Experiment: Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_01830 and IAI47_01835 are separated by 375 nucleotides IAI47_01835 and IAI47_01840 are separated by 49 nucleotides IAI47_01840 and IAI47_01845 are separated by 13 nucleotides
IAI47_01830: IAI47_01830 - phosphoenolpyruvate carboxykinase (ATP), at 401,108 to 402,727
_01830
IAI47_01835: IAI47_01835 - Hsp33 family molecular chaperone HslO, at 403,103 to 403,969
_01835
IAI47_01840: IAI47_01840 - ribosome-associated heat shock protein Hsp15, at 404,019 to 404,420
_01840
IAI47_01845: IAI47_01845 - GMP/IMP nucleotidase, at 404,434 to 405,081
_01845
Position (kb) 403
404 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 402.136 kb on + strand, within IAI47_01830 at 402.136 kb on + strand, within IAI47_01830 at 402.136 kb on + strand, within IAI47_01830 at 402.136 kb on + strand, within IAI47_01830 at 402.137 kb on - strand, within IAI47_01830 at 402.137 kb on - strand, within IAI47_01830 at 402.175 kb on + strand, within IAI47_01830 at 402.175 kb on + strand, within IAI47_01830 at 402.176 kb on - strand, within IAI47_01830 at 402.176 kb on - strand, within IAI47_01830 at 402.212 kb on - strand, within IAI47_01830 at 402.212 kb on - strand, within IAI47_01830 at 402.212 kb on - strand, within IAI47_01830 at 402.280 kb on + strand, within IAI47_01830 at 402.280 kb on + strand, within IAI47_01830 at 402.280 kb on + strand, within IAI47_01830 at 402.281 kb on - strand, within IAI47_01830 at 402.281 kb on - strand, within IAI47_01830 at 402.281 kb on - strand, within IAI47_01830 at 402.281 kb on - strand, within IAI47_01830 at 402.281 kb on - strand, within IAI47_01830 at 402.281 kb on - strand, within IAI47_01830 at 402.281 kb on - strand, within IAI47_01830 at 402.282 kb on - strand, within IAI47_01830 at 402.326 kb on + strand, within IAI47_01830 at 402.326 kb on + strand, within IAI47_01830 at 402.326 kb on + strand, within IAI47_01830 at 402.327 kb on - strand, within IAI47_01830 at 402.327 kb on - strand, within IAI47_01830 at 402.519 kb on + strand, within IAI47_01830 at 402.519 kb on + strand, within IAI47_01830 at 402.519 kb on + strand, within IAI47_01830 at 402.519 kb on + strand, within IAI47_01830 at 402.519 kb on + strand, within IAI47_01830 at 402.520 kb on - strand, within IAI47_01830 at 402.520 kb on - strand, within IAI47_01830 at 402.523 kb on + strand, within IAI47_01830 at 402.523 kb on + strand, within IAI47_01830 at 402.523 kb on + strand, within IAI47_01830 at 402.523 kb on + strand, within IAI47_01830 at 402.523 kb on + strand, within IAI47_01830 at 402.523 kb on + strand, within IAI47_01830 at 402.523 kb on + strand at 402.523 kb on + strand, within IAI47_01830 at 402.523 kb on + strand, within IAI47_01830 at 402.524 kb on - strand, within IAI47_01830 at 402.524 kb on - strand, within IAI47_01830 at 402.524 kb on - strand, within IAI47_01830 at 402.524 kb on - strand, within IAI47_01830 at 402.524 kb on - strand, within IAI47_01830 at 402.524 kb on - strand, within IAI47_01830 at 402.524 kb on - strand, within IAI47_01830 at 402.524 kb on - strand, within IAI47_01830 at 402.529 kb on + strand, within IAI47_01830 at 402.560 kb on + strand, within IAI47_01830 at 402.560 kb on + strand, within IAI47_01830 at 402.560 kb on + strand, within IAI47_01830 at 402.635 kb on + strand at 402.635 kb on + strand at 402.635 kb on + strand at 402.635 kb on + strand at 402.635 kb on + strand at 402.635 kb on + strand at 402.635 kb on + strand at 402.636 kb on - strand at 402.636 kb on - strand at 402.636 kb on - strand at 402.636 kb on - strand at 402.636 kb on - strand at 402.636 kb on - strand at 402.645 kb on + strand at 402.646 kb on - strand at 402.657 kb on + strand at 402.657 kb on + strand at 402.657 kb on + strand at 402.658 kb on - strand at 402.658 kb on - strand at 402.658 kb on - strand at 402.658 kb on - strand at 402.658 kb on - strand at 402.674 kb on - strand at 402.740 kb on + strand at 402.740 kb on + strand at 402.740 kb on + strand at 402.740 kb on + strand at 402.741 kb on - strand at 402.770 kb on + strand at 402.770 kb on + strand at 402.770 kb on + strand at 402.770 kb on + strand at 402.770 kb on + strand at 402.770 kb on + strand at 402.770 kb on + strand at 402.770 kb on + strand at 402.770 kb on + strand at 402.770 kb on + strand at 402.770 kb on + strand at 402.771 kb on - strand at 402.771 kb on - strand at 402.771 kb on - strand at 402.771 kb on - strand at 402.771 kb on - strand at 402.771 kb on - strand at 402.771 kb on - strand at 402.771 kb on - strand at 402.920 kb on + strand at 402.921 kb on - strand at 402.921 kb on - strand at 402.921 kb on - strand at 402.921 kb on - strand at 402.921 kb on - strand at 402.955 kb on + strand at 402.956 kb on - strand at 402.985 kb on - strand at 402.985 kb on - strand at 403.028 kb on - strand at 403.052 kb on + strand at 403.145 kb on - strand at 403.154 kb on - strand at 403.173 kb on + strand at 403.173 kb on + strand at 403.173 kb on + strand at 403.173 kb on + strand at 403.173 kb on + strand at 403.173 kb on + strand at 403.174 kb on - strand at 403.174 kb on - strand at 403.174 kb on - strand at 403.188 kb on + strand at 403.188 kb on + strand at 403.189 kb on - strand at 403.189 kb on - strand at 403.189 kb on - strand at 403.299 kb on + strand, within IAI47_01835 at 403.299 kb on + strand, within IAI47_01835 at 403.299 kb on + strand, within IAI47_01835 at 403.300 kb on - strand, within IAI47_01835 at 403.300 kb on - strand, within IAI47_01835 at 403.300 kb on - strand, within IAI47_01835 at 403.321 kb on - strand, within IAI47_01835 at 403.321 kb on - strand, within IAI47_01835 at 403.344 kb on + strand, within IAI47_01835 at 403.344 kb on + strand, within IAI47_01835 at 403.344 kb on + strand, within IAI47_01835 at 403.344 kb on + strand, within IAI47_01835 at 403.345 kb on - strand, within IAI47_01835 at 403.505 kb on - strand, within IAI47_01835 at 403.542 kb on + strand, within IAI47_01835 at 403.542 kb on + strand, within IAI47_01835 at 403.542 kb on + strand, within IAI47_01835 at 403.543 kb on - strand, within IAI47_01835 at 403.543 kb on - strand, within IAI47_01835 at 403.621 kb on + strand, within IAI47_01835 at 403.628 kb on + strand, within IAI47_01835 at 403.629 kb on - strand, within IAI47_01835 at 403.635 kb on + strand, within IAI47_01835 at 403.635 kb on + strand, within IAI47_01835 at 403.636 kb on - strand, within IAI47_01835 at 403.691 kb on - strand, within IAI47_01835 at 403.698 kb on - strand, within IAI47_01835 at 403.770 kb on - strand, within IAI47_01835 at 403.941 kb on + strand at 403.941 kb on + strand at 403.942 kb on - strand at 403.942 kb on - strand at 403.942 kb on - strand at 403.969 kb on + strand at 403.970 kb on - strand at 403.970 kb on - strand at 404.034 kb on + strand at 404.039 kb on + strand at 404.039 kb on + strand at 404.040 kb on - strand at 404.096 kb on + strand, within IAI47_01840 at 404.096 kb on + strand, within IAI47_01840 at 404.096 kb on + strand, within IAI47_01840 at 404.096 kb on + strand, within IAI47_01840 at 404.096 kb on + strand, within IAI47_01840 at 404.097 kb on - strand, within IAI47_01840 at 404.147 kb on + strand, within IAI47_01840 at 404.167 kb on + strand, within IAI47_01840 at 404.167 kb on + strand, within IAI47_01840 at 404.308 kb on + strand, within IAI47_01840 at 404.308 kb on + strand, within IAI47_01840 at 404.308 kb on + strand, within IAI47_01840 at 404.309 kb on - strand, within IAI47_01840 at 404.309 kb on - strand, within IAI47_01840 at 404.309 kb on - strand, within IAI47_01840 at 404.359 kb on + strand, within IAI47_01840 at 404.359 kb on + strand, within IAI47_01840 at 404.360 kb on - strand, within IAI47_01840 at 404.360 kb on - strand, within IAI47_01840 at 404.450 kb on + strand at 404.518 kb on + strand, within IAI47_01845 at 404.519 kb on - strand, within IAI47_01845 at 404.519 kb on - strand, within IAI47_01845 at 404.519 kb on - strand, within IAI47_01845 at 404.519 kb on - strand, within IAI47_01845 at 404.539 kb on + strand, within IAI47_01845 at 404.539 kb on + strand, within IAI47_01845 at 404.578 kb on - strand, within IAI47_01845 at 404.617 kb on + strand, within IAI47_01845 at 404.617 kb on + strand, within IAI47_01845 at 404.618 kb on - strand, within IAI47_01845 at 404.629 kb on + strand, within IAI47_01845 at 404.630 kb on - strand, within IAI47_01845 at 404.630 kb on - strand, within IAI47_01845 at 404.661 kb on + strand, within IAI47_01845 at 404.661 kb on + strand, within IAI47_01845 at 404.662 kb on - strand, within IAI47_01845 