Experiment: Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_00590 and IAI47_00595 are separated by 150 nucleotides IAI47_00595 and IAI47_00600 are separated by 49 nucleotides IAI47_00600 and IAI47_00605 are separated by 35 nucleotides IAI47_00605 and IAI47_00610 are separated by 119 nucleotides
IAI47_00590: IAI47_00590 - NupC/NupG family nucleoside CNT transporter, at 130,803 to 131,987
_00590
IAI47_00595: IAI47_00595 - ribonuclease PH, at 132,138 to 132,854
_00595
IAI47_00600: IAI47_00600 - orotate phosphoribosyltransferase, at 132,904 to 133,545
_00600
IAI47_00605: IAI47_00605 - nucleoid occlusion factor SlmA, at 133,581 to 134,177
_00605
IAI47_00610: IAI47_00610 - dUTP diphosphatase, at 134,297 to 134,755
_00610
Position (kb) 132
133
134 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 131.912 kb on + strand at 131.912 kb on + strand at 131.987 kb on + strand at 131.987 kb on + strand at 131.987 kb on + strand at 131.987 kb on + strand at 131.987 kb on + strand at 131.987 kb on + strand at 131.987 kb on + strand at 131.987 kb on + strand at 131.987 kb on + strand at 131.987 kb on + strand at 131.987 kb on + strand at 131.987 kb on + strand at 131.987 kb on + strand at 131.987 kb on + strand at 131.987 kb on + strand at 131.987 kb on + strand at 131.987 kb on + strand at 131.988 kb on - strand at 131.988 kb on - strand at 131.988 kb on - strand at 131.988 kb on - strand at 131.988 kb on - strand at 131.988 kb on - strand at 131.988 kb on - strand at 131.988 kb on - strand at 132.023 kb on + strand at 132.024 kb on - strand at 132.098 kb on + strand at 132.098 kb on + strand at 132.098 kb on + strand at 132.099 kb on - strand at 132.099 kb on - strand at 132.099 kb on - strand at 132.099 kb on - strand at 132.099 kb on - strand at 132.100 kb on + strand at 132.100 kb on + strand at 132.101 kb on - strand at 132.101 kb on - strand at 132.118 kb on + strand at 132.118 kb on + strand at 132.118 kb on + strand at 132.118 kb on + strand at 132.119 kb on - strand at 132.119 kb on - strand at 132.134 kb on - strand at 132.134 kb on - strand at 132.134 kb on - strand at 132.134 kb on - strand at 132.195 kb on + strand at 132.196 kb on - strand at 132.196 kb on - strand at 132.196 kb on - strand at 132.266 kb on - strand, within IAI47_00595 at 132.266 kb on - strand, within IAI47_00595 at 132.291 kb on - strand, within IAI47_00595 at 132.324 kb on + strand, within IAI47_00595 at 132.324 kb on + strand, within IAI47_00595 at 132.325 kb on - strand, within IAI47_00595 at 132.325 kb on - strand, within IAI47_00595 at 132.380 kb on + strand, within IAI47_00595 at 132.396 kb on - strand, within IAI47_00595 at 132.396 kb on - strand, within IAI47_00595 at 132.449 kb on + strand, within IAI47_00595 at 132.450 kb on - strand, within IAI47_00595 at 132.467 kb on + strand, within IAI47_00595 at 132.467 kb on + strand, within IAI47_00595 at 132.468 kb on - strand, within IAI47_00595 at 132.473 kb on + strand, within IAI47_00595 at 132.474 kb on - strand, within IAI47_00595 at 132.474 kb on - strand, within IAI47_00595 at 132.490 kb on + strand, within IAI47_00595 at 132.490 kb on + strand, within IAI47_00595 at 132.490 kb on + strand, within