Experiment: Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_11520 and IAI47_11525 are separated by 394 nucleotides IAI47_11525 and IAI47_11530 overlap by 4 nucleotides
IAI47_11520: IAI47_11520 - type VI secretion system tip protein VgrG, at 2,436,413 to 2,437,974
_11520
IAI47_11525: IAI47_11525 - ADP-ribosylglycohydrolase family protein, at 2,438,369 to 2,439,460
_11525
IAI47_11530: IAI47_11530 - PAAR domain-containing protein, at 2,439,457 to 2,440,794
_11530
Position (kb)
2438
2439
2440 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2437.593 kb on - strand, within IAI47_11520 at 2437.593 kb on - strand, within IAI47_11520 at 2437.759 kb on - strand, within IAI47_11520 at 2438.043 kb on + strand at 2438.069 kb on + strand at 2438.279 kb on - strand at 2438.299 kb on - strand at 2438.329 kb on + strand at 2438.354 kb on + strand at 2439.471 kb on + strand at 2439.472 kb on - strand at 2439.473 kb on + strand at 2439.520 kb on - strand at 2439.540 kb on - strand at 2439.540 kb on - strand at 2439.541 kb on + strand at 2439.541 kb on + strand at 2439.542 kb on - strand at 2439.572 kb on - strand at 2439.572 kb on - strand at 2439.616 kb on + strand, within IAI47_11530 at 2439.617 kb on - strand, within IAI47_11530 at 2439.621 kb on + strand, within IAI47_11530 at 2439.621 kb on + strand, within IAI47_11530 at 2439.648 kb on + strand, within IAI47_11530 at 2439.648 kb on + strand, within IAI47_11530 at 2439.648 kb on + strand, within IAI47_11530 at 2439.649 kb on - strand, within IAI47_11530 at 2439.649 kb on - strand, within IAI47_11530 at 2439.649 kb on - strand, within IAI47_11530 at 2439.655 kb on - strand, within IAI47_11530 at 2439.679 kb on - strand, within IAI47_11530 at 2439.679 kb on - strand, within IAI47_11530 at 2439.730 kb on - strand, within IAI47_11530 at 2439.731 kb on + strand, within IAI47_11530 at 2439.745 kb on - strand, within IAI47_11530 at 2439.745 kb on - strand, within IAI47_11530 at 2439.745 kb on - strand, within IAI47_11530 at 2439.745 kb on - strand, within IAI47_11530 at 2439.747 kb on + strand, within IAI47_11530 at 2439.747 kb on + strand, within IAI47_11530 at 2439.774 kb on + strand, within IAI47_11530 at 2439.775 kb on - strand, within IAI47_11530 at 2439.775 kb on - strand, within IAI47_11530 at 2439.775 kb on - strand, within IAI47_11530 at 2439.775 kb on - strand, within IAI47_11530 at 2439.775 kb on - strand, within IAI47_11530 at 2439.775 kb on - strand, within IAI47_11530 at 2439.796 kb on + strand, within IAI47_11530 at 2439.796 kb on + strand, within IAI47_11530 at 2439.797 kb on - strand, within IAI47_11530 at 2439.797 kb on - strand, within IAI47_11530 at 2439.808 kb on + strand, within IAI47_11530 at 2439.826 kb on + strand, within IAI47_11530 at 2439.826 kb on + strand, within IAI47_11530 at 