Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20165 and IAI47_20170 are separated by 24 nucleotides IAI47_20170 and IAI47_20175 overlap by 4 nucleotides IAI47_20175 and IAI47_20180 overlap by 4 nucleotides
IAI47_20165: IAI47_20165 - ABC transporter substrate-binding protein, at 265,277 to 266,332
_20165
IAI47_20170: IAI47_20170 - ABC transporter permease, at 266,357 to 267,172
_20170
IAI47_20175: IAI47_20175 - ABC transporter ATP-binding protein, at 267,169 to 267,954
_20175
IAI47_20180: IAI47_20180 - urea carboxylase-associated family protein, at 267,951 to 268,679
_20180
Position (kb) 266
267
268 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 265.382 kb on + strand at 265.383 kb on - strand, within IAI47_20165 at 265.383 kb on - strand, within IAI47_20165 at 265.431 kb on + strand, within IAI47_20165 at 265.432 kb on + strand, within IAI47_20165 at 265.448 kb on + strand, within IAI47_20165 at 265.448 kb on + strand, within IAI47_20165 at 265.449 kb on - strand, within IAI47_20165 at 265.449 kb on - strand, within IAI47_20165 at 265.449 kb on - strand, within IAI47_20165 at 265.449 kb on - strand, within IAI47_20165 at 265.456 kb on + strand, within IAI47_20165 at 265.457 kb on - strand, within IAI47_20165 at 265.457 kb on - strand, within IAI47_20165 at 265.465 kb on + strand, within IAI47_20165 at 265.466 kb on - strand, within IAI47_20165 at 265.466 kb on - strand, within IAI47_20165 at 265.484 kb on + strand, within IAI47_20165 at 265.484 kb on + strand, within IAI47_20165 at 265.484 kb on + strand, within IAI47_20165 at 265.485 kb on - strand, within IAI47_20165 at 265.485 kb on - strand, within IAI47_20165 at 265.487 kb on - strand, within IAI47_20165 at 265.498 kb on + strand, within IAI47_20165 at 265.499 kb on - strand, within IAI47_20165 at 265.499 kb on - strand, within IAI47_20165 at 265.505 kb on + strand, within IAI47_20165 at 265.607 kb on + strand, within IAI47_20165 at 265.608 kb on - strand, within IAI47_20165 at 265.648 kb on + strand, within IAI47_20165 at 265.648 kb on + strand, within IAI47_20165 at 265.649 kb on - strand, within IAI47_20165 at 265.682 kb on + strand, within IAI47_20165 at 265.682 kb on + strand, within IAI47_20165 at 265.683 kb on - strand, within IAI47_20165 at 265.683 kb on - strand, within IAI47_20165 at 265.683 kb on - strand, within IAI47_20165 at 265.709 kb on + strand, within IAI47_20165 at 265.709 kb on + strand, within IAI47_20165 at 265.710 kb on - strand, within IAI47_20165 at 265.710 kb on - strand, within IAI47_20165 at 265.710 kb on - strand, within IAI47_20165 at 265.786 kb on + strand, within IAI47_20165 at 265.787 kb on - strand, within IAI47_20165 at 265.787 kb on - strand, within IAI47_20165 at 265.918 kb on + strand, within IAI47_20165 at 265.918 kb on + strand, within IAI47_20165 at 265.918 kb on + strand, within IAI47_20165 at 