IAI47_00595 at 132.490 kb on + strand, within IAI47_00595 at 132.491 kb on - strand, within IAI47_00595 at 132.588 kb on - strand, within IAI47_00595 at 132.609 kb on - strand, within IAI47_00595 at 132.609 kb on - strand, within IAI47_00595 at 132.609 kb on - strand, within IAI47_00595 at 132.666 kb on + strand, within IAI47_00595 at 132.666 kb on + strand, within IAI47_00595 at 132.666 kb on + strand, within IAI47_00595 at 132.666 kb on + strand, within IAI47_00595 at 132.666 kb on + strand, within IAI47_00595 at 132.666 kb on + strand, within IAI47_00595 at 132.666 kb on + strand, within IAI47_00595 at 132.666 kb on + strand, within IAI47_00595 at 132.667 kb on - strand, within IAI47_00595 at 132.667 kb on - strand, within IAI47_00595 at 132.667 kb on - strand, within IAI47_00595 at 132.667 kb on - strand, within IAI47_00595 at 132.667 kb on - strand, within IAI47_00595 at 132.667 kb on - strand, within IAI47_00595 at 132.667 kb on - strand, within IAI47_00595 at 132.667 kb on - strand, within IAI47_00595 at 132.857 kb on + strand at 132.857 kb on + strand at 132.857 kb on + strand at 132.857 kb on + strand at 132.857 kb on + strand at 132.857 kb on + strand at 132.857 kb on + strand at 132.858 kb on - strand at 132.858 kb on - strand at 132.858 kb on - strand at 132.858 kb on - strand at 132.888 kb on - strand at 132.927 kb on + strand at 132.928 kb on - strand at 132.928 kb on - strand at 132.990 kb on + strand, within IAI47_00600 at 132.991 kb on - strand, within IAI47_00600 at 133.000 kb on + strand, within IAI47_00600 at 133.001 kb on - strand, within IAI47_00600 at 133.009 kb on - strand, within IAI47_00600 at 133.023 kb on + strand, within IAI47_00600 at 133.040 kb on + strand, within IAI47_00600 at 133.060 kb on + strand, within IAI47_00600 at 133.061 kb on - strand, within IAI47_00600 at 133.061 kb on - strand, within IAI47_00600 at 133.117 kb on + strand, within IAI47_00600 at 133.117 kb on + strand, within IAI47_00600 at 133.117 kb on + strand, within IAI47_00600 at 133.117 kb on + strand, within IAI47_00600 at 133.117 kb on + strand, within IAI47_00600 at 133.117 kb on + strand, within IAI47_00600 at 133.117 kb on + strand, within IAI47_00600 at 133.117 kb on + strand, within IAI47_00600 at 133.118 kb on - strand, within IAI47_00600 at 133.118 kb on - strand, within IAI47_00600 at 133.118 kb on - strand, within IAI47_00600 at 133.119 kb on + strand, within IAI47_00600 at 133.119 kb on + strand, within IAI47_00600 at 133.120 kb on - strand, within IAI47_00600 at 133.120 kb on - strand, within IAI47_00600 at 133.137 kb on + strand, within IAI47_00600 at 133.137 kb on + strand, within IAI47_00600 at 133.137 kb on + strand, within IAI47_00600 at 133.137 kb on + strand, within IAI47_00600 at 133.138 kb on - strand, within IAI47_00600 at 133.187 kb on - strand, within IAI47_00600 at 133.187 kb on - strand, within IAI47_00600 at 133.187 kb on - strand, within IAI47_00600 at 133.194 kb on + strand, within IAI47_00600 at 133.194 kb on + strand, within IAI47_00600 at 133.195 kb on - strand, within IAI47_00600 at 133.201 kb on - strand, within IAI47_00600 at 133.238 kb on + strand, within IAI47_00600 at 133.238 kb on + strand, within IAI47_00600 at 133.238 kb on + strand, within IAI47_00600 at 133.238 kb on + strand, within IAI47_00600 at 133.239 kb on - strand, within IAI47_00600 at 133.317 kb on + strand, within IAI47_00600 at 133.317 kb on + strand, within IAI47_00600 at 133.317 kb on + strand, within IAI47_00600 at 133.317 kb on + strand, within IAI47_00600 at 133.318 kb on - strand, within IAI47_00600 at 133.318 kb on - strand, within IAI47_00600 at 133.320 kb on + strand, within IAI47_00600 at 133.424 kb on - strand, within IAI47_00600 at 133.431 kb on + strand, within IAI47_00600 at 133.432 kb on - strand, within IAI47_00600 at 133.432 kb on - strand, within IAI47_00600 at 133.446 kb on + strand, within IAI47_00600 at 133.446 kb on + strand, within IAI47_00600 at 133.446 kb on + strand, within IAI47_00600 at 133.447 kb on - strand, within IAI47_00600 at 133.447 kb on - strand, within IAI47_00600 at 133.449 kb on + strand, within IAI47_00600 at 133.449 kb on + strand, within IAI47_00600 at 133.522 kb on + strand at 133.523 kb on - strand at 133.523 kb on - strand at 133.523 kb on - strand at 133.534 kb on + strand at 133.534 kb on + strand at 133.543 kb on + strand at 133.544 kb on - strand at 133.582 kb on + strand at 133.582 kb on + strand at 133.583 kb on - strand at 133.583 kb on - strand at 133.583 kb on - strand at 133.583 kb on - strand at 133.583 kb on - strand at 133.583 kb on - strand at 133.583 kb on - strand at 133.583 kb on - strand at 133.587 kb on + strand at 133.588 kb on - strand at 133.660 kb on + strand, within IAI47_00605 at 133.660 kb on + strand, within IAI47_00605 at 133.661 kb on - strand, within IAI47_00605 at 133.661 kb on - strand, within IAI47_00605 at 133.661 kb on - strand, within IAI47_00605 at 133.661 kb on - strand, within IAI47_00605 at 133.805 kb on + strand, within IAI47_00605 at 133.805 kb on + strand, within IAI47_00605 at 133.805 kb on + strand, within IAI47_00605 at 133.805 kb on + strand, within IAI47_00605 at 133.806 kb on - strand, within IAI47_00605 at 133.806 kb on - strand, within IAI47_00605 at 133.806 kb on - strand, within IAI47_00605 at 133.806 kb on - strand, within IAI47_00605 at 133.806 kb on - strand, within IAI47_00605 at 133.806 kb on - strand, within IAI47_00605 at 133.854 kb on + strand, within IAI47_00605 at 133.854 kb on + strand, within IAI47_00605 at 133.854 kb on + strand, within IAI47_00605 at 133.855 kb on - strand, within IAI47_00605 at 133.875 kb on - strand, within IAI47_00605 at 133.875 kb on - strand, within IAI47_00605 at 133.875 kb on - strand, within IAI47_00605 at 133.997 kb on + strand, within IAI47_00605 at 133.998 kb on - strand, within IAI47_00605 at 134.032 kb on + strand, within IAI47_00605 at 134.033 kb on - strand, within IAI47_00605 at 134.183 kb on + strand at 134.183 kb on + strand at 134.204 kb on + strand at 134.204 kb on + strand at 134.205 kb on - strand at 134.208 kb on + strand at 134.208 kb on + strand at 134.208 kb on + strand at 134.209 kb on - strand at 134.209 kb on - strand at 134.221 kb on + strand at 134.221 kb on + strand at 134.221 kb on + strand at 134.221 kb on + strand at 134.221 kb on + strand at 134.221 kb on + strand at 134.222 kb on - strand