Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_02315 and IAI47_02320 overlap by 4 nucleotides IAI47_02320 and IAI47_02325 overlap by 34 nucleotides IAI47_02325 and IAI47_02330 are separated by 491 nucleotides
IAI47_02315: IAI47_02315 - shikimate dehydrogenase, at 478,425 to 479,243
_02315
IAI47_02320: IAI47_02320 - DUF1488 domain-containing protein, at 479,240 to 479,497
_02320
IAI47_02325: IAI47_02325 - gamma carbonic anhydrase family protein, at 479,464 to 480,018
_02325
IAI47_02330: IAI47_02330 - 16S ribosomal RNA, at 480,510 to 482,056
_02330
Position (kb) 479
480
481 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 479.222 kb on + strand at 479.222 kb on + strand at 479.223 kb on - strand at 479.223 kb on - strand at 479.253 kb on + strand at 479.254 kb on - strand at 479.254 kb on - strand at 479.276 kb on + strand, within IAI47_02320 at 479.276 kb on + strand, within IAI47_02320 at 479.276 kb on + strand, within IAI47_02320 at 479.276 kb on + strand, within IAI47_02320 at 479.276 kb on + strand, within IAI47_02320 at 479.276 kb on + strand, within IAI47_02320 at 479.276 kb on + strand, within IAI47_02320 at 479.276 kb on + strand, within IAI47_02320 at 479.277 kb on - strand, within IAI47_02320 at 479.277 kb on - strand, within IAI47_02320 at 479.277 kb on - strand, within IAI47_02320 at 479.277 kb on - strand, within IAI47_02320 at 479.277 kb on - strand, within IAI47_02320 at 479.277 kb on - strand, within IAI47_02320 at 479.277 kb on - strand, within IAI47_02320 at 479.277 kb on - strand, within IAI47_02320 at 479.277 kb on - strand, within IAI47_02320 at 479.361 kb on + strand, within IAI47_02320 at 479.440 kb on + strand, within IAI47_02320 at 479.481 kb on + strand at 479.481 kb on + strand at 479.495 kb on + strand at 479.495 kb on + strand at 479.495 kb on + strand at 479.495 kb on + strand at 479.495 kb on + strand at 479.495 kb on + strand at 479.496 kb on - strand at 479.496 kb on - strand at 479.496 kb on - strand at 479.496 kb on - strand at 479.496 kb on - strand at 479.516 kb on + strand at 479.516 kb on + strand at 479.516 kb on + strand at 479.516 kb on + strand at 479.516 kb on + strand at 479.516 kb on + strand at 479.516 kb on + strand at 479.516 kb on + strand at 479.516 kb on + strand at 479.516 kb on + strand at 479.516 kb on + strand at 479.516 kb on + strand at 479.516 kb on + strand at 479.517 kb on - strand at 479.517 kb on - strand at 479.517 kb on - strand at 479.517 kb on - strand at 479.517 kb on - strand at 479.517 kb on - strand at 479.517 kb on - strand at 479.517 kb on - strand at 479.517 kb on - strand at 479.517 kb on - strand at 479.517 kb on - strand at 479.517 kb on - strand at 479.517 kb on - strand at 479.517 kb on - strand at 479.517 kb on - strand at 479.517 kb on - strand at 479.532 kb on - strand, within IAI47_02325 at 479.532 kb on - strand, within IAI47_02325 at 479.536 kb on + strand, within IAI47_02325 at 479.537 kb on - strand, within IAI47_02325 at 479.537 kb on - strand, within IAI47_02325 at 479.559 kb on + strand, within IAI47_02325 at 479.560 kb on - strand, within IAI47_02325 at 479.594 kb on + strand, within