265.926 kb on + strand, within IAI47_20165 at 265.926 kb on + strand, within IAI47_20165 at 265.927 kb on - strand, within IAI47_20165 at 265.927 kb on - strand, within IAI47_20165 at 266.020 kb on + strand, within IAI47_20165 at 266.020 kb on + strand, within IAI47_20165 at 266.021 kb on - strand, within IAI47_20165 at 266.021 kb on - strand, within IAI47_20165 at 266.056 kb on + strand, within IAI47_20165 at 266.072 kb on + strand, within IAI47_20165 at 266.189 kb on - strand, within IAI47_20165 at 266.197 kb on - strand, within IAI47_20165 at 266.197 kb on - strand, within IAI47_20165 at 266.266 kb on + strand at 266.266 kb on + strand at 266.300 kb on + strand at 266.300 kb on + strand at 266.300 kb on + strand at 266.301 kb on - strand at 266.301 kb on - strand at 266.330 kb on + strand at 266.330 kb on + strand at 266.331 kb on - strand at 266.331 kb on - strand at 266.331 kb on - strand at 266.331 kb on - strand at 266.412 kb on + strand at 266.413 kb on - strand at 266.419 kb on + strand at 266.419 kb on + strand at 266.419 kb on + strand at 266.419 kb on + strand at 266.419 kb on + strand at 266.419 kb on + strand at 266.419 kb on + strand at 266.419 kb on + strand at 266.420 kb on - strand at 266.420 kb on - strand at 266.421 kb on + strand at 266.421 kb on + strand at 266.422 kb on - strand at 266.422 kb on - strand at 266.422 kb on - strand at 266.427 kb on + strand at 266.427 kb on + strand at 266.428 kb on - strand at 266.443 kb on + strand, within IAI47_20170 at 266.520 kb on + strand, within IAI47_20170 at 266.520 kb on + strand, within IAI47_20170 at 266.520 kb on + strand, within IAI47_20170 at 266.521 kb on - strand, within IAI47_20170 at 266.561 kb on + strand, within IAI47_20170 at 266.562 kb on - strand, within IAI47_20170 at 266.562 kb on - strand, within IAI47_20170 at 266.578 kb on + strand, within IAI47_20170 at 266.578 kb on + strand, within IAI47_20170 at 266.578 kb on + strand, within IAI47_20170 at 266.578 kb on + strand, within IAI47_20170 at 266.578 kb on + strand, within IAI47_20170 at 266.579 kb on - strand, within IAI47_20170 at 266.579 kb on - strand, within IAI47_20170 at 266.579 kb on - strand, within IAI47_20170 at 266.579 kb on - strand, within IAI47_20170 at 266.579 kb on - strand, within IAI47_20170 at 266.579 kb on - strand, within IAI47_20170 at 266.587 kb on + strand, within IAI47_20170 at 266.588 kb on - strand, within IAI47_20170 at 266.592 kb on + strand, within IAI47_20170 at 266.592 kb on + strand, within IAI47_20170 at 266.593 kb on - strand, within IAI47_20170 at 266.593 kb on - strand, within IAI47_20170 at 266.601 kb on + strand, within IAI47_20170 at 266.778 kb on + strand, within IAI47_20170 at 266.778 kb on + strand, within IAI47_20170 at 266.778 kb on + strand, within IAI47_20170 at 266.778 kb on + strand, within IAI47_20170 at 266.778 kb on + strand, within IAI47_20170 at 266.779 kb on - strand, within IAI47_20170 at 266.800 kb on + strand, within IAI47_20170 at 266.800 kb on + strand, within IAI47_20170 at 266.800 kb on + strand, within IAI47_20170 at 266.800 kb on + strand, within IAI47_20170 at 266.801 kb on - strand, within IAI47_20170 at 266.801 kb on - strand, within IAI47_20170 at 266.863 kb on + strand, within IAI47_20170 at 266.928 kb on + strand, within IAI47_20170 at 266.929 kb on - strand, within IAI47_20170 at 266.929 kb on - strand, within IAI47_20170 at 267.047 kb on + strand, within IAI47_20170 at 267.047 kb on + strand, within IAI47_20170 at 267.048 kb on - strand, within IAI47_20170 at 267.048 kb on - strand, within IAI47_20170 at 267.048 kb on - strand, within IAI47_20170 at 267.048 kb on - strand, within IAI47_20170 at 267.050 kb on - strand, within IAI47_20170 at 267.067 kb on + strand, within IAI47_20170 at 267.068 kb on - strand, within IAI47_20170 at 267.074 kb on + strand, within IAI47_20170 at 267.074 kb on + strand, within IAI47_20170 at 267.074 kb on + strand, within IAI47_20170 at 267.075 kb on - strand, within IAI47_20170 at 267.178 kb on - strand at 267.192 kb on + strand at 267.292 kb on - strand, within IAI47_20175 at 267.305 kb on + strand, within IAI47_20175 at 267.413 kb on - strand, within IAI47_20175 at 267.574 kb on - strand, within IAI47_20175 at 267.577 kb on + strand, within IAI47_20175 at 267.578 kb on - strand, within IAI47_20175 at 267.578 kb on - strand, within IAI47_20175 at 267.578 kb on - strand, within IAI47_20175 at 267.702 kb on + strand, within IAI47_20175 at 267.702 kb on + strand, within IAI47_20175 at 267.702 kb on + strand, within IAI47_20175 at 267.702 kb on + strand, within IAI47_20175 at 267.703 kb on - strand, within IAI47_20175 at 267.703 kb on - strand, within IAI47_20175 at 267.703 kb on - strand, within IAI47_20175 at 267.703 kb on - strand, within IAI47_20175 at 267.753 kb on + strand, within IAI47_20175 at 267.753 kb on + strand, within IAI47_20175 at 267.754 kb on - strand, within IAI47_20175 at 267.754 kb on - strand, within IAI47_20175 at 267.754 kb on - strand, within IAI47_20175 at 267.865 kb on + strand, within IAI47_20175 at 267.865 kb on + strand, within IAI47_20175 at 267.865 kb on + strand, within IAI47_20175 at 267.865 kb on + strand, within IAI47_20175 at 267.865 kb on + strand, within IAI47_20175 at 267.865 kb on + strand, within IAI47_20175 at 267.866 kb on - strand, within IAI47_20175 at 267.866 kb on - strand, within IAI47_20175 at 267.866 kb on - strand, within IAI47_20175 at 267.928 kb on + strand at 267.928 kb on + strand at 267.929 kb on - strand at 267.929 kb on - strand at 267.929 kb on - strand at 267.929 kb on - strand at 267.929 kb on - strand at 268.013 kb on + strand at 268.014 kb on - strand at 268.014 kb on - strand at 268.014 kb on - strand at 268.014 kb on - strand at 268.014 kb on - strand at 268.014 kb on - strand at 268.031 kb on + strand, within IAI47_20180 at 268.032 kb on - strand, within IAI47_20180