at 134.222 kb on - strand at 134.222 kb on - strand at 134.222 kb on - strand at 134.222 kb on - strand at 134.265 kb on + strand at 134.266 kb on - strand at 134.274 kb on + strand at 134.274 kb on + strand at 134.275 kb on - strand at 134.275 kb on - strand at 134.275 kb on - strand at 134.275 kb on - strand at 134.288 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 131,912 + -0.5 131,912 + -0.0 131,987 + -0.9 131,987 + -0.6 131,987 + -1.0 131,987 + +0.7 131,987 + +0.1 131,987 + +2.1 131,987 + +1.1 131,987 + -0.5 131,987 + +1.2 131,987 + +0.4 131,987 + +0.7 131,987 + +0.1 131,987 + -0.0 131,987 + -2.3 131,987 + +0.0 131,987 + +0.3 131,987 + -1.0 131,988 - -0.8 131,988 - -0.1 131,988 - +0.5 131,988 - -0.3 131,988 - -1.0 131,988 - -0.9 131,988 - +0.3 131,988 - +2.1 132,023 + +1.3 132,024 - -0.3 132,098 + +0.3 132,098 + -1.5 132,098 + -0.3 132,099 - -1.7 132,099 - -1.7 132,099 - +0.9 132,099 - -0.2 132,099 - -0.1 132,100 + +0.1 132,100 + +1.7 132,101 - -1.1 132,101 - -0.1 132,118 + -0.0 132,118 + -0.4 132,118 + -0.7 132,118 + +0.6 132,119 - -2.4 132,119 - -1.7 132,134 - -1.4 132,134 - +1.7 132,134 - -0.1 132,134 - +0.5 132,195 + -1.0 132,196 - +1.2 132,196 - +0.6 132,196 - -2.0 132,266 - IAI47_00595 0.18 +0.9 132,266 - IAI47_00595 0.18 +1.4 132,291 - IAI47_00595 0.21 -0.4 132,324 + IAI47_00595 0.26 -0.4 132,324 + IAI47_00595 0.26 +0.5 132,325 - IAI47_00595 0.26 -0.1 132,325 - IAI47_00595 0.26 -0.0 132,380 + IAI47_00595 0.34 +0.5 132,396 - IAI47_00595 0.36 -2.6 132,396 - IAI47_00595 0.36 +1.5 132,449 + IAI47_00595 0.43 +0.4 132,450 - IAI47_00595 0.44 +0.1 132,467 + IAI47_00595 0.46 +0.6 132,467 + IAI47_00595 0.46 -0.7 132,468 - IAI47_00595 0.46 -1.1 132,473 + IAI47_00595 0.47 -0.8 132,474 - IAI47_00595 0.47 +0.6 132,474 - IAI47_00595 0.47 +0.5 132,490 + IAI47_00595 0.49 -1.0 132,490 + IAI47_00595 0.49 +0.2 132,490 + IAI47_00595 0.49 +0.9 132,490 + IAI47_00595 0.49 -0.5 132,491 - IAI47_00595 0.49 -0.1 132,588 - IAI47_00595 0.63 -0.5 132,609 - IAI47_00595 0.66 -0.8 132,609 - IAI47_00595 0.66 +0.2 132,609 - IAI47_00595 0.66 +0.3 132,666 + IAI47_00595 0.74 -1.3 132,666 + IAI47_00595 0.74 -0.8 132,666 + IAI47_00595 0.74 -2.8 132,666 + IAI47_00595 0.74 +0.4 132,666 + IAI47_00595 0.74 -0.4 132,666 + IAI47_00595 0.74 -0.2 132,666 + IAI47_00595 0.74 -0.3 132,666 + IAI47_00595 0.74 -2.6 132,667 - IAI47_00595 0.74 +1.4 132,667 - IAI47_00595 0.74 +0.6 132,667 - IAI47_00595 0.74 +0.6 132,667 - IAI47_00595 0.74 -0.2 132,667 - IAI47_00595 0.74 +0.5 132,667 - IAI47_00595 0.74 -2.1 132,667 - IAI47_00595 0.74 -0.7 132,667 - IAI47_00595 0.74 -0.4 132,857 + -0.3 132,857 + -1.4 132,857 + +1.0 132,857 + +0.5 132,857 + +0.2 132,857 + +0.9 132,857 + -0.6 132,858 - -2.6 132,858 - -0.1 132,858 - -3.0 132,858 - +1.5 132,888 - +0.6 132,927 + +1.2 132,928 - +1.5 132,928 - +1.2 132,990 + IAI47_00600 0.13 -1.6 132,991 - IAI47_00600 0.14 +0.2 133,000 + IAI47_00600 0.15 -0.1 133,001 - IAI47_00600 0.15 -1.4 133,009 - IAI47_00600 0.16 -0.8 133,023 + IAI47_00600 0.19 -0.5 133,040 + IAI47_00600 0.21 +0.1 133,060 + IAI47_00600 0.24 -0.3 133,061 - IAI47_00600 0.24 -0.5 133,061 - IAI47_00600 0.24 -0.4 133,117 + IAI47_00600 0.33 +0.1 133,117 + IAI47_00600 0.33 +0.1 