at 404.674 kb on + strand, within IAI47_01845 at 404.674 kb on + strand, within IAI47_01845 at 404.677 kb on + strand, within IAI47_01845 at 404.678 kb on - strand, within IAI47_01845 at 404.678 kb on - strand, within IAI47_01845 at 404.678 kb on - strand, within IAI47_01845 at 404.695 kb on + strand, within IAI47_01845 at 404.696 kb on - strand, within IAI47_01845 at 404.729 kb on + strand, within IAI47_01845 at 404.729 kb on + strand, within IAI47_01845 at 404.729 kb on + strand, within IAI47_01845 at 404.730 kb on - strand, within IAI47_01845 at 404.730 kb on - strand, within IAI47_01845 at 404.730 kb on - strand, within IAI47_01845 at 404.730 kb on - strand, within IAI47_01845 at 404.730 kb on - strand, within IAI47_01845 at 404.730 kb on - strand, within IAI47_01845 at 404.730 kb on - strand, within IAI47_01845 at 404.799 kb on + strand, within IAI47_01845 at 404.800 kb on - strand, within IAI47_01845 at 404.800 kb on - strand, within IAI47_01845 at 404.818 kb on + strand, within IAI47_01845 at 404.818 kb on + strand, within IAI47_01845 at 404.819 kb on - strand, within IAI47_01845 at 404.819 kb on - strand, within IAI47_01845 at 404.819 kb on - strand, within IAI47_01845 at 404.819 kb on - strand, within IAI47_01845 at 404.850 kb on + strand, within IAI47_01845 at 404.876 kb on + strand, within IAI47_01845 at 404.888 kb on + strand, within IAI47_01845 at 404.888 kb on + strand, within IAI47_01845 at 404.889 kb on - strand, within IAI47_01845 at 404.889 kb on - strand, within IAI47_01845 at 404.889 kb on - strand, within IAI47_01845 at 404.896 kb on + strand, within IAI47_01845 at 404.896 kb on + strand, within IAI47_01845 at 404.897 kb on - strand, within IAI47_01845 at 404.897 kb on - strand, within IAI47_01845 at 404.902 kb on - strand, within IAI47_01845 at 404.902 kb on - strand, within IAI47_01845 at 404.918 kb on + strand, within IAI47_01845
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH3.9 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 402,136 + IAI47_01830 0.63 +0.9 402,136 + IAI47_01830 0.63 +1.0 402,136 + IAI47_01830 0.63 -0.1 402,136 + IAI47_01830 0.63 +0.0 402,137 - IAI47_01830 0.64 +2.4 402,137 - IAI47_01830 0.64 +0.9 402,175 + IAI47_01830 0.66 -0.5 402,175 + IAI47_01830 0.66 -0.9 402,176 - IAI47_01830 0.66 -2.7 402,176 - IAI47_01830 0.66 +0.2 402,212 - IAI47_01830 0.68 +2.6 402,212 - IAI47_01830 0.68 +0.1 402,212 - IAI47_01830 0.68 -0.3 402,280 + IAI47_01830 0.72 +0.5 402,280 + IAI47_01830 0.72 +1.0 402,280 + IAI47_01830 0.72 +0.4 402,281 - IAI47_01830 0.72 +0.7 402,281 - IAI47_01830 0.72 -0.3 402,281 - IAI47_01830 0.72 -1.3 402,281 - IAI47_01830 0.72 +0.2 402,281 - IAI47_01830 0.72 +0.3 402,281 - IAI47_01830 0.72 -0.4 402,281 - IAI47_01830 0.72 +0.5 402,282 - IAI47_01830 0.72 -0.5 402,326 + IAI47_01830 0.75 +0.7 402,326 + IAI47_01830 0.75 -0.1 402,326 + IAI47_01830 0.75 +1.2 402,327 - IAI47_01830 0.75 +0.4 402,327 - IAI47_01830 0.75 -1.1 402,519 + IAI47_01830 0.87 -0.1 402,519 + IAI47_01830 0.87 +1.3 402,519 + IAI47_01830 0.87 +0.9 402,519 + IAI47_01830 0.87 -1.0 402,519 + IAI47_01830 0.87 +0.7 402,520 - IAI47_01830 0.87 -2.6 402,520 - IAI47_01830 0.87 -1.3 402,523 + IAI47_01830 0.87 +0.8 402,523 + IAI47_01830 0.87 -0.8 402,523 + IAI47_01830 0.87 -0.5 402,523 + IAI47_01830 0.87 -1.2 402,523 + IAI47_01830 0.87 -1.4 402,523 + IAI47_01830 0.87 -0.4 402,523 + -0.1 402,523 + IAI47_01830 0.87 +0.1 402,523 + IAI47_01830 0.87 +2.3 402,524 - IAI47_01830 0.87 -0.3 402,524 - IAI47_01830 0.87 -1.8 402,524 - IAI47_01830 0.87 +2.7 402,524 - IAI47_01830 0.87 -1.2 402,524 - IAI47_01830 0.87 -1.0 402,524 - IAI47_01830 0.87 +1.3 402,524 - IAI47_01830 0.87 +0.6 402,524 - IAI47_01830 0.87 -1.1 402,529 + IAI47_01830 0.88 +0.3 402,560 + IAI47_01830 0.90 -0.1 402,560 + IAI47_01830 0.90 +2.2 402,560 + IAI47_01830 0.90 -0.6 402,635 + -1.3 402,635 + -0.4 402,635 + +0.0 402,635 + -0.0 402,635 + -1.7 402,635 + -0.8 402,635 + -0.7 402,636 - -2.5 402,636 - +1.5 402,636 - -1.1 402,636 - -1.2 402,636 - +0.4 402,636 - -2.1 402,645 + -1.6 402,646 - -0.6 402,657 + +0.5 402,657 + -2.1 402,657 + +1.5 402,658 - +0.2 402,658 - -0.3 402,658 - +1.4 402,658 - +0.9 402,658 - -0.4 402,674 - +0.5 402,740 + +0.2 402,740 + -1.1 402,740 + -0.2 402,740 + -1.4 402,741 - +0.3 402,770 + -0.5 402,770 + +0.0 402,770 + -1.5 402,770 + -0.3 402,770 + -0.0 402,770 + +0.7 402,770 + -1.6 402,770 + -0.9 402,770 + +2.9 402,770 + -1.3 402,770 + +0.3 402,771 - -1.0 402,771 - +1.5 402,771 - +1.3 402,771 - +0.8 402,771 - -2.5 402,771 - +0.6 402,771 - -0.7 402,771 - +0.2 402,920 + +2.4 402,921 - -0.4 402,921 - +0.9 402,921 - +0.0 402,921 - -0.7 402,921 - -1.4 402,955 + -0.1 402,956 - -0.6 402,985 - +0.9 402,985 - +1.2 403,028 - +0.5 403,052 + +1.9 403,145 - +1.4 403,154 - -1.5 403,173 + -0.5 403,173 + +1.4 403,173 + -0.8 403,173 + +0.0 403,173 + -2.1 403,173 + -0.3 403,174 - +0.5 403,174 - -0.3 403,174 - +2.4 403,188 + -0.3 403,188 + +0.8 403,189 - +0.0 403,189 - -1.5 403,189 - -0.6 403,299 + IAI47_01835 0.23 -0.5 403,299 + IAI47_01835 0.23 +0.0 403,299 + IAI47_01835 0.23 -1.1 403,300 - IAI47_01835 0.23 -0.7 403,300 - IAI47_01835 0.23 -0.3 403,300 - IAI47_01835 0.23 -0.0 403,321 - IAI47_01835 0.25 -1.7 403,321 - IAI47_01835 0.25 -0.9 403,344 + IAI47_01835 0.28 -1.7 403,344 + IAI47_01835 0.28 -0.0 403,344 + IAI47_01835 0.28 -1.4 403,344 + IAI47_01835 0.28 -1.8 403,345 - IAI47_01835 0.28 -0.1 403,505 - IAI47_01835 0.46 -0.0 403,542 + IAI47_01835 0.51 -0.4 403,542 + IAI47_01835 0.51 -0.8 403,542 + IAI47_01835 0.51 +1.0 403,543 - IAI47_01835 0.51 -0.6 403,543 - IAI47_01835 0.51 -0.7 403,621 + IAI47_01835 0.60 -0.7 403,628 + IAI47_01835 0.61 -0.7 403,629 - IAI47_01835 0.61 -2.3 403,635 + IAI47_01835 0.61 +0.3 403,635 + IAI47_01835 0.61 -0.2 403,636 - IAI47_01835 0.61 -0.6 403,691 - IAI47_01835 0.68 +0.4 403,698 - IAI47_01835 0.69 +1.3 403,770 - IAI47_01835 0.77 -0.6 403,941 + +1.3 403,941 + -0.4 403,942 - -2.3 403,942 - -0.1 403,942 - -0.5 403,969 + +1.0 403,970 - +0.5 403,970 - +0.3 404,034 + -0.2 404,039 + -1.0 404,039 + +0.1 404,040 - -1.0 404,096 + IAI47_01840 0.19 +0.4 404,096 + IAI47_01840 0.19 +0.3 404,096 + IAI47_01840 0.19 +0.8 404,096 + IAI47_01840 0.19 -0.6 404,096 + IAI47_01840 0.19 -1.8 404,097 - IAI47_01840 0.19 +1.0 404,147 + IAI47_01840 0.32 +0.6 404,167 + IAI47_01840 0.37 -0.3 404,167 + IAI47_01840 0.37 -1.1 404,308 + IAI47_01840 0.72 -0.8 404,308 + IAI47_01840 0.72 -1.2 404,308 + IAI47_01840 0.72 +0.3 404,309 - IAI47_01840 0.72 +0.6 404,309 - IAI47_01840 0.72 +0.2 404,309 - IAI47_01840 0.72 +0.9 404,359 + IAI47_01840 0.85 -1.6 404,359 + IAI47_01840 0.85 -0.8 404,360 - IAI47_01840 0.85 +1.1 404,360 - IAI47_01840 0.85 +0.6 404,450 + +0.4 404,518 + IAI47_01845 0.13 +0.8 404,519 - IAI47_01845 0.13 -0.4 404,519 - IAI47_01845 0.13 +1.8 404,519 - IAI47_01845 0.13 -0.3 404,519 - IAI47_01845 0.13 +0.0 404,539 + IAI47_01845 0.16 -0.3 404,539 + IAI47_01845 0.16 +2.2 404,578 - IAI47_01845 0.22 -0.1 404,617 + IAI47_01845 0.28 -0.1 404,617 + IAI47_01845 0.28 +0.6 404,618 - IAI47_01845 0.28 +2.6 404,629 + IAI47_01845 0.30 -0.3 404,630 - IAI47_01845 0.30 -1.4 404,630 - IAI47_01845 0.30 -1.8 404,661 + IAI47_01845 0.35 -0.6 404,661 + IAI47_01845 0.35 -0.2 404,662 - IAI47_01845 0.35 -0.3 404,674 + IAI47_01845 0.37 +0.5 404,674 + IAI47_01845 0.37 -0.6 404,677 + IAI47_01845 0.38 -0.3 404,678 - IAI47_01845 0.38 -0.6 404,678 - IAI47_01845 0.38 -1.3 404,678 - IAI47_01845 0.38 +0.3 404,695 + IAI47_01845 0.40 -1.7 404,696 - IAI47_01845 0.40 +0.3 404,729 + IAI47_01845 0.46 -1.9 404,729 + IAI47_01845 0.46 +0.5 404,729 + IAI47_01845 0.46 -0.3 404,730 - IAI47_01845 0.46 -1.4 404,730 - IAI47_01845 0.46 +0.6 404,730 - IAI47_01845 0.46 -0.5 404,730 - IAI47_01845 0.46 +2.0 404,730 - IAI47_01845 0.46 -0.0 404,730 - IAI47_01845 0.46 -1.5 404,730 - IAI47_01845 0.46 -0.6 404,799 + IAI47_01845 0.56 +0.7 404,800 - IAI47_01845 0.56 +0.3 404,800 - IAI47_01845 0.56 -0.3 404,818 + IAI47_01845 0.59 -0.7 404,818 + IAI47_01845 0.59 +0.5 404,819 - IAI47_01845 0.59 +1.3 404,819 - IAI47_01845 0.59 -0.4 404,819 - IAI47_01845 0.59 -0.6 404,819 - IAI47_01845 0.59 -0.4 404,850 + IAI47_01845 0.64 -1.0 404,876 + IAI47_01845 0.68 +2.3 404,888 + IAI47_01845 0.70 -0.5 404,888 + IAI47_01845 0.70 -2.1 404,889 - IAI47_01845 0.70 +0.6 404,889 - IAI47_01845 0.70 -0.2 404,889 - IAI47_01845 0.70 +1.6 404,896 + IAI47_01845 0.71 +0.0 404,896 + IAI47_01845 0.71 +0.5 404,897 - IAI47_01845 0.71 +1.9 404,897 - IAI47_01845 0.71 -1.8 404,902 - IAI47_01845 0.72 +1.2 404,902 - IAI47_01845 0.72 +1.5 404,918 + IAI47_01845 0.75 -1.8
Or see this region's nucleotide sequence