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 265,382 + +0.1 265,383 - IAI47_20165 0.10 +0.7 265,383 - IAI47_20165 0.10 +1.4 265,431 + IAI47_20165 0.15 +0.1 265,432 + IAI47_20165 0.15 -1.5 265,448 + IAI47_20165 0.16 -0.7 265,448 + IAI47_20165 0.16 -0.4 265,449 - IAI47_20165 0.16 +0.9 265,449 - IAI47_20165 0.16 +1.5 265,449 - IAI47_20165 0.16 -1.5 265,449 - IAI47_20165 0.16 +0.2 265,456 + IAI47_20165 0.17 +0.4 265,457 - IAI47_20165 0.17 -1.7 265,457 - IAI47_20165 0.17 +0.7 265,465 + IAI47_20165 0.18 +0.3 265,466 - IAI47_20165 0.18 -0.0 265,466 - IAI47_20165 0.18 -1.4 265,484 + IAI47_20165 0.20 +0.9 265,484 + IAI47_20165 0.20 -0.5 265,484 + IAI47_20165 0.20 -0.8 265,485 - IAI47_20165 0.20 -0.3 265,485 - IAI47_20165 0.20 -2.5 265,487 - IAI47_20165 0.20 -1.6 265,498 + IAI47_20165 0.21 +0.6 265,499 - IAI47_20165 0.21 -1.2 265,499 - IAI47_20165 0.21 +0.1 265,505 + IAI47_20165 0.22 +1.3 265,607 + IAI47_20165 0.31 +1.2 265,608 - IAI47_20165 0.31 +1.1 265,648 + IAI47_20165 0.35 -2.5 265,648 + IAI47_20165 0.35 +1.1 265,649 - IAI47_20165 0.35 +0.7 265,682 + IAI47_20165 0.38 -0.5 265,682 + IAI47_20165 0.38 +1.7 265,683 - IAI47_20165 0.38 -2.1 265,683 - IAI47_20165 0.38 -0.8 265,683 - IAI47_20165 0.38 +0.4 265,709 + IAI47_20165 0.41 -0.3 265,709 + IAI47_20165 0.41 +1.8 265,710 - IAI47_20165 0.41 -0.3 265,710 - IAI47_20165 0.41 -0.9 265,710 - IAI47_20165 0.41 -0.6 265,786 + IAI47_20165 0.48 +1.3 265,787 - IAI47_20165 0.48 -0.8 265,787 - IAI47_20165 0.48 -0.2 265,918 + IAI47_20165 0.61 +1.7 265,918 + IAI47_20165 0.61 +0.2 265,918 + IAI47_20165 0.61 +1.2 265,926 + IAI47_20165 0.61 +2.0 265,926 + IAI47_20165 0.61 +1.0 265,927 - IAI47_20165 0.62 -0.4 265,927 - IAI47_20165 0.62 -1.0 266,020 + IAI47_20165 0.70 -0.4 266,020 + IAI47_20165 0.70 -1.2 266,021 - IAI47_20165 0.70 +0.3 266,021 - IAI47_20165 0.70 +0.8 266,056 + IAI47_20165 0.74 -1.5 266,072 + IAI47_20165 0.75 +0.3 266,189 - IAI47_20165 0.86 +2.0 266,197 - IAI47_20165 0.87 +2.7 266,197 - IAI47_20165 0.87 +1.1 266,266 + -0.0 266,266 + +0.0 266,300 + +1.5 266,300 + -0.2 266,300 + +0.2 266,301 - +0.9 266,301 - -0.5 266,330 + -1.0 266,330 + -1.0 266,331 - -1.2 266,331 - -1.7 266,331 - -0.5 266,331 - +0.5 266,412 + -2.1 266,413 - +0.3 266,419 + +1.3 266,419 + -0.9 266,419 + +1.8 266,419 + +0.0 266,419 + -0.3 266,419 + -0.9 266,419 + -0.6 266,419 + +2.1 266,420 - -0.7 266,420 - +0.0 266,421 + -0.1 266,421 + +0.1 266,422 - +1.7 266,422 - +0.9 266,422 - +1.1 266,427 + +0.7 266,427 + -0.5 266,428 - +0.6 266,443 + IAI47_20170 0.11 -1.0 266,520 + IAI47_20170 0.20 +1.8 266,520 + IAI47_20170 0.20 +0.8 266,520 + IAI47_20170 0.20 +1.5 266,521 - IAI47_20170 0.20 +0.9 266,561 + IAI47_20170 0.25 +0.8 266,562 - IAI47_20170 