Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20955 and IAI47_20960 are separated by 335 nucleotides IAI47_20960 and IAI47_20965 are separated by 123 nucleotides IAI47_20965 and IAI47_20970 are separated by 105 nucleotides
IAI47_20955: IAI47_20955 - M48 family metallopeptidase, at 438,353 to 440,212
_20955
IAI47_20960: IAI47_20960 - LysR family transcriptional regulator, at 440,548 to 441,432
_20960
IAI47_20965: IAI47_20965 - glutathione transferase GstA, at 441,556 to 442,161
_20965
IAI47_20970: IAI47_20970 - alpha/beta hydrolase, at 442,267 to 442,977
_20970
Position (kb) 440
441
442 Strain fitness (log2 ratio) -2
-1
0
1
2 at 439.555 kb on + strand, within IAI47_20955 at 439.555 kb on + strand, within IAI47_20955 at 439.555 kb on + strand, within IAI47_20955 at 439.556 kb on - strand, within IAI47_20955 at 439.556 kb on - strand, within IAI47_20955 at 439.556 kb on - strand, within IAI47_20955 at 439.556 kb on - strand, within IAI47_20955 at 439.562 kb on - strand, within IAI47_20955 at 439.603 kb on + strand, within IAI47_20955 at 439.604 kb on - strand, within IAI47_20955 at 439.604 kb on - strand, within IAI47_20955 at 439.685 kb on + strand, within IAI47_20955 at 439.706 kb on + strand, within IAI47_20955 at 439.706 kb on + strand, within IAI47_20955 at 439.707 kb on - strand, within IAI47_20955 at 439.707 kb on - strand, within IAI47_20955 at 439.796 kb on + strand, within IAI47_20955 at 439.796 kb on + strand, within IAI47_20955 at 439.797 kb on - strand, within IAI47_20955 at 439.904 kb on + strand, within IAI47_20955 at 439.913 kb on - strand, within IAI47_20955 at 439.992 kb on + strand, within IAI47_20955 at 439.993 kb on - strand, within IAI47_20955 at 440.015 kb on - strand, within IAI47_20955 at 440.036 kb on + strand at 440.036 kb on + strand at 440.036 kb on + strand at 440.037 kb on - strand at 440.037 kb on - strand at 440.037 kb on - strand at 440.040 kb on + strand at 440.040 kb on + strand at 440.040 kb on + strand at 440.040 kb on + strand at 440.041 kb on - strand at 440.041 kb on - strand at 440.068 kb on + strand at 440.122 kb on + strand at 440.122 kb on + strand at 440.128 kb on + strand at 440.172 kb on + strand at 440.173 kb on - strand at 440.210 kb on + strand at 440.267 kb on - strand at 440.295 kb on + strand at 440.295 kb on + strand at 440.295 kb on + strand at 440.295 kb on + strand at 440.296 kb on - strand at 440.296 kb on - strand at 440.296 kb on - strand at 440.296 kb on - strand at 440.296 kb on - strand at 440.296 kb on - strand at 440.296 kb on - strand at 440.317 kb on + strand at 440.387 kb on + strand at 440.387 kb on + strand at 440.436 kb on + strand at 440.436 kb on + strand at 440.437 kb on - strand at 440.468 kb on + strand at 440.470 kb on - strand at 440.470 kb on - strand at 440.470 kb on - strand at 440.470 kb on - strand at 440.596 kb on + strand at 440.597 kb on - strand at 440.597 kb on - strand at 440.657 kb on - strand, within IAI47_20960 at 440.956 kb on + strand, within IAI47_20960 at 440.956 kb on + strand, within IAI47_20960 at 440.957 kb on - strand, within IAI47_20960 at 440.959 kb on + strand, within IAI47_20960 at 440.959 kb on + strand, within IAI47_20960 at 440.959 kb on + strand, within IAI47_20960 at 440.959 kb on + strand, within IAI47_20960 at 440.959 kb on + strand, within IAI47_20960 at 441.090 kb on + strand, within IAI47_20960 at 441.120 kb on + strand, within IAI47_20960 at 441.153 kb on + strand, within IAI47_20960 at 441.153 kb on + strand, within IAI47_20960 at 441.167 kb on - strand, within IAI47_20960 at 441.198 kb on + strand, within IAI47_20960 at 441.199 kb on - strand, within IAI47_20960 at 441.199 kb on - strand, within IAI47_20960 at 441.262 kb on - strand, within IAI47_20960 at 441.262 kb on - strand, within IAI47_20960 at 441.313 kb on + strand, within IAI47_20960 at 441.313 kb on + strand, within IAI47_20960 at 441.313 kb on + strand, within IAI47_20960 at 441.314 kb on - strand, within IAI47_20960 at 441.314 kb on - strand, within IAI47_20960 at 441.381 kb on + strand at 441.382 kb on - strand at 441.382 kb on - strand at 441.382 kb on - strand at 441.382 kb on - strand at 441.432 kb on + strand at 441.432 kb on + strand at 441.463 kb on + strand at 441.466 kb on + strand at 441.507 kb on + strand at 441.507 kb on + strand at 441.507 kb on + strand at 441.508 kb on - strand at 441.508 kb on - strand at 441.508 kb on - strand at 441.508 kb on - strand at 441.537 kb on + strand at 441.537 kb on + strand at 441.538 kb on - strand at 441.538 kb on - strand at 441.565 kb on + strand at 441.565 kb on + strand at 441.623 kb on + strand, within IAI47_20965 at 441.624 kb on - strand, within IAI47_20965 at 441.639 kb on + strand, within IAI47_20965 at 441.706 kb on + strand, within IAI47_20965 at 441.706 kb on + strand, within IAI47_20965 at 441.706 kb on + strand, within IAI47_20965 at 441.706 kb on + strand, within IAI47_20965 at 441.707 kb on - strand, within IAI47_20965 at 441.707 kb on - strand, within IAI47_20965 at 441.707 kb on - strand, within IAI47_20965 at 441.707 kb on - strand, within IAI47_20965 at 441.740 kb on + strand, within IAI47_20965 at 441.741 kb on - strand, within IAI47_20965 at 441.741 kb on - strand, within IAI47_20965 at 441.741 kb on - strand, within IAI47_20965 at 441.769 kb on + strand, within IAI47_20965 at 441.769 kb on + strand, within IAI47_20965 at 441.770 kb on - strand, within IAI47_20965 at 441.770 kb on - strand, within IAI47_20965 at 441.849 kb on + strand, within IAI47_20965 at 441.849 kb on + strand, within IAI47_20965 at 441.850 kb on - strand, within IAI47_20965 at 441.850 kb on - strand, within IAI47_20965 at 441.850 kb on - strand, within IAI47_20965 at 441.855 kb on + strand, within IAI47_20965 at 441.855 kb on + strand, within IAI47_20965 at 441.856 kb on - strand, within IAI47_20965 at 441.859 kb on - strand, within