Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20655 and IAI47_20660 overlap by 14 nucleotides IAI47_20660 and IAI47_20665 overlap by 4 nucleotides
IAI47_20655: IAI47_20655 - glycosyltransferase family 1 protein, at 380,651 to 381,946
_20655
IAI47_20660: IAI47_20660 - lycopene beta-cyclase CrtY, at 381,933 to 383,093
_20660
IAI47_20665: IAI47_20665 - phytoene desaturase, at 383,090 to 384,568
_20665
Position (kb) 381
382
383
384 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 381.009 kb on + strand, within IAI47_20655 at 381.010 kb on - strand, within IAI47_20655 at 381.010 kb on - strand, within IAI47_20655 at 381.107 kb on + strand, within IAI47_20655 at 381.108 kb on - strand, within IAI47_20655 at 381.108 kb on - strand, within IAI47_20655 at 381.220 kb on - strand, within IAI47_20655 at 381.278 kb on - strand, within IAI47_20655 at 381.380 kb on + strand, within IAI47_20655 at 381.381 kb on - strand, within IAI47_20655 at 381.381 kb on - strand, within IAI47_20655 at 381.381 kb on - strand, within IAI47_20655 at 381.454 kb on + strand, within IAI47_20655 at 381.496 kb on - strand, within IAI47_20655 at 381.655 kb on + strand, within IAI47_20655 at 381.758 kb on - strand, within IAI47_20655 at 381.758 kb on - strand, within IAI47_20655 at 381.758 kb on - strand, within IAI47_20655 at 381.812 kb on + strand, within IAI47_20655 at 381.812 kb on + strand, within IAI47_20655 at 381.813 kb on - strand, within IAI47_20655 at 381.813 kb on - strand, within IAI47_20655 at 381.813 kb on - strand, within IAI47_20655 at 381.813 kb on - strand, within IAI47_20655 at 381.813 kb on - strand, within IAI47_20655 at 381.813 kb on - strand, within IAI47_20655 at 381.813 kb on - strand, within IAI47_20655 at 381.919 kb on + strand at 381.919 kb on + strand at 381.919 kb on + strand at 381.919 kb on + strand at 381.919 kb on + strand at 381.920 kb on - strand at 381.920 kb on - strand at 381.920 kb on - strand at 381.932 kb on + strand at 381.932 kb on + strand at 381.932 kb on + strand at 381.933 kb on - strand at 381.933 kb on - strand at 381.933 kb on - strand at 381.933 kb on - strand at 381.933 kb on - strand at 381.933 kb on - strand at 381.950 kb on + strand at 381.951 kb on - strand at 381.951 kb on - strand at 381.951 kb on - strand at 381.951 kb on - strand at 382.030 kb on + strand at 382.030 kb on + strand at 382.031 kb on - strand at 382.316 kb on - strand, within IAI47_20660 at 382.350 kb on + strand, within IAI47_20660 at 382.350 kb on + strand, within IAI47_20660 at 382.374 kb on - strand, within IAI47_20660 at 382.489 kb on - strand, within IAI47_20660 at 382.489 kb on - strand, within IAI47_20660 at 382.512 kb on - strand, within IAI47_20660 at 382.529 kb on + strand, within IAI47_20660 at 382.530 kb on - strand, within IAI47_20660 at 382.530 kb on - strand, within IAI47_20660 at 382.536 kb on + strand, within IAI47_20660 at 382.536 kb on + strand, within IAI47_20660 at 382.536 