0.25 -0.0 266,562 - IAI47_20170 0.25 -1.4 266,578 + IAI47_20170 0.27 +0.1 266,578 + IAI47_20170 0.27 -0.6 266,578 + IAI47_20170 0.27 +0.5 266,578 + IAI47_20170 0.27 +0.4 266,578 + IAI47_20170 0.27 +0.9 266,579 - IAI47_20170 0.27 +0.7 266,579 - IAI47_20170 0.27 -0.3 266,579 - IAI47_20170 0.27 +0.8 266,579 - IAI47_20170 0.27 -1.0 266,579 - IAI47_20170 0.27 +0.1 266,579 - IAI47_20170 0.27 -0.6 266,587 + IAI47_20170 0.28 +1.0 266,588 - IAI47_20170 0.28 +0.7 266,592 + IAI47_20170 0.29 +0.8 266,592 + IAI47_20170 0.29 +0.5 266,593 - IAI47_20170 0.29 +1.3 266,593 - IAI47_20170 0.29 +1.0 266,601 + IAI47_20170 0.30 -0.2 266,778 + IAI47_20170 0.52 -0.7 266,778 + IAI47_20170 0.52 -2.2 266,778 + IAI47_20170 0.52 +0.6 266,778 + IAI47_20170 0.52 -0.0 266,778 + IAI47_20170 0.52 +0.6 266,779 - IAI47_20170 0.52 +1.3 266,800 + IAI47_20170 0.54 -0.0 266,800 + IAI47_20170 0.54 +1.7 266,800 + IAI47_20170 0.54 +1.6 266,800 + IAI47_20170 0.54 -0.9 266,801 - IAI47_20170 0.54 +2.2 266,801 - IAI47_20170 0.54 +0.5 266,863 + IAI47_20170 0.62 -0.5 266,928 + IAI47_20170 0.70 +0.6 266,929 - IAI47_20170 0.70 +0.5 266,929 - IAI47_20170 0.70 +0.1 267,047 + IAI47_20170 0.85 +2.0 267,047 + IAI47_20170 0.85 -1.0 267,048 - IAI47_20170 0.85 +0.8 267,048 - IAI47_20170 0.85 +1.1 267,048 - IAI47_20170 0.85 -1.3 267,048 - IAI47_20170 0.85 +2.0 267,050 - IAI47_20170 0.85 -0.0 267,067 + IAI47_20170 0.87 -1.0 267,068 - IAI47_20170 0.87 +0.4 267,074 + IAI47_20170 0.88 +0.4 267,074 + IAI47_20170 0.88 +1.4 267,074 + IAI47_20170 0.88 +1.5 267,075 - IAI47_20170 0.88 -0.9 267,178 - -0.2 267,192 + +0.2 267,292 - IAI47_20175 0.16 -0.4 267,305 + IAI47_20175 0.17 +1.9 267,413 - IAI47_20175 0.31 -1.3 267,574 - IAI47_20175 0.52 -0.3 267,577 + IAI47_20175 0.52 -0.6 267,578 - IAI47_20175 0.52 +0.6 267,578 - IAI47_20175 0.52 +0.7 267,578 - IAI47_20175 0.52 -0.5 267,702 + IAI47_20175 0.68 +0.1 267,702 + IAI47_20175 0.68 +0.2 267,702 + IAI47_20175 0.68 +0.6 267,702 + IAI47_20175 0.68 +0.4 267,703 - IAI47_20175 0.68 +1.0 267,703 - IAI47_20175 0.68 +1.2 267,703 - IAI47_20175 0.68 -0.2 267,703 - IAI47_20175 0.68 +1.0 267,753 + IAI47_20175 0.74 -1.3 267,753 + IAI47_20175 0.74 +1.1 267,754 - IAI47_20175 0.74 +0.2 267,754 - IAI47_20175 0.74 -0.5 267,754 - IAI47_20175 0.74 +0.5 267,865 + IAI47_20175 0.89 -1.1 267,865 + IAI47_20175 0.89 +1.1 267,865 + IAI47_20175 0.89 -0.0 267,865 + IAI47_20175 0.89 +1.5 267,865 + IAI47_20175 0.89 +0.3 267,865 + IAI47_20175 0.89 -0.4 267,866 - IAI47_20175 0.89 -0.2 267,866 - IAI47_20175 0.89 -1.1 267,866 - IAI47_20175 0.89 +2.0 267,928 + -0.2 267,928 + -0.6 267,929 - -0.4 267,929 - +0.7 267,929 - -0.4 267,929 - +1.3 267,929 - +0.9 268,013 + -1.2 268,014 - -0.1 268,014 - +3.3 268,014 - +0.6 268,014 - +0.8 268,014 - +0.8 268,014 - +0.1 268,031 + IAI47_20180 0.11 +2.0 268,032 - IAI47_20180 0.11 +0.3
Or see this region's nucleotide sequence