IAI47_20965 at 441.894 kb on + strand, within IAI47_20965 at 441.894 kb on + strand, within IAI47_20965 at 441.895 kb on - strand, within IAI47_20965 at 441.959 kb on - strand, within IAI47_20965 at 441.959 kb on - strand, within IAI47_20965 at 441.959 kb on - strand, within IAI47_20965 at 441.982 kb on - strand, within IAI47_20965 at 442.024 kb on + strand, within IAI47_20965 at 442.025 kb on - strand, within IAI47_20965 at 442.025 kb on - strand, within IAI47_20965 at 442.025 kb on - strand, within IAI47_20965 at 442.025 kb on - strand, within IAI47_20965 at 442.025 kb on - strand, within IAI47_20965 at 442.030 kb on + strand, within IAI47_20965 at 442.030 kb on + strand, within IAI47_20965 at 442.030 kb on + strand, within IAI47_20965 at 442.030 kb on + strand, within IAI47_20965 at 442.030 kb on + strand, within IAI47_20965 at 442.031 kb on - strand, within IAI47_20965 at 442.031 kb on - strand, within IAI47_20965 at 442.031 kb on - strand, within IAI47_20965 at 442.139 kb on + strand at 442.140 kb on - strand at 442.159 kb on + strand at 442.227 kb on + strand at 442.227 kb on + strand at 442.227 kb on + strand at 442.227 kb on + strand at 442.228 kb on - strand at 442.228 kb on - strand at 442.228 kb on - strand at 442.267 kb on - strand at 442.334 kb on + strand at 442.334 kb on + strand at 442.334 kb on + strand at 442.335 kb on - strand at 442.335 kb on - strand at 442.337 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 439,555 + IAI47_20955 0.65 -0.9 439,555 + IAI47_20955 0.65 +0.6 439,555 + IAI47_20955 0.65 -1.2 439,556 - IAI47_20955 0.65 -0.2 439,556 - IAI47_20955 0.65 +0.1 439,556 - IAI47_20955 0.65 -1.6 439,556 - IAI47_20955 0.65 -1.8 439,562 - IAI47_20955 0.65 -0.0 439,603 + IAI47_20955 0.67 -1.2 439,604 - IAI47_20955 0.67 -0.3 439,604 - IAI47_20955 0.67 -0.1 439,685 + IAI47_20955 0.72 -0.7 439,706 + IAI47_20955 0.73 -1.4 439,706 + IAI47_20955 0.73 +0.9 439,707 - IAI47_20955 0.73 -1.1 439,707 - IAI47_20955 0.73 +0.4 439,796 + IAI47_20955 0.78 +0.5 439,796 + IAI47_20955 0.78 -0.6 439,797 - IAI47_20955 0.78 +1.1 439,904 + IAI47_20955 0.83 +1.3 439,913 - IAI47_20955 0.84 -0.2 439,992 + IAI47_20955 0.88 -0.5 439,993 - IAI47_20955 0.88 -0.1 440,015 - IAI47_20955 0.89 -1.4 440,036 + -1.1 440,036 + +1.1 440,036 + -0.4 440,037 - -0.5 440,037 - -0.5 440,037 - +1.0 440,040 + -0.1 440,040 + +0.1 440,040 + -1.7 440,040 + -1.1 440,041 - +1.4 440,041 - -0.2 440,068 + +0.3 440,122 + +0.8 440,122 + -1.4 440,128 + +1.4 440,172 + -0.5 440,173 - -0.5 440,210 + +1.3 440,267 - -1.7 440,295 + -0.3 440,295 + -0.4 440,295 + +0.0 440,295 + +2.2 440,296 - -1.0 440,296 - +1.4 440,296 - +0.7 440,296 - -0.1 440,296 - +1.8 440,296 - -1.5 440,296 - -0.1 440,317 + +0.1 440,387 + -1.1 440,387 + +1.5 440,436 + +0.2 440,436 + +2.1 440,437 - -0.0 440,468 + -1.5 440,470 - +0.4 440,470 - -0.0 440,470 - -0.3 440,470 - +0.8 440,596 + -1.0 440,597 - +0.0 440,597 - -1.0 440,657 - IAI47_20960 0.12 -0.5 440,956 + IAI47_20960 0.46 -0.8 440,956 + IAI47_20960 0.46 +0.3 440,957 - IAI47_20960 0.46 +0.9 440,959 + IAI47_20960 0.46 -0.5 440,959 + IAI47_20960 0.46 +0.9 440,959 + IAI47_20960 0.46 -0.1 440,959 + IAI47_20960 0.46 +0.3 440,959 + IAI47_20960 0.46 +0.5 441,090 + IAI47_20960 0.61 +0.9 441,120 + IAI47_20960 0.65 -0.1 441,153 + IAI47_20960 0.68 -0.4 441,153 + IAI47_20960 0.68 -2.1 441,167 - IAI47_20960 0.70 +0.1 441,198 + IAI47_20960 0.73 +1.1 441,199 - IAI47_20960 0.74 -0.2 441,199 - IAI47_20960 0.74 +0.2 441,262 - IAI47_20960 0.81 +1.3 441,262 - IAI47_20960 0.81 -1.9 441,313 + IAI47_20960 0.86 +0.9 441,313 + IAI47_20960 0.86 -1.5 441,313 + IAI47_20960 0.86 -0.4 441,314 - IAI47_20960 0.87 +0.3 441,314 - IAI47_20960 0.87 -1.3 441,381 + -1.0 441,382 - -0.1 441,382 - -1.4 441,382 - -1.5 441,382 - +0.5 441,432 + -0.4 441,432 + +0.0 441,463 + -0.4 441,466 + +0.0 441,507 + -2.6 441,507 + -1.4 441,507 + +0.5 441,508 - -2.6 441,508 - +0.2 441,508 - +0.5 441,508 - -0.6 441,537 + +2.0 441,537 + -0.2 441,538 - +0.2 441,538 - +1.3 441,565 + +1.3 441,565 + +2.0 441,623 + IAI47_20965 0.11 +0.4 441,624 - IAI47_20965 0.11 +0.2 441,639 + IAI47_20965 0.14 -0.0 441,706 + IAI47_20965 0.25 +0.1 441,706 + IAI47_20965 0.25 +0.3 441,706 + IAI47_20965 0.25 -1.4 441,706 + IAI47_20965 0.25 +0.8 441,707 - IAI47_20965 0.25 -2.4 441,707 - IAI47_20965 0.25 -0.1 441,707 - IAI47_20965 0.25 +0.2 441,707 - IAI47_20965 0.25 -1.3 441,740 + IAI47_20965 0.30 +0.3 441,741 - IAI47_20965 0.31 +0.6 441,741 - IAI47_20965 0.31 -1.1 441,741 - IAI47_20965 0.31 -0.1 441,769 + IAI47_20965 0.35 +0.5 441,769 + IAI47_20965 0.35 -1.4 441,770 - IAI47_20965 0.35 -1.7 441,770 - IAI47_20965 0.35 -0.6 441,849 + IAI47_20965 0.48 -2.2 441,849 + IAI47_20965 0.48 +0.0 441,850 - IAI47_20965 0.49 -1.4 441,850 - IAI47_20965 0.49 -1.7 441,850 - IAI47_20965 0.49 -0.8 441,855 + IAI47_20965 0.49 -0.7 441,855 + IAI47_20965 0.49 +0.2 441,856 - IAI47_20965 0.50 -0.2 441,859 - IAI47_20965 0.50 +0.0 441,894 + IAI47_20965 0.56 +0.1 441,894 + IAI47_20965 0.56 +0.2 441,895 - IAI47_20965 0.56 +0.7 441,959 - IAI47_20965 0.67 +0.1 441,959 - IAI47_20965 0.67 -1.1 441,959 - IAI47_20965 0.67 -1.3 441,982 - IAI47_20965 0.70 +0.3 442,024 + IAI47_20965 0.77 +1.6 442,025 - IAI47_20965 0.77 -0.8 442,025 - IAI47_20965 0.77 +0.3 442,025 - IAI47_20965 0.77 -2.3 442,025 - IAI47_20965 0.77 +0.8 442,025 - IAI47_20965 0.77 +0.8 442,030 + IAI47_20965 0.78 +1.5 442,030 + IAI47_20965 0.78 -0.7 442,030 + IAI47_20965 0.78 +2.1 442,030 + IAI47_20965 0.78 +0.9 442,030 + IAI47_20965 0.78 -0.7 442,031 - IAI47_20965 0.78 -1.5 442,031 - IAI47_20965 0.78 +0.0 442,031 - IAI47_20965 0.78 -0.3 442,139 + -1.2 442,140 - -2.1 442,159 + -1.8 442,227 + -0.1 442,227 + +0.6 442,227 + -2.1 442,227 + +0.9 442,228 - +0.6 442,228 - -0.6 442,228 - +0.7 442,267 - -0.2 442,334 + +0.3 442,334 + -0.5 442,334 + -2.1 442,335 - +0.3 442,335 - -0.4 442,337 + +0.1
Or see this region's nucleotide sequence