133,117 + IAI47_00600 0.33 -0.3 133,117 + IAI47_00600 0.33 +1.8 133,117 + IAI47_00600 0.33 +1.0 133,117 + IAI47_00600 0.33 -1.1 133,117 + IAI47_00600 0.33 +3.3 133,117 + IAI47_00600 0.33 -2.4 133,118 - IAI47_00600 0.33 -0.3 133,118 - IAI47_00600 0.33 -1.5 133,118 - IAI47_00600 0.33 +1.0 133,119 + IAI47_00600 0.33 +0.5 133,119 + IAI47_00600 0.33 +2.0 133,120 - IAI47_00600 0.34 +1.9 133,120 - IAI47_00600 0.34 +0.2 133,137 + IAI47_00600 0.36 -0.4 133,137 + IAI47_00600 0.36 +0.0 133,137 + IAI47_00600 0.36 +1.5 133,137 + IAI47_00600 0.36 -0.3 133,138 - IAI47_00600 0.36 -0.7 133,187 - IAI47_00600 0.44 -0.0 133,187 - IAI47_00600 0.44 +0.5 133,187 - IAI47_00600 0.44 +0.5 133,194 + IAI47_00600 0.45 -0.7 133,194 + IAI47_00600 0.45 -0.2 133,195 - IAI47_00600 0.45 +2.9 133,201 - IAI47_00600 0.46 +0.2 133,238 + IAI47_00600 0.52 +0.8 133,238 + IAI47_00600 0.52 +0.8 133,238 + IAI47_00600 0.52 -1.1 133,238 + IAI47_00600 0.52 +0.6 133,239 - IAI47_00600 0.52 +0.0 133,317 + IAI47_00600 0.64 +1.1 133,317 + IAI47_00600 0.64 +0.7 133,317 + IAI47_00600 0.64 +0.5 133,317 + IAI47_00600 0.64 -0.4 133,318 - IAI47_00600 0.64 -0.5 133,318 - IAI47_00600 0.64 +0.1 133,320 + IAI47_00600 0.65 -2.2 133,424 - IAI47_00600 0.81 -0.0 133,431 + IAI47_00600 0.82 +1.1 133,432 - IAI47_00600 0.82 +0.2 133,432 - IAI47_00600 0.82 -0.7 133,446 + IAI47_00600 0.84 -1.2 133,446 + IAI47_00600 0.84 -0.3 133,446 + IAI47_00600 0.84 +0.6 133,447 - IAI47_00600 0.85 +0.8 133,447 - IAI47_00600 0.85 +0.3 133,449 + IAI47_00600 0.85 +0.7 133,449 + IAI47_00600 0.85 -1.9 133,522 + +0.5 133,523 - -1.4 133,523 - +1.7 133,523 - -1.5 133,534 + +1.6 133,534 + +0.8 133,543 + +0.6 133,544 - +1.0 133,582 + +1.1 133,582 + +0.4 133,583 - -0.8 133,583 - -0.5 133,583 - -1.8 133,583 - +0.2 133,583 - +1.5 133,583 - -1.1 133,583 - -1.2 133,583 - -2.6 133,587 + +0.5 133,588 - -0.4 133,660 + IAI47_00605 0.13 -1.1 133,660 + IAI47_00605 0.13 -1.2 133,661 - IAI47_00605 0.13 +0.2 133,661 - IAI47_00605 0.13 -0.0 133,661 - IAI47_00605 0.13 -0.9 133,661 - IAI47_00605 0.13 -1.5 133,805 + IAI47_00605 0.38 -0.9 133,805 + IAI47_00605 0.38 +0.8 133,805 + IAI47_00605 0.38 -2.1 133,805 + IAI47_00605 0.38 -1.5 133,806 - IAI47_00605 0.38 -0.9 133,806 - IAI47_00605 0.38 -0.7 133,806 - IAI47_00605 0.38 -2.3 133,806 - IAI47_00605 0.38 +0.1 133,806 - IAI47_00605 0.38 +0.1 133,806 - IAI47_00605 0.38 +0.3 133,854 + IAI47_00605 0.46 -0.4 133,854 + IAI47_00605 0.46 +1.9 133,854 + IAI47_00605 0.46 +0.3 133,855 - IAI47_00605 0.46 +0.1 133,875 - IAI47_00605 0.49 -0.9 133,875 - IAI47_00605 0.49 -0.5 133,875 - IAI47_00605 0.49 +0.7 133,997 + IAI47_00605 0.70 +0.5 133,998 - IAI47_00605 0.70 +0.3 134,032 + IAI47_00605 0.76 +0.1 134,033 - IAI47_00605 0.76 -0.2 134,183 + +0.2 134,183 + +0.4 134,204 + -1.3 134,204 + -0.1 134,205 - -1.4 134,208 + +1.0 134,208 + +0.8 134,208 + +1.2 134,209 - -1.5 134,209 - +0.8 134,221 + -2.2 134,221 + -1.6 134,221 + +0.7 134,221 + +1.8 134,221 + -0.4 134,221 + +0.3 134,222 - +1.2 134,222 - +0.3 134,222 - +1.6 134,222 - -2.3 134,222 - +1.0 134,265 + -0.3 134,266 - -2.0 134,274 + -0.5 134,274 + -1.1 134,275 - +1.3 134,275 - -0.9 134,275 - -0.2 134,275 - -0.3 134,288 + -1.3
Or see this region's nucleotide sequence