kb on + strand, within IAI47_20660 at 382.536 kb on + strand, within IAI47_20660 at 382.536 kb on + strand, within IAI47_20660 at 382.536 kb on + strand, within IAI47_20660 at 382.536 kb on + strand, within IAI47_20660 at 382.536 kb on + strand, within IAI47_20660 at 382.536 kb on + strand, within IAI47_20660 at 382.537 kb on - strand, within IAI47_20660 at 382.537 kb on - strand, within IAI47_20660 at 382.537 kb on - strand, within IAI47_20660 at 382.537 kb on - strand, within IAI47_20660 at 382.537 kb on - strand, within IAI47_20660 at 382.537 kb on - strand, within IAI47_20660 at 382.538 kb on + strand, within IAI47_20660 at 382.538 kb on + strand, within IAI47_20660 at 382.538 kb on + strand, within IAI47_20660 at 382.539 kb on - strand, within IAI47_20660 at 382.539 kb on - strand, within IAI47_20660 at 382.581 kb on - strand, within IAI47_20660 at 382.590 kb on + strand, within IAI47_20660 at 382.590 kb on + strand, within IAI47_20660 at 382.591 kb on - strand, within IAI47_20660 at 382.625 kb on - strand, within IAI47_20660 at 382.625 kb on - strand, within IAI47_20660 at 382.625 kb on - strand, within IAI47_20660 at 382.656 kb on - strand, within IAI47_20660 at 382.656 kb on - strand, within IAI47_20660 at 382.700 kb on - strand, within IAI47_20660 at 382.736 kb on + strand, within IAI47_20660 at 382.736 kb on + strand, within IAI47_20660 at 382.737 kb on - strand, within IAI47_20660 at 382.746 kb on + strand, within IAI47_20660 at 382.747 kb on - strand, within IAI47_20660 at 382.803 kb on - strand, within IAI47_20660 at 382.880 kb on - strand, within IAI47_20660 at 382.928 kb on + strand, within IAI47_20660 at 382.941 kb on + strand, within IAI47_20660 at 383.010 kb on - strand at 383.014 kb on + strand at 383.015 kb on - strand at 383.015 kb on - strand at 383.050 kb on + strand at 383.051 kb on - strand at 383.051 kb on - strand at 383.051 kb on - strand at 383.057 kb on + strand at 383.057 kb on + strand at 383.057 kb on + strand at 383.057 kb on + strand at 383.058 kb on - strand at 383.058 kb on - strand at 383.058 kb on - strand at 383.060 kb on + strand at 383.060 kb on + strand at 383.060 kb on + strand at 383.060 kb on + strand at 383.060 kb on + strand at 383.060 kb on + strand at 383.060 kb on + strand at 383.060 kb on + strand at 383.060 kb on + strand at 383.060 kb on + strand at 383.061 kb on - strand at 383.061 kb on - strand at 383.061 kb on - strand at 383.061 kb on - strand at 383.061 kb on - strand at 383.062 kb on - strand at 383.095 kb on + strand at 383.095 kb on + strand at 383.095 kb on + strand at 383.096 kb on - strand at 383.096 kb on - strand at 383.096 kb on - strand at 383.106 kb on + strand at 383.106 kb on + strand at 383.106 kb on + strand at 383.106 kb on + strand at 383.107 kb on - strand at 383.210 kb on + strand at 383.210 kb on + strand at 383.210 kb on + strand at 383.210 kb on + strand at 383.211 kb on - strand at 383.211 kb on - strand at 