2439.826 kb on + strand, within IAI47_11530 at 2439.826 kb on + strand, within IAI47_11530 at 2439.826 kb on + strand, within IAI47_11530 at 2439.826 kb on + strand, within IAI47_11530 at 2439.827 kb on - strand, within IAI47_11530 at 2439.827 kb on - strand, within IAI47_11530 at 2439.827 kb on - strand, within IAI47_11530 at 2439.827 kb on - strand, within IAI47_11530 at 2439.827 kb on - strand, within IAI47_11530 at 2439.827 kb on - strand, within IAI47_11530 at 2439.827 kb on - strand, within IAI47_11530 at 2439.838 kb on + strand, within IAI47_11530 at 2439.839 kb on - strand, within IAI47_11530 at 2439.839 kb on - strand, within IAI47_11530 at 2439.839 kb on - strand, within IAI47_11530 at 2439.839 kb on - strand, within IAI47_11530 at 2439.839 kb on - strand, within IAI47_11530 at 2439.839 kb on - strand, within IAI47_11530 at 2439.849 kb on - strand, within IAI47_11530 at 2439.864 kb on + strand, within IAI47_11530 at 2439.866 kb on + strand, within IAI47_11530 at 2439.866 kb on + strand, within IAI47_11530 at 2439.866 kb on + strand, within IAI47_11530 at 2439.866 kb on + strand, within IAI47_11530 at 2439.867 kb on - strand, within IAI47_11530 at 2439.872 kb on + strand, within IAI47_11530 at 2439.872 kb on + strand, within IAI47_11530 at 2439.872 kb on + strand, within IAI47_11530 at 2439.872 kb on + strand, within IAI47_11530 at 2439.872 kb on + strand, within IAI47_11530 at 2439.872 kb on + strand, within IAI47_11530 at 2439.872 kb on + strand, within IAI47_11530 at 2439.872 kb on + strand, within IAI47_11530 at 2439.873 kb on - strand, within IAI47_11530 at 2439.873 kb on - strand, within IAI47_11530 at 2439.873 kb on - strand, within IAI47_11530 at 2439.873 kb on - strand, within IAI47_11530 at 2439.873 kb on - strand, within IAI47_11530 at 2439.873 kb on - strand, within IAI47_11530 at 2439.873 kb on - strand, within IAI47_11530 at 2439.873 kb on - strand, within IAI47_11530 at 2439.873 kb on - strand, within IAI47_11530 at 2439.874 kb on + strand, within IAI47_11530 at 2439.874 kb on + strand, within IAI47_11530 at 2439.874 kb on + strand, within IAI47_11530 at 2439.874 kb on + strand, within IAI47_11530 at 2439.874 kb on + strand, within IAI47_11530 at 2439.874 kb on + strand, within IAI47_11530 at 2439.874 kb on + strand, within IAI47_11530 at 2439.874 kb on + strand, within IAI47_11530 at 2439.874 kb on + strand, within IAI47_11530 at 2439.875 kb on - strand, within IAI47_11530 at 2439.875 kb on - strand, within IAI47_11530 at 2439.875 kb on - strand, within IAI47_11530 at 2439.875 kb on - strand, within IAI47_11530 at 2439.875 kb on - strand, within IAI47_11530 at 2439.875 kb on - strand, within IAI47_11530 at 2439.875 kb on - strand, within IAI47_11530 at 2439.897 kb on + strand, within IAI47_11530 at 2439.898 kb on - strand, within