IAI47_02325 at 479.594 kb on + strand, within IAI47_02325 at 479.594 kb on + strand, within IAI47_02325 at 479.595 kb on - strand, within IAI47_02325 at 479.595 kb on - strand, within IAI47_02325 at 479.595 kb on - strand, within IAI47_02325 at 479.595 kb on - strand, within IAI47_02325 at 479.595 kb on - strand, within IAI47_02325 at 479.595 kb on - strand, within IAI47_02325 at 479.600 kb on + strand, within IAI47_02325 at 479.600 kb on + strand, within IAI47_02325 at 479.601 kb on - strand, within IAI47_02325 at 479.601 kb on - strand, within IAI47_02325 at 479.619 kb on + strand, within IAI47_02325 at 479.665 kb on + strand, within IAI47_02325 at 479.665 kb on + strand, within IAI47_02325 at 479.666 kb on - strand, within IAI47_02325 at 479.666 kb on - strand, within IAI47_02325 at 479.668 kb on + strand, within IAI47_02325 at 479.668 kb on + strand, within IAI47_02325 at 479.668 kb on + strand, within IAI47_02325 at 479.668 kb on + strand, within IAI47_02325 at 479.669 kb on - strand, within IAI47_02325 at 479.669 kb on - strand, within IAI47_02325 at 479.669 kb on - strand, within IAI47_02325 at 479.669 kb on - strand, within IAI47_02325 at 479.669 kb on - strand, within IAI47_02325 at 479.669 kb on - strand, within IAI47_02325 at 479.671 kb on + strand, within IAI47_02325 at 479.671 kb on + strand, within IAI47_02325 at 479.671 kb on + strand, within IAI47_02325 at 479.671 kb on + strand, within IAI47_02325 at 479.672 kb on - strand, within IAI47_02325 at 479.672 kb on - strand, within IAI47_02325 at 479.672 kb on - strand, within IAI47_02325 at 479.724 kb on + strand, within IAI47_02325 at 479.724 kb on + strand, within IAI47_02325 at 479.724 kb on + strand, within IAI47_02325 at 479.724 kb on + strand, within IAI47_02325 at 479.724 kb on + strand, within IAI47_02325 at 479.725 kb on - strand, within IAI47_02325 at 479.725 kb on - strand, within IAI47_02325 at 479.725 kb on - strand, within IAI47_02325 at 479.772 kb on + strand, within IAI47_02325 at 479.772 kb on + strand, within IAI47_02325 at 479.772 kb on + strand, within IAI47_02325 at 479.773 kb on - strand, within IAI47_02325 at 479.775 kb on + strand, within IAI47_02325 at 479.775 kb on + strand, within IAI47_02325 at 479.775 kb on + strand, within IAI47_02325 at 479.775 kb on + strand, within IAI47_02325 at 479.775 kb on + strand, within IAI47_02325 at 479.775 kb on + strand, within IAI47_02325 at 479.775 kb on + strand, within IAI47_02325 at 479.776 kb on - strand, within IAI47_02325 at 479.776 kb on - strand, within IAI47_02325 at 479.776 kb on - strand, within IAI47_02325 at 479.776 kb on - strand, within IAI47_02325 at 479.776 kb on - strand, within IAI47_02325 at 479.776 kb on - strand, within IAI47_02325 at 479.776 kb on - strand, within IAI47_02325 at 479.776 kb on - strand, within IAI47_02325 at 479.808 kb on + strand, within IAI47_02325 at 479.808 kb on + strand, within IAI47_02325 at 479.809 kb on - strand, within IAI47_02325 at 479.809 kb on - strand, within IAI47_02325 at 479.809 kb on - strand, within IAI47_02325 at 479.814 kb on + strand, within IAI47_02325 at 479.814 kb on + strand, within IAI47_02325 