383.211 kb on - strand at 383.216 kb on + strand at 383.216 kb on + strand at 383.216 kb on + strand at 383.216 kb on + strand at 383.217 kb on - strand at 383.217 kb on - strand at 383.217 kb on - strand at 383.217 kb on - strand at 383.217 kb on - strand at 383.217 kb on - strand at 383.217 kb on - strand at 383.232 kb on - strand at 383.257 kb on - strand, within IAI47_20665 at 383.348 kb on + strand, within IAI47_20665 at 383.349 kb on - strand, within IAI47_20665 at 383.376 kb on + strand, within IAI47_20665 at 383.376 kb on + strand, within IAI47_20665 at 383.377 kb on - strand, within IAI47_20665 at 383.385 kb on - strand, within IAI47_20665 at 383.428 kb on + strand, within IAI47_20665 at 383.463 kb on + strand, within IAI47_20665 at 383.463 kb on + strand, within IAI47_20665 at 383.463 kb on + strand, within IAI47_20665 at 383.464 kb on - strand, within IAI47_20665 at 383.468 kb on + strand, within IAI47_20665 at 383.468 kb on + strand, within IAI47_20665 at 383.468 kb on + strand, within IAI47_20665 at 383.469 kb on - strand, within IAI47_20665 at 383.496 kb on - strand, within IAI47_20665 at 383.496 kb on - strand, within IAI47_20665 at 383.569 kb on - strand, within IAI47_20665 at 383.588 kb on + strand, within IAI47_20665 at 383.588 kb on + strand, within IAI47_20665 at 383.606 kb on + strand, within IAI47_20665 at 383.606 kb on + strand, within IAI47_20665 at 383.606 kb on + strand, within IAI47_20665 at 383.607 kb on - strand, within IAI47_20665 at 383.607 kb on - strand, within IAI47_20665 at 383.607 kb on - strand, within IAI47_20665 at 383.607 kb on - strand, within IAI47_20665 at 383.607 kb on - strand, within IAI47_20665 at 383.607 kb on - strand, within IAI47_20665 at 383.607 kb on - strand, within IAI47_20665 at 383.607 kb on - strand, within IAI47_20665 at 383.691 kb on - strand, within IAI47_20665 at 383.691 kb on - strand, within IAI47_20665 at 383.802 kb on + strand, within IAI47_20665 at 383.802 kb on + strand, within IAI47_20665 at 383.803 kb on - strand, within IAI47_20665 at 383.803 kb on - strand, within IAI47_20665 at 383.803 kb on - strand, within IAI47_20665 at 383.845 kb on + strand, within IAI47_20665 at 383.927 kb on + strand, within IAI47_20665 at 383.927 kb on + strand, within IAI47_20665 at 383.927 kb on + strand, within IAI47_20665 at 383.928 kb on - strand, within IAI47_20665 at 383.928 kb on - strand, within IAI47_20665 at 383.928 kb on - strand, within IAI47_20665 at 383.930 kb on - strand, within IAI47_20665 at 383.937 kb on + strand, within IAI47_20665 at 383.986 kb on + strand, within IAI47_20665 at 383.987 kb on - strand, within IAI47_20665 at 383.998 kb on + strand, within IAI47_20665 at 383.999 kb on - strand, within IAI47_20665 at 384.017 kb on + strand, within IAI47_20665 at 384.018 kb on - strand, within IAI47_20665 at 384.018 kb on - strand, within IAI47_20665 at 384.058 kb on + strand, within IAI47_20665 at 384.058 kb on + strand, within IAI47_20665 at 384.090 kb on + strand, within IAI47_20665 at 384.090 kb on + strand, within IAI47_20665 at 384.091 kb on - strand, within IAI47_20665