IAI47_11530 at 2439.921 kb on + strand, within IAI47_11530 at 2439.922 kb on - strand, within IAI47_11530 at 2440.021 kb on + strand, within IAI47_11530 at 2440.022 kb on - strand, within IAI47_11530 at 2440.134 kb on + strand, within IAI47_11530 at 2440.134 kb on + strand, within IAI47_11530 at 2440.197 kb on - strand, within IAI47_11530 at 2440.197 kb on - strand, within IAI47_11530 at 2440.197 kb on - strand, within IAI47_11530 at 2440.197 kb on - strand, within IAI47_11530 at 2440.294 kb on + strand, within IAI47_11530 at 2440.322 kb on - strand, within IAI47_11530 at 2440.351 kb on - strand, within IAI47_11530 at 2440.351 kb on - strand, within IAI47_11530 at 2440.351 kb on - strand, within IAI47_11530 at 2440.375 kb on + strand, within IAI47_11530 at 2440.375 kb on + strand, within IAI47_11530 at 2440.375 kb on + strand, within IAI47_11530 at 2440.425 kb on + strand, within IAI47_11530 at 2440.429 kb on - strand, within IAI47_11530 at 2440.431 kb on + strand, within IAI47_11530 at 2440.431 kb on + strand, within IAI47_11530 at 2440.432 kb on - strand, within IAI47_11530
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 2,437,593 - IAI47_11520 0.76 -0.1 2,437,593 - IAI47_11520 0.76 +0.0 2,437,759 - IAI47_11520 0.86 +0.3 2,438,043 + -2.2 2,438,069 + +0.7 2,438,279 - -0.7 2,438,299 - -1.1 2,438,329 + -0.3 2,438,354 + +0.5 2,439,471 + -0.3 2,439,472 - -0.0 2,439,473 + +0.1 2,439,520 - +1.5 2,439,540 - +0.3 2,439,540 - +2.8 2,439,541 + +0.8 2,439,541 + +0.5 2,439,542 - +1.0 2,439,572 - +0.0 2,439,572 - +1.5 2,439,616 + IAI47_11530 0.12 -0.9 2,439,617 - IAI47_11530 0.12 -0.3 2,439,621 + IAI47_11530 0.12 +0.0 2,439,621 + IAI47_11530 0.12 +0.1 2,439,648 + IAI47_11530 0.14 +0.6 2,439,648 + IAI47_11530 0.14 -1.2 2,439,648 + IAI47_11530 0.14 +0.5 2,439,649 - IAI47_11530 0.14 +1.5 2,439,649 - IAI47_11530 0.14 -1.6 2,439,649 - IAI47_11530 0.14 +0.9 2,439,655 - IAI47_11530 0.15 -0.2 2,439,679 - IAI47_11530 0.17 +0.1 2,439,679 - IAI47_11530 0.17 -0.2 2,439,730 - IAI47_11530 0.20 +0.1 2,439,731 + IAI47_11530 0.20 -1.4 2,439,745 - IAI47_11530 0.22 +1.8 2,439,745 - IAI47_11530 0.22 -2.1 2,439,745 - IAI47_11530 0.22 -1.8 2,439,745 - IAI47_11530 0.22 -0.9 2,439,747 + IAI47_11530 0.22 -0.1 2,439,747 + IAI47_11530 0.22 +0.4 2,439,774 + IAI47_11530 0.24 +0.1 2,439,775 - IAI47_11530 0.24 -0.2 2,439,775 - IAI47_11530 0.24 -0.2 2,439,775 - IAI47_11530 0.24 +0.5 2,439,775 - IAI47_11530 0.24 +0.1 2,439,775 - IAI47_11530 0.24 +0.7 2,439,775 - IAI47_11530 0.24 +0.6 2,439,796 + IAI47_11530 0.25 +1.2 2,439,796 + IAI47_11530 0.25 -1.9 2,439,797 - IAI47_11530 0.25 -1.6 2,439,797 - IAI47_11530 0.25 -0.3 2,439,808 + IAI47_11530 0.26 -0.5 2,439,826 + IAI47_11530 0.28 +0.5 2,439,826 + IAI47_11530 0.28 +1.1 2,439,826 + IAI47_11530 0.28 -0.5 2,439,826 + IAI47_11530 0.28 +0.7 2,439,826 + IAI47_11530 0.28 +2.3 2,439,826 + IAI47_11530 0.28 +0.3 2,439,827 - IAI47_11530 0.28 +2.1 2,439,827 - IAI47_11530 0.28 +0.6 2,439,827 - IAI47_11530 0.28 -1.3 2,439,827 - IAI47_11530 0.28 +0.1 2,439,827 - IAI47_11530 0.28 +1.5 2,439,827 - IAI47_11530 0.28 +0.0 2,439,827 - IAI47_11530 0.28 -1.8 2,439,838 + IAI47_11530 0.28 +1.2 2,439,839 - IAI47_11530 0.29 +0.7 2,439,839 - IAI47_11530 0.29 -1.9 2,439,839 - IAI47_11530 0.29 -0.9 2,439,839 - IAI47_11530 0.29 -0.3 2,439,839 - IAI47_11530 0.29 +1.0 2,439,839 - IAI47_11530 0.29 +0.5 2,439,849 - IAI47_11530 0.29 +0.7 2,439,864 + IAI47_11530 0.30 +1.0 2,439,866 + IAI47_11530 0.31 +0.0 2,439,866 + IAI47_11530 0.31 -0.5 2,439,866 + IAI47_11530 0.31 -0.7 2,439,866 + IAI47_11530 0.31 +0.3 2,439,867 - IAI47_11530 0.31 +0.6 2,439,872 + IAI47_11530 0.31 -2.5 2,439,872 + IAI47_11530 0.31 +0.2 2,439,872 + IAI47_11530 0.31 +0.3 2,439,872 + IAI47_11530 0.31 -1.0 2,439,872 + IAI47_11530 0.31 +0.2 2,439,872 + IAI47_11530 0.31 +0.3 2,439,872 + IAI47_11530 0.31 -1.6 2,439,872 + IAI47_11530 0.31 +1.7 2,439,873 - IAI47_11530 0.31 +1.0 2,439,873 - IAI47_11530 0.31 -0.1 2,439,873 - IAI47_11530 0.31 -1.8 2,439,873 - IAI47_11530 0.31 +0.6 2,439,873 - IAI47_11530 0.31 -0.6 2,439,873 - IAI47_11530 0.31 -0.3 2,439,873 - IAI47_11530 0.31 -0.3 2,439,873 - IAI47_11530 0.31 +0.9 2,439,873 - IAI47_11530 0.31 -1.1 2,439,874 + IAI47_11530 0.31 -0.5 2,439,874 + IAI47_11530 0.31 +0.8 2,439,874 + IAI47_11530 0.31 +0.5 2,439,874 + IAI47_11530 0.31 +0.5 2,439,874 + IAI47_11530 0.31 -0.7 2,439,874 + IAI47_11530 0.31 -1.3 2,439,874 + IAI47_11530 0.31 -1.3 2,439,874 + IAI47_11530 0.31 -1.6 2,439,874 + IAI47_11530 0.31 -0.5 2,439,875 - IAI47_11530 0.31 +0.2 2,439,875 - IAI47_11530 0.31 +0.6 2,439,875 - IAI47_11530 0.31 -0.3 2,439,875 - IAI47_11530 0.31 +0.9 2,439,875 - IAI47_11530 0.31 +0.8 2,439,875 - IAI47_11530 0.31 -1.5 2,439,875 - IAI47_11530 0.31 -0.2 2,439,897 + IAI47_11530 0.33 -1.8 2,439,898 - IAI47_11530 0.33 -0.5 2,439,921 + IAI47_11530 0.35 -0.4 2,439,922 - IAI47_11530 0.35 +0.4 2,440,021 + IAI47_11530 0.42 -0.5 2,440,022 - IAI47_11530 0.42 +0.4 2,440,134 + IAI47_11530 0.51 -1.0 2,440,134 + IAI47_11530 0.51 -2.2 2,440,197 - IAI47_11530 0.55 +1.5 2,440,197 - IAI47_11530 0.55 -0.2 2,440,197 - IAI47_11530 0.55 +0.3 2,440,197 - IAI47_11530 0.55 -0.7 2,440,294 + IAI47_11530 0.63 -0.2 2,440,322 - IAI47_11530 0.65 -0.1 2,440,351 - IAI47_11530 0.67 -1.9 2,440,351 - IAI47_11530 0.67 +0.1 2,440,351 - IAI47_11530 0.67 +2.3 2,440,375 + IAI47_11530 0.69 +0.2 2,440,375 + IAI47_11530 0.69 +1.5 2,440,375 + IAI47_11530 0.69 +0.6 2,440,425 + IAI47_11530 0.72 -0.9 2,440,429 - IAI47_11530 0.73 +0.1 2,440,431 + IAI47_11530 0.73 +1.0 2,440,431 + IAI47_11530 0.73 +1.3 2,440,432 - IAI47_11530 0.73 -0.1
Or see this region's nucleotide sequence