754,063 + IAI47_03620 0.89 +0.0 754,063 + IAI47_03620 0.89 +0.4 754,064 - IAI47_03620 0.89 -0.8 754,064 - IAI47_03620 0.89 +0.4 754,064 - IAI47_03620 0.89 +1.2 754,064 - IAI47_03620 0.89 +0.4 754,127 + +1.6 754,127 + -1.9 754,127 + -2.0 754,127 + +0.3 754,127 + -0.1 754,127 + -2.7 754,128 - +1.0 754,128 - +0.3 754,138 + +0.4 754,138 + +0.3 754,138 + -1.3 754,138 + -1.3 754,139 - +0.0 754,139 - -1.0 754,139 - -1.0 754,146 + -0.3 754,147 - +1.8 754,147 - -0.7 754,199 + +1.1 754,199 + -2.4 754,199 + +0.1 754,199 + +2.0 754,199 + +2.5 754,199 + +1.1 754,199 + -0.7 754,199 + +2.5 754,199 + +0.2 754,199 + +1.3 754,199 + -1.5 754,199 + +0.6 754,200 - 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Or see this region's nucleotide sequence