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 381,009 + IAI47_20655 0.28 -2.2 381,010 - IAI47_20655 0.28 +1.4 381,010 - IAI47_20655 0.28 -0.3 381,107 + IAI47_20655 0.35 +1.1 381,108 - IAI47_20655 0.35 -0.6 381,108 - IAI47_20655 0.35 +0.3 381,220 - IAI47_20655 0.44 +0.8 381,278 - IAI47_20655 0.48 -1.0 381,380 + IAI47_20655 0.56 +1.5 381,381 - IAI47_20655 0.56 +0.5 381,381 - IAI47_20655 0.56 +0.8 381,381 - IAI47_20655 0.56 +0.7 381,454 + IAI47_20655 0.62 -1.2 381,496 - IAI47_20655 0.65 +0.5 381,655 + IAI47_20655 0.77 -0.5 381,758 - IAI47_20655 0.85 +2.6 381,758 - IAI47_20655 0.85 -0.3 381,758 - IAI47_20655 0.85 +0.1 381,812 + IAI47_20655 0.90 -0.1 381,812 + IAI47_20655 0.90 +1.3 381,813 - IAI47_20655 0.90 +0.6 381,813 - IAI47_20655 0.90 +0.0 381,813 - IAI47_20655 0.90 +0.4 381,813 - IAI47_20655 0.90 -2.1 381,813 - IAI47_20655 0.90 -1.4 381,813 - IAI47_20655 0.90 +1.3 381,813 - IAI47_20655 0.90 +0.9 381,919 + -0.1 381,919 + +0.0 381,919 + -0.1 381,919 + +0.5 381,919 + +1.0 381,920 - -1.0 381,920 - -0.7 381,920 - -0.8 381,932 + -2.2 381,932 + +0.5 381,932 + +0.4 381,933 - +0.1 381,933 - +0.1 381,933 - -1.4 381,933 - -0.6 381,933 - -0.5 381,933 - -0.7 381,950 + -0.3 381,951 - +0.7 381,951 - +0.5 381,951 - +0.2 381,951 - -0.5 382,030 + -2.7 382,030 + -1.0 382,031 - +0.6 382,316 - IAI47_20660 0.33 -1.1 382,350 + IAI47_20660 0.36 +2.1 382,350 + IAI47_20660 0.36 +0.5 382,374 - IAI47_20660 0.38 -0.2 382,489 - IAI47_20660 0.48 +0.8 382,489 - IAI47_20660 0.48 +2.1 382,512 - IAI47_20660 0.50 -0.4 382,529 + IAI47_20660 0.51 +1.6 382,530 - IAI47_20660 0.51 +0.9 382,530 - IAI47_20660 0.51 -0.5 382,536 + IAI47_20660 0.52 +3.9 382,536 + IAI47_20660 0.52 -0.7 382,536 + IAI47_20660 0.52 -1.2 382,536 + IAI47_20660 0.52 +1.2 382,536 + IAI47_20660 0.52 -0.3 382,536 + IAI47_20660 0.52 -1.0 382,536 + IAI47_20660 0.52 +0.8 382,536 + IAI47_20660 0.52 +0.8 382,536 + IAI47_20660 0.52 +0.1 382,537 - IAI47_20660 0.52 +1.1 382,537 - IAI47_20660 0.52 -0.3 382,537 - IAI47_20660 0.52 +1.3 382,537 - IAI47_20660 0.52 +0.8 382,537 - IAI47_20660 0.52 +1.2 382,537 - IAI47_20660 0.52 -1.4 382,538 + IAI47_20660 0.52 -2.1 382,538 + IAI47_20660 0.52 +1.9 382,538 + IAI47_20660 0.52 +0.4 382,539 - IAI47_20660 0.52 -1.0 382,539 - IAI47_20660 0.52 +0.6 382,581 - IAI47_20660 0.56 +0.4 382,590 + IAI47_20660 0.57 -0.1 382,590 + IAI47_20660 0.57 +1.1 382,591 - IAI47_20660 0.57 +0.2 382,625 - IAI47_20660 0.60 +2.3 382,625 - IAI47_20660 0.60 -1.0 382,625 - IAI47_20660 0.60 +1.0 382,656 - IAI47_20660 0.62 +0.6 382,656 - IAI47_20660 0.62 -0.2 382,700 - IAI47_20660 0.66 +0.4 382,736 + IAI47_20660 0.69 -0.6 382,736 + IAI47_20660 0.69 -0.6 382,737 - IAI47_20660 0.69 +1.0 382,746 + IAI47_20660 0.70 -1.5 382,747 - IAI47_20660 0.70 +1.7 382,803 - IAI47_20660 0.75 +0.9 382,880 - IAI47_20660 0.82 +0.8 382,928 + IAI47_20660 0.86 -0.1 382,941 + IAI47_20660 0.87 +0.6 383,010 - +0.0 383,014 + +0.8 383,015 - +0.8 383,015 - -1.2 383,050 + -0.1 383,051 - +0.9 383,051 - +0.4 383,051 - -1.7 383,057 + -0.4 383,057 + +0.3 383,057 + -1.0 383,057 + +0.5 383,058 - +0.7 383,058 - +1.5 383,058 - -2.3 383,060 + -0.6 383,060 + -0.1 383,060 + -1.2 383,060 + -0.4 383,060 + +0.5 383,060 + +0.2 383,060 + +1.0 383,060 + +0.9 383,060 + -1.0 383,060 + +1.7 383,061 - -0.3 383,061 - -0.8 383,061 - +0.3 383,061 - +0.2 383,061 - -1.6 383,062 - -2.2 383,095 + -0.4 383,095 + +0.1 383,095 + -0.4 383,096 - +0.5 383,096 - +0.5 383,096 - +0.9 383,106 + -0.9 383,106 + -0.5 383,106 + -0.2 383,106 + +0.4 383,107 - +0.2 383,210 + +0.8 383,210 + +1.1 383,210 + -0.3 383,210 + +1.5 383,211 - +1.0 383,211 - +0.1 383,211 - +1.1 383,216 + -0.2 383,216 + +0.0 383,216 + -1.1 383,216 + -0.5 383,217 - +0.3 383,217 - -1.3 383,217 - -0.5 383,217 - +0.6 383,217 - +0.5 383,217 - -0.3 383,217 - +0.8 383,232 - -0.5 383,257 - IAI47_20665 0.11 -0.5 383,348 + IAI47_20665 0.17 -0.8 383,349 - IAI47_20665 0.18 +0.1 383,376 + IAI47_20665 0.19 -1.0 383,376 + IAI47_20665 0.19 -0.1 383,377 - IAI47_20665 0.19 -0.2 383,385 - IAI47_20665 0.20 +0.3 383,428 + IAI47_20665 0.23 +0.3 383,463 + IAI47_20665 0.25 -0.4 383,463 + IAI47_20665 0.25 -0.0 383,463 + IAI47_20665 0.25 +0.1 383,464 - IAI47_20665 0.25 +0.5 383,468 + IAI47_20665 0.26 +0.6 383,468 + IAI47_20665 0.26 -0.5 383,468 + IAI47_20665 0.26 +1.6 383,469 - IAI47_20665 0.26 -0.7 383,496 - IAI47_20665 0.27 +0.5 383,496 - IAI47_20665 0.27 -0.2 383,569 - IAI47_20665 0.32 +3.1 383,588 + IAI47_20665 0.34 +1.4 383,588 + IAI47_20665 0.34 +0.2 383,606 + IAI47_20665 0.35 +0.6 383,606 + IAI47_20665 0.35 +0.4 383,606 + IAI47_20665 0.35 -0.6 383,607 - IAI47_20665 0.35 -1.5 383,607 - IAI47_20665 0.35 -0.6 383,607 - IAI47_20665 0.35 +0.6 383,607 - IAI47_20665 0.35 -1.4 383,607 - IAI47_20665 0.35 +2.3 383,607 - IAI47_20665 0.35 +1.0 383,607 - IAI47_20665 0.35 +0.9 383,607 - IAI47_20665 0.35 -0.9 383,691 - IAI47_20665 0.41 -1.2 383,691 - IAI47_20665 0.41 +3.1 383,802 + IAI47_20665 0.48 -1.7 383,802 + IAI47_20665 0.48 -0.1 383,803 - IAI47_20665 0.48 +0.7 383,803 - IAI47_20665 0.48 +1.3 383,803 - IAI47_20665 0.48 -0.3 383,845 + IAI47_20665 0.51 -0.7 383,927 + IAI47_20665 0.57 +1.9 383,927 + IAI47_20665 0.57 +2.1 383,927 + IAI47_20665 0.57 +1.2 383,928 - IAI47_20665 0.57 -0.1 383,928 - IAI47_20665 0.57 +0.3 383,928 - IAI47_20665 0.57 -0.8 383,930 - IAI47_20665 0.57 -1.0 383,937 + IAI47_20665 0.57 +1.1 383,986 + IAI47_20665 0.61 +0.4 383,987 - IAI47_20665 0.61 +0.7 383,998 + IAI47_20665 0.61 +2.3 383,999 - IAI47_20665 0.61 +1.3 384,017 + IAI47_20665 0.63 -0.3 384,018 - IAI47_20665 0.63 -1.4 384,018 - IAI47_20665 0.63 +0.6 384,058 + IAI47_20665 0.65 -0.5 384,058 + IAI47_20665 0.65 +1.8 384,090 + IAI47_20665 0.68 +0.6 384,090 + IAI47_20665 0.68 -0.9 384,091 - IAI47_20665 0.68 -0.4
Or see this region's nucleotide sequence