at 479.814 kb on + strand, within IAI47_02325 at 479.815 kb on - strand, within IAI47_02325 at 479.922 kb on + strand, within IAI47_02325 at 479.923 kb on - strand, within IAI47_02325 at 479.923 kb on - strand, within IAI47_02325 at 479.923 kb on - strand, within IAI47_02325 at 479.961 kb on + strand, within IAI47_02325 at 479.961 kb on + strand, within IAI47_02325 at 479.962 kb on - strand, within IAI47_02325 at 479.962 kb on - strand, within IAI47_02325 at 479.994 kb on + strand at 479.994 kb on + strand at 479.994 kb on + strand at 479.994 kb on + strand at 479.994 kb on + strand at 479.995 kb on - strand at 479.995 kb on - strand at 479.996 kb on + strand at 479.996 kb on + strand at 479.997 kb on - strand at 480.004 kb on + strand at 480.004 kb on + strand at 480.064 kb on - strand at 480.064 kb on - strand at 480.124 kb on + strand at 480.124 kb on + strand at 480.124 kb on + strand at 480.124 kb on + strand at 480.125 kb on - strand at 480.125 kb on - strand at 480.125 kb on - strand at 480.125 kb on - strand at 480.126 kb on + strand at 480.127 kb on - strand at 480.138 kb on + strand at 480.138 kb on + strand at 480.181 kb on - strand at 480.181 kb on - strand at 480.181 kb on - strand at 480.181 kb on - strand at 480.183 kb on - strand at 480.183 kb on - strand at 480.183 kb on - strand at 480.183 kb on - strand at 480.183 kb on - strand at 480.394 kb on + strand at 480.394 kb on + strand at 480.394 kb on + strand at 480.394 kb on + strand at 480.394 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 479,222 + +0.1 479,222 + -0.7 479,223 - -0.6 479,223 - +0.3 479,253 + -1.0 479,254 - -1.4 479,254 - +0.3 479,276 + IAI47_02320 0.14 -1.3 479,276 + IAI47_02320 0.14 +2.0 479,276 + IAI47_02320 0.14 -1.0 479,276 + IAI47_02320 0.14 +0.6 479,276 + IAI47_02320 0.14 -0.0 479,276 + IAI47_02320 0.14 +1.6 479,276 + IAI47_02320 0.14 +1.5 479,276 + IAI47_02320 0.14 +0.2 479,277 - IAI47_02320 0.14 -0.8 479,277 - IAI47_02320 0.14 -0.2 479,277 - IAI47_02320 0.14 +0.7 479,277 - IAI47_02320 0.14 +0.6 479,277 - IAI47_02320 0.14 +0.3 479,277 - IAI47_02320 0.14 +0.5 479,277 - IAI47_02320 0.14 +1.6 479,277 - IAI47_02320 0.14 +0.9 479,277 - IAI47_02320 0.14 -1.5 479,361 + IAI47_02320 0.47 -0.5 479,440 + IAI47_02320 0.78 -2.6 479,481 + +0.8 479,481 + -2.4 479,495 + -0.8 479,495 + -0.5 479,495 + -0.2 479,495 + -1.2 479,495 + -0.6 479,495 + +0.4 479,496 - +0.8 479,496 - -0.8 479,496 - +0.2 479,496 - +1.4 479,496 - -0.8 479,516 + +3.0 479,516 + -0.6 479,516 + +2.6 479,516 + -0.5 479,516 + -0.5 479,516 + -1.1 479,516 + -1.4 479,516 + +0.4 479,516 + +0.7 479,516 + +0.1 479,516 + +1.2 479,516 + -1.5 479,516 + -0.8 479,517 - -0.1 479,517 - -0.7 479,517 - +0.7 479,517 - +0.4 479,517 - -0.0 479,517 - +0.2 479,517 - +0.5 479,517 - -1.4 479,517 - -1.0 479,517 - -0.3 479,517 - -0.0 479,517 - +1.5 479,517 - -0.1 479,517 - +0.6 479,517 - +0.9 479,517 - -0.6 479,532 - IAI47_02325 0.12 +1.7 479,532 - IAI47_02325 0.12 +0.4 479,536 + IAI47_02325 0.13 +0.7 479,537 - IAI47_02325 0.13 +1.4 479,537 - IAI47_02325 0.13 +1.2 479,559 + IAI47_02325 0.17 -0.5 479,560 - IAI47_02325 0.17 +0.8 479,594 + IAI47_02325 0.23 +0.7 479,594 + IAI47_02325 0.23 -0.1 479,594 + IAI47_02325 0.23 +0.5 479,595 - IAI47_02325 0.24 -1.0 479,595 - IAI47_02325 0.24 +1.3 479,595 - IAI47_02325 0.24 +1.0 479,595 - IAI47_02325 0.24 -0.9 479,595 - IAI47_02325 0.24 -0.7 479,595 - IAI47_02325 0.24 +1.2 479,600 + IAI47_02325 0.25 +0.1 479,600 + IAI47_02325 0.25 -0.1 479,601 - IAI47_02325 0.25 +0.4 479,601 - IAI47_02325 0.25 +0.9 479,619 + IAI47_02325 0.28 -0.2 479,665 + IAI47_02325 0.36 +0.5 479,665 + IAI47_02325 0.36 -1.2 479,666 - IAI47_02325 0.36 +0.7 479,666 - IAI47_02325 0.36 -0.1 479,668 + IAI47_02325 0.37 +2.0 479,668 + IAI47_02325 0.37 -0.4 479,668 + IAI47_02325 0.37 +0.8 479,668 + IAI47_02325 0.37 -0.9 479,669 - IAI47_02325 0.37 -0.2 479,669 - IAI47_02325 0.37 +1.4 479,669 - IAI47_02325 0.37 +0.4 479,669 - IAI47_02325 0.37 -1.1 479,669 - IAI47_02325 0.37 +0.6 479,669 - IAI47_02325 0.37 +0.3 479,671 + IAI47_02325 0.37 +0.2 479,671 + IAI47_02325 0.37 -0.8 479,671 + IAI47_02325 0.37 +0.2 479,671 + IAI47_02325 0.37 +1.4 479,672 - IAI47_02325 0.37 +0.4 479,672 - IAI47_02325 0.37 -2.4 479,672 - IAI47_02325 0.37 -0.9 479,724 + IAI47_02325 0.47 -1.2 479,724 + IAI47_02325 0.47 +2.0 479,724 + IAI47_02325 0.47 +0.9 479,724 + IAI47_02325 0.47 -0.8 479,724 + IAI47_02325 0.47 -0.7 479,725 - IAI47_02325 0.47 +0.1 479,725 - IAI47_02325 0.47 +0.4 479,725 - IAI47_02325 0.47 -0.1 479,772 + IAI47_02325 0.55 +0.1 479,772 + IAI47_02325 0.55 -0.7 479,772 + IAI47_02325 0.55 +0.6 479,773 - IAI47_02325 0.56 -1.6 479,775 + IAI47_02325 0.56 +0.4 479,775 + IAI47_02325 0.56 -0.5 479,775 + IAI47_02325 0.56 +1.0 479,775 + IAI47_02325 0.56 +1.0 479,775 + IAI47_02325 0.56 +0.5 479,775 + IAI47_02325 0.56 +0.8 479,775 + IAI47_02325 0.56 -2.5 479,776 - IAI47_02325 0.56 -0.8 479,776 - IAI47_02325 0.56 -0.1 479,776 - IAI47_02325 0.56 +1.4 479,776 - IAI47_02325 0.56 +0.7 479,776 - IAI47_02325 0.56 +0.4 479,776 - IAI47_02325 0.56 -2.5 479,776 - IAI47_02325 0.56 +0.2 479,776 - IAI47_02325 0.56 -0.8 479,808 + IAI47_02325 0.62 +1.0 479,808 + IAI47_02325 0.62 +1.8 479,809 - IAI47_02325 0.62 +2.2 479,809 - IAI47_02325 0.62 +0.6 479,809 - IAI47_02325 0.62 -0.6 479,814 + IAI47_02325 0.63 -0.0 479,814 + IAI47_02325 0.63 +0.5 479,814 + IAI47_02325 0.63 -0.1 479,815 - IAI47_02325 0.63 +1.1 479,922 + IAI47_02325 0.83 -1.4 479,923 - IAI47_02325 0.83 -0.5 479,923 - IAI47_02325 0.83 +3.1 479,923 - IAI47_02325 0.83 +0.1 479,961 + IAI47_02325 0.90 +1.6 479,961 + IAI47_02325 0.90 -0.9 479,962 - IAI47_02325 0.90 -1.4 479,962 - IAI47_02325 0.90 +0.5 479,994 + +0.2 479,994 + -0.3 479,994 + +1.6 479,994 + -2.7 479,994 + -1.6 479,995 - -2.3 479,995 - +1.7 479,996 + +0.5 479,996 + +2.7 479,997 - -0.9 480,004 + -0.6 480,004 + -0.5 480,064 - +0.8 480,064 - +0.2 480,124 + +0.1 480,124 + -0.1 480,124 + +1.1 480,124 + -2.4 480,125 - +0.5 480,125 - +1.0 480,125 - -0.4 480,125 - +0.3 480,126 + -1.5 480,127 - -1.7 480,138 + +0.1 480,138 + +0.4 480,181 - +1.3 480,181 - +0.2 480,181 - +0.5 480,181 - -0.8 480,183 - -0.4 480,183 - -1.1 480,183 - +1.7 480,183 - +0.4 480,183 - -0.3 480,394 + -1.3 480,394 + +2.5 480,394 + -0.4 480,394 + -0.3 480,394 + +3.0
Or see this region's nucleotide sequence